Carg02314 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02314
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Isocitrate dehydrogenase [NADP]
Location	Carg_Chr15: 1793825 .. 1797087 (-)
RNA-Seq Expression	Carg02314
Synteny	Carg02314

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGCAAGACTCATACGAACCGCCATGTCAAGTGCCAGTCTCTTCTCTTCATCCGTTGCTCTCAAAGACCCTTCGTTGCGGTTCTCCTTGACCTCTTCTACTCTCTTCAATGGACGCCTTCTCAGGAATCAGCTTCCCTGTCCCATTCGCTCCCCTGCTGTTTCGCATCGATGCTACGCATCTTCTTCTGGTTTTGATCGAGTTCGCGTCTCGGGTCCTGTTGTTGAAATGGACGGTAAGGTATCATGTTTGTGGTCTGTCATTCCATTGTTATCAAAGTGAATTGAGTGAACTTTGTATTGTATTCTAGTTAAAACTCATTGAACTCTGTTTTTTTTCCGCTTTGAACATTTATATATGTGCTTGACTTTTCTAGTTATGATTATTGAATTATTCAGATTCATGTGTTTGTAAACTTGTCTTTTCCATTTTTATACTTGTTACAGCTTCACTGATTTTCATTTTTTCTTTCCTTTACAATTAGGTGATGAAATGACGAGGATCATATGGACAATGATAAAAGAGAAAGTATGTTTACTTTTGAACGAAATTTTTTCGATTTTAATCTTTTGGAGAATAATGATTTTTCCTTCATAACATTGTAGCTTATTTTCCCGTTCCTGGATTTGGATATAAAATATTATGACTTGGGGATATTGAATCGGGACGCCACTAATGACAGAGTTACCATAGAGAGTGCAGAAGCAACTCTTAAGTATGTTCTGCTTGTATTCAACTATTATATATTTTGGATATTCCTATTTTGGATTCAACATTATTTATTTGATTGTGCTAAATGGCTTGGCCGTTCAAAAGTGGTAGGAAGTTGTTCAAACTATGAATGATGTGCTTGTGGATGGAAAGTATAAGAAAAGTATAAGCTTCTTAATAATATTACATTTTGTACAGATACTGTTTTCCGAGAACCTATTTATGTCAAAACATCCCGAGAATTGTTCCTGGTAATTTTCATTGTCTAACCTTTTGTCTTGTAATCCATTATGAAACATGATATAATACTTGGTTAATACAGGTTGGAAGAAGCCCATATGTATTGGTAGGCATGCTTTTGGTGATCAAAACCGTGCCACTGATACTATCATCCCAGGGCCTGGAAAGCTTAAAATGGTGTTTGGTGAGATAATTTACTATCATCCCAGGGCCTGGAAAGCTTAAAATGGTGTTTGGTGAGATAATTTACTATCATCCCAGGCCTGGAAAGCTTAAAATGGTGTTTGGTGGGATAATTTACTACCATCCCAGGGCCTGGAAAGCTTAAAATGGTGTTTGGTGAAATAATTTTCTTTTCTTCACTTATTCTCATGTATTGCTCTGGACAACTGTTTTCTCATCCCCAAACTTTTTATTATCTTTCAACTCTTTTCTTTTTTTGTTTCTATGCAGTTCCAGAAAATGTGGAGACACCGAGAGAGCTCGAGGTTTATGACTTCAAAGGACCAGGCATTGGGCTTACAATGTATAATGTTGACGAGGTTGGTTTTATTTTTTCGTACTTGTTGATTTTTTGTATCACATAAATTACTCTTTTAACTCGTTCCTATGCAATTGGCAGTCTATTCAAGCATTTGCAGAGTCGTCAATGTCACTGGCATTTGAGAAGAAGTGGCCCTTGTATCTGAGCACTAAGAATACAATTCTTAAGAAATATGATGGCAGGTATTTCTTACTTCGTTCCCTGTACCTAGTTGCTTATGCTGTTTATATCTACTTTCTGTTTCCCAGAAATCGTCAAAGTGATCATTTATTTTAATACTGCCCTGCAGGTTTAAAGATATATTTCAAGAAGTGTACGAGGAAAAGTGGAAGCAGAAGTTTGAAGAAAATTTGATATGGTTCATTACATTTAAATACATGATTCTCTACAGTTATGCCTAATCTATTTGCCTATGGCTGAATATATTTTTCTTCGGCATTTAGGTACGAACATTGGCTTATTGATGACATGGTAGCTTAGGCTATAAAAAGTCAAGGTGGATACGTTTGAGCTTGCAAAAATTATGATGGGGACGTTCAGAGTGATTTACTCGCACAGGGTGATGCTATCAACTTTTCTAATTGCCTGGGTTCTCTTATATTACATGCTTTTATAACTTTTAATATCAATAATTTCTAACAATATTATTAACTACAGAACTTCTACTTCGTCATACCTTGTTTTCATTCTTAAGGTCTTTCATTGATCAGAGAATGCAAGTTACTTCTCGACATTAACATGTCACGTATTTCGTTGTTGATAAAAATGATCTCTGTTGTTTTCGTTATGGTTTGCATCGAGTCGAATGCTTATTTTCCATTGGCAGGATTTGGTAATTTGGGGCTAATGACTTCTGTGTTGGTGGGTACTTAATACTTTAGAATGTGAAATTTATCGTCTCCATGGACTAATTTTTTTTCATAAGGAACGCCTTAAAAGTTGAGATTTATTGCAGTTGTCATCTGATGGGAAGACATTGGAAGCCGAGGCGGCTCATGGCACTGTAATGCGGCATTACCGATTACATCAAAAGGGACAAGAAACCAGTACAAATAGTATTGCCTCTATTTTTGCATGGACACGGGGCTTTGAGCATAGTATTAAAATATTGCAGGTTGTTCATGTCCACGATGGTATGATATTGTCCACTTTGTGCATAAGTTCTCATGGCTTTACTTTGGGCTTCCCAAAAAAGCTTTATACCAATGGAGAGAGTATTCCTCACTTATAAATTCATGATCTTCTACTAAATTAACCAATGTGGGACTCACTCTAAATAATCCTCAACCGTTCGTATATATTTTTTTGTGGTTCTTGTTCCATCTAAATAGCGTTGGGAATATAGTTCTGCTTTTCACTAGAGAGAGTCACCATGACCAACCACTTTACGATTCGATACATATTCGTAATGGGGCTTTGTAGAGTAGTGTACTACCTAGAAACTGACAATTTTTAGCTGTATTTAAATGTCTTCAGGGCCAAATTAGATAAAAACGAGAGGCTTCATACATTGAGACGGTGGAATCGGGGAAAATGACAAAAGGATCTTGCCTTGCTGATATATGGACCCAAGTAACAATTTAAACATTTCATCTCATTCACCTCTCTTGACATTTGTAGTTTGGAATTACTTAGATGTGTACGATTTTTGCAGGGTATCAAGGGAGTTTTACTTAAACACAGAGGAGTTCATCGACACCGTAGCACACAATCTGGTAACGAAACTCCAGCGACCCCTTGTAACGGTCAACTAAATGACCTCCAG

mRNA sequence

ATGAAAGCAAGACTCATACGAACCGCCATGTCAAGTGCCAGTCTCTTCTCTTCATCCGTTGCTCTCAAAGACCCTTCGTTGCGGTTCTCCTTGACCTCTTCTACTCTCTTCAATGGACGCCTTCTCAGGAATCAGCTTCCCTGTCCCATTCGCTCCCCTGCTGTTTCGCATCGATGCTACGCATCTTCTTCTGGTTTTGATCGAGTTCGCGTCTCGGGTCCTGTTGTTGAAATGGACGGTGATGAAATGACGAGGATCATATGGACAATGATAAAAGAGAAACTTATTTTCCCGTTCCTGGATTTGGATATAAAATATTATGACTTGGGGATATTGAATCGGGACGCCACTAATGACAGAGTTACCATAGAGAGTGCAGAAGCAACTCTTAAATACTGTTTTCCGAGAACCTATTTATGTCAAAACATCCCGAGAATTGTTCCTGGTTGGAAGAAGCCCATATGTATTGGTAGGCATGCTTTTGGTGATCAAAACCGTGCCACTGATACTATCATCCCAGGGCCTGGAAAGCTTAAAATGGTGTTTGTTCCAGAAAATGTGGAGACACCGAGAGAGCTCGAGGTTTATGACTTCAAAGGACCAGGCATTGGGCTTACAATGTATAATGTTGACGAGTCTATTCAAGCATTTGCAGAGTCGTCAATGTCACTGGCATTTGAGAAGAAGTGGCCCTTGTATCTGAGCACTAAGAATACAATTCTTAAGAAATATGATGGCAGGTTTAAAGATATATTTCAAGAAGTGTACGAGGAAAAGTGGAAGCAGAAGTTTGAAGAAAATTTGATATGGTACGAACATTGGCTTATTGATGACATGTTGTCATCTGATGGGAAGACATTGGAAGCCGAGGCGGCTCATGGCACTGGTATCAAGGGAGTTTTACTTAAACACAGAGGAGTTCATCGACACCGTAGCACACAATCTGGTAACGAAACTCCAGCGACCCCTTGTAACGGTCAACTAAATGACCTCCAG

Coding sequence (CDS)

ATGAAAGCAAGACTCATACGAACCGCCATGTCAAGTGCCAGTCTCTTCTCTTCATCCGTTGCTCTCAAAGACCCTTCGTTGCGGTTCTCCTTGACCTCTTCTACTCTCTTCAATGGACGCCTTCTCAGGAATCAGCTTCCCTGTCCCATTCGCTCCCCTGCTGTTTCGCATCGATGCTACGCATCTTCTTCTGGTTTTGATCGAGTTCGCGTCTCGGGTCCTGTTGTTGAAATGGACGGTGATGAAATGACGAGGATCATATGGACAATGATAAAAGAGAAACTTATTTTCCCGTTCCTGGATTTGGATATAAAATATTATGACTTGGGGATATTGAATCGGGACGCCACTAATGACAGAGTTACCATAGAGAGTGCAGAAGCAACTCTTAAATACTGTTTTCCGAGAACCTATTTATGTCAAAACATCCCGAGAATTGTTCCTGGTTGGAAGAAGCCCATATGTATTGGTAGGCATGCTTTTGGTGATCAAAACCGTGCCACTGATACTATCATCCCAGGGCCTGGAAAGCTTAAAATGGTGTTTGTTCCAGAAAATGTGGAGACACCGAGAGAGCTCGAGGTTTATGACTTCAAAGGACCAGGCATTGGGCTTACAATGTATAATGTTGACGAGTCTATTCAAGCATTTGCAGAGTCGTCAATGTCACTGGCATTTGAGAAGAAGTGGCCCTTGTATCTGAGCACTAAGAATACAATTCTTAAGAAATATGATGGCAGGTTTAAAGATATATTTCAAGAAGTGTACGAGGAAAAGTGGAAGCAGAAGTTTGAAGAAAATTTGATATGGTACGAACATTGGCTTATTGATGACATGTTGTCATCTGATGGGAAGACATTGGAAGCCGAGGCGGCTCATGGCACTGGTATCAAGGGAGTTTTACTTAAACACAGAGGAGTTCATCGACACCGTAGCACACAATCTGGTAACGAAACTCCAGCGACCCCTTGTAACGGTCAACTAAATGACCTCCAG

Protein sequence

MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCYASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDRVTIESAEATLKYCFPRTYLCQNIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGPGIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWKQKFEENLIWYEHWLIDDMLSSDGKTLEAEAAHGTGIKGVLLKHRGVHRHRSTQSGNETPATPCNGQLNDLQ

Homology

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BLAST of Carg02314 vs. NCBI nr
Match: KAG7016201.1 (Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681.8 bits (1758), Expect = 3.0e-192
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60
           MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY
Sbjct: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60

Query: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120
           ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR
Sbjct: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120

Query: 121 VTIESAEATLKYCFPRTYLCQNIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKM 180
           VTIESAEATLKYCFPRTYLCQNIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKM
Sbjct: 121 VTIESAEATLKYCFPRTYLCQNIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKM 180

Query: 181 VFVPENVETPRELEVYDFKGPGIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTI 240
           VFVPENVETPRELEVYDFKGPGIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTI
Sbjct: 181 VFVPENVETPRELEVYDFKGPGIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTI 240

Query: 241 LKKYDGRFKDIFQEVYEEKWKQKFEENLIWYEHWLIDDMLSSDGKTLEAEAAHGTGIKGV 300
           LKKYDGRFKDIFQEVYEEKWKQKFEENLIWYEHWLIDDMLSSDGKTLEAEAAHGTGIKGV
Sbjct: 241 LKKYDGRFKDIFQEVYEEKWKQKFEENLIWYEHWLIDDMLSSDGKTLEAEAAHGTGIKGV 300

Query: 301 LLKHRGVHRHRSTQSGNETPATPCNGQLNDLQ 333
           LLKHRGVHRHRSTQSGNETPATPCNGQLNDLQ
Sbjct: 301 LLKHRGVHRHRSTQSGNETPATPCNGQLNDLQ 332

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BLAST of Carg02314 vs. NCBI nr
Match: KAG6578661.1 (Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 552.4 bits (1422), Expect = 2.7e-153
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60
           MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY
Sbjct: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60

Query: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120
           ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR
Sbjct: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120

Query: 121 VTIESAEATLKYCFPRTYLCQNIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKM 180
           VTIESAEATLKYCFPRTYLCQNIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKM
Sbjct: 121 VTIESAEATLKYCFPRTYLCQNIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKM 180

Query: 181 VFVPENVETPRELEVYDFKGPGIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTI 240
           VFVPENVETPRELEVYDFKGPGIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTI
Sbjct: 181 VFVPENVETPRELEVYDFKGPGIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTI 240

Query: 241 LKKYDGRFKDIFQEVYEEKWKQKFEENLIW 271
           LKKYDGRFKDIFQEVYEEKWKQKFEENLIW
Sbjct: 241 LKKYDGRFKDIFQEVYEEKWKQKFEENLIW 270

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BLAST of Carg02314 vs. NCBI nr
Match: XP_023515364.1 (isocitrate dehydrogenase [NADP] [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496.9 bits (1278), Expect = 1.4e-136
Identity = 268/386 (69.43%), Postives = 276/386 (71.50%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60
           MKARLIRTAMSSA+L SSS+ALK+PS  FS T ST FNGRLL+NQLPCPIR PAVS RCY
Sbjct: 1   MKARLIRTAMSSATLLSSSLALKNPSFHFSSTFSTPFNGRLLKNQLPCPIRFPAVSLRCY 60

Query: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120
           ASSSG DRVRV  PVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDAT+DR
Sbjct: 61  ASSSGVDRVRVLNPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATDDR 120

Query: 121 VTIESAEATLKY---------------------------------------CFPRTYLCQ 180
           VT+ESAEATLKY                                        F    LCQ
Sbjct: 121 VTVESAEATLKYNVAVKCATITPDEARVKEFGLKAMWRSPNGTIRNILNGTVFREPILCQ 180

Query: 181 NIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP 240
           NIPRIVPGWKKPICIGRHAFGDQ RATDTIIPGPGKLKMVFVPEN ETPRELEVYDFKGP
Sbjct: 181 NIPRIVPGWKKPICIGRHAFGDQYRATDTIIPGPGKLKMVFVPENGETPRELEVYDFKGP 240

Query: 241 GIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 300
           GI L MYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK
Sbjct: 241 GIALAMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 300

Query: 301 QKFEENLIWYEHWLIDDM-----------------------------------------L 307
           QKFEE  IWYEH LIDDM                                         L
Sbjct: 301 QKFEEYSIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLL 360

[Close]

BLAST of Carg02314 vs. NCBI nr
Match: XP_022963180.1 (isocitrate dehydrogenase [NADP]-like [Cucurbita moschata])

HSP 1 Score: 496.9 bits (1278), Expect = 1.4e-136
Identity = 268/386 (69.43%), Postives = 276/386 (71.50%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60
           MKARLIRTAMSSA+L SSS+ALK+P   FS T ST FNGRLL+NQLPCPIR PAVS RCY
Sbjct: 1   MKARLIRTAMSSATLLSSSLALKNPCFHFSSTFSTPFNGRLLKNQLPCPIRFPAVSLRCY 60

Query: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120
           ASSSG DRVRV  PVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDAT+DR
Sbjct: 61  ASSSGVDRVRVLNPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATDDR 120

Query: 121 VTIESAEATLKY---------------------------------------CFPRTYLCQ 180
           VT+ESAEATLKY                                        F    LCQ
Sbjct: 121 VTVESAEATLKYNVAVKCATITPDEARVKEFGLKAMWRSPNGTIRNILNGTVFREPILCQ 180

Query: 181 NIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP 240
           NIPRIVPGWKKPICIGRHAFGDQ RATDTIIPGPGKLKMVFVPEN ETPRELEVYDFKGP
Sbjct: 181 NIPRIVPGWKKPICIGRHAFGDQYRATDTIIPGPGKLKMVFVPENGETPRELEVYDFKGP 240

Query: 241 GIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 300
           GI LTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK
Sbjct: 241 GIALTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 300

Query: 301 QKFEENLIWYEHWLIDDM-----------------------------------------L 307
           QKFEE  IWYEH LIDDM                                         L
Sbjct: 301 QKFEEYSIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLL 360

[Close]

BLAST of Carg02314 vs. NCBI nr
Match: XP_038890600.1 (isocitrate dehydrogenase [NADP] isoform X2 [Benincasa hispida])

HSP 1 Score: 492.3 bits (1266), Expect = 3.3e-135
Identity = 263/386 (68.13%), Postives = 276/386 (71.50%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60
           M  RL+RTAM SA+LFSSSV+LK+PSL  + +SSTLFNGRLLRN LPCP+RSPAVS RC 
Sbjct: 1   MIPRLVRTAMPSATLFSSSVSLKNPSLHLASSSSTLFNGRLLRNHLPCPLRSPAVSLRCC 60

Query: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120
           ASSS FDRVRV  PVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDAT+DR
Sbjct: 61  ASSSSFDRVRVLNPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATDDR 120

Query: 121 VTIESAEATLKY---------------------------------------CFPRTYLCQ 180
           VTIESAEATLKY                                        F    LCQ
Sbjct: 121 VTIESAEATLKYNVAVKCATITPDEARVKEFGLKSMWRSPNGTIRNILNGTVFREPILCQ 180

Query: 181 NIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP 240
           NIPRIVPGWKKPICIGRHAFGDQ RATDT+IPGPGKLKMVF+PEN E PRELEVYDFKGP
Sbjct: 181 NIPRIVPGWKKPICIGRHAFGDQYRATDTVIPGPGKLKMVFIPENGEAPRELEVYDFKGP 240

Query: 241 GIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 300
           GI LTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQ+VYEEKWK
Sbjct: 241 GIALTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQKVYEEKWK 300

Query: 301 QKFEENLIWYEHWLIDDM-----------------------------------------L 307
           QKFEEN IWYEH LIDDM                                         L
Sbjct: 301 QKFEENSIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLL 360

[Close]

BLAST of Carg02314 vs. ExPASy Swiss-Prot
Match: Q8LPJ5 (Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g14590 PE=1 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.2e-104
Identity = 201/381 (52.76%), Postives = 240/381 (62.99%), Query Frame = 0


Query: 7   RTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIR-SPAVSHRCYASSSG 66
           R ++++    S+S  L    +     SS +F+    RN++  P++   A + RC+ASS G
Sbjct: 13  RASLTAMLSSSTSAGLSSSFVSSRFLSSGIFSSGASRNRVTFPVQFHRASAVRCFASSGG 72

Query: 67  FDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDRVTIES 126
            DR++V  P+VEMDGDEMTR+IW+MIKEKLI P+LDLDIKY+DLGILNRDAT+D+VT+ES
Sbjct: 73  SDRIQVQNPIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVES 132

Query: 127 AEATLKY---------------------------------------CFPRTYLCQNIPRI 186
           AEA LKY                                        F    +C NIPR+
Sbjct: 133 AEAALKYNVAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRL 192

Query: 187 VPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGPGIGLT 246
           VPGW+KPICIGRHAFGDQ RATDT+I GPGKLKMVFVPE+   P EL+VYDFKGPG+ L 
Sbjct: 193 VPGWEKPICIGRHAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVALA 252

Query: 247 MYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWKQKFEE 306
           MYNVDESI+AFAESSM++A  KKWPLYLSTKNTILKKYDGRFKDIFQEVYE  WKQKFEE
Sbjct: 253 MYNVDESIRAFAESSMAMALTKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKQKFEE 312

[Close]

BLAST of Carg02314 vs. ExPASy Swiss-Prot
Match: Q40345 (Isocitrate dehydrogenase [NADP], chloroplastic (Fragment) OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.0e-87
Identity = 172/339 (50.74%), Postives = 207/339 (61.06%), Query Frame = 0


Query: 62  SSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDRV 121
           ++ GF +++V+ P+VEMDGDEMTRIIW  IK+KLIFPF++LDIKY+DLG+  RD TND+V
Sbjct: 20  ATMGFQKIKVANPIVEMDGDEMTRIIWKYIKDKLIFPFVELDIKYFDLGLPYRDETNDKV 79

Query: 122 TIESAEATLKY---------------------------------------CFPRTYLCQN 181
           T+ESAEATLKY                                        F    +C+N
Sbjct: 80  TVESAEATLKYNVAIKCATITPDEARVKEFGLKSMWRSPNGTIRNILNGTVFREPIICKN 139

Query: 182 IPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP- 241
           IPR++PGW KPICIGRHAFGDQ RATD++I GPGKLK+VFVPE      +LEVY+F G  
Sbjct: 140 IPRLIPGWTKPICIGRHAFGDQYRATDSVIKGPGKLKLVFVPEGQGETTDLEVYNFTGEG 199

Query: 242 GIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 301
           G+ L MYN DESI++FAE+SM++A EKKWPLYLSTKNTILKKYDGRFKDIFQEVYE  WK
Sbjct: 200 GVALAMYNTDESIRSFAEASMAVALEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEAGWK 259

Query: 302 QKFEENLIWYEHWLIDDMLS---------------------------------------- 320
            K+E   IWYEH LIDDM++                                        
Sbjct: 260 SKYEAAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLV 319

[Close]

BLAST of Carg02314 vs. ExPASy Swiss-Prot
Match: P50218 (Isocitrate dehydrogenase [NADP] OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.9e-87
Identity = 170/335 (50.75%), Postives = 205/335 (61.19%), Query Frame = 0


Query: 66  FDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDRVTIES 125
           FD+++V  P+VEMDGDEMTR+IW  IK+KLI PFL+LDIKY+DLG+ +RDAT+D+VT+ES
Sbjct: 3   FDKIKVENPIVEMDGDEMTRVIWKSIKDKLICPFLELDIKYFDLGLPHRDATDDKVTVES 62

Query: 126 AEATLKY---------------------------------------CFPRTYLCQNIPRI 185
           AEAT KY                                        F    +C+NIPR+
Sbjct: 63  AEATQKYNVAIKCATITPDEARVKEFNLKSMWRSPNGTIRNILNGTVFREPIMCKNIPRL 122

Query: 186 VPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP-GIGL 245
           VPGW KPICIGRHAFGDQ RATDT+I G GKLK+VFVPE  +   E EVY+F G  G+ L
Sbjct: 123 VPGWTKPICIGRHAFGDQYRATDTVIQGAGKLKLVFVPEGTDEKTEFEVYNFTGAGGVAL 182

Query: 246 TMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWKQKFE 305
           +MYN DES+++FAE+SM++A++KKWPLYLSTKNTILKKYDGRFKDIFQEVYE  WK K+E
Sbjct: 183 SMYNTDESVRSFAEASMNMAYQKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKYE 242

Query: 306 ENLIWYEHWLIDDM-----------------------------------------LSSDG 320
           E  IWYEH LIDDM                                         +  DG
Sbjct: 243 EAGIWYEHRLIDDMAAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDG 302

[Close]

BLAST of Carg02314 vs. ExPASy Swiss-Prot
Match: P50217 (Isocitrate dehydrogenase [NADP] OS=Solanum tuberosum OX=4113 GN=ICDH-1 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.1e-85
Identity = 169/335 (50.45%), Postives = 203/335 (60.60%), Query Frame = 0


Query: 66  FDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDRVTIES 125
           F ++ V  P+VEMDGDEMTR+IW  IK+KLI PFL+LDIKY+ LG+ +RDAT+D+VT+ES
Sbjct: 3   FQKITVQNPIVEMDGDEMTRVIWKSIKDKLILPFLELDIKYFSLGLPHRDATDDKVTVES 62

Query: 126 AEATLKY---------------------------------------CFPRTYLCQNIPRI 185
           AEAT KY                                        F    +C+NIPR+
Sbjct: 63  AEATQKYNVAIKCATITPDEARVKEFNLKSMWRSPNGTIRNILNGTVFREPIMCKNIPRL 122

Query: 186 VPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP-GIGL 245
           VPGW KPICIGRHAFGDQ RATDT+I G GKLK+VFVPE  +   E EVY+F G  G+ L
Sbjct: 123 VPGWTKPICIGRHAFGDQYRATDTVIKGAGKLKLVFVPEGSDEKTEFEVYNFTGAGGVAL 182

Query: 246 TMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWKQKFE 305
           +MYN DES+++FAE+SM++AF+KKWPLYLSTKNTILKKYDGRFKDIFQEVYE  WK K+E
Sbjct: 183 SMYNTDESVRSFAEASMNMAFQKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKYE 242

Query: 306 ENLIWYEHWLIDDMLS-----------------------------------------SDG 320
           E  IWYEH LIDDM++                                          DG
Sbjct: 243 EAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDG 302

[Close]

BLAST of Carg02314 vs. ExPASy Swiss-Prot
Match: Q06197 (Isocitrate dehydrogenase [NADP] OS=Glycine max OX=3847 GN=IDH1 PE=1 SV=2)

HSP 1 Score: 314.3 bits (804), Expect = 1.6e-84
Identity = 172/337 (51.04%), Postives = 200/337 (59.35%), Query Frame = 0


Query: 64  SGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDRVTI 123
           + F +++V+ P+VEMDGDEMTR+IW  IK+KLI PFL+LDIKYYDLG+  RD T+D+VTI
Sbjct: 2   AAFQKIKVANPIVEMDGDEMTRVIWKSIKDKLILPFLELDIKYYDLGLPYRDETDDKVTI 61

Query: 124 ESAEATLKY---------------------------------------CFPRTYLCQNIP 183
           ESAEATLKY                                        F    LC+NIP
Sbjct: 62  ESAEATLKYNVAIKCATITPDEARVKEFGLKSMWKSPNGTIRNILNGTVFREPILCKNIP 121

Query: 184 RIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP-GI 243
           R+VPGW K ICIGRHAFGDQ RATDT+I G GKLK+VFVPE      E EV++F G  G+
Sbjct: 122 RLVPGWTKAICIGRHAFGDQYRATDTVIKGAGKLKLVFVPEGQGEETEFEVFNFTGEGGV 181

Query: 244 GLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWKQK 303
            L MYN DESI++FAE+SM+ A EKKWPLYLSTKNTILKKYDGRFKDIFQEVYE  WK K
Sbjct: 182 SLAMYNTDESIRSFAEASMATALEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSK 241

Query: 304 FEENLIWYEHWLIDDMLS-----------------------------------------S 320
           FE   IWYEH LIDDM++                                          
Sbjct: 242 FEAAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCP 301

[Close]

BLAST of Carg02314 vs. ExPASy TrEMBL
Match: A0A6J1HH92 (Isocitrate dehydrogenase [NADP] OS=Cucurbita moschata OX=3662 GN=LOC111463463 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 6.6e-137
Identity = 268/386 (69.43%), Postives = 276/386 (71.50%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60
           MKARLIRTAMSSA+L SSS+ALK+P   FS T ST FNGRLL+NQLPCPIR PAVS RCY
Sbjct: 1   MKARLIRTAMSSATLLSSSLALKNPCFHFSSTFSTPFNGRLLKNQLPCPIRFPAVSLRCY 60

Query: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120
           ASSSG DRVRV  PVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDAT+DR
Sbjct: 61  ASSSGVDRVRVLNPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATDDR 120

Query: 121 VTIESAEATLKY---------------------------------------CFPRTYLCQ 180
           VT+ESAEATLKY                                        F    LCQ
Sbjct: 121 VTVESAEATLKYNVAVKCATITPDEARVKEFGLKAMWRSPNGTIRNILNGTVFREPILCQ 180

Query: 181 NIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP 240
           NIPRIVPGWKKPICIGRHAFGDQ RATDTIIPGPGKLKMVFVPEN ETPRELEVYDFKGP
Sbjct: 181 NIPRIVPGWKKPICIGRHAFGDQYRATDTIIPGPGKLKMVFVPENGETPRELEVYDFKGP 240

Query: 241 GIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 300
           GI LTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK
Sbjct: 241 GIALTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 300

Query: 301 QKFEENLIWYEHWLIDDM-----------------------------------------L 307
           QKFEE  IWYEH LIDDM                                         L
Sbjct: 301 QKFEEYSIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLL 360

[Close]

BLAST of Carg02314 vs. ExPASy TrEMBL
Match: A0A6J1JP31 (Isocitrate dehydrogenase [NADP] OS=Cucurbita maxima OX=3661 GN=LOC111487611 PE=3 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.1e-135
Identity = 265/386 (68.65%), Postives = 275/386 (71.24%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60
           MK RLIRTAMSSA+L SSS+ALK+PS  FS T ST FNGRLL+NQLPCPIR PAVS RCY
Sbjct: 1   MKGRLIRTAMSSATLLSSSLALKNPSFHFSSTFSTPFNGRLLKNQLPCPIRLPAVSLRCY 60

Query: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120
           ASSSG DRVRV  PVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDAT+DR
Sbjct: 61  ASSSGVDRVRVLNPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATDDR 120

Query: 121 VTIESAEATLKY---------------------------------------CFPRTYLCQ 180
           VT+ESAEATLKY                                        F    LCQ
Sbjct: 121 VTVESAEATLKYNVAVKCATITPDEARVKEFGLKAMWRSPNGTIRNILNGTVFREPILCQ 180

Query: 181 NIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP 240
           NIPRIVPGWKKPICIGRHAFGDQ RATDTIIPGPGKLKMVFVPE+ ETPRELEVYDFKGP
Sbjct: 181 NIPRIVPGWKKPICIGRHAFGDQYRATDTIIPGPGKLKMVFVPEDGETPRELEVYDFKGP 240

Query: 241 GIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 300
           GI L MYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQ+VYEEKWK
Sbjct: 241 GIALAMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQKVYEEKWK 300

Query: 301 QKFEENLIWYEHWLIDDM-----------------------------------------L 307
           QKFEE  IWYEH LIDDM                                         L
Sbjct: 301 QKFEEYSIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLL 360

[Close]

BLAST of Carg02314 vs. ExPASy TrEMBL
Match: A0A6J1C1Y5 (Isocitrate dehydrogenase [NADP] OS=Momordica charantia OX=3673 GN=LOC111006675 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.4e-133
Identity = 260/386 (67.36%), Postives = 275/386 (71.24%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIRSPAVSHRCY 60
           M+ARL+R+AM SA+LFSS VA K+PSL FS TSS+LFNGRLLRNQL CPIRSPAVS RCY
Sbjct: 1   MRARLLRSAMPSATLFSSPVAPKNPSLPFSPTSSSLFNGRLLRNQLSCPIRSPAVSLRCY 60

Query: 61  ASSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDR 120
           ASSSGFDRVRV  P+VEMDGDEMTRIIW MIKEKLIFP+LDLDIKY+DLGILNRDAT+DR
Sbjct: 61  ASSSGFDRVRVLNPIVEMDGDEMTRIIWAMIKEKLIFPYLDLDIKYFDLGILNRDATDDR 120

Query: 121 VTIESAEATLKY---------------------------------------CFPRTYLCQ 180
           VT ESAEATLKY                                        F    LCQ
Sbjct: 121 VTTESAEATLKYNVAVKCATITPDEARVKEFGLKAMWRSPNGTIRNILNGTVFREPILCQ 180

Query: 181 NIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP 240
           NIPRIVPGWKKPICIGRHAFGDQ RATDTIIPGPGKLKMV+VPEN ETP EL+VYDFKGP
Sbjct: 181 NIPRIVPGWKKPICIGRHAFGDQYRATDTIIPGPGKLKMVYVPENGETPTELDVYDFKGP 240

Query: 241 GIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWK 300
           GI LTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQ+VYEEKWK
Sbjct: 241 GIALTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQKVYEEKWK 300

Query: 301 QKFEENLIWYEHWLIDDM-----------------------------------------L 307
           QKFE   IWYEH LIDDM                                         L
Sbjct: 301 QKFEGKSIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLL 360

[Close]

BLAST of Carg02314 vs. ExPASy TrEMBL
Match: A0A1S3C8N1 (Isocitrate dehydrogenase [NADP] OS=Cucumis melo OX=3656 GN=LOC103498090 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.7e-124
Identity = 254/388 (65.46%), Postives = 266/388 (68.56%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLTS-STLFNGRLLRNQLPCPIRSPAVSHRC 60
           M  RLIRT MS+ +LFSS  ALK+PS RFS TS STLFNG      L  P+RS AVS RC
Sbjct: 1   MMPRLIRTTMSTTTLFSSPFALKNPSSRFSSTSTSTLFNGTRF---LTSPLRSHAVSLRC 60

Query: 61  YASS-SGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATN 120
           YASS S FDRV V  PVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDAT+
Sbjct: 61  YASSPSAFDRVPVLNPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATD 120

Query: 121 DRVTIESAEATLKY---------------------------------------CFPRTYL 180
           DRVT+ESAEATLKY                                        F    L
Sbjct: 121 DRVTVESAEATLKYNVAVKCATITPDEARVKEFGLKTMWRSPNGTIRNILNGTVFREPIL 180

Query: 181 CQNIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFK 240
           C+NIPRIVPGWKKPICIGRHAFGDQ RATDT+IPGPGKLKMVFVPEN ETPRELEVYDFK
Sbjct: 181 CENIPRIVPGWKKPICIGRHAFGDQYRATDTVIPGPGKLKMVFVPENGETPRELEVYDFK 240

Query: 241 GPGIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEK 300
           G GI LTMYNVD+SIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQ+VYEEK
Sbjct: 241 GSGIALTMYNVDKSIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQKVYEEK 300

Query: 301 WKQKFEENLIWYEHWLIDDM---------------------------------------- 307
           WKQKFEEN IWYEH LIDDM                                        
Sbjct: 301 WKQKFEENSIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSV 360

[Close]

BLAST of Carg02314 vs. ExPASy TrEMBL
Match: A0A0A0KP32 (Isocitrate dehydrogenase [NADP] OS=Cucumis sativus OX=3659 GN=Csa_5G523180 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 8.3e-124
Identity = 254/390 (65.13%), Postives = 266/390 (68.21%), Query Frame = 0


Query: 1   MKARLIRTAMSSASLFSSSVALKDPSLRFSLT-SSTLFNGRLLRNQLPCPIRSPAVSHRC 60
           M  RLIRT MSSA+LFSSS+ LK+PS  FS T SSTLFNG      L  P+RSPA+S RC
Sbjct: 1   MMPRLIRTTMSSATLFSSSLPLKNPSFPFSSTSSSTLFNGPRF---LTSPLRSPALSLRC 60

Query: 61  YA---SSSGFDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDA 120
           YA   SSS  DRV V  PVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKY+DLGILNRDA
Sbjct: 61  YASSSSSSSSDRVPVLNPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYFDLGILNRDA 120

Query: 121 TNDRVTIESAEATLKY---------------------------------------CFPRT 180
           T+DRVT+ESAEATLKY                                        F   
Sbjct: 121 TDDRVTVESAEATLKYNVAVKCATITPDEARVKEFGLKAMWRSPNGTIRNILNGTVFREP 180

Query: 181 YLCQNIPRIVPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYD 240
            LCQNIPRIVPGWKKPICIGRHAFGDQ RATDT+IPGPGKLKMVFVPEN ETPRELEVYD
Sbjct: 181 ILCQNIPRIVPGWKKPICIGRHAFGDQYRATDTVIPGPGKLKMVFVPENGETPRELEVYD 240

Query: 241 FKGPGIGLTMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYE 300
           FKG GI L MYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQ+VYE
Sbjct: 241 FKGSGIALAMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQKVYE 300

Query: 301 EKWKQKFEENLIWYEHWLIDDM-------------------------------------- 307
           EKWKQKFEEN IWYEH LIDDM                                      
Sbjct: 301 EKWKQKFEENSIWYEHRLIDDMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMT 360

[Close]

BLAST of Carg02314 vs. TAIR 10
Match: AT5G14590.1 (Isocitrate/isopropylmalate dehydrogenase family protein )

HSP 1 Score: 378.3 bits (970), Expect = 6.6e-105
Identity = 201/381 (52.76%), Postives = 240/381 (62.99%), Query Frame = 0


Query: 7   RTAMSSASLFSSSVALKDPSLRFSLTSSTLFNGRLLRNQLPCPIR-SPAVSHRCYASSSG 66
           R ++++    S+S  L    +     SS +F+    RN++  P++   A + RC+ASS G
Sbjct: 13  RASLTAMLSSSTSAGLSSSFVSSRFLSSGIFSSGASRNRVTFPVQFHRASAVRCFASSGG 72

Query: 67  FDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDRVTIES 126
            DR++V  P+VEMDGDEMTR+IW+MIKEKLI P+LDLDIKY+DLGILNRDAT+D+VT+ES
Sbjct: 73  SDRIQVQNPIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVES 132

Query: 127 AEATLKY---------------------------------------CFPRTYLCQNIPRI 186
           AEA LKY                                        F    +C NIPR+
Sbjct: 133 AEAALKYNVAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRL 192

Query: 187 VPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGPGIGLT 246
           VPGW+KPICIGRHAFGDQ RATDT+I GPGKLKMVFVPE+   P EL+VYDFKGPG+ L 
Sbjct: 193 VPGWEKPICIGRHAFGDQYRATDTVIKGPGKLKMVFVPEDGNAPVELDVYDFKGPGVALA 252

Query: 247 MYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWKQKFEE 306
           MYNVDESI+AFAESSM++A  KKWPLYLSTKNTILKKYDGRFKDIFQEVYE  WKQKFEE
Sbjct: 253 MYNVDESIRAFAESSMAMALTKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKQKFEE 312

[Close]

BLAST of Carg02314 vs. TAIR 10
Match: AT1G54340.1 (isocitrate dehydrogenase )

HSP 1 Score: 313.2 bits (801), Expect = 2.6e-85
Identity = 168/334 (50.30%), Postives = 198/334 (59.28%), Query Frame = 0


Query: 66  FDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDRVTIES 125
           F++++V  PVVEMDGDEMTR+IW  IK+KLIFPFL+LDIKY+DLG+ NRD T+D+VTIE+
Sbjct: 3   FEKIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIET 62

Query: 126 AEATLKY---------------------------------------CFPRTYLCQNIPRI 185
           AEATLKY                                        F    +C+NIPR+
Sbjct: 63  AEATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRL 122

Query: 186 VPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGPGIGLT 245
           VPGW KPICIGRHAFGDQ RATD I+  PGKLK+VF P       E EV++F G G+ L 
Sbjct: 123 VPGWTKPICIGRHAFGDQYRATDLIVNEPGKLKLVFEPSGSSQKTEFEVFNFTGGGVALA 182

Query: 246 MYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWKQKFEE 305
           MYN DESI+AFAESSM  A++KKWPLYLSTKNTILK YDGRFKDIFQEVYE  W+ K+E 
Sbjct: 183 MYNTDESIRAFAESSMYTAYQKKWPLYLSTKNTILKIYDGRFKDIFQEVYEANWRSKYEA 242

Query: 306 NLIWYEHWLIDDMLS-----------------------------------------SDGK 320
             IWYEH LIDDM++                                          DGK
Sbjct: 243 AGIWYEHRLIDDMVAYAMKSEGGYVWACKNYDGDVQSDFLAQGYGSLGMMTSVLVCPDGK 302

[Close]

BLAST of Carg02314 vs. TAIR 10
Match: AT1G65930.1 (cytosolic NADP+-dependent isocitrate dehydrogenase )

HSP 1 Score: 300.1 bits (767), Expect = 2.3e-81
Identity = 162/335 (48.36%), Postives = 199/335 (59.40%), Query Frame = 0


Query: 66  FDRVRVSGPVVEMDGDEMTRIIWTMIKEKLIFPFLDLDIKYYDLGILNRDATNDRVTIES 125
           F++++V+ P+VEMDGDEMTR+IW  IK+KLI PF++LDIKY+DLG+ +RDAT+D+VTIES
Sbjct: 3   FEKIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIES 62

Query: 126 AEATLKY---------------------------------------CFPRTYLCQNIPRI 185
           AEAT KY                                        F    +C+N+P++
Sbjct: 63  AEATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKL 122

Query: 186 VPGWKKPICIGRHAFGDQNRATDTIIPGPGKLKMVFVPENVETPRELEVYDFKGP-GIGL 245
           VPGW KPICIGRHAFGDQ RATD +I GPGKL M F  E  +   E EV+ F G  G+ +
Sbjct: 123 VPGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTF--EGKDGKTETEVFTFTGEGGVAM 182

Query: 246 TMYNVDESIQAFAESSMSLAFEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEEKWKQKFE 305
            MYN DESI+AFA++SM+ A+EKKWPLYLSTKNTILKKYDGRFKDIFQEVYE  WK K++
Sbjct: 183 AMYNTDESIRAFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYD 242

Query: 306 ENLIWYEHWLIDDMLS-----------------------------------------SDG 320
              IWYEH LIDDM++                                          DG
Sbjct: 243 AAGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDG 302

The following BLAST results are available for this feature:

BLAST of Carg02314 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016201.1	3.0e-192	100.00	Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial, partial [Cucurbita...	[more]
KAG6578661.1	2.7e-153	100.00	Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial, partial [Cucurbita...	[more]
XP_023515364.1	1.4e-136	69.43	isocitrate dehydrogenase [NADP] [Cucurbita pepo subsp. pepo]	[more]
XP_022963180.1	1.4e-136	69.43	isocitrate dehydrogenase [NADP]-like [Cucurbita moschata]	[more]
XP_038890600.1	3.3e-135	68.13	isocitrate dehydrogenase [NADP] isoform X2 [Benincasa hispida]	[more]

BLAST of Carg02314 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPJ5	9.2e-104	52.76	Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial OS=Arabidopsis thal...	[more]
Q40345	6.0e-87	50.74	Isocitrate dehydrogenase [NADP], chloroplastic (Fragment) OS=Medicago sativa OX=...	[more]
P50218	7.9e-87	50.75	Isocitrate dehydrogenase [NADP] OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P50217	1.1e-85	50.45	Isocitrate dehydrogenase [NADP] OS=Solanum tuberosum OX=4113 GN=ICDH-1 PE=2 SV=1	[more]
Q06197	1.6e-84	51.04	Isocitrate dehydrogenase [NADP] OS=Glycine max OX=3847 GN=IDH1 PE=1 SV=2	[more]

BLAST of Carg02314 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HH92	6.6e-137	69.43	Isocitrate dehydrogenase [NADP] OS=Cucurbita moschata OX=3662 GN=LOC111463463 PE...	[more]
A0A6J1JP31	2.1e-135	68.65	Isocitrate dehydrogenase [NADP] OS=Cucurbita maxima OX=3661 GN=LOC111487611 PE=3...	[more]
A0A6J1C1Y5	4.4e-133	67.36	Isocitrate dehydrogenase [NADP] OS=Momordica charantia OX=3673 GN=LOC111006675 P...	[more]
A0A1S3C8N1	3.7e-124	65.46	Isocitrate dehydrogenase [NADP] OS=Cucumis melo OX=3656 GN=LOC103498090 PE=3 SV=...	[more]
A0A0A0KP32	8.3e-124	65.13	Isocitrate dehydrogenase [NADP] OS=Cucumis sativus OX=3659 GN=Csa_5G523180 PE=3 ...	[more]

BLAST of Carg02314 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14590.1	6.6e-105	52.76	Isocitrate/isopropylmalate dehydrogenase family protein	[more]
AT1G54340.1	2.6e-85	50.30	isocitrate dehydrogenase	[more]
AT1G65930.1	2.3e-81	48.36	cytosolic NADP+-dependent isocitrate dehydrogenase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024084	Isopropylmalate dehydrogenase-like domain	SMART	SM01329	Iso_dh_2	coord: 74..331 e-value: 3.2E-11 score: -37.7
IPR024084	Isopropylmalate dehydrogenase-like domain	PFAM	PF00180	Iso_dh	coord: 74..132 e-value: 2.1E-5 score: 23.9 coord: 139..280 e-value: 1.7E-16 score: 60.3
None	No IPR available	GENE3D	3.40.718.10	Isopropylmalate Dehydrogenase	coord: 67..282 e-value: 2.3E-79 score: 268.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 313..332
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 308..332
None	No IPR available	PANTHER	PTHR11822:SF5	ISOCITRATE DEHYDROGENASE [NADP], CHLOROPLASTIC/MITOCHONDRIAL	coord: 54..133 coord: 134..280
None	No IPR available	SUPERFAMILY	53659	Isocitrate/Isopropylmalate dehydrogenase-like	coord: 67..296
IPR004790	Isocitrate dehydrogenase NADP-dependent	PANTHER	PTHR11822	NADP-SPECIFIC ISOCITRATE DEHYDROGENASE	coord: 54..133 coord: 134..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02314-RA	Carg02314-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006102	isocitrate metabolic process
cellular_component	GO:0043229	intracellular organelle
molecular_function	GO:0004450	isocitrate dehydrogenase (NADP+) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor