Carg02017 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02017
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncytochrome P450 714C3-like
LocationCarg_Chr04: 8020492 .. 8025844 (-)
RNA-Seq ExpressionCarg02017
SyntenyCarg02017
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGAAAGCGCTGAAGTTCTTACAGTTTAGTTCGCTTTCCTTTCCACCGATGAGAAAATGAGCTCGAAATGCGACGGCACTACTCCTCTCAACGTCATCAAAATACTTCTCCGATCGGAATCTTCCCAGAACTGTATATCCGAAGAGAAGTCCCGGATTCTTGGGGATTTCGTCACTCGCGAAGTCAATGCGTTCCTCTGGTTTTCTCTCATCGCCATCACTGTGGTTCTGATCGGCAAGGTTGTTGAACTCTTCAGATTGTGGTCGAAGGCGAAGCAACTTCCCGGACCTCCTAGTGCGTCTTTCTGCGGTTACTCCAAGGTCATTTCGCGCCGGAATCTCACGGGTGAGTTGTAAATTGAATGTGATCTATGCGTTTTTGGCTTGTTTGATTCTGAAGCATGTGGTTTTTATCGTGGAAGGAGAATGGATGGAAGAGGAGTTATGTTTTTCTTGATTTGCTTAGGCTGCTGCAAATTTTTTTTAGGATTCGATAAAATTGAATCTAAATTCCATTTGAGCGGTTATTGGATGCCGATTGGAGGCAACAACTCGTGTGTTAAAATTTGGAGCAACTGGACCGTTCCTGTACTGTTTATTGTTAAGAAATAAAATTCAGTTGAGATGAAATTTTTAAATGCTCCCGTGGGACAACAAAAAATAGCACGTCTGAATGGGAAGAGATGGTAAAAGGAAAGTAATAACAAAAATCCTCAGTGGCTCGTTCGCATCGAGTTGATCCAAAAGAATACCTTCTATTTGCTTCTGAACGGCGTAAGGGAGAGGGTAAGCCACGGCAGTTTTTCATTGGAGTGATATCGTTCTAATCAAATATCCGAAATAACTTCCAATAAGGTCCGAGTTTGTCAAGTGCAATCACGGTCAAGGAATGAATATCAGTTCTAATTATGAGGATCGAGGATCATATCCTGCTTCTAATTGCTTATATCAGTAAATTTTGTTTATTTACTTGGCCCCTCGTAAATCTGATCTTCCAAGATGGCATGGCATCTGGTATTTACTCATATTTCCTCTGAAATTGCTTTACTCTTTTGTCCTGTAGATCTATTATATGACTCACATAAAAAATATGGACCAGTCGTCAAGCTATGGTTGGGTCCCATGCAGCTTTTGGTGTCTGTAAAGGAGCCAGCTCTTCTCAAAGAAATTCTGGTAAAGGCCGAGGATAAATTGCTTTTGACAGGAAGGGCATTCAGATTGGCATTTGGGCGTTCAAGCCTCTTTACTTCCTCTTTAGAGAAGGTACAAGTCTTGTACTTCCCCTCTTGTGTTTTATTCAAATTTCTTGTAAGGCCATAAATGTCAGGGTATATAACTGATTGATTTTTGTGGGAGCAGAAATAGATGGTGTCTCTCATTCAATCATTTGATCAATAGGAAATATTATTATATTAGTATATTAGTATATTAGTATATTAGTAAAGATCGAGTCATTTGTTTGAGGAGAGTAATAATTTTCTTCTTCATATGATTTTTTTTCTTAATGTTTGAAGTATGAATTGCAATCTCATTAACCAGAGTCCTGTAGGCTGTAAGAATGTGCCTTTTGAGTTTTGATTTCTGTATGTTATGGGACCTAGTTACGTGTCGTGTCTGGAGGAGCTGCTCTAGGCAGCCATGTAATCATCTGTTTAGGGTGGAGGCCAGAACATATTAGATTGAATCCAATTTCTTCTTGTTTTCCTTATCATGTAATTATTTCATGGTGATATAAATGACATAATTGTGTTAAGACTCTTGAATTAGTGCTGATTATCTTATTTTTTGTCTCAAGTACAGGTGCAAAACAGAAGACGGTGGCTAGCAGAAAAGTTAGATGAAATATTGTTCCAGAGAGCTAATGTTTTTCCTGCAAAGGCTGTGGATTGTTCTGTAGGGAGAGTGCAAGATATTATGATTGAAGAAAGTGTAGATTGTAATAAGGTTTCTCAACATTTGGCTTTTACATTGTTAGGGTGCACACTTTTTGGGGATGCTTTTTTGGGTTGGTCCAAGGCAACCATCTATGAGGAACTTCTGATGATGGTTGCAAAAGATGCCAGCTTTTGGGCCTCCTACAGTGTTACTCCTTTCTGGAAACAAGGATTCTGGCGGTACCAGCGCTTGTGCATGAAATTGAAATGCTTAACACAAGATATTGTTCAGCAATGTAGAAAACATTGCAAGCTCTCCTCTCACTCCCAGAATCAGAACCTTCAAAATGAAATCAAAAGTACTGGTGTGGAGGCTGAATTTGTCATACCACGGTGTCCTGCTACGGAGATGCGTAATAGCTGTTTCTTTTATGGTCTTCATGATCAGGTTAATCCTAATGAAGAACCAATTGGAAATATTATGGGTGTGATGTTTCATGGATGTCTAACTACCGCAAATTTGATTGCTTCCATTTTGGGAAGGCTTGCCACAAATCCTGAAATACAAGAAAAGGTAATTTTTTTTGTTAAATTGTTCTATCAATTGATTTATGAGTCATTTTTCACATTAATGGACTAAATAGTTGGTATTCTAAAGAGTTGGAATAATTCATCTTCATACAGATCAACTCAGAACTAAATATAGTACAACAGGGCTCGGTGAAAGATCACCAGAAAAATGTTAATAACATGCCACTTCTGTTGGCAACTATATATGAATCTGCTCGTCTTCTGCCAGCAGGGCCTCTGTTACAAAGATGTTCACTCAAACAAGGTATCTCACTTCTTTTATAGCTTGGTGTGTACTGAGAATCTATAGCTGCTTTATGGAAAGTAGTAGTGCACTGGTCCTTTTCGGTCTTTTTATTGGACATTAAGATGAGGTGCCAAGGCATTGATAATTGCCGACTCATGCCTTTTAGTTTGATATATTTCGCTCTCAAAATTTATTATTTTTGGCTAATATAGGGATGGTGAGTATAGACCTAATGAATACGTATGGGTTGTGAGTTCAAGTACAGCTACCGTCTACTTATAGTATTCTCATTATGTGTTTTATTTTATTTTACTGGTTAAAATACCATTTTCGTCCTTGTAATTTGGAACTCATTCTATTTTAGTCTGCGTGCTTTTAAATGTCAAAAATTAGTCTCCGTACAATAAATCTTAAATTTTGTTTCTTATGAAGTTAATTTTTATTGAGTTCAGATAAATAATAATAATAATATTTTTTATTTAAGAAAGGATACAATGTGAATATCTTTCCCTAGTTTATAGATGGAAGACTAATAGTGACTAATTTCTTTTTAAGAAAAGTTAACCATAAACTAATTATAGGGGCTAACTTTAATATTTATTGAATGTGAGACTAATTTTGGACCCTTGGAACTTGAAGTTACATGGATTGAAATAAAACAAGTTTCAAATTAGGACTGAAATGGTATCTAATCCGTTTTCAGTGTCGTGTTACAGAGTCAATAGCTTTTTTTGTAAGATTAGCCAAGTTGCATTTAAACAACCTTAACCTAATGCATACATATGGTTGCATTTTAAATCTACTTTTCCTCGTCACATCTTGATTTTCATCCAAGTAAACTGCTTGTGAATGATTTTCGGCTTCATGGTCATACATCTGCCAGTATTGTAGACTACTCTTGACCTTCATTGCTTTTCACTGCTTGTTTAGTCACCTCTTTCTTTTTCTGCTGCACCCAGATTTAGTCCTAAAGACTGGTATAACCATACCGGCCGGAACTTTAGTTGTTGTACCCTTAAAATTGGTACAAATGGATAGTTCAAGTTGGGGAAGTGATGCGGATAAGTTCAATCCCTACCGTTTTTTATCAGTGGCTTGTAATGGCGCTGCTACGAGTCAGCGAACATCACTTGCAGGTAAGTTACTACACGCTTAAGGATTTCATGCTTGCATAATCTACTACCTTACAACTCAGTCCACTTTTGCTTAGCTTCTTCCTTTGGAACTCTTCAAAATGTGGAACTCTTTTGTCTTTTCATTTGAAAGAGGTCTATGAAATTTTATTGGTTATTGTTCCAATTCCGTCACTGATATATTCGCATCTAAACACATTCAACATTGAGTTTAACTTATTGCACGACCGGGTGGGCTAATCAGTTTAGTGGGGAGCTGGCTATGTTTTGATTTTTGAGTAATATGACTCATGAGTGACTGTGAACAAGACTTCATTTTTTGGGACTCTATCAGAGTAGAGGATGTAATACAAAGTCTTTGATGTTCGTCTTTTTTGTTTATTGCCTTGTTTGTTTGTTTTTTTTTTTTTTGGGAATGCATTTCTAGAGTTCCATTTCTTTTAATTTTGGTCTACAAGTTCTATTTGGATTGGGTTTTTGTCCACTCAGACGCATAATTTTCCTTTATATTTAACCGCTTAAGAAAAAAGCATACAAAAGAAAAATGCAATTTGAATTTTTTATGAAGGTTGTGGTGTCAGCACAATTTATTGCAATAAAATGGTCTAGATTGCGTCTTAATGTGTCGTGATGCAGGAGAAAATGCTGGGGACGAAGGAGAGAGCTCCTTTGTTTTGAACGATCCAACCGGCAATGTTGCCTTTCTTCCCTTCGGCTTTGGTGCACGTTCCTGTGTTGGTCGGAAATTTATCCTACAGGGAGTAGCAACACTATTTGCCTCTTTGCTCGCCAACTACGAGGTTGGTAGTTTTTTTATTATTATTATTATTATTATATATATATATATATTAAACTGTTGTAAAGCAAATATCCTACAACACAAAAATTCTTGCTTCTCAACAGTGAATAAAAAATAGATAGATTGAGTTAGGCGGTTAAAAAGACCATGTAAATAATAAAAGGCTTAGATGAATTCATCACGTGGCCATTTACCTAGAATTTAATATCCTATATGTCACTTTGACAACCAAATGTAGAAGGTTCAGGTAGTTGTGTTAATAAAGGTGCGTGCAAGTTGGCTCGGACACTCGTAGATATCAACAAAAAAAGAAAAAAAAAAAAGAAAAACTAACATGTTATTGTTAACAATGAAGATAGAGTAAATTGATTGAGTTCTACTTTCATGTCAATTCTCCTTGTCCTCATCTTTTGTTTTGGGAAATTCACCAAGTTGTGCTCTCTTTTCCAGTACATTTTAGAATATATTAAACGTTTGGATTCACCTTGGTCTAAAAACTTAAGCTGTGTTTTTGGAACTGTGGCTTTCGAAGTAGTTGGTGACGCCCTGAATCTTGAGCCATTTTTAGGAGTCATTGAATGGTTTCATTGATTTTTGCCCTTTTCATCACAAATTGTAGATAAAACTTCAGTCAGAATCCAAGAATGTCTCAAAGCTGTCATCAAATACCTCTGCATCGCAAATGCTTCCGAATTCGAAGATAGTCTTCGCAAGGAGGAACAGCTAA

mRNA sequence

AAAAAAGAAAGCGCTGAAGTTCTTACAGTTTAGTTCGCTTTCCTTTCCACCGATGAGAAAATGAGCTCGAAATGCGACGGCACTACTCCTCTCAACGTCATCAAAATACTTCTCCGATCGGAATCTTCCCAGAACTGTATATCCGAAGAGAAGTCCCGGATTCTTGGGGATTTCGTCACTCGCGAAGTCAATGCGTTCCTCTGGTTTTCTCTCATCGCCATCACTGTGGTTCTGATCGGCAAGGTTGTTGAACTCTTCAGATTGTGGTCGAAGGCGAAGCAACTTCCCGGACCTCCTAGTGCGTCTTTCTGCGGTTACTCCAAGGTCATTTCGCGCCGGAATCTCACGGATCTATTATATGACTCACATAAAAAATATGGACCAGTCGTCAAGCTATGGTTGGGTCCCATGCAGCTTTTGGTGTCTGTAAAGGAGCCAGCTCTTCTCAAAGAAATTCTGGTAAAGGCCGAGGATAAATTGCTTTTGACAGGAAGGGCATTCAGATTGGCATTTGGGCGTTCAAGCCTCTTTACTTCCTCTTTAGAGAAGGTGCAAAACAGAAGACGGTGGCTAGCAGAAAAGTTAGATGAAATATTGTTCCAGAGAGCTAATGTTTTTCCTGCAAAGGCTGTGGATTGTTCTGTAGGGAGAGTGCAAGATATTATGATTGAAGAAAGTGTAGATTGTAATAAGGTTTCTCAACATTTGGCTTTTACATTGTTAGGGTGCACACTTTTTGGGGATGCTTTTTTGGGTTGGTCCAAGGCAACCATCTATGAGGAACTTCTGATGATGGTTGCAAAAGATGCCAGCTTTTGGGCCTCCTACAGTGTTACTCCTTTCTGGAAACAAGGATTCTGGCGGTACCAGCGCTTGTGCATGAAATTGAAATGCTTAACACAAGATATTGTTCAGCAATGTAGAAAACATTGCAAGCTCTCCTCTCACTCCCAGAATCAGAACCTTCAAAATGAAATCAAAAGTACTGGTGTGGAGGCTGAATTTGTCATACCACGGTGTCCTGCTACGGAGATGCGTAATAGCTGTTTCTTTTATGGTCTTCATGATCAGGTTAATCCTAATGAAGAACCAATTGGAAATATTATGGGTGTGATGTTTCATGGATGTCTAACTACCGCAAATTTGATTGCTTCCATTTTGGGAAGGCTTGCCACAAATCCTGAAATACAAGAAAAGATCAACTCAGAACTAAATATAGTACAACAGGGCTCGGTGAAAGATCACCAGAAAAATGTTAATAACATGCCACTTCTGTTGGCAACTATATATGAATCTGCTCGTCTTCTGCCAGCAGGGCCTCTGTTACAAAGATGTTCACTCAAACAAGATTTAGTCCTAAAGACTGGTATAACCATACCGGCCGGAACTTTAGTTGTTGTACCCTTAAAATTGGTACAAATGGATAGTTCAAGTTGGGGAAGTGATGCGGATAAGTTCAATCCCTACCGTTTTTTATCAGTGGCTTGTAATGGCGCTGCTACGAGTCAGCGAACATCACTTGCAGGAGAAAATGCTGGGGACGAAGGAGAGAGCTCCTTTGTTTTGAACGATCCAACCGGCAATGTTGCCTTTCTTCCCTTCGGCTTTGGTGCACGTTCCTGTGTTGGTCGGAAATTTATCCTACAGGGAGTAGCAACACTATTTGCCTCTTTGCTCGCCAACTACGAGATAAAACTTCAGTCAGAATCCAAGAATGTCTCAAAGCTGTCATCAAATACCTCTGCATCGCAAATGCTTCCGAATTCGAAGATAGTCTTCGCAAGGAGGAACAGCTAA

Coding sequence (CDS)

ATGAGCTCGAAATGCGACGGCACTACTCCTCTCAACGTCATCAAAATACTTCTCCGATCGGAATCTTCCCAGAACTGTATATCCGAAGAGAAGTCCCGGATTCTTGGGGATTTCGTCACTCGCGAAGTCAATGCGTTCCTCTGGTTTTCTCTCATCGCCATCACTGTGGTTCTGATCGGCAAGGTTGTTGAACTCTTCAGATTGTGGTCGAAGGCGAAGCAACTTCCCGGACCTCCTAGTGCGTCTTTCTGCGGTTACTCCAAGGTCATTTCGCGCCGGAATCTCACGGATCTATTATATGACTCACATAAAAAATATGGACCAGTCGTCAAGCTATGGTTGGGTCCCATGCAGCTTTTGGTGTCTGTAAAGGAGCCAGCTCTTCTCAAAGAAATTCTGGTAAAGGCCGAGGATAAATTGCTTTTGACAGGAAGGGCATTCAGATTGGCATTTGGGCGTTCAAGCCTCTTTACTTCCTCTTTAGAGAAGGTGCAAAACAGAAGACGGTGGCTAGCAGAAAAGTTAGATGAAATATTGTTCCAGAGAGCTAATGTTTTTCCTGCAAAGGCTGTGGATTGTTCTGTAGGGAGAGTGCAAGATATTATGATTGAAGAAAGTGTAGATTGTAATAAGGTTTCTCAACATTTGGCTTTTACATTGTTAGGGTGCACACTTTTTGGGGATGCTTTTTTGGGTTGGTCCAAGGCAACCATCTATGAGGAACTTCTGATGATGGTTGCAAAAGATGCCAGCTTTTGGGCCTCCTACAGTGTTACTCCTTTCTGGAAACAAGGATTCTGGCGGTACCAGCGCTTGTGCATGAAATTGAAATGCTTAACACAAGATATTGTTCAGCAATGTAGAAAACATTGCAAGCTCTCCTCTCACTCCCAGAATCAGAACCTTCAAAATGAAATCAAAAGTACTGGTGTGGAGGCTGAATTTGTCATACCACGGTGTCCTGCTACGGAGATGCGTAATAGCTGTTTCTTTTATGGTCTTCATGATCAGGTTAATCCTAATGAAGAACCAATTGGAAATATTATGGGTGTGATGTTTCATGGATGTCTAACTACCGCAAATTTGATTGCTTCCATTTTGGGAAGGCTTGCCACAAATCCTGAAATACAAGAAAAGATCAACTCAGAACTAAATATAGTACAACAGGGCTCGGTGAAAGATCACCAGAAAAATGTTAATAACATGCCACTTCTGTTGGCAACTATATATGAATCTGCTCGTCTTCTGCCAGCAGGGCCTCTGTTACAAAGATGTTCACTCAAACAAGATTTAGTCCTAAAGACTGGTATAACCATACCGGCCGGAACTTTAGTTGTTGTACCCTTAAAATTGGTACAAATGGATAGTTCAAGTTGGGGAAGTGATGCGGATAAGTTCAATCCCTACCGTTTTTTATCAGTGGCTTGTAATGGCGCTGCTACGAGTCAGCGAACATCACTTGCAGGAGAAAATGCTGGGGACGAAGGAGAGAGCTCCTTTGTTTTGAACGATCCAACCGGCAATGTTGCCTTTCTTCCCTTCGGCTTTGGTGCACGTTCCTGTGTTGGTCGGAAATTTATCCTACAGGGAGTAGCAACACTATTTGCCTCTTTGCTCGCCAACTACGAGATAAAACTTCAGTCAGAATCCAAGAATGTCTCAAAGCTGTCATCAAATACCTCTGCATCGCAAATGCTTCCGAATTCGAAGATAGTCTTCGCAAGGAGGAACAGCTAA

Protein sequence

MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIGKVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLLVSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILFQRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYEELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQNLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGPLLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLANYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS
Homology
BLAST of Carg02017 vs. NCBI nr
Match: KAG7031903.1 (Cytochrome P450 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60
           MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG
Sbjct: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60

Query: 61  KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120
           KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL
Sbjct: 61  KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120

Query: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180
           VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF
Sbjct: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180

Query: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240
           QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE
Sbjct: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240

Query: 241 ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300
           ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ
Sbjct: 241 ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300

Query: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360
           NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA
Sbjct: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360

Query: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420
           NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP
Sbjct: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420

Query: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480
           LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA
Sbjct: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480

Query: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540
           TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA
Sbjct: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540

Query: 541 NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS 579
           NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS
Sbjct: 541 NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS 578

BLAST of Carg02017 vs. NCBI nr
Match: KAG6601101.1 (Cytochrome P450 714C3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 572/578 (98.96%), Postives = 573/578 (99.13%), Query Frame = 0

Query: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60
           MSSKCDGTTPLNVIKILLRSESSQNCISEEKS ILGDFVTREVNAFLWFSLIAITVVLIG
Sbjct: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSGILGDFVTREVNAFLWFSLIAITVVLIG 60

Query: 61  KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120
           KVVELFRLWSKAKQLPGPPS SFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL
Sbjct: 61  KVVELFRLWSKAKQLPGPPSPSFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120

Query: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180
           VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF
Sbjct: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180

Query: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240
           QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE
Sbjct: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240

Query: 241 ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300
           ELLMMVAKDASFWASY VTPFWKQGFWRYQ LCMKLKCLTQDIVQQ RKHCKLSSHSQNQ
Sbjct: 241 ELLMMVAKDASFWASYRVTPFWKQGFWRYQLLCMKLKCLTQDIVQQYRKHCKLSSHSQNQ 300

Query: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360
           NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA
Sbjct: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360

Query: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420
           NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP
Sbjct: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420

Query: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480
           LLQRCSL+QDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA
Sbjct: 421 LLQRCSLQQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480

Query: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540
           TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA
Sbjct: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540

Query: 541 NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS 579
           NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS
Sbjct: 541 NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS 578

BLAST of Carg02017 vs. NCBI nr
Match: XP_022957389.1 (cytochrome P450 714C3-like [Cucurbita moschata])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 568/578 (98.27%), Postives = 572/578 (98.96%), Query Frame = 0

Query: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60
           MSSKCDGTTPLNVI+ILLRSESSQNCISEEKSRILGDFVTREVN FLWFSLIAITVVLIG
Sbjct: 1   MSSKCDGTTPLNVIEILLRSESSQNCISEEKSRILGDFVTREVNVFLWFSLIAITVVLIG 60

Query: 61  KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120
           KVVELFRLWSKAKQLPGPPS SFCG+S VISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL
Sbjct: 61  KVVELFRLWSKAKQLPGPPSPSFCGHSNVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120

Query: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180
           VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF
Sbjct: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180

Query: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240
           QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE
Sbjct: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240

Query: 241 ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300
           ELLMMVAKDASFWASY VTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ
Sbjct: 241 ELLMMVAKDASFWASYRVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300

Query: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360
           NLQNEIKSTG+EAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA
Sbjct: 301 NLQNEIKSTGLEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360

Query: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420
           NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNV+NMPLLLATIYESARLLPAGP
Sbjct: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVDNMPLLLATIYESARLLPAGP 420

Query: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480
           LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA
Sbjct: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480

Query: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540
           TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA
Sbjct: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540

Query: 541 NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS 579
           NYEIKLQS SKN SKLSSNTSASQMLPNSKIVFARRNS
Sbjct: 541 NYEIKLQSGSKNDSKLSSNTSASQMLPNSKIVFARRNS 578

BLAST of Carg02017 vs. NCBI nr
Match: XP_022988815.1 (cytochrome P450 4C1-like [Cucurbita maxima])

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 558/578 (96.54%), Postives = 567/578 (98.10%), Query Frame = 0

Query: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60
           MSSKCDGTTPLN+I IL RSESS NCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG
Sbjct: 1   MSSKCDGTTPLNIIVILFRSESSHNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60

Query: 61  KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120
           KVVELFRLWSKAKQLPGPPS SFCG+SK ISRRNLTDLLYDSHK+YGPVVKLWLGPMQLL
Sbjct: 61  KVVELFRLWSKAKQLPGPPSPSFCGHSKAISRRNLTDLLYDSHKRYGPVVKLWLGPMQLL 120

Query: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180
           VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSS EKVQNRRRWLAEKLDEILF
Sbjct: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSFEKVQNRRRWLAEKLDEILF 180

Query: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240
           QRA+VFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE
Sbjct: 181 QRADVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240

Query: 241 ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300
           ELLMMVAKDASFWASY V PFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSS SQNQ
Sbjct: 241 ELLMMVAKDASFWASYRVAPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSRSQNQ 300

Query: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360
           NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA
Sbjct: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360

Query: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420
           NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKN++NMPLLLATIYESARLLPAGP
Sbjct: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNIDNMPLLLATIYESARLLPAGP 420

Query: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480
           LLQRCSLKQDLVL+TGITIPAGTLVVVPLKLVQM+SSSWGSDADKFNPYRFLSVACNGA 
Sbjct: 421 LLQRCSLKQDLVLQTGITIPAGTLVVVPLKLVQMESSSWGSDADKFNPYRFLSVACNGAD 480

Query: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540
           TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFI+QGVATLFASLLA
Sbjct: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFIIQGVATLFASLLA 540

Query: 541 NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS 579
           NYEIKLQSESKN SKLSSNTSASQMLPNSKIVFARRNS
Sbjct: 541 NYEIKLQSESKNDSKLSSNTSASQMLPNSKIVFARRNS 578

BLAST of Carg02017 vs. NCBI nr
Match: XP_023514775.1 (cytochrome P450 714C3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 557/578 (96.37%), Postives = 564/578 (97.58%), Query Frame = 0

Query: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60
           MSSKCDGTT L   +ILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG
Sbjct: 1   MSSKCDGTTHL---EILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60

Query: 61  KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120
           KVVELFRLWSKAKQLPGPPS SFCG+SKVISRRNLTD+LYDSHKKYGPVVKLWLGPMQLL
Sbjct: 61  KVVELFRLWSKAKQLPGPPSPSFCGHSKVISRRNLTDVLYDSHKKYGPVVKLWLGPMQLL 120

Query: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180
           VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSS EKVQN+RRWLAEKLDEILF
Sbjct: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSFEKVQNKRRWLAEKLDEILF 180

Query: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240
           QRANVFPAKAVD SVGRVQDIMIEE VDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE
Sbjct: 181 QRANVFPAKAVDYSVGRVQDIMIEEGVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240

Query: 241 ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300
           ELLMMVAKDASFWASY V PFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ
Sbjct: 241 ELLMMVAKDASFWASYRVAPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300

Query: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360
           NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA
Sbjct: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360

Query: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420
           NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNV+NMPLLLATIYESARLLPAGP
Sbjct: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVDNMPLLLATIYESARLLPAGP 420

Query: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480
           LLQRCSLKQDLVLKTGITIP GTLVVVPLKLVQMD+SSWGSDADKFNPYRFLSVACNGA 
Sbjct: 421 LLQRCSLKQDLVLKTGITIPTGTLVVVPLKLVQMDNSSWGSDADKFNPYRFLSVACNGAD 480

Query: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540
            SQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFI+QGVATLFASLLA
Sbjct: 481 MSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFIIQGVATLFASLLA 540

Query: 541 NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS 579
           NYEIKLQSESKN SKLSSNTSASQMLPNSKIVFARRNS
Sbjct: 541 NYEIKLQSESKNDSKLSSNTSASQMLPNSKIVFARRNS 575

BLAST of Carg02017 vs. ExPASy Swiss-Prot
Match: O70537 (Cytochrome P450 3A31 OS=Mesocricetus auratus OX=10036 GN=CYP3A31 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 6.9e-14
Identity = 61/206 (29.61%), Postives = 99/206 (48.06%), Query Frame = 0

Query: 352 MFHGCLTTANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYE 411
           +F G  TT++ ++  L  LAT+P++Q+K+  E++I      +     V  M  L   + E
Sbjct: 301 IFAGYDTTSSTLSFALYLLATHPDVQKKLQEEIDIALPNKARPSYDKVMEMEYLDMVLNE 360

Query: 412 SARLLPAGPLLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRF 471
           + RL P G  L+R   KQD+ +  G+ +P G++V+VP+  +  D   W  + +KF P RF
Sbjct: 361 TLRLYPIGSRLERV-CKQDVEM-DGVFVPKGSIVMVPVFALHYDPQYW-PEPEKFRPERF 420

Query: 472 LSVACNGAATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGV 531
                           + EN G          DP     FLPFG G R+C+G +F L  +
Sbjct: 421 ----------------SKENKGS--------IDP---YIFLPFGNGPRNCIGMRFALMNM 476

Query: 532 ATLFASLLANYEIKLQSESKNVSKLS 558
                 +L N+ ++   E++   KLS
Sbjct: 481 KLALTKVLQNFSLQPCKETQIPMKLS 476

BLAST of Carg02017 vs. ExPASy Swiss-Prot
Match: Q9T0K2 (Cytochrome P450 71A20 OS=Arabidopsis thaliana OX=3702 GN=CYP71A20 PE=2 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 1.5e-13
Identity = 57/206 (27.67%), Postives = 91/206 (44.17%), Query Frame = 0

Query: 348 IMGVMFHGCLTTANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLA 407
           I  +   G  TT + +   +  L  NP++ +K+  E+       +   +K    M  L A
Sbjct: 294 IWDMFLAGTATTLSFLEWAMTELMRNPKVMKKLQEEIRSSSPQDLFVTEKEAEKMNYLQA 353

Query: 408 TIYESARLLPAGPLLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFN 467
            I E+ RL P  PLL    L +D+ LK G  IPAGT V+V    +Q D+++WG+DA++F 
Sbjct: 354 VIKEALRLRPPAPLLVPRVLSEDVKLK-GYNIPAGTQVIVNAWAIQRDTTTWGTDAEEFK 413

Query: 468 PYRFLSVACNGAATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFI 527
           P R L                  N   +G+          +  F+PFG G R C G  F 
Sbjct: 414 PERHLDT----------------NLDFQGQ----------DFKFIPFGSGKRICPGIGFT 472

Query: 528 LQGVATLFASLLANYEIKLQSESKNV 554
              +    A+++  +  ++  E + V
Sbjct: 474 SALIGVTLANIVKRFNWRMDVEPQRV 472

BLAST of Carg02017 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.6e-13
Identity = 61/212 (28.77%), Postives = 95/212 (44.81%), Query Frame = 0

Query: 342 EEPIGNIMGVMFHGCLTTANLIASILGRLATNPEIQEKINSEL-NIVQQGSVKDHQKNVN 401
           +E I       F G  TTANL+      L+ + + QEK+  E+ N   +  + D  +  +
Sbjct: 367 DEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPD-AETCS 426

Query: 402 NMPLLLATIYESARLLPAGPLLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWG 461
            + L+     ES RL   GP+L    L  + +    + IP GT +++P+  +  D + WG
Sbjct: 427 KLKLMNTVFMESLRLY--GPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWG 486

Query: 462 SDADKFNPYRFLSVACNGAATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARS 521
           SDADKFNP RF     NG                    S   N P    A L F  G R+
Sbjct: 487 SDADKFNPMRF----ANGL-------------------SRAANHPN---ALLAFSMGPRA 546

Query: 522 CVGRKFILQGVATLFASLLANYEIKLQSESKN 553
           C+G+ F +    T+ A +L  + + L ++ K+
Sbjct: 547 CIGQNFAIMEAKTVLAMILQRFRLNLSADYKH 549

BLAST of Carg02017 vs. ExPASy Swiss-Prot
Match: W7LC91 (Cytochrome P450 monooxygenase FUM15 OS=Gibberella moniliformis (strain M3125 / FGSC 7600) OX=334819 GN=FUM15 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.6e-13
Identity = 72/258 (27.91%), Postives = 113/258 (43.80%), Query Frame = 0

Query: 327 NSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTANLIASILGRLATNPEIQEKINSELNI 386
           N      L + V  +E+ I  +M  +  G  TTA  +   +  L   PE+Q ++  E+ +
Sbjct: 325 NDILTVALLNGVFTDEQLIDQLMTFLAAGHETTATALTWAIYILCKQPEVQNRLREEIRM 384

Query: 387 ----------VQQGSVKDHQKNVN-NMPLLLATIYESARLLPAGPLLQRCSLKQDLVLKT 446
                      ++ S    Q+ ++  +P L     E  R     PL  R +     +L T
Sbjct: 385 HFPNPKGWPRSERPSSNTLQQAIDFKLPYLNVVCLEVMRYFAPIPLTMREATCDTTILHT 444

Query: 447 GITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVA-CNGAATSQRTSLAGENAGD 506
              +PAGT +++  ++   DS+ WG DA+ FNP R+LS    N  AT Q     G    D
Sbjct: 445 --FVPAGTRIILAPRVTNRDSALWGPDANNFNPDRWLSPKNGNREATEQSKFKIGNQKRD 504

Query: 507 EGESSFVLNDPT-------GNVAFLPFGFGARSCVGRKFILQGVATLFASLLANYEIKLQ 562
              +  V  +          N A L F  G RSC+G+ F     A L A+L+AN+E +++
Sbjct: 505 STAAPEVTQEEVRGRTEARSNYADLTFLHGPRSCIGQSFARVEFAILLATLIANFEFQIE 564

BLAST of Carg02017 vs. ExPASy Swiss-Prot
Match: O48921 (Cytochrome P450 97B2, chloroplastic OS=Glycine max OX=3847 GN=CYP97B2 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 4.5e-13
Identity = 64/222 (28.83%), Postives = 105/222 (47.30%), Query Frame = 0

Query: 347 NIMGVMFHGCLTTANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLL 406
           ++M ++  G  TTA ++   +  LA NP   +K  +E+++V  G+ +   +++  +  + 
Sbjct: 346 DLMTMLIAGHETTAAVLTWAVFLLAQNPSKMKKAQAEVDLV-LGTGRPTFESLKELQYIR 405

Query: 407 ATIYESARLLPAGPLLQRCSLKQDLV------LKTGITIPAGTLVVVPLKLVQMDSSSWG 466
             + E+ RL P  PLL R SLK D++       K G  IPAGT V + +  +      W 
Sbjct: 406 LIVVEALRLYPQPPLLIRRSLKSDVLPGGHKGEKDGYAIPAGTDVFISVYNLHRSPYFW- 465

Query: 467 SDADKFNPYRFLSVACNGAATSQRTSLAGENAGDEGESSFVL--NDPTGNVAFLPFGFGA 526
              D F P RFL         ++   + G    D   S   L  N+   + AFLPFG G 
Sbjct: 466 DRPDDFEPERFL-------VQNKNEEIEGWAGLDPSRSPGALYPNEVISDFAFLPFGGGP 525

Query: 527 RSCVGRKFILQGVATLFASLLANYEIKLQSESKNVSKLSSNT 561
           R CVG +F L         LL N++++L+   ++V  ++  T
Sbjct: 526 RKCVGDQFALMESTVALTMLLQNFDVELKGTPESVELVTGAT 558

BLAST of Carg02017 vs. ExPASy TrEMBL
Match: A0A6J1H1T3 (cytochrome P450 714C3-like OS=Cucurbita moschata OX=3662 GN=LOC111458801 PE=3 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 568/578 (98.27%), Postives = 572/578 (98.96%), Query Frame = 0

Query: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60
           MSSKCDGTTPLNVI+ILLRSESSQNCISEEKSRILGDFVTREVN FLWFSLIAITVVLIG
Sbjct: 1   MSSKCDGTTPLNVIEILLRSESSQNCISEEKSRILGDFVTREVNVFLWFSLIAITVVLIG 60

Query: 61  KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120
           KVVELFRLWSKAKQLPGPPS SFCG+S VISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL
Sbjct: 61  KVVELFRLWSKAKQLPGPPSPSFCGHSNVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120

Query: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180
           VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF
Sbjct: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180

Query: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240
           QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE
Sbjct: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240

Query: 241 ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300
           ELLMMVAKDASFWASY VTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ
Sbjct: 241 ELLMMVAKDASFWASYRVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300

Query: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360
           NLQNEIKSTG+EAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA
Sbjct: 301 NLQNEIKSTGLEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360

Query: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420
           NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNV+NMPLLLATIYESARLLPAGP
Sbjct: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVDNMPLLLATIYESARLLPAGP 420

Query: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480
           LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA
Sbjct: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480

Query: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540
           TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA
Sbjct: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540

Query: 541 NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS 579
           NYEIKLQS SKN SKLSSNTSASQMLPNSKIVFARRNS
Sbjct: 541 NYEIKLQSGSKNDSKLSSNTSASQMLPNSKIVFARRNS 578

BLAST of Carg02017 vs. ExPASy TrEMBL
Match: A0A6J1JKM0 (cytochrome P450 4C1-like OS=Cucurbita maxima OX=3661 GN=LOC111486044 PE=3 SV=1)

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 558/578 (96.54%), Postives = 567/578 (98.10%), Query Frame = 0

Query: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60
           MSSKCDGTTPLN+I IL RSESS NCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG
Sbjct: 1   MSSKCDGTTPLNIIVILFRSESSHNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60

Query: 61  KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120
           KVVELFRLWSKAKQLPGPPS SFCG+SK ISRRNLTDLLYDSHK+YGPVVKLWLGPMQLL
Sbjct: 61  KVVELFRLWSKAKQLPGPPSPSFCGHSKAISRRNLTDLLYDSHKRYGPVVKLWLGPMQLL 120

Query: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180
           VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSS EKVQNRRRWLAEKLDEILF
Sbjct: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSFEKVQNRRRWLAEKLDEILF 180

Query: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240
           QRA+VFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE
Sbjct: 181 QRADVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240

Query: 241 ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300
           ELLMMVAKDASFWASY V PFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSS SQNQ
Sbjct: 241 ELLMMVAKDASFWASYRVAPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSRSQNQ 300

Query: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360
           NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA
Sbjct: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360

Query: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420
           NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKN++NMPLLLATIYESARLLPAGP
Sbjct: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNIDNMPLLLATIYESARLLPAGP 420

Query: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480
           LLQRCSLKQDLVL+TGITIPAGTLVVVPLKLVQM+SSSWGSDADKFNPYRFLSVACNGA 
Sbjct: 421 LLQRCSLKQDLVLQTGITIPAGTLVVVPLKLVQMESSSWGSDADKFNPYRFLSVACNGAD 480

Query: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540
           TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFI+QGVATLFASLLA
Sbjct: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFIIQGVATLFASLLA 540

Query: 541 NYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRNS 579
           NYEIKLQSESKN SKLSSNTSASQMLPNSKIVFARRNS
Sbjct: 541 NYEIKLQSESKNDSKLSSNTSASQMLPNSKIVFARRNS 578

BLAST of Carg02017 vs. ExPASy TrEMBL
Match: A0A6J1G0Z6 (cytochrome P450 714C3-like OS=Cucurbita moschata OX=3662 GN=LOC111449685 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.5e-274
Identity = 483/578 (83.56%), Postives = 525/578 (90.83%), Query Frame = 0

Query: 1   MSSKCDG-TTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLI 60
           MSSKCDG TTP+NVI ILLR ESS+NC S++ SRIL DFVTREVNAFLWFS+IAIT VLI
Sbjct: 1   MSSKCDGTTTPVNVIGILLRLESSRNCNSDQNSRILRDFVTREVNAFLWFSVIAITAVLI 60

Query: 61  GKVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQL 120
            KVV LFRLWSKAK LPGPP  SF G+S+VISRRNLTDLLYDSHK+YGPVVKLWLGPMQL
Sbjct: 61  NKVVALFRLWSKAKLLPGPPCPSFYGHSEVISRRNLTDLLYDSHKQYGPVVKLWLGPMQL 120

Query: 121 LVSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEIL 180
           LVSVKEPAL+KEIL+KAEDKL LTGR FR AFGRSSLF SS EKVQNRR+ LAEKLD+I 
Sbjct: 121 LVSVKEPALIKEILLKAEDKLPLTGRVFRFAFGRSSLFASSFEKVQNRRQLLAEKLDQIS 180

Query: 181 FQRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIY 240
           F+R NV PAKAVDCSVGRVQD+MIEES+DCNKVSQHLAFTLLGCTLFGDAFLGWSKATIY
Sbjct: 181 FRRDNVVPAKAVDCSVGRVQDLMIEESIDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIY 240

Query: 241 EELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQN 300
           EELLMM+AKDASFWASY VTPFWKQGFWRYQRLC+KLKCLTQDIVQQ RK CK  SH  +
Sbjct: 241 EELLMMIAKDASFWASYRVTPFWKQGFWRYQRLCVKLKCLTQDIVQQYRKSCKPFSHCYS 300

Query: 301 QNLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTT 360
           QNL NEIKSTGVE  F IP CPA EMRN+ FFYGL+D  NPNEEP GNIMGVMFHGCLTT
Sbjct: 301 QNLHNEIKSTGVEVAFDIPSCPAAEMRNNGFFYGLNDHANPNEEPYGNIMGVMFHGCLTT 360

Query: 361 ANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAG 420
           A+LIASIL RL++NPEIQEKINSELN  ++GSVKD+QKNV+NMPLLLATIYESARLLPAG
Sbjct: 361 ASLIASILERLSSNPEIQEKINSELNSARKGSVKDNQKNVDNMPLLLATIYESARLLPAG 420

Query: 421 PLLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGA 480
           PLLQRCSLKQDLVLKTGITIPAGTLVVVP+KLVQMD++SWGS+ ++FNPYRFLS ACNG 
Sbjct: 421 PLLQRCSLKQDLVLKTGITIPAGTLVVVPVKLVQMDNTSWGSNVNEFNPYRFLSKACNGT 480

Query: 481 ATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLL 540
            T+QRTSLAGENA D+GESSFVLNDPTG + FLPFGFGAR+CVG+KFI+QGVATLFASLL
Sbjct: 481 DTTQRTSLAGENAEDQGESSFVLNDPTGKIEFLPFGFGARACVGQKFIIQGVATLFASLL 540

Query: 541 ANYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRN 578
           ANYEIKLQSESKN SKLS++ SASQ LPNSKIVF +RN
Sbjct: 541 ANYEIKLQSESKNGSKLSTSPSASQTLPNSKIVFVKRN 578

BLAST of Carg02017 vs. ExPASy TrEMBL
Match: A0A6J1HSZ9 (cytochrome P450 83A1-like OS=Cucurbita maxima OX=3661 GN=LOC111466505 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.1e-271
Identity = 479/578 (82.87%), Postives = 520/578 (89.97%), Query Frame = 0

Query: 1   MSSKCDG-TTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLI 60
           MSSKCDG TTP+NVI ILLR ESS+NC S++ SRIL DFVTREVNAFLWFS+IAIT VLI
Sbjct: 1   MSSKCDGTTTPVNVIGILLRLESSRNCNSDQNSRILRDFVTREVNAFLWFSVIAITAVLI 60

Query: 61  GKVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQL 120
            KVV LFRLWSKAK LPGPP  SF G+S+VISRRNLTDLLYDSHKKYGPVVKLWLGPMQL
Sbjct: 61  NKVVALFRLWSKAKLLPGPPCPSFYGHSEVISRRNLTDLLYDSHKKYGPVVKLWLGPMQL 120

Query: 121 LVSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEIL 180
           LVSVKEPAL+KEIL+KAEDKL LTGR FR AFGRSSLF SS EKVQNRR+ LAEKLD+I 
Sbjct: 121 LVSVKEPALIKEILLKAEDKLPLTGRVFRFAFGRSSLFASSFEKVQNRRQLLAEKLDQIS 180

Query: 181 FQRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIY 240
           F+RANV PAKAVD SVGRVQD+MIEES+DCNKVSQHLAFTLLGCTLFGDAFLGWSKATIY
Sbjct: 181 FRRANVIPAKAVDSSVGRVQDLMIEESIDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIY 240

Query: 241 EELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQN 300
           EELLMM+AKDASFWASY VTPFWKQ FWRYQRLC+KLKCLTQDIVQ+ RK CK  SH  +
Sbjct: 241 EELLMMIAKDASFWASYKVTPFWKQEFWRYQRLCVKLKCLTQDIVQKYRKSCKPFSHCYS 300

Query: 301 QNLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTT 360
           QNL NEIKSTG E  F IP CPA EMRN+ FFYGL D  NPNEEP GNIMGVMFHGCLTT
Sbjct: 301 QNLHNEIKSTGGEVAFDIPSCPAAEMRNNGFFYGLDDHANPNEEPYGNIMGVMFHGCLTT 360

Query: 361 ANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAG 420
           A+LIASIL RL++NPEIQEKINSELN  ++GSVKD+QKNV+NMPLLLATIYES RLLPAG
Sbjct: 361 ASLIASILERLSSNPEIQEKINSELNSARKGSVKDNQKNVDNMPLLLATIYESVRLLPAG 420

Query: 421 PLLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGA 480
           PLLQRCSLKQDLVLKTGITIPAGTLVVVP+KLVQMD++SWGS+ ++FNPYRFLS ACNG 
Sbjct: 421 PLLQRCSLKQDLVLKTGITIPAGTLVVVPVKLVQMDNTSWGSNVNEFNPYRFLSKACNGT 480

Query: 481 ATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLL 540
            T+Q+ SLAGENA D+GESSFVLNDPTG + FLPFGFGAR+CVG+KFI+QGVATLFASLL
Sbjct: 481 DTTQQASLAGENAEDQGESSFVLNDPTGKIEFLPFGFGARACVGQKFIIQGVATLFASLL 540

Query: 541 ANYEIKLQSESKNVSKLSSNTSASQMLPNSKIVFARRN 578
           ANYEIKLQSESKN SKLS+N SASQ LPNSKIVF +RN
Sbjct: 541 ANYEIKLQSESKNGSKLSTNPSASQTLPNSKIVFVKRN 578

BLAST of Carg02017 vs. ExPASy TrEMBL
Match: A0A5A7SU18 (2-hydroxyisoflavanone synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002950 PE=3 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 3.0e-270
Identity = 479/580 (82.59%), Postives = 516/580 (88.97%), Query Frame = 0

Query: 1   MSSKCDGTTPLNVIKILLRSESSQNCISEEKSRILGDFVTREVNAFLWFSLIAITVVLIG 60
           MSS+CDG TP NVI ILLR ESS+NC SE+KSRIL DFVTREVNAFLWF LIAIT VLI 
Sbjct: 1   MSSECDGFTPFNVIGILLRLESSRNCNSEQKSRILRDFVTREVNAFLWFFLIAITAVLIS 60

Query: 61  KVVELFRLWSKAKQLPGPPSASFCGYSKVISRRNLTDLLYDSHKKYGPVVKLWLGPMQLL 120
           KVV LF+LWSKAK LPGPP  SF G+SKVISRRNLTD+LYDSHKKYG V+KLWLGPMQLL
Sbjct: 61  KVVALFKLWSKAKLLPGPPCPSFYGHSKVISRRNLTDILYDSHKKYGSVIKLWLGPMQLL 120

Query: 121 VSVKEPALLKEILVKAEDKLLLTGRAFRLAFGRSSLFTSSLEKVQNRRRWLAEKLDEILF 180
           VSVKEPALLKEILVKAEDKL LTGRAFRLAFGRSSLF SS EKVQ+RR WLAEKLD I F
Sbjct: 121 VSVKEPALLKEILVKAEDKLPLTGRAFRLAFGRSSLFASSFEKVQSRRLWLAEKLDGISF 180

Query: 181 QRANVFPAKAVDCSVGRVQDIMIEESVDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240
           QRANV PAKAVDCSVGR+QD+M+EES+DCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE
Sbjct: 181 QRANVIPAKAVDCSVGRIQDLMVEESIDCNKVSQHLAFTLLGCTLFGDAFLGWSKATIYE 240

Query: 241 ELLMMVAKDASFWASYSVTPFWKQGFWRYQRLCMKLKCLTQDIVQQCRKHCKLSSHSQNQ 300
           ELLMM+AKDA+ WASY VTPFWKQGFWRYQRLCMKLKCLTQDIVQQ +KH  L SHS NQ
Sbjct: 241 ELLMMIAKDANIWASYRVTPFWKQGFWRYQRLCMKLKCLTQDIVQQYKKHYNLFSHSHNQ 300

Query: 301 NLQNEIKSTGVEAEFVIPRCPATEMRNSCFFYGLHDQVNPNEEPIGNIMGVMFHGCLTTA 360
            LQ+EIKS+GVE  F IP CP  EM NSCFF G +D VN NEEP GNIMGVMFHGCLTTA
Sbjct: 301 KLQSEIKSSGVEVAFDIPPCPIAEMCNSCFFSGPNDHVNSNEEPYGNIMGVMFHGCLTTA 360

Query: 361 NLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLATIYESARLLPAGP 420
           NLIASIL RLATNPEIQEKINSELN   + SVKD Q NV+NMPLLLATIYESARLLP+GP
Sbjct: 361 NLIASILERLATNPEIQEKINSELNRALKDSVKDPQNNVDNMPLLLATIYESARLLPSGP 420

Query: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFNPYRFLSVACNGAA 480
           LLQRCSLKQDLVLKTGITIPAGTLVVVP+KL+QMDSSSWGSDA++F+PYRFLS+ACNG  
Sbjct: 421 LLQRCSLKQDLVLKTGITIPAGTLVVVPIKLIQMDSSSWGSDANEFSPYRFLSMACNGTD 480

Query: 481 TSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFILQGVATLFASLLA 540
             QRTS+AGEN GDEG+SSFVLNDPTGNVAFLPFGFGARSCVG+K I+QG+ATLFASLL+
Sbjct: 481 MRQRTSVAGENTGDEGDSSFVLNDPTGNVAFLPFGFGARSCVGQKIIIQGLATLFASLLS 540

Query: 541 NYEIKLQSESKNV--SKLSSNTSASQMLPNSKIVFARRNS 579
           NYEIKLQSESK+   SK SSN S +Q+L NSK+ F RRNS
Sbjct: 541 NYEIKLQSESKSKTDSKSSSNPSTAQILLNSKMAFIRRNS 580

BLAST of Carg02017 vs. TAIR 10
Match: AT4G13310.1 (cytochrome P450, family 71, subfamily A, polypeptide 20 )

HSP 1 Score: 79.3 bits (194), Expect = 1.1e-14
Identity = 57/206 (27.67%), Postives = 91/206 (44.17%), Query Frame = 0

Query: 348 IMGVMFHGCLTTANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLA 407
           I  +   G  TT + +   +  L  NP++ +K+  E+       +   +K    M  L A
Sbjct: 294 IWDMFLAGTATTLSFLEWAMTELMRNPKVMKKLQEEIRSSSPQDLFVTEKEAEKMNYLQA 353

Query: 408 TIYESARLLPAGPLLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFN 467
            I E+ RL P  PLL    L +D+ LK G  IPAGT V+V    +Q D+++WG+DA++F 
Sbjct: 354 VIKEALRLRPPAPLLVPRVLSEDVKLK-GYNIPAGTQVIVNAWAIQRDTTTWGTDAEEFK 413

Query: 468 PYRFLSVACNGAATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFI 527
           P R L                  N   +G+          +  F+PFG G R C G  F 
Sbjct: 414 PERHLDT----------------NLDFQGQ----------DFKFIPFGSGKRICPGIGFT 472

Query: 528 LQGVATLFASLLANYEIKLQSESKNV 554
              +    A+++  +  ++  E + V
Sbjct: 474 SALIGVTLANIVKRFNWRMDVEPQRV 472

BLAST of Carg02017 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 78.6 bits (192), Expect = 1.9e-14
Identity = 61/212 (28.77%), Postives = 95/212 (44.81%), Query Frame = 0

Query: 342 EEPIGNIMGVMFHGCLTTANLIASILGRLATNPEIQEKINSEL-NIVQQGSVKDHQKNVN 401
           +E I       F G  TTANL+      L+ + + QEK+  E+ N   +  + D  +  +
Sbjct: 367 DEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPD-AETCS 426

Query: 402 NMPLLLATIYESARLLPAGPLLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWG 461
            + L+     ES RL   GP+L    L  + +    + IP GT +++P+  +  D + WG
Sbjct: 427 KLKLMNTVFMESLRLY--GPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWG 486

Query: 462 SDADKFNPYRFLSVACNGAATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARS 521
           SDADKFNP RF     NG                    S   N P    A L F  G R+
Sbjct: 487 SDADKFNPMRF----ANGL-------------------SRAANHPN---ALLAFSMGPRA 546

Query: 522 CVGRKFILQGVATLFASLLANYEIKLQSESKN 553
           C+G+ F +    T+ A +L  + + L ++ K+
Sbjct: 547 CIGQNFAIMEAKTVLAMILQRFRLNLSADYKH 549

BLAST of Carg02017 vs. TAIR 10
Match: AT4G13290.1 (cytochrome P450, family 71, subfamily A, polypeptide 19 )

HSP 1 Score: 76.3 bits (186), Expect = 9.3e-14
Identity = 52/206 (25.24%), Postives = 89/206 (43.20%), Query Frame = 0

Query: 348 IMGVMFHGCLTTANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNNMPLLLA 407
           I  +   G  T+ + +   +  L  NP++ +K+  E+    +  +   +K    M  L A
Sbjct: 287 IWDMFLAGTATSLSFLEWAMTELMRNPKVMKKLQEEIRSSSRQGLFVTEKEAEKMDYLQA 346

Query: 408 TIYESARLLPAGPLLQRCSLKQDLVLKTGITIPAGTLVVVPLKLVQMDSSSWGSDADKFN 467
            I E+ RL P  PL+      +D+ LK G  IPAGT V++    +Q D+++WG DA++F 
Sbjct: 347 VIKEALRLRPPAPLMVPRVFSEDVTLK-GYNIPAGTQVIINAWAIQRDTTTWGIDAEEFR 406

Query: 468 PYRFLSVACNGAATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGARSCVGRKFI 527
           P R L                            +L+    +  F+PFG G R C G  F 
Sbjct: 407 PERHL--------------------------DSILDFQGQDFKFIPFGSGKRICPGIGFT 465

Query: 528 LQGVATLFASLLANYEIKLQSESKNV 554
              +    A+++  +  ++  E + V
Sbjct: 467 SALIGVTLANIVKRFNWRMDVEPQRV 465

BLAST of Carg02017 vs. TAIR 10
Match: AT2G46960.1 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 73.9 bits (180), Expect = 4.6e-13
Identity = 66/214 (30.84%), Postives = 93/214 (43.46%), Query Frame = 0

Query: 342 EEPIGNIMGVMFHGCLTTANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNN 401
           EE I       F G  TT+NL+A     L+ + + QEK+  E+            +  + 
Sbjct: 197 EEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFSK 256

Query: 402 MPLLLATIYESARLL-PAGPLLQRCSLKQDLVLKTG-ITIPAGTLVVVPLKLVQMDSSSW 461
           + L+   I ES RL  P   L +  S+     +K G + IP GT VV+PL  +  D + W
Sbjct: 257 LKLMNMVIMESLRLYGPVSALAREASVN----IKLGDLEIPKGTTVVIPLLKMHSDKTLW 316

Query: 462 GSDADKFNPYRFLSVACNGAATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGAR 521
           GSDADKFNP RF     NG                    S   N P    A L F  G R
Sbjct: 317 GSDADKFNPMRF----ANGV-------------------SRAANHPN---ALLAFSVGPR 376

Query: 522 SCVGRKFILQGVATLFASLLANYE-IKLQSESKN 553
           +C+G+ F++    T+   +L  +  I L  E K+
Sbjct: 377 ACIGQNFVMIEAKTVLTMILQRFRFISLCDEYKH 380

BLAST of Carg02017 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 73.9 bits (180), Expect = 4.6e-13
Identity = 66/214 (30.84%), Postives = 93/214 (43.46%), Query Frame = 0

Query: 342 EEPIGNIMGVMFHGCLTTANLIASILGRLATNPEIQEKINSELNIVQQGSVKDHQKNVNN 401
           EE I       F G  TT+NL+A     L+ + + QEK+  E+            +  + 
Sbjct: 313 EEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFSK 372

Query: 402 MPLLLATIYESARLL-PAGPLLQRCSLKQDLVLKTG-ITIPAGTLVVVPLKLVQMDSSSW 461
           + L+   I ES RL  P   L +  S+     +K G + IP GT VV+PL  +  D + W
Sbjct: 373 LKLMNMVIMESLRLYGPVSALAREASVN----IKLGDLEIPKGTTVVIPLLKMHSDKTLW 432

Query: 462 GSDADKFNPYRFLSVACNGAATSQRTSLAGENAGDEGESSFVLNDPTGNVAFLPFGFGAR 521
           GSDADKFNP RF     NG                    S   N P    A L F  G R
Sbjct: 433 GSDADKFNPMRF----ANGV-------------------SRAANHPN---ALLAFSVGPR 492

Query: 522 SCVGRKFILQGVATLFASLLANYE-IKLQSESKN 553
           +C+G+ F++    T+   +L  +  I L  E K+
Sbjct: 493 ACIGQNFVMIEAKTVLTMILQRFRFISLCDEYKH 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031903.10.0e+00100.00Cytochrome P450 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6601101.10.0e+0098.96Cytochrome P450 714C3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022957389.10.0e+0098.27cytochrome P450 714C3-like [Cucurbita moschata][more]
XP_022988815.10.0e+0096.54cytochrome P450 4C1-like [Cucurbita maxima][more]
XP_023514775.10.0e+0096.37cytochrome P450 714C3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O705376.9e-1429.61Cytochrome P450 3A31 OS=Mesocricetus auratus OX=10036 GN=CYP3A31 PE=2 SV=1[more]
Q9T0K21.5e-1327.67Cytochrome P450 71A20 OS=Arabidopsis thaliana OX=3702 GN=CYP71A20 PE=2 SV=2[more]
F4IK452.6e-1328.77Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1[more]
W7LC912.6e-1327.91Cytochrome P450 monooxygenase FUM15 OS=Gibberella moniliformis (strain M3125 / F... [more]
O489214.5e-1328.83Cytochrome P450 97B2, chloroplastic OS=Glycine max OX=3847 GN=CYP97B2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H1T30.0e+0098.27cytochrome P450 714C3-like OS=Cucurbita moschata OX=3662 GN=LOC111458801 PE=3 SV... [more]
A0A6J1JKM00.0e+0096.54cytochrome P450 4C1-like OS=Cucurbita maxima OX=3661 GN=LOC111486044 PE=3 SV=1[more]
A0A6J1G0Z61.5e-27483.56cytochrome P450 714C3-like OS=Cucurbita moschata OX=3662 GN=LOC111449685 PE=3 SV... [more]
A0A6J1HSZ92.1e-27182.87cytochrome P450 83A1-like OS=Cucurbita maxima OX=3661 GN=LOC111466505 PE=3 SV=1[more]
A0A5A7SU183.0e-27082.592-hydroxyisoflavanone synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
AT4G13310.11.1e-1427.67cytochrome P450, family 71, subfamily A, polypeptide 20 [more]
AT2G46950.11.9e-1428.77cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT4G13290.19.3e-1425.24cytochrome P450, family 71, subfamily A, polypeptide 19 [more]
AT2G46960.14.6e-1330.84cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
AT2G46960.24.6e-1330.84cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 539..559
NoneNo IPR availablePANTHERPTHR24291:SF140CYTOCHROME P450 4C3-RELATEDcoord: 48..557
NoneNo IPR availablePANTHERPTHR24291CYTOCHROME P450 FAMILY 4coord: 48..557
IPR001128Cytochrome P450PRINTSPR00385P450coord: 407..418
score: 37.62
coord: 372..385
score: 35.04
coord: 521..532
score: 24.02
coord: 512..521
score: 59.48
IPR001128Cytochrome P450PFAMPF00067p450coord: 76..559
e-value: 7.7E-50
score: 170.0
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 363..389
score: 25.91
coord: 521..544
score: 21.92
coord: 103..122
score: 33.43
coord: 511..521
score: 56.54
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 67..572
e-value: 4.4E-64
score: 218.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 67..575
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 514..523

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02017-RACarg02017-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen