Carg01943 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01943
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionE3 ubiquitin ligase complex SCF subunit
LocationCarg_Chr04: 8464714 .. 8465202 (+)
RNA-Seq ExpressionCarg01943
SyntenyCarg01943
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAACTTTTCAACTAAAATCATCGGACAACAAAATTTTCAAGGTAAGCGAGGAGATCGCGATACAATCCGCAGTGATTAAGAGCTTTCTTGAAGATTTCGGATCTGATAGCGACGAAATTGTGATTCCGCTGCCAAATGTTAGCGGAAGAATATTAGAGATAGTGATTGAGTGGATCGTGAAGCACGCGAATGATGAACTGACGAAAGAAGAAGTCCAAGATTGGGAGAATAAGTTTATGAATGATCTAAATATGGAAGTGACGACGGAGCTTGTAATGGCGGCCAATTACCTCGAAGTCATCGGATTGCTCCATCGAACTTGCCAGAGCATCGCCAATCAAATTTCCGGCAAGAGTCCGGAGGAGATTCGGAGGATACTTAACATTACCAATGATTTTACGCCGGAAGAAGAGGCGGAGATCCGAGAACAGAATGGTTGGGATCCCAATCCATCAACAACGGACGACGGGATTTTTCCGATTTAA

mRNA sequence

ATGAGAACTTTTCAACTAAAATCATCGGACAACAAAATTTTCAAGGTAAGCGAGGAGATCGCGATACAATCCGCAGTGATTAAGAGCTTTCTTGAAGATTTCGGATCTGATAGCGACGAAATTGTGATTCCGCTGCCAAATGTTAGCGGAAGAATATTAGAGATAGTGATTGAGTGGATCGTGAAGCACGCGAATGATGAACTGACGAAAGAAGAAGTCCAAGATTGGGAGAATAAGTTTATGAATGATCTAAATATGGAAGTGACGACGGAGCTTGTAATGGCGGCCAATTACCTCGAAGTCATCGGATTGCTCCATCGAACTTGCCAGAGCATCGCCAATCAAATTTCCGGCAAGAGTCCGGAGGAGATTCGGAGGATACTTAACATTACCAATGATTTTACGCCGGAAGAAGAGGCGGAGATCCGAGAACAGAATGGTTGGGATCCCAATCCATCAACAACGGACGACGGGATTTTTCCGATTTAA

Coding sequence (CDS)

ATGAGAACTTTTCAACTAAAATCATCGGACAACAAAATTTTCAAGGTAAGCGAGGAGATCGCGATACAATCCGCAGTGATTAAGAGCTTTCTTGAAGATTTCGGATCTGATAGCGACGAAATTGTGATTCCGCTGCCAAATGTTAGCGGAAGAATATTAGAGATAGTGATTGAGTGGATCGTGAAGCACGCGAATGATGAACTGACGAAAGAAGAAGTCCAAGATTGGGAGAATAAGTTTATGAATGATCTAAATATGGAAGTGACGACGGAGCTTGTAATGGCGGCCAATTACCTCGAAGTCATCGGATTGCTCCATCGAACTTGCCAGAGCATCGCCAATCAAATTTCCGGCAAGAGTCCGGAGGAGATTCGGAGGATACTTAACATTACCAATGATTTTACGCCGGAAGAAGAGGCGGAGATCCGAGAACAGAATGGTTGGGATCCCAATCCATCAACAACGGACGACGGGATTTTTCCGATTTAA

Protein sequence

MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWIVKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKSPEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI
Homology
BLAST of Carg01943 vs. NCBI nr
Match: KAG7031977.1 (S-phase kinase-associated protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 320.5 bits (820), Expect = 8.5e-84
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI
Sbjct: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
           VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS
Sbjct: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI 163
           PEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI
Sbjct: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI 162

BLAST of Carg01943 vs. NCBI nr
Match: KAG6601179.1 (S-phase kinase-associated protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 318.5 bits (815), Expect = 3.2e-83
Identity = 161/162 (99.38%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI
Sbjct: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
           VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIA+QISGKS
Sbjct: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIADQISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI 163
           PEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI
Sbjct: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI 162

BLAST of Carg01943 vs. NCBI nr
Match: XP_023549855.1 (S-phase kinase-associated protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 308.5 bits (789), Expect = 3.3e-80
Identity = 157/162 (96.91%), Postives = 160/162 (98.77%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI
Sbjct: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
           VKHA+DELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIA+QISGKS
Sbjct: 61  VKHADDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIADQISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI 163
           PEEIRRILNITNDFTPEEEAEIREQNGWD NPSTTDDG+F I
Sbjct: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDLNPSTTDDGLFRI 162

BLAST of Carg01943 vs. NCBI nr
Match: XP_022956819.1 (S-phase kinase-associated protein 1-like [Cucurbita moschata])

HSP 1 Score: 308.5 bits (789), Expect = 3.3e-80
Identity = 157/162 (96.91%), Postives = 160/162 (98.77%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI
Sbjct: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
           VKHA+DELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIA+QISGKS
Sbjct: 61  VKHADDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIADQISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI 163
           PEEIRRILNITNDFTPEEEAEIREQNGWD NPSTTDD +FPI
Sbjct: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDLNPSTTDDEMFPI 162

BLAST of Carg01943 vs. NCBI nr
Match: XP_022989752.1 (S-phase kinase-associated protein 1-like [Cucurbita maxima])

HSP 1 Score: 275.4 bits (703), Expect = 3.1e-70
Identity = 139/154 (90.26%), Postives = 148/154 (96.10%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRTFQLKSSDNKIFKVSEEIAIQSAVIK+FLEDFGSDSDEIVIPLPNV GRILE++IEW+
Sbjct: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKNFLEDFGSDSDEIVIPLPNVRGRILEMMIEWM 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
           VKH ++ELTKEEV+DWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIA+ ISGKS
Sbjct: 61  VKHVDEELTKEEVEDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIADHISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPST 155
           PEEIRRILNIT+DFTPEEEAEIR QNGW  NPST
Sbjct: 121 PEEIRRILNITSDFTPEEEAEIRRQNGWVLNPST 154

BLAST of Carg01943 vs. ExPASy Swiss-Prot
Match: Q9WTX5 (S-phase kinase-associated protein 1 OS=Mus musculus OX=10090 GN=Skp1 PE=1 SV=3)

HSP 1 Score: 135.2 bits (339), Expect = 6.6e-31
Identity = 72/160 (45.00%), Postives = 104/160 (65.00%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSD--SDEIVIPLPNVSGRILEIVIE 60
           M T +L+SSD +IF+V  EIA QS  IK+ LED G D   D+  +PLPNV+  IL+ VI+
Sbjct: 1   MPTIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ 60

Query: 61  WIVKHAND----------ELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRT 120
           W   H +D          E   +++  W+ +F+  ++     EL++AANYL++ GLL  T
Sbjct: 61  WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLK-VDQGTLFELILAANYLDIKGLLDVT 120

Query: 121 CQSIANQISGKSPEEIRRILNITNDFTPEEEAEIREQNGW 149
           C+++AN I GK+PEEIR+  NI NDFT EEEA++R++N W
Sbjct: 121 CKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQW 159

BLAST of Carg01943 vs. ExPASy Swiss-Prot
Match: Q6PEC4 (S-phase kinase-associated protein 1 OS=Rattus norvegicus OX=10116 GN=Skp1 PE=1 SV=3)

HSP 1 Score: 135.2 bits (339), Expect = 6.6e-31
Identity = 72/160 (45.00%), Postives = 104/160 (65.00%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSD--SDEIVIPLPNVSGRILEIVIE 60
           M T +L+SSD +IF+V  EIA QS  IK+ LED G D   D+  +PLPNV+  IL+ VI+
Sbjct: 1   MPTIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ 60

Query: 61  WIVKHAND----------ELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRT 120
           W   H +D          E   +++  W+ +F+  ++     EL++AANYL++ GLL  T
Sbjct: 61  WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLK-VDQGTLFELILAANYLDIKGLLDVT 120

Query: 121 CQSIANQISGKSPEEIRRILNITNDFTPEEEAEIREQNGW 149
           C+++AN I GK+PEEIR+  NI NDFT EEEA++R++N W
Sbjct: 121 CKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQW 159

BLAST of Carg01943 vs. ExPASy Swiss-Prot
Match: Q3ZCF3 (S-phase kinase-associated protein 1 OS=Bos taurus OX=9913 GN=SKP1 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.9e-30
Identity = 71/160 (44.38%), Postives = 104/160 (65.00%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSD--SDEIVIPLPNVSGRILEIVIE 60
           M + +L+SSD +IF+V  EIA QS  IK+ LED G D   D+  +PLPNV+  IL+ VI+
Sbjct: 1   MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ 60

Query: 61  WIVKHAND----------ELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRT 120
           W   H +D          E   +++  W+ +F+  ++     EL++AANYL++ GLL  T
Sbjct: 61  WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLK-VDQGTLFELILAANYLDIKGLLDVT 120

Query: 121 CQSIANQISGKSPEEIRRILNITNDFTPEEEAEIREQNGW 149
           C+++AN I GK+PEEIR+  NI NDFT EEEA++R++N W
Sbjct: 121 CKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQW 159

BLAST of Carg01943 vs. ExPASy Swiss-Prot
Match: P63209 (S-phase kinase-associated protein 1 OS=Cavia porcellus OX=10141 GN=SKP1 PE=2 SV=2)

HSP 1 Score: 133.7 bits (335), Expect = 1.9e-30
Identity = 71/160 (44.38%), Postives = 104/160 (65.00%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSD--SDEIVIPLPNVSGRILEIVIE 60
           M + +L+SSD +IF+V  EIA QS  IK+ LED G D   D+  +PLPNV+  IL+ VI+
Sbjct: 1   MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ 60

Query: 61  WIVKHAND----------ELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRT 120
           W   H +D          E   +++  W+ +F+  ++     EL++AANYL++ GLL  T
Sbjct: 61  WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLK-VDQGTLFELILAANYLDIKGLLDVT 120

Query: 121 CQSIANQISGKSPEEIRRILNITNDFTPEEEAEIREQNGW 149
           C+++AN I GK+PEEIR+  NI NDFT EEEA++R++N W
Sbjct: 121 CKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQW 159

BLAST of Carg01943 vs. ExPASy Swiss-Prot
Match: Q5ZKF5 (S-phase kinase-associated protein 1 OS=Gallus gallus OX=9031 GN=SKP1 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.9e-30
Identity = 71/160 (44.38%), Postives = 104/160 (65.00%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSD--SDEIVIPLPNVSGRILEIVIE 60
           M + +L+SSD +IF+V  EIA QS  IK+ LED G D   D+  +PLPNV+  IL+ VI+
Sbjct: 1   MPSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQ 60

Query: 61  WIVKHAND----------ELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRT 120
           W   H +D          E   +++  W+ +F+  ++     EL++AANYL++ GLL  T
Sbjct: 61  WCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLK-VDQGTLFELILAANYLDIKGLLDVT 120

Query: 121 CQSIANQISGKSPEEIRRILNITNDFTPEEEAEIREQNGW 149
           C+++AN I GK+PEEIR+  NI NDFT EEEA++R++N W
Sbjct: 121 CKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQW 159

BLAST of Carg01943 vs. ExPASy TrEMBL
Match: A0A6J1H051 (SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458404 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.6e-80
Identity = 157/162 (96.91%), Postives = 160/162 (98.77%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI
Sbjct: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
           VKHA+DELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIA+QISGKS
Sbjct: 61  VKHADDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIADQISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPSTTDDGIFPI 163
           PEEIRRILNITNDFTPEEEAEIREQNGWD NPSTTDD +FPI
Sbjct: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDLNPSTTDDEMFPI 162

BLAST of Carg01943 vs. ExPASy TrEMBL
Match: A0A6J1JGP3 (SKP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111486777 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.5e-70
Identity = 139/154 (90.26%), Postives = 148/154 (96.10%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRTFQLKSSDNKIFKVSEEIAIQSAVIK+FLEDFGSDSDEIVIPLPNV GRILE++IEW+
Sbjct: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKNFLEDFGSDSDEIVIPLPNVRGRILEMMIEWM 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
           VKH ++ELTKEEV+DWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIA+ ISGKS
Sbjct: 61  VKHVDEELTKEEVEDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIADHISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPST 155
           PEEIRRILNIT+DFTPEEEAEIR QNGW  NPST
Sbjct: 121 PEEIRRILNITSDFTPEEEAEIRRQNGWVLNPST 154

BLAST of Carg01943 vs. ExPASy TrEMBL
Match: A0A5A7SU15 (SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007240 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 6.9e-47
Identity = 94/153 (61.44%), Postives = 122/153 (79.74%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRT  L+SS+N+IFKVSEE+A QS V+++FLE+  S+ DEI IPLPN+SG++L +VIEWI
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
             H  ++L +E++  W+ KFM DL++++  EL+MAANYLEV  L H TCQ +A++ISGKS
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPS 154
           PEEIR+I NITNDFTPEEEAEIR QN W   PS
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEPS 153

BLAST of Carg01943 vs. ExPASy TrEMBL
Match: A0A5D3CCN6 (SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002490 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 6.9e-47
Identity = 94/153 (61.44%), Postives = 122/153 (79.74%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRT  L+SS+N+IFKVSEE+A QS V+++FLE+  S+ DEI IPLPN+SG++L +VIEWI
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
             H  ++L +E++  W+ KFM DL++++  EL+MAANYLEV  L H TCQ +A++ISGKS
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPS 154
           PEEIR+I NITNDFTPEEEAEIR QN W   PS
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEPS 153

BLAST of Carg01943 vs. ExPASy TrEMBL
Match: A0A1S3BGP6 (SKP1-like protein OS=Cucumis melo OX=3656 GN=LOC103489446 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 6.9e-47
Identity = 94/153 (61.44%), Postives = 122/153 (79.74%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           MRT  L+SS+N+IFKVSEE+A QS V+++FLE+  S+ DEI IPLPN+SG++L +VIEWI
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 61  VKHANDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKS 120
             H  ++L +E++  W+ KFM DL++++  EL+MAANYLEV  L H TCQ +A++ISGKS
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDPNPS 154
           PEEIR+I NITNDFTPEEEAEIR QN W   PS
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEPS 153

BLAST of Carg01943 vs. TAIR 10
Match: AT4G34210.1 (SKP1-like 11 )

HSP 1 Score: 123.2 bits (308), Expect = 1.9e-28
Identity = 70/146 (47.95%), Postives = 93/146 (63.70%), Query Frame = 0

Query: 6   LKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWIVKHAN 65
           L SSD + F+V E +AIQS  I   +ED   D     IPL NV  +IL  VIE+  KH  
Sbjct: 8   LMSSDGQSFEVEEAVAIQSQTIAHMVED---DCVADGIPLANVESKILVKVIEYCKKHHV 67

Query: 66  DE---LTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKSPE 125
           DE   +++E++ +W+ KFM DL      EL++AANYL +  LL  TCQ++A+ I GK+PE
Sbjct: 68  DEANPISEEDLNNWDEKFM-DLEQSTIFELILAANYLNIKSLLDLTCQTVADMIKGKTPE 127

Query: 126 EIRRILNITNDFTPEEEAEIREQNGW 149
           EIR   NI NDFTPEEE  +R++N W
Sbjct: 128 EIRSTFNIENDFTPEEEEAVRKENQW 149

BLAST of Carg01943 vs. TAIR 10
Match: AT2G03170.1 (SKP1-like 14 )

HSP 1 Score: 120.9 bits (302), Expect = 9.2e-28
Identity = 62/143 (43.36%), Postives = 93/143 (65.03%), Query Frame = 0

Query: 6   LKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWIVKHAN 65
           L SSD + F+V E +A +  +++  +ED   D     +PL NV+G+IL IV+E+  KH  
Sbjct: 8   LSSSDGESFEVEEAVARKLKIVEHMIED---DCVVTEVPLQNVTGKILSIVVEYCKKHVV 67

Query: 66  DELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKSPEEIR 125
           DE   +E + W+ +FM   +     +L++AANYL + GLL  + Q++A++I  K+PEEIR
Sbjct: 68  DE-ESDEFKTWDEEFMKKFDQPTVFQLLLAANYLNIKGLLDLSAQTVADRIKDKTPEEIR 127

Query: 126 RILNITNDFTPEEEAEIREQNGW 149
            I NI NDFTPEEEA +R++N W
Sbjct: 128 EIFNIENDFTPEEEAAVRKENAW 146

BLAST of Carg01943 vs. TAIR 10
Match: AT1G20140.1 (SKP1-like 4 )

HSP 1 Score: 119.0 bits (297), Expect = 3.5e-27
Identity = 63/155 (40.65%), Postives = 99/155 (63.87%), Query Frame = 0

Query: 6   LKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWIVKHAN 65
           LKSSD + F++ E +A++S  IK  +ED  +D+    IPLPNV+G IL  VIE+  KH  
Sbjct: 10  LKSSDGESFEIEEAVAVKSQTIKHMIEDDCADNG---IPLPNVTGAILAKVIEYCKKHVE 69

Query: 66  ------------DELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIA 125
                            +E+++W+++F+  ++     +L++AANYL + GLL  TC+++A
Sbjct: 70  AAAEAGGDKDFYGSAENDELKNWDSEFVK-VDQPTLFDLILAANYLNIGGLLDLTCKAVA 129

Query: 126 NQISGKSPEEIRRILNITNDFTPEEEAEIREQNGW 149
           +Q+ GK+PE++R   NI ND+TPEEEAE+R +N W
Sbjct: 130 DQMRGKTPEQMRAHFNIKNDYTPEEEAEVRNENKW 160

BLAST of Carg01943 vs. TAIR 10
Match: AT4G34470.1 (SKP1-like 12 )

HSP 1 Score: 118.6 bits (296), Expect = 4.6e-27
Identity = 68/146 (46.58%), Postives = 91/146 (62.33%), Query Frame = 0

Query: 6   LKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWIVKHAN 65
           L SSD + F+V E +AIQS  I   +ED   D     IPL NV  +IL  VIE+  K+  
Sbjct: 8   LMSSDGQSFEVEEAVAIQSQTIAHMVED---DCVADGIPLANVESKILVKVIEYCKKYHV 67

Query: 66  DE---LTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIANQISGKSPE 125
           DE   +++E++  W+ KFM DL      EL++AANYL +  L   TCQ++A+ I GK+PE
Sbjct: 68  DEANPISEEDLNKWDEKFM-DLEQSTIFELILAANYLNIKSLFDLTCQTVADMIKGKTPE 127

Query: 126 EIRRILNITNDFTPEEEAEIREQNGW 149
           EIR   NI NDFTPEEE  +R++N W
Sbjct: 128 EIRSTFNIENDFTPEEEEAVRKENQW 149

BLAST of Carg01943 vs. TAIR 10
Match: AT5G42190.1 (E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein )

HSP 1 Score: 118.6 bits (296), Expect = 4.6e-27
Identity = 67/169 (39.64%), Postives = 101/169 (59.76%), Query Frame = 0

Query: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60
           +R   LKSSD + F++ E +A++S  IK  +ED  +D+    IPLPNV+ +IL  VIE+ 
Sbjct: 4   VRKITLKSSDGENFEIDEAVALESQTIKHMIEDDCTDNG---IPLPNVTSKILSKVIEYC 63

Query: 61  VKHA----NDELT-----------------KEEVQDWENKFMNDLNMEVTTELVMAANYL 120
            +H       E T                  E+++ W+++F+  ++     +L++AANYL
Sbjct: 64  KRHVEAAEKSETTADAAAATTTTTVASGSSDEDLKTWDSEFIK-VDQGTLFDLILAANYL 123

Query: 121 EVIGLLHRTCQSIANQISGKSPEEIRRILNITNDFTPEEEAEIREQNGW 149
            + GLL  TCQ++A+ I GK+PEEIR+  NI NDFTPEEE E+R +N W
Sbjct: 124 NIKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQW 168

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031977.18.5e-84100.00S-phase kinase-associated protein 1, partial [Cucurbita argyrosperma subsp. argy... [more]
KAG6601179.13.2e-8399.38S-phase kinase-associated protein 1, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_023549855.13.3e-8096.91S-phase kinase-associated protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_022956819.13.3e-8096.91S-phase kinase-associated protein 1-like [Cucurbita moschata][more]
XP_022989752.13.1e-7090.26S-phase kinase-associated protein 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9WTX56.6e-3145.00S-phase kinase-associated protein 1 OS=Mus musculus OX=10090 GN=Skp1 PE=1 SV=3[more]
Q6PEC46.6e-3145.00S-phase kinase-associated protein 1 OS=Rattus norvegicus OX=10116 GN=Skp1 PE=1 S... [more]
Q3ZCF31.9e-3044.38S-phase kinase-associated protein 1 OS=Bos taurus OX=9913 GN=SKP1 PE=2 SV=1[more]
P632091.9e-3044.38S-phase kinase-associated protein 1 OS=Cavia porcellus OX=10141 GN=SKP1 PE=2 SV=... [more]
Q5ZKF51.9e-3044.38S-phase kinase-associated protein 1 OS=Gallus gallus OX=9031 GN=SKP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H0511.6e-8096.91SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458404 PE=3 SV=1[more]
A0A6J1JGP31.5e-7090.26SKP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111486777 PE=3 SV=1[more]
A0A5A7SU156.9e-4761.44SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G... [more]
A0A5D3CCN66.9e-4761.44SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475... [more]
A0A1S3BGP66.9e-4761.44SKP1-like protein OS=Cucumis melo OX=3656 GN=LOC103489446 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G34210.11.9e-2847.95SKP1-like 11 [more]
AT2G03170.19.2e-2843.36SKP1-like 14 [more]
AT1G20140.13.5e-2740.65SKP1-like 4 [more]
AT4G34470.14.6e-2746.58SKP1-like 12 [more]
AT5G42190.14.6e-2739.64E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001232S-phase kinase-associated protein 1-likeSMARTSM00512skp1_3coord: 1..101
e-value: 9.3E-31
score: 118.2
IPR016897S-phase kinase-associated protein 1PIRSFPIRSF028729SCF_Skpcoord: 1..152
e-value: 1.1E-49
score: 166.3
IPR016897S-phase kinase-associated protein 1PANTHERPTHR11165SKP1coord: 3..148
IPR011333SKP1/BTB/POZ domain superfamilyGENE3D3.30.710.10Potassium Channel Kv1.1; Chain Acoord: 1..150
e-value: 1.7E-51
score: 176.5
IPR011333SKP1/BTB/POZ domain superfamilySUPERFAMILY54695POZ domaincoord: 3..67
IPR016072SKP1 component, dimerisationPFAMPF01466Skp1coord: 103..148
e-value: 5.0E-22
score: 77.7
IPR016073SKP1 component, POZ domainPFAMPF03931Skp1_POZcoord: 4..64
e-value: 5.1E-19
score: 68.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..162
NoneNo IPR availableCDDcd18322BTB_POZ_SKP1coord: 3..116
e-value: 3.43107E-38
score: 124.627
IPR036296SKP1-like, dimerisation domain superfamilySUPERFAMILY81382Skp1 dimerisation domain-likecoord: 74..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01943-RACarg01943-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0016567 protein ubiquitination
biological_process GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0097602 cullin family protein binding
molecular_function GO:0016301 kinase activity