Carg01827 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01827
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Aspartyl protease family protein
Location	Carg_Chr04: 9122249 .. 9123706 (+)
RNA-Seq Expression	Carg01827
Synteny	Carg01827

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACCGCCTTTTTTCTCTTCCTTCTTCCCCTTCTTTACGCCTCTTTTTCCGGCGTTCTCTCCCGGACCTTGCCTCACGCCCCTCGGACCTCCGTGCTCGATGTCGACGCTTCCATTCACACCACTCGCCAGGTCTTTGCATTTCAGCCTCAATCTCCAATGCGAGATGAAAGTACCGTCTCGGACTCTTCTTCCTTGACTCTGCTGTTGAATTCCAGGGTTTCCATTATGAAAACCTCGCACATTGACTACAAATCCCTCACGTTATCCAGACTCTACCGCGACTCTGCTCGAGTCAGATCTTTGACTGCAAGGATGGATCTAGCCATTCGAGGTATTACTGGAGCGGATCTTGAACCTCTCTGGAATGGTGGTGGTTCGCAATTTGGGGCGGAGGATTTTGAGAGTCCGATTGTCTCAGGCGCGAGTCAGGGGAGCGGTGAGTACTTCTCCCGAGTTGGAATCGGTAGGCCGCCGAGTCCGGTTTACATGGTGCTCGATACTGGTAGTGATGTAAGTTGGGTACAATGCGCGCCTTGTGCTGATTGCTACGAGCAAACCGATCCAATTTTTGAGCCTACTTCCTCTGCTTCTTTCACGTCTCTGTCCTGCCAAACACAGCAATGTAAATCGCTCGATGTTTCTGAGTGCCGGAATGGTACTTGTCTCTACGAGGTCTCTTATGGCGATGGTTCTTACACCGTCGGCGATTTCGTTACTGAAACTGTTACTCTCGGCTCGACTTCTCTCACAAATATCGCTCTAGGCTGTGGCCATAACAATGAGGGTTTGTTCATCGGCGCCGCCGGTTTGCTCGGACTAGGAGGCGGCTCGCTCTCGTTCCCTTCGCAGCTTAATGCCTCGTCTTTTTCGTACTGTCTTGTGGACCGTGACTCTGAATCCACCTCGACTCTCGATTTCAACTCACCGATTCCTCCCGATGCCGTAACAGCGCCGCTGCACCGGAACCCTAATTTGGACACGTTTTTCTACCTCGGCATGACAGGGATGAGCGTCGGAGGTGAAATTCTTCCGATTCCCGAGACGTCGTTCCAAATGAGCCAAGACGGAAACGGCGGCATCATCATTGACTCCGGCACCGCCGTGACGCGGTTGCAGACCACCGCTTATAACTTGTTGCGCGACGCGTTCGTTAAGAAGACGCACGATTTGCAGTCCGCACGTGGCGTGGCGTTGTTTGATACTTGTTACGACTTGTCGTCGAAGTCGAGAGTCGAAGTGCCGACGGTGTCATTTCACTTCGCGGACGGGAAAGAGTTGCCGTTGCCGGCAAAAAATTACCTGATACCGGTTGACTCGGAAGGAACATTTTGTTTCGCCTTTGCCCCTACCGATTCAACATTGTCAATACTCGGAAACGCACAGCAGCAAGGGACACGTGTCAGTTTCGACCTCGCTAATTCGCTCGTTGGGTTCTCCTCCAACAAATGCTAA

mRNA sequence

ATGGCCACCGCCTTTTTTCTCTTCCTTCTTCCCCTTCTTTACGCCTCTTTTTCCGGCGTTCTCTCCCGGACCTTGCCTCACGCCCCTCGGACCTCCGTGCTCGATGTCGACGCTTCCATTCACACCACTCGCCAGGTCTTTGCATTTCAGCCTCAATCTCCAATGCGAGATGAAAGTACCGTCTCGGACTCTTCTTCCTTGACTCTGCTGTTGAATTCCAGGGTTTCCATTATGAAAACCTCGCACATTGACTACAAATCCCTCACGTTATCCAGACTCTACCGCGACTCTGCTCGAGTCAGATCTTTGACTGCAAGGATGGATCTAGCCATTCGAGGTATTACTGGAGCGGATCTTGAACCTCTCTGGAATGGTGGTGGTTCGCAATTTGGGGCGGAGGATTTTGAGAGTCCGATTGTCTCAGGCGCGAGTCAGGGGAGCGGTGAGTACTTCTCCCGAGTTGGAATCGGTAGGCCGCCGAGTCCGGTTTACATGGTGCTCGATACTGGTAGTGATGTAAGTTGGGTACAATGCGCGCCTTGTGCTGATTGCTACGAGCAAACCGATCCAATTTTTGAGCCTACTTCCTCTGCTTCTTTCACGTCTCTGTCCTGCCAAACACAGCAATGTAAATCGCTCGATGTTTCTGAGTGCCGGAATGGTACTTGTCTCTACGAGGTCTCTTATGGCGATGGTTCTTACACCGTCGGCGATTTCGTTACTGAAACTGTTACTCTCGGCTCGACTTCTCTCACAAATATCGCTCTAGGCTGTGGCCATAACAATGAGGGTTTGTTCATCGGCGCCGCCGGTTTGCTCGGACTAGGAGGCGGCTCGCTCTCGTTCCCTTCGCAGCTTAATGCCTCGTCTTTTTCGTACTGTCTTGTGGACCGTGACTCTGAATCCACCTCGACTCTCGATTTCAACTCACCGATTCCTCCCGATGCCGTAACAGCGCCGCTGCACCGGAACCCTAATTTGGACACGTTTTTCTACCTCGGCATGACAGGGATGAGCGTCGGAGGTGAAATTCTTCCGATTCCCGAGACGTCGTTCCAAATGAGCCAAGACGGAAACGGCGGCATCATCATTGACTCCGGCACCGCCGTGACGCGGTTGCAGACCACCGCTTATAACTTGTTGCGCGACGCGTTCGTTAAGAAGACGCACGATTTGCAGTCCGCACGTGGCGTGGCGTTGTTTGATACTTGTTACGACTTGTCGTCGAAGTCGAGAGTCGAAGTGCCGACGGTGTCATTTCACTTCGCGGACGGGAAAGAGTTGCCGTTGCCGGCAAAAAATTACCTGATACCGGTTGACTCGGAAGGAACATTTTGTTTCGCCTTTGCCCCTACCGATTCAACATTGTCAATACTCGGAAACGCACAGCAGCAAGGGACACGTGTCAGTTTCGACCTCGCTAATTCGCTCGTTGGGTTCTCCTCCAACAAATGCTAA

Coding sequence (CDS)

ATGGCCACCGCCTTTTTTCTCTTCCTTCTTCCCCTTCTTTACGCCTCTTTTTCCGGCGTTCTCTCCCGGACCTTGCCTCACGCCCCTCGGACCTCCGTGCTCGATGTCGACGCTTCCATTCACACCACTCGCCAGGTCTTTGCATTTCAGCCTCAATCTCCAATGCGAGATGAAAGTACCGTCTCGGACTCTTCTTCCTTGACTCTGCTGTTGAATTCCAGGGTTTCCATTATGAAAACCTCGCACATTGACTACAAATCCCTCACGTTATCCAGACTCTACCGCGACTCTGCTCGAGTCAGATCTTTGACTGCAAGGATGGATCTAGCCATTCGAGGTATTACTGGAGCGGATCTTGAACCTCTCTGGAATGGTGGTGGTTCGCAATTTGGGGCGGAGGATTTTGAGAGTCCGATTGTCTCAGGCGCGAGTCAGGGGAGCGGTGAGTACTTCTCCCGAGTTGGAATCGGTAGGCCGCCGAGTCCGGTTTACATGGTGCTCGATACTGGTAGTGATGTAAGTTGGGTACAATGCGCGCCTTGTGCTGATTGCTACGAGCAAACCGATCCAATTTTTGAGCCTACTTCCTCTGCTTCTTTCACGTCTCTGTCCTGCCAAACACAGCAATGTAAATCGCTCGATGTTTCTGAGTGCCGGAATGGTACTTGTCTCTACGAGGTCTCTTATGGCGATGGTTCTTACACCGTCGGCGATTTCGTTACTGAAACTGTTACTCTCGGCTCGACTTCTCTCACAAATATCGCTCTAGGCTGTGGCCATAACAATGAGGGTTTGTTCATCGGCGCCGCCGGTTTGCTCGGACTAGGAGGCGGCTCGCTCTCGTTCCCTTCGCAGCTTAATGCCTCGTCTTTTTCGTACTGTCTTGTGGACCGTGACTCTGAATCCACCTCGACTCTCGATTTCAACTCACCGATTCCTCCCGATGCCGTAACAGCGCCGCTGCACCGGAACCCTAATTTGGACACGTTTTTCTACCTCGGCATGACAGGGATGAGCGTCGGAGGTGAAATTCTTCCGATTCCCGAGACGTCGTTCCAAATGAGCCAAGACGGAAACGGCGGCATCATCATTGACTCCGGCACCGCCGTGACGCGGTTGCAGACCACCGCTTATAACTTGTTGCGCGACGCGTTCGTTAAGAAGACGCACGATTTGCAGTCCGCACGTGGCGTGGCGTTGTTTGATACTTGTTACGACTTGTCGTCGAAGTCGAGAGTCGAAGTGCCGACGGTGTCATTTCACTTCGCGGACGGGAAAGAGTTGCCGTTGCCGGCAAAAAATTACCTGATACCGGTTGACTCGGAAGGAACATTTTGTTTCGCCTTTGCCCCTACCGATTCAACATTGTCAATACTCGGAAACGCACAGCAGCAAGGGACACGTGTCAGTTTCGACCTCGCTAATTCGCTCGTTGGGTTCTCCTCCAACAAATGCTAA

Protein sequence

MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDESTVSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLEPLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSSNKC

Homology

[Close]

BLAST of Carg01827 vs. NCBI nr
Match: KAG6601306.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032091.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 960.3 bits (2481), Expect = 6.3e-276
Identity = 485/485 (100.00%), Postives = 485/485 (100.00%), Query Frame = 0


Query: 1   MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60
           MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDEST
Sbjct: 1   MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120
           VSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE
Sbjct: 61  VSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120

Query: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 486
           SSNKC
Sbjct: 481 SSNKC 485

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BLAST of Carg01827 vs. NCBI nr
Match: XP_022957517.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata])

HSP 1 Score: 955.3 bits (2468), Expect = 2.0e-274
Identity = 482/485 (99.38%), Postives = 484/485 (99.79%), Query Frame = 0


Query: 1   MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60
           MATAFFLFLLPLLYASFSGVLSR+LPHAP+TSVLDVDASIHTTRQVFAFQPQSPMRDEST
Sbjct: 1   MATAFFLFLLPLLYASFSGVLSRSLPHAPQTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120
           VSDSSSLTLLLNSRVSIMKTSH DYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE
Sbjct: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120

Query: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 486
           SSNKC
Sbjct: 481 SSNKC 485

[Close]

BLAST of Carg01827 vs. NCBI nr
Match: XP_023544196.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936.0 bits (2418), Expect = 1.3e-268
Identity = 476/485 (98.14%), Postives = 478/485 (98.56%), Query Frame = 0


Query: 1   MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60
           MATAFFLFLLPLLYASFSGVLSRTLPHA  TSVLDV ASIHTTRQVFAFQPQSPMRDE  
Sbjct: 1   MATAFFLFLLPLLYASFSGVLSRTLPHASGTSVLDVAASIHTTRQVFAFQPQSPMRDE-- 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120
            SDSSSLTLLLNSRVSI+KTSH DYKSLTLSRL+RDSARVRSLTARMDLAIRGITGADLE
Sbjct: 61  -SDSSSLTLLLNSRVSIIKTSHTDYKSLTLSRLHRDSARVRSLTARMDLAIRGITGADLE 120

Query: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 486
           SSNKC
Sbjct: 481 SSNKC 482

[Close]

BLAST of Carg01827 vs. NCBI nr
Match: XP_022983595.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 927.9 bits (2397), Expect = 3.5e-266
Identity = 474/485 (97.73%), Postives = 475/485 (97.94%), Query Frame = 0


Query: 1   MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60
           MAT F LFLLPLLYASFS VLSRTLPHAP+TSVLDV ASIHTTRQVFAFQPQSPMRDEST
Sbjct: 1   MAT-FLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDEST 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120
           VSDSSSLTLLLNSRVSIMKTSH DYKSLTLSRLYRDSARVRSLTARMDLAI GITGADLE
Sbjct: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLE 120

Query: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PL  GG SQFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 486
           SSNKC
Sbjct: 481 SSNKC 484

[Close]

BLAST of Carg01827 vs. NCBI nr
Match: XP_038891764.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida])

HSP 1 Score: 849.7 bits (2194), Expect = 1.2e-242
Identity = 429/479 (89.56%), Postives = 450/479 (93.95%), Query Frame = 0


Query: 7   LFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDESTVSDSSS 66
           LFLLPLL++S SG L R+LP  P T+VLDV  SI  T+Q+FA  P+S M DE+TVSDSSS
Sbjct: 28  LFLLPLLFSSLSGFLCRSLPPTPTTAVLDVATSIQRTQQIFALDPESSMTDENTVSDSSS 87

Query: 67  LTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLEPLWNGG 126
           LTL L+SR+S+MKTSH DYKSLTLSRL RDSARVRSLTARMDLAIRGITGADLEP     
Sbjct: 88  LTLQLHSRISVMKTSHSDYKSLTLSRLKRDSARVRSLTARMDLAIRGITGADLEP----- 147

Query: 127 GSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCYE 186
             QFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCA+CYE
Sbjct: 148 --QFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCAECYE 207

Query: 187 QTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTL 246
           QTDPIFEPTSSASFTSLSC+T+QCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVT 
Sbjct: 208 QTDPIFEPTSSASFTSLSCETEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTF 267

Query: 247 GSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTSTL 306
           GSTSL NIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS+STSTL
Sbjct: 268 GSTSLRNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSDSTSTL 327

Query: 307 DFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGIIIDS 366
           DFNSPIPPDAVTAPLHRNPNL+TFFYLG+TGMSVGG+ILPIPE+SFQMSQDGNGGIIIDS
Sbjct: 328 DFNSPIPPDAVTAPLHRNPNLNTFFYLGVTGMSVGGDILPIPESSFQMSQDGNGGIIIDS 387

Query: 367 GTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHFADGK 426
           GTAVTRLQTTAYN+LRDAFVK THDLQSARGVALFDTCYDLSSKSRVEVPTVSFHFA+GK
Sbjct: 388 GTAVTRLQTTAYNVLRDAFVKSTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHFANGK 447

Query: 427 ELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSSNKC 486
           ELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRV FDLANSLVGFS NKC
Sbjct: 448 ELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSLVGFSPNKC 499

[Close]

BLAST of Carg01827 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.3e-151
Identity = 273/501 (54.49%), Postives = 364/501 (72.65%), Query Frame = 0


Query: 1   MATAFFLFLLPL----LYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQP----- 60
           MA   FL LL +    L+ + +   SR+L   P+T+VLDV +S+  T+ + +  P     
Sbjct: 1   MAFPRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSL 60

Query: 61  ----QSPMRDESTVSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARM 120
                  + D    + SS L+L L+SR + + + H DYKSLTLSRL RDS+RV  + A++
Sbjct: 61  TTTKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKI 120

Query: 121 DLAIRGITGADLEPLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVL 180
             A+ G+  +DL+P++N   +++  ED  +P+VSGASQGSGEYFSR+G+G P   +Y+VL
Sbjct: 121 RFAVEGVDRSDLKPVYN-EDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVL 180

Query: 181 DTGSDVSWVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEV 240
           DTGSDV+W+QC PCADCY+Q+DP+F PTSS+++ SL+C   QC  L+ S CR+  CLY+V
Sbjct: 181 DTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQV 240

Query: 241 SYGDGSYTVGDFVTETVTLG-STSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQL 300
           SYGDGS+TVG+  T+TVT G S  + N+ALGCGH+NEGLF GAAGLLGLGGG LS  +Q+
Sbjct: 241 SYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM 300

Query: 301 NASSFSYCLVDRDSESTSTLDFNS-PIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEIL 360
            A+SFSYCLVDRDS  +S+LDFNS  +     TAPL RN  +DTF+Y+G++G SVGGE +
Sbjct: 301 KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKV 360

Query: 361 PIPETSFQMSQDGNGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDL-QSARGVALFDTC 420
            +P+  F +   G+GG+I+D GTAVTRLQT AYN LRDAF+K T +L + +  ++LFDTC
Sbjct: 361 VLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTC 420

Query: 421 YDLSSKSRVEVPTVSFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQ 480
           YD SS S V+VPTV+FHF  GK L LPAKNYLIPVD  GTFCFAFAPT S+LSI+GN QQ
Sbjct: 421 YDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQ 480

Query: 481 QGTRVSFDLANSLVGFSSNKC 486
           QGTR+++DL+ +++G S NKC
Sbjct: 481 QGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Carg01827 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 3.9e-111
Identity = 220/485 (45.36%), Postives = 298/485 (61.44%), Query Frame = 0


Query: 5   FFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDESTVSDS 64
           FF FL   L+ S S  +S      P   ++DV     T          +   DES    S
Sbjct: 6   FFFFLHLHLHLSSSSSIS-----FPDFQIIDVLQPPLTVTATLPDFNNTHFSDES----S 65

Query: 65  SSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLEPLWN 124
           S  TL L  R      ++ ++     +R+ RD+ RV ++       +R I+G  +     
Sbjct: 66  SKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAI-------LRRISGKVIP---- 125

Query: 125 GGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADC 184
              S++   DF S IVSG  QGSGEYF R+G+G PP   YMV+D+GSD+ WVQC PC  C
Sbjct: 126 SSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC 185

Query: 185 YEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV 244
           Y+Q+DP+F+P  S S+T +SC +  C  ++ S C +G C YEV YGDGSYT G    ET+
Sbjct: 186 YKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETL 245

Query: 245 TLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLN---ASSFSYCLVDRDSE 304
           T   T + N+A+GCGH N G+FIGAAGLLG+GGGS+SF  QL+     +F YCLV R ++
Sbjct: 246 TFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTD 305

Query: 305 STSTLDF-NSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 364
           ST +L F    +P  A   PL RNP   +F+Y+G+ G+ VGG  +P+P+  F +++ G+G
Sbjct: 306 STGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDG 365

Query: 365 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 424
           G+++D+GTAVTRL T AY   RD F  +T +L  A GV++FDTCYDLS    V VPTVSF
Sbjct: 366 GVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSF 425

Query: 425 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 484
           +F +G  L LPA+N+L+PVD  GT+CFAFA + + LSI+GN QQ+G +VSFD AN  VGF
Sbjct: 426 YFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGF 470

Query: 485 SSNKC 486
             N C
Sbjct: 486 GPNVC 470

[Close]

BLAST of Carg01827 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.8e-108
Identity = 224/443 (50.56%), Postives = 285/443 (64.33%), Query Frame = 0


Query: 52  QSPMRDESTVSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSL-TARMDLA 111
           +S     S    SSS+TL L+   ++      D   L  SRL RDS RV+S+ T    + 
Sbjct: 57  ESEFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIP 116

Query: 112 IRGITGADLEPLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTG 171
            R +T A        GG       F S +VSG SQGSGEYF+R+G+G P   VYMVLDTG
Sbjct: 117 GRNVTHAP-----RPGG-------FSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTG 176

Query: 172 SDVSWVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSEC--RNGTCLYEVS 231
           SD+ W+QCAPC  CY Q+DPIF+P  S ++ ++ C +  C+ LD + C  R  TCLY+VS
Sbjct: 177 SDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVS 236

Query: 232 YGDGSYTVGDFVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLN- 291
           YGDGS+TVGDF TET+T     +  +ALGCGH+NEGLF+GAAGLLGLG G LSFP Q   
Sbjct: 237 YGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGH 296

Query: 292 --ASSFSYCLVDRDSES--TSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGE 351
                FSYCLVDR + S  +S +  N+ +   A   PL  NP LDTF+Y+G+ G+SVGG 
Sbjct: 297 RFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGT 356

Query: 352 ILP-IPETSFQMSQDGNGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFD 411
            +P +  + F++ Q GNGG+IIDSGT+VTRL   AY  +RDAF      L+ A   +LFD
Sbjct: 357 RVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFD 416

Query: 412 TCYDLSSKSRVEVPTVSFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNA 471
           TC+DLS+ + V+VPTV  HF  G ++ LPA NYLIPVD+ G FCFAFA T   LSI+GN 
Sbjct: 417 TCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNI 476

Query: 472 QQQGTRVSFDLANSLVGFSSNKC 486
           QQQG RV +DLA+S VGF+   C
Sbjct: 477 QQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Carg01827 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.4e-70
Identity = 144/355 (40.56%), Postives = 199/355 (56.06%), Query Frame = 0


Query: 137 SPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPTS 196
           S + +    G GEY   + IG P  P   ++DTGSD+ W QC PC  C+ Q+ PIF P  
Sbjct: 82  SGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQG 141

Query: 197 SASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSLTNIAL 256
           S+SF++L C +Q C++L    C N  C Y   YGDGS T G   TET+T GS S+ NI  
Sbjct: 142 SSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITF 201

Query: 257 GCGHNNEGLFIG-AAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTSTLDFNS---PI 316
           GCG NN+G   G  AGL+G+G G LS PSQL+ + FSYC+    S + S L   S    +
Sbjct: 202 GCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSV 261

Query: 317 PPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQM-SQDGNGGIIIDSGTAVT 376
              +    L ++  + TF+Y+ + G+SVG   LPI  ++F + S +G GGIIIDSGT +T
Sbjct: 262 TAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLT 321

Query: 377 RLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDL-SSKSRVEVPTVSFHFADGKELPL 436
                AY  +R  F+ + +        + FD C+   S  S +++PT   HF DG +L L
Sbjct: 322 YFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHF-DGGDLEL 381

Query: 437 PAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSSNKC 486
           P++NY I   S G  C A   +   +SI GN QQQ   V +D  NS+V F+S +C
Sbjct: 382 PSENYFIS-PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Carg01827 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.4e-68
Identity = 147/356 (41.29%), Postives = 198/356 (55.62%), Query Frame = 0


Query: 136 ESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCYEQTDPIFEPT 195
           E+P+ +    G GEY   V IG P S    ++DTGSD+ W QC PC  C+ Q  PIF P 
Sbjct: 86  ETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQ 145

Query: 196 SSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVTLGSTSLTNIA 255
            S+SF++L C++Q C+ L    C N  C Y   YGDGS T G   TET T  ++S+ NIA
Sbjct: 146 DSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIA 205

Query: 256 LGCGHNNEGLFIG-AAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTSTLDFNSP--- 315
            GCG +N+G   G  AGL+G+G G LS PSQL    FSYC+    S S STL   S    
Sbjct: 206 FGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASG 265

Query: 316 IPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGIIIDSGTAVT 375
           +P  + +  L  +    T++Y+ + G++VGG+ L IP ++FQ+  DG GG+IIDSGT +T
Sbjct: 266 VPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLT 325

Query: 376 RLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDL-SSKSRVEVPTVSFHFADGKELPL 435
            L   AYN +  AF  + +        +   TC+   S  S V+VP +S  F DG  L L
Sbjct: 326 YLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQF-DGGVLNL 385

Query: 436 PAKNYLIPVDSEGTFCFAFAPTDST-LSILGNAQQQGTRVSFDLANSLVGFSSNKC 486
             +N LI   +EG  C A   +    +SI GN QQQ T+V +DL N  V F   +C
Sbjct: 386 GEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Carg01827 vs. ExPASy TrEMBL
Match: A0A6J1GZC0 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458894 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 9.9e-275
Identity = 482/485 (99.38%), Postives = 484/485 (99.79%), Query Frame = 0


Query: 1   MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60
           MATAFFLFLLPLLYASFSGVLSR+LPHAP+TSVLDVDASIHTTRQVFAFQPQSPMRDEST
Sbjct: 1   MATAFFLFLLPLLYASFSGVLSRSLPHAPQTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120
           VSDSSSLTLLLNSRVSIMKTSH DYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE
Sbjct: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120

Query: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 486
           SSNKC
Sbjct: 481 SSNKC 485

[Close]

BLAST of Carg01827 vs. ExPASy TrEMBL
Match: A0A6J1J6A9 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482126 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.7e-266
Identity = 474/485 (97.73%), Postives = 475/485 (97.94%), Query Frame = 0


Query: 1   MATAFFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDEST 60
           MAT F LFLLPLLYASFS VLSRTLPHAP+TSVLDV ASIHTTRQVFAFQPQSPMRDEST
Sbjct: 1   MAT-FLLFLLPLLYASFSAVLSRTLPHAPQTSVLDVAASIHTTRQVFAFQPQSPMRDEST 60

Query: 61  VSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLE 120
           VSDSSSLTLLLNSRVSIMKTSH DYKSLTLSRLYRDSARVRSLTARMDLAI GITGADLE
Sbjct: 61  VSDSSSLTLLLNSRVSIMKTSHTDYKSLTLSRLYRDSARVRSLTARMDLAIPGITGADLE 120

Query: 121 PLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180
           PL  GG SQFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP
Sbjct: 121 PLPTGGASQFGMEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAP 180

Query: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240
           CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV
Sbjct: 181 CADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFV 240

Query: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300
           TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS
Sbjct: 241 TETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS 300

Query: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360
           ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG
Sbjct: 301 ESTSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 360

Query: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420
           GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF
Sbjct: 361 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 420

Query: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480
           HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF
Sbjct: 421 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 480

Query: 481 SSNKC 486
           SSNKC
Sbjct: 481 SSNKC 484

[Close]

BLAST of Carg01827 vs. ExPASy TrEMBL
Match: A0A1S3BFC3 (protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC103489261 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.9e-242
Identity = 426/481 (88.57%), Postives = 450/481 (93.56%), Query Frame = 0


Query: 7   LFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDESTVSDSSS 66
           LFLL LL++S S  L RTLP  PRTSVLDV ASI  T+Q+FA +P+S   DE TVSDSSS
Sbjct: 6   LFLLSLLFSSLSAFLCRTLPPTPRTSVLDVAASIQRTQQIFAMEPKSSTPDEITVSDSSS 65

Query: 67  LTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLEPLWN-- 126
           L+L LNSR+S+MKTSH DYKSLTLSRL RDSARV+SLTAR+DLAIRGITG DLEPL N  
Sbjct: 66  LSLQLNSRISVMKTSHSDYKSLTLSRLKRDSARVKSLTARIDLAIRGITGTDLEPLGNGD 125

Query: 127 GGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADC 186
           GGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCA+C
Sbjct: 126 GGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCAEC 185

Query: 187 YEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV 246
           YEQTDPIFEPTSSASFTSLSC+T+QCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV
Sbjct: 186 YEQTDPIFEPTSSASFTSLSCETEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV 245

Query: 247 TLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTS 306
           TLGSTSL NIA+GCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS+STS
Sbjct: 246 TLGSTSLRNIAIGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSDSTS 305

Query: 307 TLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGIII 366
           TLDFNSPI PDAVTAPLHRNPNLDTFFYLG+TGMSVGG +LPIPETSFQMS+DGNGGII+
Sbjct: 306 TLDFNSPISPDAVTAPLHRNPNLDTFFYLGLTGMSVGGTVLPIPETSFQMSEDGNGGIIV 365

Query: 367 DSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHFAD 426
           DSGTAVTRLQTT YN+LRD+FVK THDLQSARGVALFDTCYDLSSKS VEVPTVSFHFA+
Sbjct: 366 DSGTAVTRLQTTVYNVLRDSFVKSTHDLQSARGVALFDTCYDLSSKSSVEVPTVSFHFAN 425

Query: 427 GKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSSNK 486
           G ELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRV FDL+NSLVGFS NK
Sbjct: 426 GNELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLSNSLVGFSPNK 485

[Close]

BLAST of Carg01827 vs. ExPASy TrEMBL
Match: A0A0A0KUG1 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608450 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.8e-239
Identity = 424/481 (88.15%), Postives = 446/481 (92.72%), Query Frame = 0


Query: 7   LFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDESTVSDSSS 66
           LFLL LL++S S    RTL   P TSVLDV ASI  T+QVFA +P+S   DE+TVSD SS
Sbjct: 6   LFLLSLLFSSLSAFHCRTLHPTPTTSVLDVAASIQRTQQVFAVEPKSSTPDETTVSDPSS 65

Query: 67  LTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLEPLWN-- 126
           L+L LNSR+S+MK SH DYKSLTLSRL RDSARVRSLTAR+DLAIRGITG DLEPL N  
Sbjct: 66  LSLQLNSRISVMKASHSDYKSLTLSRLKRDSARVRSLTARIDLAIRGITGTDLEPLGNGG 125

Query: 127 GGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADC 186
           GGGSQFG EDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCA+C
Sbjct: 126 GGGSQFGTEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCAEC 185

Query: 187 YEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV 246
           YEQTDPIFEPTSSASFTSLSC+T+QCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV
Sbjct: 186 YEQTDPIFEPTSSASFTSLSCETEQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV 245

Query: 247 TLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTS 306
           TLGSTSL NIA+GCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS+STS
Sbjct: 246 TLGSTSLGNIAIGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSDSTS 305

Query: 307 TLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGIII 366
           TLDFNSPI PDAVTAPLHRNPNLDTFFYLG+TGMSVGG +LPIPETSFQMS+DGNGGII+
Sbjct: 306 TLDFNSPITPDAVTAPLHRNPNLDTFFYLGLTGMSVGGAVLPIPETSFQMSEDGNGGIIV 365

Query: 367 DSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHFAD 426
           DSGTAVTRLQTT YN+LRDAFVK THDLQ+ARGVALFDTCYDLSSKSRVEVPTVSFHFA+
Sbjct: 366 DSGTAVTRLQTTVYNVLRDAFVKSTHDLQTARGVALFDTCYDLSSKSRVEVPTVSFHFAN 425

Query: 427 GKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSSNK 486
           G ELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRV FDLANSLVGFS NK
Sbjct: 426 GNELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVGFDLANSLVGFSPNK 485

[Close]

BLAST of Carg01827 vs. ExPASy TrEMBL
Match: A0A6J1G1W1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449928 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.4e-236
Identity = 418/480 (87.08%), Postives = 443/480 (92.29%), Query Frame = 0


Query: 6   FLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDESTVSDSS 65
           +LFLL  ++AS SG LSR+LPH  +TSVLDV ASI  T ++FAF+ +SP+ +E +V DSS
Sbjct: 7   WLFLLIAVFASVSGGLSRSLPHRTKTSVLDVAASIQRTTEIFAFEVKSPVEEEKSVMDSS 66

Query: 66  SLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLEPLWNG 125
           SL L LNSR+SI+KTSH DYKSLTLSRL RDSARVRSLTAR+DL IRG TGADL    NG
Sbjct: 67  SLALQLNSRISIVKTSHSDYKSLTLSRLSRDSARVRSLTARIDLVIRGTTGADLRTFGNG 126

Query: 126 GGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCY 185
           GGS+F AEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCY
Sbjct: 127 GGSRFRAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADCY 186

Query: 186 EQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETVT 245
           EQTDPIFEPTSS SF SLSC+T+QCKSLDVSECRNGTCLYEVSYGDGS TVGDFVTETVT
Sbjct: 187 EQTDPIFEPTSSTSFASLSCETEQCKSLDVSECRNGTCLYEVSYGDGSSTVGDFVTETVT 246

Query: 246 LGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSESTST 305
           LGSTSL+NIALGCGHNNEGLF+GAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDS+S ST
Sbjct: 247 LGSTSLSNIALGCGHNNEGLFVGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSDSAST 306

Query: 306 LDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGIIID 365
           LDFNSPIPPDAV APLHRNPNLDTFFYLG+TG+SVGGEILPIPETSFQMSQDGNGGIIID
Sbjct: 307 LDFNSPIPPDAVIAPLHRNPNLDTFFYLGVTGLSVGGEILPIPETSFQMSQDGNGGIIID 366

Query: 366 SGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHFADG 425
           SGTAVTRLQTT YNLLRDAFVK T  LQSARGVALFDTCYDLSSKSRVEVPTVSFHFA+G
Sbjct: 367 SGTAVTRLQTTVYNLLRDAFVKSTPYLQSARGVALFDTCYDLSSKSRVEVPTVSFHFANG 426

Query: 426 KELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSSNKC 485
           KELPLPAKNYL+PVDSEGTFCFAFAPTDS LSILGNAQQQGTRVSFDL NSLVGFS NKC
Sbjct: 427 KELPLPAKNYLLPVDSEGTFCFAFAPTDSALSILGNAQQQGTRVSFDLTNSLVGFSPNKC 486

[Close]

BLAST of Carg01827 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 627.1 bits (1616), Expect = 1.2e-179
Identity = 317/483 (65.63%), Postives = 387/483 (80.12%), Query Frame = 0


Query: 5   FFLFLLPLLYASFSGVLSRTLPH--APRTSVLDVDASIHTTRQVFAFQPQSPMRDESTVS 64
           FF+F L     S S V SR LP      TS+L+V  SIH T+   +F+     ++E T S
Sbjct: 9   FFIFFL----TSHSSVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLN--QQEEQTHS 68

Query: 65  DSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLEPL 124
            SSS +L L+SRVS+  T H DYKSLTL+RL RD+ARV+SL  R+DLAI  I+ ADL+P+
Sbjct: 69  ASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPI 128

Query: 125 WNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCA 184
                ++   +D E+P++SG +QGSGEYF+RVGIG+P   VYMVLDTGSDV+W+QC PCA
Sbjct: 129 STMYTTE--EQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCA 188

Query: 185 DCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTE 244
           DCY QT+PIFEP+SS+S+  LSC T QC +L+VSECRN TCLYEVSYGDGSYTVGDF TE
Sbjct: 189 DCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATE 248

Query: 245 TVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLNASSFSYCLVDRDSES 304
           T+T+GST + N+A+GCGH+NEGLF+GAAGLLGLGGG L+ PSQLN +SFSYCLVDRDS+S
Sbjct: 249 TLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDS 308

Query: 305 TSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNGGI 364
            ST+DF + + PDAV APL RN  LDTF+YLG+TG+SVGGE+L IP++SF+M + G+GGI
Sbjct: 309 ASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGI 368

Query: 365 IIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSFHF 424
           IIDSGTAVTRLQT  YN LRD+FVK T DL+ A GVA+FDTCY+LS+K+ VEVPTV+FHF
Sbjct: 369 IIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHF 428

Query: 425 ADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGFSS 484
             GK L LPAKNY+IPVDS GTFC AFAPT S+L+I+GN QQQGTRV+FDLANSL+GFSS
Sbjct: 429 PGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSS 483

Query: 485 NKC 486
           NKC
Sbjct: 489 NKC 483

[Close]

BLAST of Carg01827 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 537.7 bits (1384), Expect = 9.4e-153
Identity = 273/501 (54.49%), Postives = 364/501 (72.65%), Query Frame = 0


Query: 1   MATAFFLFLLPL----LYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQP----- 60
           MA   FL LL +    L+ + +   SR+L   P+T+VLDV +S+  T+ + +  P     
Sbjct: 1   MAFPRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSL 60

Query: 61  ----QSPMRDESTVSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARM 120
                  + D    + SS L+L L+SR + + + H DYKSLTLSRL RDS+RV  + A++
Sbjct: 61  TTTKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKI 120

Query: 121 DLAIRGITGADLEPLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVL 180
             A+ G+  +DL+P++N   +++  ED  +P+VSGASQGSGEYFSR+G+G P   +Y+VL
Sbjct: 121 RFAVEGVDRSDLKPVYN-EDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVL 180

Query: 181 DTGSDVSWVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEV 240
           DTGSDV+W+QC PCADCY+Q+DP+F PTSS+++ SL+C   QC  L+ S CR+  CLY+V
Sbjct: 181 DTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQV 240

Query: 241 SYGDGSYTVGDFVTETVTLG-STSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQL 300
           SYGDGS+TVG+  T+TVT G S  + N+ALGCGH+NEGLF GAAGLLGLGGG LS  +Q+
Sbjct: 241 SYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM 300

Query: 301 NASSFSYCLVDRDSESTSTLDFNS-PIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEIL 360
            A+SFSYCLVDRDS  +S+LDFNS  +     TAPL RN  +DTF+Y+G++G SVGGE +
Sbjct: 301 KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKV 360

Query: 361 PIPETSFQMSQDGNGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDL-QSARGVALFDTC 420
            +P+  F +   G+GG+I+D GTAVTRLQT AYN LRDAF+K T +L + +  ++LFDTC
Sbjct: 361 VLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTC 420

Query: 421 YDLSSKSRVEVPTVSFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQ 480
           YD SS S V+VPTV+FHF  GK L LPAKNYLIPVD  GTFCFAFAPT S+LSI+GN QQ
Sbjct: 421 YDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQ 480

Query: 481 QGTRVSFDLANSLVGFSSNKC 486
           QGTR+++DL+ +++G S NKC
Sbjct: 481 QGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Carg01827 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.8e-112
Identity = 220/485 (45.36%), Postives = 298/485 (61.44%), Query Frame = 0


Query: 5   FFLFLLPLLYASFSGVLSRTLPHAPRTSVLDVDASIHTTRQVFAFQPQSPMRDESTVSDS 64
           FF FL   L+ S S  +S      P   ++DV     T          +   DES    S
Sbjct: 6   FFFFLHLHLHLSSSSSIS-----FPDFQIIDVLQPPLTVTATLPDFNNTHFSDES----S 65

Query: 65  SSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGITGADLEPLWN 124
           S  TL L  R      ++ ++     +R+ RD+ RV ++       +R I+G  +     
Sbjct: 66  SKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAI-------LRRISGKVIP---- 125

Query: 125 GGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVSWVQCAPCADC 184
              S++   DF S IVSG  QGSGEYF R+G+G PP   YMV+D+GSD+ WVQC PC  C
Sbjct: 126 SSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC 185

Query: 185 YEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSECRNGTCLYEVSYGDGSYTVGDFVTETV 244
           Y+Q+DP+F+P  S S+T +SC +  C  ++ S C +G C YEV YGDGSYT G    ET+
Sbjct: 186 YKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETL 245

Query: 245 TLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLN---ASSFSYCLVDRDSE 304
           T   T + N+A+GCGH N G+FIGAAGLLG+GGGS+SF  QL+     +F YCLV R ++
Sbjct: 246 TFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTD 305

Query: 305 STSTLDF-NSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGEILPIPETSFQMSQDGNG 364
           ST +L F    +P  A   PL RNP   +F+Y+G+ G+ VGG  +P+P+  F +++ G+G
Sbjct: 306 STGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDG 365

Query: 365 GIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFDTCYDLSSKSRVEVPTVSF 424
           G+++D+GTAVTRL T AY   RD F  +T +L  A GV++FDTCYDLS    V VPTVSF
Sbjct: 366 GVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSF 425

Query: 425 HFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNAQQQGTRVSFDLANSLVGF 484
           +F +G  L LPA+N+L+PVD  GT+CFAFA + + LSI+GN QQ+G +VSFD AN  VGF
Sbjct: 426 YFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGF 470

Query: 485 SSNKC 486
             N C
Sbjct: 486 GPNVC 470

[Close]

BLAST of Carg01827 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 402.5 bits (1033), Expect = 4.7e-112
Identity = 226/445 (50.79%), Postives = 288/445 (64.72%), Query Frame = 0


Query: 55  MRDESTVSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSLTARMDLAIRGI 114
           + DES    ++SL++ L+   ++   S      L   RL RDS RV+S+T+ +     G 
Sbjct: 49  LTDESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITS-LAAVSTGR 108

Query: 115 TGADLEPLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTGSDVS 174
                 P   GG        F   ++SG SQGSGEYF R+G+G P + VYMVLDTGSDV 
Sbjct: 109 NATKRTPRTAGG--------FSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVV 168

Query: 175 WVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSL-DVSEC---RNGTCLYEVSYG 234
           W+QC+PC  CY QTD IF+P  S +F ++ C ++ C+ L D SEC   R+ TCLY+VSYG
Sbjct: 169 WLQCSPCKACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYG 228

Query: 235 DGSYTVGDFVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLN--- 294
           DGS+T GDF TET+T     + ++ LGCGH+NEGLF+GAAGLLGLG G LSFPSQ     
Sbjct: 229 DGSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRY 288

Query: 295 ASSFSYCLVDRDSEST-----STLDF-NSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVG 354
              FSYCLVDR S  +     ST+ F N+ +P  +V  PL  NP LDTF+YL + G+SVG
Sbjct: 289 NGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVG 348

Query: 355 GEILP-IPETSFQMSQDGNGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVAL 414
           G  +P + E+ F++   GNGG+IIDSGT+VTRL   AY  LRDAF      L+ A   +L
Sbjct: 349 GSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSL 408

Query: 415 FDTCYDLSSKSRVEVPTVSFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILG 474
           FDTC+DLS  + V+VPTV FHF  G E+ LPA NYLIPV++EG FCFAFA T  +LSI+G
Sbjct: 409 FDTCFDLSGMTTVKVPTVVFHFGGG-EVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIG 468

Query: 475 NAQQQGTRVSFDLANSLVGFSSNKC 486
           N QQQG RV++DL  S VGF S  C
Sbjct: 469 NIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of Carg01827 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.3e-109
Identity = 224/443 (50.56%), Postives = 285/443 (64.33%), Query Frame = 0


Query: 52  QSPMRDESTVSDSSSLTLLLNSRVSIMKTSHIDYKSLTLSRLYRDSARVRSL-TARMDLA 111
           +S     S    SSS+TL L+   ++      D   L  SRL RDS RV+S+ T    + 
Sbjct: 57  ESEFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIP 116

Query: 112 IRGITGADLEPLWNGGGSQFGAEDFESPIVSGASQGSGEYFSRVGIGRPPSPVYMVLDTG 171
            R +T A        GG       F S +VSG SQGSGEYF+R+G+G P   VYMVLDTG
Sbjct: 117 GRNVTHAP-----RPGG-------FSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTG 176

Query: 172 SDVSWVQCAPCADCYEQTDPIFEPTSSASFTSLSCQTQQCKSLDVSEC--RNGTCLYEVS 231
           SD+ W+QCAPC  CY Q+DPIF+P  S ++ ++ C +  C+ LD + C  R  TCLY+VS
Sbjct: 177 SDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVS 236

Query: 232 YGDGSYTVGDFVTETVTLGSTSLTNIALGCGHNNEGLFIGAAGLLGLGGGSLSFPSQLN- 291
           YGDGS+TVGDF TET+T     +  +ALGCGH+NEGLF+GAAGLLGLG G LSFP Q   
Sbjct: 237 YGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGH 296

Query: 292 --ASSFSYCLVDRDSES--TSTLDFNSPIPPDAVTAPLHRNPNLDTFFYLGMTGMSVGGE 351
                FSYCLVDR + S  +S +  N+ +   A   PL  NP LDTF+Y+G+ G+SVGG 
Sbjct: 297 RFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGT 356

Query: 352 ILP-IPETSFQMSQDGNGGIIIDSGTAVTRLQTTAYNLLRDAFVKKTHDLQSARGVALFD 411
            +P +  + F++ Q GNGG+IIDSGT+VTRL   AY  +RDAF      L+ A   +LFD
Sbjct: 357 RVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFD 416

Query: 412 TCYDLSSKSRVEVPTVSFHFADGKELPLPAKNYLIPVDSEGTFCFAFAPTDSTLSILGNA 471
           TC+DLS+ + V+VPTV  HF  G ++ LPA NYLIPVD+ G FCFAFA T   LSI+GN 
Sbjct: 417 TCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNI 476

Query: 472 QQQGTRVSFDLANSLVGFSSNKC 486
           QQQG RV +DLA+S VGF+   C
Sbjct: 477 QQQGFRVVYDLASSRVGFAPGGC 484

The following BLAST results are available for this feature:

BLAST of Carg01827 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6601306.1	6.3e-276	100.00	Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022957517.1	2.0e-274	99.38	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata]	[more]
XP_023544196.1	1.3e-268	98.14	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022983595.1	3.5e-266	97.73	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima]	[more]
XP_038891764.1	1.2e-242	89.56	protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida]	[more]

BLAST of Carg01827 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LS40	1.3e-151	54.49	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LHE3	3.9e-111	45.36	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	1.8e-108	50.56	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	4.4e-70	40.56	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	2.4e-68	41.29	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of Carg01827 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GZC0	9.9e-275	99.38	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1J6A9	1.7e-266	97.73	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A1S3BFC3	4.9e-242	88.57	protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC10348926...	[more]
A0A0A0KUG1	1.8e-239	88.15	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G60845...	[more]
A0A6J1G1W1	1.4e-236	87.08	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of Carg01827 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G25510.1	1.2e-179	65.63	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	9.4e-153	54.49	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	2.8e-112	45.36	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	4.7e-112	50.79	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	1.3e-109	50.56	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 312..485 e-value: 4.8E-51 score: 175.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 128..308 e-value: 8.9E-51 score: 174.6
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 144..485
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 331..481 e-value: 5.6E-36 score: 123.8
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 150..307 e-value: 7.7E-49 score: 166.3
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 12..485
None	No IPR available	PANTHER	PTHR13683:SF775	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 12..485
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 165..176
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 150..481 score: 44.833229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01827-RA	Carg01827-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity