Carg02290 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg02290 vs. NCBI nr
Match: KAG7016178.1 (Folylpolyglutamate synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1035.0 bits (2675), Expect = 2.2e-298
Identity = 517/517 (100.00%), Postives = 517/517 (100.00%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV
Sbjct: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC
Sbjct: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG
Sbjct: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH
Sbjct: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360

Query: 361 QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420
           QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV
Sbjct: 361 QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420

Query: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480
           FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM
Sbjct: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480

Query: 481 TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
           TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 517

BLAST of Carg02290 vs. NCBI nr
Match: XP_022939781.1 (folylpolyglutamate synthase-like [Cucurbita moschata] >XP_022939782.1 folylpolyglutamate synthase-like [Cucurbita moschata])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.3e-292
Identity = 505/517 (97.68%), Postives = 512/517 (99.03%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           MLEGNDTLMHS+TAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV
Sbjct: 1   MLEGNDTLMHSQTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDG+NISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGINISEEKFLTYFWW 120

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYNKLKE+TSKDIPMPHLFRFLALLA KIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC
Sbjct: 121 CYNKLKEKTSKDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFT+FQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTLFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG
Sbjct: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LTSASFDGRAQIVADR + IESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGML+H
Sbjct: 301 LTSASFDGRAQIVADRCVDIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLNH 360

Query: 361 QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420
           QSEDKSTSDN+EARKKSVQVLLFNCMTAR PQLLLPPLIKTCANNGVHFKKALFVPNTSV
Sbjct: 361 QSEDKSTSDNNEARKKSVQVLLFNCMTARDPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420

Query: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480
           FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM
Sbjct: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480

Query: 481 TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
            IKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 AIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 517

BLAST of Carg02290 vs. NCBI nr
Match: XP_022993583.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.1e-291
Identity = 506/517 (97.87%), Postives = 509/517 (98.45%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV
Sbjct: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYNKLKE+TSKDIPMPHLFRFLALLA KIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC
Sbjct: 121 CYNKLKEKTSKDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELLHG KLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPE+FIEG
Sbjct: 241 VPSLDPELLHGCKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEQFIEG 300

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LTSASFDGRAQIVADRYI IESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGML+H
Sbjct: 301 LTSASFDGRAQIVADRYISIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLNH 360

Query: 361 QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420
           QSEDKS S NDEARKKSVQVLLFNCMTAR PQLLLPPL KTCANNGVHFKKALFVPNTSV
Sbjct: 361 QSEDKSASYNDEARKKSVQVLLFNCMTARDPQLLLPPLFKTCANNGVHFKKALFVPNTSV 420

Query: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480
           FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM
Sbjct: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480

Query: 481 TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
            IKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 AIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 517

BLAST of Carg02290 vs. NCBI nr
Match: XP_023550825.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.0e-291
Identity = 504/517 (97.49%), Postives = 507/517 (98.07%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           MLEGNDTLMHSRT YEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV
Sbjct: 1   MLEGNDTLMHSRTTYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYNKLKE+TSKDIPMPHLFRFLALLA KIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC
Sbjct: 121 CYNKLKEKTSKDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG
Sbjct: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LTSASFDGRAQIVADRY GIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGML+H
Sbjct: 301 LTSASFDGRAQIVADRYNGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLNH 360

Query: 361 QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420
           QSEDKSTSD DEARKKSVQVLLFNCM+AR PQ LLPPLIKTC NNGVHFKKALFVPNTSV
Sbjct: 361 QSEDKSTSDKDEARKKSVQVLLFNCMSARAPQRLLPPLIKTCVNNGVHFKKALFVPNTSV 420

Query: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480
           FNKVGSHSL EANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM
Sbjct: 421 FNKVGSHSLTEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480

Query: 481 TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
            IKWLRDSV QQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 AIKWLRDSVSQQNRSIRLQVLVTGSLHLVGDVLKLIK 517

BLAST of Carg02290 vs. NCBI nr
Match: XP_022993584.1 (folylpolyglutamate synthase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1006.9 bits (2602), Expect = 6.3e-290
Identity = 505/517 (97.68%), Postives = 508/517 (98.26%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV
Sbjct: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYNKLKE+TSKDIPMPHLFRFLALLA KIFSDEQVDVAILEVGLGGTFDATN VQEPLVC
Sbjct: 121 CYNKLKEKTSKDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATN-VQEPLVC 180

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELLHG KLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPE+FIEG
Sbjct: 241 VPSLDPELLHGCKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEQFIEG 300

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LTSASFDGRAQIVADRYI IESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGML+H
Sbjct: 301 LTSASFDGRAQIVADRYISIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLNH 360

Query: 361 QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420
           QSEDKS S NDEARKKSVQVLLFNCMTAR PQLLLPPL KTCANNGVHFKKALFVPNTSV
Sbjct: 361 QSEDKSASYNDEARKKSVQVLLFNCMTARDPQLLLPPLFKTCANNGVHFKKALFVPNTSV 420

Query: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480
           FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM
Sbjct: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480

Query: 481 TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
            IKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 AIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 516

BLAST of Carg02290 vs. ExPASy Swiss-Prot
Match: F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 3.0e-178
Identity = 323/509 (63.46%), Postives = 394/509 (77.41%), Query Frame = 0

Query: 14  AYEEALSVLSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEEPMRRMKVIHVAGTKGKGS 73
           +YEEAL+ LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + +M VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLKERTSK 133
           TCTFTESI+R+ GF TGLFTSPHLID+RERFRLDGV+ISEEKFL YFWWCYN+LKERT++
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIASLGYDHME 193
           +IPMP  FRFLALLA KIF+ E+VD AILEVGLGG FDATN VQ+P+VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 ILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELLHG 253
           ILG+TL +IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 RKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEGLTSASFDGRAQ 313
           +KLGL+GEHQY+NAGLA++L+S WL + G  ++    Q   LPEKFI+GL +AS  GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIR-HNQRGMLSHQSEDKSTSDN 373
           +V D+Y    +   LVFYLDGAHSPESME CA+WFS A++  NQ G   H     + S +
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 374 DE-ARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSVFNKVGSHS- 433
           D+ + +   Q+LLFNCM+ R P LLLP L   CA  GV+FKKALFVPN SV++KVG+ + 
Sbjct: 426 DKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHKVGTAAD 485

Query: 434 LAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPMTIKWLRDS 493
           L E + QVDLSWQF +++VW++ +Q    S+   E  G+S  NS VF+SLPM IK LRD+
Sbjct: 486 LPENDPQVDLSWQFTLQKVWESLVQ----SERDGEKDGESDGNSEVFTSLPMAIKCLRDT 545

Query: 494 VLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
           V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 VHESSSATRFQVLVTGSLHLVGDVLRLIR 570

BLAST of Carg02290 vs. ExPASy Swiss-Prot
Match: Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 4.9e-160
Identity = 293/505 (58.02%), Postives = 368/505 (72.87%), Query Frame = 0

Query: 15  YEEALSVLSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMRRMKVIHVAGTKGKGST 74
           Y+  L  LSSLI  R R   +    RF L+F Y+K+L+LE+ + +MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLKERTSKD 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+G+ IS+EKF+ YFW C++KLKE+TS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 IPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIASLGYDHMEI 194
           +PMP  F FLALLA KIF+ EQVDV ILEVGLGG FDATNV+Q+P+VCGI+SLGYDHMEI
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELLHGR 254
           LG TLAEIA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEGLTSASFDGRAQI 314
           +LGL+GEHQYLNAGLA+AL ST+L   G    + ++Q+  LPEKFI GL++A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSHQSEDKSTSDNDE 374
           V D     E PE +V+YLDGAHSPESME CA WFS  I+ NQ                + 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ----------------ER 372

Query: 375 ARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSVFNKVGSHSLAEA 434
            +K+S Q+LLFNCM+ R P LLLP L   C + GV FK+A+FVPN SV+N+VGS +    
Sbjct: 373 NQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVGSSTNVGT 432

Query: 435 NGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPMTIKWLRDSVLQQ 494
             +  +SWQF ++R+W++  + +  S+SK++  GK  E S VFSSLP+ + WLRD+  +Q
Sbjct: 433 RVE-SMSWQFGLQRIWESLARGEAKSNSKSDSKGKEEEKSFVFSSLPVAVDWLRDNA-RQ 491

Query: 495 NRSIRLQVLVTGSLHLVGDVLKLIK 518
           ++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SKQVRFQVLVTGSLHLVGDLLRFIK 491

BLAST of Carg02290 vs. ExPASy Swiss-Prot
Match: F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.3e-144
Identity = 275/545 (50.46%), Postives = 368/545 (67.52%), Query Frame = 0

Query: 13  TAYEEALSVLSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEEPMRRMKVIHVAGTK 72
           ++Y++A+  LS+LI  R R D+     + D+ E +  Y+K+LDLE+ ++ +KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 73  GKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLKE 132
           GKGSTC F+E+ILR+CGF TG+FTSPHLID+RERFR+DG++ISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 133 RTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIASLGY 192
           +    + MP LF+FL +LA KIF  E+VDVA++EVGLGG  D+TNV+Q+P+VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 193 DHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPE 252
           DHM+ILGNTLA+IA  KAGIFK   PAFTV Q  EAM VL+  A+ L+V L VV  L+P+
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320

Query: 253 LLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSR--SLPEKFIEGLTSAS 312
            L G  LGL G+HQ +NAGLA++LS  WL R G+ K  +  +S+   +P  F  GL +A 
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380

Query: 313 FDGRAQIV-------ADRYIGIESPEG-LVFYLDGAHSPESMEVCARWFSAAIRHNQRGM 372
             GRAQ+V        D    +E+P G L+FYLDGAHSPESME C RWFS+A+R ++   
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVRGDKSLS 440

Query: 373 LSHQSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPN 432
            +     +      +  + S Q+LLFNCM  R PQ+LLP L+ TCA++G HF +ALFVP+
Sbjct: 441 TAVNGYMRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTHFSRALFVPS 500

Query: 433 TSVFNKV--GSHSLAEANGQVDLSWQFHVRRVWQNTIQ-RDKGSDSKAE----------- 492
            S +NKV  G+ ++     + DL+WQF ++R+W+ +IQ  D G D   +           
Sbjct: 501 MSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKPDGITALPPHD 560

Query: 493 -----------VVGKSCENSTVFSSLPMTIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDV 518
                        G    +S V  SLP+TI WLRD V ++N S++L+VLVTGSLHLVGDV
Sbjct: 561 FLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLVTGSLHLVGDV 620

BLAST of Carg02290 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 5.9e-81
Identity = 206/569 (36.20%), Postives = 291/569 (51.14%), Query Frame = 0

Query: 15  YEEALSVLSSLIVNRRRCD----KSGD---RFELMFEYVKMLDLE-EPMRRMKVIHVAGT 74
           Y++A+  L++L  N    +    K GD   + E M  Y+    L+ E + ++ +IH+ GT
Sbjct: 42  YQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGT 101

Query: 75  KGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLK 134
           KGKGSTC FTE ILRS G  TG F+SPHL+ +RER R++G  IS E F  +FW  Y++L+
Sbjct: 102 KGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLE 161

Query: 135 ER--TSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIAS 194
           E    S  + MP  FRFL L+A  +F  E+VD+A+LEVG+GG +D TN++++P+VCGI S
Sbjct: 162 ETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITS 221

Query: 195 LGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSL 254
           LG DH  +LG+T+ +IA +K GIFK G PAFTV QPDE + VLRD+A Q+   L + P L
Sbjct: 222 LGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPL 281

Query: 255 DPELLHG--RKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSR-SLP------- 314
                 G    LGLEGEHQ  NA LA+ ++  WL + G+     ++ SR SLP       
Sbjct: 282 QALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAP 341

Query: 315 -----EKFIEGLTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAA 374
                 +  +GL    + GR Q++         P  L +YLDGAH+  SM+ C RWF  A
Sbjct: 342 VFQPTPRMQQGLRHTEWPGRTQLLR------RGP--LTWYLDGAHTTSSMQACVRWFRQA 401

Query: 375 IRHNQRGMLSHQSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHF 434
           +         H+ E   +          V+VLLFN    R    LL  L++ C      F
Sbjct: 402 L---------HRCERPDSG-------SEVRVLLFNSTGDRDSAALL-KLLQPC-----QF 461

