Carg02143 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02143
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	alpha-soluble NSF attachment protein 2-like
Location	Carg_Chr15: 879533 .. 884030 (-)
RNA-Seq Expression	Carg02143
Synteny	Carg02143

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCTTCCTTGGACCAACAAAGCCCATAGTTTGACCGTACTAGTTTCCCCTTAATTTCTCTCCCTGTTTATACTCCTCGTTTAATTCCTCAAGCTCGAAGCAAACTCGAGGATTCTGTTTAAAGATGGCCGATCAGCTTGCTAAGGGAGAGGAATTCGAGAAGAAAGCGGAGAAGAAGCTTAATGGTTGGGGATTATTCGGTTCCAAGTACGAAGATGCCGCTGATCTCTTCGATAAGGCTGCTAACTCTTACAAGCTTGCTAAATCATGTAATTCGCCAAATCATTCTCTGCCAGTACTTTTTTTTTTCTTTTGGAGTCCTCTAGATCTTTGTGCGATGCGACTGATTCAATATTTCATGCCCCAACGCATCAACTTGGACTCAACTTTATTGTCTGTTGCTTGAATTAAAAAGGAATTTAAGAGCTGCATACTGATTCTTTTGTTGGAATTTGACCTACAATATTTCTTATACTGAAATTTGAATTTATTTGTTGATTCTGCTTGCAAGGTATGCACCTGCTTTGAACTAGGAGGGTTAAGATTAATGATCTGAAGAATAAATAAGATACTTTTTTGGCAATAAATGCATTTATTTATAGTCAGTTTATGGATGCAACTGCGTTTCCCGTGAGAAAATATGAACTGTTGTCTAGGAATGATGGACGGACACTGGATAAAAAGATTTGGATGTGTTAGCTATCTCAATATCGAATTTGGAGAGTATTGTTTGGGAATGAGTTACAAAAACTGAAAACTATAAGCAGAAGTTCATTAATGGTATAGCCATCCAGTGAGCGAAGGACATAGTTAAAGTTAAATGGTTTGGTTGCAAGATATATCATCCAAATGGATGAATAAGTATGAAGTGAAATTGTTCTTCATATGTTAGTTAAAGTCTCCATATGGATGATGAGATATTGACATTGACGAATTAGATATTCTGAATTCTGTTTTTGGATTTTTCTTAATATTTAAAGGGATTTGCCTGTCGACCCATTAGAGGATATATATATTCGTAATCCCTGGGATCTCTTCAGATTTGCTTTTATACAATACTGTCAAAACCAGGCATGAATTTCTTTGGTTCCCTACGTAGAAGCCTGCTGAACCATCAAAGATTTTTTGAACTAGTTGATCAACACTACTCATTTTAGTATATATTTGAATTCCTCTCTCTTCGATTCCTTCTAATTCAATTTTAGCGGCATTAATCCCTTTGCCCTGTGCATTAGAAAGGATTTCAAAACAGTCATGGTCTCCTAATTTTGTAGTCTAATGATTTTGCTGTCATTCACAATTACCTTACCTTTTCTCACTATGATACCTGTTATGTGGCTTTAATCTCTATGCATGCGGTGTTTTAGGGGATAAAGCTGGGTCAACATACATAAAATTAGCAAGTTGCTATCTGAAGGTAAATGCTTCAAGTGTGTGATAATGCCTATTCCTCATATCTTACCTTCTCTATCGTATTTTTAAAAATGATTTTTTTATAGGTGGAAAGTAAGCATGAAGCTGCTCAAGCTTATACGGATGCTGCCAATGCCTATAAGAAGACATCTATAAAGGGTAAGTCTAAATTTATAATATATCTTGATTGTAGCATGTCATGGGATTATTAAGCCTACATTGTTGATTCATTATATTTCTACTAGATGACATTACACTGGTAGAATTCCTCATCTTACACAGTCCATCTTCATCTGAACATTCACATTCACAACTTTATCAATGGCATTCCAATATTTTCTGTTCTCAAATACTTAATAACGAAAATCACTTCTCAATTACTCATAGAAGGGGGTTGATCAGTTTTCCCAACTCTTGATTTTTTTGTGAACACGTACACTATTCAACTATGTGACCAACTCCACTTGCCTTCTACGTTCTATGTTTTTCTAATGCTCCCTGCTTTCTTCATTAACTTGTTTGCTTATTTTTCAAATAAAAGAGATTGGGATGACTCATGATGCTCTGTAACACGAATTTCAGGTTCTGTTGGAATTGGACCTCCTTTATATGAAAAATCAATTTCTGATAGCATGAATAGAAATCCAAATGGAGAGAATGAAAAACTAACATAACACTACCTCCACATGTTAGATGGTTTGTTAGAATTGGCTTTTCTTTTAGTTTTATTTCTCTGTCCAAATATTCTTGCTCATGAGTGAATCTTTTGAAAATTGTCTTATTTTATTTTAATTTTTTCTTTACCTGATATTAGTTCTCTCTGTTAATATAAGTTGCCAACTCAGCAGGACGCAAGAAATAGCTCTCAGGTTCTCATATTTTCTCGTCAATAATGTAAATCCAGATATATTTTTGTGTTGGCTTAACCTGTTTAAAATTTAAATCATCTGCTATAATTAACCATGTTATTCACCATTATGTTGGAAGTCTAGAAATTTGTTGAAGCTAATTTTTTTTTGTTGTATTGTAGAGGCCATTTCATGCATGGAGCAGGCAGTAAATCTGTTTTGTGATATTGGAAGGCTCAGTATGGCTGCAAGATATTTGAAGGTGCATGCTTATGATACCTAAACTCAGCTCTTTAGCTTGGCAGAATCGTTTAGGAAACTAATTACTCAATAGTGCCTTTGGCTTTCTTTTTATAGGGCCCCTTTCTTTTGCTTCTGTCTCTACTTTGCCTGTAAGGGATTGTTCAGGTTGTAATTATCGTTAGTTATGTAGGAAATTGCTGAACTATATGAATCAGACCAGAACATTGAACTTGCTATTGGTTACTTTGAGAAAGCTGCCGACTTTTTCCAAAACGAGGAAGTAACAACATCTGTGAACCAGTGCAAGCAGAAAGTGGCGCAGTTTGCTGCTCAGTTAGAACAGTAAGCATTTCTTCTTATACTATTTTTGTACACTTTCTATCTATTAATTATTACTCCTGAGTGGTCATGGATTGGTTAAGTTCTCAATCATATTTTGATGTTTTTGAAAGCAATTGACGTGGCTATTAATGTATACCTTGTGGTTAAACTCATGTACAAGGAAAAACTATTACACAATATCCTTAGTGTTTTTCTTAATAATATTTGTATTTCAGTGGGAAATATATCTAACAGTAATGTAAACAGAAATGATTTATATACTAAGGAAATTTATTTTTGAATTCTAAGCTTCAATAGTGCACATGAAGCCCTGTGTCCATGGTGTGGCTTCTATCTTTTAGTCGTGAGGTAGTTGGTAATCAACTGTGAATTTTCTGTAGATACGAAAAGGCAATTAAAATATATGAAGAGATTGCTCGACATTCACTCAAAAGTAACTTGCTAAAATATGGAGTGAAAGGACATCTTCTGAATGCTGGCTTATGTCAACTCTGCAAAGGTGATATTGTAGCCATCACTAAAGCATTGGAGGAATATCAGGTTCAATTCTCAGCCTTCAATCTTCATGGTGTATATGTACCTTATTGCAACTCTCATTTAAGTGTTCTGCATATTGTTTTACAGGATTTAGATCCAACTTTTTCTGGCACGCGCGAATGTAGACTTTTAGCTGTAAGTTGTTTAGATCTTCAATTGACGTTTTATTTACTGCTTCCGTTCTCATCGATATGCATTGTTACGTAAATTTCATGATTGAGGCAGATAATTATCATCTCTTTTCATTTAGTTTGATTGCAAGTTCAGCTACCTCGACATTTCCACTTGGATTTTTATTTCATTCTTGCATGTTATGTGTGGCTGTCTTCAGATTACTACGATTTCAATTAGATACTTCCTTGCATTTTGCTATCACTTTTTTTTTTACTTTTTTTTTTTTTTTTGGATTTTCAATCATTGTTCTCTCTTGAGCTGAAAAAGTGCTTTATCTGTCAGGATATTGCTAGTGCAATTGATGAAGAAGATGTTACGAAGTTTACTGACGTGGTTAAGGAGTTCGATAGCATGACCCCACTGGTGAGTATTTGTGACGTTACATTGCATTATAAACTCTGAAGTATCCAATTTGGTTGATAGAAATTGATAATACCGAGCGATTCTCGTGCTGAAAACTTCATGAGTGTCGCATAGATTAAGGTTTCTGCACTTTTAGCATAAGCATTCACGACTTTACTTTTGTAGAAAAAGCATTGAATGAAGATAATTATTATTACTAATATACCTTTATGATATTACGCTTGGTTTACCAGGACCCATGGAAGACAACTCTGCTGTTGAGAGTGAAGGAAAAGCTGAAAGCCAAGGAGATGGAGGAGGATGACCTTACCTAAATTTCTCCATTATAAATTGTTTTGTTGTGTCACGGTTTGTTGATTGCAGCACTATATATGATGAGTTTTATGTAAATTTTCACCACATTTGTAATGCCTGAGATTCTAAAAAATTACCTTGTTTCGGGCTCAGTTTGCCTGAGATTCAGTTTGCGTGCATGAGTTTGTGTAGTTTTCTATTCGAAACTATGCTTTCCTTTGACTTCGCTCGAATACCAATTTGAGATAATTGACAAATCCCAGCGTTAATTGTACTCCTTCCCT

mRNA sequence

AACCTTCCTTGGACCAACAAAGCCCATAGTTTGACCGTACTAGTTTCCCCTTAATTTCTCTCCCTGTTTATACTCCTCGTTTAATTCCTCAAGCTCGAAGCAAACTCGAGGATTCTGTTTAAAGATGGCCGATCAGCTTGCTAAGGGAGAGGAATTCGAGAAGAAAGCGGAGAAGAAGCTTAATGGTTGGGGATTATTCGGTTCCAAGTACGAAGATGCCGCTGATCTCTTCGATAAGGCTGCTAACTCTTACAAGCTTGCTAAATCATGGGATAAAGCTGGGTCAACATACATAAAATTAGCAAGTTGCTATCTGAAGGTGGAAAGTAAGCATGAAGCTGCTCAAGCTTATACGGATGCTGCCAATGCCTATAAGAAGACATCTATAAAGGAGGCCATTTCATGCATGGAGCAGGCAGTAAATCTGTTTTGTGATATTGGAAGGCTCAGTATGGCTGCAAGATATTTGAAGGAAATTGCTGAACTATATGAATCAGACCAGAACATTGAACTTGCTATTGGTTACTTTGAGAAAGCTGCCGACTTTTTCCAAAACGAGGAAGTAACAACATCTGTGAACCAGTGCAAGCAGAAAGTGGCGCAGTTTGCTGCTCAGTTAGAACAATACGAAAAGGCAATTAAAATATATGAAGAGATTGCTCGACATTCACTCAAAAGTAACTTGCTAAAATATGGAGTGAAAGGACATCTTCTGAATGCTGGCTTATGTCAACTCTGCAAAGGTGATATTGTAGCCATCACTAAAGCATTGGAGGAATATCAGGATTTAGATCCAACTTTTTCTGGCACGCGCGAATGTAGACTTTTAGCTGATATTGCTAGTGCAATTGATGAAGAAGATGTTACGAAGTTTACTGACGTGGTTAAGGAGTTCGATAGCATGACCCCACTGGACCCATGGAAGACAACTCTGCTGTTGAGAGTGAAGGAAAAGCTGAAAGCCAAGGAGATGGAGGAGGATGACCTTACCTAAATTTCTCCATTATAAATTGTTTTGTTGTGTCACGGTTTGTTGATTGCAGCACTATATATGATGAGTTTTATGTAAATTTTCACCACATTTGTAATGCCTGAGATTCTAAAAAATTACCTTGTTTCGGGCTCAGTTTGCCTGAGATTCAGTTTGCGTGCATGAGTTTGTGTAGTTTTCTATTCGAAACTATGCTTTCCTTTGACTTCGCTCGAATACCAATTTGAGATAATTGACAAATCCCAGCGTTAATTGTACTCCTTCCCT

Coding sequence (CDS)

ATGGCCGATCAGCTTGCTAAGGGAGAGGAATTCGAGAAGAAAGCGGAGAAGAAGCTTAATGGTTGGGGATTATTCGGTTCCAAGTACGAAGATGCCGCTGATCTCTTCGATAAGGCTGCTAACTCTTACAAGCTTGCTAAATCATGGGATAAAGCTGGGTCAACATACATAAAATTAGCAAGTTGCTATCTGAAGGTGGAAAGTAAGCATGAAGCTGCTCAAGCTTATACGGATGCTGCCAATGCCTATAAGAAGACATCTATAAAGGAGGCCATTTCATGCATGGAGCAGGCAGTAAATCTGTTTTGTGATATTGGAAGGCTCAGTATGGCTGCAAGATATTTGAAGGAAATTGCTGAACTATATGAATCAGACCAGAACATTGAACTTGCTATTGGTTACTTTGAGAAAGCTGCCGACTTTTTCCAAAACGAGGAAGTAACAACATCTGTGAACCAGTGCAAGCAGAAAGTGGCGCAGTTTGCTGCTCAGTTAGAACAATACGAAAAGGCAATTAAAATATATGAAGAGATTGCTCGACATTCACTCAAAAGTAACTTGCTAAAATATGGAGTGAAAGGACATCTTCTGAATGCTGGCTTATGTCAACTCTGCAAAGGTGATATTGTAGCCATCACTAAAGCATTGGAGGAATATCAGGATTTAGATCCAACTTTTTCTGGCACGCGCGAATGTAGACTTTTAGCTGATATTGCTAGTGCAATTGATGAAGAAGATGTTACGAAGTTTACTGACGTGGTTAAGGAGTTCGATAGCATGACCCCACTGGACCCATGGAAGACAACTCTGCTGTTGAGAGTGAAGGAAAAGCTGAAAGCCAAGGAGATGGAGGAGGATGACCTTACCTAA

Protein sequence

MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLASCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAELYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIARHSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIASAIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT

Homology

[Close]

BLAST of Carg02143 vs. NCBI nr
Match: KAG7016031.1 (Alpha-soluble NSF attachment protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 563.1 bits (1450), Expect = 1.3e-156
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 289

[Close]

BLAST of Carg02143 vs. NCBI nr
Match: XP_022939232.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita moschata] >XP_023551462.1 alpha-soluble NSF attachment protein 2-like [Cucurbita pepo subsp. pepo] >KAG6578465.1 Alpha-soluble NSF attachment protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 561.6 bits (1446), Expect = 3.9e-156
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYESDQNIELAIGYFEKAADFFQNEEVTTS NQCKQKVAQFAAQLEQYEKAIKIYEEIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 289

[Close]

BLAST of Carg02143 vs. NCBI nr
Match: XP_022992586.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita maxima])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 287/289 (99.31%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYES+QNIELAIGYFEKAADFFQNEEVTTS NQCKQKVAQFAAQLEQYEKAIKIYEEIAR
Sbjct: 121 LYESEQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 289

[Close]

BLAST of Carg02143 vs. NCBI nr
Match: XP_023515361.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545.4 bits (1404), Expect = 2.9e-151
Identity = 279/289 (96.54%), Postives = 282/289 (97.58%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKA KKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAVKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYTKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            C+LKVESKHEAAQAY DAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  GCHLKVESKHEAAQAYVDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYESDQNIELAIGYFEKAAD FQNEEVTTS NQCKQKVAQFAAQLEQYEKAIKIYEEIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKAL+EYQDLDPTFSGTRECRLLADIA 
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQDLDPTFSGTRECRLLADIAG 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of Carg02143 vs. NCBI nr
Match: KAG6602599.1 (Alpha-soluble NSF attachment protein 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033278.1 Alpha-soluble NSF attachment protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 543.9 bits (1400), Expect = 8.4e-151
Identity = 278/289 (96.19%), Postives = 282/289 (97.58%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKA KKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAVKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYTKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            C+LKVESKHEAAQAY DAANAYKKTSIKEAISC+EQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  GCHLKVESKHEAAQAYVDAANAYKKTSIKEAISCIEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYESDQNIELAIGYFEKAAD FQNEEVTTS NQCKQKVAQFAAQLEQYEKAIKIYEEIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKAL+EYQDLDPTFSGTRECRLLADIA 
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQDLDPTFSGTRECRLLADIAG 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of Carg02143 vs. ExPASy Swiss-Prot
Match: Q9SPE6 (Alpha-soluble NSF attachment protein 2 OS=Arabidopsis thaliana OX=3702 GN=ASNAP2 PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 5.5e-129
Identity = 226/289 (78.20%), Postives = 254/289 (87.89%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           M D L + EEFEKKAEKKLNGWG+FGSKYEDAADL +KAANSYKLAKSWD+AG  Y+KLA
Sbjct: 1   MGDHLVRAEEFEKKAEKKLNGWGIFGSKYEDAADLLEKAANSYKLAKSWDQAGKAYLKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            C+LK +SKH+AA AY +AA  YKK    EA SC+E+AVN+FC+IGRL+MAARY KEIAE
Sbjct: 61  DCHLKSDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNMAARYYKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
            YESDQ  E AI YFEKAA+FFQNEEVTTS NQC  KVAQ+AAQLEQYEKAIKIYE+IAR
Sbjct: 121 YYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEKAIKIYEDIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSL +NLLKYGVKGHLL AG+C LCK D+V+IT ALE+YQDLDPTF+GTREC+ LAD+AS
Sbjct: 181 HSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTRECKFLADLAS 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEED+ KFTDVVKEFDSMTPLD WKTT+LLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of Carg02143 vs. ExPASy Swiss-Prot
Match: P93798 (Alpha-soluble NSF attachment protein OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 4.7e-120
Identity = 214/289 (74.05%), Postives = 248/289 (85.81%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MAD + +GEEFEKKAEKK++GWGLFGSKYEDAAD +DKAAN +KLAKSWD+AGSTY+KL+
Sbjct: 1   MADNIQRGEEFEKKAEKKISGWGLFGSKYEDAADFYDKAANCFKLAKSWDRAGSTYVKLS 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           +     +SKHEAAQAY DA + YKKTS KEAISC+EQA  LF D GR +MA +Y KEIAE
Sbjct: 61  TVIQSSDSKHEAAQAYADAGHCYKKTSAKEAISCLEQAAYLFLDNGRFNMAGKYYKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYE +QN E AI YFEKAAD +Q+EE TT+ NQC  KVAQFAAQLEQY+KAI+IYE+I R
Sbjct: 121 LYELEQNFEQAIIYFEKAADIYQSEEATTAANQCNAKVAQFAAQLEQYQKAIQIYEDIGR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
            SL +NLLKYGVKGHLLNAG+CQLCKGD+VAIT AL+ YQ++DPTFSGTRE +LL D+A+
Sbjct: 181 PSLNNNLLKYGVKGHLLNAGICQLCKGDVVAITNALDRYQEMDPTFSGTREYKLLVDLAA 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           A+DEEDV KFTD VKEFDSMT LD WKTTLLLRVKE +KAKE+EEDDLT
Sbjct: 241 AVDEEDVVKFTDAVKEFDSMTQLDAWKTTLLLRVKEAIKAKELEEDDLT 289

[Close]

BLAST of Carg02143 vs. ExPASy Swiss-Prot
Match: Q9M5P8 (Alpha-soluble NSF attachment protein OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 6.6e-114
Identity = 204/289 (70.59%), Postives = 246/289 (85.12%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           M D +A+GE+FE KAEKKL GW LFGSKYEDAADLFDKA N +KLAKSWDKAG+ Y+KLA
Sbjct: 1   MGDHIARGEDFENKAEKKLGGWALFGSKYEDAADLFDKAGNCFKLAKSWDKAGAVYVKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           +C+LK++SKHEAA AY DAA+ YKK++IKEA+SC+EQAVN F DIGRL+M+ARY KEIAE
Sbjct: 61  NCHLKLDSKHEAANAYADAAHCYKKSNIKEAVSCLEQAVNFFLDIGRLNMSARYYKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYE +QN++ AI Y     +   +E+V+   NQC +K+AQF+AQ E+Y KAI+I+EEIAR
Sbjct: 121 LYEQEQNLDQAIRYAVYEIN-PGSEDVSAPPNQCLKKIAQFSAQNEKYPKAIEIFEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HS+ +NLLKYGV+ HLLNAG+CQLCKGD+V I  ALE YQ+LDPTFSGTREC+LL D+A+
Sbjct: 181 HSVNNNLLKYGVREHLLNAGICQLCKGDVVPINNALERYQELDPTFSGTRECKLLVDLAA 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDV KFTD VKE+DSMT LD W+TTLLLRVKE LKAKE+EEDDLT
Sbjct: 241 AIDEEDVAKFTDAVKEYDSMTQLDAWRTTLLLRVKETLKAKELEEDDLT 288

[Close]

BLAST of Carg02143 vs. ExPASy Swiss-Prot
Match: Q9LXZ5 (Alpha-soluble NSF attachment protein 1 OS=Arabidopsis thaliana OX=3702 GN=ASNAP1 PE=1 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.1e-68
Identity = 146/253 (57.71%), Postives = 171/253 (67.59%), Query Frame = 0


Query: 11  FEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLASCYLKVESKH 70
           F+KKAEKKLN    F +KYEDAADL +KA +SYKLAKSWD+AG  Y+KLA C+LK  S  
Sbjct: 99  FQKKAEKKLNRRRRFDTKYEDAADLLEKARDSYKLAKSWDQAGIAYLKLADCHLKANS-- 158

Query: 71  EAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAELYESDQNIEL 130
                    AN Y          CM+                    EIAE YESD+  E 
Sbjct: 159 --------LANTYMIMD-----ECMDH-------------------EIAEYYESDEMFEQ 218

Query: 131 AIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIARHSLKSNLLKY 190
           AI Y+E AA+FFQ EEVTTS NQC  KVAQ+A+QLEQ  + +K     ARHSL + LLKY
Sbjct: 219 AIAYYETAAEFFQIEEVTTSANQCNLKVAQYASQLEQQSRFMKTQ---ARHSLNNKLLKY 278

Query: 191 GVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIASAIDEEDVTKF 250
           GVKGHLL AG+C LCK D+V+IT ALE+YQDLDPTFSGTREC+ LAD+ASAIDEED+ KF
Sbjct: 279 GVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFSGTRECKFLADLASAIDEEDIAKF 314

Query: 251 TDVVKEFDSMTPL 264
           TDV KE DS++PL
Sbjct: 339 TDVSKEIDSVSPL 314

[Close]

BLAST of Carg02143 vs. ExPASy Swiss-Prot
Match: Q54NP6 (Alpha-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snpA PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.4e-63
Identity = 125/279 (44.80%), Postives = 189/279 (67.74%), Query Frame = 0


Query: 6   AKGEEFEKKAEKKLNGWGLF------GSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKL 65
           AK +EF   A+K+L G   F       S+Y+DAA  + KAAN +K++K WD+AG+ + K 
Sbjct: 5   AKAKEFLDAADKRLRGGNFFKMFGGGSSRYDDAASDYTKAANLFKMSKKWDQAGAAFQKA 64

Query: 66  ASCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIA 125
           A C+LK  SKH+AA +Y  AA  YKK ++ +AI+C++ A+  + D GR +++A++ KEIA
Sbjct: 65  AECFLKGSSKHDAASSYVLAAGCYKKGNVIDAITCLKAAIEYYTDEGRFAISAKHQKEIA 124

Query: 126 ELYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIA 185
           ELYE++ + + AI  ++ A+D+F  E  T S +QC  K+A F+AQLE+YEK+I+IYE++A
Sbjct: 125 ELYEAEGDFDQAIASYQIASDYFDGENSTVSSHQCLLKIALFSAQLERYEKSIEIYEQVA 184

Query: 186 RHSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIA 245
             SL +NL ++G K + L A LC L   D+V   +AL+ Y+D+  +F+ TRECRLL  I 
Sbjct: 185 AASLDNNLTQWGCKEYFLRACLCYLAADDVVGAERALQRYKDMQASFNSTRECRLLDGII 244

Query: 246 SAIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKL 279
            A    +V  FT+ V EF+S++PLD WKT++LLR+K  +
Sbjct: 245 QACRNNNVEDFTNEVAEFNSISPLDSWKTSILLRIKNTI 283

[Close]

BLAST of Carg02143 vs. ExPASy TrEMBL
Match: A0A6J1FG84 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111445208 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.9e-156
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYESDQNIELAIGYFEKAADFFQNEEVTTS NQCKQKVAQFAAQLEQYEKAIKIYEEIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 289

[Close]

BLAST of Carg02143 vs. ExPASy TrEMBL
Match: A0A6J1JTZ3 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488888 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.5e-156
Identity = 287/289 (99.31%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYES+QNIELAIGYFEKAADFFQNEEVTTS NQCKQKVAQFAAQLEQYEKAIKIYEEIAR
Sbjct: 121 LYESEQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 289

[Close]

BLAST of Carg02143 vs. ExPASy TrEMBL
Match: A0A6J1JTS3 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487775 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.2e-150
Identity = 277/289 (95.85%), Postives = 281/289 (97.23%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKA KKLNGWGLFGSKYEDAADLFDK+ANSYKLAKSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAVKKLNGWGLFGSKYEDAADLFDKSANSYKLAKSWDKAGSTYTKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            C+LKVESKHEAAQAY DAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  GCHLKVESKHEAAQAYVDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYESDQNIELAIGYFEKAAD FQNEEVTTS NQCKQKVAQFAAQLEQYEKAIKIYEEIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKAL+EYQDLDPTFSGTRECRLLAD A 
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQDLDPTFSGTRECRLLADTAG 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of Carg02143 vs. ExPASy TrEMBL
Match: A0A6J1HIF0 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464692 PE=3 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 4.5e-150
Identity = 278/289 (96.19%), Postives = 281/289 (97.23%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKA KKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAVKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYTKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            C+LKVESKHEAAQAY DAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  GCHLKVESKHEAAQAYVDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYESDQNIELAIGYFEKAAD FQNEEVTTS NQCKQKVAQFAAQLEQYEKAIKIYEEIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKAL+EYQDLDPTFSGTRECRLLADIA 
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQDLDPTFSGTRECRLLADIAG 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDVTKFTDVVKEFDSMTPLDP KTTLLLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDVTKFTDVVKEFDSMTPLDPCKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of Carg02143 vs. ExPASy TrEMBL
Match: A0A6J1BXG4 (alpha-soluble NSF attachment protein 2 OS=Momordica charantia OX=3673 GN=LOC111006586 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.3e-149
Identity = 275/289 (95.16%), Postives = 281/289 (97.23%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAAN YKL+KSWDKAGSTY KLA
Sbjct: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANCYKLSKSWDKAGSTYTKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
           SC+LKVESKHEAAQAY DAAN+YKKTSIKEA SCMEQAVNLFCDIGRLSMAARYLKEIAE
Sbjct: 61  SCHLKVESKHEAAQAYVDAANSYKKTSIKEATSCMEQAVNLFCDIGRLSMAARYLKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
           LYESDQNIELAIGYFEKAADFFQNEEVTTS NQCKQKVAQFAAQLEQYEKAIKIY EIAR
Sbjct: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYEKAIKIYVEIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKAL+EYQ+LDPTFS TRECRLLADIAS
Sbjct: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALDEYQELDPTFSNTRECRLLADIAS 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEEDV KFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDVAKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of Carg02143 vs. TAIR 10
Match: AT3G56190.1 (alpha-soluble NSF attachment protein 2 )

HSP 1 Score: 461.8 bits (1187), Expect = 3.9e-130
Identity = 226/289 (78.20%), Postives = 254/289 (87.89%), Query Frame = 0


Query: 1   MADQLAKGEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLA 60
           M D L + EEFEKKAEKKLNGWG+FGSKYEDAADL +KAANSYKLAKSWD+AG  Y+KLA
Sbjct: 1   MGDHLVRAEEFEKKAEKKLNGWGIFGSKYEDAADLLEKAANSYKLAKSWDQAGKAYLKLA 60

Query: 61  SCYLKVESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAE 120
            C+LK +SKH+AA AY +AA  YKK    EA SC+E+AVN+FC+IGRL+MAARY KEIAE
Sbjct: 61  DCHLKSDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNMAARYYKEIAE 120

Query: 121 LYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIAR 180
            YESDQ  E AI YFEKAA+FFQNEEVTTS NQC  KVAQ+AAQLEQYEKAIKIYE+IAR
Sbjct: 121 YYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEKAIKIYEDIAR 180

Query: 181 HSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIAS 240
           HSL +NLLKYGVKGHLL AG+C LCK D+V+IT ALE+YQDLDPTF+GTREC+ LAD+AS
Sbjct: 181 HSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTRECKFLADLAS 240

Query: 241 AIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           AIDEED+ KFTDVVKEFDSMTPLD WKTT+LLRVKEKLKAKE+EEDDLT
Sbjct: 241 AIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 289

[Close]

BLAST of Carg02143 vs. TAIR 10
Match: AT3G56190.2 (alpha-soluble NSF attachment protein 2 )

HSP 1 Score: 357.5 bits (916), Expect = 1.0e-98
Identity = 180/239 (75.31%), Postives = 207/239 (86.61%), Query Frame = 0


Query: 53  GSTYIKLA-SCYLKV-ESKHEAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSM 112
           GS++  L+ +C L + +SKH+AA AY +AA  YKK    EA SC+E+AVN+FC+IGRL+M
Sbjct: 2   GSSWKSLSKTCRLSLKDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNM 61

Query: 113 AARYLKEIAELYESDQNIELAIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEK 172
           AARY KEIAE YESDQ  E AI YFEKAA+FFQNEEVTTS NQC  KVAQ+AAQLEQYEK
Sbjct: 62  AARYYKEIAEYYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEK 121

Query: 173 AIKIYEEIARHSLKSNLLKYGVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTR 232
           AIKIYE+IARHSL +NLLKYGVKGHLL AG+C LCK D+V+IT ALE+YQDLDPTF+GTR
Sbjct: 122 AIKIYEDIARHSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTR 181

Query: 233 ECRLLADIASAIDEEDVTKFTDVVKEFDSMTPLDPWKTTLLLRVKEKLKAKEMEEDDLT 290
           EC+ LAD+ASAIDEED+ KFTDVVKEFDSMTPLD WKTT+LLRVKEKLKAKE+EEDDLT
Sbjct: 182 ECKFLADLASAIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 240

[Close]

BLAST of Carg02143 vs. TAIR 10
Match: AT3G56450.1 (alpha-soluble NSF attachment protein 1 )

HSP 1 Score: 261.5 bits (667), Expect = 7.8e-70
Identity = 146/253 (57.71%), Postives = 171/253 (67.59%), Query Frame = 0


Query: 11  FEKKAEKKLNGWGLFGSKYEDAADLFDKAANSYKLAKSWDKAGSTYIKLASCYLKVESKH 70
           F+KKAEKKLN    F +KYEDAADL +KA +SYKLAKSWD+AG  Y+KLA C+LK  S  
Sbjct: 99  FQKKAEKKLNRRRRFDTKYEDAADLLEKARDSYKLAKSWDQAGIAYLKLADCHLKANS-- 158

Query: 71  EAAQAYTDAANAYKKTSIKEAISCMEQAVNLFCDIGRLSMAARYLKEIAELYESDQNIEL 130
                    AN Y          CM+                    EIAE YESD+  E 
Sbjct: 159 --------LANTYMIMD-----ECMDH-------------------EIAEYYESDEMFEQ 218

Query: 131 AIGYFEKAADFFQNEEVTTSVNQCKQKVAQFAAQLEQYEKAIKIYEEIARHSLKSNLLKY 190
           AI Y+E AA+FFQ EEVTTS NQC  KVAQ+A+QLEQ  + +K     ARHSL + LLKY
Sbjct: 219 AIAYYETAAEFFQIEEVTTSANQCNLKVAQYASQLEQQSRFMKTQ---ARHSLNNKLLKY 278

Query: 191 GVKGHLLNAGLCQLCKGDIVAITKALEEYQDLDPTFSGTRECRLLADIASAIDEEDVTKF 250
           GVKGHLL AG+C LCK D+V+IT ALE+YQDLDPTFSGTREC+ LAD+ASAIDEED+ KF
Sbjct: 279 GVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFSGTRECKFLADLASAIDEEDIAKF 314

Query: 251 TDVVKEFDSMTPL 264
           TDV KE DS++PL
Sbjct: 339 TDVSKEIDSVSPL 314

The following BLAST results are available for this feature:

BLAST of Carg02143 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016031.1	1.3e-156	100.00	Alpha-soluble NSF attachment protein 2 [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_022939232.1	3.9e-156	99.65	alpha-soluble NSF attachment protein 2-like [Cucurbita moschata] >XP_023551462.1...	[more]
XP_022992586.1	1.1e-155	99.31	alpha-soluble NSF attachment protein 2-like [Cucurbita maxima]	[more]
XP_023515361.1	2.9e-151	96.54	alpha-soluble NSF attachment protein 2-like [Cucurbita pepo subsp. pepo]	[more]
KAG6602599.1	8.4e-151	96.19	Alpha-soluble NSF attachment protein 2, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Carg02143 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SPE6	5.5e-129	78.20	Alpha-soluble NSF attachment protein 2 OS=Arabidopsis thaliana OX=3702 GN=ASNAP2...	[more]
P93798	4.7e-120	74.05	Alpha-soluble NSF attachment protein OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
Q9M5P8	6.6e-114	70.59	Alpha-soluble NSF attachment protein OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q9LXZ5	1.1e-68	57.71	Alpha-soluble NSF attachment protein 1 OS=Arabidopsis thaliana OX=3702 GN=ASNAP1...	[more]
Q54NP6	1.4e-63	44.80	Alpha-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snp...	[more]

BLAST of Carg02143 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FG84	1.9e-156	99.65	alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JTZ3	5.5e-156	99.31	alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1JTS3	1.2e-150	95.85	alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1HIF0	4.5e-150	96.19	alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1BXG4	1.3e-149	95.16	alpha-soluble NSF attachment protein 2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Carg02143 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56190.1	3.9e-130	78.20	alpha-soluble NSF attachment protein 2	[more]
AT3G56190.2	1.0e-98	75.31	alpha-soluble NSF attachment protein 2	[more]
AT3G56450.1	7.8e-70	57.71	alpha-soluble NSF attachment protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 151..178
None	No IPR available	PFAM	PF14938	SNAP	coord: 7..278 e-value: 2.0E-111 score: 372.0
None	No IPR available	PANTHER	PTHR13768:SF39	BNAA09G35650D PROTEIN	coord: 1..289
IPR000744	NSF attachment protein	PRINTS	PR00448	NSFATTACHMNT	coord: 28..47 score: 47.14 coord: 224..244 score: 38.78 coord: 255..275 score: 57.82 coord: 91..108 score: 34.92 coord: 152..175 score: 66.07 coord: 183..202 score: 41.43
IPR000744	NSF attachment protein	PANTHER	PTHR13768	SOLUBLE NSF ATTACHMENT PROTEIN SNAP	coord: 1..289
IPR000744	NSF attachment protein	CDD	cd15832	SNAP	coord: 23..278 e-value: 6.73674E-120 score: 343.022
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 5..288 e-value: 7.8E-108 score: 362.1
IPR011990	Tetratricopeptide-like helical domain superfamily	SUPERFAMILY	48452	TPR-like	coord: 7..281

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg02143-RA	Carg02143-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0035494	SNARE complex disassembly
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005483	soluble NSF attachment protein activity
molecular_function	GO:0019905	syntaxin binding
molecular_function	GO:0005515	protein binding