Carg02219 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg02219 vs. NCBI nr
Match: KAG7016107.1 (putative methylesterase 11, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 752.7 bits (1942), Expect = 1.6e-213
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL
Sbjct: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60

Query: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120
           FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI
Sbjct: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180

Query: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240
           TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ
Sbjct: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240

Query: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300
           AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP
Sbjct: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300

Query: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360
           ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP
Sbjct: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360

Query: 361 QALNKLLVDISKIKTQS 378
           QALNKLLVDISKIKTQS
Sbjct: 361 QALNKLLVDISKIKTQS 377

BLAST of Carg02219 vs. NCBI nr
Match: KAG6578546.1 (putative methylesterase 11, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 750.0 bits (1935), Expect = 1.0e-212
Identity = 375/377 (99.47%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDA+LTQQQILAAAIL
Sbjct: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDASLTQQQILAAAIL 60

Query: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120
           FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI
Sbjct: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180

Query: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240
           TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ
Sbjct: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240

Query: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300
           AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP
Sbjct: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300

Query: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360
           ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP
Sbjct: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360

Query: 361 QALNKLLVDISKIKTQS 378
           QALNKLLVDISKIKT+S
Sbjct: 361 QALNKLLVDISKIKTRS 377

BLAST of Carg02219 vs. NCBI nr
Match: XP_022938988.1 (putative methylesterase 11, chloroplastic [Cucurbita moschata])

HSP 1 Score: 744.6 bits (1921), Expect = 4.2e-211
Identity = 373/377 (98.94%), Postives = 375/377 (99.47%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGNFCGSLAP+PTKKRPTKRP NPPSSASSSKIWTRGRSSRKE PDAALTQQQILAAAIL
Sbjct: 1   MGNFCGSLAPKPTKKRPTKRPPNPPSSASSSKIWTRGRSSRKETPDAALTQQQILAAAIL 60

Query: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120
           FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI
Sbjct: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180

Query: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240
           TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ
Sbjct: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240

Query: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300
           AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP
Sbjct: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300

Query: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360
           ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP
Sbjct: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360

Query: 361 QALNKLLVDISKIKTQS 378
           QALNKLLVDISKIKT+S
Sbjct: 361 QALNKLLVDISKIKTRS 377

BLAST of Carg02219 vs. NCBI nr
Match: XP_023549629.1 (putative methylesterase 11, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 744.6 bits (1921), Expect = 4.2e-211
Identity = 372/377 (98.67%), Postives = 375/377 (99.47%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGNFCGSLAP+PTKKRPTKRPLNPPSSA+SSKIWTRGRSSRKEKPDAALT QQILAAAIL
Sbjct: 1   MGNFCGSLAPKPTKKRPTKRPLNPPSSANSSKIWTRGRSSRKEKPDAALTHQQILAAAIL 60

Query: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120
           FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI
Sbjct: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180

Query: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240
           TDFLEKLADGEKVILVGHDFGGACIAYAMELFP RIAKAIFIAAAMLSNGQSTLDMFSHQ
Sbjct: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPFRIAKAIFIAAAMLSNGQSTLDMFSHQ 240

Query: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300
           +GADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP
Sbjct: 241 SGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300

Query: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360
           ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP
Sbjct: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360

Query: 361 QALNKLLVDISKIKTQS 378
           QALNKLLVDISKIKTQS
Sbjct: 361 QALNKLLVDISKIKTQS 377

BLAST of Carg02219 vs. NCBI nr
Match: XP_022993630.1 (putative methylesterase 11, chloroplastic [Cucurbita maxima])

HSP 1 Score: 734.6 bits (1895), Expect = 4.4e-208
Identity = 367/377 (97.35%), Postives = 373/377 (98.94%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGNFCGSLAP+PTKKRPTKRPLNPPSSA+SSKIWTRGRSSRKEK +AALTQQQILAAAIL
Sbjct: 1   MGNFCGSLAPKPTKKRPTKRPLNPPSSANSSKIWTRGRSSRKEKHNAALTQQQILAAAIL 60

Query: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120
           FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI
Sbjct: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180

Query: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240
           TDFLEKLA+GEKVILVGHDFGGACIAYAMELFPSRIAKA+FIAAAMLSNGQSTLDMFSHQ
Sbjct: 181 TDFLEKLAEGEKVILVGHDFGGACIAYAMELFPSRIAKAVFIAAAMLSNGQSTLDMFSHQ 240

Query: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300
            GADDLMQQAQTFSYANGNDHPPTAINFEKSLL DIFF KSPAKDVALASVSMRPIPFAP
Sbjct: 241 VGADDLMQQAQTFSYANGNDHPPTAINFEKSLLWDIFFHKSPAKDVALASVSMRPIPFAP 300

Query: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360
           ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQ+VFRLKGADHSPFFSKP
Sbjct: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQKVFRLKGADHSPFFSKP 360

Query: 361 QALNKLLVDISKIKTQS 378
           QALNKLLVDISKIKTQS
Sbjct: 361 QALNKLLVDISKIKTQS 377

BLAST of Carg02219 vs. ExPASy Swiss-Prot
Match: Q9FW03 (Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES11 PE=1 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 1.3e-133
Identity = 248/388 (63.92%), Postives = 310/388 (79.90%), Query Frame = 0

Query: 1   MGNFCGSLA-PQPTKKRP--TKRPLNPPSSASSSKI----W---TRGRSSRKEK--PDAA 60
           MGN C     P+P KKR   TKR  +  +S+S S +    W    R  SSR++    DA 
Sbjct: 1   MGNLCSLFTPPKPVKKRKPITKRQSSIGASSSGSGLNSNRWNNRVRSSSSRRDNKFEDAL 60

Query: 61  LTQQQILAAAILFQQHHGRD---PFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPML 120
           + +  + AAA+LF+Q +G     PFDR++S RY  S SK  N LPRSSS+R+RS TDP+L
Sbjct: 61  IQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSKK-NQLPRSSSSRSRSSTDPLL 120

Query: 121 QPHQLVAQNVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSF 180
           QPHQ + Q +KL+D+ETNHFVLVHGG FGAWCWYKTIALLEE G+K TAIDL G G++S 
Sbjct: 121 QPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSI 180

Query: 181 DPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAA 240
           + N I SLSQYV+PLTD LEKL  GEKVILVGHDFGGACI+YAMELFPS+I+KA+F+AAA
Sbjct: 181 NINGIASLSQYVKPLTDILEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAA 240

Query: 241 MLSNGQSTLDMFSHQAGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKD 300
           ML+NGQSTLDMFS +AG +DLM++AQ F Y NGN++PPTAI+ +KSLL+D+ F +SP+KD
Sbjct: 241 MLTNGQSTLDMFSLKAGQNDLMRKAQIFIYTNGNENPPTAIDLDKSLLKDLLFNQSPSKD 300

Query: 301 VALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQV 360
           VALASVSMR IPFAP+LEKL LS++ YGS+ R+YIET EDNAIP+TLQE+M+N SPP++V
Sbjct: 301 VALASVSMRSIPFAPVLEKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKV 360

Query: 361 FRLKGADHSPFFSKPQALNKLLVDISKI 374
           +RLKGADH+PFFSKPQAL+KLL++I++I
Sbjct: 361 YRLKGADHAPFFSKPQALHKLLLEIARI 387

BLAST of Carg02219 vs. ExPASy Swiss-Prot
Match: F4IE65 (Putative methylesterase 13, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES13 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.4e-108
Identity = 213/403 (52.85%), Postives = 281/403 (69.73%), Query Frame = 0

Query: 8   LAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEK--------------PDAALTQQQ 67
           L+  P+ +R T  P    S +SS+    +G    K K               D+ + Q+Q
Sbjct: 36  LSLMPSFRRRTLLPSLSCSGSSSTSSSKKGGIKAKTKKIRERHHHHHQDHEKDSHIIQEQ 95

Query: 68  ILAAA-ILFQQHHGRD------PFDRTSSLRYPKSGSKNTNALP---------------R 127
            LAA  +LF Q            F R++S+ YP +    T++ P               R
Sbjct: 96  TLAATNLLFNQTPRNSNSVVPPSFRRSTSVVYPSAQPSGTSSGPVSAVQTPKKSSAGFVR 155

Query: 128 SSSTRARSLTDPMLQPHQLVAQNV-KLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGY 187
           SSS+R RS TDPM++P+QLV + + K+E  ET  FVLVHGGGFGAWCWYKTI LLE+ G+
Sbjct: 156 SSSSRQRSSTDPMIKPNQLVDKELNKVEGSETKRFVLVHGGGFGAWCWYKTITLLEKHGF 215

Query: 188 KATAIDLTGSGVHSFDPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACIAYAME 247
           +  A++LTGSGV S D N ITSL+ Y +PL  F E L   EKVILVGHDFGGAC++YAME
Sbjct: 216 QVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFFESLKPTEKVILVGHDFGGACMSYAME 275

Query: 248 LFPSRIAKAIFIAAAMLSNGQSTLDMFSHQAGADDLMQQAQTFSYANGNDHPPTAINFEK 307
           +FP++IAKA+FI+AAML+NGQSTLD+F+ Q G++DLMQQAQ F YANG  +PPTA++F++
Sbjct: 276 MFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSNDLMQQAQIFLYANGKKNPPTAVDFDR 335

Query: 308 SLLRDIFFQKSPAKDVALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPEDNAIPI 367
           SLLRD  F +SP KD+ALASVS+RPIPFAP+ EK+ +SE  YGSI RFYI+T ED A+P+
Sbjct: 336 SLLRDFLFNQSPPKDLALASVSIRPIPFAPVSEKVHVSEKNYGSIRRFYIKTMEDYAVPV 395