Query: 435 KKALFVPNTSVFNKVGSHSLAEANGQVD------LSWQFHVRRVWQNTIQRDKGSDSKAE 494
             A+F PN +      +         +D      L+ Q H  R+ +  +  D  S    E
Sbjct: 462 DYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSPDPWSTPGQE 521

Query: 495 VVGKS------------CENSTVFSSLPMTIKWL---RDSVLQQ---------------- 518
             G +              +S VFS +   ++W+   RD + Q                 
Sbjct: 522 QDGPASLLLAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADSG 580

BLAST of Carg02290 vs. ExPASy Swiss-Prot
Match: Q05932 (Folylpolyglutamate synthase, mitochondrial OS=Homo sapiens OX=9606 GN=FPGS PE=1 SV=3)

HSP 1 Score: 299.7 bits (766), Expect = 6.5e-80
Identity = 203/568 (35.74%), Postives = 288/568 (50.70%), Query Frame = 0

Query: 15  YEEALSVLSSLIVN-------RRRCDKSGDRFELMFEYVKMLDLE-EPMRRMKVIHVAGT 74
           Y++A+ +L++L  N       +R+      + E M  Y+    L+ E + R+ +IHV GT
Sbjct: 45  YQDAVRMLNTLQTNAGYLEQVKRQRGDPQTQLEAMELYLARSGLQVEDLDRLNIIHVTGT 104

Query: 75  KGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLK 134
           KGKGSTC FTE ILRS G  TG F+SPHL+ +RER R++G  IS E F  YFW  Y++L+
Sbjct: 105 KGKGSTCAFTECILRSYGLKTGFFSSPHLVQVRERIRINGQPISPELFTKYFWRLYHRLE 164

Query: 135 E-RTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIASL 194
           E +    + MP  FRFL L+A  +F  E+VD+A++EVG+GG +D TN++++P+VCG++SL
Sbjct: 165 ETKDGSCVSMPPYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNIIRKPVVCGVSSL 224

Query: 195 GYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLD 254
           G DH  +LG+T+ +IA +K GIFK+G PAFTV QP+  + VLRD+A Q+   L + P L+
Sbjct: 225 GIDHTSLLGDTVEKIAWQKGGIFKQGVPAFTVLQPEGPLAVLRDRAQQISCPLYLCPMLE 284

Query: 255 PELLHG--RKLGLEGEHQYLNAGLAIALSSTWL-----HRAGHSKLSYMEQSRSLP---- 314
                G    LGLEGEHQ  NA LA+ L+  WL     H AG  K S       LP    
Sbjct: 285 ALEEGGPPLTLGLEGEHQRSNAALALQLAHCWLQRQDRHGAGEPKASRPGLLWQLPLAPV 344

Query: 315 ----EKFIEGLTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAI 374
                    GL +  + GR Q++         P  L +YLDGAH+  S + C RWF  A+
Sbjct: 345 FQPTSHMRLGLRNTEWPGRTQVLR------RGP--LTWYLDGAHTASSAQACVRWFRQAL 404

Query: 375 RHNQRGMLSHQSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFK 434
           +  +R                 +    V+VLLFN    R P  LL  L++ C      F 
Sbjct: 405 QGRER----------------PSGGPEVRVLLFNATGDRDPAALL-KLLQPC-----QFD 464

Query: 435 KALFVPNTSVFNKVGSHSLAEANGQVD------LSWQFHVRRVWQNTIQRDKGSDSKAEV 494
            A+F PN +  +  G+         +D      L  Q H   + +     D  S    E 
Sbjct: 465 YAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRCLEHQQHWNHLDEEQASPDLWSAPSPEP 524

Query: 495 VGKS------------CENSTVFSSLPMTIKWL---RDSVLQQ----------------- 518
            G +              +S VFS +   ++W+   RD + Q                  
Sbjct: 525 GGSASLLLAPHPPHTCSASSLVFSCISHALQWISQGRDPIFQPPSPPKGLLTHPVAHSGA 582

BLAST of Carg02290 vs. ExPASy TrEMBL
Match: A0A6J1FGU9 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111445560 PE=3 SV=1)

HSP 1 Score: 1015.0 bits (2623), Expect = 1.1e-292
Identity = 505/517 (97.68%), Postives = 512/517 (99.03%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           MLEGNDTLMHS+TAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV
Sbjct: 1   MLEGNDTLMHSQTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDG+NISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGINISEEKFLTYFWW 120

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYNKLKE+TSKDIPMPHLFRFLALLA KIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC
Sbjct: 121 CYNKLKEKTSKDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFT+FQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTLFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG
Sbjct: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LTSASFDGRAQIVADR + IESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGML+H
Sbjct: 301 LTSASFDGRAQIVADRCVDIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLNH 360

Query: 361 QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420
           QSEDKSTSDN+EARKKSVQVLLFNCMTAR PQLLLPPLIKTCANNGVHFKKALFVPNTSV
Sbjct: 361 QSEDKSTSDNNEARKKSVQVLLFNCMTARDPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420

Query: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480
           FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM
Sbjct: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480

Query: 481 TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
            IKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 AIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 517

BLAST of Carg02290 vs. ExPASy TrEMBL
Match: A0A6J1K2P2 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111489534 PE=3 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 5.6e-292
Identity = 506/517 (97.87%), Postives = 509/517 (98.45%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV
Sbjct: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYNKLKE+TSKDIPMPHLFRFLALLA KIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC
Sbjct: 121 CYNKLKEKTSKDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELLHG KLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPE+FIEG
Sbjct: 241 VPSLDPELLHGCKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEQFIEG 300

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LTSASFDGRAQIVADRYI IESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGML+H
Sbjct: 301 LTSASFDGRAQIVADRYISIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLNH 360

Query: 361 QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420
           QSEDKS S NDEARKKSVQVLLFNCMTAR PQLLLPPL KTCANNGVHFKKALFVPNTSV
Sbjct: 361 QSEDKSASYNDEARKKSVQVLLFNCMTARDPQLLLPPLFKTCANNGVHFKKALFVPNTSV 420

Query: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480
           FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM
Sbjct: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480

Query: 481 TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
            IKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 AIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 517

BLAST of Carg02290 vs. ExPASy TrEMBL
Match: A0A6J1K0K7 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111489534 PE=3 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.0e-290
Identity = 505/517 (97.68%), Postives = 508/517 (98.26%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV
Sbjct: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYNKLKE+TSKDIPMPHLFRFLALLA KIFSDEQVDVAILEVGLGGTFDATN VQEPLVC
Sbjct: 121 CYNKLKEKTSKDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATN-VQEPLVC 180

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELLHG KLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPE+FIEG
Sbjct: 241 VPSLDPELLHGCKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEQFIEG 300

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LTSASFDGRAQIVADRYI IESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGML+H
Sbjct: 301 LTSASFDGRAQIVADRYISIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLNH 360

Query: 361 QSEDKSTSDNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSV 420
           QSEDKS S NDEARKKSVQVLLFNCMTAR PQLLLPPL KTCANNGVHFKKALFVPNTSV
Sbjct: 361 QSEDKSASYNDEARKKSVQVLLFNCMTARDPQLLLPPLFKTCANNGVHFKKALFVPNTSV 420

Query: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480
           FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM
Sbjct: 421 FNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPM 480

Query: 481 TIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
            IKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 AIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 516

BLAST of Carg02290 vs. ExPASy TrEMBL
Match: A0A0A0KTX7 (Folylpolyglutamate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G630860 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.2e-255
Identity = 445/538 (82.71%), Postives = 480/538 (89.22%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           M+EGN+TL  S+ AYEEAL  LSSLIVNRRRCDKSGD+FELMFEYVKMLDLEE M +MKV
Sbjct: 16  MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKV 75

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR+CGF TGLFTSPHLIDIRERFRLDG++ISEEKFLTYFW 
Sbjct: 76  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 135

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYN+LKERTS+DIPMPHLFRFLALLA KIFSDEQVDVAILEVGLGGTFDATNVVQ P+VC
Sbjct: 136 CYNRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 195

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDHME+LGNTL EIASEKAGIFKRGAPAFTVFQPDEAM VL+DKASQLDVQLTV
Sbjct: 196 GIASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTV 255

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELLHGRKLGLEGEHQYLNAGLA+ALSSTWLHR+GHS+LSY+EQSRSLPE+FIEG
Sbjct: 256 VPSLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEG 315

