Carg02248 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02248
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ribonuclease 1-like
Location	Carg_Chr15: 1459073 .. 1460193 (-)
RNA-Seq Expression	Carg02248
Synteny	Carg02248

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCAGCGCCACACACGTGCTGCTATCATCGATCTCCTACTGTTCTTTCTTTTGTTTTCTACTTTGTATCTCATTTTAGTCGTTATCGTTCAATCCTCTACATTTGACGATTCTCTTCAAACCAACACCATCTTGACTGTTGCTGCTGCTCCTGCTACTGCCCCTGCTCATCTCCAATTCCATTCTTGGGAAAGTGACAAAAATTTTAAGAACCCTACAGCTTACCTCACAATGCATGGAATTAGATGGCATTGAATTAACCCATTAAAGTCGGTCTTTAAATTGGTTGGAGATTGGGATACACTTGCTAATCCACTTTAAGCATTCAATTCTCAATTAGTAAAAACTAAAGCTATTGATTCCTCAAATTAGAGTAGAAACCTACCATCTTTATTAGGAGTGTAATTCAATTGAAAAAAGTGAGGTAGGGTCAAGGACAAGAAATATAATCGGTTCTCGATCCTGTTTAACTAAGGGAGAAAAATCTTTTTTCACTCTCTCTCTCTCCTTCTCCATTTAGAAGGGAATTCTTCGGCGGATCTCGATAGGACTATCTGCCCATTAAATTGAACCAACGGTATATTAGGTCATAAAGAATGGCCATCTCTTGAGGAGGAGGAGAATGAGAAGATATGGAAGAAAGAATGGGAAAGGCATGGAATTTGTTCTGAACCATTGCTCACTCAGCATGCTTTCTTTGAAACTGCACTCAAGCTTAAGCAAACTTATGATCTCTTCAATATCCTAGCAAACAGAGGTGTGTGTTTCCAAAAAAAAAAAAAAAAGTAATGATACTAACTTGGAGAAGGCAATGCTGATGAATGGTTGTTTGTGATGCAGCGATATTTCCATTTGGAGAGGTGTATGGGTTAGAGAACATAAGTGATGCCATTAAAGCTGCGACGGGGCACACTCCACAAGTGGAATGCAAGTCATACAAACGCATCCCTCTGCTCTCCAACATCTTCCTTTGCTTCCAATACACTGCAGCATCTTCCATTCATATTGTCGACTGCCCCCTCGTAACAAGATGCAAATTCCAAGCCGTCCTTTTCCCTTATGATATTTTTGGTCCATCTTACAACTTCAAATCTACTACTTTAAACAATCCCCACTAG

mRNA sequence

ATGAGCCAGCGCCACACACGTGCTGCTATCATCGATCTCCTACTGTTCTTTCTTTTGTTTTCTACTTTGTATCTCATTTTAGTCGTTATCGTTCAATCCTCTACATTTGACGATTCTCTTCAAACCAACACCATCTTGACTGTTGCTGCTGCTCCTGCTACTGCCCCTGCTCATCTCCAATTCCATTCTTGGGAAAGTCATAAAGAATGGCCATCTCTTGAGGAGGAGGAGAATGAGAAGATATGGAAGAAAGAATGGGAAAGGCATGGAATTTGTTCTGAACCATTGCTCACTCAGCATGCTTTCTTTGAAACTGCACTCAAGCTTAAGCAAACTTATGATCTCTTCAATATCCTAGCAAACAGAGCGATATTTCCATTTGGAGAGGTGTATGGGTTAGAGAACATAAGTGATGCCATTAAAGCTGCGACGGGGCACACTCCACAAGTGGAATGCAAGTCATACAAACGCATCCCTCTGCTCTCCAACATCTTCCTTTGCTTCCAATACACTGCAGCATCTTCCATTCATATTGTCGACTGCCCCCTCGTAACAAGATGCAAATTCCAAGCCGTCCTTTTCCCTTATGATATTTTTGGTCCATCTTACAACTTCAAATCTACTACTTTAAACAATCCCCACTAG

Coding sequence (CDS)

ATGAGCCAGCGCCACACACGTGCTGCTATCATCGATCTCCTACTGTTCTTTCTTTTGTTTTCTACTTTGTATCTCATTTTAGTCGTTATCGTTCAATCCTCTACATTTGACGATTCTCTTCAAACCAACACCATCTTGACTGTTGCTGCTGCTCCTGCTACTGCCCCTGCTCATCTCCAATTCCATTCTTGGGAAAGTCATAAAGAATGGCCATCTCTTGAGGAGGAGGAGAATGAGAAGATATGGAAGAAAGAATGGGAAAGGCATGGAATTTGTTCTGAACCATTGCTCACTCAGCATGCTTTCTTTGAAACTGCACTCAAGCTTAAGCAAACTTATGATCTCTTCAATATCCTAGCAAACAGAGCGATATTTCCATTTGGAGAGGTGTATGGGTTAGAGAACATAAGTGATGCCATTAAAGCTGCGACGGGGCACACTCCACAAGTGGAATGCAAGTCATACAAACGCATCCCTCTGCTCTCCAACATCTTCCTTTGCTTCCAATACACTGCAGCATCTTCCATTCATATTGTCGACTGCCCCCTCGTAACAAGATGCAAATTCCAAGCCGTCCTTTTCCCTTATGATATTTTTGGTCCATCTTACAACTTCAAATCTACTACTTTAAACAATCCCCACTAG

Protein sequence

MSQRHTRAAIIDLLLFFLLFSTLYLILVVIVQSSTFDDSLQTNTILTVAAAPATAPAHLQFHSWESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAIFPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLVTRCKFQAVLFPYDIFGPSYNFKSTTLNNPH

Homology

[Close]

BLAST of Carg02248 vs. NCBI nr
Match: KAG7016136.1 (Ribonuclease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 431.4 bits (1108), Expect = 4.5e-117
Identity = 214/214 (100.00%), Postives = 214/214 (100.00%), Query Frame = 0


Query: 1   MSQRHTRAAIIDLLLFFLLFSTLYLILVVIVQSSTFDDSLQTNTILTVAAAPATAPAHLQ 60
           MSQRHTRAAIIDLLLFFLLFSTLYLILVVIVQSSTFDDSLQTNTILTVAAAPATAPAHLQ
Sbjct: 1   MSQRHTRAAIIDLLLFFLLFSTLYLILVVIVQSSTFDDSLQTNTILTVAAAPATAPAHLQ 60

Query: 61  FHSWESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILA 120
           FHSWESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILA
Sbjct: 61  FHSWESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILA 120

Query: 121 NRAIFPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVD 180
           NRAIFPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVD
Sbjct: 121 NRAIFPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVD 180

Query: 181 CPLVTRCKFQAVLFPYDIFGPSYNFKSTTLNNPH 215
           CPLVTRCKFQAVLFPYDIFGPSYNFKSTTLNNPH
Sbjct: 181 CPLVTRCKFQAVLFPYDIFGPSYNFKSTTLNNPH 214

[Close]

BLAST of Carg02248 vs. NCBI nr
Match: KAG6578582.1 (Ribonuclease 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 312.0 bits (798), Expect = 4.0e-81
Identity = 148/150 (98.67%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 124
           E  KEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI
Sbjct: 97  ELDKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 156

Query: 125 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 184
           FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV
Sbjct: 157 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 216

Query: 185 TRCKFQAVLFPYDIFGPSYNFKSTTLNNPH 215
           TRCKFQAVLFPYDIFGPSYNFKSTTLNNPH
Sbjct: 217 TRCKFQAVLFPYDIFGPSYNFKSTTLNNPH 246

[Close]

BLAST of Carg02248 vs. NCBI nr
Match: XP_022939131.1 (ribonuclease 1-like [Cucurbita moschata])

HSP 1 Score: 308.5 bits (789), Expect = 4.4e-80
Identity = 147/150 (98.00%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 124
           E  KEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI
Sbjct: 97  ELDKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 156

Query: 125 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 184
           FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV
Sbjct: 157 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 216

Query: 185 TRCKFQAVLFPYDIFGPSYNFKSTTLNNPH 215
           TRCKFQAVLFP DIFGPSYNFKSTTLNNPH
Sbjct: 217 TRCKFQAVLFPCDIFGPSYNFKSTTLNNPH 246

[Close]

BLAST of Carg02248 vs. NCBI nr
Match: XP_023550124.1 (ribonuclease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 308.5 bits (789), Expect = 4.4e-80
Identity = 146/150 (97.33%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 124
           E  KEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI
Sbjct: 97  ELDKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 156

Query: 125 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 184
           FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTA SSIHIVDCPLV
Sbjct: 157 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTATSSIHIVDCPLV 216

Query: 185 TRCKFQAVLFPYDIFGPSYNFKSTTLNNPH 215
           TRCKFQAVLFPYDIFGPSYNFKSTTL+NPH
Sbjct: 217 TRCKFQAVLFPYDIFGPSYNFKSTTLSNPH 246

[Close]

BLAST of Carg02248 vs. NCBI nr
Match: XP_022993703.1 (ribonuclease 1-like, partial [Cucurbita maxima])

HSP 1 Score: 300.1 bits (767), Expect = 1.6e-77
Identity = 142/150 (94.67%), Postives = 145/150 (96.67%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 124
           E  KEWPSLEEEENEK+WKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI
Sbjct: 129 ELDKEWPSLEEEENEKMWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 188

Query: 125 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 184
           FPFGEVY LENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTA SSIHIVDCPLV
Sbjct: 189 FPFGEVYVLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTATSSIHIVDCPLV 248

Query: 185 TRCKFQAVLFPYDIFGPSYNFKSTTLNNPH 215
           TRCKFQAVLFP+DIFGPSYN KSTTL+NPH
Sbjct: 249 TRCKFQAVLFPFDIFGPSYNLKSTTLSNPH 278

[Close]

BLAST of Carg02248 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.5e-17
Identity = 50/132 (37.88%), Postives = 72/132 (54.55%), Query Frame = 0


Query: 68  KEWPSL--EEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAIF 127
           K WP+L       E  W+ EWE+HG CSE ++ QH +F+TAL LKQ  +L   L    I 
Sbjct: 100 KSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKTNLLGALTKAGIN 159

Query: 128 PFGEVYGLENISDAIKAATGHTPQVEC-KSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 187
           P G+ Y LE+I D+IK + G TP VEC +       L  ++LC   + +    +++CP+ 
Sbjct: 160 PDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRSGSG---LIECPVF 219

Query: 188 TRCKFQA-VLFP 196
              K  A + FP
Sbjct: 220 PHGKCGAEIEFP 228

[Close]

BLAST of Carg02248 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-16
Identity = 50/133 (37.59%), Postives = 71/133 (53.38%), Query Frame = 0


Query: 68  KEWPSLEEEENE--KIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAIF 127
           +EWP+L    N+  K W  EWE+HG C+E  L QH +FE  LKLKQ  +L + L N  I 
Sbjct: 92  REWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKANLLHALTNAGIK 151

Query: 128 PFGEVYGLENISDAIKAATGHTPQVEC-KSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 187
           P  + Y +++I + IK   G  P +EC K       L  I+LC   +A+     ++CP++
Sbjct: 152 PDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTSASK---FINCPVM 211

Query: 188 T--RCKFQAVLFP 196
              RC    V FP
Sbjct: 212 PHGRCD-SRVQFP 220

[Close]

BLAST of Carg02248 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 3.6e-16
Identity = 46/120 (38.33%), Postives = 68/120 (56.67%), Query Frame = 0


Query: 68  KEWPSL--EEEENEKIWKKEWERHGICSEPLLT-QHAFFETALKLKQTYDLFNILANRAI 127
           + WP+L          W  EWE+HG C+E +LT QHA+F+ AL LK   DL +IL    I
Sbjct: 99  QNWPTLACPSGSGSTFWSHEWEKHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQGADI 158

Query: 128 FPFGEVYGLENISDAIKAATGHTPQVECK-SYKRIPLLSNIFLCFQYTAASSIHIVDCPL 184
            P GE Y L NI +AIK+A G+TP ++C         L  +++C   + +S   +++CP+
Sbjct: 159 HPDGESYDLVNIRNAIKSAIGYTPWIQCNVDQSGNSQLYQVYICVDGSGSS---LIECPI 215

[Close]

BLAST of Carg02248 vs. ExPASy Swiss-Prot
Match: P83618 (Ribonuclease-like storage protein OS=Panax ginseng OX=4054 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 41/120 (34.17%), Postives = 65/120 (54.17%), Query Frame = 0


Query: 65  ESHKEWPSLE-EEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRA 124
           E H  WPS E   E+ + W+ EW++HG CSE LL Q  +F TAL  ++ +D+  +L    
Sbjct: 92  EMHLAWPSHETNTEDIQFWEHEWKKHGRCSEALLKQTDYFRTALAFRKAFDIVGLLNQEG 151

Query: 125 IFPFGEVYGLENISDAIKAATGHTPQVE-CKSYKRIPLLSNIFLCFQYTAASSIHIVDCP 183
           I+P  ++Y  + I +AIK      P+++  K+     +L++I +C    A      VDCP
Sbjct: 152 IYPNNDLYRPKMIKEAIKKHLNAVPEIDFTKNENSEYVLTDINVCVNQQAT---RFVDCP 208

[Close]

BLAST of Carg02248 vs. ExPASy Swiss-Prot
Match: B8XY56 (Ribonuclease T2 OS=Danio rerio OX=7955 GN=rnaset2 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.1e-12
Identity = 35/91 (38.46%), Postives = 52/91 (57.14%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGIC---SEPLLTQHAFFETALKLKQTYDLFNILAN 124
           E  K WP L E  + K W  EW +HG C   SE L ++H +F  AL+L   +DL ++L  
Sbjct: 83  EMEKFWPDLLEPSSPKFWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLK 142

Query: 125 RAIFPFGEVYGLENISDAIKAATGHTPQVEC 153
             I P  + Y LE++ +AI +A G  P+++C
Sbjct: 143 NQIVPSEKHYTLEDVEEAITSAYGVKPKIQC 173

[Close]

BLAST of Carg02248 vs. ExPASy TrEMBL
Match: A0A6J1FF23 (ribonuclease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111445130 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 2.1e-80
Identity = 147/150 (98.00%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 124
           E  KEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI
Sbjct: 97  ELDKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 156

Query: 125 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 184
           FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV
Sbjct: 157 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 216

Query: 185 TRCKFQAVLFPYDIFGPSYNFKSTTLNNPH 215
           TRCKFQAVLFP DIFGPSYNFKSTTLNNPH
Sbjct: 217 TRCKFQAVLFPCDIFGPSYNFKSTTLNNPH 246

[Close]

BLAST of Carg02248 vs. ExPASy TrEMBL
Match: A0A6J1K331 (ribonuclease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111489614 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 7.6e-78
Identity = 142/150 (94.67%), Postives = 145/150 (96.67%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 124
           E  KEWPSLEEEENEK+WKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI
Sbjct: 129 ELDKEWPSLEEEENEKMWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 188

Query: 125 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 184
           FPFGEVY LENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTA SSIHIVDCPLV
Sbjct: 189 FPFGEVYVLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTATSSIHIVDCPLV 248

Query: 185 TRCKFQAVLFPYDIFGPSYNFKSTTLNNPH 215
           TRCKFQAVLFP+DIFGPSYN KSTTL+NPH
Sbjct: 249 TRCKFQAVLFPFDIFGPSYNLKSTTLSNPH 278

[Close]

BLAST of Carg02248 vs. ExPASy TrEMBL
Match: A0A6J1C1D3 (ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111006533 PE=3 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 8.7e-66
Identity = 121/138 (87.68%), Postives = 131/138 (94.93%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 124
           E  +EWPSLEEEENEK WKKEWERHGICSEPLLT+HAFFETALKLKQTYDLFN+LAN+AI
Sbjct: 111 ELDREWPSLEEEENEKEWKKEWERHGICSEPLLTEHAFFETALKLKQTYDLFNMLANKAI 170

Query: 125 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 184
           FPFGEVYGLENISDAIKAATGHTPQVEC+SYK IPLLSNIFLCFQYT A+SI I+DCPL+
Sbjct: 171 FPFGEVYGLENISDAIKAATGHTPQVECESYKHIPLLSNIFLCFQYT-ATSIDIIDCPLI 230

Query: 185 TRCKFQAVLFPYDIFGPS 203
           TRC FQA+LFPYD+FGPS
Sbjct: 231 TRCNFQAILFPYDLFGPS 247

[Close]

BLAST of Carg02248 vs. ExPASy TrEMBL
Match: A0A0A0KSB0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G636550 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 7.9e-59
Identity = 110/138 (79.71%), Postives = 123/138 (89.13%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 124
           E  +EWPSLE EENE+IW+KEWE HGICS+PLLTQHAFFETALKLKQT+D+F ILANR I
Sbjct: 113 ELDREWPSLEVEENEEIWRKEWENHGICSQPLLTQHAFFETALKLKQTFDIFTILANRGI 172

Query: 125 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 184
           FPFGEVY LENISDAI+ ATGHTPQVECKSYK+IPLLSNIFLCF+Y   ++IHIVDCPL+
Sbjct: 173 FPFGEVYDLENISDAIRDATGHTPQVECKSYKQIPLLSNIFLCFKYN-DNAIHIVDCPLI 232

Query: 185 TRCKFQAVLFPYDIFGPS 203
            RC FQA+LFPY  FGPS
Sbjct: 233 RRCNFQAILFPYAQFGPS 249

[Close]

BLAST of Carg02248 vs. ExPASy TrEMBL
Match: A0A1S3C9W2 (ribonuclease 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498487 PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.1e-57
Identity = 108/138 (78.26%), Postives = 121/138 (87.68%), Query Frame = 0


Query: 65  ESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAI 124
           E  +EWPSLE EENE++WKKEWE HGICS+PLLTQH+FFETALKLKQ +DLF ILANR I
Sbjct: 103 ELDREWPSLEVEENEEMWKKEWESHGICSQPLLTQHSFFETALKLKQNFDLFTILANRGI 162

Query: 125 FPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 184
           FPFGEVY LENIS+AI+ ATGHTPQVEC+SYK IPLLSNIFLCFQY   ++IHIVDCPL+
Sbjct: 163 FPFGEVYDLENISNAIRDATGHTPQVECQSYKNIPLLSNIFLCFQYN-RNAIHIVDCPLI 222

Query: 185 TRCKFQAVLFPYDIFGPS 203
            RC FQA+LFPY  FGPS
Sbjct: 223 RRCNFQAILFPYSQFGPS 239

[Close]

BLAST of Carg02248 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-20
Identity = 51/122 (41.80%), Postives = 69/122 (56.56%), Query Frame = 0


Query: 68  KEWPSLEEEENE--KIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAIF 127
           K WP+L    NE    W+ EWE+HG CSE ++ QH +FE ALKLKQ  +L  IL N  I 
Sbjct: 98  KNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKLKQKANLLQILKNSGIN 157

Query: 128 PFGEVYGLENISDAIKAATGHTPQVEC-KSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 187
           P    Y L+ I++AIK   G TP +EC K  +R   L  I++C      S    ++CP++
Sbjct: 158 PDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNAQLHQIYICVD---TSGTEFIECPVL 216

[Close]

BLAST of Carg02248 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 90.5 bits (223), Expect = 1.8e-18
Identity = 50/132 (37.88%), Postives = 72/132 (54.55%), Query Frame = 0


Query: 68  KEWPSL--EEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAIF 127
           K WP+L       E  W+ EWE+HG CSE ++ QH +F+TAL LKQ  +L   L    I 
Sbjct: 100 KSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKTNLLGALTKAGIN 159

Query: 128 PFGEVYGLENISDAIKAATGHTPQVEC-KSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 187
           P G+ Y LE+I D+IK + G TP VEC +       L  ++LC   + +    +++CP+ 
Sbjct: 160 PDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRSGSG---LIECPVF 219

Query: 188 TRCKFQA-VLFP 196
              K  A + FP
Sbjct: 220 PHGKCGAEIEFP 228

[Close]

BLAST of Carg02248 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-17
Identity = 50/133 (37.59%), Postives = 71/133 (53.38%), Query Frame = 0


Query: 68  KEWPSLEEEENE--KIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRAIF 127
           +EWP+L    N+  K W  EWE+HG C+E  L QH +FE  LKLKQ  +L + L N  I 
Sbjct: 92  REWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKANLLHALTNAGIK 151

Query: 128 PFGEVYGLENISDAIKAATGHTPQVEC-KSYKRIPLLSNIFLCFQYTAASSIHIVDCPLV 187
           P  + Y +++I + IK   G  P +EC K       L  I+LC   +A+     ++CP++
Sbjct: 152 PDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTSASK---FINCPVM 211

Query: 188 T--RCKFQAVLFP 196
              RC    V FP
Sbjct: 212 PHGRCD-SRVQFP 220

[Close]

BLAST of Carg02248 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 78.6 bits (192), Expect = 6.9e-15
Identity = 43/122 (35.25%), Postives = 58/122 (47.54%), Query Frame = 0


Query: 64  WESHKEWPSLEEEENEKIWKKEWERHGICSEPLLTQHAFFETALKLKQTYDLFNILANRA 123
           W     W     E N  +W+ EW +HG C + +  QH++F T LK K    L NIL  + 
Sbjct: 98  WTEWGVWACPSNETN--LWEHEWNKHGTCVQSIFDQHSYFRTNLKFKHKVHLLNILIQKG 157

Query: 124 IFPFGEVYGLENISDAIKAATGHTPQVEC-KSYKRIPLLSNIFLCFQYTAASSIHIVDCP 183
           I P    Y L+ I +AIK A G  P +EC +  K    L  I++C    A      V+CP
Sbjct: 158 IKPNDGFYSLDEIKNAIKCAIGFAPGIECNEDVKGNKQLFQIYICLDNYAK---EFVECP 214

Query: 184 LV 185
            V
Sbjct: 218 YV 214

[Close]

BLAST of Carg02248 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 47.0 bits (110), Expect = 2.2e-05
Identity = 34/110 (30.91%), Postives = 49/110 (44.55%), Query Frame = 0


Query: 68  KEWPSLE-------EEENEKIWKKEWERHGICSEPLL-TQHAFFETALKLKQTYDLFNIL 127
           K WPSL               W  EWE+HG CS P+   ++ +F T L L   +++ ++L
Sbjct: 105 KYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVL 164

Query: 128 ANRA-IFPFGEVYGLENISDAIKAATGHTPQVECKSYKRIPLLSNIFLCF 169
                +    E Y L  I  AI+ A   TP+V CK       +  I +CF
Sbjct: 165 YQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKR----DAIDEIRICF 210

The following BLAST results are available for this feature:

BLAST of Carg02248 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016136.1	4.5e-117	100.00	Ribonuclease 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6578582.1	4.0e-81	98.67	Ribonuclease 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022939131.1	4.4e-80	98.00	ribonuclease 1-like [Cucurbita moschata]	[more]
XP_023550124.1	4.4e-80	97.33	ribonuclease 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022993703.1	1.6e-77	94.67	ribonuclease 1-like, partial [Cucurbita maxima]	[more]

BLAST of Carg02248 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42813	2.5e-17	37.88	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	2.1e-16	37.59	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P80022	3.6e-16	38.33	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P83618	1.1e-12	34.17	Ribonuclease-like storage protein OS=Panax ginseng OX=4054 PE=1 SV=2	[more]
B8XY56	9.1e-12	38.46	Ribonuclease T2 OS=Danio rerio OX=7955 GN=rnaset2 PE=2 SV=1	[more]

BLAST of Carg02248 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FF23	2.1e-80	98.00	ribonuclease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111445130 PE=3 SV=1	[more]
A0A6J1K331	7.6e-78	94.67	ribonuclease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111489614 PE=3 SV=1	[more]
A0A6J1C1D3	8.7e-66	87.68	ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111006533 PE=3 SV=1	[more]
A0A0A0KSB0	7.9e-59	79.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G636550 PE=3 SV=1	[more]
A0A1S3C9W2	1.1e-57	78.26	ribonuclease 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498487 PE=3 SV=1	[more]

BLAST of Carg02248 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	1.1e-20	41.80	Ribonuclease T2 family protein	[more]
AT2G02990.1	1.8e-18	37.88	ribonuclease 1	[more]
AT1G26820.1	1.5e-17	37.59	ribonuclease 3	[more]
AT1G14210.1	6.9e-15	35.25	Ribonuclease T2 family protein	[more]
AT2G39780.1	2.2e-05	30.91	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 67..170 e-value: 2.7E-24 score: 86.3
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 63..202
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 39..195 e-value: 2.4E-29 score: 104.9
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 68..189
None	No IPR available	PANTHER	PTHR11240:SF61	EXTRACELLULAR RIBONUCLEASE LE-LIKE ISOFORM X1	coord: 63..202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02248-RA	Carg02248-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0140657	ATP-dependent activity
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding