Carg02168 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg02168 vs. NCBI nr
Match: KAG7016056.1 (Methylesterase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 718.8 bits (1854), Expect = 2.4e-203
Identity = 358/358 (100.00%), Postives = 358/358 (100.00%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180
           AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG
Sbjct: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180

Query: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240
           SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA
Sbjct: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 300
           VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD
Sbjct: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 300

Query: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN 359
           RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN
Sbjct: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN 358

BLAST of Carg02168 vs. NCBI nr
Match: KAG6578491.1 (Methylesterase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 534.6 bits (1376), Expect = 6.3e-148
Identity = 282/358 (78.77%), Postives = 282/358 (78.77%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180
           AATMLRFGFQTDQDIKN                                           
Sbjct: 121 AATMLRFGFQTDQDIKN------------------------------------------- 180

Query: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240
                                            GVPDLSEFGDAYDVGFGQGPQQPPTTA
Sbjct: 181 ---------------------------------GVPDLSEFGDAYDVGFGQGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 300
           VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD
Sbjct: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 282

Query: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN 359
           RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN
Sbjct: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN 282

BLAST of Carg02168 vs. NCBI nr
Match: XP_022938905.1 (methylesterase 17 [Cucurbita moschata] >XP_023551105.1 methylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528.5 bits (1360), Expect = 4.5e-146
Identity = 281/358 (78.49%), Postives = 281/358 (78.49%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180
           AATMLRFGFQTDQDIKN                                           
Sbjct: 121 AATMLRFGFQTDQDIKN------------------------------------------- 180

Query: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240
                                            GVPDLSEFGDAYDVGFGQGPQQPPTTA
Sbjct: 181 ---------------------------------GVPDLSEFGDAYDVGFGQGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 300
           VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD
Sbjct: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 281

Query: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN 359
           RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK AAAAAAASSTAACN
Sbjct: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK-AAAAAAASSTAACN 281

BLAST of Carg02168 vs. NCBI nr
Match: XP_022993026.1 (methylesterase 17 [Cucurbita maxima])

HSP 1 Score: 520.0 bits (1338), Expect = 1.6e-143
Identity = 276/358 (77.09%), Postives = 277/358 (77.37%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180
           AATMLRFGFQTDQDIKN                                           
Sbjct: 121 AATMLRFGFQTDQDIKN------------------------------------------- 180

Query: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240
                                            GVPDLSEFGD YDVGFG GPQQPPTTA
Sbjct: 181 ---------------------------------GVPDLSEFGDVYDVGFGLGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 300
           V+KRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELD VKRVYIRTTYD
Sbjct: 241 VIKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDTVKRVYIRTTYD 280

Query: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN 359
           RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK  AAAAAASSTAACN
Sbjct: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK--AAAAAASSTAACN 280

BLAST of Carg02168 vs. NCBI nr
Match: XP_038890470.1 (methylesterase 17 [Benincasa hispida])

HSP 1 Score: 461.1 bits (1185), Expect = 8.9e-126
Identity = 237/346 (68.50%), Postives = 254/346 (73.41%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV+VQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVDSVSVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           D SDPNSV+KFDDYNKPLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DRSDPNSVLKFDDYNKPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180
           AATMLRFGFQTD+D K+                                           
Sbjct: 121 AATMLRFGFQTDRDFKD------------------------------------------- 180

Query: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240
                                            GVPDLSEFGD YDV FG GPQQPPTTA
Sbjct: 181 ---------------------------------GVPDLSEFGDVYDVEFGLGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 300
           V+KRE QRKIIYHMSPHEDSTLA+MLLRPGPILAL+TA+F EE    D+VKRVYIRT YD
Sbjct: 241 VIKREFQRKIIYHMSPHEDSTLAAMLLRPGPILALKTAQFMEEQDGADKVKRVYIRTMYD 270

Query: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAA 347
           RVIKPEQQ+AMI+ W PEIVYEMDTDHSPFFS+PSLLFGLLVK+ +
Sbjct: 301 RVIKPEQQDAMIKGWPPEIVYEMDTDHSPFFSDPSLLFGLLVKSCS 270

