Carg01422 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01422
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerase II subunit RPB7
Location	Carg_Chr15: 4086028 .. 4089834 (+)
RNA-Seq Expression	Carg01422
Synteny	Carg01422

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CACTTTGTCCCGCCGCCGCCGCCTCCCTACCAGTACGTCTTTCTTCACAGCAACTCAAATTAGCATTTGTGTAACGAAAGAAACAGGGAGTTGTTTGCTTAACAGAACAATCTCAGCTTATCAATGACTGTAGATTATAATCGGGTTTTAATGTTCATTGGTTCTGGTTCACTGTCTCATACCCATATCATCGATTCAGCATTTTCTTCTCTCTCTTTTTTCTTGCTCTATCACAAATGGAGTCCACAAAACCCTCTTCTGCAATTGTGGAAGAACATAGATTCTTGCTTCTTCCACTTGTTCTTGTTCTTGTTCTTCTTTATCGCACTCTTAATTTCTTTAAATGGGGTTTCCTTCAGGTACAGGGAGCCACTATGTTCTTCCACATAGTTTTGGAGAGGAACATGCAGCTGCATCCACGGCACTTTGGCCGTAATCTCCGTGAAAATCTGGTGTCCAAACTCATGAAAGATGTTGAGGGTACTTGCAGGTTTGTTAACTTCACCCCCACCGCTTGTATCCTGCTACAACATTGCTGTTGATCTTGTTTTCTTTTTGTTAAATTTACCTTTTCGATAAGACCCTGAATGAGATCTCATGAAACCTTCTTTATAAGGGTGTGGAAACCCCTCCCTAGCAAACGCATTTTGAAAACCTTAGGGGAAGTCCTAAGAGGACAATATCTGCTAGCAGTTGGGTCGGGTCGGGTCGTTTCACTTTGCTTCACAACTAAGTATCCTAAAAGCATTCATGATTTGAACTAGTTTAGCTGGGCAAGATGGTTGTGTAGTTTCTTGTAGGAAAACCTCCCTGGTAAGTCTTAATTTGTGAGATCCCACGTCGGTTGGAGGGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTTCCTAGCAGACGCGTTTTAAAAACCTTGGGGGGAAACCCGTAAGGGAAAGCTCAAATAAGACAATATCTGCTGGCGGTGGGCTTGGGTTGTTACAAATGATGTCAGAGCCAGACACCGGATGGTGTGCTAGTGAGGACATTGGGACCCTAAGGGGGTGGATTGTGAGATCCCACATTGGTTGGAGAGAGGAATGGAGTATCCCTTACAAGGGTGTGGAGGTTACTTCTACCTAGCATACGCGTTTTAAAACCTTGAGGGAAAGCCTAGAAGGAAAGCCCAAAGAGGACAATATTTGCTAGCGGTGGAGTTGGATTGTTACAAATGGTATAAGAGCCCGACACTGGGCGGTATGCCAGCGAGGTTGCCGGGTCCTGAAGGGGGGTAGATTGTGAGATTTCACATCAATTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAACAGACGTGTTTTAAAAACCTTGAGGGGAAGCTCGGAAGAGAAACCTCAAAAAAGACAATATCTACTAGCGGTGGGTTTGGGCTGTTGCAAATGGTATCAGAGCCAGACACTAGACAATGTGCCAGCGAGGACGCTAGGCCCCTAAGGGGGTGGATTGTGAGATCCCACATCGGTTGGAAAGGGGAACGAAACATCCCTTGTAAGGGGTGTAGAAACCTCTCCGTAGCAGATGCGTTTTAAAACCTCGAGGGAAAGCCTAGAAGGAAAAGCCCAAAGAGGACAATATCTGCTAGTTGTGGGCTTGGACTGTTACAACATGTCAGTTCATGAGCGGACAAAAGCCAATAAAAATGGTAAAACTATTTGTTGGATGATATTATTCTTTTAAAAGGTTTGATACTTCTTTCAACTAGTGAACAATTAGATGGATTATGTTATCGAAAAGTTTTGTACTTCGACAGTGGTCGACATGGTTTCGTAGTAGCAATTACGGGAATTGAGAACATCGGTAAAGGATTGATTAGCGATGGGACAGGTTTCGTCACTTTTCCCGTTAAATACCAATGCGTCGTGTTTCGACCATTCAAGGGAGAAATCTTAGAAGCAATTGTTACTATGGTAAACAAGGTATCTATCTGGCACCATGGCACCCTTTTTTGATATCTAGCATGTGATTAATGAAGTTCTTTTGAATAGTAAATATGAATATGACTGCCATTGCAACTATTGTAGATGGGTTTCTTTGCTGAAGCTGGACCTGTTCAGATTTTTGTTTCAAACCATGTAAGTATATGTTTATAATCCATTTTCATATATATGCGAGCGGTGGGTTTTGGCGGTTACAAATGGTATCAGAGCCAGACACTGGATGATGTTGTGCCAACGAGGAGGCTGAGCCCCGAAGGGGGGTGGACAAGAGGCGGTGTGCCAACAAGGACGTTGGGCCTTGAAGAGGGTAGATTGGGGGGTCCCACATCCATTAGAGAAGGGAACAAGTGTCCGCGAGGATGCTGGGCCCCGAAGGGGGTGGATTGTGAGATCTTACATTGGTTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGTAGAAACCTCTTCCTAGTAGAGGCGTTTAGAAACCTTGAGGGGTAGCTCGAAAGGGGAAACCCAAAGAGGACAATATATGCTAGCGGTGGGCTTTGGCGGTTACAAATGGTATCAAAGCCAGACATCGGAAGATGTTGTGCTAGCGAGGAGGTTGAAACTCGAAAGGGGGTGGACATGAGGCGGTGTGCCAAAAAGGACGCTGGGCTCTGAAGATGGTAGATTGGGAGGTCCCACATCTATTAGAGAAGGGAACAAGTGCCAGCGAGGATGCTGGGCTCCGAAGGGGGTGGATTGTGAGATCCCACATCGATTGGGGAGAAAGGGGGTGGATTGTGAGATCTTACATCGATTGGGGAGGAGAACAAAACATTCTTTATAAGGGTGTAGAAATCTCTTTCTAGCAGACGCATTTTAGAAACCTTGAGGTAGCTCGAAAGGGAAAGCCCAAAGAGAACAATATATGCTGACGGTGGGCTTTGGCGGTTACAAATGGTATCAGAGCCAGACACCGGACGATGTGCCAGCGAGGAGGTTGAGCCTCGAAGCGGGGTGAACATGAGGTGGTGTGCCAACAAGGACACTGGACCCCGAAGGGGGTAGGTTAGGGGGTCCCACATCCATTAGAGATGGGAACAAGTGCCAGCGAGGACATTGGGCCTCGAAGGGGGTGGATTGTGAGATCCCACATCGATTGGGGAGGTAGAAGGTAGATTGTGAGATCTCATATTGGTTGGGAAGGAGAACGAAACATTCACTATTAGGGTGTAGAAACCTCTTCCTAGCAGACACGTTTTAAAAACCTTGACGGGAAGCCTGAAAGGGAAACCCCTTTTGTTACGATGTGAACAGTGCAAAGTGTATTTTTTGGAGAGAGATTCCGAAATTCATTAGGAATGTCGTCACTGACTTCAGTTCTCGTGTTCGTTGTGCAGTGAAGTATTTAAGCAAGCTAACACTAACACTAACACTAACGCTTTTTCTCATCACGTGTTTCAGTTGATACCAGATGATATGGAGTTCCAGTCCGGAGATATGCCAAACTATACCACTTCGGATGGATCAGTATATCTCTATTTGACATGCATTCTCTAACATAACGCTCTCTTGTCGTGTTTTTACTGATTTATTTCTGCTCTCGTGTAGGTTAAGATTCAGAAGGACAGTGAAGTTCGATTAAAGATAATCGGGACTCGAGTCGATGCAACTGAAATCGTAACGTCTTTTCTCTATTTAACATCGTTAAAGATAACTCTATGTTTGTTGGTTCTTGTGAGTTTGGCGTCTCATTTAACTATTGCTTTTGCTTGCAGTTTTGTATTGGAACCATTAAAGATGACTTCTTGGGTGTCATAAATGACCCTGCGGCAGCTTAG

mRNA sequence

CACTTTGTCCCGCCGCCGCCGCCTCCCTACCAGTACAGGGAGCCACTATGTTCTTCCACATAGTTTTGGAGAGGAACATGCAGCTGCATCCACGGCACTTTGGCCGTAATCTCCGTGAAAATCTGGTGTCCAAACTCATGAAAGATGTTGAGGGTACTTGCAGTGGTCGACATGGTTTCGTAGTAGCAATTACGGGAATTGAGAACATCGGTAAAGGATTGATTAGCGATGGGACAGGTTTCGTCACTTTTCCCGTTAAATACCAATGCGTCGTGTTTCGACCATTCAAGGGAGAAATCTTAGAAGCAATTGTTACTATGGTAAACAAGGTTAAGATTCAGAAGGACAGTGAAGTTCGATTAAAGATAATCGGGACTCGAGTCGATGCAACTGAAATCTTTTGTATTGGAACCATTAAAGATGACTTCTTGGGTGTCATAAATGACCCTGCGGCAGCTTAG

Coding sequence (CDS)

ATGTTCTTCCACATAGTTTTGGAGAGGAACATGCAGCTGCATCCACGGCACTTTGGCCGTAATCTCCGTGAAAATCTGGTGTCCAAACTCATGAAAGATGTTGAGGGTACTTGCAGTGGTCGACATGGTTTCGTAGTAGCAATTACGGGAATTGAGAACATCGGTAAAGGATTGATTAGCGATGGGACAGGTTTCGTCACTTTTCCCGTTAAATACCAATGCGTCGTGTTTCGACCATTCAAGGGAGAAATCTTAGAAGCAATTGTTACTATGGTAAACAAGGTTAAGATTCAGAAGGACAGTGAAGTTCGATTAAAGATAATCGGGACTCGAGTCGATGCAACTGAAATCTTTTGTATTGGAACCATTAAAGATGACTTCTTGGGTGTCATAAATGACCCTGCGGCAGCTTAG

Protein sequence

MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLISDGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA

Homology

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BLAST of Carg01422 vs. NCBI nr
Match: KAG7016596.1 (hypothetical protein SDJN02_21706 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 276.9 bits (707), Expect = 9.1e-71
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKVKIQKDSEVRLKIIGTRVDATEIFCI 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKVKIQKDSEVRLKIIGTRVDATEIFCI
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKVKIQKDSEVRLKIIGTRVDATEIFCI 120

Query: 121 GTIKDDFLGVINDPAAA 138
           GTIKDDFLGVINDPAAA
Sbjct: 121 GTIKDDFLGVINDPAAA 137

[Close]

BLAST of Carg01422 vs. NCBI nr
Match: XP_031742311.1 (DNA-directed RNA polymerase II subunit RPB7 isoform X2 [Cucumis sativus])

HSP 1 Score: 260.8 bits (665), Expect = 6.8e-66
Identity = 134/159 (84.28%), Postives = 136/159 (85.53%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLI 
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK----------------------VKIQ 120
           DGTGFVTFPVKYQCVVFRPFKGEILEA+VTMVNK                      VKIQ
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKLIPDDMEFQSGDVPNYTTSDGSVKIQ 120

Query: 121 KDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
           KDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDP+AA
Sbjct: 121 KDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPSAA 159

[Close]

BLAST of Carg01422 vs. NCBI nr
Match: TYJ20599.1 (hypothetical protein E1A91_A08G005700v1 [Gossypium mustelinum])

HSP 1 Score: 258.8 bits (660), Expect = 2.6e-65
Identity = 132/154 (85.71%), Postives = 133/154 (86.36%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIEN+GKGLI 
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENVGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-----------------VKIQKDSEV 120
           DGTGFVTFPVKYQCVVFRPFKGEILEA VTMVNK                 VKIQKDSEV
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAAVTMVNKMGFFAEAGPVQIFVSNHVKIQKDSEV 120

Query: 121 RLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
           RLKIIGTRVDATEIFCIGTIKDDFLGVINDP  A
Sbjct: 121 RLKIIGTRVDATEIFCIGTIKDDFLGVINDPTTA 154

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BLAST of Carg01422 vs. NCBI nr
Match: XP_021642607.1 (DNA-directed RNA polymerase II subunit RPB7 isoform X2 [Hevea brasiliensis])

HSP 1 Score: 258.8 bits (660), Expect = 2.6e-65
Identity = 132/154 (85.71%), Postives = 135/154 (87.66%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPR+FGRNLR+NLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLI 
Sbjct: 1   MFFHIVLERNMQLHPRYFGRNLRDNLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-----------------VKIQKDSEV 120
           DGTGFVTFPVKYQCVVFRPFKGEILEA+VTMVNK                 VKIQKDSEV
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHVKIQKDSEV 120

Query: 121 RLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
           RLKIIGTRVDATEIFCIGTIKDDFLGVINDPA A
Sbjct: 121 RLKIIGTRVDATEIFCIGTIKDDFLGVINDPATA 154

[Close]

BLAST of Carg01422 vs. NCBI nr
Match: XP_022939214.1 (DNA-directed RNA polymerase II subunit RPB7 isoform X1 [Cucurbita moschata])

HSP 1 Score: 257.7 bits (657), Expect = 5.7e-65
Identity = 137/176 (77.84%), Postives = 137/176 (77.84%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS
Sbjct: 73  MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 132

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-------------------------- 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK                          
Sbjct: 133 DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 192

Query: 121 -------------VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
                        VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA
Sbjct: 193 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 248

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BLAST of Carg01422 vs. ExPASy Swiss-Prot
Match: P46279 (DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 5.9e-65
Identity = 130/174 (74.71%), Postives = 134/174 (77.01%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPR+FGRNLR+NLVSKLMKDVEGTCSGRHGFVVA+TGIENIGKGLI 
Sbjct: 1   MFFHIVLERNMQLHPRYFGRNLRDNLVSKLMKDVEGTCSGRHGFVVAVTGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-------------------------- 120
           DGTGFVTFPVKYQCVVFRPFKGEILEA+VTMVNK                          
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 -------------VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPA 136
                        VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPA
Sbjct: 121 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPA 174

[Close]

BLAST of Carg01422 vs. ExPASy Swiss-Prot
Match: P38421 (DNA-directed RNA polymerase II subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPB7 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 1.6e-62
Identity = 126/176 (71.59%), Postives = 132/176 (75.00%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPR FGRNL+ENLVSKLMKDVEGTCSGRHGFVVAITGI+ IGKGLI 
Sbjct: 1   MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-------------------------- 120
           DGTGFVTFPVKYQCVVFRPFKGEILEA+VT+VNK                          
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQ 120

Query: 121 -------------VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
                        VKIQK+ EVRLKIIGTRVDAT IFC+GTIKDDFLGVINDPAAA
Sbjct: 121 AGDMPNYTTSDGSVKIQKECEVRLKIIGTRVDATAIFCVGTIKDDFLGVINDPAAA 176

[Close]

BLAST of Carg01422 vs. ExPASy Swiss-Prot
Match: Q5E9B8 (DNA-directed RNA polymerase II subunit RPB7 OS=Bos taurus OX=9913 GN=POLR2G PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 6.9e-37
Identity = 77/172 (44.77%), Postives = 106/172 (61.63%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MF+HI LE  + LHPR+FG NL   +  KL  +VEGTC+G++GFV+A+T I+NIG G+I 
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKV------------------------- 120
            G GFV +PVKY+ +VFRPFKGE+++A+VT VNKV                         
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 121 ---------------KIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVIN 133
                           IQ+D E+RLKI+GTRVD  +IF IG++ DD+LG+++
Sbjct: 121 PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS 172

[Close]

BLAST of Carg01422 vs. ExPASy Swiss-Prot
Match: Q7ZW41 (DNA-directed RNA polymerase II subunit RPB7 OS=Danio rerio OX=7955 GN=polr2g PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 6.9e-37
Identity = 77/172 (44.77%), Postives = 106/172 (61.63%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MF+HI LE  + LHPR+FG NL   +  KL  +VEGTC+G++GFV+A+T I+NIG G+I 
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-------------------------- 120
            G GFV +PVKY+ +VFRPFKGE+++A+VT VNK                          
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 121 --------------VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVIN 133
                         V IQ+D E+RLKI+GTRVD  +IF IG++ DD+LG+++
Sbjct: 121 PNSNPPCYKTVDEDVVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS 172

[Close]

BLAST of Carg01422 vs. ExPASy Swiss-Prot
Match: P62487 (DNA-directed RNA polymerase II subunit RPB7 OS=Homo sapiens OX=9606 GN=POLR2G PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 6.9e-37
Identity = 77/172 (44.77%), Postives = 106/172 (61.63%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MF+HI LE  + LHPR+FG NL   +  KL  +VEGTC+G++GFV+A+T I+NIG G+I 
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKV------------------------- 120
            G GFV +PVKY+ +VFRPFKGE+++A+VT VNKV                         
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 121 ---------------KIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVIN 133
                           IQ+D E+RLKI+GTRVD  +IF IG++ DD+LG+++
Sbjct: 121 PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS 172

[Close]

BLAST of Carg01422 vs. ExPASy TrEMBL
Match: A0A5D2Y397 (Uncharacterized protein OS=Gossypium mustelinum OX=34275 GN=E1A91_A08G005700v1 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.2e-65
Identity = 132/154 (85.71%), Postives = 133/154 (86.36%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIEN+GKGLI 
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENVGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-----------------VKIQKDSEV 120
           DGTGFVTFPVKYQCVVFRPFKGEILEA VTMVNK                 VKIQKDSEV
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAAVTMVNKMGFFAEAGPVQIFVSNHVKIQKDSEV 120

Query: 121 RLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
           RLKIIGTRVDATEIFCIGTIKDDFLGVINDP  A
Sbjct: 121 RLKIIGTRVDATEIFCIGTIKDDFLGVINDPTTA 154

[Close]

BLAST of Carg01422 vs. ExPASy TrEMBL
Match: A0A6J1FGH6 (DNA-directed RNA polymerase II subunit RPB7 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445199 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.8e-65
Identity = 137/176 (77.84%), Postives = 137/176 (77.84%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS
Sbjct: 73  MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 132

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-------------------------- 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK                          
Sbjct: 133 DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 192

Query: 121 -------------VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
                        VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA
Sbjct: 193 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 248

[Close]

BLAST of Carg01422 vs. ExPASy TrEMBL
Match: A0A6J1FFB3 (DNA-directed RNA polymerase II subunit RPB7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111445199 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.8e-65
Identity = 137/176 (77.84%), Postives = 137/176 (77.84%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-------------------------- 120
           DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK                          
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 -------------VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
                        VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA
Sbjct: 121 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 176

[Close]

BLAST of Carg01422 vs. ExPASy TrEMBL
Match: A0A2N9FXE3 (SHS2_Rpb7-N domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS19800 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.1e-64
Identity = 130/159 (81.76%), Postives = 135/159 (84.91%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPRHFGRNLR+N+VSKLMKDVEGTCSGRHGFVVAITGIENIGKGLI 
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRDNIVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK----------------------VKIQ 120
           DGTGFV+FPVKYQCVVFRPFKGEILEA+VTMVNK                      VKIQ
Sbjct: 61  DGTGFVSFPVKYQCVVFRPFKGEILEAVVTMVNKLIPDDMEFQSGDMPNYTTSDGSVKIQ 120

Query: 121 KDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
           KDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDP+ A
Sbjct: 121 KDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPSGA 159

[Close]

BLAST of Carg01422 vs. ExPASy TrEMBL
Match: A0A2I4HHD1 (DNA-directed RNA polymerase II subunit RPB7 OS=Juglans regia OX=51240 GN=LOC109017848 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.8e-64
Identity = 134/176 (76.14%), Postives = 136/176 (77.27%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIEN+GKGLI 
Sbjct: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENVGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-------------------------- 120
           DGTGFVTFPVKYQCVVFRPFKGEILEA+VTMVNK                          
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 121 -------------VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
                        VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA
Sbjct: 121 SGDMPNYTTSDGSVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 176

[Close]

BLAST of Carg01422 vs. TAIR 10
Match: AT5G59180.1 (DNA-directed RNA polymerase II )

HSP 1 Score: 240.0 bits (611), Expect = 1.2e-63
Identity = 126/176 (71.59%), Postives = 132/176 (75.00%), Query Frame = 0


Query: 1   MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
           MFFHIVLERNMQLHPR FGRNL+ENLVSKLMKDVEGTCSGRHGFVVAITGI+ IGKGLI 
Sbjct: 1   MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIR 60

Query: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK-------------------------- 120
           DGTGFVTFPVKYQCVVFRPFKGEILEA+VT+VNK                          
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQ 120

Query: 121 -------------VKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVINDPAAA 138
                        VKIQK+ EVRLKIIGTRVDAT IFC+GTIKDDFLGVINDPAAA
Sbjct: 121 AGDMPNYTTSDGSVKIQKECEVRLKIIGTRVDATAIFCVGTIKDDFLGVINDPAAA 176

[Close]

BLAST of Carg01422 vs. TAIR 10
Match: AT3G22900.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 49.7 bits (117), Expect = 2.2e-06
Identity = 22/55 (40.00%), Postives = 36/55 (65.45%), Query Frame = 0


Query: 43  GFVVAITGIENIGKGLISDGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNKVKI 98
           G+++  T +ENIG+G I + TG + FPV +  + F+ FKGEI+  +V  V+K  +
Sbjct: 46  GYLITPTILENIGEGKIKEQTGEIQFPVVFNGICFKMFKGEIVHGVVHKVHKTGV 100

[Close]

BLAST of Carg01422 vs. TAIR 10
Match: AT4G14660.1 (RNA polymerase Rpb7-like, N-terminal domain )

HSP 1 Score: 48.9 bits (115), Expect = 3.8e-06
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 43 GFVVAITGIENIGKGLISDGTGFVTFPVKYQCVVFRPFKGEILEAIVTMVNK 95
          G+ VA+T ++ IG+G I + TG V FPV +  + F+ FKGEI+  +V  V K
Sbjct: 47 GYYVAVTTLDKIGEGKIREHTGEVLFPVMFSGMTFKIFKGEIIHGVVHKVLK 98

[Close]

BLAST of Carg01422 vs. TAIR 10
Match: AT1G06790.2 (RNA polymerase Rpb7 N-terminal domain-containing protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.6e-04
Identity = 26/87 (29.89%), Postives = 42/87 (48.28%), Query Frame = 0


Query: 1  MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
          MF+   LE ++++ P      L + + S L            G  V+I  I+++  G + 
Sbjct: 1  MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60

Query: 61 DGTGFVTFPVKYQCVVFRPFKGEILEA 88
           G G  T+ V  + VVFRPF GE++ A
Sbjct: 61 PGDGAATYKVGLRIVVFRPFVGEVIAA 87

[Close]

BLAST of Carg01422 vs. TAIR 10
Match: AT1G06790.1 (RNA polymerase Rpb7 N-terminal domain-containing protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.6e-04
Identity = 26/87 (29.89%), Postives = 42/87 (48.28%), Query Frame = 0


Query: 1  MFFHIVLERNMQLHPRHFGRNLRENLVSKLMKDVEGTCSGRHGFVVAITGIENIGKGLIS 60
          MF+   LE ++++ P      L + + S L            G  V+I  I+++  G + 
Sbjct: 1  MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60

Query: 61 DGTGFVTFPVKYQCVVFRPFKGEILEA 88
           G G  T+ V  + VVFRPF GE++ A
Sbjct: 61 PGDGAATYKVGLRIVVFRPFVGEVIAA 87

The following BLAST results are available for this feature:

BLAST of Carg01422 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016596.1	9.1e-71	100.00	hypothetical protein SDJN02_21706 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_031742311.1	6.8e-66	84.28	DNA-directed RNA polymerase II subunit RPB7 isoform X2 [Cucumis sativus]	[more]
TYJ20599.1	2.6e-65	85.71	hypothetical protein E1A91_A08G005700v1 [Gossypium mustelinum]	[more]
XP_021642607.1	2.6e-65	85.71	DNA-directed RNA polymerase II subunit RPB7 isoform X2 [Hevea brasiliensis]	[more]
XP_022939214.1	5.7e-65	77.84	DNA-directed RNA polymerase II subunit RPB7 isoform X1 [Cucurbita moschata]	[more]

BLAST of Carg01422 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P46279	5.9e-65	74.71	DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max OX=3847 PE=2 SV=1	[more]
P38421	1.6e-62	71.59	DNA-directed RNA polymerase II subunit 7 OS=Arabidopsis thaliana OX=3702 GN=NRPB...	[more]
Q5E9B8	6.9e-37	44.77	DNA-directed RNA polymerase II subunit RPB7 OS=Bos taurus OX=9913 GN=POLR2G PE=1...	[more]
Q7ZW41	6.9e-37	44.77	DNA-directed RNA polymerase II subunit RPB7 OS=Danio rerio OX=7955 GN=polr2g PE=...	[more]
P62487	6.9e-37	44.77	DNA-directed RNA polymerase II subunit RPB7 OS=Homo sapiens OX=9606 GN=POLR2G PE...	[more]

BLAST of Carg01422 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D2Y397	1.2e-65	85.71	Uncharacterized protein OS=Gossypium mustelinum OX=34275 GN=E1A91_A08G005700v1 P...	[more]
A0A6J1FGH6	2.8e-65	77.84	DNA-directed RNA polymerase II subunit RPB7 isoform X1 OS=Cucurbita moschata OX=...	[more]
A0A6J1FFB3	2.8e-65	77.84	DNA-directed RNA polymerase II subunit RPB7 isoform X3 OS=Cucurbita moschata OX=...	[more]
A0A2N9FXE3	1.1e-64	81.76	SHS2_Rpb7-N domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS19...	[more]
A0A2I4HHD1	1.8e-64	76.14	DNA-directed RNA polymerase II subunit RPB7 OS=Juglans regia OX=51240 GN=LOC1090...	[more]

BLAST of Carg01422 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59180.1	1.2e-63	71.59	DNA-directed RNA polymerase II	[more]
AT3G22900.1	2.2e-06	40.00	RNA polymerase Rpb7-like, N-terminal domain	[more]
AT4G14660.1	3.8e-06	44.23	RNA polymerase Rpb7-like, N-terminal domain	[more]
AT1G06790.2	1.6e-04	29.89	RNA polymerase Rpb7 N-terminal domain-containing protein	[more]
AT1G06790.1	1.6e-04	29.89	RNA polymerase Rpb7 N-terminal domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.40.50.140		coord: 93..132 e-value: 6.1E-13 score: 50.6
None	No IPR available	PANTHER	PTHR12709:SF8	BNAA10G12180D PROTEIN	coord: 1..95 coord: 95..137
None	No IPR available	CDD	cd04329	RNAP_II_Rpb7_N	coord: 2..81 e-value: 3.42405E-39 score: 124.987
IPR005576	RNA polymerase Rpb7-like , N-terminal	PFAM	PF03876	SHS2_Rpb7-N	coord: 8..64 e-value: 9.9E-13 score: 48.2
IPR036898	RNA polymerase Rpb7-like, N-terminal domain superfamily	GENE3D	3.30.1490.120		coord: 1..80 e-value: 2.8E-28 score: 100.0
IPR036898	RNA polymerase Rpb7-like, N-terminal domain superfamily	SUPERFAMILY	88798	N-terminal, heterodimerisation domain of RBP7 (RpoE)	coord: 1..78
IPR045113	RNA polymerase Rpb7-like	PANTHER	PTHR12709	DNA-DIRECTED RNA POLYMERASE II, III	coord: 1..95 coord: 95..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01422-RA	Carg01422-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000291	nuclear-transcribed mRNA catabolic process, exonucleolytic
biological_process	GO:0060213	positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
biological_process	GO:0045948	positive regulation of translational initiation
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0006352	DNA-templated transcription, initiation
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0000932	P-body
cellular_component	GO:0005665	RNA polymerase II, core complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0003697	single-stranded DNA binding
molecular_function	GO:0003727	single-stranded RNA binding
molecular_function	GO:0031369	translation initiation factor binding