Carg02250 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02250
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ribonuclease DdI-like
Location	Carg_Chr15: 1463458 .. 1464589 (-)
RNA-Seq Expression	Carg02250
Synteny	Carg02250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAACAAGAACACTTTCTTCCTTCTCCTAACCATTTTGATTTTGAATGTTTTGAAAGGGTATGGTTTGGATGACATAGCTTTCATGGAATCATACGATAGTTCAAACGAGTTGGAAGAGATGAAAGCAGTTTTGTTCGATTATTATCAACTCGTACTGCAATGGCAACCAGCAACGTGCAGCAATCTCACCTGTCTGAAACCCTGGTCGTCGAGGTTCAGCATCAACGGTCTTTGGGCTGGCAGCTATTCTCGTAGCATTGGACAGTGCAGTGGCAGCGAATTTCTTCAACAAAACGTATGAATTTATTCACTCTTATGATTATTGTTAGGAATCACGACTTTCCATAATCGAGGCTCACAACTTATTTATTCGACATGTAAAGATTCTATTGACATGACTAAGTTAAGCGTATAGCTCTGATACCATGTTAAGAATCATGACTTTCCATAATGGTATGATATTCCTTACTTATAAACTAGCACTATAAAGCTTCATAGAAGGCCTATAAAGTCCTCGAACAGCCTCCCCTTAGTCAAGGCTCACAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATGACTAAGTTAAGAGCATAACTTTGATATCATATTAAGAATCACGACTCTTCACAATGATTTGATGTTATCCATTTTGAACATGAGCTCTTATTTGGTTTGGACGATAGATTTCAAGCATAAGAAGAGAGCTAGACGAGGATTGGCCAAGGCTGGTGGTCATAGGCTCAAACCCAGCAGTATGGGGCGACGAGTGGGATTTACATGGCACCTGCTTCGAGACCCCGACCTTCCAAATCTCAGACTATTTCCGGTTAGGTCTGTACCTTTTCTGGAGGAGCGACGTGCTGCAGGCGCTTGAGAAATATGGGATGGAACCCGTGGATGGGCGGCGATATCTAGCACCCGACTTTGAATCTGTCCTCAACGTAAGCTTTGGGAAGCCTGCTCTGCGCTGCAGCCGCAACGTGAAGTATAATCTTCAGTATCAGCTGGCGGAAGTGGCTCTGTGCTTCGACAAGTGTCTTCGCCACATCAACTGCCCTTCCCAGTATTCTGCTGCCAATGGCTGTCCCCGAAATCAATTTATCCTCTGGGTAAAAAACTGA

mRNA sequence

ATGGCAAACAAGAACACTTTCTTCCTTCTCCTAACCATTTTGATTTTGAATGTTTTGAAAGGGTATGGTTTGGATGACATAGCTTTCATGGAATCATACGATAGTTCAAACGAGTTGGAAGAGATGAAAGCAGTTTTGTTCGATTATTATCAACTCGTACTGCAATGGCAACCAGCAACGTGCAGCAATCTCACCTGTCTGAAACCCTGGTCGTCGAGGTTCAGCATCAACGGTCTTTGGGCTGGCAGCTATTCTCTATGGGGCGACGAGTGGGATTTACATGGCACCTGCTTCGAGACCCCGACCTTCCAAATCTCAGACTATTTCCGGTTAGGTCTGTACCTTTTCTGGAGGAGCGACGTGCTGCAGGCGCTTGAGAAATATGGGATGGAACCCGTGGATGGGCGGCGATATCTAGCACCCGACTTTGAATCTGTCCTCAACGTAAGCTTTGGGAAGCCTGCTCTGCGCTGCAGCCGCAACGTGAAGTATAATCTTCAGTATCAGCTGGCGGAAGTGGCTCTGTGCTTCGACAAGTGTCTTCGCCACATCAACTGCCCTTCCCAGTATTCTGCTGCCAATGGCTGTCCCCGAAATCAATTTATCCTCTGGGTAAAAAACTGA

Coding sequence (CDS)

ATGGCAAACAAGAACACTTTCTTCCTTCTCCTAACCATTTTGATTTTGAATGTTTTGAAAGGGTATGGTTTGGATGACATAGCTTTCATGGAATCATACGATAGTTCAAACGAGTTGGAAGAGATGAAAGCAGTTTTGTTCGATTATTATCAACTCGTACTGCAATGGCAACCAGCAACGTGCAGCAATCTCACCTGTCTGAAACCCTGGTCGTCGAGGTTCAGCATCAACGGTCTTTGGGCTGGCAGCTATTCTCTATGGGGCGACGAGTGGGATTTACATGGCACCTGCTTCGAGACCCCGACCTTCCAAATCTCAGACTATTTCCGGTTAGGTCTGTACCTTTTCTGGAGGAGCGACGTGCTGCAGGCGCTTGAGAAATATGGGATGGAACCCGTGGATGGGCGGCGATATCTAGCACCCGACTTTGAATCTGTCCTCAACGTAAGCTTTGGGAAGCCTGCTCTGCGCTGCAGCCGCAACGTGAAGTATAATCTTCAGTATCAGCTGGCGGAAGTGGCTCTGTGCTTCGACAAGTGTCTTCGCCACATCAACTGCCCTTCCCAGTATTCTGCTGCCAATGGCTGTCCCCGAAATCAATTTATCCTCTGGGTAAAAAACTGA

Protein sequence

MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWAGSYSLWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFILWVKN

Homology

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BLAST of Carg02250 vs. NCBI nr
Match: KAG7016138.1 (Ribonuclease DdI, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443.7 bits (1140), Expect = 8.5e-121
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT 60
           MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT
Sbjct: 1   MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT 60

Query: 61  CSNLTCLKPWSSRFSINGLWAGSYSLWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSD 120
           CSNLTCLKPWSSRFSINGLWAGSYSLWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSD
Sbjct: 61  CSNLTCLKPWSSRFSINGLWAGSYSLWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSD 120

Query: 121 VLQALEKYGMEPVDGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKC 180
           VLQALEKYGMEPVDGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKC
Sbjct: 121 VLQALEKYGMEPVDGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKC 180

Query: 181 LRHINCPSQYSAANGCPRNQFILWVKN 208
           LRHINCPSQYSAANGCPRNQFILWVKN
Sbjct: 181 LRHINCPSQYSAANGCPRNQFILWVKN 207

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BLAST of Carg02250 vs. NCBI nr
Match: KAG6578584.1 (Ribonuclease DdI, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 423.7 bits (1088), Expect = 9.1e-115
Identity = 206/245 (84.08%), Postives = 207/245 (84.49%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT 60
           MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT
Sbjct: 1   MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT 60

Query: 61  CSNLTCLKPWSSRFSINGLWAGSYS----------------------------------- 120
           CSNLTCLKPWSSRFSINGLWAGSYS                                   
Sbjct: 61  CSNLTCLKPWSSRFSINGLWAGSYSRSIGQCSGSEFLQQNISSIRRELDEDWPRLVVIGS 120

Query: 121 ---LWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPD 180
              +WGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPD
Sbjct: 121 NPAVWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPD 180

Query: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI 208
           FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI
Sbjct: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI 240

[Close]

BLAST of Carg02250 vs. NCBI nr
Match: XP_023551239.1 (ribonuclease DdI-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 397.5 bits (1020), Expect = 7.0e-107
Identity = 195/245 (79.59%), Postives = 199/245 (81.22%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT 60
           MAN+NTFFLLLTILILNVLKGY L+ IA +ES DS NELEEMKAV FDYYQLVLQWQPAT
Sbjct: 1   MANENTFFLLLTILILNVLKGYALNGIASVESNDSLNELEEMKAVPFDYYQLVLQWQPAT 60

