Carg02247 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02247
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Defective in cullin neddylation protein
Location	Carg_Chr15: 1454072 .. 1458966 (+)
RNA-Seq Expression	Carg02247
Synteny	Carg02247

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGCCAAGACAGAGAACCAAAACCAGAGCTGAAGGCGAAGGAGGGAAGAAAGAGTAACGAGAGAGCTTCCTTCTCTGTTATTGAGTTGGGGTCTGGAGGATTCTTCTTCTGCTCCTTGCTCCCCAACAATCTAACTTCCATGACTTCCGTTGTGGATTTCTTGCATATGAATCGCTTCGATATCGTCCAGATTTATCGTCAATACTGTGGTAACACCTTTCTTCTCCTCCTCACTGTTTCGAATTCTCGATTCAGTTCATTTCTCTTGAATCTTCTCCGATGCCTTTTCTATCCACAATTTGTTTCGTTATGCCTTTAGAATCTTAATATTTCTGCCCTTGGGTAGATATCTGATTCAATATTTGAGGATCTTTAATAGAAATCTATGTATGCGTCTGCTTGCTCATGTATGTTAGCTTAGAAGATATACGTATGTCAAAGATTTGGATGTTTTTATTTATCTTTTAGAAGATGGTTATTTGCTAACTGATTTTGGATGCTTAAGATGTTATGACGTATTTAGTCCGAAGAAGTCTAGGTGCCTTTATTTTTTGGAACCTGCATTAATAGTTTGGATGATAAAGAATGTAAAGGATTCAGTTTATGGCGATTCTCTTGCCGAGTATGATGCATGAGTGTTAATTTCAATTTTCTGTTCTGGTTAGTCCGATTACTTGGAGAACAAATGAGCAATGTTATTACTCGTAGGTTGTCGGAAGAATAATCCAAGAATTACTCATTATTTTGTCACCTTGTGAACATTTGATAGAACTGGAACAATGAATAGAGGATATGTGTGGCCCTTGCACAAGGATGGCGCAAACTTCGAGAAGGGATCCAAAAAAGGAATTAGTAATATTGTATTATGATTTCAGAGTCGGAATTGCAGCCTTTTAGGAGGAGAAGTTTGAGTCTTACTTAATCATTCCAGATTGATAATAGTAATATTGTTGTTATACTAGTTTCGCTTTTCTTTAGATGACAGAAAACCAGAATAAAAGGAGCCGAGAAAATGTGGGGTTGGTGGAGCTGAGTGGAAACACTAAACTCTATTGAAAGAAAGTAAAATAGTATCATGTTTTGGCTCTTGATCGTTTAGAATCAAGCTACTTATATATGCTTATCACTTTTTCGTATGAATGTAATATGAATAAATGGTTATCATGTGCATGGGTTTCTTCTGACTTCCGAGCTCGTCAATGGCTTCCTATTTTTGTTTGAATGTATTACCTCTTAGTTGTCATGGAAAAAAGATCATGGTTCTGATATTTGAAACCCCCCTCATTATTGGTTGGACTATGAATTAATTGAACTCTAGTCAGATGTGCATTACGTTAGGAACTCAAATTAGCAATATAAGCGAGTCTTAACTTGTTTACAAACTTGTTTATAGATATTCGATCTCAAAATGGCCTTTATCATGGGAATGAAGACTCTGGACATGTTGAATCACAAATAGCTAAAAGTTCAAAGCAGGCTCTTGCAGAACTCTTCCTTTATGTGAAATCATCGCTGCAGATGGGGTATATCCCTCAAAACCTGTACTATAGCCCTCGAGTTGTTTTTTCAGATGCACTTTGTGTCAAATGGACCATGGTATTCTCGGATACGCAGAGTTACAACTCAATATTTTTTGTCCTTTCAAAAGAAGATATTTTCCAAACATAATTTAACCGACAGATGTATGAAACTAAGATAATTTATCAACTCAATTATCAAACCAAGGTAATTTTTAATAGACATGGATGTTTGCTAGTTTGATTTCATTTAGTTCTGGGTCTAGTATTTTCTGGAAGTTCAATAAAAAGAAATGTTACAACTTGATAACGGAGGCCATCCTAAGTCATTTTTGTACTTCTGAAGCCGATCACAAGAAATAGCTGTTGATTACAGTATAAAAGTTTTTCTTATACTAACCTAGGTTATCTGAAAAGCATTTATTACACTGGCAGGAACTCAATTGTTGATGAACTAGCGAAGGTGATGTCACATCCAAACTTGATGGTAAACTGTTCCTTCCTAACAGTTTTCCTGGTTTCATACTATTCTCTGGTTTCCTTCAATTTGGCAAGCTTTTGGTATTAGCTGGTGGCAATATTTTTACTTTGTGCTTTGAATATGACAATTTTATTTTGTAGGTAGACTTCTCTGAATTTTCACGCTTTTACGAGTTTGTTTTTTTTGTGAGCCGTGAACATGGTCAAAAGAATATCAGTAAGATTCCTTCTTTTGACTTGGATATAAAAAATATGTTTTGGTTTCCCAACATAGATAACATGAAATGTGCACTAGGTAAGTCAAAGTTTCTCTTACATTCCGACAAGTTATTTAGTTTTTTTATTTTATTTGAATTACAGCTGTTAGTAGGGCAGTTAGAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGGTATCCTTTTCCACCTTCTCTTTCTTTCCTTTTTTTTTAGAACTCAAGGACACAGTGATACAACCTACAGAAATATCCTGTTCAAATAAGGTATTTTCCTTTTGTTGAGGTACCCTTTTCCCCTCCTCTTTCGTTTGGTTATTTAGGCAGAATGATAATAATCAGGAAACCTGAATTTCTACCTGAAACTATTTTCATCGACTTGGGTTTGTGGGACCCAACAATTATGAAACTTACGTATATGCTAAATACCCATGAATGAACTTTCCTATGTAGTTGCCCTCTGCAGACATGTTTGGATACACGTTCATATGTGTTTTACAAAAATGTATGCATTTGTCTCAATTTTTGAAGTAGATGAGATTGGGAAAATTTGGGTATTTTTATACCTAAGCTTTTAATCTATCTGATTTCTTCTATCTCGGTTTATGAATTTATGTTATTTTTGTGGTTTTATTCTTCAATTCGTTCCATGAAATAAAATTGCGATGTAGTGCAACTTATCTCCAGGGTTCTGATCTATTTAAGTTCCATAGAACAATCAGCGACACAATATTTCTGAGGATACTTGGCTGCAAGTCTTGGCTTTCAGCTGTTGTGTACATGAAAATCTGGAAGGGTATGACCCGGAAGGTATTTGTACTTTTTTTTAAAAAAAAATAATTCTTAATGGAAGTATTTTCGTAGATATTATTTGAATTAAAAATGTTAAGAATTGGGAGTATGTCAAGCAATCCGCCTCCTTATAACTGTTGAAGTTTTTCAGTATAATTAAATTCTATCACAAGAAAAAGTATGTTGTCTATTTATATAAGAAAATTTTAATGCTTCACTTTCATGTTTAACCTTCTGTTTTGGTTCTTCTGTGGATGCTTGAAATATCATGAGAATGTGAATATTAATTGATCTCATTATGTTGTTATGGTGGTTTGGCATTATTATTTTGTTTCCTCAAGTATAGGCTTAACGCCTAAAAAGGGTTTTGAGGCTTATGCATTTGGGAGGCAAGCCTCAAGGTAGGGTACGATTGTAAGCTTTCAAGGCAAAGCCTTACTTTGATTCGTAAGAAACTCATGCTTCTTGGAGTTCTACGAGAATACACTGAGATTAGAAGTTCATTAGTTATATGATGGCTAGAATAATACAGCCAACGGCTGTCTGCATACTCTTAAAAAATGGAAGTCCACTTTTGAAAGCAATATGTGTATACACTTAATACCTTCAGTACTTTTATCACTAAAATAAAGAACAAATTTTTTTTACAATAGTTTGATTCAGTTAGCTATAGTGATGATGTTAAAGATGAGCTTCATAGTCATGACTTAAAGAATTAAAAATGTATGGATACTCGATTTTTCTTTTCAGTACTTTTATATTATCATCGTGTTTAATAGTTGCACAGTTCTATATTTTCTGAATTCATTAAATTTTTTCTAATTAACTTGAGGATTATGCCTCACTTCTCTTGAAGAAAGAGCCTTAAGACTACCCCTTAGTCTTTTGGAATGTTGGGTATGCTTTTAAATAAGCACAATATTTCTCTTTTTTTACTGTAATGTCTGGAGTTACTCAGTTGCGTTCTTCAATCAGGTGCTTGGCCTGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGTTCGTATGTGGAGATGCGATGAGTTTGAAGTTGCATGTGGTTAAATAATTCTGGCCTTTATATCATTTTAAGACTGTCGTTTTTTTGTCACTGAAGCCTTGTACTAACACTTGATATGCTGATAGCATGTAGTTATTGTGCAGGCAGTCAGGATCTAATAAAATTTCAAAGTTATATTGTACTTGTGGAGACTCAGAATCACAGTCTGGTGTCTTTGAAGATTCTCTTCCTGGTATAGACTACGTTCAACTGTCAAAAAAAAAAAAAAAAAAAAGTACTGTTTTACATATTGGACTTCCATGGCATGCAGAATGATATATAAGCCGTGTTTTACTGGTTTGGATTATGTCAGGATTAAAGTCTTTCCCTGGTTTGAAGAGGAAATTACCCGAGGACATGCAAGTGGACGACATTACGTCTCTGAGTGACCCTAGAACCGGCTCCACGGAGTTAATTCCTGTTACGAGCCTCAAGAAAACTAGATTCATGGGTTGCAGGCCAGTGAACTTGGAGGTAAACTTACCGTCATGCACTGCAGATGAGAACATAGATATGGTTCGAAATAACGGTGCAGTAGGCTGCAAATCTCCTTGTGCTATTGAAGGTTGTCTGTCAAAAGGTTTTGCTGGGCTTTTCTCGACATGTTCAATCCTAGGATTGGATCAAGAAAGGAAAGCGTCATTCACGTGATCATGTCCGATTTAGCTCTAATGATTTTTGAAGGTAGTATGTTCTATAATGTGGTTATATCTTTATTTTTTGTTATGGTAACAGCATTTTTAAACGGATGAGTTTGTGATTTGGCAGCAAACTAAA

mRNA sequence

AAAAGCCAAGACAGAGAACCAAAACCAGAGCTGAAGGCGAAGGAGGGAAGAAAGAGTAACGAGAGAGCTTCCTTCTCTGTTATTGAGTTGGGGTCTGGAGGATTCTTCTTCTGCTCCTTGCTCCCCAACAATCTAACTTCCATGACTTCCGTTGTGGATTTCTTGCATATGAATCGCTTCGATATCGTCCAGATTTATCGTCAATACTGTGATATTCGATCTCAAAATGGCCTTTATCATGGGAATGAAGACTCTGGACATGTTGAATCACAAATAGCTAAAAGTTCAAAGCAGGCTCTTGCAGAACTCTTCCTTTATGTGAAATCATCGCTGCAGATGGGGAACTCAATTGTTGATGAACTAGCGAAGGTGATGTCACATCCAAACTTGATGGTAGACTTCTCTGAATTTTCACGCTTTTACGAGTTTGTTTTTTTTGTGAGCCGTGAACATGGTCAAAAGAATATCACTGTTAGTAGGGCAGTTAGAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGAACAATCAGCGACACAATATTTCTGAGGATACTTGGCTGCAAGTCTTGGCTTTCAGCTGTTGTGTACATGAAAATCTGGAAGGGTATGACCCGGAAGGTGCTTGGCCTGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGCAGTCAGGATCTAATAAAATTTCAAAGTTATATTGTACTTGTGGAGACTCAGAATCACAGTCTGGTGTCTTTGAAGATTCTCTTCCTGGATTAAAGTCTTTCCCTGGTTTGAAGAGGAAATTACCCGAGGACATGCAAGTGGACGACATTACGTCTCTGAGTGACCCTAGAACCGGCTCCACGGAGTTAATTCCTGTTACGAGCCTCAAGAAAACTAGATTCATGGGTTGCAGGCCAGTGAACTTGGAGGTAAACTTACCGTCATGCACTGCAGATGAGAACATAGATATGGTTCGAAATAACGGTGCAGTAGGCTGCAAATCTCCTTGTGCTATTGAAGGTTGTCTGTCAAAAGGTTTTGCTGGGCTTTTCTCGACATGTTCAATCCTAGGATTGGATCAAGAAAGGAAAGCGTCATTCACGTGATCATGTCCGATTTAGCTCTAATGATTTTTGAAGGTTATATCTTTATTTTTTGTTATGGTAACAGCATTTTTAAACGGATGAGTTTGTGATTTGGCAGCAAACTAAA

Coding sequence (CDS)

AAAAGCCAAGACAGAGAACCAAAACCAGAGCTGAAGGCGAAGGAGGGAAGAAAGAGTAACGAGAGAGCTTCCTTCTCTGTTATTGAGTTGGGGTCTGGAGGATTCTTCTTCTGCTCCTTGCTCCCCAACAATCTAACTTCCATGACTTCCGTTGTGGATTTCTTGCATATGAATCGCTTCGATATCGTCCAGATTTATCGTCAATACTGTGATATTCGATCTCAAAATGGCCTTTATCATGGGAATGAAGACTCTGGACATGTTGAATCACAAATAGCTAAAAGTTCAAAGCAGGCTCTTGCAGAACTCTTCCTTTATGTGAAATCATCGCTGCAGATGGGGAACTCAATTGTTGATGAACTAGCGAAGGTGATGTCACATCCAAACTTGATGGTAGACTTCTCTGAATTTTCACGCTTTTACGAGTTTGTTTTTTTTGTGAGCCGTGAACATGGTCAAAAGAATATCACTGTTAGTAGGGCAGTTAGAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGAACAATCAGCGACACAATATTTCTGAGGATACTTGGCTGCAAGTCTTGGCTTTCAGCTGTTGTGTACATGAAAATCTGGAAGGGTATGACCCGGAAGGTGCTTGGCCTGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGCAGTCAGGATCTAATAAAATTTCAAAGTTATATTGTACTTGTGGAGACTCAGAATCACAGTCTGGTGTCTTTGAAGATTCTCTTCCTGGATTAAAGTCTTTCCCTGGTTTGAAGAGGAAATTACCCGAGGACATGCAAGTGGACGACATTACGTCTCTGAGTGACCCTAGAACCGGCTCCACGGAGTTAATTCCTGTTACGAGCCTCAAGAAAACTAGATTCATGGGTTGCAGGCCAGTGAACTTGGAGGTAAACTTACCGTCATGCACTGCAGATGAGAACATAGATATGGTTCGAAATAACGGTGCAGTAGGCTGCAAATCTCCTTGTGCTATTGAAGGTTGTCTGTCAAAAGGTTTTGCTGGGCTTTTCTCGACATGTTCAATCCTAGGATTGGATCAAGAAAGGAAAGCGTCATTCACGTGA

Protein sequence

KSQDREPKPELKAKEGRKSNERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSDPRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGCLSKGFAGLFSTCSILGLDQERKASFT

Homology

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BLAST of Carg02247 vs. NCBI nr
Match: KAG7016135.1 (DCN1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 761.5 bits (1965), Expect = 3.3e-216
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0


Query: 1   KSQDREPKPELKAKEGRKSNERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRF 60
           KSQDREPKPELKAKEGRKSNERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRF
Sbjct: 1   KSQDREPKPELKAKEGRKSNERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRF 60

Query: 61  DIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDE 120
           DIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDE
Sbjct: 61  DIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDE 120

Query: 121 LAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLLNQWC 180
           LAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLLNQWC
Sbjct: 121 LAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLLNQWC 180

Query: 181 DFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKIS 240
           DFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKIS
Sbjct: 181 DFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKIS 240

Query: 241 KLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSDPRTGSTELIPVTS 300
           KLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSDPRTGSTELIPVTS
Sbjct: 241 KLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSDPRTGSTELIPVTS 300

Query: 301 LKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGCLSKGFAGLFSTCS 360
           LKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGCLSKGFAGLFSTCS
Sbjct: 301 LKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGCLSKGFAGLFSTCS 360

Query: 361 ILGLDQERKASFT 374
           ILGLDQERKASFT
Sbjct: 361 ILGLDQERKASFT 373

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BLAST of Carg02247 vs. NCBI nr
Match: XP_022993364.1 (uncharacterized protein LOC111489407 [Cucurbita maxima])

HSP 1 Score: 716.5 bits (1848), Expect = 1.2e-202
Identity = 353/373 (94.64%), Postives = 357/373 (95.71%), Query Frame = 0


Query: 1   KSQDREPKPELKAKEGRKSNERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRF 60
           K   R  K   KAKEGRKS ERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRF
Sbjct: 5   KKPRRRTKTRAKAKEGRKSIERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRF 64

Query: 61  DIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDE 120
           DIVQIYRQYCDIRSQNGLYHGNED GHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDE
Sbjct: 65  DIVQIYRQYCDIRSQNGLYHGNEDCGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDE 124

Query: 121 LAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLLNQWC 180
           LAKVMSHPNLMVDFSEFSRFYEFVFFVSRE+GQKNITVSRAVRAWRLVLDGRFRLLNQWC
Sbjct: 125 LAKVMSHPNLMVDFSEFSRFYEFVFFVSRENGQKNITVSRAVRAWRLVLDGRFRLLNQWC 184

Query: 181 DFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKIS 240
           DFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYR+SGSNKI 
Sbjct: 185 DFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKIP 244

Query: 241 KLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSDPRTGSTELIPVTS 300
           KLYCTCGDSESQSGVFEDSLPGLKSFPGLKRK PEDMQVDDI S SDPRTGS ELIPVTS
Sbjct: 245 KLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKFPEDMQVDDIMSPSDPRTGSMELIPVTS 304

Query: 301 LKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGCLSKGFAGLFSTCS 360
           +KK+RFMGCRPVNLEVNLPSCTADENIDMVR NGAVGCKSPCAIEGCLSKGFAGLFSTCS
Sbjct: 305 VKKSRFMGCRPVNLEVNLPSCTADENIDMVRYNGAVGCKSPCAIEGCLSKGFAGLFSTCS 364

Query: 361 ILGLDQERKASFT 374
           ILGLDQERKASFT
Sbjct: 365 ILGLDQERKASFT 377

[Close]

BLAST of Carg02247 vs. NCBI nr
Match: XP_023550121.1 (DCN1-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023550122.1 DCN1-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650.2 bits (1676), Expect = 1.1e-182
Identity = 314/326 (96.32%), Postives = 320/326 (98.16%), Query Frame = 0


Query: 48  MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 107
           MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV
Sbjct: 1   MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 60

Query: 108 KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRL 167
           KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSRE+GQKNITVSRAVRAWRL
Sbjct: 61  KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSRENGQKNITVSRAVRAWRL 120

Query: 168 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 227
           VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 180

Query: 228 EHMYRQSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSD 287
           EHMYR+SGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVD I SLSD
Sbjct: 181 EHMYRRSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDGIMSLSD 240

Query: 288 PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGC 347
           PRTGS ELIPVTS+KK+RFMGCRPVNLEVNLPSCTAD N DMVR NGAVGCK+PCAIEGC
Sbjct: 241 PRTGSMELIPVTSVKKSRFMGCRPVNLEVNLPSCTADANTDMVRPNGAVGCKAPCAIEGC 300

Query: 348 LSKGFAGLFSTCSILGLDQERKASFT 374
           LSKGFAGLFSTCS+LGLDQERKASFT
Sbjct: 301 LSKGFAGLFSTCSVLGLDQERKASFT 326

[Close]

BLAST of Carg02247 vs. NCBI nr
Match: XP_022938999.1 (DCN1-like protein 1 [Cucurbita moschata] >XP_022939000.1 DCN1-like protein 1 [Cucurbita moschata])

HSP 1 Score: 648.7 bits (1672), Expect = 3.1e-182
Identity = 316/325 (97.23%), Postives = 320/325 (98.46%), Query Frame = 0


Query: 48  MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 107
           MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV
Sbjct: 1   MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 60

Query: 108 KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRL 167
           KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSRE+GQKNITV RAVRAWRL
Sbjct: 61  KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSRENGQKNITVGRAVRAWRL 120

Query: 168 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 227
           VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 180

Query: 228 EHMYRQSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSD 287
           EHMYR+SGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDI SLSD
Sbjct: 181 EHMYRRSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDIMSLSD 240

Query: 288 PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGC 347
           PRTGS ELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVR+NGAVGCKSPCAIEG 
Sbjct: 241 PRTGSMELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRHNGAVGCKSPCAIEGF 300

Query: 348 LSKGFAGLFSTCSILGLDQERKASF 373
           LSKGFAGLFST SILGLD+ERKASF
Sbjct: 301 LSKGFAGLFSTRSILGLDRERKASF 325

[Close]

BLAST of Carg02247 vs. NCBI nr
Match: KAG6578581.1 (DCN1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 617.5 bits (1591), Expect = 7.7e-173
Identity = 298/301 (99.00%), Postives = 299/301 (99.34%), Query Frame = 0


Query: 48  MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 107
           MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV
Sbjct: 1   MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 60

Query: 108 KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRL 167
           KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRL
Sbjct: 61  KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRL 120

Query: 168 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 227
           VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 180

Query: 228 EHMYRQSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSD 287
           EHMYRQSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSD
Sbjct: 181 EHMYRQSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSD 240

Query: 288 PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGC 347
           PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIE  
Sbjct: 241 PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEAL 300

Query: 348 L 349
           +
Sbjct: 301 M 301

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BLAST of Carg02247 vs. ExPASy Swiss-Prot
Match: Q9MBG8 (Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=AAR3 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.2e-75
Identity = 156/311 (50.16%), Postives = 198/311 (63.67%), Query Frame = 0


Query: 59  RFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIV 118
           RFDI +IYR++C IRS   L +     G  ESQ    SK+A+ +L   V++  Q  NSI 
Sbjct: 9   RFDIFEIYRRFCAIRSGQQLCNKKPCDGE-ESQRFNLSKEAITQLLYLVENKFQARNSIF 68

Query: 119 DELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLLNQ 178
           DEL K+MS  +LMVDF+EF+ FY+FVFF+ RE+GQKNIT+SRA+ AW+LVL GRFRLLN+
Sbjct: 69  DELFKLMSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNR 128

Query: 179 WCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNK 238
           WCDF+E NQRHNISEDTW QVLAFS CVHENLEGYD EGAWPVLIDDFVEHMY   G NK
Sbjct: 129 WCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSILGPNK 188

Query: 239 ISKLYCTCGDSESQSGVFEDS---------LPGLKSFPGLKRKLPE--DMQVDDITSLSD 298
            + L+C CGD+ES+S ++++            GL++ PGLKRK  +  D + +D      
Sbjct: 189 DTSLFCKCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKRKTSKKNDEEEEDEDEEVL 248

Query: 299 PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGC 358
               S+ L+    +K +            N P C++               KSPC+IE  
Sbjct: 249 ETQNSSSLLNFKRIKTS------------NSPRCSS---------------KSPCSIERS 291

[Close]

BLAST of Carg02247 vs. ExPASy Swiss-Prot
Match: Q9VUQ8 (DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 2.2e-21
Identity = 48/124 (38.71%), Postives = 71/124 (57.26%), Query Frame = 0


Query: 106 YVKSSLQMGNSIVDEL-AKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRA 165
           ++     +G   +D+L  K+      + D  +F  FY F F  +++ GQK I +  A+  
Sbjct: 118 FINGMCDLGIDSIDKLKTKLPILEQELNDAGKFKDFYHFTFNYAKDPGQKGIDLEMAIAY 177

Query: 166 WRLVLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLID 225
           W +VL GRF+ L+ WC F+E   +  IS DTW  +L F+  + + +  YD EGAWPVLID
Sbjct: 178 WCIVLSGRFKFLDIWCQFLEEKHKRAISRDTWNLLLDFATNIDDRMSNYDSEGAWPVLID 237

Query: 226 DFVE 229
           DFVE
Sbjct: 238 DFVE 241

[Close]

BLAST of Carg02247 vs. ExPASy Swiss-Prot
Match: Q6PH85 (DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 7.1e-20
Identity = 42/97 (43.30%), Postives = 62/97 (63.92%), Query Frame = 0


Query: 133 DFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLLNQWCDFVENNQRHNIS 192
           D ++F  FY+F F  ++  GQK + +  AV  W+LVL GRF+ L+ W  F+  + + +I 
Sbjct: 150 DTAKFKDFYQFTFTFAKNPGQKGLDLEMAVAYWKLVLSGRFKFLDLWNTFLMEHHKRSIP 209

Query: 193 EDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEH 230
            DTW  +L F   + +++  YD EGAWPVLIDDFVE+
Sbjct: 210 RDTWNLLLDFGNMIADDMSNYDEEGAWPVLIDDFVEY 246

[Close]

BLAST of Carg02247 vs. ExPASy Swiss-Prot
Match: Q5ZKU1 (DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 9.3e-20
Identity = 56/196 (28.57%), Postives = 99/196 (50.51%), Query Frame = 0


Query: 57  MNRFDIVQIYRQYCDIRSQN-----GLYHGNEDSGHVESQIA--------KSSKQALAEL 116
           ++R  + Q+Y +Y D + +N     G+    +D     + I         +++ Q     
Sbjct: 60  LDRKKLEQLYNRYKDPQDENKIGIDGIQQFCDDLALDPASITVLIIAWKFRAATQCEFSK 119

Query: 117 FLYVKSSLQMGNSIVDEL-AKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAV 176
             ++    ++G   +++L A++      + +   F  FY+F F  ++  GQK + +  A+
Sbjct: 120 LEFMDGMTELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAI 179

Query: 177 RAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVL 236
             W LVL+GRF+ L+ W  F+  + + +I +DTW  +L FS  + +++  YD EGAWPVL
Sbjct: 180 AYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSTMIADDMSNYDEEGAWPVL 239

Query: 237 IDDFVEHMYRQSGSNK 239
           IDDFVE    Q    K
Sbjct: 240 IDDFVEFARPQIAGTK 255

[Close]

BLAST of Carg02247 vs. ExPASy Swiss-Prot
Match: Q9QZ73 (DCN1-like protein 1 OS=Mus musculus OX=10090 GN=Dcun1d1 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 9.3e-20
Identity = 46/134 (34.33%), Postives = 76/134 (56.72%), Query Frame = 0


Query: 106 YVKSSLQMGNSIVDEL-AKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRA 165
           ++    ++G   +++L A++      + +   F  FY+F F  ++  GQK + +  A+  
Sbjct: 122 FMDGMTELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAY 181

Query: 166 WRLVLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLID 225
           W LVL+GRF+ L+ W  F+  + + +I +DTW  +L FS  + +++  YD EGAWPVLID
Sbjct: 182 WNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSSMIADDMSNYDEEGAWPVLID 241

Query: 226 DFVEHMYRQSGSNK 239
           DFVE    Q    K
Sbjct: 242 DFVEFARPQIAGTK 255

[Close]

BLAST of Carg02247 vs. ExPASy TrEMBL
Match: A0A6J1JZZ8 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111489407 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 5.9e-203
Identity = 353/373 (94.64%), Postives = 357/373 (95.71%), Query Frame = 0


Query: 1   KSQDREPKPELKAKEGRKSNERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRF 60
           K   R  K   KAKEGRKS ERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRF
Sbjct: 5   KKPRRRTKTRAKAKEGRKSIERASFSVIELGSGGFFFCSLLPNNLTSMTSVVDFLHMNRF 64

Query: 61  DIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDE 120
           DIVQIYRQYCDIRSQNGLYHGNED GHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDE
Sbjct: 65  DIVQIYRQYCDIRSQNGLYHGNEDCGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDE 124

Query: 121 LAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLLNQWC 180
           LAKVMSHPNLMVDFSEFSRFYEFVFFVSRE+GQKNITVSRAVRAWRLVLDGRFRLLNQWC
Sbjct: 125 LAKVMSHPNLMVDFSEFSRFYEFVFFVSRENGQKNITVSRAVRAWRLVLDGRFRLLNQWC 184

Query: 181 DFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKIS 240
           DFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYR+SGSNKI 
Sbjct: 185 DFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKIP 244

Query: 241 KLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSDPRTGSTELIPVTS 300
           KLYCTCGDSESQSGVFEDSLPGLKSFPGLKRK PEDMQVDDI S SDPRTGS ELIPVTS
Sbjct: 245 KLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKFPEDMQVDDIMSPSDPRTGSMELIPVTS 304

Query: 301 LKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGCLSKGFAGLFSTCS 360
           +KK+RFMGCRPVNLEVNLPSCTADENIDMVR NGAVGCKSPCAIEGCLSKGFAGLFSTCS
Sbjct: 305 VKKSRFMGCRPVNLEVNLPSCTADENIDMVRYNGAVGCKSPCAIEGCLSKGFAGLFSTCS 364

Query: 361 ILGLDQERKASFT 374
           ILGLDQERKASFT
Sbjct: 365 ILGLDQERKASFT 377

[Close]

BLAST of Carg02247 vs. ExPASy TrEMBL
Match: A0A6J1FFS5 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111445042 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.5e-182
Identity = 316/325 (97.23%), Postives = 320/325 (98.46%), Query Frame = 0


Query: 48  MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 107
           MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV
Sbjct: 1   MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 60

Query: 108 KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRL 167
           KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSRE+GQKNITV RAVRAWRL
Sbjct: 61  KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSRENGQKNITVGRAVRAWRL 120

Query: 168 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 227
           VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 180

Query: 228 EHMYRQSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSD 287
           EHMYR+SGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDI SLSD
Sbjct: 181 EHMYRRSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDIMSLSD 240

Query: 288 PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGC 347
           PRTGS ELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVR+NGAVGCKSPCAIEG 
Sbjct: 241 PRTGSMELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRHNGAVGCKSPCAIEGF 300

Query: 348 LSKGFAGLFSTCSILGLDQERKASF 373
           LSKGFAGLFST SILGLD+ERKASF
Sbjct: 301 LSKGFAGLFSTRSILGLDRERKASF 325

[Close]

BLAST of Carg02247 vs. ExPASy TrEMBL
Match: A0A1S3CB35 (Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 2.1e-155
Identity = 270/326 (82.82%), Postives = 296/326 (90.80%), Query Frame = 0


Query: 48  MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 107
           MTS++ F HMNRFDIVQIYRQYCDIRSQNG YHGNEDSG+ E+Q++KSSKQALAEL +YV
Sbjct: 1   MTSLLGFSHMNRFDIVQIYRQYCDIRSQNG-YHGNEDSGYAETQMSKSSKQALAELLIYV 60

Query: 108 KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRL 167
           +SSLQMGNS+V EL+K+MS+ N MVDFSEFSRFYEF FFV RE+GQKNITVS AVRAWRL
Sbjct: 61  QSSLQMGNSVVYELSKLMSYLNFMVDFSEFSRFYEFXFFVCRENGQKNITVSMAVRAWRL 120

Query: 168 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 227
           VLDGRFRLLNQWCDFVENNQRHNISEDTW QVLAFS CVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180

Query: 228 EHMYRQSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSD 287
           EHMYR+SGSNK+SKL CTCGDSES+SGVFEDSL GLK FPGLKRKLPEDMQ+D I S SD
Sbjct: 181 EHMYRRSGSNKVSKLCCTCGDSESESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSD 240

Query: 288 PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGC 347
           PRTG+ E  PVTS+KK+RFM CRPVNLE+NLPSCTA ENI++VR++  VG KSPCA+EGC
Sbjct: 241 PRTGAMEFTPVTSVKKSRFMACRPVNLEINLPSCTAAENIEVVRHSSTVGSKSPCAVEGC 300

Query: 348 LSKGFAGLFSTCSILGLDQERKASFT 374
           LSKGFAGLFST   LGLDQERKASFT
Sbjct: 301 LSKGFAGLFSTRLFLGLDQERKASFT 325

[Close]

BLAST of Carg02247 vs. ExPASy TrEMBL
Match: A0A0A0KU82 (Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G636560 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 5.6e-153
Identity = 269/326 (82.52%), Postives = 292/326 (89.57%), Query Frame = 0


Query: 48  MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 107
           MTS++ F HMNRFDIVQIYRQYCDIRSQNG YHGNEDSG+ E+Q+ KSSKQALAEL +YV
Sbjct: 1   MTSLLGFSHMNRFDIVQIYRQYCDIRSQNG-YHGNEDSGNAETQMCKSSKQALAELLIYV 60

Query: 108 KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRL 167
           +SSLQMGNS+V EL+K+MS+ N MVDFSEFSRFYEFVFFV RE+GQKNITVS AVRAWRL
Sbjct: 61  QSSLQMGNSVVYELSKLMSYLNFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRL 120

Query: 168 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 227
           VLDGRFRLLNQWCDFVENNQRHNISEDTW QVLAFS CVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180

Query: 228 EHMYRQSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSD 287
           EHMYR+SGSNK+SKL C CGDSE +SGVFEDSL GLK FPGLKRKLPEDMQ+D I S SD
Sbjct: 181 EHMYRRSGSNKVSKLSCNCGDSE-ESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSD 240

Query: 288 PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGC 347
           PRT + E  PVTS+KK+RFM CRPVNLE+N PSCTA ENI+MVR+N  VG KSPCA+EGC
Sbjct: 241 PRTSAMEFTPVTSVKKSRFMACRPVNLEINSPSCTAAENIEMVRHNSTVGSKSPCAVEGC 300

Query: 348 LSKGFAGLFSTCSILGLDQERKASFT 374
           LSKGFAGLFST   LGLDQERKASFT
Sbjct: 301 LSKGFAGLFSTRLFLGLDQERKASFT 324

[Close]

BLAST of Carg02247 vs. ExPASy TrEMBL
Match: A0A6J1GSP1 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111457165 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.8e-144
Identity = 255/324 (78.70%), Postives = 281/324 (86.73%), Query Frame = 0


Query: 57  MNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNS 116
           MNRFDIVQIY QYC IRSQNGL HGN   GHVE+Q+AKSSKQALAEL +YV+SSL  GNS
Sbjct: 1   MNRFDIVQIYHQYCGIRSQNGLCHGNGGFGHVETQMAKSSKQALAELLIYVQSSLHTGNS 60

Query: 117 IVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLL 176
           I+ EL+K+MS+ NLMVDFSEFSRFYEFVFFV RE+GQKNITV RA++AWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 177 NQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGS 236
           NQWCDFVENNQRHNISEDTWLQVLAFS CVHENLEGYDPEGAWPVLIDDFVEHMYR SGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 237 NKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDITSLSDPRTGSTELI 296
           NKISKL C+CG SESQSG  ED  PGLK  PGLKRKLPED+Q+D + S SDP TGS EL 
Sbjct: 181 NKISKLRCSCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMELS 240

Query: 297 PVTSLKKTRFMGCRPVNLEVNLPSC------TADENIDMVRNNGAVGC-KSPCAIEGCLS 356
           PVTS+KK+RFM C+PVNLE+N PSC       AD N ++VRNNGA+GC K+PCA+EGCLS
Sbjct: 241 PVTSIKKSRFMACKPVNLEINGPSCNAAAAAAADGNTEIVRNNGAMGCSKAPCAVEGCLS 300

Query: 357 KGFAGLFSTCSILGLDQERKASFT 374
           +GFAGLFST S LGLDQERK+SFT
Sbjct: 301 QGFAGLFSTRSFLGLDQERKSSFT 324

[Close]

BLAST of Carg02247 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 285.0 bits (728), Expect = 8.5e-77
Identity = 156/311 (50.16%), Postives = 198/311 (63.67%), Query Frame = 0


Query: 59  RFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIV 118
           RFDI +IYR++C IRS   L +     G  ESQ    SK+A+ +L   V++  Q  NSI 
Sbjct: 9   RFDIFEIYRRFCAIRSGQQLCNKKPCDGE-ESQRFNLSKEAITQLLYLVENKFQARNSIF 68

Query: 119 DELAKVMSHPNLMVDFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGRFRLLNQ 178
           DEL K+MS  +LMVDF+EF+ FY+FVFF+ RE+GQKNIT+SRA+ AW+LVL GRFRLLN+
Sbjct: 69  DELFKLMSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNR 128

Query: 179 WCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNK 238
           WCDF+E NQRHNISEDTW QVLAFS CVHENLEGYD EGAWPVLIDDFVEHMY   G NK
Sbjct: 129 WCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSILGPNK 188

Query: 239 ISKLYCTCGDSESQSGVFEDS---------LPGLKSFPGLKRKLPE--DMQVDDITSLSD 298
            + L+C CGD+ES+S ++++            GL++ PGLKRK  +  D + +D      
Sbjct: 189 DTSLFCKCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKRKTSKKNDEEEEDEDEEVL 248

Query: 299 PRTGSTELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRNNGAVGCKSPCAIEGC 358
               S+ L+    +K +            N P C++               KSPC+IE  
Sbjct: 249 ETQNSSSLLNFKRIKTS------------NSPRCSS---------------KSPCSIERS 291

[Close]

BLAST of Carg02247 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 97.1 bits (240), Expect = 3.3e-20
Identity = 43/100 (43.00%), Postives = 62/100 (62.00%), Query Frame = 0


Query: 133 DFSEFSRFYEFVFFVSREHGQKNITVSRAVRAWRLVLDGR-FRLLNQWCDFVENNQRHNI 192
           D  +F   Y F F  ++E GQK++ +  A+  W+L+   R + L+  WCDF+++     I
Sbjct: 144 DEQKFHEIYNFAFGWAKEKGQKSLALDTAIGMWQLLFAEREWPLVTHWCDFLQDRHNKAI 203

Query: 193 SEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMY 232
           S+DTW Q+L FS  V   L  YD EGAWP LID+FVE++Y
Sbjct: 204 SKDTWAQLLEFSRMVDPVLSNYDAEGAWPYLIDEFVEYLY 243

[Close]

BLAST of Carg02247 vs. TAIR 10
Match: AT1G15860.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 48.9 bits (115), Expect = 1.0e-05
Identity = 31/113 (27.43%), Postives = 58/113 (51.33%), Query Frame = 0


Query: 120 ELAKVMSHPNLMVDFSEFSRFYEFVF-FVSREHGQKNITVSRAVRAWRLVLDGRFRL-LN 179
           EL K +  P      S F+ FY + F +   E  QK+I +    +   +V+   FR  ++
Sbjct: 114 ELEKEVRRP------SNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVD 173

Query: 180 QWCDFVE-NNQRHNISEDTWLQVLAFSCCV-HENLEGYDPEGAWPVLIDDFVE 229
            + ++++  N    I+ D W+ +  F   +   ++  Y+PE AWP+++D+FVE
Sbjct: 174 YFVEYLKIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVE 220

[Close]

BLAST of Carg02247 vs. TAIR 10
Match: AT1G15860.3 (Domain of unknown function (DUF298) )

HSP 1 Score: 46.6 bits (109), Expect = 5.1e-05
Identity = 33/122 (27.05%), Postives = 54/122 (44.26%), Query Frame = 0


Query: 120 ELAKVMSHPNLMVDFSEFSRFYEFVF-FVSREHGQKNITVSRAVRAWRLVLDGRFR---- 179
           EL K +  P      S F+ FY + F +   E  QK+I +    +   +V+   FR    
Sbjct: 114 ELEKEVRRP------SNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVD 173

Query: 180 ----LLNQWC---DFVENNQRHNISEDTWLQVLAFSCCV-HENLEGYDPEGAWPVLIDDF 229
                L  W      +  N    I+ D W+ +  F   +   ++  Y+PE AWP+++D+F
Sbjct: 174 YFVEYLKVWITQKSHIIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNF 229

The following BLAST results are available for this feature:

BLAST of Carg02247 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016135.1	3.3e-216	100.00	DCN1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022993364.1	1.2e-202	94.64	uncharacterized protein LOC111489407 [Cucurbita maxima]	[more]
XP_023550121.1	1.1e-182	96.32	DCN1-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023550122.1 DCN1-like prote...	[more]
XP_022938999.1	3.1e-182	97.23	DCN1-like protein 1 [Cucurbita moschata] >XP_022939000.1 DCN1-like protein 1 [Cu...	[more]
KAG6578581.1	7.7e-173	99.00	DCN1-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg02247 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG8	1.2e-75	50.16	Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9VUQ8	2.2e-21	38.71	DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2	[more]
Q6PH85	7.1e-20	43.30	DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1	[more]
Q5ZKU1	9.3e-20	28.57	DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1	[more]
Q9QZ73	9.3e-20	34.33	DCN1-like protein 1 OS=Mus musculus OX=10090 GN=Dcun1d1 PE=2 SV=1	[more]

BLAST of Carg02247 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JZZ8	5.9e-203	94.64	Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]
A0A6J1FFS5	1.5e-182	97.23	Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A1S3CB35	2.1e-155	82.82	Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 ...	[more]
A0A0A0KU82	5.6e-153	82.52	Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G6365...	[more]
A0A6J1GSP1	2.8e-144	78.70	Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of Carg02247 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28970.1	8.5e-77	50.16	Domain of unknown function (DUF298)	[more]
AT3G12760.1	3.3e-20	43.00	CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP...	[more]
AT1G15860.1	1.0e-05	27.43	Domain of unknown function (DUF298)	[more]
AT1G15860.3	5.1e-05	27.05	Domain of unknown function (DUF298)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005176	Potentiating neddylation domain	PFAM	PF03556	Cullin_binding	coord: 132..229 e-value: 4.4E-28 score: 98.1
IPR005176	Potentiating neddylation domain	PROSITE	PS51229	DCUN1	coord: 133..231 score: 19.657709
IPR042460	DCN1-like, PONY binding domain	GENE3D	1.10.238.200	Cullin, PONY binding domain	coord: 133..241 e-value: 6.2E-34 score: 118.4
IPR014764	Defective-in-cullin neddylation protein	PANTHER	PTHR12281	RP42 RELATED	coord: 59..273
None	No IPR available	PANTHER	PTHR12281:SF31	DEFECTIVE IN CULLIN NEDDYLATION PROTEIN	coord: 59..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02247-RA	Carg02247-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051443	positive regulation of ubiquitin-protein transferase activity
biological_process	GO:0045116	protein neddylation
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0097602	cullin family protein binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0032182	ubiquitin-like protein binding