Carg01448 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01448
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	15.7 kDa heat shock protein, peroxisomal
Location	Carg_Chr15: 3932198 .. 3934068 (-)
RNA-Seq Expression	Carg01448
Synteny	Carg01448

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGATCGTTTCAGTTTCCAGAGATTTTTAGTTTCGATCAGTTCCGTATATAAATACAATTTTGCCGCAGCTGCCGTCTGTGGAACTTCAATTCCTTAGGCTTGCTTTCAATGGCGAATGATCTCTTTGGATATCCTCTTCGGCGCTTCTTCTGGAGCCCGGCCGTGTTCCGGGGACCGTCCGGCACCATTGCGCTCATGGATTGGCTCGAAACATCCAATGCTCACATCTTCAAGGTTGACGTTCCAGGTAACTTTTTATTATTCTTTTTAATTTTCATTTCCGCTTGGCTTTGAATGCTGATTTACGCAATACGGCCTTGATTTGAGGTGTGTGAAAGTTATGCCTCTGATGTCTATGCGGAATGGACGTGCTCTAGGTTTTGTTGATTTTTGTTCGTGTTGTTTTTTCTTTTGTTTGAGTTCTTTTCCTTTTCTTGGATTATTGACTTGTTTGGTTCATTCGGAGTGATTATAAACAACTTATTTACTCGGTTCAGTTGGAGGAATTGTTTTATTTTTTGGAGTTTAGATTCAATGTGCAATCAATCTTTTAGTTCAATGAGTTTGAACAGTGCTTCCTGTCTTGGATGATTGAATCGTCGTCGCCTATGGAACTTGTTGAAAATCAATGAAACTTAAACACTGTTCGAGTTTAGATAAACATAGTACTTAATCGAGTGTTTATATATTTGGTTTCTTAAGGTGGATTAAATGCTAGTACATAGAAGAGAAAATCTCTGCAACGCTGGTACATATTTCTCCTGTTCAGCATTCCACACCTAATAAGATGAAATGTTGTGGCAAGTAACGGATGAATGGATGAATGAGCGTAGTACTTGAGAGTTTTCCTTGTACGTCTGAAGCTAGTATGTAGATCCTAGAGGGAAGAACAAATCGAAAAATGAAAATCTGGAGATGGCGGCCAACTAAAATTATTACTCCTAACAAAATAAGTGAAAAGGAAAATCACCAATTGTTCATACGGAACGAGTTCACAAGCTTTTGTAGGAGATTGTGCAAGGTTAACTCGAACAGTTACAGACATCTCTTCACAAAATACATGTTTAGTTCATCACTCTCTTAAATTCTCTCTGTTTTTCTTCTCCATGCGTTTCCTAATTCGAAATCTGCACAGGGTTCAGCAAGGACGAGATAACAGTACAGGTTGACGAGGCGAATGTCCTACACATTCAAGGAACGAGTGGTAAAGAGGAAGCTCATGGAAAGGAGGCAATCTGGCATTTGGCGGAAAGACAAACAGGGAAGAGGGGATTTTCCAGGGAGATTGAACTGCCAGAAAACGTTAAGTTGGATAAAATCAAGGCGCAGGTTGAGGATGGGCTCCTCACCATTGTTGTCCCAAGAGATACAGCCCCAAGGCCATCAAAGGTCAGGAATATCAACATTACGAGCAAGCTTTGAGATCTGAAACTTGCAGGAATTCAACGATTAGCTGAATAATGCATAAGTTTTTGGAGTGGGAGTGCCGTTGAAGGTGTGTTGTATCTGTCTGTTCATAAATAATGGGACGTTTTTTCCTTTTGTGTTTGAGGTACCACTTTTTCAACAAATATATGATATAAAATATTCCTGATAATTGTTCTTTTAGAATCGAAGATAATTGTTTTGAACATTATTTTTTATTTTTATAAATATTTTTTTAAAAATTTATTATATTACTTCGTTACTATTTTTATTAAAACAAATTATTAAAATATGAGAATCGATCATTTCTATATCGCTACTCATCTCGAACCAATGAACCTTATTACTGTCACAAACTATTAACGCTAATGACTACTATAGATGATCAATATTGCCTAAGTATAAAATTAATTTGAGTTTATAATAATATTTTAAGTATAAAATT

mRNA sequence

GAGATCGTTTCAGTTTCCAGAGATTTTTAGTTTCGATCAGTTCCGTATATAAATACAATTTTGCCGCAGCTGCCGTCTGTGGAACTTCAATTCCTTAGGCTTGCTTTCAATGGCGAATGATCTCTTTGGATATCCTCTTCGGCGCTTCTTCTGGAGCCCGGCCGTGTTCCGGGGACCGTCCGGCACCATTGCGCTCATGGATTGGCTCGAAACATCCAATGCTCACATCTTCAAGGTTGACGTTCCAGGGTTCAGCAAGGACGAGATAACAGTACAGGTTGACGAGGCGAATGTCCTACACATTCAAGGAACGAGTGGTAAAGAGGAAGCTCATGGAAAGGAGGCAATCTGGCATTTGGCGGAAAGACAAACAGGGAAGAGGGGATTTTCCAGGGAGATTGAACTGCCAGAAAACGTTAAGTTGGATAAAATCAAGGCGCAGGTTGAGGATGGGCTCCTCACCATTGTTGTCCCAAGAGATACAGCCCCAAGGCCATCAAAGGTCAGGAATATCAACATTACGAGCAAGCTTTGAGATCTGAAACTTGCAGGAATTCAACGATTAGCTGAATAATGCATAAGTTTTTGGAGTGGGAGTGCCGTTGAAGGTGTGTTGTATCTGTCTGTTCATAAATAATGGGACGTTTTTTCCTTTTGTGTTTGAGGTACCACTTTTTCAACAAATATATGATATAAAATATTCCTGATAATTGTTCTTTTAGAATCGAAGATAATTGTTTTGAACATTATTTTTTATTTTTATAAATATTTTTTTAAAAATTTATTATATTACTTCGTTACTATTTTTATTAAAACAAATTATTAAAATATGAGAATCGATCATTTCTATATCGCTACTCATCTCGAACCAATGAACCTTATTACTGTCACAAACTATTAACGCTAATGACTACTATAGATGATCAATATTGCCTAAGTATAAAATTAATTTGAGTTTATAATAATATTTTAAGTATAAAATT

Coding sequence (CDS)

ATGGCGAATGATCTCTTTGGATATCCTCTTCGGCGCTTCTTCTGGAGCCCGGCCGTGTTCCGGGGACCGTCCGGCACCATTGCGCTCATGGATTGGCTCGAAACATCCAATGCTCACATCTTCAAGGTTGACGTTCCAGGGTTCAGCAAGGACGAGATAACAGTACAGGTTGACGAGGCGAATGTCCTACACATTCAAGGAACGAGTGGTAAAGAGGAAGCTCATGGAAAGGAGGCAATCTGGCATTTGGCGGAAAGACAAACAGGGAAGAGGGGATTTTCCAGGGAGATTGAACTGCCAGAAAACGTTAAGTTGGATAAAATCAAGGCGCAGGTTGAGGATGGGCTCCTCACCATTGTTGTCCCAAGAGATACAGCCCCAAGGCCATCAAAGGTCAGGAATATCAACATTACGAGCAAGCTTTGA

Protein sequence

MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEANVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIVVPRDTAPRPSKVRNINITSKL

Homology

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BLAST of Carg01448 vs. NCBI nr
Match: KAG6579046.1 (15.7 kDa heat shock protein, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016570.1 15.7 kDa heat shock protein, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 288.1 bits (736), Expect = 4.1e-74
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA
Sbjct: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VPRDTAPRPSKVRNINITSKL
Sbjct: 121 VPRDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Carg01448 vs. NCBI nr
Match: XP_022939289.1 (15.7 kDa heat shock protein, peroxisomal [Cucurbita moschata])

HSP 1 Score: 285.0 bits (728), Expect = 3.4e-73
Identity = 139/141 (98.58%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           MANDLFGYPLRRFFWSP VFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA
Sbjct: 1   MANDLFGYPLRRFFWSPPVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VP+DTAPRPSKVRNINITSKL
Sbjct: 121 VPKDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Carg01448 vs. NCBI nr
Match: XP_023550919.1 (15.7 kDa heat shock protein, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 284.6 bits (727), Expect = 4.5e-73
Identity = 139/141 (98.58%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           MANDLFGYPLRRFFWSPAV RGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA
Sbjct: 1   MANDLFGYPLRRFFWSPAVLRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VP+DTAPRPSKVRNINITSKL
Sbjct: 121 VPKDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Carg01448 vs. NCBI nr
Match: XP_022993441.1 (15.7 kDa heat shock protein, peroxisomal [Cucurbita maxima])

HSP 1 Score: 283.1 bits (723), Expect = 1.3e-72
Identity = 137/141 (97.16%), Postives = 141/141 (100.00%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQV+EA
Sbjct: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVNEA 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           NVLHIQGTSGKEEAHGK+AIWHLAERQTGKRGFSRE+ELPENVKLDKIKAQVEDGLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKDAIWHLAERQTGKRGFSRELELPENVKLDKIKAQVEDGLLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VP+DTAPRPSKVRNINITSKL
Sbjct: 121 VPKDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Carg01448 vs. NCBI nr
Match: ADU55788.1 (HSP15.9 [Citrullus lanatus])

HSP 1 Score: 249.6 bits (636), Expect = 1.6e-62
Identity = 119/141 (84.40%), Postives = 131/141 (92.91%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           MANDLFGYPLRRF WSPAVFR PSGT+AL+DWLETSNAHIFKVDVPGFSKDE+ V+V+E 
Sbjct: 1   MANDLFGYPLRRFLWSPAVFRQPSGTVALLDWLETSNAHIFKVDVPGFSKDELKVRVEEG 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           NV+HI+G SGKEE+ GKEAIWHL ERQ GKR FSREIELPENVKLD+IKAQ+E+GLLTIV
Sbjct: 61  NVMHIEGMSGKEESVGKEAIWHLGERQIGKRSFSREIELPENVKLDQIKAQLENGLLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VP+DTAPRPSKVRNINI SKL
Sbjct: 121 VPKDTAPRPSKVRNINIISKL 141

[Close]

BLAST of Carg01448 vs. ExPASy Swiss-Prot
Match: Q9FHQ3 (15.7 kDa heat shock protein, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=HSP15.7 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.2e-33
Identity = 74/141 (52.48%), Postives = 104/141 (73.76%), Query Frame = 0


Query: 5   LFGYPLRRF-FWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEANVL 64
           +F YP RRF  WS +         AL+DW+E++N+HIFK++VPG++K++I VQ++E NVL
Sbjct: 6   IFLYPFRRFQEWSRST--------ALIDWMESNNSHIFKINVPGYNKEDIKVQIEEGNVL 65

Query: 65  HIQGTSGKEEAHGKEAIWHLAERQT---GKRGFSREIELPENVKLDKIKAQVEDGLLTIV 124
            I+G   KEE   +  +WH+AER+    G   F R IELPENVK+D++KA VE+G+LT+V
Sbjct: 66  SIRGEGIKEEKK-ENLVWHVAEREAFSGGGSEFLRRIELPENVKVDQVKAYVENGVLTVV 125

Query: 125 VPRDTAPRPSKVRNINITSKL 142
           VP+DT+ + SKVRN+NITSKL
Sbjct: 126 VPKDTSSKSSKVRNVNITSKL 137

[Close]

BLAST of Carg01448 vs. ExPASy Swiss-Prot
Match: Q652V8 (16.0 kDa heat shock protein, peroxisomal OS=Oryza sativa subsp. japonica OX=39947 GN=HSP16.0 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 9.6e-26
Identity = 60/149 (40.27%), Postives = 96/149 (64.43%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWS---PAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQV 60
           MA+  FG P RR  +    P  +   S T A MDW+ET  +H+ +++VPG  KD++ VQV
Sbjct: 1   MADLFFGGPFRRILYGRPFPPDWASASAT-AAMDWVETPTSHVLRINVPGLGKDDVKVQV 60

Query: 61  DEANVLHIQG-----TSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQV 120
           ++ NVL ++G      + KE    K+ +WH+AER  G+  F+RE+ LP  V++++I+A V
Sbjct: 61  EDGNVLTVRGAAPHAAAEKEREREKDVVWHVAER--GRPEFAREVALPAEVRVEQIRASV 120

Query: 121 EDGLLTIVVPRDTAPRPSKVRNINITSKL 142
           ++G+LT+VVP++ AP   + R I ++SKL
Sbjct: 121 DNGVLTVVVPKEPAPARPRTRPIAVSSKL 146

[Close]

BLAST of Carg01448 vs. ExPASy Swiss-Prot
Match: Q05832 (18.3 kDa class I heat shock protein OS=Oxybasis rubra OX=3560 GN=HSP18 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 4.9e-22
Identity = 50/110 (45.45%), Postives = 76/110 (69.09%), Query Frame = 0


Query: 28  ALMDWLETSNAHIFKVDVPGFSKDEITVQVDEANVLHIQGTSGKEEAHGKEAIWHLAERQ 87
           A +DW ET  AH+FK D+PG  K+E+ V+V++ NVL I G   +E+   K   WH  ER 
Sbjct: 55  ARIDWKETPEAHVFKADLPGVKKEEVKVEVEDGNVLRISGQRAREKEE-KNDTWHRVERS 114

Query: 88  TGKRGFSREIELPENVKLDKIKAQVEDGLLTIVVPRDTAPRPSKVRNINI 138
           +G+  F R+  LPEN K+D++KA +E+G+LT+ VP++ AP+P +V+ IN+
Sbjct: 115 SGQ--FMRKFRLPENAKVDQVKAGMENGVLTVTVPKNEAPKP-QVKAINV 160

[Close]

BLAST of Carg01448 vs. ExPASy Swiss-Prot
Match: Q9XIE3 (17.6 kDa class I heat shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6A PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 3.2e-21
Identity = 49/112 (43.75%), Postives = 80/112 (71.43%), Query Frame = 0


Query: 28  ALMDWLETSNAHIFKVDVPGFSKDEITVQVDEANVLHIQGTSGKEEAHGKEAIWHLAERQ 87
           A +DW ET+ AH+FK D+PG  K+E+ V++++ +VL I G    E+   K+  WH  ER 
Sbjct: 46  ARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEE-KQDTWHRVERS 105

Query: 88  TGKRGFSREIELPENVKLDKIKAQVEDGLLTIVVPR-DTAPRPSKVRNINIT 139
           +G  GFSR+  LPENVK+D++KA +E+G+LT+ VP+ +T  + ++V++I+I+
Sbjct: 106 SG--GFSRKFRLPENVKMDQVKASMENGVLTVTVPKVETNKKKAQVKSIDIS 154

[Close]

BLAST of Carg01448 vs. ExPASy Swiss-Prot
Match: Q84Q77 (17.9 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP17.9A PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.2e-21
Identity = 51/111 (45.95%), Postives = 73/111 (65.77%), Query Frame = 0


Query: 28  ALMDWLETSNAHIFKVDVPGFSKDEITVQVDEANVLHIQGTSGKEEAHGKEAIWHLAERQ 87
           A +DW ET  AH+FK DVPG  K+E+ V+VD+ N+L I G   KE+   K   WH  ER 
Sbjct: 54  ARIDWKETPEAHVFKADVPGLKKEEVKVEVDDGNILQISGERNKEQEE-KTDQWHRVERS 113

Query: 88  TGKRGFSREIELPENVKLDKIKAQVEDGLLTIVVPRDTAPRPSKVRNINIT 139
           +GK  F R   LP+N K ++IKA +E+G+LT+ VP++ A +P  V++I I+
Sbjct: 114 SGK--FLRRFRLPDNAKPEQIKASMENGVLTVTVPKEEAKKPD-VKSIQIS 160

[Close]

BLAST of Carg01448 vs. ExPASy TrEMBL
Match: A0A6J1FGQ5 (15.7 kDa heat shock protein, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445251 PE=3 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.7e-73
Identity = 139/141 (98.58%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           MANDLFGYPLRRFFWSP VFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA
Sbjct: 1   MANDLFGYPLRRFFWSPPVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VP+DTAPRPSKVRNINITSKL
Sbjct: 121 VPKDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Carg01448 vs. ExPASy TrEMBL
Match: A0A6J1JWB5 (15.7 kDa heat shock protein, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489455 PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 6.3e-73
Identity = 137/141 (97.16%), Postives = 141/141 (100.00%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQV+EA
Sbjct: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVNEA 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           NVLHIQGTSGKEEAHGK+AIWHLAERQTGKRGFSRE+ELPENVKLDKIKAQVEDGLLTIV
Sbjct: 61  NVLHIQGTSGKEEAHGKDAIWHLAERQTGKRGFSRELELPENVKLDKIKAQVEDGLLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VP+DTAPRPSKVRNINITSKL
Sbjct: 121 VPKDTAPRPSKVRNINITSKL 141

[Close]

BLAST of Carg01448 vs. ExPASy TrEMBL
Match: H6TB41 (HSP15.9 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.8e-63
Identity = 119/141 (84.40%), Postives = 131/141 (92.91%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           MANDLFGYPLRRF WSPAVFR PSGT+AL+DWLETSNAHIFKVDVPGFSKDE+ V+V+E 
Sbjct: 1   MANDLFGYPLRRFLWSPAVFRQPSGTVALLDWLETSNAHIFKVDVPGFSKDELKVRVEEG 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           NV+HI+G SGKEE+ GKEAIWHL ERQ GKR FSREIELPENVKLD+IKAQ+E+GLLTIV
Sbjct: 61  NVMHIEGMSGKEESVGKEAIWHLGERQIGKRSFSREIELPENVKLDQIKAQLENGLLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VP+DTAPRPSKVRNINI SKL
Sbjct: 121 VPKDTAPRPSKVRNINIISKL 141

[Close]

BLAST of Carg01448 vs. ExPASy TrEMBL
Match: A0A6J1CHX8 (15.7 kDa heat shock protein, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111011798 PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 6.4e-57
Identity = 111/141 (78.72%), Postives = 124/141 (87.94%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           MA+D FGYPLRRFFWSPAVFR P G +AL+DWLETSNAH FKV+VPGFSKDEI VQVDE 
Sbjct: 1   MADDFFGYPLRRFFWSPAVFREPFGAVALLDWLETSNAHTFKVNVPGFSKDEIKVQVDEG 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           N+LHI+G+ GKEE   K+ IWHLAERQ  KR FSREIELPENVK+D+IKAQVE+G+LTIV
Sbjct: 61  NILHIKGSRGKEEPVAKDTIWHLAERQREKREFSREIELPENVKVDQIKAQVENGVLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VP+DTA R SKVRNINITSKL
Sbjct: 121 VPKDTATRSSKVRNINITSKL 141

[Close]

BLAST of Carg01448 vs. ExPASy TrEMBL
Match: A0A5A7TS74 (15.7 kDa heat shock protein, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00210 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 4.1e-56
Identity = 107/141 (75.89%), Postives = 123/141 (87.23%), Query Frame = 0


Query: 1   MANDLFGYPLRRFFWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEA 60
           M NDLFGYPLRRF W+PA FR PSG +AL+DWLETS+AHIFKVDVPGFSKDE+ V+++E 
Sbjct: 1   MTNDLFGYPLRRFPWTPASFRQPSGAVALLDWLETSSAHIFKVDVPGFSKDELKVEIEEG 60

Query: 61  NVLHIQGTSGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIV 120
           NV+HI G SG+EE+  KE IWHL ERQTGKR FSREIELPENVKLD+IKAQ+E+GLLTIV
Sbjct: 61  NVMHITGNSGEEESVEKEVIWHLGERQTGKRSFSREIELPENVKLDQIKAQLENGLLTIV 120

Query: 121 VPRDTAPRPSKVRNINITSKL 142
           VP+DT PRPSKV+ I I SKL
Sbjct: 121 VPKDTTPRPSKVKKIKIISKL 141

[Close]

BLAST of Carg01448 vs. TAIR 10
Match: AT5G37670.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 8.8e-35
Identity = 74/141 (52.48%), Postives = 104/141 (73.76%), Query Frame = 0


Query: 5   LFGYPLRRF-FWSPAVFRGPSGTIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEANVL 64
           +F YP RRF  WS +         AL+DW+E++N+HIFK++VPG++K++I VQ++E NVL
Sbjct: 6   IFLYPFRRFQEWSRST--------ALIDWMESNNSHIFKINVPGYNKEDIKVQIEEGNVL 65

Query: 65  HIQGTSGKEEAHGKEAIWHLAERQT---GKRGFSREIELPENVKLDKIKAQVEDGLLTIV 124
            I+G   KEE   +  +WH+AER+    G   F R IELPENVK+D++KA VE+G+LT+V
Sbjct: 66  SIRGEGIKEEKK-ENLVWHVAEREAFSGGGSEFLRRIELPENVKVDQVKAYVENGVLTVV 125

Query: 125 VPRDTAPRPSKVRNINITSKL 142
           VP+DT+ + SKVRN+NITSKL
Sbjct: 126 VPKDTSSKSSKVRNVNITSKL 137

[Close]

BLAST of Carg01448 vs. TAIR 10
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 2.3e-22
Identity = 49/112 (43.75%), Postives = 80/112 (71.43%), Query Frame = 0


Query: 28  ALMDWLETSNAHIFKVDVPGFSKDEITVQVDEANVLHIQGTSGKEEAHGKEAIWHLAERQ 87
           A +DW ET+ AH+FK D+PG  K+E+ V++++ +VL I G    E+   K+  WH  ER 
Sbjct: 46  ARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEE-KQDTWHRVERS 105

Query: 88  TGKRGFSREIELPENVKLDKIKAQVEDGLLTIVVPR-DTAPRPSKVRNINIT 139
           +G  GFSR+  LPENVK+D++KA +E+G+LT+ VP+ +T  + ++V++I+I+
Sbjct: 106 SG--GFSRKFRLPENVKMDQVKASMENGVLTVTVPKVETNKKKAQVKSIDIS 154

[Close]

BLAST of Carg01448 vs. TAIR 10
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 3.9e-22
Identity = 49/114 (42.98%), Postives = 82/114 (71.93%), Query Frame = 0


Query: 26  TIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEANVLHIQGTSGKEEAHGKEAIWHLAE 85
           T A +DW ET+ AH+FK D+PG  K+E+ V++++ +VL I G    E+   K+  WH  E
Sbjct: 46  TNARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEE-KQDTWHRVE 105

Query: 86  RQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIVVPR-DTAPRPSKVRNINIT 139
           R +G+  FSR+ +LPENVK+D++KA +E+G+LT+ VP+ + A + ++V++I+I+
Sbjct: 106 RSSGQ--FSRKFKLPENVKMDQVKASMENGVLTVTVPKVEEAKKKAQVKSIDIS 156

[Close]

BLAST of Carg01448 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 101.7 bits (252), Expect = 5.0e-22
Identity = 51/113 (45.13%), Postives = 75/113 (66.37%), Query Frame = 0


Query: 26  TIALMDWLETSNAHIFKVDVPGFSKDEITVQVDEANVLHIQGTSGKEEAHGKEAIWHLAE 85
           T A +DW ET  AH+FK D+PG  K+E+ V+V++ NVL I G   KE    K   WH  E
Sbjct: 50  TNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQISGERSKENEE-KNDKWHRVE 109

Query: 86  RQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIVVPRDTAPRPSKVRNINIT 139
           R +GK  F R   LPEN K++++KA +E+G+LT+VVP+    +P +V++I+I+
Sbjct: 110 RASGK--FMRRFRLPENAKMEEVKATMENGVLTVVVPKAPEKKP-QVKSIDIS 158

[Close]

BLAST of Carg01448 vs. TAIR 10
Match: AT3G46230.1 (heat shock protein 17.4 )

HSP 1 Score: 99.0 bits (245), Expect = 3.3e-21
Identity = 49/130 (37.69%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 14  FWSPAVFRGPSGTIAL-----MDWLETSNAHIFKVDVPGFSKDEITVQVDEANVLHIQGT 73
           F +P +   P+  +A      +DW ET  AH+FK DVPG  K+E+ V+V++ N+L I G 
Sbjct: 30  FLTPGLTNAPAKDVAAFTNAKVDWRETPEAHVFKADVPGLKKEEVKVEVEDGNILQISGE 89

Query: 74  SGKEEAHGKEAIWHLAERQTGKRGFSREIELPENVKLDKIKAQVEDGLLTIVVPRDTAPR 133
              E    K   WH  ER +GK  F R   LPEN K++++KA +E+G+L++ VP+    +
Sbjct: 90  RSSENEE-KSDTWHRVERSSGK--FMRRFRLPENAKVEEVKASMENGVLSVTVPKVQESK 149

Query: 134 PSKVRNINIT 139
           P +V++++I+
Sbjct: 150 P-EVKSVDIS 155

The following BLAST results are available for this feature:

BLAST of Carg01448 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6579046.1	4.1e-74	100.00	15.7 kDa heat shock protein, peroxisomal, partial [Cucurbita argyrosperma subsp....	[more]
XP_022939289.1	3.4e-73	98.58	15.7 kDa heat shock protein, peroxisomal [Cucurbita moschata]	[more]
XP_023550919.1	4.5e-73	98.58	15.7 kDa heat shock protein, peroxisomal [Cucurbita pepo subsp. pepo]	[more]
XP_022993441.1	1.3e-72	97.16	15.7 kDa heat shock protein, peroxisomal [Cucurbita maxima]	[more]
ADU55788.1	1.6e-62	84.40	HSP15.9 [Citrullus lanatus]	[more]

BLAST of Carg01448 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHQ3	1.2e-33	52.48	15.7 kDa heat shock protein, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=HSP1...	[more]
Q652V8	9.6e-26	40.27	16.0 kDa heat shock protein, peroxisomal OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q05832	4.9e-22	45.45	18.3 kDa class I heat shock protein OS=Oxybasis rubra OX=3560 GN=HSP18 PE=2 SV=1	[more]
Q9XIE3	3.2e-21	43.75	17.6 kDa class I heat shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6...	[more]
Q84Q77	4.2e-21	45.95	17.9 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]

BLAST of Carg01448 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FGQ5	1.7e-73	98.58	15.7 kDa heat shock protein, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1JWB5	6.3e-73	97.16	15.7 kDa heat shock protein, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
H6TB41	7.8e-63	84.40	HSP15.9 OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]
A0A6J1CHX8	6.4e-57	78.72	15.7 kDa heat shock protein, peroxisomal OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A5A7TS74	4.1e-56	75.89	15.7 kDa heat shock protein, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of Carg01448 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37670.1	8.8e-35	52.48	HSP20-like chaperones superfamily protein	[more]
AT1G59860.1	2.3e-22	43.75	HSP20-like chaperones superfamily protein	[more]
AT1G07400.1	3.9e-22	42.98	HSP20-like chaperones superfamily protein	[more]
AT5G59720.1	5.0e-22	45.13	heat shock protein 18.2	[more]
AT3G46230.1	3.3e-21	37.69	heat shock protein 17.4	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 31..137 e-value: 7.7E-23 score: 80.6
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 21..138 score: 24.284586
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 4..138 e-value: 7.2E-31 score: 108.9
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 9..132
None	No IPR available	PANTHER	PTHR11527:SF142	15.7 KDA HEAT SHOCK PROTEIN, PEROXISOMAL	coord: 5..140
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 5..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01448-RA	Carg01448-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding