Carg01840 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01840
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionHomocysteine S-methyltransferase family protein
LocationCarg_Chr04: 9051093 .. 9052739 (-)
RNA-Seq ExpressionCarg01840
SyntenyCarg01840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATCCCTGGACATAATTACAATCGGCTCTGGTTGCAGCATCCATTGGAAGCCATGGTGCTTATCTGCTGATGGCTCAGAGTACAGGTGAATTATACATTTGAATATTGAAATAAATATGACTCTGACATTCTGAACTAAACATATCCCCCCCTCTAGTGGGCATTACGGGCCAGATGTGAATGTGGATAAGTTGAAGGATTTTCATCGGCGCAGATTGCAGATTCTTGTTGATGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGTCTGGTTTATGTCAACTCCTCTTATATCATCTAAGTTGTGGTTTAATGATAGAATCACTCTTGCTGCCACAGTGATACCACTGTAAATTATATTGTTATGTTTTGGTATGAGTTATAATACAGTATTTGAGAAAACAGCCTATGGTATATTTTATAAACTGTTCATCTCAATTTATGAGTTGTTCATTTCAAGGGATCAAGGTAATTTGACTACCATCATGGCATCAATGGGGGATGCATGCATCAGTAGGTCTTAGTTTATTGTGCTTAATTCATGATCAAAATTCTTTTATAATAAAAATTTGAATTAAATCCTTATTCAAACCCCTTAATTTAAGATTTTTCCCATTTTACAAGTTCAGATATTATATTTTAATTCCTAAACCAGAATCCTGATTTAGTTGGTCTTTGTTATGAGATGGAAGAAAAATGTTGAATGTTTTTGATAATTGTCATGAGCATGTCGGACAATCGTTTCAATTGATTTTGCAATTTGTTTGGGTTTCTGTCCTCTTTGTGATCTTGTTTCCAACCAATCTTACCTTGGCTCCCCCAACTCTGGTCTTAAAAAAAAAAAAAAAAAAAAAAGTAGGCCTGTGTGGAGTTGCTCGAAGAAGAAAACGTCCAAATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGCGAGAATGCACCGTCGGGAGAGAGCTTCGAGAAGTGCCTTCATGCCATTAATAAAAGTGACAAAGTAACTGCAGTTGGCATAAACTGTACACCTCCTCACTTTATTGAAGCTCTGATTTGTAAGTTCAAAGAGGTAGGAACTAAATCTCTTAGTGTTGATTTAATATTGTTGTGTTGGTCACTTGTTTTGAAACCGAAATGTTCACTTCAGTTAACGAACAAGCACATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGCAGAGCCAAGAAATGGCTGGTGAGTCAACTTAATTCTTCCCTACCCCACTCTGATATTTCATGGCTTGTGGGCCTACATTGTAATATTTCCATTACTAATTAGCGTTCTGGTTTTGGTAGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCATTTTAGTTCAAGATGGCGTGATTTGGGAGCAAAATTCATTGGAGGCTGTTGTCCTACGACACCGTCTACCATCCTAGCTATATCAAAGGTTTGGAAAGAGAGCTCCCATTAGCGACCGCCGTCTAATTTTTGAACTTTTTGTTCTTTTGTAAACTACTAGATTGAGGTGTTATGGTTTGCATTCTGACATAATTTTTTCAGCTTATAGAACAATAGGCTAGTTAAAGAGGGTAGGCATTTTTACTTCTTCATGAAATTCAACTATTACATTT

mRNA sequence

ATGGTATCCCTGGACATAATTACAATCGGCTCTGGTTGCAGCATCCATTGGAAGCCATGGTGCTTATCTGCTGATGGCTCAGAGTACAGTGGGCATTACGGGCCAGATGTGAATGTGGATAAGTTGAAGGATTTTCATCGGCGCAGATTGCAGATTCTTGTTGATGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGCCTGTGTGGAGTTGCTCGAAGAAGAAAACGTCCAAATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGCGAGAATGCACCGTCGGGAGAGAGCTTCGAGAAGTGCCTTCATGCCATTAATAAAAGTGACAAAGTAACTGCAGTTGGCATAAACTGTACACCTCCTCACTTTATTGAAGCTCTGATTTGTAAGTTCAAAGAGTTAACGAACAAGCACATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGCAGAGCCAAGAAATGGCTGGTGAGTCAACTTAATTCTTCCCTACCCCACTCTGATATTTCATGGCTTGTGGGCCTACATTTTCAAGATGGCGTGATTTGGGAGCAAAATTCATTGGAGGCTGTTGTCCTACGACACCGTCTACCATCCTAGCTATATCAAAGGTTTGGAAAGAGAGCTCCCATTAGCGACCGCCGTCTAATTTTTGAACTTTTTGTTCTTTTGTAAACTACTAGATTGAGGTGTTATGGTTTGCATTCTGACATAATTTTTTCAGCTTATAGAACAATAGGCTAGTTAAAGAGGGTAGGCATTTTTACTTCTTCATGAAATTCAACTATTACATTT

Coding sequence (CDS)

ATGGTATCCCTGGACATAATTACAATCGGCTCTGGTTGCAGCATCCATTGGAAGCCATGGTGCTTATCTGCTGATGGCTCAGAGTACAGTGGGCATTACGGGCCAGATGTGAATGTGGATAAGTTGAAGGATTTTCATCGGCGCAGATTGCAGATTCTTGTTGATGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGCCTGTGTGGAGTTGCTCGAAGAAGAAAACGTCCAAATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGCGAGAATGCACCGTCGGGAGAGAGCTTCGAGAAGTGCCTTCATGCCATTAATAAAAGTGACAAAGTAACTGCAGTTGGCATAAACTGTACACCTCCTCACTTTATTGAAGCTCTGATTTGTAAGTTCAAAGAGTTAACGAACAAGCACATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGCAGAGCCAAGAAATGGCTGGTGAGTCAACTTAATTCTTCCCTACCCCACTCTGATATTTCATGGCTTGTGGGCCTACATTTTCAAGATGGCGTGATTTGGGAGCAAAATTCATTGGAGGCTGTTGTCCTACGACACCGTCTACCATCCTAG

Protein sequence

MVSLDIITIGSGCSIHWKPWCLSADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELTNKHIVVYPNSGEVWDGRAKKWLVSQLNSSLPHSDISWLVGLHFQDGVIWEQNSLEAVVLRHRLPS
Homology
[Close]
BLAST of Carg01840 vs. NCBI nr
Match: KAG7032078.1 (Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443.7 bits (1140), Expect = 8.5e-121
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0 

Query: 1   MVSLDIITIGSGCSIHWKPWCLSADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDL 60
           MVSLDIITIGSGCSIHWKPWCLSADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDL
Sbjct: 1   MVSLDIITIGSGCSIHWKPWCLSADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDL 60

Query: 61  LAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVT 120
           LAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVT
Sbjct: 61  LAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVT 120

Query: 121 AVGINCTPPHFIEALICKFKELTNKHIVVYPNSGEVWDGRAKKWLVSQLNSSLPHSDISW 180
           AVGINCTPPHFIEALICKFKELTNKHIVVYPNSGEVWDGRAKKWLVSQLNSSLPHSDISW
Sbjct: 121 AVGINCTPPHFIEALICKFKELTNKHIVVYPNSGEVWDGRAKKWLVSQLNSSLPHSDISW 180

Query: 181 LVGLHFQDGVIWEQNSLEAVVLRHRLPS 209
           LVGLHFQDGVIWEQNSLEAVVLRHRLPS
Sbjct: 181 LVGLHFQDGVIWEQNSLEAVVLRHRLPS 208

[Close]
BLAST of Carg01840 vs. NCBI nr
Match: KAG6601290.1 (Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 351.7 bits (901), Expect = 4.4e-93
Identity = 165/167 (98.80%), Postives = 165/167 (98.80%), Query Frame = 0 

Query: 1   MVSLDIITIGSGCSIHWKPWCLSADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDL 60
           MVSLDIITIGSGCSIHWK WCLSADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDL
Sbjct: 1   MVSLDIITIGSGCSIHWKLWCLSADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDL 60

Query: 61  LAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVT 120
           LAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVT
Sbjct: 61  LAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVT 120

Query: 121 AVGINCTPPHFIEALICKFKELTNKHIVVYPNSGEVWDGRAKKWLVS 168
           AVGINCTPPHFIEALICKFKELTNKHIVVYPNSGEVWDGRAKKWL S
Sbjct: 121 AVGINCTPPHFIEALICKFKELTNKHIVVYPNSGEVWDGRAKKWLPS 167

[Close]
BLAST of Carg01840 vs. NCBI nr
Match: XP_038893227.1 (homocysteine S-methyltransferase 1 [Benincasa hispida])

HSP 1 Score: 296.6 bits (758), Expect = 1.7e-76
Identity = 138/144 (95.83%), Postives = 141/144 (97.92%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN
Sbjct: 139 ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           +QIPSWICFSS+DGENAPSGESFEKCLHAINKSDKV AVGINCTPPHFIEALI KFKELT
Sbjct: 199 IQIPSWICFSSIDGENAPSGESFEKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVS 168
           NKHIVVYPNSGEVWDGR+KKWL S
Sbjct: 259 NKHIVVYPNSGEVWDGRSKKWLPS 282

[Close]
BLAST of Carg01840 vs. NCBI nr
Match: XP_008446599.1 (PREDICTED: homocysteine S-methyltransferase 1 [Cucumis melo])

HSP 1 Score: 292.4 bits (747), Expect = 3.2e-75
Identity = 138/144 (95.83%), Postives = 140/144 (97.22%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYGPDVNVDKLKDFHRRRL+ILVDASPDLLAFETIPNKLEAQACVELLEEEN
Sbjct: 139 ADGSEYSGHYGPDVNVDKLKDFHRRRLRILVDASPDLLAFETIPNKLEAQACVELLEEEN 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQIPSWICFSSVDGENAPSGESFEKCL+AINKSDKV AVGINCTPPHFIEALI KFKELT
Sbjct: 199 VQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVS 168
           NKHIVVYPNSGEVWDGR KKWL S
Sbjct: 259 NKHIVVYPNSGEVWDGRFKKWLPS 282

[Close]
BLAST of Carg01840 vs. NCBI nr
Match: XP_004135092.1 (homocysteine S-methyltransferase 1 isoform X1 [Cucumis sativus] >KGN52080.1 hypothetical protein Csa_008676 [Cucumis sativus])

HSP 1 Score: 292.0 bits (746), Expect = 4.2e-75
Identity = 137/144 (95.14%), Postives = 139/144 (96.53%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYGPDVNVDKLKDFHRRRLQI VDASPDLLAFETIPNKLEAQACVELLEEEN
Sbjct: 139 ADGSEYSGHYGPDVNVDKLKDFHRRRLQIFVDASPDLLAFETIPNKLEAQACVELLEEEN 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           +QIPSWICFSSVDGENAPSGESFEKCL+AINKSDKV AVGINCTPPHFIEALI KFKELT
Sbjct: 199 IQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVS 168
           NKHIVVYPNSGEVWDGR KKWL S
Sbjct: 259 NKHIVVYPNSGEVWDGRFKKWLPS 282

[Close]
BLAST of Carg01840 vs. ExPASy Swiss-Prot
Match: A4ZGQ8 (Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN=HMT1 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 6.3e-66
Identity = 115/145 (79.31%), Postives = 131/145 (90.34%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSG YG DV++DKLKDFHRRR+Q+LV+ASPDLLAFETIPNKLEAQACVELLEEEN
Sbjct: 139 ADGSEYSGSYGEDVSLDKLKDFHRRRIQVLVEASPDLLAFETIPNKLEAQACVELLEEEN 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQIP+WICF+SVDGENAPSGESF++CL  +NKS+ + AVGINC PP F++ LI KF +LT
Sbjct: 199 VQIPAWICFTSVDGENAPSGESFQECLETLNKSNNICAVGINCAPPQFMDNLIRKFSKLT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVSQ 169
            K IVVYPNSGEVWDG+AKKWL SQ
Sbjct: 259 QKAIVVYPNSGEVWDGKAKKWLPSQ 283

[Close]
BLAST of Carg01840 vs. ExPASy Swiss-Prot
Match: Q9SDL7 (Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 5.3e-65
Identity = 115/145 (79.31%), Postives = 129/145 (88.97%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+A PDLLAFETIPNKLEAQACVELLEEE 
Sbjct: 139 ADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVELLEEEK 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQIP+WICF+SVDGE APSGESFE+CL  +NKS+ + AVGINC PP FIE LI KF +LT
Sbjct: 199 VQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGINCAPPQFIENLIRKFAKLT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVSQ 169
            K IVVYPNSGEVWDG+AK+WL SQ
Sbjct: 259 KKAIVVYPNSGEVWDGKAKQWLPSQ 283

[Close]
BLAST of Carg01840 vs. ExPASy Swiss-Prot
Match: Q9FUN0 (Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.6e-58
Identity = 102/142 (71.83%), Postives = 117/142 (82.39%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSG YG D+  +KLKDFHRRRLQ+L  A PDL+AFE IPN++EAQA VELLEEE 
Sbjct: 133 ADGSEYSGSYGADITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNQMEAQALVELLEEEK 192

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQIPSWICFSSVDG+N  SGESF  CL  +N S+KV  VG+NCTPP FIE +IC+F++ T
Sbjct: 193 VQIPSWICFSSVDGKNLCSGESFADCLKILNASEKVAVVGVNCTPPQFIEGIICEFRKQT 252

Query: 144 NKHIVVYPNSGEVWDGRAKKWL 166
            K I VYPNSGEVWDGRAK+WL
Sbjct: 253 KKAIAVYPNSGEVWDGRAKRWL 274

[Close]
BLAST of Carg01840 vs. ExPASy Swiss-Prot
Match: Q9FUM8 (Homocysteine S-methyltransferase 3 OS=Zea mays OX=4577 GN=HMT-3 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 2.7e-45
Identity = 87/144 (60.42%), Postives = 107/144 (74.31%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSG YG     + LKDFHRRRLQ+L +A PDL+AFETIPNKLEAQA VELLEE N
Sbjct: 142 ADGSEYSGDYGEAGTKEFLKDFHRRRLQVLAEAGPDLIAFETIPNKLEAQAYVELLEECN 201

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           + IPSW+ F+S DG +  SG+S  +C    +K  KV AVGINCTPP FI  LI   +++T
Sbjct: 202 INIPSWLSFNSKDGVHVVSGDSLIECATIADKCAKVGAVGINCTPPRFIHGLILSIRKVT 261

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVS 168
           +K I++YPNSGE +DG  K+W+ S
Sbjct: 262 DKPILIYPNSGERYDGEKKEWVES 285

[Close]
BLAST of Carg01840 vs. ExPASy Swiss-Prot
Match: Q9M1W4 (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 4.7e-45
Identity = 81/142 (57.04%), Postives = 108/142 (76.06%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSG YG  + ++KLKDFHRRRLQ+L ++  DL+AFETIPNK+EAQA  +LLEE +
Sbjct: 143 ADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNKIEAQAFADLLEEGD 202

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           V+IP W  F+S DG N  SG+S ++C+      +KV AVGINCTPP FIE L+ + +++T
Sbjct: 203 VKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPPRFIEGLVLEIEKVT 262

Query: 144 NKHIVVYPNSGEVWDGRAKKWL 166
           +K I+VYPNSGE +D   K+W+
Sbjct: 263 SKPILVYPNSGESYDADRKEWV 284

[Close]
BLAST of Carg01840 vs. ExPASy TrEMBL
Match: A0A1S3BEY1 (homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.5e-75
Identity = 138/144 (95.83%), Postives = 140/144 (97.22%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYGPDVNVDKLKDFHRRRL+ILVDASPDLLAFETIPNKLEAQACVELLEEEN
Sbjct: 139 ADGSEYSGHYGPDVNVDKLKDFHRRRLRILVDASPDLLAFETIPNKLEAQACVELLEEEN 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQIPSWICFSSVDGENAPSGESFEKCL+AINKSDKV AVGINCTPPHFIEALI KFKELT
Sbjct: 199 VQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVS 168
           NKHIVVYPNSGEVWDGR KKWL S
Sbjct: 259 NKHIVVYPNSGEVWDGRFKKWLPS 282

[Close]
BLAST of Carg01840 vs. ExPASy TrEMBL
Match: A0A0A0KR42 (Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 2.0e-75
Identity = 137/144 (95.14%), Postives = 139/144 (96.53%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYGPDVNVDKLKDFHRRRLQI VDASPDLLAFETIPNKLEAQACVELLEEEN
Sbjct: 139 ADGSEYSGHYGPDVNVDKLKDFHRRRLQIFVDASPDLLAFETIPNKLEAQACVELLEEEN 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           +QIPSWICFSSVDGENAPSGESFEKCL+AINKSDKV AVGINCTPPHFIEALI KFKELT
Sbjct: 199 IQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVS 168
           NKHIVVYPNSGEVWDGR KKWL S
Sbjct: 259 NKHIVVYPNSGEVWDGRFKKWLPS 282

[Close]
BLAST of Carg01840 vs. ExPASy TrEMBL
Match: A0A5D3CFM3 (Homocysteine S-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00990 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 6.5e-74
Identity = 141/173 (81.50%), Postives = 150/173 (86.71%), Query Frame = 0 

Query: 38  NVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDG 97
           + DKLKDFHRRRL+ILVDASPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDG
Sbjct: 135 STDKLKDFHRRRLRILVDASPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDG 194

Query: 98  ENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELTNKHIVVYPNSGEVW 157
           ENAPSGESFEKCL+AINKSDKV AVGINCTPPHFIEALI KFKELTNKHIVVYPNSGEVW
Sbjct: 195 ENAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVW 254

Query: 158 DGRAKKWLVSQLNSSLPHSDISWLVGLH--FQDGVIWEQNSLEAVVLRHRLPS 209
           DGR KKWL S   S   H  +  +   +   QDG  WEQ+SLEAVV++HRLPS
Sbjct: 255 DGRFKKWLCSGFGS---HQIVLVMTNSNHLLQDGANWEQHSLEAVVVQHRLPS 304

[Close]
BLAST of Carg01840 vs. ExPASy TrEMBL
Match: A0A6J1D951 (homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111018744 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 2.7e-72
Identity = 132/144 (91.67%), Postives = 139/144 (96.53%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSG+YGPDVNVDKLKDFHRRRLQ+LV+A PDLLAFETIPNKLEAQACVELLEEEN
Sbjct: 139 ADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFETIPNKLEAQACVELLEEEN 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQIPSWICFSSVDG+NAPSGESFE+CL AINKS+KV AVGINCTPPHFIEALICKFKELT
Sbjct: 199 VQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGINCTPPHFIEALICKFKELT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVS 168
           +K IVVYPNSGEVWDGRAKKWL S
Sbjct: 259 DKPIVVYPNSGEVWDGRAKKWLPS 282

[Close]
BLAST of Carg01840 vs. ExPASy TrEMBL
Match: A0A6J1HZT4 (homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467714 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 3.6e-72
Identity = 134/144 (93.06%), Postives = 137/144 (95.14%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYGPDV+VD LKDFHRRRLQILV ASPDLLAFETIPNKLEAQACVELLEEEN
Sbjct: 139 ADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFETIPNKLEAQACVELLEEEN 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQI SWICFSSVDGENAPSGESFE+CL AINKSDKV AVGINCTPPHFIEALICKFKELT
Sbjct: 199 VQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGINCTPPHFIEALICKFKELT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVS 168
           +K IVVYPNSGEVWDGRAKKWL S
Sbjct: 259 DKPIVVYPNSGEVWDGRAKKWLPS 282

[Close]
BLAST of Carg01840 vs. TAIR 10
Match: AT3G25900.1 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 248.8 bits (634), Expect = 3.8e-66
Identity = 115/145 (79.31%), Postives = 129/145 (88.97%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+A PDLLAFETIPNKLEAQACVELLEEE 
Sbjct: 139 ADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVELLEEEK 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQIP+WICF+SVDGE APSGESFE+CL  +NKS+ + AVGINC PP FIE LI KF +LT
Sbjct: 199 VQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGINCAPPQFIENLIRKFAKLT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVSQ 169
            K IVVYPNSGEVWDG+AK+WL SQ
Sbjct: 259 KKAIVVYPNSGEVWDGKAKQWLPSQ 283

[Close]
BLAST of Carg01840 vs. TAIR 10
Match: AT3G25900.3 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 208.0 bits (528), Expect = 7.4e-54
Identity = 101/145 (69.66%), Postives = 114/145 (78.62%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+A PDLLAFETIPNKLEAQACVELLEEE 
Sbjct: 139 ADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVELLEEEK 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQIP+WICF+SVDGE APSGESFE+CL  +NKS+ + A                    LT
Sbjct: 199 VQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYA--------------------LT 258

Query: 144 NKHIVVYPNSGEVWDGRAKKWLVSQ 169
            K IVVYPNSGEVWDG+AK+WL SQ
Sbjct: 259 KKAIVVYPNSGEVWDGKAKQWLPSQ 263

[Close]
BLAST of Carg01840 vs. TAIR 10
Match: AT3G25900.2 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 203.4 bits (516), Expect = 1.8e-52
Identity = 94/122 (77.05%), Postives = 108/122 (88.52%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+A PDLLAFETIPNKLEAQACVELLEEE 
Sbjct: 139 ADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVELLEEEK 198

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           VQIP+WICF+SVDGE APSGESFE+CL  +NKS+ + AVGINC PP FIE LI KF + T
Sbjct: 199 VQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGINCAPPQFIENLIRKFAKAT 258

Query: 144 NK 146
           ++
Sbjct: 259 DE 260

[Close]
BLAST of Carg01840 vs. TAIR 10
Match: AT3G63250.2 (homocysteine methyltransferase 2 )

HSP 1 Score: 182.6 bits (462), Expect = 3.3e-46
Identity = 81/142 (57.04%), Postives = 108/142 (76.06%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSG YG  + ++KLKDFHRRRLQ+L ++  DL+AFETIPNK+EAQA  +LLEE +
Sbjct: 103 ADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNKIEAQAFADLLEEGD 162

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           V+IP W  F+S DG N  SG+S ++C+      +KV AVGINCTPP FIE L+ + +++T
Sbjct: 163 VKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPPRFIEGLVLEIEKVT 222

Query: 144 NKHIVVYPNSGEVWDGRAKKWL 166
           +K I+VYPNSGE +D   K+W+
Sbjct: 223 SKPILVYPNSGESYDADRKEWV 244

[Close]
BLAST of Carg01840 vs. TAIR 10
Match: AT3G63250.1 (homocysteine methyltransferase 2 )

HSP 1 Score: 182.6 bits (462), Expect = 3.3e-46
Identity = 81/142 (57.04%), Postives = 108/142 (76.06%), Query Frame = 0 

Query: 24  ADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEEN 83
           ADGSEYSG YG  + ++KLKDFHRRRLQ+L ++  DL+AFETIPNK+EAQA  +LLEE +
Sbjct: 143 ADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNKIEAQAFADLLEEGD 202

Query: 84  VQIPSWICFSSVDGENAPSGESFEKCLHAINKSDKVTAVGINCTPPHFIEALICKFKELT 143
           V+IP W  F+S DG N  SG+S ++C+      +KV AVGINCTPP FIE L+ + +++T
Sbjct: 203 VKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPPRFIEGLVLEIEKVT 262

Query: 144 NKHIVVYPNSGEVWDGRAKKWL 166
           +K I+VYPNSGE +D   K+W+
Sbjct: 263 SKPILVYPNSGESYDADRKEWV 284

The following BLAST results are available for this feature:
BLAST of Carg01840 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7032078.18.5e-121100.00Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. argyr... [more]
KAG6601290.14.4e-9398.80Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_038893227.11.7e-7695.83homocysteine S-methyltransferase 1 [Benincasa hispida][more]
XP_008446599.13.2e-7595.83PREDICTED: homocysteine S-methyltransferase 1 [Cucumis melo][more]
XP_004135092.14.2e-7595.14homocysteine S-methyltransferase 1 isoform X1 [Cucumis sativus] >KGN52080.1 hypo... [more]
BLAST of Carg01840 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A4ZGQ86.3e-6679.31Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN... [more]
Q9SDL75.3e-6579.31Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1... [more]
Q9FUN01.6e-5871.83Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1[more]
Q9FUM82.7e-4560.42Homocysteine S-methyltransferase 3 OS=Zea mays OX=4577 GN=HMT-3 PE=2 SV=1[more]
Q9M1W44.7e-4557.04Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1... [more]
BLAST of Carg01840 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BEY11.5e-7595.83homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 ... [more]
A0A0A0KR422.0e-7595.14Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660... [more]
A0A5D3CFM36.5e-7481.50Homocysteine S-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1D9512.7e-7291.67homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC11101874... [more]
A0A6J1HZT43.6e-7293.06homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467... [more]
BLAST of Carg01840 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G25900.13.8e-6679.31Homocysteine S-methyltransferase family protein [more]
AT3G25900.37.4e-5469.66Homocysteine S-methyltransferase family protein [more]
AT3G25900.21.8e-5277.05Homocysteine S-methyltransferase family protein [more]
AT3G63250.23.3e-4657.04homocysteine methyltransferase 2 [more]
AT3G63250.13.3e-4657.04homocysteine methyltransferase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003726Homocysteine-binding domainPFAMPF02574S-methyl_transcoord: 31..166
e-value: 1.9E-32
score: 113.3
IPR003726Homocysteine-binding domainPROSITEPS50970HCYcoord: 1..208
score: 14.047519
IPR036589Homocysteine-binding domain superfamilyGENE3D3.20.20.330coord: 19..188
e-value: 1.2E-41
score: 145.0
IPR036589Homocysteine-binding domain superfamilySUPERFAMILY82282Homocysteine S-methyltransferasecoord: 27..165
NoneNo IPR availablePANTHERPTHR46015:SF7HOMOCYSTEINE S-METHYLTRANSFERASE 1coord: 24..168
NoneNo IPR availablePANTHERPTHR46015ZGC:172121coord: 24..168

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01840-RACarg01840-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0032259 methylation
biological_process GO:0033528 S-methylmethionine cycle
molecular_function GO:0047150 betaine-homocysteine S-methyltransferase activity
molecular_function GO:0008898 S-adenosylmethionine-homocysteine S-methyltransferase activity
molecular_function GO:0008270 zinc ion binding