BLAST of Carg02168 vs. ExPASy Swiss-Prot
Match: Q9SG92 (Methylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=MES17 PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 5.5e-86
Identity = 161/336 (47.92%), Postives = 211/336 (62.80%), Query Frame = 0

Query: 18  QKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKP 77
           +KPPHFVL+HG+S G WCWYKI+CLME SGF VTCIDLK +GID S  +S+  FD YN+P
Sbjct: 16  RKPPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQYNQP 75

Query: 78  LLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKN 137
           L+DF+SS PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+ML+ G QTD+D+K+
Sbjct: 76  LIDFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDEDMKD 135

Query: 138 VNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVWNIFRGFVRK 197
                                                                       
Sbjct: 136 ------------------------------------------------------------ 195

Query: 198 KVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPH 257
                           GVPDLSE GD Y++GFG GP+ PPT+A++K E +RK++YHMSP 
Sbjct: 196 ----------------GVPDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQ 255

Query: 258 EDSTLASMLLRPGPILALRTARFRE---ENGELDRVKRVYIRTTYDRVIKPEQQEAMIRE 317
           ++ +LA++++RP PILAL TA+  E   E G+ ++V RVYI+T  DRV+KPEQQ+AMIR 
Sbjct: 256 QECSLAALMMRPAPILALTTAKLEEEEKEKGQEEQVPRVYIKTLLDRVMKPEQQDAMIRR 275

Query: 318 WLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAA 351
           W P  VYE+++DHSPFFSNP +LFGLL+KAA +  +
Sbjct: 316 WPPSQVYELESDHSPFFSNPFVLFGLLIKAAVSVGS 275

BLAST of Carg02168 vs. ExPASy Swiss-Prot
Match: Q9LVL9 (Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-48
Identity = 118/327 (36.09%), Postives = 163/327 (49.85%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG   GGWCWYK+   + ++G K TCIDLKGAGI+ +DPN+V   DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNVNKF 141
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M++ G    + +KNV K 
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMK- 124

Query: 142 FFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVWNIFRGFVRKKVKI 201
                        IC                S ++     KI                  
Sbjct: 125 -------------IC----------------SGLIEEETEKI------------------ 184

Query: 202 RLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEDST 261
                                  +D  FG GPQ  PT+ ++K E  R   Y+ SP ED T
Sbjct: 185 -----------------------WDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPMEDYT 244

Query: 262 LASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVY 321
           LA+ LLRP P++A           E D++ RVY++T  D + +P  QE M+  W P   +
Sbjct: 245 LATTLLRPAPVMAFIGIMDIPGAPETDKIPRVYVKTGKDHLFEPVLQEVMLALWPPAHTF 260

Query: 322 EM-DTDHSPFFSNPSLLFGLLVKAAAA 348
            + D+DHS FFS P  L+  L++AA++
Sbjct: 305 LLPDSDHSAFFSQPQELYQFLLQAASS 260

BLAST of Carg02168 vs. ExPASy Swiss-Prot
Match: Q2V0W1 (Pheophorbidase OS=Raphanus sativus OX=3726 GN=PPD PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.9e-48
Identity = 116/326 (35.58%), Postives = 162/326 (49.69%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG S G WCWYK+  L+  +GFK T +DL GAGI+L+D N+V  FD YN+PL   
Sbjct: 13  HFVFVHGASHGAWCWYKLTTLLVAAGFKATSVDLTGAGINLTDSNTVFDFDHYNRPLFSL 72

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNVNKF 141
           +S LP + +I+LVGHS GG SVT+A  KF  KI + VY+AA M++ G             
Sbjct: 73  LSDLPSHHKIVLVGHSIGGGSVTEALCKFTDKISMVVYLAADMVQPG------------- 132

Query: 142 FFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVWNIFRGFVRKKVKI 201
                                     S ++DSIM                          
Sbjct: 133 ------------------------STSSTHDSIM-------------------------- 192

Query: 202 RLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEDST 261
               VG               D ++  +G+G  +PPT  ++K E +R   Y  SP ED +
Sbjct: 193 ---TVGE-------------EDIWEYIYGEGADKPPTGVLMKEEFRRHYYYSQSPLEDVS 252

Query: 262 LASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVY 321
           LAS LLRP P+ AL  A     N E ++V RVYI+T  D +  P +Q+ ++ +W P  +Y
Sbjct: 253 LASKLLRPAPVRALGGADKLSPNPEAEKVPRVYIKTAKDNLFDPLRQDRLVEKWPPSQLY 259

Query: 322 EM-DTDHSPFFSNPSLLFGLLVKAAA 347
            + ++DHS FFS P+ LF  L++A +
Sbjct: 313 ILEESDHSAFFSVPTTLFAYLLRAVS 259

BLAST of Carg02168 vs. ExPASy Swiss-Prot
Match: O23512 (Probable pheophorbidase OS=Arabidopsis thaliana OX=3702 GN=PPD PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.6e-45
Identity = 109/326 (33.44%), Postives = 153/326 (46.93%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG S G WCWYK+  L++ +GFK T +DL GAGI L D N V   D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNVNKF 141
           +S LP + ++ILVGHS GG SVT+A  KF  KI +A+Y+AA+M++ G      + N    
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPSPHLSN---- 131

Query: 142 FFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVWNIFRGFVRKKVKI 201
                 IH+                                                   
Sbjct: 132 ------IHVGEE------------------------------------------------ 191

Query: 202 RLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEDST 261
                                D ++  +G+G  +PPT  ++K E  R   Y  SP ED T
Sbjct: 192 ---------------------DIWEYTYGEGTDKPPTGVLMKPEFIRHYYYSQSPLEDVT 251

Query: 262 LASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVY 321
           L+S LLRP P+ A +       N E ++V RVYI+T  D +    +Q+ ++  W P  +Y
Sbjct: 252 LSSKLLRPAPMRAFQDLDKLPPNPEAEKVPRVYIKTAKDNLFDSVRQDLLVENWPPSQLY 258

Query: 322 EM-DTDHSPFFSNPSLLFGLLVKAAA 347
            + D+DHS FFS P+ LF  L++A +
Sbjct: 312 VLEDSDHSAFFSVPTTLFAYLLRAVS 258

BLAST of Carg02168 vs. ExPASy Swiss-Prot
Match: Q9FVW3 (Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES14 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.9e-34
Identity = 97/340 (28.53%), Postives = 155/340 (45.59%), Query Frame = 0

Query: 9   PSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSV 68
           P    S+ V+K   FVLVHG   G WCWYK+   +E SG     +DL G G +++D N+V
Sbjct: 87  PDFSESLIVKK---FVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTV 146

Query: 69  VKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFG 128
              ++Y+KPL+D + +LPE E++ILVGHS GG S++ A  +F +KI  A++V ATM    
Sbjct: 147 STLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATM---- 206

Query: 129 FQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVW 188
                                     + D   P  +    L +    +  S   IY    
Sbjct: 207 --------------------------VSDGQRPFDVFSEELGSAERFMKESQFLIY---- 266

Query: 189 NIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQR 248
                                                    G G  +PPT  + ++   +
Sbjct: 267 -----------------------------------------GNGKDKPPTGFMFEKPHMK 326

Query: 249 KIIYHMSPHEDSTLASMLLRPGPI--LALRTARFREENGELDRVKRVYIRTTYDRVIKPE 308
            + ++ SP++D  LA + +RP P+  +  + +   E  G   + +R Y++T  DR + P+
Sbjct: 327 GLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYG---KGRRFYVQTLDDRALSPD 345

Query: 309 QQEAMIREWLPEIVYEM-DTDHSPFFSNPSLLFGLLVKAA 346
            QE ++RE  PE V+++  +DH PFFS P  L  +L++ A
Sbjct: 387 VQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIA 345

BLAST of Carg02168 vs. ExPASy TrEMBL
Match: A0A6J1FL47 (methylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111444979 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 2.2e-146
Identity = 281/358 (78.49%), Postives = 281/358 (78.49%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180
           AATMLRFGFQTDQDIKN                                           
Sbjct: 121 AATMLRFGFQTDQDIKN------------------------------------------- 180

Query: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240
                                            GVPDLSEFGDAYDVGFGQGPQQPPTTA
Sbjct: 181 ---------------------------------GVPDLSEFGDAYDVGFGQGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 300
           VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD
Sbjct: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 281

Query: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN 359
           RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK AAAAAAASSTAACN
Sbjct: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK-AAAAAAASSTAACN 281

BLAST of Carg02168 vs. ExPASy TrEMBL
Match: A0A6J1JV68 (methylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111489169 PE=4 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.8e-144
Identity = 276/358 (77.09%), Postives = 277/358 (77.37%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180
           AATMLRFGFQTDQDIKN                                           
Sbjct: 121 AATMLRFGFQTDQDIKN------------------------------------------- 180

Query: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240
                                            GVPDLSEFGD YDVGFG GPQQPPTTA
Sbjct: 181 ---------------------------------GVPDLSEFGDVYDVGFGLGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYD 300
           V+KRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELD VKRVYIRTTYD
Sbjct: 241 VIKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDTVKRVYIRTTYD 280

Query: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASSTAACN 359
           RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK  AAAAAASSTAACN
Sbjct: 301 RVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK--AAAAAASSTAACN 280

BLAST of Carg02168 vs. ExPASy TrEMBL
Match: A0A5A7TBF5 (Methylesterase 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001670 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 6.4e-122
Identity = 229/348 (65.80%), Postives = 251/348 (72.13%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV++  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSV+ FDDYN+PLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180
           AATMLRFGFQTDQDIK+                                           
Sbjct: 121 AATMLRFGFQTDQDIKD------------------------------------------- 180

Query: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240
                                            GVPDLSE+GD Y++ FG GPQQPPTTA
Sbjct: 181 ---------------------------------GVPDLSEYGDIYNLEFGLGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENG--ELDRVKRVYIRTT 300
           V+KRE QRK+ YHMSPHEDSTLA+MLLRPGPI AL +A+F+EE    E D+VKRVYIRT 
Sbjct: 241 VIKREFQRKVAYHMSPHEDSTLAAMLLRPGPIQALMSAQFKEEEDREEADKVKRVYIRTM 272

Query: 301 YDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAA 347
           YDRVIKPEQQEAMI+ W PEIVYEMDTDHSPFFSNPSLLFGLL+K+ +
Sbjct: 301 YDRVIKPEQQEAMIKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCS 272

BLAST of Carg02168 vs. ExPASy TrEMBL
Match: A0A1S3CAT2 (methylesterase 17 OS=Cucumis melo OX=3656 GN=LOC103498605 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 6.4e-122
Identity = 229/348 (65.80%), Postives = 251/348 (72.13%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV++  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSV+ FDDYN+PLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180
           AATMLRFGFQTDQDIK+                                           
Sbjct: 121 AATMLRFGFQTDQDIKD------------------------------------------- 180

Query: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240
                                            GVPDLSE+GD Y++ FG GPQQPPTTA
Sbjct: 181 ---------------------------------GVPDLSEYGDIYNLEFGLGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENG--ELDRVKRVYIRTT 300
           V+KRE QRK+ YHMSPHEDSTLA+MLLRPGPI AL +A+F+EE    E D+VKRVYIRT 
Sbjct: 241 VIKREFQRKVAYHMSPHEDSTLAAMLLRPGPIQALMSAQFKEEEDREEADKVKRVYIRTM 272

Query: 301 YDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAA 347
           YDRVIKPEQQEAMI+ W PEIVYEMDTDHSPFFSNPSLLFGLL+K+ +
Sbjct: 301 YDRVIKPEQQEAMIKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCS 272

BLAST of Carg02168 vs. ExPASy TrEMBL
Match: A0A6J1BYB8 (methylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111006571 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 6.0e-120
Identity = 231/351 (65.81%), Postives = 253/351 (72.08%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60
           MGE++NLAPSAV SVAVQKP    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEEDNLAPSAV-SVAVQKPLPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGID SDPNSV+KFDDYNKPLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRL
Sbjct: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIML 180
           AVYVAATML+ G+QTDQDIK+                                       
Sbjct: 121 AVYVAATMLKLGYQTDQDIKD--------------------------------------- 180

Query: 181 NNSGSKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQP 240
                                                GVPDLSEFGD YD+ FG G QQP
Sbjct: 181 -------------------------------------GVPDLSEFGDVYDLEFGLGSQQP 240

Query: 241 PTTAVVKRELQRKIIYHMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIR 300
           PTTA++KRE QRKI YHMSPHEDSTL +MLLRPGPILAL+TA+FREE    D+VKRV+IR
Sbjct: 241 PTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPILALKTAQFREEEEFADKVKRVFIR 274

Query: 301 TTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAA 348
           T YDRVIK EQQ+AMI+ W PEIVY+MDTDHSPFFSNPSLLFGLL+KAAA+
Sbjct: 301 TMYDRVIKREQQDAMIKRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAAS 274

BLAST of Carg02168 vs. TAIR 10
Match: AT3G10870.1 (methyl esterase 17 )

HSP 1 Score: 319.3 bits (817), Expect = 3.9e-87
Identity = 161/336 (47.92%), Postives = 211/336 (62.80%), Query Frame = 0

Query: 18  QKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKP 77
           +KPPHFVL+HG+S G WCWYKI+CLME SGF VTCIDLK +GID S  +S+  FD YN+P
Sbjct: 16  RKPPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQYNQP 75

Query: 78  LLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKN 137
           L+DF+SS PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+ML+ G QTD+D+K+
Sbjct: 76  LIDFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDEDMKD 135

Query: 138 VNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVWNIFRGFVRK 197
                                                                       
Sbjct: 136 ------------------------------------------------------------ 195

Query: 198 KVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPH 257
                           GVPDLSE GD Y++GFG GP+ PPT+A++K E +RK++YHMSP 
Sbjct: 196 ----------------GVPDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQ 255

Query: 258 EDSTLASMLLRPGPILALRTARFRE---ENGELDRVKRVYIRTTYDRVIKPEQQEAMIRE 317
           ++ +LA++++RP PILAL TA+  E   E G+ ++V RVYI+T  DRV+KPEQQ+AMIR 
Sbjct: 256 QECSLAALMMRPAPILALTTAKLEEEEKEKGQEEQVPRVYIKTLLDRVMKPEQQDAMIRR 275

Query: 318 WLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAA 351
           W P  VYE+++DHSPFFSNP +LFGLL+KAA +  +
Sbjct: 316 WPPSQVYELESDHSPFFSNPFVLFGLLIKAAVSVGS 275

BLAST of Carg02168 vs. TAIR 10
Match: AT5G58310.1 (methyl esterase 18 )

HSP 1 Score: 195.3 bits (495), Expect = 8.5e-50
Identity = 118/327 (36.09%), Postives = 163/327 (49.85%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG   GGWCWYK+   + ++G K TCIDLKGAGI+ +DPN+V   DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNVNKF 141
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M++ G    + +KNV K 
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMK- 124

Query: 142 FFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVWNIFRGFVRKKVKI 201
                        IC                S ++     KI                  
Sbjct: 125 -------------IC----------------SGLIEEETEKI------------------ 184

Query: 202 RLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEDST 261
                                  +D  FG GPQ  PT+ ++K E  R   Y+ SP ED T
Sbjct: 185 -----------------------WDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPMEDYT 244

Query: 262 LASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVY 321
           LA+ LLRP P++A           E D++ RVY++T  D + +P  QE M+  W P   +
Sbjct: 245 LATTLLRPAPVMAFIGIMDIPGAPETDKIPRVYVKTGKDHLFEPVLQEVMLALWPPAHTF 260

Query: 322 EM-DTDHSPFFSNPSLLFGLLVKAAAA 348
            + D+DHS FFS P  L+  L++AA++
Sbjct: 305 LLPDSDHSAFFSQPQELYQFLLQAASS 260

BLAST of Carg02168 vs. TAIR 10
Match: AT4G16690.1 (methyl esterase 16 )

HSP 1 Score: 184.9 bits (468), Expect = 1.1e-46
Identity = 109/326 (33.44%), Postives = 153/326 (46.93%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG S G WCWYK+  L++ +GFK T +DL GAGI L D N V   D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNVNKF 141
           +S LP + ++ILVGHS GG SVT+A  KF  KI +A+Y+AA+M++ G      + N    
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPSPHLSN---- 131

Query: 142 FFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVWNIFRGFVRKKVKI 201
                 IH+                                                   
Sbjct: 132 ------IHVGEE------------------------------------------------ 191

Query: 202 RLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEDST 261
                                D ++  +G+G  +PPT  ++K E  R   Y  SP ED T
Sbjct: 192 ---------------------DIWEYTYGEGTDKPPTGVLMKPEFIRHYYYSQSPLEDVT 251

Query: 262 LASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVY 321
           L+S LLRP P+ A +       N E ++V RVYI+T  D +    +Q+ ++  W P  +Y
Sbjct: 252 LSSKLLRPAPMRAFQDLDKLPPNPEAEKVPRVYIKTAKDNLFDSVRQDLLVENWPPSQLY 258

Query: 322 EM-DTDHSPFFSNPSLLFGLLVKAAA 347
            + D+DHS FFS P+ LF  L++A +
Sbjct: 312 VLEDSDHSAFFSVPTTLFAYLLRAVS 258

BLAST of Carg02168 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 146.7 bits (369), Expect = 3.5e-35
Identity = 97/340 (28.53%), Postives = 155/340 (45.59%), Query Frame = 0

Query: 9   PSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSV 68
           P    S+ V+K   FVLVHG   G WCWYK+   +E SG     +DL G G +++D N+V
Sbjct: 87  PDFSESLIVKK---FVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTV 146

Query: 69  VKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFG 128
              ++Y+KPL+D + +LPE E++ILVGHS GG S++ A  +F +KI  A++V ATM    
Sbjct: 147 STLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATM---- 206

Query: 129 FQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVW 188
                                     + D   P  +    L +    +  S   IY    
Sbjct: 207 --------------------------VSDGQRPFDVFSEELGSAERFMKESQFLIY---- 266

Query: 189 NIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQR 248
                                                    G G  +PPT  + ++   +
Sbjct: 267 -----------------------------------------GNGKDKPPTGFMFEKPHMK 326

Query: 249 KIIYHMSPHEDSTLASMLLRPGPI--LALRTARFREENGELDRVKRVYIRTTYDRVIKPE 308
            + ++ SP++D  LA + +RP P+  +  + +   E  G   + +R Y++T  DR + P+
Sbjct: 327 GLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYG---KGRRFYVQTLDDRALSPD 345

Query: 309 QQEAMIREWLPEIVYEM-DTDHSPFFSNPSLLFGLLVKAA 346
            QE ++RE  PE V+++  +DH PFFS P  L  +L++ A
Sbjct: 387 VQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIA 345

BLAST of Carg02168 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-32
Identity = 98/330 (29.70%), Postives = 135/330 (40.91%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFVLVHG S G WCWYK   L+E  GFKVT IDL G GI+  + N +     Y KPL D 
Sbjct: 138 HFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSININGIASLSQYVKPLTDI 197

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNVNKF 141
           +  LP  E++ILVGH  GG  ++ A   F  KI  AV++AA ML  G  T          
Sbjct: 198 LEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAAMLTNGQST---------- 257

Query: 142 FFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSGSKIYFSVWNIFRGFVRKKVKI 201
                                                                       
Sbjct: 258 ------------------------------------------------------------ 317

Query: 202 RLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEDST 261
               + +  ++ G  DL      +   +  G + PPT   + + L + ++++ SP +D  
Sbjct: 318 ----LDMFSLKAGQNDLMRKAQIFI--YTNGNENPPTAIDLDKSLLKDLLFNQSPSKDVA 377

Query: 262 LASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVY 321
           LAS+ +R  P   +   +    +     V+R YI T  D  I    QE MI    PE VY
Sbjct: 378 LASVSMRSIPFAPV-LEKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKVY 390

Query: 322 EM-DTDHSPFFSNPSLLFGLLVKAAAAAAA 351
            +   DH+PFFS P  L  LL++ A  + A
Sbjct: 438 RLKGADHAPFFSKPQALHKLLLEIARISPA 390