Carg02179 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02179
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerase II subunit 4
Location	Carg_Chr15: 1083872 .. 1087036 (-)
RNA-Seq Expression	Carg02179
Synteny	Carg02179

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATATTTTCCTCACAGTAATGGGCCTTCTTTGAGTAACGTCTGAGTTGGAAACGAAGAACCGCCCTCTCTGATTTTGCCGTCACGTATTGCCGCCGGATCTCGCCCCTAACCCTCTCTGTGTTTGTGGTGCGTCCTCTCTCAGGCCGTTCTCTATTTGAAATCTCATCAAGCTGATTATTAATGCATGCATTTTTATAATTCCAGACGTTGGTAGAATTTGGTATTCATCTCCATCTCCGATTTCGAAGATTACGAATCTGTTCTTCTTTGAAAGTTGGAAATTTTTGTTTTTCTGTTCTTTTTTTTTAATCGGTATGTTGGTGTTTAGGCATTTAGTTAGGGCTCAAATGCCCCTCTCTGTTCCCTAATGCTGCTGATTATTGTGATTAATGGAAAGTTGATTTGGGCTTCATTTAAATCTGAAATCTATCCGTCATTCACCTCGAAAAGATTCGAGATTAACCTCGAACAAGTTGGATGGAGCTTTTGCAATTTGAATTGAAGATTTTATGTTCCTAGGCTCGCGAAGTGTATTACTAACCTTAAAACTTTGTTTGATTGATGGGGAACGTTAATAGAATTGTGATCTATGTGAGTGTTTTCATGGGGAATGGGAAACGTTAATGACTTACATTACGAAAACAATTTCTATATGTTATAGACTAAATCCTTCAAAAGTAAATTGAAGAATCTTTAGTGCCATACCTTATTCAGAGTCTTCTTTCATAAGGTTATCAATTTTATATATCGTTAGTTGTTGACTCTGAATTTTCCGTTCTTTTACTATACTTCTGATGCCAGGCTATGTGGTAGGAAGTTTGCTGTGTGGAATGGAGATTTGATTTATTAGTCTAGTTTCGCAACGGGATCTTTTGTAATATCAATTTTGTAATTTGATCTTGCTCTAATCATTTCCAGATTTTGTAGGTAGTCCAAAACTTCAACTTCAGGCATGTCTGGAGAGGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGGTACCGATCTTTCTATGAATCTTTGATACTCTTTTAGTATTCAATGAGATTTTATGTCTCTCAAATGTCTTTCAACGATGTGTGTTTCATTCGTATATTTGAAATTTTGAATCTGATGTGCAATTCATGTATTTAATCTTTAAATTTGCTTCAAAACTGAGAAGCTTAATTTATTACGTTGTTCCCCATGCCTTATTATACGTTTCCTTCACATTCTTTGAACCTTCTAATAGCCAATGTGATTTTTTTTTTTTTAACAGTGCGTATGGCTTGGACTGAATATGTGGATTGAATGTAAACACACTGATTAATCCTGAAAGTAGTTTCATGGGTAGAATAGCATTGTAAAGAAATTGCTTTTGTTTTTTTGGTAAGAAACAGAGCGTTCATTGAGCAAAATAAAGTAATGTACGAGCACTTGCAAAGAAAAAAGGGCCTTCAAAAGGAGTCATCAATTATAAGCAAAGAAATCACGAGCTCTTATATGGTTCTAAGGTTTCTGAAATTTTAGATGAAGATATGTGATCTATGCAAAATCACATAAACATAACTATTATAAGCTTTTCCATGACAATGCAAGGTGCTTTAGACTAGCCAAATCTTTTTGGTTTTTATGGATAAGTAACCATTCGGAATTTAAGCTCTTGCGATCGAAATTGTTTTAGGGAGTACTCTTAAGCTTTTTCATTCCCTTGCATTTATTTCGCTCTTTGTTATAAGGTACATAAAAGGAAAAATATAGAATGTGATAATTTTCATCTACTTCTGTTGGTTGCCTGCAGAGTTCTTAAAAGCCAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTTGAACACAAGTATGAGCAGCTTCAACAAATGTCTGAGGATCCAACCAATCAAGTTTCTCAGTGAGTTTATGTTTATTCTCTATTGTTTCATAAGTTAATTTAGAGTTTCTCTTCCTTGCTGGAATAGCTGAAATATATAGCTAAATTATGATATTCATTCTAACTTTCCACACAATACGAGGGATTTTTAATTCTGGTCTTCTTCTGCAGAGTGTTTGAAAAGTCTCTGCAATATGTGAAACGGTTCAGCCGCTATAAGAACCCCGATGCTGTTAGACAAGTCCGAGAGTATCCTTTAAACTGTACATGTTAATATCTTACATTTTGTGGGATGGTGGGACGGATGGGTTGTGCATATCCACGATATGCTATAACTTACCCCATTTTGAACTAGGACTTTACAACTGCAATTCTCTTGCTAGACCAAGAACTCTCTGGTTAGCTGTTATTTAGGAAATAGTGAATAAATTAGCTAAAGTTCATTATATCTCAGCAGCTATACTATATTATAGGTTTCCTAGGTATCCATCTGAAATGATCCACTACTTTGATATTTCATTCATGACTTTTATCACGTAAGTTACAAACGAAAGTTTGGGTTCATCATTTAAAATCGCATCGAAGAGGTTAAATCTTAACTACTACCATATAGAATTCTAAGCAGGTATCAGTTGGCTGAGTTTGAGGTATGTGTACTGACTCACATGGTTTAATCAACTTGCTGATTATTATTCTATGGGAAGGTTTATCTCATCGCTCTTGAAATTTGCAGCTCTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTATTTCCATGGTTCCATCATTGAAGGTACCTTTTGCCACCCTTTTTAGGCCCTCATTAAATGGTTGTTTATTTTTTGGTATTTTGCCAATCTCAAATAGGTACCAACTTTTAGAAAGTTTACTGTTGGGTTTATCTTTCAACAACGAACATGCATCGACTTTTAAATGATCTCTAAAAAGTTTTAAAACTGAAGATGTAATTTTTTTAAATTTGTGAAAGATTTCATAGGTATTTCCAAAAAGTGAAAAAAAATTGTTTATAGATGGATCAGAGTCGCTTATAAATGCTTTTGTTGCAGACTAAAGGAAGGGCACATGACGATGAAGCAATCGAGAAGATGTTGAACGATCTTTCATTAATCAAAAAGTTCGAGTAGTTAGCTCTCTAAATCTGGGTTCAATGTGTTAATCCATTCCTTGGCATTTCTCACCTAAAACAATAGAGAATTGTACCCTTCTAATCAGAGTCTAATAACAAGTTTTTAGGCATTTACCTAAACGTTCAATTTTATTGC

mRNA sequence

ATATATTTTCCTCACAGTAATGGGCCTTCTTTGAGTAACGTCTGAGTTGGAAACGAAGAACCGCCCTCTCTGATTTTGCCGTCACGTATTGCCGCCGGATCTCGCCCCTAACCCTCTCTGTGTTTGTGGTAGTCCAAAACTTCAACTTCAGGCATGTCTGGAGAGGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCCAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTTGAACACAAGTATGAGCAGCTTCAACAAATGTCTGAGGATCCAACCAATCAAGTTTCTCAAGTGTTTGAAAAGTCTCTGCAATATGTGAAACGGTTCAGCCGCTATAAGAACCCCGATGCTGTTAGACAAGTCCGAGAAATTCTAAGCAGGTATCAGTTGGCTGAGTTTGAGCTCTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTATTTCCATGGTTCCATCATTGAAGACTAAAGGAAGGGCACATGACGATGAAGCAATCGAGAAGATGTTGAACGATCTTTCATTAATCAAAAAGTTCGAGTAGTTAGCTCTCTAAATCTGGGTTCAATGTGTTAATCCATTCCTTGGCATTTCTCACCTAAAACAATAGAGAATTGTACCCTTCTAATCAGAGTCTAATAACAAGTTTTTAGGCATTTACCTAAACGTTCAATTTTATTGC

Coding sequence (CDS)

ATGTCTGGAGAGGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCCAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTTGAACACAAGTATGAGCAGCTTCAACAAATGTCTGAGGATCCAACCAATCAAGTTTCTCAAGTGTTTGAAAAGTCTCTGCAATATGTGAAACGGTTCAGCCGCTATAAGAACCCCGATGCTGTTAGACAAGTCCGAGAAATTCTAAGCAGGTATCAGTTGGCTGAGTTTGAGCTCTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTATTTCCATGGTTCCATCATTGAAGACTAAAGGAAGGGCACATGACGATGAAGCAATCGAGAAGATGTTGAACGATCTTTCATTAATCAAAAAGTTCGAGTAG

Protein sequence

MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHDDEAIEKMLNDLSLIKKFE

Homology

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BLAST of Carg02179 vs. NCBI nr
Match: XP_022939401.1 (DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022993195.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_022993196.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_023549816.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >XP_023549818.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >KAG6578503.1 DNA-directed RNA polymerase II subunit 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016067.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 271.2 bits (692), Expect = 5.0e-69
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Carg02179 vs. NCBI nr
Match: XP_022133507.1 (DNA-directed RNA polymerase II subunit 4 [Momordica charantia])

HSP 1 Score: 267.7 bits (683), Expect = 5.6e-68
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Carg02179 vs. NCBI nr
Match: XP_038884885.1 (DNA-directed RNA polymerase II subunit 4 [Benincasa hispida])

HSP 1 Score: 267.3 bits (682), Expect = 7.3e-68
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

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BLAST of Carg02179 vs. NCBI nr
Match: XP_004138390.1 (DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_008456795.1 PREDICTED: DNA-directed RNA polymerase II subunit 4 [Cucumis melo] >XP_011656444.1 DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >KAA0051957.1 DNA-directed RNA polymerase II subunit 4 [Cucumis melo var. makuwa] >KGN45845.1 hypothetical protein Csa_005663 [Cucumis sativus] >TYK04594.1 DNA-directed RNA polymerase II subunit 4 [Cucumis melo var. makuwa])

HSP 1 Score: 266.9 bits (681), Expect = 9.5e-68
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]

BLAST of Carg02179 vs. NCBI nr
Match: XP_030924038.1 (DNA-directed RNA polymerase II subunit 4 [Quercus lobata] >KAB1210402.1 DNA-directed RNA polymerase II subunit 4 [Morella rubra] >KAF3942865.1 hypothetical protein CMV_030520 [Castanea mollissima])

HSP 1 Score: 265.8 bits (678), Expect = 2.1e-67
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAI+MVPS+KTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Carg02179 vs. ExPASy Swiss-Prot
Match: O48890 (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 2.6e-68
Identity = 129/138 (93.48%), Postives = 136/138 (98.55%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ+SEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA++MVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]

BLAST of Carg02179 vs. ExPASy Swiss-Prot
Match: O15514 (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 5.3e-21
Identity = 55/137 (40.15%), Postives = 93/137 (67.88%), Query Frame = 0


Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q +  S +   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNE--SAEDEQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ +++PSL  +GR  +D
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGR-FED 129

Query: 122 EAIEKMLNDLSLIKKFE 139
           E ++++L+D+   + F+
Sbjct: 130 EELQQILDDIQTKRSFQ 141

[Close]

BLAST of Carg02179 vs. ExPASy Swiss-Prot
Match: Q9D7M8 (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 5.3e-21
Identity = 55/137 (40.15%), Postives = 93/137 (67.88%), Query Frame = 0


Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q +  S +   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNE--SAEDEQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ +++PSL  +GR  +D
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGR-FED 129

Query: 122 EAIEKMLNDLSLIKKFE 139
           E ++++L+D+   + F+
Sbjct: 130 EELQQILDDIQTKRSFQ 141

[Close]

BLAST of Carg02179 vs. ExPASy Swiss-Prot
Match: Q54S04 (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 8.5e-19
Identity = 55/147 (37.41%), Postives = 88/147 (59.86%), Query Frame = 0


Query: 4   EEEENAAELKIGDEFLKAKCLMNCEVSLILEHK----------YEQ---LQQMSEDPTNQ 63
           +EEE+   LK   +   AK L+N EV+++LEH+          + Q   L  +  +  N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 64  VSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVP 123
           + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE+  L NLCPE  +EA S++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

Query: 124 SLKTKGRAHDDEAIEKMLNDLSLIKKF 138
           SLK      +D+ ++ +L++LS ++KF
Sbjct: 132 SLKKM----EDDELQAILDELSNLRKF 154

[Close]

BLAST of Carg02179 vs. ExPASy Swiss-Prot
Match: Q9VEA5 (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 86.7 bits (213), Expect = 2.3e-16
Identity = 48/126 (38.10%), Postives = 80/126 (63.49%), Query Frame = 0


Query: 6   EENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRF 65
           +E+AA+L+   EF  A+ L+  EV ++L+H+  + Q  S D   + S+VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSF 70

Query: 66  SRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHDDEAIE 125
            ++KN + +   R +L + +L +FEL  LGNLCPE  EEA +++PSL  +GR  +DE + 
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSL--EGR-FEDEELR 130

Query: 126 KMLNDL 132
           ++L+D+
Sbjct: 131 QILDDI 131

[Close]

BLAST of Carg02179 vs. ExPASy TrEMBL
Match: A0A6J1JVN3 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489290 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.4e-69
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Carg02179 vs. ExPASy TrEMBL
Match: A0A6J1FFS7 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=LOC111445330 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.4e-69
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Carg02179 vs. ExPASy TrEMBL
Match: A0A6J1BWW4 (DNA-directed RNA polymerase II subunit 4 OS=Momordica charantia OX=3673 GN=LOC111006069 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.7e-68
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Carg02179 vs. ExPASy TrEMBL
Match: A0A5D3C019 (DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G002310 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.6e-68
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]

BLAST of Carg02179 vs. ExPASy TrEMBL
Match: A0A0A0K7V5 (RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014650 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.6e-68
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]

BLAST of Carg02179 vs. TAIR 10
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 259.2 bits (661), Expect = 1.8e-69
Identity = 129/138 (93.48%), Postives = 136/138 (98.55%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ+SEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA++MVPSLKTKGRAHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]

BLAST of Carg02179 vs. TAIR 10
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 84.3 bits (207), Expect = 8.1e-17
Identity = 44/118 (37.29%), Postives = 76/118 (64.41%), Query Frame = 0


Query: 21  AKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREI 80
           A+C+M+CE   IL+    QL  +SEDP+ ++   ++++L YV+    Y NP +VR+V E 
Sbjct: 89  AECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEP 148

Query: 81  LSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHDDEAIEKMLNDLSLIKKFE 139
           L  Y +++ E+CV+ N   E+V+E ++ +PSLKTK +   ++ ++  L +LS +KK E
Sbjct: 149 LKTYGISDGEMCVIANASSESVDEVLAFIPSLKTK-KEVINQPLQDALEELSKLKKSE 205

The following BLAST results are available for this feature:

BLAST of Carg02179 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022939401.1	5.0e-69	100.00	DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402...	[more]
XP_022133507.1	5.6e-68	98.55	DNA-directed RNA polymerase II subunit 4 [Momordica charantia]	[more]
XP_038884885.1	7.3e-68	97.83	DNA-directed RNA polymerase II subunit 4 [Benincasa hispida]	[more]
XP_004138390.1	9.5e-68	97.10	DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_008456795.1 PREDI...	[more]
XP_030924038.1	2.1e-67	97.10	DNA-directed RNA polymerase II subunit 4 [Quercus lobata] >KAB1210402.1 DNA-dire...	[more]

BLAST of Carg02179 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48890	2.6e-68	93.48	DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB...	[more]
O15514	5.3e-21	40.15	DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE...	[more]
Q9D7M8	5.3e-21	40.15	DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d P...	[more]
Q54S04	8.5e-19	37.41	DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689...	[more]
Q9VEA5	2.3e-16	38.10	DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 G...	[more]

BLAST of Carg02179 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JVN3	2.4e-69	100.00	DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1FFS7	2.4e-69	100.00	DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1BWW4	2.7e-68	98.55	DNA-directed RNA polymerase II subunit 4 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A5D3C019	4.6e-68	97.10	DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A0A0K7V5	4.6e-68	97.10	RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014650 PE=4...	[more]

BLAST of Carg02179 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09920.1	1.8e-69	93.48	RNA polymerase II, Rpb4, core protein	[more]
AT4G15950.1	8.1e-17	37.29	RNA polymerase II, Rpb4, core protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006590	RNA polymerase Rpb4/RPC9, core	SMART	SM00657	rpol4neu2	coord: 16..138 e-value: 1.4E-58 score: 210.6
IPR038324	Rpb4/RPC9 superfamily	GENE3D	1.20.1250.40		coord: 2..138 e-value: 3.1E-51 score: 174.6
IPR005574	RNA polymerase subunit RPB4/RPC9	PFAM	PF03874	RNA_pol_Rpb4	coord: 23..132 e-value: 1.1E-23 score: 83.9
None	No IPR available	PANTHER	PTHR21297:SF3	DNA-DIRECTED RNA POLYMERASE II SUBUNIT 4-LIKE	coord: 1..138
None	No IPR available	PANTHER	PTHR21297	DNA-DIRECTED RNA POLYMERASE II	coord: 1..138
IPR010997	HRDC-like superfamily	SUPERFAMILY	47819	HRDC-like	coord: 3..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02179-RA	Carg02179-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031990	mRNA export from nucleus in response to heat stress
biological_process	GO:0000288	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process	GO:0045948	positive regulation of translational initiation
biological_process	GO:0034402	recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0006352	DNA-templated transcription, initiation
cellular_component	GO:0000932	P-body
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0030880	RNA polymerase complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0031369	translation initiation factor binding