Carg02226 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg02226 vs. NCBI nr
Match: KAG7016114.1 (Protein TPX2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1037.3 bits (2681), Expect = 4.6e-299
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK
Sbjct: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC
Sbjct: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE
Sbjct: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
           AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS
Sbjct: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240

Query: 241 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALDPA 300
           KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALDPA
Sbjct: 241 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALDPA 300

Query: 301 ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ 360
           ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ
Sbjct: 301 ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ 360

Query: 361 RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRCLV 420
           RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRCLV
Sbjct: 361 RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRCLV 420

Query: 421 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 480
           KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL
Sbjct: 421 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 480

Query: 481 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYTF 540
           RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYTF
Sbjct: 481 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYTF 540

Query: 541 QP 543
           QP
Sbjct: 541 QP 542

BLAST of Carg02226 vs. NCBI nr
Match: KAG6578554.1 (Protein TPX2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 974.9 bits (2519), Expect = 2.8e-280
Identity = 520/542 (95.94%), Postives = 521/542 (96.13%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK
Sbjct: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC
Sbjct: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE
Sbjct: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
           AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS
Sbjct: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240

Query: 241 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALDPA 300
           KDSDE SENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSS CPSELFFTSENRALDPA
Sbjct: 241 KDSDEQSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSFCPSELFFTSENRALDPA 300

Query: 301 ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ 360
           ASVSSSWD SRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ
Sbjct: 301 ASVSSSWDISRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ 360

Query: 361 RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRCLV 420
           RGKEKG+EFLKRVQETMMEQERQRIPIAQGLPWTTDEPE                  CLV
Sbjct: 361 RGKEKGQEFLKRVQETMMEQERQRIPIAQGLPWTTDEPE------------------CLV 420

Query: 421 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 480
           KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL
Sbjct: 421 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 480

Query: 481 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYTF 540
           RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYTF
Sbjct: 481 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYTF 524

Query: 541 QP 543
           QP
Sbjct: 541 QP 524

BLAST of Carg02226 vs. NCBI nr
Match: XP_022938982.1 (protein WVD2-like 4 [Cucurbita moschata])

HSP 1 Score: 971.5 bits (2510), Expect = 3.1e-279
Identity = 519/542 (95.76%), Postives = 520/542 (95.94%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGT+TPVKDSTGSGLKMIQEVS NSENTNPNVNDSISSVPKLWKSSPSMKSAK
Sbjct: 42  MGSIGKNGGTVTPVKDSTGSGLKMIQEVSENSENTNPNVNDSISSVPKLWKSSPSMKSAK 101

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC
Sbjct: 102 TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 161

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE
Sbjct: 162 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 221

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
           AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS
Sbjct: 222 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 281

Query: 241 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALDPA 300
           KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSS CPSELFFTSENRALDPA
Sbjct: 282 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSFCPSELFFTSENRALDPA 341

Query: 301 ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ 360
           ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVT QKPFKLRTEQ
Sbjct: 342 ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTGQKPFKLRTEQ 401

Query: 361 RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRCLV 420
           RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPE                  CLV
Sbjct: 402 RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPE------------------CLV 461

Query: 421 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 480
           KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL
Sbjct: 462 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 521

Query: 481 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYTF 540
           RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHY F
Sbjct: 522 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYAF 565

Query: 541 QP 543
           QP
Sbjct: 582 QP 565

BLAST of Carg02226 vs. NCBI nr
Match: XP_023549623.1 (protein WVD2-like 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 950.7 bits (2456), Expect = 5.6e-273
Identity = 511/542 (94.28%), Postives = 515/542 (95.02%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGT+TPVKDSTGSG KMIQEVSGNSENTNPNVNDSISSV KLWKSSPSMKS K
Sbjct: 1   MGSIGKNGGTVTPVKDSTGSGSKMIQEVSGNSENTNPNVNDSISSVLKLWKSSPSMKSTK 60

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC
Sbjct: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDR E
Sbjct: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRTE 180

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
           AEAFDVNGESESPEQVS+S+IKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS
Sbjct: 181 AEAFDVNGESESPEQVSNSMIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240

Query: 241 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALDPA 300
           KDSDE  ENQEKAMEEKNK+IKWALPGLPPPKVSEMQGSSSS  PSELFFTSENRALDPA
Sbjct: 241 KDSDEQPENQEKAMEEKNKLIKWALPGLPPPKVSEMQGSSSSFFPSELFFTSENRALDPA 300

Query: 301 ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ 360
           ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ
Sbjct: 301 ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ 360

Query: 361 RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRCLV 420
           RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPE                  CLV
Sbjct: 361 RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPE------------------CLV 420

Query: 421 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 480
           KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL
Sbjct: 421 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 480

Query: 481 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYTF 540
           RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGS HHYTF
Sbjct: 481 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGS-HHYTF 523

Query: 541 QP 543
           QP
Sbjct: 541 QP 523

BLAST of Carg02226 vs. NCBI nr
Match: XP_022993562.1 (uncharacterized protein LOC111489526 isoform X1 [Cucurbita maxima] >XP_022993563.1 uncharacterized protein LOC111489526 isoform X1 [Cucurbita maxima])

HSP 1 Score: 932.6 bits (2409), Expect = 1.6e-267
Identity = 504/544 (92.65%), Postives = 512/544 (94.12%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGT+TPVKDSTGSG KMIQEVSGNSENTNPNVN+SIS VPKLWKSSPSMKSAK
Sbjct: 42  MGSIGKNGGTVTPVKDSTGSGSKMIQEVSGNSENTNPNVNNSISPVPKLWKSSPSMKSAK 101

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCE NTRSDAKKCLC
Sbjct: 102 TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEDNTRSDAKKCLC 161

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE
Sbjct: 162 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 221

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
           AEAFDVNGESESPEQVS+S+IKRRRERLLE ARNSVPETGSGKVKHLVMAFEKL+TIRVS
Sbjct: 222 AEAFDVNGESESPEQVSNSMIKRRRERLLEAARNSVPETGSGKVKHLVMAFEKLKTIRVS 281

Query: 241 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALD-- 300
           KDSDE SENQEKAMEEKNKVIKWALPGLPPPKVSEMQ SSSS CPSELFFTSEN ALD  
Sbjct: 282 KDSDEQSENQEKAMEEKNKVIKWALPGLPPPKVSEMQASSSSFCPSELFFTSENLALDLP 341

Query: 301 PAASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRT 360
            + SVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRT
Sbjct: 342 VSVSVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRT 401

Query: 361 EQRGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRC 420
           EQRGKEKGEEFLKRVQETM+EQERQRIPIAQGLPWTTDEPE                  C
Sbjct: 402 EQRGKEKGEEFLKRVQETMIEQERQRIPIAQGLPWTTDEPE------------------C 461

Query: 421 LVKPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR 480
           LVKPPVK NTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR
Sbjct: 462 LVKPPVKGNTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR 521

Query: 481 RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHY 540
           RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGS H+Y
Sbjct: 522 RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGS-HYY 566

Query: 541 TFQP 543
           TFQP
Sbjct: 582 TFQP 566

BLAST of Carg02226 vs. ExPASy Swiss-Prot
Match: F4I2H7 (Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.1e-12
Identity = 86/339 (25.37%), Postives = 152/339 (44.84%), Query Frame = 0

Query: 239 VSKDSDELSENQ-EKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLC--------PSELF 298
           ++K + EL + + EKA + K K +          K+ E +G     C        P E  
Sbjct: 435 IAKTTAELEQEELEKAPKFKAKPLN--------KKIFESKGEMGIFCNTKKHITIPQEFH 494

Query: 299 FTSENRALDPAASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVT 358
           F ++ R   P  SV   +D  + S++S + H                     +K   + T
Sbjct: 495 FATDERISRP-ESVLDIFD--KLSLNSESCH---------------------EKPLPRNT 554

Query: 359 RQKPFKLRTEQRGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTD--------EPEQG 418
              PF L+TE+RG EK ++F   +    +   + R+P A   P+TTD        EP+Q 
Sbjct: 555 APNPFNLKTEERGAEKEKKFYMELMYKKLGDVKARVPKANPYPYTTDYPVVPPKPEPKQC 614

Query: 419 RWP----LGGWI---------------LRSHVGWRCLVK--PPVKENTRPV--------- 478
             P    L   +               + +    + L K  P +KE+  PV         
Sbjct: 615 TQPEPFQLESLVRHEEEMRREREERRRMETEEAQKRLFKAQPVIKEDPIPVPEKVRMPLT 674

Query: 479 ---DLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRLRKELVPKA 528
              +  LH + RAVERA+FD+++ EK +  ++Y+ + E  + + EE  L+++RK +VP A
Sbjct: 675 EIQEFNLHVEHRAVERADFDHKIKEKENQYKRYREESEAAKMVEEERALKQMRKTMVPHA 734

BLAST of Carg02226 vs. ExPASy TrEMBL
Match: A0A6J1FFJ6 (protein WVD2-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111445027 PE=3 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.5e-279
Identity = 519/542 (95.76%), Postives = 520/542 (95.94%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGT+TPVKDSTGSGLKMIQEVS NSENTNPNVNDSISSVPKLWKSSPSMKSAK
Sbjct: 42  MGSIGKNGGTVTPVKDSTGSGLKMIQEVSENSENTNPNVNDSISSVPKLWKSSPSMKSAK 101

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC
Sbjct: 102 TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 161

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE
Sbjct: 162 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 221

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
           AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS
Sbjct: 222 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 281

Query: 241 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALDPA 300
           KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSS CPSELFFTSENRALDPA
Sbjct: 282 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSFCPSELFFTSENRALDPA 341

Query: 301 ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQ 360
           ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVT QKPFKLRTEQ
Sbjct: 342 ASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTGQKPFKLRTEQ 401

Query: 361 RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRCLV 420
           RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPE                  CLV
Sbjct: 402 RGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPE------------------CLV 461

Query: 421 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 480
           KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL
Sbjct: 462 KPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRL 521

Query: 481 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYTF 540
           RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHY F
Sbjct: 522 RKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHYAF 565

Query: 541 QP 543
           QP
Sbjct: 582 QP 565

BLAST of Carg02226 vs. ExPASy TrEMBL
Match: A0A6J1K2M1 (uncharacterized protein LOC111489526 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489526 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.7e-268
Identity = 504/544 (92.65%), Postives = 512/544 (94.12%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGT+TPVKDSTGSG KMIQEVSGNSENTNPNVN+SIS VPKLWKSSPSMKSAK
Sbjct: 42  MGSIGKNGGTVTPVKDSTGSGSKMIQEVSGNSENTNPNVNNSISPVPKLWKSSPSMKSAK 101

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCE NTRSDAKKCLC
Sbjct: 102 TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEDNTRSDAKKCLC 161

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE
Sbjct: 162 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 221

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
           AEAFDVNGESESPEQVS+S+IKRRRERLLE ARNSVPETGSGKVKHLVMAFEKL+TIRVS
Sbjct: 222 AEAFDVNGESESPEQVSNSMIKRRRERLLEAARNSVPETGSGKVKHLVMAFEKLKTIRVS 281

Query: 241 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALD-- 300
           KDSDE SENQEKAMEEKNKVIKWALPGLPPPKVSEMQ SSSS CPSELFFTSEN ALD  
Sbjct: 282 KDSDEQSENQEKAMEEKNKVIKWALPGLPPPKVSEMQASSSSFCPSELFFTSENLALDLP 341

Query: 301 PAASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRT 360
            + SVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRT
Sbjct: 342 VSVSVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRT 401

Query: 361 EQRGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRC 420
           EQRGKEKGEEFLKRVQETM+EQERQRIPIAQGLPWTTDEPE                  C
Sbjct: 402 EQRGKEKGEEFLKRVQETMIEQERQRIPIAQGLPWTTDEPE------------------C 461

Query: 421 LVKPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR 480
           LVKPPVK NTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR
Sbjct: 462 LVKPPVKGNTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR 521

Query: 481 RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHY 540
           RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGS H+Y
Sbjct: 522 RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGS-HYY 566

Query: 541 TFQP 543
           TFQP
Sbjct: 582 TFQP 566

BLAST of Carg02226 vs. ExPASy TrEMBL
Match: A0A6J1K0I7 (uncharacterized protein LOC111489526 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489526 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.7e-268
Identity = 504/544 (92.65%), Postives = 512/544 (94.12%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGT+TPVKDSTGSG KMIQEVSGNSENTNPNVN+SIS VPKLWKSSPSMKSAK
Sbjct: 1   MGSIGKNGGTVTPVKDSTGSGSKMIQEVSGNSENTNPNVNNSISPVPKLWKSSPSMKSAK 60

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCE NTRSDAKKCLC
Sbjct: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEDNTRSDAKKCLC 120

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE
Sbjct: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
           AEAFDVNGESESPEQVS+S+IKRRRERLLE ARNSVPETGSGKVKHLVMAFEKL+TIRVS
Sbjct: 181 AEAFDVNGESESPEQVSNSMIKRRRERLLEAARNSVPETGSGKVKHLVMAFEKLKTIRVS 240

Query: 241 KDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENRALD-- 300
           KDSDE SENQEKAMEEKNKVIKWALPGLPPPKVSEMQ SSSS CPSELFFTSEN ALD  
Sbjct: 241 KDSDEQSENQEKAMEEKNKVIKWALPGLPPPKVSEMQASSSSFCPSELFFTSENLALDLP 300

Query: 301 PAASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRT 360
            + SVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRT
Sbjct: 301 VSVSVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRT 360

Query: 361 EQRGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRC 420
           EQRGKEKGEEFLKRVQETM+EQERQRIPIAQGLPWTTDEPE                  C
Sbjct: 361 EQRGKEKGEEFLKRVQETMIEQERQRIPIAQGLPWTTDEPE------------------C 420

Query: 421 LVKPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR 480
           LVKPPVK NTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR
Sbjct: 421 LVKPPVKGNTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR 480

Query: 481 RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHY 540
           RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGS H+Y
Sbjct: 481 RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGS-HYY 525

Query: 541 TFQP 543
           TFQP
Sbjct: 541 TFQP 525

BLAST of Carg02226 vs. ExPASy TrEMBL
Match: A0A1S3CAJ5 (uncharacterized protein LOC103498525 OS=Cucumis melo OX=3656 GN=LOC103498525 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 3.7e-222
Identity = 436/546 (79.85%), Postives = 466/546 (85.35%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGT+TPVK+STGS  KMIQEVS +SEN NPNVND+IS VPKL KSSP MKSAK
Sbjct: 1   MGSIGKNGGTVTPVKESTGSRSKMIQEVSRHSENMNPNVNDTISPVPKLSKSSPLMKSAK 60

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           TAQKSAA+NPS VLNSPKYKIRERKF+VAKKN K EKSGK KTDCKCEV   SD+KKCLC
Sbjct: 61  TAQKSAARNPSVVLNSPKYKIRERKFLVAKKNQKKEKSGKSKTDCKCEVRNGSDSKKCLC 120

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQ+GG GE          IRAEI+LEEEMERKLMIQDLQNEDR E
Sbjct: 121 LAYETLRASQEEFFKNQNGGTGE----------IRAEIELEEEMERKLMIQDLQNEDRVE 180

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
            E  DVNGESESPEQVSSS+IKRRRERLLETARNSVPETGSGKVKHLV AFEKLRTI+V+
Sbjct: 181 TENHDVNGESESPEQVSSSIIKRRRERLLETARNSVPETGSGKVKHLVKAFEKLRTIQVA 240

Query: 241 KD-----SDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENR 300
           K+      ++  ENQ+K +EEK+KV+KWALPGLPPPKV E   SSSS CPSELF TSEN 
Sbjct: 241 KEETDENEEKQLENQDKPIEEKSKVMKWALPGLPPPKVIE---SSSSFCPSELFLTSENL 300

Query: 301 ALDPAASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFK 360
            LD  ASVSSSWD SRGSISSR  +GGRRSRRNS ES GT+GGSRWKK+QLKVTRQKPFK
Sbjct: 301 GLDSRASVSSSWDGSRGSISSR--NGGRRSRRNSAESAGTIGGSRWKKKQLKVTRQKPFK 360

Query: 361 LRTEQRGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVG 420
           LRTEQRGKEK EEFLKRVQETM+E+ERQRIPIAQGLPWTTDEPE                
Sbjct: 361 LRTEQRGKEKEEEFLKRVQETMIEEERQRIPIAQGLPWTTDEPE---------------- 420

Query: 421 WRCLVKPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEE 480
             CLVKPPVKE T+PVDLVLHSDVRAVERAEFD+QVAEKLS+IEQYKM+REKQQKMAEEE
Sbjct: 421 --CLVKPPVKEITKPVDLVLHSDVRAVERAEFDHQVAEKLSVIEQYKMEREKQQKMAEEE 480

Query: 481 ELRRLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSH 540
           ELRRLRKELVPKAQPMPYFDRPFIPRRSAKLPTIP+EPKFHIPQQKK KSCL WND+   
Sbjct: 481 ELRRLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPKEPKFHIPQQKKIKSCLSWNDMS-- 510

Query: 541 HHYTFQ 542
            HYTFQ
Sbjct: 541 -HYTFQ 510

BLAST of Carg02226 vs. ExPASy TrEMBL
Match: A0A0A0KVS9 (TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G638410 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.8e-217
Identity = 425/547 (77.70%), Postives = 465/547 (85.01%), Query Frame = 0

Query: 1   MGSIGKNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAK 60
           MGSIGKNGGT+TPVK+STGS  KMIQEVS +SEN NPNVND+IS VPKL KSSP +KS K
Sbjct: 1   MGSIGKNGGTVTPVKESTGSRSKMIQEVSRHSENMNPNVNDTISPVPKLSKSSPLIKSTK 60

Query: 61  TAQKSAAKNPSAVLNSPKYKIRERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLC 120
           T QKSAA+NPS VLNSPKYKIRERKFVVAKKN K EK+GK KTDCKC+V   SD+KKCLC
Sbjct: 61  TVQKSAARNPSVVLNSPKYKIRERKFVVAKKNQKKEKTGKSKTDCKCDVGNGSDSKKCLC 120

Query: 121 LAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAE 180
           LAYETLRASQEEFFKNQ+GGIGE          IRAEI+LEEEMERKLMIQDLQNE+  E
Sbjct: 121 LAYETLRASQEEFFKNQNGGIGE----------IRAEIELEEEMERKLMIQDLQNEEGIE 180

Query: 181 AEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVS 240
            E  DVNGESESPEQVSSS+IKRRRERLLETARNSVPETGSGKVKHLV+AFEKLRTI+V+
Sbjct: 181 TENHDVNGESESPEQVSSSIIKRRRERLLETARNSVPETGSGKVKHLVLAFEKLRTIQVA 240

Query: 241 KD-----SDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFTSENR 300
           K+      ++  ENQ+K +EEK+KV+KWALPGLPPPKV E   SSSS CPSELF TSEN 
Sbjct: 241 KEETDENEEKQLENQDKPIEEKSKVMKWALPGLPPPKVIE---SSSSFCPSELFLTSENL 300

Query: 301 ALDPAASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFK 360
            LD  ASVSSSWD SRGSISSR  +GGRRSRRNS ES GT+GGS+WKK+QLKVTRQKPFK
Sbjct: 301 GLDSRASVSSSWDGSRGSISSR--NGGRRSRRNSAESAGTIGGSKWKKKQLKVTRQKPFK 360

Query: 361 LRTEQRGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVG 420
           LRTEQRGKEK EEFLKRVQETM+E+E+QRIPIAQGLPWTTDEPE                
Sbjct: 361 LRTEQRGKEKEEEFLKRVQETMIEEEKQRIPIAQGLPWTTDEPE---------------- 420

Query: 421 WRCLVKPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEE 480
             C+VKPPVKE TRPVDLVLHSDVRAVERAEFD+QVAEKLS+IEQYKM+REKQ+KMAEEE
Sbjct: 421 --CVVKPPVKEITRPVDLVLHSDVRAVERAEFDHQVAEKLSVIEQYKMEREKQEKMAEEE 480

Query: 481 ELRRLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSH 540
           EL+RLRKELVPKAQPMPYFDRPFIPRRS KLPTIP+EPKFHIPQQKK KSCL WND+ S 
Sbjct: 481 ELKRLRKELVPKAQPMPYFDRPFIPRRSGKLPTIPKEPKFHIPQQKKIKSCLSWNDM-SQ 513

Query: 541 HHYTFQP 543
           + + FQP
Sbjct: 541 YTFQFQP 513

BLAST of Carg02226 vs. TAIR 10
Match: AT5G15510.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 422.2 bits (1084), Expect = 6.5e-118
Identity = 288/545 (52.84%), Postives = 348/545 (63.85%), Query Frame = 0

Query: 6   KNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAKTAQKS 65
           KNG   TPVKD   S  +  Q++S +SEN NPN++ +  S       SP MKSAK   KS
Sbjct: 7   KNGKAGTPVKDR--SKFQSNQKISRSSENANPNISQASPS------KSPLMKSAK---KS 66

Query: 66  AAKNPS------AVLNSPKYKIRERKF-VVAKKNSKNEK-------SGKCKTDCKCEVNT 125
           A KNPS        + SP+ +IRERKF VVAKKNS+  K       S   + DCKC    
Sbjct: 67  AQKNPSPSPKPTQAVFSPRNRIRERKFVVVAKKNSRKGKKEPAITESKAAEIDCKCGERK 126

Query: 126 RSDAKKCLCLAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQ 185
           + +  KC+C+AYETLRASQEEFFKN+                    I+ EE+ E      
Sbjct: 127 KGN-MKCVCVAYETLRASQEEFFKNR--------------------IESEEDKEALEECC 186

Query: 186 DLQNEDRAEAEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAF 245
           +L N D  E +      ES  PE+   S +KR R +++E AR SVP   SGKV +LV AF
Sbjct: 187 NLGNGDELEGK------ESGEPEKTGVSTMKRSRAKVVEEARQSVPV--SGKVMNLVEAF 246

Query: 246 EKLRTIRVSKDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFT 305
           EKL     SK ++++ ENQ +  E+  K +K  L  L   K  E    SSS CPSE+  T
Sbjct: 247 EKLTCFSNSKTANKIEENQTE--EDTKKPVK--LEFLEGEK--EKNPWSSSFCPSEMVLT 306

Query: 306 SENRALDPAASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQ 365
           ++N  LDP AS+SSSWDS+RGS+ S +S+ GRRSRRNS ES  T+G  R KK+Q+KVT  
Sbjct: 307 AKNLGLDPNASISSSWDSTRGSVLSGSSNAGRRSRRNSMESSTTMGSRRSKKKQVKVTSL 366

Query: 366 KPFKLRTEQRGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILR 425
           KPFKLRTEQRGK K EE  K++ E  ME+E+ RIPIAQGLPWTTDEPE            
Sbjct: 367 KPFKLRTEQRGKVKEEELAKKIHEITMEEEKMRIPIAQGLPWTTDEPE------------ 426

Query: 426 SHVGWRCLVKPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKM 485
                 CLVKP  K+ TRPVDL LHSDVRAVERAEFD QVAEK+S IEQYKM+RE+QQK+
Sbjct: 427 ------CLVKPHWKDITRPVDLTLHSDVRAVERAEFDYQVAEKMSFIEQYKMERERQQKL 486

Query: 486 AEEEELRRLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSC--LPW 535
           AEEEE+RRLRKELVPKAQPMPYFDRPFIPRRS+K PT PR+PKFHIPQ KK + C    W
Sbjct: 487 AEEEEIRRLRKELVPKAQPMPYFDRPFIPRRSSKHPTAPRDPKFHIPQHKKIRCCSSSSW 487

BLAST of Carg02226 vs. TAIR 10
Match: AT3G01015.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 399.4 bits (1025), Expect = 4.5e-111
Identity = 261/521 (50.10%), Postives = 331/521 (63.53%), Query Frame = 0

Query: 26  QEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAKTAQKSAAK---NPSAVLNSPKYKIR 85
           QEVS  SEN+NPN          +  S+P  KS+K++ +   K   NP   + SP+ +IR
Sbjct: 26  QEVSRFSENSNPNF---------VSHSTPLEKSSKSSAQKNPKWKPNPVPAVFSPRNRIR 85

Query: 86  ERKFVVAKKNSKNEKSGKCKTDCKCEVNTRSDAKKCLCLAYETLRASQEEFFKNQSGGIG 145
           ER+FVV KKNS+ EK+     DCKC   T S+ KKC+C+AYETLRASQEEFF N+   + 
Sbjct: 86  ERRFVVVKKNSRKEKNDSASVDCKCGAKTISNMKKCVCIAYETLRASQEEFFNNRRESVS 145

Query: 146 EIDETEQLDGSIRAEIDLEEEMERKLMIQDLQNEDRAEAEAFDVNGESESPEQVSSSVIK 205
           EI E+ Q         +LE+  E+                      E    ++   S++K
Sbjct: 146 EIGESSQ---------NLEDGNEQV---------------------EFGDSDETRVSLMK 205

Query: 206 RRRERLLETARNSVPETGSGKVKHLVMAFEKLRTIRVSKDSDELSENQEKAMEEKNKVIK 265
           RRRE++LE AR S+PE   GKV HLV AFEKL    +SK + +  E+Q K      + +K
Sbjct: 206 RRREKVLEEARMSIPE--FGKVMHLVKAFEKLTCFPLSKVTSKEEEDQIK------QPLK 265

Query: 266 WALPGLPPPKVSEMQGS----SSSLCPSE-LFFTSENRALD-PAASVSSSWDSSRGSISS 325
           W LPG+  PK SE +      SSS  PS  L  T+ N  L+ P ASVSSSWD+   S+SS
Sbjct: 266 WELPGMSQPKCSESETDQFTWSSSFYPSSGLILTATNLGLEQPHASVSSSWDN---SVSS 325

Query: 326 RTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQKPFKLRTEQRGKEKGEEFLKRVQET 385
             S+GGRR RRNS ES  ++G  R  K+Q+KVT  KPFKLRTE+RG+ K EEF K++ E 
Sbjct: 326 LNSNGGRRGRRNSFESSASMGSRRSTKKQIKVTSLKPFKLRTEERGRMKEEEFAKKLHEM 385

Query: 386 MMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILRSHVGWRCLVKPPVKENTRPVDLVLH 445
            +E+ ++RIPIAQGLPWTTDEPE                   LVKP VK+ T PVDL LH
Sbjct: 386 TLEKAKKRIPIAQGLPWTTDEPEN------------------LVKPHVKDITIPVDLKLH 445

Query: 446 SDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRLRKELVPKAQPMPYFDR 505
           SD+RAVERAEFD QV EK++L+EQYK +RE+QQK+AEEEE+RRLRKELVPKAQPMPYFDR
Sbjct: 446 SDIRAVERAEFDYQVTEKINLVEQYKTERERQQKLAEEEEIRRLRKELVPKAQPMPYFDR 478

Query: 506 PFIPRRSAKLPTIPREPKFHIPQQKKSKSC--LPWNDVGSH 536
           PFIP+RS K PT+PR+PKF+IPQ KK + C    W+D GS+
Sbjct: 506 PFIPKRSNKHPTVPRDPKFNIPQHKKIRCCSTSSWSDTGSY 478

BLAST of Carg02226 vs. TAIR 10
Match: AT5G15510.2 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 383.6 bits (984), Expect = 2.5e-106
Identity = 269/511 (52.64%), Postives = 324/511 (63.41%), Query Frame = 0

Query: 6   KNGGTMTPVKDSTGSGLKMIQEVSGNSENTNPNVNDSISSVPKLWKSSPSMKSAKTAQKS 65
           KNG   TPVKD   S  +  Q++S +SEN NPN++ +  S       SP MKSAK   KS
Sbjct: 7   KNGKAGTPVKDR--SKFQSNQKISRSSENANPNISQASPS------KSPLMKSAK---KS 66

Query: 66  AAKNPS------AVLNSPKYKIRERKF-VVAKKNSKNEK-------SGKCKTDCKCEVNT 125
           A KNPS        + SP+ +IRERKF VVAKKNS+  K       S   + DCKC    
Sbjct: 67  AQKNPSPSPKPTQAVFSPRNRIRERKFVVVAKKNSRKGKKEPAITESKAAEIDCKCGERK 126

Query: 126 RSDAKKCLCLAYETLRASQEEFFKNQSGGIGEIDETEQLDGSIRAEIDLEEEMERKLMIQ 185
           + +  KC+C+AYETLRASQEEFFKN+                    I+ EE+ E      
Sbjct: 127 KGN-MKCVCVAYETLRASQEEFFKNR--------------------IESEEDKEALEECC 186

Query: 186 DLQNEDRAEAEAFDVNGESESPEQVSSSVIKRRRERLLETARNSVPETGSGKVKHLVMAF 245
           +L N D  E +      ES  PE+   S +KR R +++E AR SVP   SGKV +LV AF
Sbjct: 187 NLGNGDELEGK------ESGEPEKTGVSTMKRSRAKVVEEARQSVPV--SGKVMNLVEAF 246

Query: 246 EKLRTIRVSKDSDELSENQEKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLCPSELFFT 305
           EKL     SK ++++ ENQ +  E+  K +K  L  L   K  E    SSS CPSE+  T
Sbjct: 247 EKLTCFSNSKTANKIEENQTE--EDTKKPVK--LEFLEGEK--EKNPWSSSFCPSEMVLT 306

Query: 306 SENRALDPAASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVTRQ 365
           ++N  LDP AS+SSSWDS+RGS+ S +S+ GRRSRRNS ES  T+G  R KK+Q+KVT  
Sbjct: 307 AKNLGLDPNASISSSWDSTRGSVLSGSSNAGRRSRRNSMESSTTMGSRRSKKKQVKVTSL 366

Query: 366 KPFKLRTEQRGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTDEPEQGRWPLGGWILR 425
           KPFKLRTEQRGK K EE  K++ E  ME+E+ RIPIAQGLPWTTDEPE            
Sbjct: 367 KPFKLRTEQRGKVKEEELAKKIHEITMEEEKMRIPIAQGLPWTTDEPE------------ 426

Query: 426 SHVGWRCLVKPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKM 485
                 CLVKP  K+ TRPVDL LHSDVRAVERAEFD QVAEK+S IEQYKM+RE+QQK+
Sbjct: 427 ------CLVKPHWKDITRPVDLTLHSDVRAVERAEFDYQVAEKMSFIEQYKMERERQQKL 453

Query: 486 AEEEELRRLRKELVPKAQPMPYFDRPFIPRR 503
           AEEEE+RRLRKELVPKAQPMPYFDRPFIPRR
Sbjct: 487 AEEEEIRRLRKELVPKAQPMPYFDRPFIPRR 453

BLAST of Carg02226 vs. TAIR 10
Match: AT5G37478.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 100.9 bits (250), Expect = 3.3e-21
Identity = 56/122 (45.90%), Postives = 79/122 (64.75%), Query Frame = 0

Query: 419 LVKPPVKENTRPVDLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELR 478
           + K P KEN +P++  LHS  RAV+RA F+  VA    + +  K   E+ QKM EEEE+R
Sbjct: 56  ITKAPAKENKKPLEFKLHSGERAVKRAMFNYSVATNYYIQKLQKKQEERLQKMIEEEEIR 115

Query: 479 RLRKELVPKAQPMPYFDRPFIPRRSAKLPTIPREPKFHIPQQKKSKSCLPWNDVGSHHHY 538
            LRKE+VPKAQ MP+FDRPF+P+RS++  T+P+EP F       + +C  W  V ++ HY
Sbjct: 116 MLRKEMVPKAQLMPFFDRPFLPQRSSRPLTMPKEPSF----GNVNSTC--WTCVFNNQHY 171

Query: 539 TF 541
            +
Sbjct: 176 LY 171

BLAST of Carg02226 vs. TAIR 10
Match: AT1G03780.2 (targeting protein for XKLP2 )

HSP 1 Score: 73.9 bits (180), Expect = 4.3e-13
Identity = 86/339 (25.37%), Postives = 152/339 (44.84%), Query Frame = 0

Query: 239 VSKDSDELSENQ-EKAMEEKNKVIKWALPGLPPPKVSEMQGSSSSLC--------PSELF 298
           ++K + EL + + EKA + K K +          K+ E +G     C        P E  
Sbjct: 435 IAKTTAELEQEELEKAPKFKAKPLN--------KKIFESKGEMGIFCNTKKHITIPQEFH 494

Query: 299 FTSENRALDPAASVSSSWDSSRGSISSRTSHGGRRSRRNSTESDGTLGGSRWKKRQLKVT 358
           F ++ R   P  SV   +D  + S++S + H                     +K   + T
Sbjct: 495 FATDERISRP-ESVLDIFD--KLSLNSESCH---------------------EKPLPRNT 554

Query: 359 RQKPFKLRTEQRGKEKGEEFLKRVQETMMEQERQRIPIAQGLPWTTD--------EPEQG 418
              PF L+TE+RG EK ++F   +    +   + R+P A   P+TTD        EP+Q 
Sbjct: 555 APNPFNLKTEERGAEKEKKFYMELMYKKLGDVKARVPKANPYPYTTDYPVVPPKPEPKQC 614

Query: 419 RWP----LGGWI---------------LRSHVGWRCLVK--PPVKENTRPV--------- 478
             P    L   +               + +    + L K  P +KE+  PV         
Sbjct: 615 TQPEPFQLESLVRHEEEMRREREERRRMETEEAQKRLFKAQPVIKEDPIPVPEKVRMPLT 674

Query: 479 ---DLVLHSDVRAVERAEFDNQVAEKLSLIEQYKMDREKQQKMAEEEELRRLRKELVPKA 528
              +  LH + RAVERA+FD+++ EK +  ++Y+ + E  + + EE  L+++RK +VP A
Sbjct: 675 EIQEFNLHVEHRAVERADFDHKIKEKENQYKRYREESEAAKMVEEERALKQMRKTMVPHA 734