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LT+ASF+GRAQIV DRY  +E P+ LVFYLDGAHSPESME C RWFSAA+RHNQ G L+H
Sbjct: 316 LTTASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNH 375

Query: 361 QSEDKSTS---------DNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKK 420
           QSED  +          DNDEARKKSVQVLLFNCM  R PQLLLPPLIKTCANNGV+FKK
Sbjct: 376 QSEDNGSGTSSNKLQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFKK 435

Query: 421 ALFVPNTSVFNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDS----------- 480
           ALFVPNTSVFNKVG+H L E + QVDLSWQFHVRR+W++T Q DKGSDS           
Sbjct: 436 ALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDKGSDSKGTGAVLEEES 495

Query: 481 -KAEVVGKSCENSTVFSSLPMTIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
            K+EV GK+C NSTVFSSLP+ IKWLRDSVLQQNRS+RLQVLVTGSLHLVGDVLKLIK
Sbjct: 496 EKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 553

BLAST of Carg02290 vs. ExPASy TrEMBL
Match: A0A6J1JQH1 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 5.1e-253
Identity = 445/537 (82.87%), Postives = 470/537 (87.52%), Query Frame = 0

Query: 1   MLEGNDTLMHSRTAYEEALSVLSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMRRMKV 60
           M EG   L +SRTAYEEAL VL SLIVNRRRCDKSGDRFELMFEYVKMLDLEE M +MKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDG++ISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNKLKERTSKDIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVC 180
           CYN+LKE+TS+D+PMPHLFRFLALLA KIFSDEQVDVAILEVGLGGTFDATNVVQ P+VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMEILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240
           GIASLGYDH E+LGNTL  IASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEG 300
           VPSLDPELL G KLGLEGEHQYLNA LAIALSSTWLHR G S LSY EQS SLPE+FIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTSASFDGRAQIVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSH 360
           LT+A F+GRAQIV DRY  IE+PE LVFYLDGAHSPESM VC+ WF AAI+HNQ G+L+H
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 361 QSEDKSTS--------DNDEARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKA 420
           Q +D  TS        DNDEARKKSVQVLLFNCM+ R PQLLL PLIKTCANNGVHFKKA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFNKVGSHSLAEANGQVDLSWQFHVRRVWQNTIQRDKGSDS------------ 480
           LFVPNTS+FNKVG+H LAEANG+VDLSWQFHVRRVW+N IQ +KG DS            
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KAEVVGKSCENSTVFSSLPMTIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
           K EV GKSCENS VFSSLPM IKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Carg02290 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 626.3 bits (1614), Expect = 2.2e-179
Identity = 323/509 (63.46%), Postives = 394/509 (77.41%), Query Frame = 0

Query: 14  AYEEALSVLSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEEPMRRMKVIHVAGTKGKGS 73
           +YEEAL+ LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + +M VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLKERTSK 133
           TCTFTESI+R+ GF TGLFTSPHLID+RERFRLDGV+ISEEKFL YFWWCYN+LKERT++
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIASLGYDHME 193
           +IPMP  FRFLALLA KIF+ E+VD AILEVGLGG FDATN VQ+P+VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 ILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELLHG 253
           ILG+TL +IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 RKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEGLTSASFDGRAQ 313
           +KLGL+GEHQY+NAGLA++L+S WL + G  ++    Q   LPEKFI+GL +AS  GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIR-HNQRGMLSHQSEDKSTSDN 373
           +V D+Y    +   LVFYLDGAHSPESME CA+WFS A++  NQ G   H     + S +
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 374 DE-ARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSVFNKVGSHS- 433
           D+ + +   Q+LLFNCM+ R P LLLP L   CA  GV+FKKALFVPN SV++KVG+ + 
Sbjct: 426 DKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHKVGTAAD 485

Query: 434 LAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPMTIKWLRDS 493
           L E + QVDLSWQF +++VW++ +Q    S+   E  G+S  NS VF+SLPM IK LRD+
Sbjct: 486 LPENDPQVDLSWQFTLQKVWESLVQ----SERDGEKDGESDGNSEVFTSLPMAIKCLRDT 545

Query: 494 VLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
           V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 VHESSSATRFQVLVTGSLHLVGDVLRLIR 570

BLAST of Carg02290 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 626.3 bits (1614), Expect = 2.2e-179
Identity = 323/509 (63.46%), Postives = 394/509 (77.41%), Query Frame = 0

Query: 14  AYEEALSVLSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEEPMRRMKVIHVAGTKGKGS 73
           +YEEAL+ LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + +M VIHVAGTKGKGS
Sbjct: 8   SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 67

Query: 74  TCTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLKERTSK 133
           TCTFTESI+R+ GF TGLFTSPHLID+RERFRLDGV+ISEEKFL YFWWCYN+LKERT++
Sbjct: 68  TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 127

Query: 134 DIPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIASLGYDHME 193
           +IPMP  FRFLALLA KIF+ E+VD AILEVGLGG FDATN VQ+P+VCGI+SLGYDHME
Sbjct: 128 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 187

Query: 194 ILGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELLHG 253
           ILG+TL +IA EKAGIFK G PAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 188 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 247

Query: 254 RKLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEGLTSASFDGRAQ 313
           +KLGL+GEHQY+NAGLA++L+S WL + G  ++    Q   LPEKFI+GL +AS  GRAQ
Sbjct: 248 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 307

Query: 314 IVADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIR-HNQRGMLSHQSEDKSTSDN 373
           +V D+Y    +   LVFYLDGAHSPESME CA+WFS A++  NQ G   H     + S +
Sbjct: 308 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 367

Query: 374 DE-ARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSVFNKVGSHS- 433
           D+ + +   Q+LLFNCM+ R P LLLP L   CA  GV+FKKALFVPN SV++KVG+ + 
Sbjct: 368 DKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHKVGTAAD 427

Query: 434 LAEANGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPMTIKWLRDS 493
           L E + QVDLSWQF +++VW++ +Q    S+   E  G+S  NS VF+SLPM IK LRD+
Sbjct: 428 LPENDPQVDLSWQFTLQKVWESLVQ----SERDGEKDGESDGNSEVFTSLPMAIKCLRDT 487

Query: 494 VLQQNRSIRLQVLVTGSLHLVGDVLKLIK 518
           V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 488 VHESSSATRFQVLVTGSLHLVGDVLRLIR 512

BLAST of Carg02290 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 565.8 bits (1457), Expect = 3.5e-161
Identity = 293/505 (58.02%), Postives = 368/505 (72.87%), Query Frame = 0

Query: 15  YEEALSVLSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMRRMKVIHVAGTKGKGST 74
           Y+  L  LSSLI  R R   +    RF L+F Y+K+L+LE+ + +MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLKERTSKD 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+G+ IS+EKF+ YFW C++KLKE+TS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 IPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIASLGYDHMEI 194
           +PMP  F FLALLA KIF+ EQVDV ILEVGLGG FDATNV+Q+P+VCGI+SLGYDHMEI
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELLHGR 254
           LG TLAEIA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEGLTSASFDGRAQI 314
           +LGL+GEHQYLNAGLA+AL ST+L   G    + ++Q+  LPEKFI GL++A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSHQSEDKSTSDNDE 374
           V D     E PE +V+YLDGAHSPESME CA WFS  I+ NQ                + 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ----------------ER 372

Query: 375 ARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSVFNKVGSHSLAEA 434
            +K+S Q+LLFNCM+ R P LLLP L   C + GV FK+A+FVPN SV+N+VGS +    
Sbjct: 373 NQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVGSSTNVGT 432

Query: 435 NGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPMTIKWLRDSVLQQ 494
             +  +SWQF ++R+W++  + +  S+SK++  GK  E S VFSSLP+ + WLRD+  +Q
Sbjct: 433 RVE-SMSWQFGLQRIWESLARGEAKSNSKSDSKGKEEEKSFVFSSLPVAVDWLRDNA-RQ 491

Query: 495 NRSIRLQVLVTGSLHLVGDVLKLIK 518
           ++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SKQVRFQVLVTGSLHLVGDLLRFIK 491

BLAST of Carg02290 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 560.1 bits (1442), Expect = 1.9e-159
Identity = 292/505 (57.82%), Postives = 367/505 (72.67%), Query Frame = 0

Query: 15  YEEALSVLSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMRRMKVIHVAGTKGKGST 74
           Y+  L  LSSLI  R R   +    RF L+F Y+K+L+LE+ + +MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLKERTSKD 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+G+ IS+EKF+ YFW C++KLKE+TS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 IPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIASLGYDHMEI 194
           +PMP  F FLALLA KIF+ EQVDV ILEVGLGG FDATN +Q+P+VCGI+SLGYDHMEI
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATN-IQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELLHGR 254
           LG TLAEIA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEGLTSASFDGRAQI 314
           +LGL+GEHQYLNAGLA+AL ST+L   G    + ++Q+  LPEKFI GL++A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSHQSEDKSTSDNDE 374
           V D     E PE +V+YLDGAHSPESME CA WFS  I+ NQ                + 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ----------------ER 372

Query: 375 ARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSVFNKVGSHSLAEA 434
            +K+S Q+LLFNCM+ R P LLLP L   C + GV FK+A+FVPN SV+N+VGS +    
Sbjct: 373 NQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVGSSTNVGT 432

Query: 435 NGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPMTIKWLRDSVLQQ 494
             +  +SWQF ++R+W++  + +  S+SK++  GK  E S VFSSLP+ + WLRD+  +Q
Sbjct: 433 RVE-SMSWQFGLQRIWESLARGEAKSNSKSDSKGKEEEKSFVFSSLPVAVDWLRDNA-RQ 490

Query: 495 NRSIRLQVLVTGSLHLVGDVLKLIK 518
           ++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SKQVRFQVLVTGSLHLVGDLLRFIK 490

BLAST of Carg02290 vs. TAIR 10
Match: AT3G55630.1 (DHFS-FPGS homolog D )

HSP 1 Score: 525.0 bits (1351), Expect = 6.8e-149
Identity = 278/505 (55.05%), Postives = 351/505 (69.50%), Query Frame = 0

Query: 15  YEEALSVLSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMRRMKVIHVAGTKGKGST 74
           Y+  L  LSSLI  R R   +    RF L+F Y+K+L+LE+ + +MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRSCGFHTGLFTSPHLIDIRERFRLDGVNISEEKFLTYFWWCYNKLKERTSKD 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+G+ IS+EKF+ YFW C++KLKE+TS  
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTS-- 132

Query: 135 IPMPHLFRFLALLALKIFSDEQVDVAILEVGLGGTFDATNVVQEPLVCGIASLGYDHMEI 194
                                +VDV ILEVGLGG FDATNV+Q+P+VCGI+SLGYDHMEI
Sbjct: 133 --------------------NEVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLAEIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVPSLDPELLHGR 254
           LG TLAEIA+EKAGIFK G PAFTV QPDEAM VL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSTWLHRAGHSKLSYMEQSRSLPEKFIEGLTSASFDGRAQI 314
           +LGL+GEHQYLNAGLA+AL ST+L   G    + ++Q+  LPEKFI GL++A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VADRYIGIESPEGLVFYLDGAHSPESMEVCARWFSAAIRHNQRGMLSHQSEDKSTSDNDE 374
           V D     E PE +V+YLDGAHSPESME CA WFS  I+ NQ                + 
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ----------------ER 372

Query: 375 ARKKSVQVLLFNCMTARVPQLLLPPLIKTCANNGVHFKKALFVPNTSVFNKVGSHSLAEA 434
            +K+S Q+LLFNCM+ R P LLLP L   C + GV FK+A+FVPN SV+N+VGS +    
Sbjct: 373 NQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVGSSTNVGT 432

Query: 435 NGQVDLSWQFHVRRVWQNTIQRDKGSDSKAEVVGKSCENSTVFSSLPMTIKWLRDSVLQQ 494
             +  +SWQF ++R+W++  + +  S+SK++  GK  E S VFSSLP+ + WLRD+  +Q
Sbjct: 433 RVE-SMSWQFGLQRIWESLARGEAKSNSKSDSKGKEEEKSFVFSSLPVAVDWLRDNA-RQ 469

Query: 495 NRSIRLQVLVTGSLHLVGDVLKLIK 518
           ++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 SKQVRFQVLVTGSLHLVGDLLRFIK 469