Query: 368 TLQESMMNKSPPQQVFRLKGADHSPFFSKPQALNKLLVDISKI 374
            LQE+M+  +PP+QVF+LKG+DH+PFFS+PQ+LNK+LV+IS+I
Sbjct: 396 LLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSLNKILVEISQI 438

BLAST of Carg02219 vs. ExPASy Swiss-Prot
Match: F4I0K9 (Putative methylesterase 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES15 PE=2 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 9.2e-108
Identity = 211/400 (52.75%), Postives = 281/400 (70.25%), Query Frame = 0

Query: 12  PTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEK---------------PDAALTQQQILA 71
           P+ +R T  P    S +S+S    +G    K+K                D+ +  Q + A
Sbjct: 39  PSFRRRTLLPSLSCSGSSTSSTSKKGGIKTKKKIRERHHQEQHHHDHEKDSLIQDQTLAA 98

Query: 72  AAILFQQ----HHGRDPFDRTSSLRYPK-------------SGS----KNTNALPRSSST 131
             ILF Q     +   PF R++S+ Y +             SG+    K+T    RSSS 
Sbjct: 99  TNILFSQTPRNSNSAPPFRRSTSVVYTQPPTAAVAASVGSVSGALTPKKSTYGYVRSSSN 158

Query: 132 RARSLTDPMLQPHQLVAQNVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAI 191
           R RS TDP+L+P+QL+ + +K+E  ET  FVLVHGGGFGAWCWYKTI LLE+ G++  A+
Sbjct: 159 RQRSSTDPVLKPNQLLDKELKVEGAETKRFVLVHGGGFGAWCWYKTITLLEKHGFQVDAV 218

Query: 192 DLTGSGVHSFDPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACIAYAMELFPSR 251
           DLTGSGV SFD N ITSL+QYV+PL  F + L   EKVILVGHDFGGAC++YAME++PS+
Sbjct: 219 DLTGSGVSSFDTNNITSLAQYVKPLLHFFDTLKPTEKVILVGHDFGGACMSYAMEMYPSK 278

Query: 252 IAKAIFIAAAMLSNGQSTLDMFSHQAGAD-DLMQQAQTFSYANGNDHPPTAINFEKSLLR 311
           IAKAIFI+AAML+N QSTLD+F+ Q  ++ DLM+Q   F YANG  +PPTA++F++SLLR
Sbjct: 279 IAKAIFISAAMLANAQSTLDLFNQQPDSNYDLMEQVHLFLYANGKKNPPTAVDFDRSLLR 338

Query: 312 DIFFQKSPAKDVALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPEDN-AIPITLQ 371
           D FF +SP KDVALASVSMRPIPFAP++EKL +SE  YGSI RFYI+T ED+ A+P++LQ
Sbjct: 339 DFFFNQSPPKDVALASVSMRPIPFAPVVEKLHVSEKNYGSIRRFYIKTMEDDYAVPVSLQ 398

Query: 372 ESMMNKSPPQQVFRLKGADHSPFFSKPQALNKLLVDISKI 374
           ++M+  +PP+QVF LKG+DH+PFFS+PQ+LN++LV+IS++
Sbjct: 399 DAMIKSNPPEQVFHLKGSDHAPFFSRPQSLNRILVEISQL 438

BLAST of Carg02219 vs. ExPASy Swiss-Prot
Match: Q9FVW3 (Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES14 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.4e-76
Identity = 151/344 (43.90%), Postives = 217/344 (63.08%), Query Frame = 0

Query: 31  SKIWTRGRSSRKEKPDAALTQQQILAAAILFQQHHGRDPFDRTSSLRYPKSGSKNTNALP 90
           SK     RS RK   D  +  ++ L+ AI   Q   R  FD + S R             
Sbjct: 21  SKSKRMNRSQRKLLADEEMLHRRALSMAIHQAQLSQR--FDGSMSRRV------------ 80

Query: 91  RSSSTRARSLTDPMLQPHQLVAQNVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGY 150
            S+STR R+L+DP     Q+   +   E +    FVLVHG GFGAWCWYK +A LEE G 
Sbjct: 81  GSTSTRKRTLSDPFSNGKQVPDFS---ESLIVKKFVLVHGEGFGAWCWYKMVASLEESGL 140

Query: 151 KATAIDLTGSGVHSFDPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACIAYAME 210
               +DLTG G +  D N +++L +Y +PL D LE L + EKVILVGH  GGA I+YA+E
Sbjct: 141 SPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALE 200

Query: 211 LFPSRIAKAIFIAAAMLSNGQSTLDMFSHQAG-ADDLMQQAQTFSYANGNDHPPTAINFE 270
            FP +I+KAIF+ A M+S+GQ   D+FS + G A+  M+++Q   Y NG D PPT   FE
Sbjct: 201 RFPEKISKAIFVCATMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFE 260

Query: 271 KSLLRDIFFQKSPAKDVALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPEDNAIP 330
           K  ++ ++F +SP KD+ALA +SMRP+P  P++EK+ L+  +YG   RFY++T +D A+ 
Sbjct: 261 KPHMKGLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDRALS 320

Query: 331 ITLQESMMNKSPPQQVFRLKGADHSPFFSKPQALNKLLVDISKI 374
             +QE ++ ++ P+ VF++KG+DH PFFSKPQ+L+K+L++I++I
Sbjct: 321 PDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIAQI 347

BLAST of Carg02219 vs. ExPASy Swiss-Prot
Match: Q940H7 (Putative methylesterase 12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES12 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 7.3e-73
Identity = 146/349 (41.83%), Postives = 216/349 (61.89%), Query Frame = 0

Query: 26  SSASSSKIWTRGRSSRKEKPDAALTQQQILAAAILFQQHHGRDPFDRTSSLRYPKSGSKN 85
           S    S+     RS RK   D     ++ L+ AI   Q      FD + S R        
Sbjct: 17  SGGDGSRSKRVNRSQRKLLADEENLHRRALSMAI--HQAQVSQRFDGSMSRRI------- 76

Query: 86  TNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDIETNHFVLVHGGGFGAWCWYKTIALL 145
                 S+S+R  +L+D      Q+      LE ++   FVLVHG GFGAWCWYKTIA L
Sbjct: 77  -----GSTSSRRGTLSDSFSNNKQVPE---FLESLKVKKFVLVHGEGFGAWCWYKTIASL 136

Query: 146 EEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACI 205
           EE G     +DL GSG +  D N +++L +Y +PL + ++ L   EKVILVGH  GGAC+
Sbjct: 137 EESGLSPVTVDLAGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEKVILVGHSTGGACV 196

Query: 206 AYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQAG-ADDLMQQAQTFSYANGNDHPPT 265
           +YA+E FP +I+KAIFI A M+++GQ   D+F+ + G A+  M+++Q   Y NG D+P T
Sbjct: 197 SYALERFPEKISKAIFICATMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPAT 256

Query: 266 AINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPE 325
              FEK  ++ ++F +SP KD+AL+ +SMRP+P  P++EKL LS  +YG   RFY++T +
Sbjct: 257 GFMFEKQHMKGLYFNQSPNKDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLD 316

Query: 326 DNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKPQALNKLLVDISKI 374
           D A+   +QE ++ ++ P+ VF++KG+DH PFFSKPQ+L+K+L++I++I
Sbjct: 317 DLALSPDVQEKLVRENSPEAVFKIKGSDHCPFFSKPQSLHKILLEIAQI 348

BLAST of Carg02219 vs. ExPASy TrEMBL
Match: A0A6J1FKF9 (putative methylesterase 11, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445033 PE=4 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.1e-211
Identity = 373/377 (98.94%), Postives = 375/377 (99.47%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGNFCGSLAP+PTKKRPTKRP NPPSSASSSKIWTRGRSSRKE PDAALTQQQILAAAIL
Sbjct: 1   MGNFCGSLAPKPTKKRPTKRPPNPPSSASSSKIWTRGRSSRKETPDAALTQQQILAAAIL 60

Query: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120
           FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI
Sbjct: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180

Query: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240
           TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ
Sbjct: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240

Query: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300
           AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP
Sbjct: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300

Query: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360
           ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP
Sbjct: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360

Query: 361 QALNKLLVDISKIKTQS 378
           QALNKLLVDISKIKT+S
Sbjct: 361 QALNKLLVDISKIKTRS 377

BLAST of Carg02219 vs. ExPASy TrEMBL
Match: A0A6J1JZ24 (putative methylesterase 11, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111489562 PE=4 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.1e-208
Identity = 367/377 (97.35%), Postives = 373/377 (98.94%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGNFCGSLAP+PTKKRPTKRPLNPPSSA+SSKIWTRGRSSRKEK +AALTQQQILAAAIL
Sbjct: 1   MGNFCGSLAPKPTKKRPTKRPLNPPSSANSSKIWTRGRSSRKEKHNAALTQQQILAAAIL 60

Query: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120
           FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI
Sbjct: 61  FQQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPL 180

Query: 181 TDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQ 240
           TDFLEKLA+GEKVILVGHDFGGACIAYAMELFPSRIAKA+FIAAAMLSNGQSTLDMFSHQ
Sbjct: 181 TDFLEKLAEGEKVILVGHDFGGACIAYAMELFPSRIAKAVFIAAAMLSNGQSTLDMFSHQ 240

Query: 241 AGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAP 300
            GADDLMQQAQTFSYANGNDHPPTAINFEKSLL DIFF KSPAKDVALASVSMRPIPFAP
Sbjct: 241 VGADDLMQQAQTFSYANGNDHPPTAINFEKSLLWDIFFHKSPAKDVALASVSMRPIPFAP 300

Query: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKP 360
           ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQ+VFRLKGADHSPFFSKP
Sbjct: 301 ILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQKVFRLKGADHSPFFSKP 360

Query: 361 QALNKLLVDISKIKTQS 378
           QALNKLLVDISKIKTQS
Sbjct: 361 QALNKLLVDISKIKTQS 377

BLAST of Carg02219 vs. ExPASy TrEMBL
Match: A0A1S3CBA3 (putative methylesterase 11, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498536 PE=4 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 3.1e-183
Identity = 326/380 (85.79%), Postives = 352/380 (92.63%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGN C  LAP+  KK+PTKR  +P    +SSK WTR RSSRK+KPDAALT++Q+LAAAIL
Sbjct: 1   MGNLCACLAPKSIKKKPTKRLPSPTLPNNSSKRWTRVRSSRKDKPDAALTREQVLAAAIL 60

Query: 61  F---QQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKL 120
           F   QQH+ RDPFDRT+SLRYPKSGSKN+N LPRSSS+RARSLTDP+LQPHQLV++NVK 
Sbjct: 61  FQQHQQHNARDPFDRTTSLRYPKSGSKNSNVLPRSSSSRARSLTDPLLQPHQLVSENVKP 120

Query: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYV 180
           EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPN ITSLSQYV
Sbjct: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNCITSLSQYV 180

Query: 181 QPLTDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMF 240
           QPLTDFLEKLA+GEKVILVGHDFGGACIAYAMELFP RIAKAIFIAAAML+NGQ+TLDMF
Sbjct: 181 QPLTDFLEKLAEGEKVILVGHDFGGACIAYAMELFPFRIAKAIFIAAAMLTNGQNTLDMF 240

Query: 241 SHQAGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIP 300
           S QAGADDLMQQAQTFSYANGN HPPTAIN EKSLLRD+FF +SPAKDVALASVSMRPIP
Sbjct: 241 SQQAGADDLMQQAQTFSYANGNSHPPTAINLEKSLLRDLFFNQSPAKDVALASVSMRPIP 300

Query: 301 FAPILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFF 360
           FAPILEKLCLS+ KYGS+ RFYIETPEDNAIPITLQ+SM+NKSPPQQVFRLKGADHSPFF
Sbjct: 301 FAPILEKLCLSDLKYGSVRRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFF 360

Query: 361 SKPQALNKLLVDISKIKTQS 378
           SKPQALNKLL++ISKIKT S
Sbjct: 361 SKPQALNKLLIEISKIKTPS 380

BLAST of Carg02219 vs. ExPASy TrEMBL
Match: A0A5D3BLC2 (Putative methylesterase 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002410 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.0e-183
Identity = 325/380 (85.53%), Postives = 353/380 (92.89%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGN C  +AP+  KK+PTKR  +P    +SSK WTR RSSRK+KPDAALT++Q+LAAAIL
Sbjct: 1   MGNLCACIAPKSIKKKPTKRLPSPTLPNNSSKRWTRVRSSRKDKPDAALTREQVLAAAIL 60

Query: 61  F---QQHHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKL 120
           F   QQH+ RDPFDRT+SLRYPKSGSKN+N LPRSSS+RARSLTDP+LQPHQLV++NVK 
Sbjct: 61  FQQHQQHNARDPFDRTTSLRYPKSGSKNSNVLPRSSSSRARSLTDPLLQPHQLVSENVKP 120

Query: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYV 180
           EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPN ITSLSQYV
Sbjct: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNCITSLSQYV 180

Query: 181 QPLTDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMF 240
           QPLTDFLEKLA+GEKVILVGHDFGGACIAYAMELFP RIAKAIFIAAAML+NGQ+TLDMF
Sbjct: 181 QPLTDFLEKLAEGEKVILVGHDFGGACIAYAMELFPFRIAKAIFIAAAMLTNGQNTLDMF 240

Query: 241 SHQAGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIP 300
           S QAGADDLMQQAQTFSYANGN+HPPTAIN EKSLLRD+FF +SPAKDVALASVSMRPIP
Sbjct: 241 SQQAGADDLMQQAQTFSYANGNNHPPTAINLEKSLLRDLFFNQSPAKDVALASVSMRPIP 300

Query: 301 FAPILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFF 360
           FAPILEKLCLS+ KYGS+ RFYIETPEDNAIPITLQ+SM+NKSPPQQVFRLKGADHSPFF
Sbjct: 301 FAPILEKLCLSDLKYGSVRRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFF 360

Query: 361 SKPQALNKLLVDISKIKTQS 378
           SKPQALNKLL++ISKIKT S
Sbjct: 361 SKPQALNKLLIEISKIKTPS 380

BLAST of Carg02219 vs. ExPASy TrEMBL
Match: A0A0A0KSG7 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G640000 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.9e-183
Identity = 326/380 (85.79%), Postives = 350/380 (92.11%), Query Frame = 0

Query: 1   MGNFCGSLAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEKPDAALTQQQILAAAIL 60
           MGNFC  LAP+  KK+P  R  NP    +SSK WTR RSSRK+KPDAALT+ Q+LAAAIL
Sbjct: 1   MGNFCACLAPKSIKKKPNHRLPNPTLPTNSSKRWTRVRSSRKDKPDAALTRDQVLAAAIL 60

Query: 61  FQQ---HHGRDPFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPMLQPHQLVAQNVKL 120
           FQQ   H+ RDPFDRT+SLRYPKSGSKN+NALPRSSS+RARSLTDP+LQPHQLV++NVK 
Sbjct: 61  FQQHQHHNARDPFDRTTSLRYPKSGSKNSNALPRSSSSRARSLTDPLLQPHQLVSENVKP 120

Query: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLSQYV 180
           EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPN ITSLSQYV
Sbjct: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNCITSLSQYV 180

Query: 181 QPLTDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAAMLSNGQSTLDMF 240
           QPLTDFLEKL +GEKVILVGHDFGGACIAYAMELFP RIAKAIFIAAAMLSNGQ+T DMF
Sbjct: 181 QPLTDFLEKLPEGEKVILVGHDFGGACIAYAMELFPFRIAKAIFIAAAMLSNGQNTSDMF 240

Query: 241 SHQAGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKDVALASVSMRPIP 300
           S QAGADDLMQQAQTFSYANGN+HPPTAIN EKSLLRD+FF +SPAKDVALASVSMRPIP
Sbjct: 241 SQQAGADDLMQQAQTFSYANGNNHPPTAINLEKSLLRDLFFNQSPAKDVALASVSMRPIP 300

Query: 301 FAPILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQVFRLKGADHSPFF 360
           FAPILEKLCLS+ KYGS+ RFYIETPEDNAIPITLQ+SM+NKSPPQQVFRLKGADHSPFF
Sbjct: 301 FAPILEKLCLSDLKYGSVRRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFF 360

Query: 361 SKPQALNKLLVDISKIKTQS 378
           SKPQALNKLL++ISKIKT S
Sbjct: 361 SKPQALNKLLIEISKIKTPS 380

BLAST of Carg02219 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 477.6 bits (1228), Expect = 9.0e-135
Identity = 248/388 (63.92%), Postives = 310/388 (79.90%), Query Frame = 0

Query: 1   MGNFCGSLA-PQPTKKRP--TKRPLNPPSSASSSKI----W---TRGRSSRKEK--PDAA 60
           MGN C     P+P KKR   TKR  +  +S+S S +    W    R  SSR++    DA 
Sbjct: 1   MGNLCSLFTPPKPVKKRKPITKRQSSIGASSSGSGLNSNRWNNRVRSSSSRRDNKFEDAL 60

Query: 61  LTQQQILAAAILFQQHHGRD---PFDRTSSLRYPKSGSKNTNALPRSSSTRARSLTDPML 120
           + +  + AAA+LF+Q +G     PFDR++S RY  S SK  N LPRSSS+R+RS TDP+L
Sbjct: 61  IQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSKK-NQLPRSSSSRSRSSTDPLL 120

Query: 121 QPHQLVAQNVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSF 180
           QPHQ + Q +KL+D+ETNHFVLVHGG FGAWCWYKTIALLEE G+K TAIDL G G++S 
Sbjct: 121 QPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSI 180

Query: 181 DPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACIAYAMELFPSRIAKAIFIAAA 240
           + N I SLSQYV+PLTD LEKL  GEKVILVGHDFGGACI+YAMELFPS+I+KA+F+AAA
Sbjct: 181 NINGIASLSQYVKPLTDILEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAA 240

Query: 241 MLSNGQSTLDMFSHQAGADDLMQQAQTFSYANGNDHPPTAINFEKSLLRDIFFQKSPAKD 300
           ML+NGQSTLDMFS +AG +DLM++AQ F Y NGN++PPTAI+ +KSLL+D+ F +SP+KD
Sbjct: 241 MLTNGQSTLDMFSLKAGQNDLMRKAQIFIYTNGNENPPTAIDLDKSLLKDLLFNQSPSKD 300

Query: 301 VALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPEDNAIPITLQESMMNKSPPQQV 360
           VALASVSMR IPFAP+LEKL LS++ YGS+ R+YIET EDNAIP+TLQE+M+N SPP++V
Sbjct: 301 VALASVSMRSIPFAPVLEKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKV 360

Query: 361 FRLKGADHSPFFSKPQALNKLLVDISKI 374
           +RLKGADH+PFFSKPQAL+KLL++I++I
Sbjct: 361 YRLKGADHAPFFSKPQALHKLLLEIARI 387

BLAST of Carg02219 vs. TAIR 10
Match: AT1G26360.1 (methyl esterase 13 )

HSP 1 Score: 393.7 bits (1010), Expect = 1.7e-109
Identity = 213/403 (52.85%), Postives = 281/403 (69.73%), Query Frame = 0

Query: 8   LAPQPTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEK--------------PDAALTQQQ 67
           L+  P+ +R T  P    S +SS+    +G    K K               D+ + Q+Q
Sbjct: 36  LSLMPSFRRRTLLPSLSCSGSSSTSSSKKGGIKAKTKKIRERHHHHHQDHEKDSHIIQEQ 95

Query: 68  ILAAA-ILFQQHHGRD------PFDRTSSLRYPKSGSKNTNALP---------------R 127
            LAA  +LF Q            F R++S+ YP +    T++ P               R
Sbjct: 96  TLAATNLLFNQTPRNSNSVVPPSFRRSTSVVYPSAQPSGTSSGPVSAVQTPKKSSAGFVR 155

Query: 128 SSSTRARSLTDPMLQPHQLVAQNV-KLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGY 187
           SSS+R RS TDPM++P+QLV + + K+E  ET  FVLVHGGGFGAWCWYKTI LLE+ G+
Sbjct: 156 SSSSRQRSSTDPMIKPNQLVDKELNKVEGSETKRFVLVHGGGFGAWCWYKTITLLEKHGF 215

Query: 188 KATAIDLTGSGVHSFDPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACIAYAME 247
           +  A++LTGSGV S D N ITSL+ Y +PL  F E L   EKVILVGHDFGGAC++YAME
Sbjct: 216 QVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFFESLKPTEKVILVGHDFGGACMSYAME 275

Query: 248 LFPSRIAKAIFIAAAMLSNGQSTLDMFSHQAGADDLMQQAQTFSYANGNDHPPTAINFEK 307
           +FP++IAKA+FI+AAML+NGQSTLD+F+ Q G++DLMQQAQ F YANG  +PPTA++F++
Sbjct: 276 MFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSNDLMQQAQIFLYANGKKNPPTAVDFDR 335

Query: 308 SLLRDIFFQKSPAKDVALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPEDNAIPI 367
           SLLRD  F +SP KD+ALASVS+RPIPFAP+ EK+ +SE  YGSI RFYI+T ED A+P+
Sbjct: 336 SLLRDFLFNQSPPKDLALASVSIRPIPFAPVSEKVHVSEKNYGSIRRFYIKTMEDYAVPV 395

Query: 368 TLQESMMNKSPPQQVFRLKGADHSPFFSKPQALNKLLVDISKI 374
            LQE+M+  +PP+QVF+LKG+DH+PFFS+PQ+LNK+LV+IS+I
Sbjct: 396 LLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSLNKILVEISQI 438

BLAST of Carg02219 vs. TAIR 10
Match: AT1G69240.1 (methyl esterase 15 )

HSP 1 Score: 391.7 bits (1005), Expect = 6.5e-109
Identity = 211/400 (52.75%), Postives = 281/400 (70.25%), Query Frame = 0

Query: 12  PTKKRPTKRPLNPPSSASSSKIWTRGRSSRKEK---------------PDAALTQQQILA 71
           P+ +R T  P    S +S+S    +G    K+K                D+ +  Q + A
Sbjct: 39  PSFRRRTLLPSLSCSGSSTSSTSKKGGIKTKKKIRERHHQEQHHHDHEKDSLIQDQTLAA 98

Query: 72  AAILFQQ----HHGRDPFDRTSSLRYPK-------------SGS----KNTNALPRSSST 131
             ILF Q     +   PF R++S+ Y +             SG+    K+T    RSSS 
Sbjct: 99  TNILFSQTPRNSNSAPPFRRSTSVVYTQPPTAAVAASVGSVSGALTPKKSTYGYVRSSSN 158

Query: 132 RARSLTDPMLQPHQLVAQNVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAI 191
           R RS TDP+L+P+QL+ + +K+E  ET  FVLVHGGGFGAWCWYKTI LLE+ G++  A+
Sbjct: 159 RQRSSTDPVLKPNQLLDKELKVEGAETKRFVLVHGGGFGAWCWYKTITLLEKHGFQVDAV 218

Query: 192 DLTGSGVHSFDPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACIAYAMELFPSR 251
           DLTGSGV SFD N ITSL+QYV+PL  F + L   EKVILVGHDFGGAC++YAME++PS+
Sbjct: 219 DLTGSGVSSFDTNNITSLAQYVKPLLHFFDTLKPTEKVILVGHDFGGACMSYAMEMYPSK 278

Query: 252 IAKAIFIAAAMLSNGQSTLDMFSHQAGAD-DLMQQAQTFSYANGNDHPPTAINFEKSLLR 311
           IAKAIFI+AAML+N QSTLD+F+ Q  ++ DLM+Q   F YANG  +PPTA++F++SLLR
Sbjct: 279 IAKAIFISAAMLANAQSTLDLFNQQPDSNYDLMEQVHLFLYANGKKNPPTAVDFDRSLLR 338

Query: 312 DIFFQKSPAKDVALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPEDN-AIPITLQ 371
           D FF +SP KDVALASVSMRPIPFAP++EKL +SE  YGSI RFYI+T ED+ A+P++LQ
Sbjct: 339 DFFFNQSPPKDVALASVSMRPIPFAPVVEKLHVSEKNYGSIRRFYIKTMEDDYAVPVSLQ 398

Query: 372 ESMMNKSPPQQVFRLKGADHSPFFSKPQALNKLLVDISKI 374
           ++M+  +PP+QVF LKG+DH+PFFS+PQ+LN++LV+IS++
Sbjct: 399 DAMIKSNPPEQVFHLKGSDHAPFFSRPQSLNRILVEISQL 438

BLAST of Carg02219 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 286.2 bits (731), Expect = 3.9e-77
Identity = 151/344 (43.90%), Postives = 217/344 (63.08%), Query Frame = 0

Query: 31  SKIWTRGRSSRKEKPDAALTQQQILAAAILFQQHHGRDPFDRTSSLRYPKSGSKNTNALP 90
           SK     RS RK   D  +  ++ L+ AI   Q   R  FD + S R             
Sbjct: 21  SKSKRMNRSQRKLLADEEMLHRRALSMAIHQAQLSQR--FDGSMSRRV------------ 80

Query: 91  RSSSTRARSLTDPMLQPHQLVAQNVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGY 150
            S+STR R+L+DP     Q+   +   E +    FVLVHG GFGAWCWYK +A LEE G 
Sbjct: 81  GSTSTRKRTLSDPFSNGKQVPDFS---ESLIVKKFVLVHGEGFGAWCWYKMVASLEESGL 140

Query: 151 KATAIDLTGSGVHSFDPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACIAYAME 210
               +DLTG G +  D N +++L +Y +PL D LE L + EKVILVGH  GGA I+YA+E
Sbjct: 141 SPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALE 200

Query: 211 LFPSRIAKAIFIAAAMLSNGQSTLDMFSHQAG-ADDLMQQAQTFSYANGNDHPPTAINFE 270
            FP +I+KAIF+ A M+S+GQ   D+FS + G A+  M+++Q   Y NG D PPT   FE
Sbjct: 201 RFPEKISKAIFVCATMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFE 260

Query: 271 KSLLRDIFFQKSPAKDVALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPEDNAIP 330
           K  ++ ++F +SP KD+ALA +SMRP+P  P++EK+ L+  +YG   RFY++T +D A+ 
Sbjct: 261 KPHMKGLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDRALS 320

Query: 331 ITLQESMMNKSPPQQVFRLKGADHSPFFSKPQALNKLLVDISKI 374
             +QE ++ ++ P+ VF++KG+DH PFFSKPQ+L+K+L++I++I
Sbjct: 321 PDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIAQI 347

BLAST of Carg02219 vs. TAIR 10
Match: AT4G09900.1 (methyl esterase 12 )

HSP 1 Score: 275.8 bits (704), Expect = 5.2e-74
Identity = 146/349 (41.83%), Postives = 216/349 (61.89%), Query Frame = 0

Query: 26  SSASSSKIWTRGRSSRKEKPDAALTQQQILAAAILFQQHHGRDPFDRTSSLRYPKSGSKN 85
           S    S+     RS RK   D     ++ L+ AI   Q      FD + S R        
Sbjct: 17  SGGDGSRSKRVNRSQRKLLADEENLHRRALSMAI--HQAQVSQRFDGSMSRRI------- 76

Query: 86  TNALPRSSSTRARSLTDPMLQPHQLVAQNVKLEDIETNHFVLVHGGGFGAWCWYKTIALL 145
                 S+S+R  +L+D      Q+      LE ++   FVLVHG GFGAWCWYKTIA L
Sbjct: 77  -----GSTSSRRGTLSDSFSNNKQVPE---FLESLKVKKFVLVHGEGFGAWCWYKTIASL 136

Query: 146 EEGGYKATAIDLTGSGVHSFDPNRITSLSQYVQPLTDFLEKLADGEKVILVGHDFGGACI 205
           EE G     +DL GSG +  D N +++L +Y +PL + ++ L   EKVILVGH  GGAC+
Sbjct: 137 EESGLSPVTVDLAGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEKVILVGHSTGGACV 196

Query: 206 AYAMELFPSRIAKAIFIAAAMLSNGQSTLDMFSHQAG-ADDLMQQAQTFSYANGNDHPPT 265
           +YA+E FP +I+KAIFI A M+++GQ   D+F+ + G A+  M+++Q   Y NG D+P T
Sbjct: 197 SYALERFPEKISKAIFICATMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPAT 256

Query: 266 AINFEKSLLRDIFFQKSPAKDVALASVSMRPIPFAPILEKLCLSESKYGSIGRFYIETPE 325
              FEK  ++ ++F +SP KD+AL+ +SMRP+P  P++EKL LS  +YG   RFY++T +
Sbjct: 257 GFMFEKQHMKGLYFNQSPNKDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLD 316

Query: 326 DNAIPITLQESMMNKSPPQQVFRLKGADHSPFFSKPQALNKLLVDISKI 374
           D A+   +QE ++ ++ P+ VF++KG+DH PFFSKPQ+L+K+L++I++I
Sbjct: 317 DLALSPDVQEKLVRENSPEAVFKIKGSDHCPFFSKPQSLHKILLEIAQI 348