Carg02315 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02315
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionsirohydrochlorin ferrochelatase, chloroplastic-like
LocationCarg_Chr15: 1797747 .. 1800149 (-)
RNA-Seq ExpressionCarg02315
SyntenyCarg02315
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAATATATACTTTTCTCTATCTAAACGACATCGTTTTCCAGGTCAATCGTTCCAGACAGGCGGCCTCTGCAAAGGATCGGAACCCATGACATCGTTATCCATCCCTTCTCTAATTACAGTGAAGAGGTCTGAAAGTCTTTGTCCTAATTTTCTTTTCACTTTGCTTGATTTTCCTCTAATGGCCGTTCGTTTCTCCATTGAAGTCCTGCAGCAGGGGAAACTAGAAAAACGTATACAATCTGCGCATCTCAGAAATCTTCAATTCTGATCGGAGTCGGACGGTCCTCTACATTAAGGAATTTCTCCTCGCGGCTCCGCATGAGTGTGGATAATGGAGGCGCTAATCGGATTGGTTCTGGAGATGCCGTGATCATTGTCGACCATGGCTCTCGTCGCCGGGAATCTAACCTCATGCTGAGTAAGCTAGGGTTTCGAATTTTGGAATTTGCCTGAAATTTTAAGTTCTATGTAGGCTAATCCTCTTGTGATTCTGAATTTGTTGAAAATTTCAGATGAATTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAACCTGCTCACATGGTATGTTCTTATGAAATATGTATAGGTTTATGGATATAATTTTTCCTACACTTTGAAGTACAACATCTCTGGCCTTCAAGATAACATAGGACCCAATGAATACAGAATACAAAATAAATAATGATACCCTTAAATATGAATAGTGGTATGGAACTGTAACTGCCCAAGACCATCGATAGGAGATATTGTGTTCTTTGAGCTTTTGCTTAAGATTTGGGTCTGCTAAGAAGAGGTTTCTACACCTTTATAAAGAATGTTTTGAACTCACAATCCACCCCTTTTAGGCCCAGGAGAGTCCTTGCGGGCACGCCGCCCTCACGTCCACCCCCTTTAGAGCTCGGCCTCCTCGTTGGTACATCGCCCGATATCTGACTCTGATATCATTTGTAACAGCCCAAGCCCATCACTAGTAGATATTGTCTTCTTTGAGCTTTCCCTTTCGGGCTTTCTCTCAGCCTGAACCCACCACTATCAGATATTGTCTTCTTTGAGCTTTTCCTTAAGGCTTGGGTCTGCTAGGGAGGGGTTTCCACACCCTTATAAAGAATGTTTCGATCTCACAATCCACCCCTTTCAAGTCCAAGAGCGTGCTTGCTGGCACACCGCCTCATGTCCACCCCCTTTAGAGCTCAGCCTCCTTGTTGGTACATCGCCCAGTATCTGACTCTGATATCATTTGTAACAGCCCAAGCCCATCAGTAGCAGATATTGTCGTCTTTGAGTTTTTCCTTCCAAGTTTTCCCTCATGGTTTTTAAAATGCATGTGCGAGGGAGAGGTTTTCACGCCCTAATAAAGAACTGCCAGTAGTTTCTTTGTCTTTTTCATCCCTTTCCATTTCTTCCCATTGCACATATGTTTCAACACATGATCTTAAAATAATTTGATTAGAAAGAGTAACACTCACAACCATAACCATGTCTATTGTACTTATGGTTTCCTGGCTTCCAGTTATTCTTAGATATGTTTGGAAGTTGAGCTGGGGGGTTCTAATGATTTCTCTTTTACTTTGTTCTAAAACAACACAGGAGTTGGCTGAACCGTCGATAAGAGACTCATTTGCATCGTGTGTCGAACAGGGGGCCAAACGAATCGTTGTAAGCCCGTTCTTTCTCTTCCCCGGTCGACATTGGCAACAGGTAACTCACTGTGGGACGCCTAATTTGATGAAATGAGCTGGTGGGCCTTTGATTCTTCTATGATTGTTTGGGTAAGAGGATTGATAGCAATGGCCAATGGGTGATGTTTTGCAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCGGGAATATCGTATATAATAACTGCTCCTCTCGGTCTTCATGAACAGCTTGTGGTACGAATTTCTGAAGCACATTTTTTTAGAGTATAGGATAGAGGAAAGCAAGGTGATAGATGATAAAATGAACTGTGAGGTGTTCTGTTGGCAGGATGTTGTGAATGATAGAATAAGTCACTGCTTAAGCCATGCTGCTGGAGTTGTAGAAGAATGTGAAGTCTGTGCAGGAACAGGCAAATGCAAATTTAATTGACTTGGGATGAAGGGCCTATAACAATGTGGAGAAGCTACAGAAGGGGATAATAAGGGTTTCCAGGTATCAATGGCTTGAGAATCTATCTATCTTAGTTGATATCAATGCTTTGTAATACTTGACCATTTTGGTTGTTTTTCTTTGTTGTATTTTCACATATGACTACAATATGCTCAATAATATTTGTTTTCAATGGTTTATATGCTTTCAAAGTCGGTTGGTGTTGTTGCCTAATCGCTTTTAGTTTATAACATTGTTTTCTTTCAAATTTTGATTTGAGGCTCGAGC

mRNA sequence

AGAATATATACTTTTCTCTATCTAAACGACATCGTTTTCCAGGTCAATCGTTCCAGACAGGCGGCCTCTGCAAAGGATCGGAACCCATGACATCGTTATCCATCCCTTCTCTAATTACAGTGAAGAGTCCTGCAGCAGGGGAAACTAGAAAAACGTATACAATCTGCGCATCTCAGAAATCTTCAATTCTGATCGGAGTCGGACGGTCCTCTACATTAAGGAATTTCTCCTCGCGGCTCCGCATGAGTGTGGATAATGGAGGCGCTAATCGGATTGGTTCTGGAGATGCCGTGATCATTGTCGACCATGGCTCTCGTCGCCGGGAATCTAACCTCATGCTGAATGAATTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAACCTGCTCACATGGAGTTGGCTGAACCGTCGATAAGAGACTCATTTGCATCGTGTGTCGAACAGGGGGCCAAACGAATCGTTGTAAGCCCGTTCTTTCTCTTCCCCGGTCGACATTGGCAACAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCGGGAATATCGTATATAATAACTGCTCCTCTCGGTCTTCATGAACAGCTTGTGGATGTTGTGAATGATAGAATAAGTCACTGCTTAAGCCATGCTGCTGGAGTTGTAGAAGAATGTGAAGTCTGTGCAGGAACAGGCAAATGCAAATTTAATTGACTTGGGATGAAGGGCCTATAACAATGTGGAGAAGCTACAGAAGGGGATAATAAGGGTTTCCAGGTATCAATGGCTTGAGAATCTATCTATCTTAGTTGATATCAATGCTTTGTAATACTTGACCATTTTGGTTGTTTTTCTTTGTTGTATTTTCACATATGACTACAATATGCTCAATAATATTTGTTTTCAATGGTTTATATGCTTTCAAAGTCGGTTGGTGTTGTTGCCTAATCGCTTTTAGTTTATAACATTGTTTTCTTTCAAATTTTGATTTGAGGCTCGAGC

Coding sequence (CDS)

ATGACATCGTTATCCATCCCTTCTCTAATTACAGTGAAGAGTCCTGCAGCAGGGGAAACTAGAAAAACGTATACAATCTGCGCATCTCAGAAATCTTCAATTCTGATCGGAGTCGGACGGTCCTCTACATTAAGGAATTTCTCCTCGCGGCTCCGCATGAGTGTGGATAATGGAGGCGCTAATCGGATTGGTTCTGGAGATGCCGTGATCATTGTCGACCATGGCTCTCGTCGCCGGGAATCTAACCTCATGCTGAATGAATTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAACCTGCTCACATGGAGTTGGCTGAACCGTCGATAAGAGACTCATTTGCATCGTGTGTCGAACAGGGGGCCAAACGAATCGTTGTAAGCCCGTTCTTTCTCTTCCCCGGTCGACATTGGCAACAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCGGGAATATCGTATATAATAACTGCTCCTCTCGGTCTTCATGAACAGCTTGTGGATGTTGTGAATGATAGAATAAGTCACTGCTTAAGCCATGCTGCTGGAGTTGTAGAAGAATGTGAAGTCTGTGCAGGAACAGGCAAATGCAAATTTAATTGA

Protein sequence

MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGANRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAAGVVEECEVCAGTGKCKFN
Homology
[Close]
BLAST of Carg02315 vs. NCBI nr
Match: KAG7016202.1 (Sirohydrochlorin ferrochelatase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 415.2 bits (1066), Expect = 3.2e-112
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA
Sbjct: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC
Sbjct: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           ISHCLSHAAGVVEECEVCAGTGKCKFN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Carg02315 vs. NCBI nr
Match: KAG6578662.1 (Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 411.8 bits (1057), Expect = 3.6e-111
Identity = 205/207 (99.03%), Postives = 206/207 (99.52%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           MTSLSIPS ITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA
Sbjct: 1   MTSLSIPSQITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           NRIG+GDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           ISHCLSHAAGVVEECEVCAGTGKCKFN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Carg02315 vs. NCBI nr
Match: XP_022939639.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 407.1 bits (1045), Expect = 8.8e-110
Identity = 202/207 (97.58%), Postives = 205/207 (99.03%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           MTSLSIPS ITVKSP+AGETRKTYTICASQKSSILIGVGRSSTLRNFSSR RMSVDNGGA
Sbjct: 1   MTSLSIPSQITVKSPSAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRFRMSVDNGGA 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           +RIG+GDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC
Sbjct: 61  DRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           ISHCLSHAAGVVEECEVCAGTGKCKFN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Carg02315 vs. NCBI nr
Match: XP_022993758.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 405.6 bits (1041), Expect = 2.6e-109
Identity = 200/207 (96.62%), Postives = 204/207 (98.55%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           MTSLSIP  ITVKSP+AGETRKTYTICASQKSSILIG+GRSSTLRNFSSR RMSVDNGG+
Sbjct: 1   MTSLSIPPQITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGS 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           NRIG+GDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           ISHCLSHAAGVVEECEVCAGTGKCKFN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Carg02315 vs. NCBI nr
Match: XP_023551685.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 405.2 bits (1040), Expect = 3.4e-109
Identity = 201/207 (97.10%), Postives = 204/207 (98.55%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           MTSLSIPS ITVKSP+AGETRKTYTICASQKS ILIGVGRSSTLRNFSSR RMSVDNGGA
Sbjct: 1   MTSLSIPSQITVKSPSAGETRKTYTICASQKSPILIGVGRSSTLRNFSSRFRMSVDNGGA 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           +RIG+GDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC
Sbjct: 61  DRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           ISHCLSHAAGVVEECEVCAGTGKCKFN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Carg02315 vs. ExPASy Swiss-Prot
Match: Q84JH7 (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIRB PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.4e-62
Identity = 111/145 (76.55%), Postives = 124/145 (85.52%), Query Frame = 0 

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           N IG  D +IIVDHGSRRRESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ C
Sbjct: 76  NGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLC 135

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           V+QGAKR+VVSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L+DVVNDR
Sbjct: 136 VQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDR 195

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCK 206
           I HCLSH  G  +EC VCAGT KCK
Sbjct: 196 IQHCLSHVEGDADECLVCAGTNKCK 220

[Close]
BLAST of Carg02315 vs. ExPASy Swiss-Prot
Match: P61817 (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.7e-15
Identity = 40/116 (34.48%), Postives = 62/116 (53.45%), Query Frame = 0 

Query: 67  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 126
           DAV+ V HGSR +E       F+   +     PI E   +ELA P+I   F +C+EQGA 
Sbjct: 13  DAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELASPTIEQGFEACIEQGAT 72

Query: 127 RIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIS 183
           RI + P  L    H + DIP    +  + +P +  +   P G+ E++VD++ +RI+
Sbjct: 73  RIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVDERIVDILVERIN 128

[Close]
BLAST of Carg02315 vs. ExPASy Swiss-Prot
Match: Q58380 (Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=cbiX PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.4e-12
Identity = 42/138 (30.43%), Postives = 66/138 (47.83%), Query Frame = 0 

Query: 67  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 126
           +A+++V HGSR   S  +L +     +++  +PIVE   ME +EP+I  +    +EQGAK
Sbjct: 2   EALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAK 61

Query: 127 RIVVSPFFLFPGRHWQQDIPSLTAEAAKDH------------------------PGISYI 181
           RI+V P FL  G H  +DIP L      +H                          +  I
Sbjct: 62  RIIVVPVFLAHGIHTTRDIPRLLGLIEDNHEHHHEHSHHHHHHHHHEHEKLEIPEDVEII 121

[Close]
BLAST of Carg02315 vs. ExPASy Swiss-Prot
Match: P61819 (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=cbiX PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.4e-12
Identity = 39/139 (28.06%), Postives = 66/139 (47.48%), Query Frame = 0 

Query: 67  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 126
           +A+++V HGSR   S  ++ E     + +  Y IVE   ME  EP+I  +    ++ GAK
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 127 RIVVSPFFLFPGRHWQQDIPSLTA------EAAKDH-------------------PGISY 181
           +++V+P FL PG H ++DIP +        E    H                    G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

[Close]
BLAST of Carg02315 vs. ExPASy Swiss-Prot
Match: A6UWT5 (Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=cbiX PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.4e-11
Identity = 39/138 (28.26%), Postives = 66/138 (47.83%), Query Frame = 0 

Query: 67  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 126
           +A++++ HGSR   S  ++ +     ++K  Y IVE   ME  EP+I  +    + +GAK
Sbjct: 2   EALVLLGHGSRLPYSKEIVGKVAEKIKEKNIYDIVEIGMMEFNEPTIPQTINKVIAEGAK 61

Query: 127 RIVVSPFFLFPGRHWQQDIPSLTA--EAAKDH----------------------PGISYI 181
           +I++ P FL  G H ++DIP +    E  + H                       G+  I
Sbjct: 62  KIIIVPVFLAHGNHTKRDIPQILGLIECEEHHHEGEGGHHHHHHHHHGEKIEVPEGVEII 121

[Close]
BLAST of Carg02315 vs. ExPASy TrEMBL
Match: A0A6J1FHT5 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445470 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 4.3e-110
Identity = 202/207 (97.58%), Postives = 205/207 (99.03%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           MTSLSIPS ITVKSP+AGETRKTYTICASQKSSILIGVGRSSTLRNFSSR RMSVDNGGA
Sbjct: 1   MTSLSIPSQITVKSPSAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRFRMSVDNGGA 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           +RIG+GDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC
Sbjct: 61  DRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           ISHCLSHAAGVVEECEVCAGTGKCKFN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Carg02315 vs. ExPASy TrEMBL
Match: A0A6J1K384 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489668 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.2e-109
Identity = 200/207 (96.62%), Postives = 204/207 (98.55%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           MTSLSIP  ITVKSP+AGETRKTYTICASQKSSILIG+GRSSTLRNFSSR RMSVDNGG+
Sbjct: 1   MTSLSIPPQITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGS 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           NRIG+GDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           ISHCLSHAAGVVEECEVCAGTGKCKFN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Carg02315 vs. ExPASy TrEMBL
Match: A0A5D3BY55 (Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00520 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.2e-93
Identity = 172/207 (83.09%), Postives = 188/207 (90.82%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           M+SLS+   I+ KSP+  ETR  YTICA Q+S   I +GR S  RNF+SR+RMS+DNG +
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICAFQRSP--IPIGRLSKFRNFTSRVRMSIDNGSS 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           NRIG+GDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SC
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDR
Sbjct: 121 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           I+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 INHCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of Carg02315 vs. ExPASy TrEMBL
Match: A0A1S3C8S9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498093 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.2e-93
Identity = 172/207 (83.09%), Postives = 188/207 (90.82%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           M+SLS+   I+ KSP+  ETR  YTICASQ+S   I +GR S  RNF+SR+RMS+DNG +
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSP--IPIGRLSKFRNFTSRVRMSIDNGSS 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           NRIG+GDAVIIVDHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SC
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDR
Sbjct: 121 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           I+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 INHCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of Carg02315 vs. ExPASy TrEMBL
Match: A0A0A0KP17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.4e-91
Identity = 169/207 (81.64%), Postives = 187/207 (90.34%), Query Frame = 0 

Query: 1   MTSLSIPSLITVKSPAAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRLRMSVDNGGA 60
           M+S SI   I+ KS +  ETR+ ++ICASQ+SS  I VGR S  RNF+SR RMS+DNG +
Sbjct: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSS--IPVGRLSKFRNFTSRFRMSIDNGSS 60

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           NRIG+GDAVIIVDHGSRRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSF  C
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLC 120

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 208
           I++CLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 INYCLSHAAGVSDECEVCAGTGKCRFN 205

[Close]
BLAST of Carg02315 vs. TAIR 10
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B )

HSP 1 Score: 240.7 bits (613), Expect = 1.0e-63
Identity = 111/145 (76.55%), Postives = 124/145 (85.52%), Query Frame = 0 

Query: 61  NRIGSGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120
           N IG  D +IIVDHGSRRRESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ C
Sbjct: 76  NGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLC 135

Query: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           V+QGAKR+VVSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L+DVVNDR
Sbjct: 136 VQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDR 195

Query: 181 ISHCLSHAAGVVEECEVCAGTGKCK 206
           I HCLSH  G  +EC VCAGT KCK
Sbjct: 196 IQHCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:
BLAST of Carg02315 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7016202.13.2e-112100.00Sirohydrochlorin ferrochelatase, chloroplastic [Cucurbita argyrosperma subsp. ar... [more]
KAG6578662.13.6e-11199.03Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma ... [more]
XP_022939639.18.8e-11097.58sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita moschata][more]
XP_022993758.12.6e-10996.62sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima][more]
XP_023551685.13.4e-10997.10sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
BLAST of Carg02315 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q84JH71.4e-6276.55Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
P618171.7e-1534.48Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1[more]
Q583801.4e-1230.43Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC ... [more]
P618191.4e-1228.06Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) ... [more]
A6UWT54.4e-1128.26Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-128... [more]
BLAST of Carg02315 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FHT54.3e-11097.58sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=366... [more]
A0A6J1K3841.2e-10996.62sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 ... [more]
A0A5D3BY551.2e-9383.09Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C8S91.2e-9383.09sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KP174.4e-9181.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1[more]
BLAST of Carg02315 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G50170.11.0e-6376.55sirohydrochlorin ferrochelatase B [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.1400coord: 65..188
e-value: 1.3E-34
score: 121.0
NoneNo IPR availablePANTHERPTHR33542:SF3SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 2..204
NoneNo IPR availablePANTHERPTHR33542SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 2..204
NoneNo IPR availableCDDcd03416CbiX_SirB_Ncoord: 68..168
e-value: 1.58697E-38
score: 126.532
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 66..184
IPR002762Sirohydrochlorin cobaltochelatase CbiX-likePFAMPF01903CbiXcoord: 74..178
e-value: 2.4E-30
score: 105.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02315-RACarg02315-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006979 response to oxidative stress
biological_process GO:0019354 siroheme biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0042802 identical protein binding
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051266 sirohydrochlorin ferrochelatase activity
molecular_function GO:0016829 lyase activity