Query: 61  CSNLTCLKPWSSRFSINGLWAGSYS----------------------------------- 120
           CSNLTCLKPWSSRFSINGLWAGSYS                                   
Sbjct: 61  CSNLTCLKPWSSRFSINGLWAGSYSGSIGRCNGSEFLQQNISSIRRELDEDWPSLVVIGS 120

Query: 121 ---LWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPD 180
              +WGDEWDLHGTC ETPTFQISDYFRL LYLFWRSDVLQALEKYGMEPVDGRRYLAPD
Sbjct: 121 NPAVWGDEWDLHGTCLETPTFQISDYFRLALYLFWRSDVLQALEKYGMEPVDGRRYLAPD 180

Query: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI 208
           FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI
Sbjct: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI 240

[Close]

BLAST of Carg02250 vs. NCBI nr
Match: XP_022993666.1 (ribonuclease DdI-like [Cucurbita maxima])

HSP 1 Score: 376.7 bits (966), Expect = 1.3e-100
Identity = 187/241 (77.59%), Postives = 190/241 (78.84%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT 60
           MANKNTFFLLLTILILNVLKGY       M S DSSNELEEMKAV FDYYQLVLQWQPAT
Sbjct: 1   MANKNTFFLLLTILILNVLKGYA------MASNDSSNELEEMKAVPFDYYQLVLQWQPAT 60

Query: 61  CSNLTCLKPWSSRFSINGLWAGSYS----------------------------------- 120
           CSNLTCLKPWSSRFSINGLWAGSYS                                   
Sbjct: 61  CSNLTCLKPWSSRFSINGLWAGSYSGSIGRCSGSEFLQQNISSIRREQDEDWPSLVVIGS 120

Query: 121 ---LWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPD 180
              +WG+EWDLHGTCFETPTFQISDYFRL LYLFW+SDVLQALEKYGMEP DGRRYLAPD
Sbjct: 121 NPAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPD 180

Query: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI 204
           FESVLNVSFGKPALRCSRNVKYNLQYQLAEV LCFDKCLRHINCPSQYSAANGCPRNQFI
Sbjct: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFI 235

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BLAST of Carg02250 vs. NCBI nr
Match: XP_038889664.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 273.5 bits (698), Expect = 1.5e-69
Identity = 144/264 (54.55%), Postives = 171/264 (64.77%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYGLD------------------DIAFMESYDSSN---EL 60
           M+ K + FLLL IL+LN+L G  LD                  ++AFMES ++ +   +L
Sbjct: 3   MSKKKSLFLLLCILLLNLLIGDALDELEDDDIKNEDAFNELKYELAFMESKNNDHSFYDL 62

Query: 61  EEMKAVLFDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWAGSYS-------------- 120
           EEMKAV FDYYQLVLQWQPATCSN+ CL+P SSRFSI+GLWAGSYS              
Sbjct: 63  EEMKAVPFDYYQLVLQWQPATCSNVICLRPTSSRFSIDGLWAGSYSRPIGRCSGSKFLQQ 122

Query: 121 -----------------------LWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVL 180
                                  +W + W+L GTCFETPTFQI DYFRL LYLFWRSDV 
Sbjct: 123 NITSIRKELDEDWPSLIISSNPAVWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDVQ 182

Query: 181 QALEKYGMEPVDGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLR 207
           QAL+K G+EP++G+RYL  D E+ L  SFGKPALRC+ N+KY LQ QLAEV LCFDKCLR
Sbjct: 183 QALQKSGLEPINGKRYLKSDIETALTKSFGKPALRCTLNLKYLLQSQLAEVVLCFDKCLR 242

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BLAST of Carg02250 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-11
Identity = 53/189 (28.04%), Postives = 78/189 (41.27%), Query Frame = 0


Query: 47  FDYYQLVLQWQPATC-SNLTCLKPWSSR----FSINGLWAG------------------- 106
           FD++  VLQW  A C S  +C  P + +    F I+GLW                     
Sbjct: 22  FDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDDL 81

Query: 107 ----------------------SYSLWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSD 166
                                     W  EW+ HGTC E+   Q  DYF  GL L  +++
Sbjct: 82  RVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQ-HDYFEAGLKLKQKAN 141

Query: 167 VLQALEKYGMEPVDGRRYLAPDFESVLNVSFG-KPALRCSRNVKYNLQYQLAEVALCFD- 188
           +L AL   G++P D + Y   D E+ +    G  P + C+++  +N   QL ++ LC D 
Sbjct: 142 LLHALTNAGIKP-DDKFYEMKDIENTIKQVVGFAPGIECNKDSSHN--SQLYQIYLCVDT 201

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BLAST of Carg02250 vs. ExPASy Swiss-Prot
Match: Q40381 (Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.4e-11
Identity = 48/186 (25.81%), Postives = 77/186 (41.40%), Query Frame = 0


Query: 47  FDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWAGSYS--------------------- 106
           F+Y QLVLQW  A C    C K   + F+I+GLW  + S                     
Sbjct: 24  FEYMQLVLQWPTAFCHTTPC-KRIPNNFTIHGLWPDNVSTTLNYCAAKENFKNIEDDTKK 83

Query: 107 ----------------------LWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQ 166
                                  W  E++ HG C  + ++    YF L + L  + D+L 
Sbjct: 84  DDLYKRWPDLTTAETYCKQHQNFWRHEYNKHGKCC-SESYNREQYFDLAMALKDKFDLLS 143

Query: 167 ALEKYGMEPVDGRRYLAPDFESVL-NVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLR 188
           +L  +G+ P  G +Y      S +  ++ G P L C++ +      +L E+ +CFD  ++
Sbjct: 144 SLRNHGIIPGRGMKYTVQKINSTIKKITQGYPNLSCTKGI-----MELVEIGICFDSMVK 202

[Close]

BLAST of Carg02250 vs. ExPASy Swiss-Prot
Match: Q40379 (Ribonuclease S-6 OS=Nicotiana alata OX=4087 PE=1 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 2.0e-08
Identity = 52/187 (27.81%), Postives = 77/187 (41.18%), Query Frame = 0


Query: 47  FDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWA------------------------- 106
           F+Y QLVLQW  A C    C K   S F+I+GLW                          
Sbjct: 24  FEYMQLVLQWPTAFCHTTPC-KNIPSNFTIHGLWPDNVSTTLNFCGKEDDYNIIMDGPEK 83

Query: 107 -GSYSLWGD-----------------EWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQ 166
            G Y  W D                 E+  HGTC      Q+  YFRL + L  + D+L 
Sbjct: 84  NGLYVRWPDLIREKADCMKTQNFWRREYIKHGTCCSEIYNQV-QYFRLAMALKDKFDLLT 143

Query: 167 ALEKYGMEPVDGRRYLAPDFESVL-NVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLR 189
           +L+ +G+  + G +Y      + +  V+ G P L C++        +L  V +CFD   +
Sbjct: 144 SLKNHGI--IRGYKYTVQKINNTIKTVTKGYPNLSCTKG------QELWFVGICFDSTAK 200

[Close]

BLAST of Carg02250 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.8e-07
Identity = 34/96 (35.42%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 85  SLWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPDFE 144
           + W  EW+ HGTC E+   Q  +YF+  L L  ++++L AL K G+ P DG+ Y      
Sbjct: 114 AFWEHEWEKHGTCSESVIDQ-HEYFQTALNLKQKTNLLGALTKAGINP-DGKSYSLESIR 173

Query: 145 SVLNVSFG-KPALRCSRNVKYNLQYQLAEVALCFDK 180
             +  S G  P + C+R+   N   QL +V LC D+
Sbjct: 174 DSIKESIGFTPWVECNRDGSGN--SQLYQVYLCVDR 205

[Close]

BLAST of Carg02250 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 6.1e-05
Identity = 32/103 (31.07%), Postives = 46/103 (44.66%), Query Frame = 0


Query: 87  WGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPDFESV 146
           W  EW  HGTC          YF+  L    +S++LQ L   G++P +G  Y     +  
Sbjct: 114 WSHEWLKHGTC---SALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKA 173

Query: 147 LNVSFG-KPALRCSRNVKYNLQYQLAEVALCFD-KCLRHINCP 188
           +    G  P + C  NV     +QL +V LC D    + I+CP
Sbjct: 174 IEKGVGHTPFIEC--NVDSQGNHQLYQVYLCVDSSASKFIDCP 211

[Close]

BLAST of Carg02250 vs. ExPASy TrEMBL
Match: A0A6J1JWY7 (ribonuclease DdI-like OS=Cucurbita maxima OX=3661 GN=LOC111489590 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.2e-101
Identity = 187/241 (77.59%), Postives = 190/241 (78.84%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT 60
           MANKNTFFLLLTILILNVLKGY       M S DSSNELEEMKAV FDYYQLVLQWQPAT
Sbjct: 1   MANKNTFFLLLTILILNVLKGYA------MASNDSSNELEEMKAVPFDYYQLVLQWQPAT 60

Query: 61  CSNLTCLKPWSSRFSINGLWAGSYS----------------------------------- 120
           CSNLTCLKPWSSRFSINGLWAGSYS                                   
Sbjct: 61  CSNLTCLKPWSSRFSINGLWAGSYSGSIGRCSGSEFLQQNISSIRREQDEDWPSLVVIGS 120

Query: 121 ---LWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPD 180
              +WG+EWDLHGTCFETPTFQISDYFRL LYLFW+SDVLQALEKYGMEP DGRRYLAPD
Sbjct: 121 NPAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPD 180

Query: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI 204
           FESVLNVSFGKPALRCSRNVKYNLQYQLAEV LCFDKCLRHINCPSQYSAANGCPRNQFI
Sbjct: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFI 235

[Close]

BLAST of Carg02250 vs. ExPASy TrEMBL
Match: A0A0A0KS79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G636520 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 9.7e-62
Identity = 126/237 (53.16%), Postives = 154/237 (64.98%), Query Frame = 0


Query: 10  LLTILILNVLKGYGLDDIAFMES---YDSSNELEEMKAVLFDYYQLVLQWQPATCSNLTC 69
           +L +LI + L G   ++IAF ES   + S   LE MK V FDYYQLVLQWQPATCSN  C
Sbjct: 1   MLNLLIGDALNGLE-ENIAFTESKNDHPSFYGLERMKEVPFDYYQLVLQWQPATCSNAIC 60

Query: 70  LKPWSSRFSINGLWAGSYS-------------------------------------LWGD 129
           L+PWSSRFSINGLWA SYS                                     +W +
Sbjct: 61  LRPWSSRFSINGLWAASYSRPIGRCTGNGFLQQNITSIRKELDEDWPSLVISANPAVWSE 120

Query: 130 EWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPDFESVLNV 189
            W+L GTCFE+PTFQI+DYFRL LYLFWRSDV +AL++ G+EP++G++Y   D E+ +  
Sbjct: 121 AWNLQGTCFESPTFQINDYFRLALYLFWRSDVQKALQESGIEPINGKQYEKSDIEAAITK 180

Query: 190 SFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFILWVK 207
           SFGKPALRC+ N+KY LQ QL++V LCFDKCL HI+CPS+YS A GCP    ILW K
Sbjct: 181 SFGKPALRCNLNLKYLLQSQLSQVFLCFDKCLAHIDCPSKYSPALGCPTK--ILWNK 234

[Close]

BLAST of Carg02250 vs. ExPASy TrEMBL
Match: A0A5A7TE29 (Ribonuclease DdI-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00270 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 6.9e-60
Identity = 118/222 (53.15%), Postives = 145/222 (65.32%), Query Frame = 0


Query: 25  DDIAFME---SYDSSNELEEMKAVLFDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWA 84
           +DI F+E    + S N+LE MK + FDYYQLVLQWQPATCS   CL+P+SSRFSINGLWA
Sbjct: 15  EDIDFLEPKNDHHSFNDLEGMKEIPFDYYQLVLQWQPATCSAAICLRPFSSRFSINGLWA 74

Query: 85  GSYS-------------------------------------LWGDEWDLHGTCFETPTFQ 144
           GSYS                                     +W + W+LHGTCFETPTFQ
Sbjct: 75  GSYSRLIGRCIGSRFLEQNVTSIRKELDKDWPSLIISSNPAVWSEAWNLHGTCFETPTFQ 134

Query: 145 ISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPDFESVLNVSFGKPALRCSRNVKY 204
           I+DYFRL LYLFWRSDV +AL+ +G+EP++G++Y     E+ +   FGKPALRC+ + KY
Sbjct: 135 INDYFRLALYLFWRSDVQKALQDFGIEPINGKQYEKSYIEAAITEKFGKPALRCNLSGKY 194

Query: 205 NLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFILWVK 207
            LQ QLA+V LCF+KCL  I+CPSQYS A GCP    ILW+K
Sbjct: 195 FLQSQLAQVVLCFNKCLARIDCPSQYSPALGCPTK--ILWLK 234

[Close]

BLAST of Carg02250 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.9e-17
Identity = 63/176 (35.80%), Postives = 83/176 (47.16%), Query Frame = 0


Query: 47  FDYYQLVLQWQPATCSNLT----CLKPWSSRFSINGLWAGSYS----------------- 106
           FDYYQ+VLQWQPATCS+ T    C++P   RFSI+G+W    S                 
Sbjct: 26  FDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIHGVWPSLLSGDAVCSGTPPYDGTQLN 85

Query: 107 ---------------------LWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQA 166
                                 W  EW LHGTC      Q   YF  GL+++   DVL  
Sbjct: 86  SSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCSYAYLDQF-HYFDFGLFVYGNYDVLGI 145

Query: 167 LEKYGMEPVDGRRYLAPDFESVLNVSFGK-PALRCSRNVKYNLQYQLAEVALCFDK 180
           L  Y + P +G  Y   D E+++  + GK PALRC+ N  ++   QL E++LCF K
Sbjct: 146 LNYYNVYP-NGNLYARSDVENIIYSATGKWPALRCNNNF-WSGDVQLHEISLCFRK 198

[Close]

BLAST of Carg02250 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 3.6e-16
Identity = 63/202 (31.19%), Postives = 93/202 (46.04%), Query Frame = 0


Query: 47  FDYYQLVLQWQPATCSNLT----CLKPWSSRFSINGLWAGSYS----------------- 106
           FDYYQ+VLQWQPATCS+ T    C++P   R+S++G+W    S                 
Sbjct: 25  FDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLPTCSGTPPYDARMI 84

Query: 107 ---------------------LWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQA 166
                                 W  EW  HGTC      Q   YF  GL+++   D+   
Sbjct: 85  LTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQF-HYFNFGLWVYTNYDLQSI 144

Query: 167 LEKYGMEPVDGRRYLAPDFESVLNVSFGK-PALRCSRNVKYNLQYQLAEVALCFDKCLR- 205
           L +YG+ P +G++Y     ++ +  + GK PALRC+ N +     QL E++LCF K  + 
Sbjct: 145 LYQYGIYP-NGQQYTKTVVQNAIYSATGKWPALRCNNNYRTG-NMQLHEISLCFSKNGQI 204

[Close]

BLAST of Carg02250 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-12
Identity = 53/189 (28.04%), Postives = 78/189 (41.27%), Query Frame = 0


Query: 47  FDYYQLVLQWQPATC-SNLTCLKPWSSR----FSINGLWAG------------------- 106
           FD++  VLQW  A C S  +C  P + +    F I+GLW                     
Sbjct: 22  FDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDDL 81

Query: 107 ----------------------SYSLWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSD 166
                                     W  EW+ HGTC E+   Q  DYF  GL L  +++
Sbjct: 82  RVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQ-HDYFEAGLKLKQKAN 141

Query: 167 VLQALEKYGMEPVDGRRYLAPDFESVLNVSFG-KPALRCSRNVKYNLQYQLAEVALCFD- 188
           +L AL   G++P D + Y   D E+ +    G  P + C+++  +N   QL ++ LC D 
Sbjct: 142 LLHALTNAGIKP-DDKFYEMKDIENTIKQVVGFAPGIECNKDSSHN--SQLYQIYLCVDT 201

[Close]

BLAST of Carg02250 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 61.2 bits (147), Expect = 1.1e-09
Identity = 47/188 (25.00%), Postives = 75/188 (39.89%), Query Frame = 0


Query: 47  FDYYQLVLQWQPATC-SNLTCLKPWSSR----FSINGLWAG------------------- 106
           FD++  VLQW  A C ++  C  P S +    F I+GLW                     
Sbjct: 28  FDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQFDRS 87

Query: 107 ----------------------SYSLWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSD 166
                                  ++ W  EW+ HGTC E+   Q  +YF   L L  +++
Sbjct: 88  QISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQ-HEYFENALKLKQKAN 147

Query: 167 VLQALEKYGMEPVDGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKC 188
           +LQ L+  G+ P DG   L     ++ +     P + C+++ + N   QL ++ +C D  
Sbjct: 148 LLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNA--QLHQIYICVDTS 207

[Close]

BLAST of Carg02250 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 56.6 bits (135), Expect = 2.7e-08
Identity = 34/96 (35.42%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 85  SLWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPDFE 144
           + W  EW+ HGTC E+   Q  +YF+  L L  ++++L AL K G+ P DG+ Y      
Sbjct: 114 AFWEHEWEKHGTCSESVIDQ-HEYFQTALNLKQKTNLLGALTKAGINP-DGKSYSLESIR 173

Query: 145 SVLNVSFG-KPALRCSRNVKYNLQYQLAEVALCFDK 180
             +  S G  P + C+R+   N   QL +V LC D+
Sbjct: 174 DSIKESIGFTPWVECNRDGSGN--SQLYQVYLCVDR 205

The following BLAST results are available for this feature:

BLAST of Carg02250 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016138.1	8.5e-121	100.00	Ribonuclease DdI, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6578584.1	9.1e-115	84.08	Ribonuclease DdI, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023551239.1	7.0e-107	79.59	ribonuclease DdI-like [Cucurbita pepo subsp. pepo]	[more]
XP_022993666.1	1.3e-100	77.59	ribonuclease DdI-like [Cucurbita maxima]	[more]
XP_038889664.1	1.5e-69	54.55	ribonuclease MC-like [Benincasa hispida]	[more]

BLAST of Carg02250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42815	1.5e-11	28.04	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
Q40381	3.4e-11	25.81	Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1	[more]
Q40379	2.0e-08	27.81	Ribonuclease S-6 OS=Nicotiana alata OX=4087 PE=1 SV=2	[more]
P42813	3.8e-07	35.42	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80196	6.1e-05	31.07	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]

BLAST of Carg02250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JWY7	6.2e-101	77.59	ribonuclease DdI-like OS=Cucurbita maxima OX=3661 GN=LOC111489590 PE=3 SV=1	[more]
A0A0A0KS79	9.7e-62	53.16	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G636520 PE=3 SV=1	[more]
A0A5A7TE29	6.9e-60	53.15	Ribonuclease DdI-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold89...	[more]
A0A6J1DQK2	1.9e-17	35.80	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1	[more]
A0A6J1E0X1	3.6e-16	31.19	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]

BLAST of Carg02250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26820.1	1.1e-12	28.04	ribonuclease 3	[more]
AT1G14220.1	1.1e-09	25.00	Ribonuclease T2 family protein	[more]
AT2G02990.1	2.7e-08	35.42	ribonuclease 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 47..83 e-value: 1.2E-6 score: 30.5
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 84..206 e-value: 1.5E-20 score: 75.9
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 47..203
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 84..186 e-value: 1.1E-16 score: 61.6
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 14..81
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 85..201
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 85..201
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 14..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02250-RA	Carg02250-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding