Carg01954 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01954
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionmRNA-decapping enzyme subunit 2-like
LocationCarg_Chr04: 8402044 .. 8405535 (-)
RNA-Seq ExpressionCarg01954
SyntenyCarg01954
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGCAACGCAGAAACTGACGACGTGTCGGCTCAGTTCTCTCAGTTCTTTAGGTAAAGGACGAGCAGAGTGTGAGCGGCAGATTCTTAATCAAAATCAACTAAGAAGCCATGGCCGGTATTCATCAGCCCGCTAATGCGTCGGTGAAGAACGGTCTTCCTCCCCAGGAGCTCCTTGATGATCTTTGCAGGTACTCACGCTTTGTTGTATTTCTCTCTATTTGATTTACTGGTTTTGCTGCGAGTTATTGGTTTGTTAGATGTGGAGATTTTAGGGTTCTTAGTTGAAATTAATGCGAGGTAGTTTACTTTGATTGTCATTATTTTTCATCCGTCAACTGGTTGCCATTTTCTAAAGCGAGTGATAATAATTCCTCTAAAAGTGATTGTCCTTGAACCTATTTTGGCGGCCTGTGTTCATTAAACCAATTGAACCAATAATTTAGGTCTAGTTTTGATAAACATTTATCAAAACAGAGAAATTCATAGATATAAGTAGTTTTTTGAGCTTGAGGCTACTGAACAAATGTTGTTCGTCTTGGTAGAACTAGTTTTTATGAACTTAATTTTCATAAACCCTAAAACAAGTGCTTATCAACACCTTAATTGTTGTTGTTTTTTTTTTTTTTTTTAACTGCATTATTGGTTGGAATAAACAAGTGTTTAGCTCTAAGATCTGTCATTCTCTTGTTGATTATGCAGCCGATTTGTTTTAAATGTGCCAAGGGAAGATCTACAATCATTTGAGAGGATTCTGTTTCTAGTTGAATATGCACATTGGTTCTATGAGGACAACTCGGTAGAGAATAACCCTTCACTGAAGTCCTTCAACTTGAAGGAGTTCACTTCGTTGCGTATCCTAAGTTTAGCGTAGTTTATGGCATCTCTGTCGGCTTTAACGCTGAAAGTATCTTAGAGAAAGAGCGCAAAATGCTTTTACTGTTTTTCCTGTATATATCGTTGTCTTCCTTAATTTTCTGTAGTATTTAACAGTTGTGACGTTTTGAAACCATATGTGCCCCACATAGATGACATATTTAAGGACTTCACTTCCTATAAGCTTCGAGTTCCTGTCACTGGGGGAATTATTTTGGATGAAACCTTTGAGAGGGTAAGTGTTGGATTGAAAAAGGGTTGTATTTATTTATTTATTTTTCCCTTAATACTGTGATTGATTTGGTTATAGTAATACTATTTTTGTGCTTCCTTTTGTTTCTGCAACCAAACAGTGCTTACTGGTGAAGGGATGGAAAGGGTCAAGCTGGAGTTTTCCTCGTGGAAAAAAGAGCAAGGATGAAGAAGATCATGCTTGTGCCATTAGAGAAGTAATCTTTTCTTATTTTTGAACTTTAATGACAATAATTTAGTATTTTTGTGTTTGAAAATGCCAACATTTACTGAGAAAAATGAAAGAATACGTAAGGATATTTCCAGAAAAGAAAAAACGAAAGAGTCTCCGGACTTGGAGTTAGAAGACCTTAATTAAGGAGGGCTTTAGTAAATTTGGAGTGTAACCTAGCTTGATACTTTCTTTGCTTCCGTCGCTCAGTACATCCCCACTACTGACCCTTACAAACTTTTTGTTTAACTGTCAAGATCTCTTGCTCGGTGTGCTTGTTACATGCTTAAATTTTTTCTCAGCGTTTCAACGAGAGGTACAAAACAGCTGTCAAAAAGGGTTGCCATTAGACAGAGGAAAAAAAAACACTAAAATTCTTTGTTACAATAATTCAAAGGGAATAAGGCTCGAACTTCCTCCAAGAACTCACCACGCCTCTTAAAAACTCTCCTATTTCTCTCCTGTTGAAGGAAAGGAGGGTGAAATAGCAGCTCCTGGACCCTAAATGATTATACCTCCCCCTATGTCACCGTCAATTTAAGCTTCATGGACTTGAGAACTGGTTATTTGCCATCTTATATTATTTGTTTCGAGCAAAAACGAGCATCCAAGAGGTTTAATAATTGTTTCAGGGCTTTAATGGATTATTTATGGAATATATTTTACGTTGAGATTGTAGGTTTTGGAGGAAACTGGTTTTGACGTGACACCGTTCCTCATCGAAGATGATTTTCTTGAAGTGATGTTTGGACAACAGAGAGTGCGGCTTTACATTATAGCTGGGGTGAAAACTGATATCCCCTTTGCACCTCTAACGAAGAAAGAGATCAGTGTATGTATTCCTTCTCCATTTTTCTTTCGTCTTCAAATTTGAAATTCTTTAATTTGGAATTTTGATTACATTTTTTACATTTAACGCTCGATTTGGCTCAAAACTATGATTTCTGCACTTTTCTACCTTATCGTTTATATTTACGACGTTTATCATTGATAATGGGGTGATTGGTATGCCTTTCAAGGCCTGAGTTGAAGTAATCAGTTCTTATAATGGACACGTTAAGAGTTAAGCTCATGCCCAAGTGCTTATTTTGCTTGATAGGACCTCATTCTTATGGATATGTTTTTTGTAAGCAGGAAATTGCATGGCATCGACTTGACGACCTTCTACCCGCAAGTGACGATGTGATATCTCATGGAATCACCGGCCTCAAGCTTTACATGGTGGCTCCTTTCTTGAAGTGAGCATCCTTGTGTAGTTTTCTTGATTCCCCCAGCAGGCAATTTTGAAATTAGTTTATGTTTAATGGAGTCGACAATTTTGAAATGCAGCTCGTTGAGATCATGGATCTTGAATCATCAGCCGCCTGTGAATCCGAACTTCGACATGCATGTAAAAGGTAATCCGTTCGTGACTTACAAGGTTCCGATACTAAACATCGATATTGCCATAGCCAACTCAATCCGTGTAACCAAACCTGTTTCCAATAATTTGCAATACACTTAGCTTACCGACTTTCTCGCATTCTTTCCCAGTGAGCGCCCTATTTCGTGATTATAATCTTTTGTACTTTTCATTCAGGAGTCACCATGTGGAAGGTGAAGAACAGCTCGATCGTTTTCGACAGCCAGCAAACAAAATTTGATTCTGATCCTGGACAAAGCCTCAGAAACTTCAAATTCGATACAGCCTCTATCTTGCAGGCTATGGAAAGTGCTTTTGCTGCTTGAGTTTCAACAAAAGCCTCGAACGCCTCTTGTACAAACGGTCGGTAGCTCAGTCTCACGTCGTTTTTTTTCTGCCATATGCAGTATATTTAGGTGATTTTTGTGTATATTTGTCTGATTTTTGTTTTTGATTTTAAATGTAGATCATGTACAGACTCGTTTAGCCATCATCATATTAGCTAGCTGGTTTTTAGTTGTACAGAGATGCAAACATTGGAGATGTAGGGTTTCTTTCTTCTTGAACCTAAGTTTTGCGGCCCAGGTATGTTTCATCGGCCGTTTTAGTTCTCTCACTTTCCGTACCGTTGAAATAATAATTATATCAGAAATGAAATAATAAATAATATAACATAATATATATAGGCTAAATCAGATTTAACCGTTAGAATTTGAAATTTGTGTCTATTTGTATTTTTAATGTAATAGATCA

mRNA sequence

AAGGCAACGCAGAAACTGACGACGTGTCGGCTCAGTTCTCTCAGTTCTTTAGGTAAAGGACGAGCAGAGTGTGAGCGGCAGATTCTTAATCAAAATCAACTAAGAAGCCATGGCCGGTATTCATCAGCCCGCTAATGCGTCGGTGAAGAACGGTCTTCCTCCCCAGGAGCTCCTTGATGATCTTTGCAGCCGATTTGTTTTAAATGTGCCAAGGGAAGATCTACAATCATTTGAGAGGATTCTGTTTCTAGTTGAATATGCACATTGGTTCTATGAGGACAACTCGGTAGAGAATAACCCTTCACTGAAGTCCTTCAACTTGAAGGAGTTCACTTCGTTGCTATTTAACAGTTGTGACGTTTTGAAACCATATGTGCCCCACATAGATGACATATTTAAGGACTTCACTTCCTATAAGCTTCGAGTTCCTGTCACTGGGGGAATTATTTTGGATGAAACCTTTGAGAGGTGCTTACTGGTGAAGGGATGGAAAGGGTCAAGCTGGAGTTTTCCTCGTGGAAAAAAGAGCAAGGATGAAGAAGATCATGCTTGTGCCATTAGAGAAGTTTTGGAGGAAACTGGTTTTGACGTGACACCGTTCCTCATCGAAGATGATTTTCTTGAAGTGATGTTTGGACAACAGAGAGTGCGGCTTTACATTATAGCTGGGGTGAAAACTGATATCCCCTTTGCACCTCTAACGAAGAAAGAGATCAGTGAAATTGCATGGCATCGACTTGACGACCTTCTACCCGCAAGTGACGATGTGATATCTCATGGAATCACCGGCCTCAAGCTTTACATGGTGGCTCCTTTCTTGAACTCGTTGAGATCATGGATCTTGAATCATCAGCCGCCTGTGAATCCGAACTTCGACATGCATGTAAAAGGAGTCACCATGTGGAAGGTGAAGAACAGCTCGATCGTTTTCGACAGCCAGCAAACAAAATTTGATTCTGATCCTGGACAAAGCCTCAGAAACTTCAAATTCGATACAGCCTCTATCTTGCAGGCTATGGAAAGTGCTTTTGCTGCTTGAGTTTCAACAAAAGCCTCGAACGCCTCTTGTACAAACGGTCGGTAGCTCAGTCTCACGTCGTTTTTTTTCTGCCATATGCAGTATATTTAGGTGATTTTTGTGTATATTTGTCTGATTTTTGTTTTTGATTTTAAATGTAGATCATGTACAGACTCGTTTAGCCATCATCATATTAGCTAGCTGGTTTTTAGTTGTACAGAGATGCAAACATTGGAGATGTAGGGTTTCTTTCTTCTTGAACCTAAGTTTTGCGGCCCAGGTATGTTTCATCGGCCGTTTTAGTTCTCTCACTTTCCGTACCGTTGAAATAATAATTATATCAGAAATGAAATAATAAATAATATAACATAATATATATAGGCTAAATCAGATTTAACCGTTAGAATTTGAAATTTGTGTCTATTTGTATTTTTAATGTAATAGATCA

Coding sequence (CDS)

ATGGCCGGTATTCATCAGCCCGCTAATGCGTCGGTGAAGAACGGTCTTCCTCCCCAGGAGCTCCTTGATGATCTTTGCAGCCGATTTGTTTTAAATGTGCCAAGGGAAGATCTACAATCATTTGAGAGGATTCTGTTTCTAGTTGAATATGCACATTGGTTCTATGAGGACAACTCGGTAGAGAATAACCCTTCACTGAAGTCCTTCAACTTGAAGGAGTTCACTTCGTTGCTATTTAACAGTTGTGACGTTTTGAAACCATATGTGCCCCACATAGATGACATATTTAAGGACTTCACTTCCTATAAGCTTCGAGTTCCTGTCACTGGGGGAATTATTTTGGATGAAACCTTTGAGAGGTGCTTACTGGTGAAGGGATGGAAAGGGTCAAGCTGGAGTTTTCCTCGTGGAAAAAAGAGCAAGGATGAAGAAGATCATGCTTGTGCCATTAGAGAAGTTTTGGAGGAAACTGGTTTTGACGTGACACCGTTCCTCATCGAAGATGATTTTCTTGAAGTGATGTTTGGACAACAGAGAGTGCGGCTTTACATTATAGCTGGGGTGAAAACTGATATCCCCTTTGCACCTCTAACGAAGAAAGAGATCAGTGAAATTGCATGGCATCGACTTGACGACCTTCTACCCGCAAGTGACGATGTGATATCTCATGGAATCACCGGCCTCAAGCTTTACATGGTGGCTCCTTTCTTGAACTCGTTGAGATCATGGATCTTGAATCATCAGCCGCCTGTGAATCCGAACTTCGACATGCATGTAAAAGGAGTCACCATGTGGAAGGTGAAGAACAGCTCGATCGTTTTCGACAGCCAGCAAACAAAATTTGATTCTGATCCTGGACAAAGCCTCAGAAACTTCAAATTCGATACAGCCTCTATCTTGCAGGCTATGGAAAGTGCTTTTGCTGCTTGA

Protein sequence

MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRVRLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSLRSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASILQAMESAFAA
Homology
BLAST of Carg01954 vs. NCBI nr
Match: XP_022956451.1 (mRNA-decapping enzyme subunit 2-like [Cucurbita moschata] >KAG7031966.1 mRNA-decapping enzyme subunit 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 638.3 bits (1645), Expect = 3.5e-179
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL
Sbjct: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL 300
           RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL
Sbjct: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL 300

Query: 301 QAMESAFAA 310
           QAMESAFAA
Sbjct: 301 QAMESAFAA 309

BLAST of Carg01954 vs. NCBI nr
Match: XP_023523225.1 (mRNA-decapping enzyme subunit 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 637.9 bits (1644), Expect = 4.6e-179
Identity = 308/309 (99.68%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFL+EDDFLEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLVEDDFLEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL
Sbjct: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL 300
           RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL
Sbjct: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL 300

Query: 301 QAMESAFAA 310
           QAMESAFAA
Sbjct: 301 QAMESAFAA 309

BLAST of Carg01954 vs. NCBI nr
Match: XP_022977496.1 (mRNA-decapping enzyme subunit 2-like [Cucurbita maxima])

HSP 1 Score: 632.9 bits (1631), Expect = 1.5e-177
Identity = 306/309 (99.03%), Postives = 307/309 (99.35%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL
Sbjct: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL 300
           RSWIL HQPPVNPNFDMHVKGVTMWKVKNSSIVFD QQTKFDSDPG+SLRNFKFDTASIL
Sbjct: 241 RSWILKHQPPVNPNFDMHVKGVTMWKVKNSSIVFDGQQTKFDSDPGKSLRNFKFDTASIL 300

Query: 301 QAMESAFAA 310
           QAMESAFAA
Sbjct: 301 QAMESAFAA 309

BLAST of Carg01954 vs. NCBI nr
Match: XP_004142410.1 (mRNA-decapping enzyme subunit 2 [Cucumis sativus] >XP_031742317.1 mRNA-decapping enzyme subunit 2 [Cucumis sativus] >KGN52271.1 hypothetical protein Csa_007962 [Cucumis sativus])

HSP 1 Score: 586.6 bits (1511), Expect = 1.2e-163
Identity = 285/314 (90.76%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MAG+H P+NA VKNGLPPQELLDDLCSRFVLNVP+EDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLI++DF+EVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVK D  FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVK-----NSSIVFDSQQTKFDSDPGQSLRNFKFD 300
           RSWIL HQPPV PNFDM VKG TMWKVK     NSSI+FD+Q  K DSDPG+SLRNFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300

Query: 301 TASILQAMESAFAA 310
           TASIL AMESAFAA
Sbjct: 301 TASILHAMESAFAA 314

BLAST of Carg01954 vs. NCBI nr
Match: XP_008446886.1 (PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0034722.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa] >TYK09275.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 583.2 bits (1502), Expect = 1.3e-162
Identity = 283/314 (90.13%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MA +H P+NA VKNGLPPQELLDDLCSRFVLNVP+EDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MASLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLF+SCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFSSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLI++DF+EVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVK D  FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVK-----NSSIVFDSQQTKFDSDPGQSLRNFKFD 300
           RSWIL HQPPV PNFDM VKG TMWKVK     NSSI+FD+Q  K DSDPG+SL+NFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGYTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLKNFKFD 300

Query: 301 TASILQAMESAFAA 310
           TASILQAMESAFAA
Sbjct: 301 TASILQAMESAFAA 314

BLAST of Carg01954 vs. ExPASy Swiss-Prot
Match: Q8GW31 (mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 5.7e-116
Identity = 198/272 (72.79%), Postives = 232/272 (85.29%), Query Frame = 0

Query: 1   MAGIHQPANAS--VKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDN 60
           M+G+H+ +++S  + N LP +ELLDDLCSRFVLNVP ED QSFERILFLVEYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF 120
           +VEN+P LKS +LKEFTSLLFNSCDVL+PYV HIDDIFKDFTSYK RVPVTG IILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+  L  ++++E +F QQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 181 RVRLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLN 240
           RVRLYI+AGV  D  FAPLTKKEISEI WHRLD L PAS++VI+HG++GLKLYMVAPFL+
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 241 SLRSWILNHQPPVNPNFDMHVKGVTMWKVKNS 271
           SL+SWIL H  PV    +  +K + +W  + S
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKALCVWNARTS 272

BLAST of Carg01954 vs. ExPASy Swiss-Prot
Match: O13828 (mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dcp2 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 4.5e-52
Identity = 105/226 (46.46%), Postives = 139/226 (61.50%), Query Frame = 0

Query: 20  ELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSLLF 79
           ++LDDL +RF+LN+P E+  S ER+ F +E AHWFYED     N  L S  L+ F++ LF
Sbjct: 10  QVLDDLSARFILNLPAEEQSSVERLCFQIEQAHWFYEDFIRAQNDQLPSLGLRVFSAKLF 69

Query: 80  NSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKGSS-WSFPRGK 139
             C +L  +    ++ F DF  YK R+PV G I+LD + ++C+LVKGWK SS W FP+GK
Sbjct: 70  AHCPLLWKWSKVHEEAFDDFLRYKTRIPVRGAIMLDMSMQQCVLVKGWKASSGWGFPKGK 129

Query: 140 KSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRVRLYIIAGVKTDIPFAPLT 199
             KDE D  CAIREV EETGFD +  +  ++F+++    Q VRLYII G+  D  F   T
Sbjct: 130 IDKDESDVDCAIREVYEETGFDCSSRINPNEFIDMTIRGQNVRLYIIPGISLDTRFESRT 189

Query: 200 KKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSLRSWI 245
           +KEIS+I WH L DL     +         K YMV PFL  L+ WI
Sbjct: 190 RKEISKIEWHNLMDLPTFKKN--KPQTMKNKFYMVIPFLAPLKKWI 233

BLAST of Carg01954 vs. ExPASy Swiss-Prot
Match: Q9CYC6 (m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2)

HSP 1 Score: 203.4 bits (516), Expect = 3.8e-51
Identity = 96/231 (41.56%), Postives = 137/231 (59.31%), Query Frame = 0

Query: 18  PQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSL 77
           P  +LDDLCSRF+L++P E+  +  R+ F +E AHWFY D  ++N P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 78  LFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKG-SSWSFPR 137
           +F+ C  L P    ++ I  ++  YK+ VP  G IILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKILDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 138 GKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRVRLYIIAGVKTDIPFAP 197
           GK +K+E  H CA REV EETGFD+  ++ +DD++E+    Q  RLYII GV  D  F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGVPKDTKFNP 188

Query: 198 LTKKEISEIAWHRLDDLLPASDDVISHGITGL---KLYMVAPFLNSLRSWI 245
            T++EI  I W  ++ L    +D+      GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of Carg01954 vs. ExPASy Swiss-Prot
Match: Q8IU60 (m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3)

HSP 1 Score: 202.6 bits (514), Expect = 6.5e-51
Identity = 94/231 (40.69%), Postives = 137/231 (59.31%), Query Frame = 0

Query: 18  PQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSL 77
           P  +LDDLCSRF+L++P E+  +  R+ F +E AHWFY D  ++N P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 78  LFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKG-SSWSFPR 137
           +F+ C  L P    ++ +  ++  YK+ VP  G IILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 138 GKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRVRLYIIAGVKTDIPFAP 197
           GK +K+E  H CA REV EETGFD+  ++ +DD++E+    Q  RLYII G+  D  F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 198 LTKKEISEIAWHRLDDLLPASDDVISHGITGL---KLYMVAPFLNSLRSWI 245
            T++EI  I W  ++ L    +D+      GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of Carg01954 vs. ExPASy Swiss-Prot
Match: Q5REQ8 (m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.5e-51
Identity = 94/231 (40.69%), Postives = 137/231 (59.31%), Query Frame = 0

Query: 18  PQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSL 77
           P  +LDDLCSRF+L++P E+  +  R+ F +E AHWFY D  ++N P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLSQCGIRDFAKA 68

Query: 78  LFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKG-SSWSFPR 137
           +F+ C  L P    ++ +  ++  YK+ VP  G IILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPEGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 138 GKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRVRLYIIAGVKTDIPFAP 197
           GK +K+E  H CA REV EETGFD+  ++ +DD++E+    Q  RLYII G+  D  F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 198 LTKKEISEIAWHRLDDLLPASDDVISHGITGL---KLYMVAPFLNSLRSWI 245
            T++EI  I W  ++ L    +D+      GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of Carg01954 vs. ExPASy TrEMBL
Match: A0A6J1GWL4 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458180 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 1.7e-179
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL
Sbjct: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL 300
           RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL
Sbjct: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL 300

Query: 301 QAMESAFAA 310
           QAMESAFAA
Sbjct: 301 QAMESAFAA 309

BLAST of Carg01954 vs. ExPASy TrEMBL
Match: A0A6J1IK27 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477819 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 7.1e-178
Identity = 306/309 (99.03%), Postives = 307/309 (99.35%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL
Sbjct: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVKNSSIVFDSQQTKFDSDPGQSLRNFKFDTASIL 300
           RSWIL HQPPVNPNFDMHVKGVTMWKVKNSSIVFD QQTKFDSDPG+SLRNFKFDTASIL
Sbjct: 241 RSWILKHQPPVNPNFDMHVKGVTMWKVKNSSIVFDGQQTKFDSDPGKSLRNFKFDTASIL 300

Query: 301 QAMESAFAA 310
           QAMESAFAA
Sbjct: 301 QAMESAFAA 309

BLAST of Carg01954 vs. ExPASy TrEMBL
Match: A0A0A0KWT4 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G622860 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 5.9e-164
Identity = 285/314 (90.76%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MAG+H P+NA VKNGLPPQELLDDLCSRFVLNVP+EDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLI++DF+EVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVK D  FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVK-----NSSIVFDSQQTKFDSDPGQSLRNFKFD 300
           RSWIL HQPPV PNFDM VKG TMWKVK     NSSI+FD+Q  K DSDPG+SLRNFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300

Query: 301 TASILQAMESAFAA 310
           TASIL AMESAFAA
Sbjct: 301 TASILHAMESAFAA 314

BLAST of Carg01954 vs. ExPASy TrEMBL
Match: A0A5D3CCQ6 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002700 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.5e-163
Identity = 283/314 (90.13%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MA +H P+NA VKNGLPPQELLDDLCSRFVLNVP+EDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MASLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLF+SCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFSSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLI++DF+EVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVK D  FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVK-----NSSIVFDSQQTKFDSDPGQSLRNFKFD 300
           RSWIL HQPPV PNFDM VKG TMWKVK     NSSI+FD+Q  K DSDPG+SL+NFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGYTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLKNFKFD 300

Query: 301 TASILQAMESAFAA 310
           TASILQAMESAFAA
Sbjct: 301 TASILQAMESAFAA 314

BLAST of Carg01954 vs. ExPASy TrEMBL
Match: A0A1S3BG59 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103489466 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.5e-163
Identity = 283/314 (90.13%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60
           MA +H P+NA VKNGLPPQELLDDLCSRFVLNVP+EDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MASLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLF+SCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFSSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLI++DF+EVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240
           RLYIIAGVK D  FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILNHQPPVNPNFDMHVKGVTMWKVK-----NSSIVFDSQQTKFDSDPGQSLRNFKFD 300
           RSWIL HQPPV PNFDM VKG TMWKVK     NSSI+FD+Q  K DSDPG+SL+NFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGYTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLKNFKFD 300

Query: 301 TASILQAMESAFAA 310
           TASILQAMESAFAA
Sbjct: 301 TASILQAMESAFAA 314

BLAST of Carg01954 vs. TAIR 10
Match: AT5G13570.1 (decapping 2 )

HSP 1 Score: 418.7 bits (1075), Expect = 4.1e-117
Identity = 198/272 (72.79%), Postives = 232/272 (85.29%), Query Frame = 0

Query: 1   MAGIHQPANAS--VKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDN 60
           M+G+H+ +++S  + N LP +ELLDDLCSRFVLNVP ED QSFERILFLVEYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF 120
           +VEN+P LKS +LKEFTSLLFNSCDVL+PYV HIDDIFKDFTSYK RVPVTG IILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+  L  ++++E +F QQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 181 RVRLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLN 240
           RVRLYI+AGV  D  FAPLTKKEISEI WHRLD L PAS++VI+HG++GLKLYMVAPFL+
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 241 SLRSWILNHQPPVNPNFDMHVKGVTMWKVKNS 271
           SL+SWIL H  PV    +  +K + +W  + S
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKALCVWNARTS 272

BLAST of Carg01954 vs. TAIR 10
Match: AT5G13570.2 (decapping 2 )

HSP 1 Score: 409.5 bits (1051), Expect = 2.5e-114
Identity = 198/285 (69.47%), Postives = 232/285 (81.40%), Query Frame = 0

Query: 1   MAGIHQPANAS--VKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDN 60
           M+G+H+ +++S  + N LP +ELLDDLCSRFVLNVP ED QSFERILFLVEYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF 120
           +VEN+P LKS +LKEFTSLLFNSCDVL+PYV HIDDIFKDFTSYK RVPVTG IILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE-------------VLEETGFDVTPFL 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE             VLEETGFDV+  L
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRELSSAILLVNVAFQVLEETGFDVSKLL 180

Query: 181 IEDDFLEVMFGQQRVRLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGI 240
             ++++E +F QQRVRLYI+AGV  D  FAPLTKKEISEI WHRLD L PAS++VI+HG+
Sbjct: 181 KREEYIEFVFRQQRVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGV 240

Query: 241 TGLKLYMVAPFLNSLRSWILNHQPPVNPNFDMHVKGVTMWKVKNS 271
           +GLKLYMVAPFL+SL+SWIL H  PV    +  +K + +W  + S
Sbjct: 241 SGLKLYMVAPFLSSLKSWILKHPSPVARRPNKPLKALCVWNARTS 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022956451.13.5e-179100.00mRNA-decapping enzyme subunit 2-like [Cucurbita moschata] >KAG7031966.1 mRNA-dec... [more]
XP_023523225.14.6e-17999.68mRNA-decapping enzyme subunit 2-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022977496.11.5e-17799.03mRNA-decapping enzyme subunit 2-like [Cucurbita maxima][more]
XP_004142410.11.2e-16390.76mRNA-decapping enzyme subunit 2 [Cucumis sativus] >XP_031742317.1 mRNA-decapping... [more]
XP_008446886.11.3e-16290.13PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0034722.1 mRN... [more]
Match NameE-valueIdentityDescription
Q8GW315.7e-11672.79mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=... [more]
O138284.5e-5246.46mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q9CYC63.8e-5141.56m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2[more]
Q8IU606.5e-5140.69m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3[more]
Q5REQ86.5e-5140.69m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GWL41.7e-179100.00mRNA-decapping enzyme subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114581... [more]
A0A6J1IK277.1e-17899.03mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477819... [more]
A0A0A0KWT45.9e-16490.76Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G62... [more]
A0A5D3CCQ66.5e-16390.13mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BG596.5e-16390.13mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103489466 PE=... [more]
Match NameE-valueIdentityDescription
AT5G13570.14.1e-11772.79decapping 2 [more]
AT5G13570.22.5e-11469.47decapping 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007722mRNA decapping protein 2, Box A domainSMARTSM01125DCP2_2coord: 19..103
e-value: 5.8E-43
score: 158.7
IPR007722mRNA decapping protein 2, Box A domainPFAMPF05026DCP2coord: 20..102
e-value: 3.3E-28
score: 97.8
IPR036189mRNA decapping protein 2, Box A domain superfamilyGENE3D1.10.10.1050Dcp2, box A domaincoord: 21..104
e-value: 8.0E-85
score: 285.6
IPR036189mRNA decapping protein 2, Box A domain superfamilySUPERFAMILY140586Dcp2 domain-likecoord: 14..103
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 105..245
e-value: 8.0E-85
score: 285.6
NoneNo IPR availablePANTHERPTHR23114M7GPPPN-MRNA HYDROLASEcoord: 13..261
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 107..217
e-value: 6.1E-13
score: 48.9
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 104..233
score: 13.558689
IPR020084NUDIX hydrolase, conserved sitePROSITEPS00893NUDIX_BOXcoord: 137..158
IPR044099mRNA decapping enzyme 2 , NUDIX hydrolase domainCDDcd03672Dcp2pcoord: 106..248
e-value: 4.58886E-74
score: 222.498
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 106..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01954-RACarg01954-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA
biological_process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050072 m7G(5')pppN diphosphatase activity
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0016787 hydrolase activity