Carg02235 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02235
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pectinesterase
Location	Carg_Chr15: 1395372 .. 1397979 (-)
RNA-Seq Expression	Carg02235
Synteny	Carg02235

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGTTTAACCGAAGAGTCCGTCTAGTTGGCCGCCTTGCCGCCTCAATCGAAAGAAATATAGATAAATCAGCTGTACCCATGAAATTTAATGGCTCCGATGCAAAATTGGAGGAGTTTGCTTGTCTTTCCACTCTATAAATACAATTGATTTGGTCTTCATTTTCATTCCGCTTCCCAACATCCAGAAGGCGCGAGCTTCGAGACTGCGTTTAATGGCGGCCCCGCCCAGGGTGCTCACGGTGGCGCAAGACGGCTGTGGGGATTTCCGTACGGTTCAGGAAGCCATAGACGCGGTTCCATTCAGCAATACTTGCCGGACTGTGATCCGTGTGTCTCCTGGGATTTACAGGCAGCCTCTCTATGTGCCTAAGACCAAGAATTTCATCACCTTCGCGGGTTTGAACCCTGAGACTACCGTTCTCACTTGGGATAATACTGCCACCAAAATTAGCCACCACCAAGTCGGTTTCTGATACGATTTTATCATTTGATATCTGGGTTTGCGTTTAATTTGAAGAATGAAGTTCATGTGTTCCTCTATCTCTGTCTCTGGCCAGGCATCTCGTGTCATTGGAACTGGGACTTTTGGTTGCGGGAGTACCATTGTGGAAGGGGAGGATTTCATTGCTGAGAATATCACATTTGAGAACTCTTCCCCTCAGGTACCCTTTTACTTTTACGACCTCTTTGCTCATCTTATCTTGGATTGACCTAAGAGTTAAAACCCCTTTTTAGTTCATAGTTTGAGGTTGATTTTGAAATTGTAGAATGAGGATCTTTGTTGGATCTGTATTTTTCTGATGAACTTCCTTAGCGTTTTGAAGTTGGTATTGTTTGTTTAGATATGTTGTGGGTTGTTTGTTTTGGACAGGGTTCTGGGCAAGCCGTGGCTATCAGAGTTACAGCAGATCGTTGTGCCTTTTACAATTGCAGGTTTCTCGGCTGGCAGGTATTCACAATCCTCTAATTCAGTTCGTTAGTGGGCAGTAAGTTCTAGAGGTCAAACAATATTTCTGGGAAATCCCTACCGTTTTTTTTTTCCTTCACCCCTCCTTAAATCTGAATGGAAAAGGCCTTTTCCTATAAGAACTTGTGGGTTGTTTGGGTGGATATTCAAGACACCATAATTATGGAGGTGCTTCGGTAAGAGTTATCTCCCAAGCTACTAAAAAGAGTGTCATCTTTAGATCCCACATCGATGGAGAGGGAAACCAAACATTCTTTATGAAACCTTTCTAACATATGTGTTTTAAAAACCTTGAGAAAACCGGAAAGAGAGCAAAATGTGCTAGCAGTGGGTTTGGGCTGTTACACCAGGCAGTATCGAGGAGGATGCGCCCGAGGGGTGGACACGAGGCAGTGTGCCAACAAGGACACTAGGCCTTGGAGGGGTGAACTGTGAGATCGCATATTGGTTGGAACCGTTATAGAGGTGTCTGGATTGAATTGCGATTGGAATCGTTATAGAGGTGTCTGGATTGAATTTCCTCTCTTAGCAGCTATATATAGTGTCCAACACATAGGGAAGTTGGCTCAATTGCCATCAAGCAAATCTGTAATACTGTTAGGATCTACAGTTTGATGTTGTTGTCGACATCCAATGGCTCTATCCTTTGGCTAGTTCAATCAGCCAACGATCACAGTGAATGGTTTTTCTGCACTTCTATAGCCTGCTGCTATTGTTTCTTCTTCTATCATATTTTTGGATAATCTGATGCAGGATACTCTGTACCTACATTATGGAAAACAGTACTTGAAGGATTGCTATATCGAAGGAAGTGTGGACTTCATATTTGGCAACAGCACTGCTCTGTTGGAGCGTTGCCATATCCACTGCAAGTCGCATGGCTTTATTACTGCACAGAGCAGGAAATCCTCTCAAGAGACCACAGGTTATGTATTCTTGAGGTGTGTGATCACGGGCAGTGCCGGGACTTCGTACGTTCATCTCGGACGACCATGGGGACCGTTTGCAAGGGTGGTCTTTGCTTACACATATATGGATGCATGTATCAAACCAGCAGGCTGGGACAACTGGGGAAAAACTGAAAACGAGAGAACTGCTTGTTTTTATGAGTACAAGTACGTCGTCGTGTTTCATCGACATTGATTATATGATGAATGGGAACATTACCAAATGTGTAGATTACCTGATAGGGGAATTGTGTTTTTCGGTACAGGTGTTTCGGGCCAGGGAGTTGCTCAATGAGCAGAGTGTGTTGGGCAAGAGAATTAGTAGATGAAGAAGCTGATGAGTTCATTCTGCATCAATTTATCGATCCCGACGTCGAGAGGCCTTGGCTATGCCGGAGGATGGCATTGAGGATACCCTTTTCTGCTTAAGAATGGGTGAGTGTTACAAAGGTTTGGAAGTAATCCACCAGTAGAGAAGTAGTGTCTAAATGTTGAACATTTGTTGCTTGCTGGTCCGTTAGAAGAAGGAACATTCATATCCTATATGGGTGGCATAGGGCTTCTTGTAGCCTCAGATTGTGAATTTTGGCCATGTATTGACCACTTTTTCATATTACTCGCCTATCTTTATATATATTTTTTCAGCATTATATTTTAATCTGTGTTAAATATTTATTGGATGCCCAATTTTCT

mRNA sequence

TTTGTTTAACCGAAGAGTCCGTCTAGTTGGCCGCCTTGCCGCCTCAATCGAAAGAAATATAGATAAATCAGCTGTACCCATGAAATTTAATGGCTCCGATGCAAAATTGGAGGAGTTTGCTTGTCTTTCCACTCTATAAATACAATTGATTTGGTCTTCATTTTCATTCCGCTTCCCAACATCCAGAAGGCGCGAGCTTCGAGACTGCGTTTAATGGCGGCCCCGCCCAGGGTGCTCACGGTGGCGCAAGACGGCTGTGGGGATTTCCGTACGGTTCAGGAAGCCATAGACGCGGTTCCATTCAGCAATACTTGCCGGACTGTGATCCGTGTGTCTCCTGGGATTTACAGGCAGCCTCTCTATGTGCCTAAGACCAAGAATTTCATCACCTTCGCGGGTTTGAACCCTGAGACTACCGTTCTCACTTGGGATAATACTGCCACCAAAATTAGCCACCACCAAGCATCTCGTGTCATTGGAACTGGGACTTTTGGTTGCGGGAGTACCATTGTGGAAGGGGAGGATTTCATTGCTGAGAATATCACATTTGAGAACTCTTCCCCTCAGGGTTCTGGGCAAGCCGTGGCTATCAGAGTTACAGCAGATCGTTGTGCCTTTTACAATTGCAGGTTTCTCGGCTGGCAGCCTGCTGCTATTGTTTCTTCTTCTATCATATTTTTGGATAATCTGATGCAGGATACTCTGTACCTACATTATGGAAAACAGTACTTGAAGGATTGCTATATCGAAGGAAGTGTGGACTTCATATTTGGCAACAGCACTGCTCTGTTGGAGCGTTGCCATATCCACTGCAAGTCGCATGGCTTTATTACTGCACAGAGCAGGAAATCCTCTCAAGAGACCACAGGTTATGTATTCTTGAGGTGTGTGATCACGGGCAGTGCCGGGACTTCGTACGTTCATCTCGGACGACCATGGGGACCGTTTGCAAGGGTGGTCTTTGCTTACACATATATGGATGCATGTATCAAACCAGCAGGCTGGGACAACTGGGGAAAAACTGAAAACGAGAGAACTGCTTGTTTTTATGAGTACAAGTGTTTCGGGCCAGGGAGTTGCTCAATGAGCAGAGTGTGTTGGGCAAGAGAATTAGTAGATGAAGAAGCTGATGAGTTCATTCTGCATCAATTTATCGATCCCGACGTCGAGAGGCCTTGGCTATGCCGGAGGATGGCATTGAGGATACCCTTTTCTGCTTAAGAATGGGTGAGTGTTACAAAGGTTTGGAAGTAATCCACCAGTAGAGAAGTAGTGTCTAAATGTTGAACATTTGTTGCTTGCTGGTCCGTTAGAAGAAGGAACATTCATATCCTATATGGGTGGCATAGGGCTTCTTGTAGCCTCAGATTGTGAATTTTGGCCATGTATTGACCACTTTTTCATATTACTCGCCTATCTTTATATATATTTTTTCAGCATTATATTTTAATCTGTGTTAAATATTTATTGGATGCCCAATTTTCT

Coding sequence (CDS)

ATGGCGGCCCCGCCCAGGGTGCTCACGGTGGCGCAAGACGGCTGTGGGGATTTCCGTACGGTTCAGGAAGCCATAGACGCGGTTCCATTCAGCAATACTTGCCGGACTGTGATCCGTGTGTCTCCTGGGATTTACAGGCAGCCTCTCTATGTGCCTAAGACCAAGAATTTCATCACCTTCGCGGGTTTGAACCCTGAGACTACCGTTCTCACTTGGGATAATACTGCCACCAAAATTAGCCACCACCAAGCATCTCGTGTCATTGGAACTGGGACTTTTGGTTGCGGGAGTACCATTGTGGAAGGGGAGGATTTCATTGCTGAGAATATCACATTTGAGAACTCTTCCCCTCAGGGTTCTGGGCAAGCCGTGGCTATCAGAGTTACAGCAGATCGTTGTGCCTTTTACAATTGCAGGTTTCTCGGCTGGCAGCCTGCTGCTATTGTTTCTTCTTCTATCATATTTTTGGATAATCTGATGCAGGATACTCTGTACCTACATTATGGAAAACAGTACTTGAAGGATTGCTATATCGAAGGAAGTGTGGACTTCATATTTGGCAACAGCACTGCTCTGTTGGAGCGTTGCCATATCCACTGCAAGTCGCATGGCTTTATTACTGCACAGAGCAGGAAATCCTCTCAAGAGACCACAGGTTATGTATTCTTGAGGTGTGTGATCACGGGCAGTGCCGGGACTTCGTACGTTCATCTCGGACGACCATGGGGACCGTTTGCAAGGGTGGTCTTTGCTTACACATATATGGATGCATGTATCAAACCAGCAGGCTGGGACAACTGGGGAAAAACTGAAAACGAGAGAACTGCTTGTTTTTATGAGTACAAGTGTTTCGGGCCAGGGAGTTGCTCAATGAGCAGAGTGTGTTGGGCAAGAGAATTAGTAGATGAAGAAGCTGATGAGTTCATTCTGCATCAATTTATCGATCCCGACGTCGAGAGGCCTTGGCTATGCCGGAGGATGGCATTGAGGATACCCTTTTCTGCTTAA

Protein sequence

MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA

Homology

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BLAST of Carg02235 vs. NCBI nr
Match: KAG7016123.1 (Pectinesterase 31 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 713.8 bits (1841), Expect = 7.1e-202
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR
Sbjct: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL
Sbjct: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA
Sbjct: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 335

[Close]

BLAST of Carg02235 vs. NCBI nr
Match: KAG6578565.1 (Pectinesterase 31, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 672.2 bits (1733), Expect = 2.4e-189
Identity = 318/335 (94.93%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNCRFLGW                 QDTLYLHYGKQYLKDCYIEG
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHYGKQYLKDCYIEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR
Sbjct: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL
Sbjct: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA
Sbjct: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 318

[Close]

BLAST of Carg02235 vs. NCBI nr
Match: XP_022938497.1 (pectinesterase 31 [Cucurbita moschata])

HSP 1 Score: 671.8 bits (1732), Expect = 3.1e-189
Identity = 317/335 (94.63%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNCRFLGW                 QDTLYLHYGKQYLKDCYIEG
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHYGKQYLKDCYIEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLERCH+HCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR
Sbjct: 181 SVDFIFGNSTALLERCHVHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL
Sbjct: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA
Sbjct: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 318

[Close]

BLAST of Carg02235 vs. NCBI nr
Match: XP_022993645.1 (pectinesterase 31 [Cucurbita maxima])

HSP 1 Score: 668.7 bits (1724), Expect = 2.6e-188
Identity = 316/335 (94.33%), Postives = 317/335 (94.63%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNCRFLGW                 QDTLYLHYGKQYLKDCYIEG
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHYGKQYLKDCYIEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAG+SYVHLGR
Sbjct: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGSSYVHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSC MSRVCWAREL
Sbjct: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCPMSRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA
Sbjct: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 318

[Close]

BLAST of Carg02235 vs. NCBI nr
Match: XP_023551220.1 (pectinesterase 31 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023551221.1 pectinesterase 31 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 666.4 bits (1718), Expect = 1.3e-187
Identity = 315/335 (94.03%), Postives = 316/335 (94.33%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRVLTVAQDGC DFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAASPRVLTVAQDGCADFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNCRFLGW                 QDTLYLHYGKQYLKDCY+EG
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHYGKQYLKDCYVEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR
Sbjct: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL
Sbjct: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA
Sbjct: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 318

[Close]

BLAST of Carg02235 vs. ExPASy Swiss-Prot
Match: Q9LVQ0 (Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 5.8e-154
Identity = 248/333 (74.47%), Postives = 282/333 (84.68%), Query Frame = 0


Query: 3   APPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAG 62
           A  R++ V+QDG GD+ +VQ+AID+VP  NTCRTVIR+SPGIYRQP+YVPK KNFITFAG
Sbjct: 2   ATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAG 61

Query: 63  LNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQ 122
           ++PE TVLTW+NTA+KI HHQASRVIGTGTFGCGS IVEGEDFIAENITFENS+P+GSGQ
Sbjct: 62  ISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQ 121

Query: 123 AVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEGSV 182
           AVAIRVTADRCAFYNCRFLGW                 QDTLYLH+GKQYLKDCYIEGSV
Sbjct: 122 AVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHHGKQYLKDCYIEGSV 181

Query: 183 DFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPW 242
           DFIFGNSTALLE CHIHCKS GFITAQSRKSSQE+TGYVFLRCVITG+  + Y++LGRPW
Sbjct: 182 DFIFGNSTALLEHCHIHCKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPW 241

Query: 243 GPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVD 302
           GPF RVV AYTYMDACI+  GW NWG  ENER+ACFYEY+CFGPGSCS  RV W+REL+D
Sbjct: 242 GPFGRVVLAYTYMDACIRNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMD 301

Query: 303 EEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           +EA  F+ H F+DP+ +RPWLC RM ++ P+SA
Sbjct: 302 DEAGHFVHHSFVDPEQDRPWLCLRMGVKTPYSA 317

[Close]

BLAST of Carg02235 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.0e-70
Identity = 145/320 (45.31%), Postives = 184/320 (57.50%), Query Frame = 0


Query: 4   PPRVLTV-AQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAG 63
           P   LTV  +   GDF  +Q+AID++P  N  R VI+V  G+Y++ + +P  K FIT  G
Sbjct: 81  PSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEG 140

Query: 64  LNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP----- 123
              E T + W +TA        S+    GT+   S  V    F+A+NITF N++P     
Sbjct: 141 EGAEKTTVEWGDTA----QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPG 200

Query: 124 QGSGQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCY 183
               QAVA+RV+AD  AF+ CR LG                  QDTLY H G+ Y KDCY
Sbjct: 201 AVGKQAVALRVSADNAAFFGCRMLG-----------------AQDTLYDHLGRHYYKDCY 260

Query: 184 IEGSVDFIFGNSTALLERCHIHCKSH--GFITAQSRKSSQETTGYVFLRCVITGSAGTSY 243
           IEGSVDFIFGN+ +L E CH+H  +   G +TAQ R S  E TG+ F++C +T   GT  
Sbjct: 261 IEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVT---GTGV 320

Query: 244 VHLGRPWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVC 303
           ++LGR WGPF+RVVFAYTYMD  I P GW NWG    E T  + +YKC G G+    RV 
Sbjct: 321 LYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVA 376

Query: 304 WARELVDEEADEFILHQFID 316
           WAREL DEEA  F+   FID
Sbjct: 381 WARELTDEEAKPFLSLTFID 376

[Close]

BLAST of Carg02235 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.2e-57
Identity = 121/312 (38.78%), Postives = 173/312 (55.45%), Query Frame = 0


Query: 7   VLTVAQDGCGDFRTVQEAIDAVP--FSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLN 66
           ++ V Q G GDF  +QEAI+++P   +N+    I V PGIYR+ + +P  K +IT +G  
Sbjct: 50  LIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQ 109

Query: 67  PETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQAV 126
              T L W +              G       +  +   DF+   +T +N     +G+AV
Sbjct: 110 ASNTFLIWSD--------------GEDILESPTLTIFASDFVCRFLTIQNKFGT-AGRAV 169

Query: 127 AIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEGSVDF 186
           A+RV AD+ AFY C         +++S         QDTL    G  Y K+CYIEG+ DF
Sbjct: 170 ALRVAADKAAFYGC---------VITS--------YQDTLLDDNGNHYFKNCYIEGATDF 229

Query: 187 IFGNSTALLERCHIHCKS--HGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPW 246
           I G++++L ERCH+H  S  +G ITAQ R S+ E +G+ FL C +TGS  T    LGRPW
Sbjct: 230 ICGSASSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGST---FLGRPW 289

Query: 247 GPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVD 306
           G ++RVVFAY++    + P GW+ WG +  E T  + EYKC+GPG+    RV W+++L D
Sbjct: 290 GAYSRVVFAYSFFSNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSD 326

Query: 307 EEADEFILHQFI 315
           EEA  F+   FI
Sbjct: 350 EEATVFLSKDFI 326

[Close]

BLAST of Carg02235 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 121/332 (36.45%), Postives = 179/332 (53.92%), Query Frame = 0


Query: 6   RVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLNP 65
           +V+TV+ +G   FR+VQ+A+D++P +N     I+++PG YR+ + VP TK +ITF G   
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 66  ETTVLTWDNTATKI-SHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QG 125
           + T + W + A+ + ++ Q  R     T+   S  V    F A NI+F N++P       
Sbjct: 119 DVTAIEWHDRASDLGANGQQLR-----TYQTASVTVYANYFTARNISFTNTAPAPLPGMQ 178

Query: 126 SGQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIE 185
             QAVA R++ D+  F  C F G                  QDTL    G+ Y K+CYIE
Sbjct: 179 GWQAVAFRISGDKAFFSGCGFYG-----------------AQDTLCDDAGRHYFKECYIE 238

Query: 186 GSVDFIFGNSTALLERCHIH--CKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVH 245
           GS+DFIFGN  ++ + C +H      G I A  R   +E TG+ F+ C +T   GT  ++
Sbjct: 239 GSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVT---GTGPLY 298

Query: 246 LGRPWGPFARVVFAYTYMDACIKPAGWDNWG-KTENERTACFYEYKCFGPGSCSMSRVCW 305
           +GR  G ++R+V+AYTY DA +   GWD+W  K+   +TA F  Y C+GPG+ +   V W
Sbjct: 299 VGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSW 358

Query: 306 ARELVDEEADEFILHQFIDPDVERPWLCRRMA 329
           AR L  E A  FI   F++    R W+  R A
Sbjct: 359 ARALDYESAHPFIAKSFVN---GRHWIAPRDA 362

[Close]

BLAST of Carg02235 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.2e-56
Identity = 123/323 (38.08%), Postives = 174/323 (53.87%), Query Frame = 0


Query: 7   VLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLNPE 66
           VLTV   GCG+F  VQ AID VP  ++ +T+I V+ G YR+ + V + K  +   G   +
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQ 149

Query: 67  TTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSG 126
            T + W++TA    +          T    S +V   +F A NI+F+N++P     +   
Sbjct: 150 NTSIEWNDTAKSAGN----------TADSFSFVVFAANFTAYNISFKNNAPEPDPGEADA 209

Query: 127 QAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEGS 186
           QAVA+R+  D+ AFY C F G                  QDTL    G+ + K+C+I+GS
Sbjct: 210 QAVALRIEGDQAAFYGCGFYG-----------------AQDTLLDDKGRHFFKECFIQGS 269

Query: 187 VDFIFGNSTALLERCHIHCKSH-------GFITAQSRKSSQETTGYVFLRCVITGSAGTS 246
           +DFIFGN  +L + C I+  +        G ITAQ R+S  E +G+ F+ C I GS    
Sbjct: 270 IDFIFGNGRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGS---G 329

Query: 247 YVHLGRPWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRV 306
            + LGR WG +A VVF+ TYM   I P GW+NWG +  E+T  F E+KC+GPG+    RV
Sbjct: 330 EILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERV 382

Query: 307 CWARELVDEEADEFILHQFIDPD 318
            + ++L D EA  FI   FID D
Sbjct: 390 LFGKQLTDSEASSFIDVSFIDGD 382

[Close]

BLAST of Carg02235 vs. ExPASy TrEMBL
Match: A0A6J1FE78 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444715 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.5e-189
Identity = 317/335 (94.63%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNCRFLGW                 QDTLYLHYGKQYLKDCYIEG
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHYGKQYLKDCYIEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLERCH+HCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR
Sbjct: 181 SVDFIFGNSTALLERCHVHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL
Sbjct: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA
Sbjct: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 318

[Close]

BLAST of Carg02235 vs. ExPASy TrEMBL
Match: A0A6J1JZ39 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489575 PE=4 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.3e-188
Identity = 316/335 (94.33%), Postives = 317/335 (94.63%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNCRFLGW                 QDTLYLHYGKQYLKDCYIEG
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHYGKQYLKDCYIEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAG+SYVHLGR
Sbjct: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGSSYVHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSC MSRVCWAREL
Sbjct: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCPMSRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA
Sbjct: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 318

[Close]

BLAST of Carg02235 vs. ExPASy TrEMBL
Match: A0A6J1BZD5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111006628 PE=4 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 6.3e-180
Identity = 297/335 (88.66%), Postives = 308/335 (91.94%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAAPPRV+TVA DGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF
Sbjct: 1   MAAPPRVITVAHDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGL+PE+TVLTWDNTATKI+HHQASR+IGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLSPESTVLTWDNTATKINHHQASRIIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNC+FLGW                 QDTLYLHYGKQYLKDCYIEG
Sbjct: 121 GQAVAIRVTADRCAFYNCKFLGW-----------------QDTLYLHYGKQYLKDCYIEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLE CH+HCKS GFITAQSRKSSQETTGYVFLRCVITGS G SY+HLGR
Sbjct: 181 SVDFIFGNSTALLEHCHVHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGNSYIHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYTYMDACIKPAGWDNWGK ENERTACFYEYKCFGPGSC M RVCWAREL
Sbjct: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKIENERTACFYEYKCFGPGSCQMKRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           VDEE DEF+LHQFIDP+VERPWLC+RMA RIPFSA
Sbjct: 301 VDEETDEFLLHQFIDPNVERPWLCQRMASRIPFSA 318

[Close]

BLAST of Carg02235 vs. ExPASy TrEMBL
Match: A0A5A7TBP8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002680 PE=4 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 4.5e-178
Identity = 295/335 (88.06%), Postives = 306/335 (91.34%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRVLTVA DGC DFRTVQEAIDAVPFSNTCRTVIRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFRTVQEAIDAVPFSNTCRTVIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLNPETT+LTWDNTAT+I HHQA+RVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATEIKHHQAARVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNCRFLGW                 QDTLYLHYG+QYLKDCYIEG
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHYGRQYLKDCYIEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLE CH+HCKS GFITAQSRKSSQETTGYVFLRCVITGS  TSYVHLGR
Sbjct: 181 SVDFIFGNSTALLEHCHVHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYT+MD CIKP GWDNWGK ENERTACFYEYKCFGPGSCSM RVCWAREL
Sbjct: 241 PWGPFARVVFAYTHMDVCIKPTGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           +DEEADEFILH+FIDPDV+RPWLC+RMALRIPFSA
Sbjct: 301 LDEEADEFILHRFIDPDVDRPWLCQRMALRIPFSA 318

[Close]

BLAST of Carg02235 vs. ExPASy TrEMBL
Match: A0A0A0KVN5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G637720 PE=4 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 7.7e-178
Identity = 294/335 (87.76%), Postives = 307/335 (91.64%), Query Frame = 0


Query: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60
           MAA PRVLTVA DGC DF TVQEAIDAVPFSNTCRT+IRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120
           AGLNPETT+LTWDNTATKI+HHQA+RVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEG 180
           GQAVAIRVTADRCAFYNCRFLGW                 QDTLYLHYG+QYLKDCYIEG
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHYGRQYLKDCYIEG 180

Query: 181 SVDFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGR 240
           SVDFIFGNSTALLE CH+HCKS GFITAQSRKSSQETTGYVFLRCVITGS  TSYVHLGR
Sbjct: 181 SVDFIFGNSTALLEHCHVHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGR 240

Query: 241 PWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWAREL 300
           PWGPFARVVFAYT+MD CIKPAGWDNWGK ENERTACFYEYKCFGPGSCSM RVCWAREL
Sbjct: 241 PWGPFARVVFAYTHMDVCIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWAREL 300

Query: 301 VDEEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           +DEEADEFILH+FIDPDV+RPWLC+RMALRIPFSA
Sbjct: 301 LDEEADEFILHRFIDPDVDRPWLCQRMALRIPFSA 318

[Close]

BLAST of Carg02235 vs. TAIR 10
Match: AT3G29090.1 (pectin methylesterase 31 )

HSP 1 Score: 545.0 bits (1403), Expect = 4.1e-155
Identity = 248/333 (74.47%), Postives = 282/333 (84.68%), Query Frame = 0


Query: 3   APPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAG 62
           A  R++ V+QDG GD+ +VQ+AID+VP  NTCRTVIR+SPGIYRQP+YVPK KNFITFAG
Sbjct: 2   ATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAG 61

Query: 63  LNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQ 122
           ++PE TVLTW+NTA+KI HHQASRVIGTGTFGCGS IVEGEDFIAENITFENS+P+GSGQ
Sbjct: 62  ISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQ 121

Query: 123 AVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEGSV 182
           AVAIRVTADRCAFYNCRFLGW                 QDTLYLH+GKQYLKDCYIEGSV
Sbjct: 122 AVAIRVTADRCAFYNCRFLGW-----------------QDTLYLHHGKQYLKDCYIEGSV 181

Query: 183 DFIFGNSTALLERCHIHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPW 242
           DFIFGNSTALLE CHIHCKS GFITAQSRKSSQE+TGYVFLRCVITG+  + Y++LGRPW
Sbjct: 182 DFIFGNSTALLEHCHIHCKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPW 241

Query: 243 GPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVD 302
           GPF RVV AYTYMDACI+  GW NWG  ENER+ACFYEY+CFGPGSCS  RV W+REL+D
Sbjct: 242 GPFGRVVLAYTYMDACIRNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMD 301

Query: 303 EEADEFILHQFIDPDVERPWLCRRMALRIPFSA 336
           +EA  F+ H F+DP+ +RPWLC RM ++ P+SA
Sbjct: 302 DEAGHFVHHSFVDPEQDRPWLCLRMGVKTPYSA 317

[Close]

BLAST of Carg02235 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.1e-71
Identity = 145/320 (45.31%), Postives = 184/320 (57.50%), Query Frame = 0


Query: 4   PPRVLTV-AQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAG 63
           P   LTV  +   GDF  +Q+AID++P  N  R VI+V  G+Y++ + +P  K FIT  G
Sbjct: 81  PSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEG 140

Query: 64  LNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP----- 123
              E T + W +TA        S+    GT+   S  V    F+A+NITF N++P     
Sbjct: 141 EGAEKTTVEWGDTA----QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPG 200

Query: 124 QGSGQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCY 183
               QAVA+RV+AD  AF+ CR LG                  QDTLY H G+ Y KDCY
Sbjct: 201 AVGKQAVALRVSADNAAFFGCRMLG-----------------AQDTLYDHLGRHYYKDCY 260

Query: 184 IEGSVDFIFGNSTALLERCHIHCKSH--GFITAQSRKSSQETTGYVFLRCVITGSAGTSY 243
           IEGSVDFIFGN+ +L E CH+H  +   G +TAQ R S  E TG+ F++C +T   GT  
Sbjct: 261 IEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVT---GTGV 320

Query: 244 VHLGRPWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVC 303
           ++LGR WGPF+RVVFAYTYMD  I P GW NWG    E T  + +YKC G G+    RV 
Sbjct: 321 LYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVA 376

Query: 304 WARELVDEEADEFILHQFID 316
           WAREL DEEA  F+   FID
Sbjct: 381 WARELTDEEAKPFLSLTFID 376

[Close]

BLAST of Carg02235 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 224.2 bits (570), Expect = 1.6e-58
Identity = 121/312 (38.78%), Postives = 173/312 (55.45%), Query Frame = 0


Query: 7   VLTVAQDGCGDFRTVQEAIDAVP--FSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLN 66
           ++ V Q G GDF  +QEAI+++P   +N+    I V PGIYR+ + +P  K +IT +G  
Sbjct: 50  LIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQ 109

Query: 67  PETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGSGQAV 126
              T L W +              G       +  +   DF+   +T +N     +G+AV
Sbjct: 110 ASNTFLIWSD--------------GEDILESPTLTIFASDFVCRFLTIQNKFGT-AGRAV 169

Query: 127 AIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEGSVDF 186
           A+RV AD+ AFY C         +++S         QDTL    G  Y K+CYIEG+ DF
Sbjct: 170 ALRVAADKAAFYGC---------VITS--------YQDTLLDDNGNHYFKNCYIEGATDF 229

Query: 187 IFGNSTALLERCHIHCKS--HGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPW 246
           I G++++L ERCH+H  S  +G ITAQ R S+ E +G+ FL C +TGS  T    LGRPW
Sbjct: 230 ICGSASSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGST---FLGRPW 289

Query: 247 GPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVD 306
           G ++RVVFAY++    + P GW+ WG +  E T  + EYKC+GPG+    RV W+++L D
Sbjct: 290 GAYSRVVFAYSFFSNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSD 326

Query: 307 EEADEFILHQFI 315
           EEA  F+   FI
Sbjct: 350 EEATVFLSKDFI 326

[Close]

BLAST of Carg02235 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.4e-57
Identity = 121/332 (36.45%), Postives = 179/332 (53.92%), Query Frame = 0


Query: 6   RVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLNP 65
           +V+TV+ +G   FR+VQ+A+D++P +N     I+++PG YR+ + VP TK +ITF G   
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 66  ETTVLTWDNTATKI-SHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QG 125
           + T + W + A+ + ++ Q  R     T+   S  V    F A NI+F N++P       
Sbjct: 119 DVTAIEWHDRASDLGANGQQLR-----TYQTASVTVYANYFTARNISFTNTAPAPLPGMQ 178

Query: 126 SGQAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIE 185
             QAVA R++ D+  F  C F G                  QDTL    G+ Y K+CYIE
Sbjct: 179 GWQAVAFRISGDKAFFSGCGFYG-----------------AQDTLCDDAGRHYFKECYIE 238

Query: 186 GSVDFIFGNSTALLERCHIH--CKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVH 245
           GS+DFIFGN  ++ + C +H      G I A  R   +E TG+ F+ C +T   GT  ++
Sbjct: 239 GSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVT---GTGPLY 298

Query: 246 LGRPWGPFARVVFAYTYMDACIKPAGWDNWG-KTENERTACFYEYKCFGPGSCSMSRVCW 305
           +GR  G ++R+V+AYTY DA +   GWD+W  K+   +TA F  Y C+GPG+ +   V W
Sbjct: 299 VGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSW 358

Query: 306 ARELVDEEADEFILHQFIDPDVERPWLCRRMA 329
           AR L  E A  FI   F++    R W+  R A
Sbjct: 359 ARALDYESAHPFIAKSFVN---GRHWIAPRDA 362

[Close]

BLAST of Carg02235 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 220.3 bits (560), Expect = 2.3e-57
Identity = 123/323 (38.08%), Postives = 174/323 (53.87%), Query Frame = 0


Query: 7   VLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITFAGLNPE 66
           VLTV   GCG+F  VQ AID VP  ++ +T+I V+ G YR+ + V + K  +   G   +
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQ 149

Query: 67  TTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSP-----QGSG 126
            T + W++TA    +          T    S +V   +F A NI+F+N++P     +   
Sbjct: 150 NTSIEWNDTAKSAGN----------TADSFSFVVFAANFTAYNISFKNNAPEPDPGEADA 209

Query: 127 QAVAIRVTADRCAFYNCRFLGWQPAAIVSSSIIFLDNLMQDTLYLHYGKQYLKDCYIEGS 186
           QAVA+R+  D+ AFY C F G                  QDTL    G+ + K+C+I+GS
Sbjct: 210 QAVALRIEGDQAAFYGCGFYG-----------------AQDTLLDDKGRHFFKECFIQGS 269

Query: 187 VDFIFGNSTALLERCHIHCKSH-------GFITAQSRKSSQETTGYVFLRCVITGSAGTS 246
           +DFIFGN  +L + C I+  +        G ITAQ R+S  E +G+ F+ C I GS    
Sbjct: 270 IDFIFGNGRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGS---G 329

Query: 247 YVHLGRPWGPFARVVFAYTYMDACIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRV 306
            + LGR WG +A VVF+ TYM   I P GW+NWG +  E+T  F E+KC+GPG+    RV
Sbjct: 330 EILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERV 382

Query: 307 CWARELVDEEADEFILHQFIDPD 318
            + ++L D EA  FI   FID D
Sbjct: 390 LFGKQLTDSEASSFIDVSFIDGD 382

The following BLAST results are available for this feature:

BLAST of Carg02235 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016123.1	7.1e-202	100.00	Pectinesterase 31 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6578565.1	2.4e-189	94.93	Pectinesterase 31, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022938497.1	3.1e-189	94.63	pectinesterase 31 [Cucurbita moschata]	[more]
XP_022993645.1	2.6e-188	94.33	pectinesterase 31 [Cucurbita maxima]	[more]
XP_023551220.1	1.3e-187	94.03	pectinesterase 31 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023551221.1 pectin...	[more]

BLAST of Carg02235 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVQ0	5.8e-154	74.47	Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1	[more]
Q8VYZ3	3.0e-70	45.31	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q9SIJ9	2.2e-57	38.78	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]
Q8LPF3	1.9e-56	36.45	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9ZQA3	3.2e-56	38.08	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]

BLAST of Carg02235 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FE78	1.5e-189	94.63	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444715 PE=4 SV=1	[more]
A0A6J1JZ39	1.3e-188	94.33	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489575 PE=4 SV=1	[more]
A0A6J1BZD5	6.3e-180	88.66	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111006628 PE=4 SV=1	[more]
A0A5A7TBP8	4.5e-178	88.06	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00268...	[more]
A0A0A0KVN5	7.7e-178	87.76	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G637720 PE=4 SV=1	[more]

BLAST of Carg02235 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29090.1	4.1e-155	74.47	pectin methylesterase 31	[more]
AT5G19730.1	2.1e-71	45.31	Pectin lyase-like superfamily protein	[more]
AT2G21610.1	1.6e-58	38.78	pectinesterase 11	[more]
AT5G47500.1	1.4e-57	36.45	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	2.3e-57	38.08	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 9..315 e-value: 5.2E-66 score: 222.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 5..325 e-value: 3.5E-102 score: 344.0
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 6..334
None	No IPR available	PANTHER	PTHR31321:SF71	PECTINESTERASE	coord: 6..334
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 178..187
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..322

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02235-RA	Carg02235-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0050829	defense response to Gram-negative bacterium
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity