Carg01348 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01348
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pectinesterase
Location	Carg_Chr15: 4568998 .. 4570026 (-)
RNA-Seq Expression	Carg01348
Synteny	Carg01348

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCGAGAAGGTGGTCATGACGGGCATGTCCCTCATGCTCGTCGTCGGCGTAGCTATGGCGGTCATCGCCGTCGTTAACAAGAACAACAGTTCCGATTCTATGGTGGAGAATCTGTCCCCAAAGATGAAGGTTGTCTCATCCCTTTGTTCTTCTACAAATTATCAAAACGAATGTCAAAACACACTCGTCAGCACCGCTCATAATGCCTCAGCAAGTGACCCTAAGGCGTATGTTAAAGCCGCCATTCTTGCTACTGTTGAAGAGATTAAAAAGGGGTTTAACTTGTCTAATAACCTTCTGGTGGAGGCTGCTAACAACGCCACCATCAAAATGAGTCTCGAGGATTGCAAGGACTTATTGCAATACGCCATTGATGAGCTACATGCTTCTTACTCCACTGTCGGCGACCCTGAATTACACACCAATGAGGATCGCATAGCTGATATCAAGAACTGGCTCACGGCTGTCATTTCTTATCAGCAATCTTGTCTTGATGGTCTTGAGGAATTCGATCCCCAACTCCGAAAGAAGATGCAAGACACTCTTGATGTTGCTGGCAGGCTCACCAGCAATGCTCTGGCTCTTGTTGATGCTGTTTCCAGCATTCTTGCAAGATACGGCTTGCAATTTAAGATTAAACCAAGTGGGCGCAGGCTTCTTGGAGCAACTGAAATAGGGAGTGATGGGTTCCCGACATGGCTGACGAGGGCAGACAGGAAGCTGCTGGCAGCTAGAGGAGGTGGCGGCGGCAGAGCGAAGCCGAACGCTGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAGAGCATTGCTGCTGCGTTGGCTGCATACCCAAAAGGCTCAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAATGAATATATCATTGTGCCTAAAGAGATGAGGAACGTTTTCATGTATGGAGATGGCCCGAGAAAAACCCTTGTCACTGGCCGGAAATGCTACCGTGATGGCATCACCACCCAAGACTCCGCCTCTTTCTGTAAGTTTTTGCCATGA

mRNA sequence

ATGGTCGAGAAGGTGGTCATGACGGGCATGTCCCTCATGCTCGTCGTCGGCGTAGCTATGGCGGTCATCGCCGTCGTTAACAAGAACAACAGTTCCGATTCTATGGTGGAGAATCTGTCCCCAAAGATGAAGGTTGTCTCATCCCTTTGTTCTTCTACAAATTATCAAAACGAATGTCAAAACACACTCGTCAGCACCGCTCATAATGCCTCAGCAAGTGACCCTAAGGCGTATGTTAAAGCCGCCATTCTTGCTACTGTTGAAGAGATTAAAAAGGGGTTTAACTTGTCTAATAACCTTCTGGTGGAGGCTGCTAACAACGCCACCATCAAAATGAGTCTCGAGGATTGCAAGGACTTATTGCAATACGCCATTGATGAGCTACATGCTTCTTACTCCACTGTCGGCGACCCTGAATTACACACCAATGAGGATCGCATAGCTGATATCAAGAACTGGCTCACGGCTGTCATTTCTTATCAGCAATCTTGTCTTGATGGTCTTGAGGAATTCGATCCCCAACTCCGAAAGAAGATGCAAGACACTCTTGATGTTGCTGGCAGGCTCACCAGCAATGCTCTGGCTCTTGTTGATGCTGTTTCCAGCATTCTTGCAAGATACGGCTTGCAATTTAAGATTAAACCAAGTGGGCGCAGGCTTCTTGGAGCAACTGAAATAGGGAGTGATGGGTTCCCGACATGGCTGACGAGGGCAGACAGGAAGCTGCTGGCAGCTAGAGGAGGTGGCGGCGGCAGAGCGAAGCCGAACGCTGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAGAGCATTGCTGCTGCGTTGGCTGCATACCCAAAAGGCTCAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAATGAATATATCATTGTGCCTAAAGAGATGAGGAACGTTTTCATGTATGGAGATGGCCCGAGAAAAACCCTTGTCACTGGCCGGAAATGCTACCGTGATGGCATCACCACCCAAGACTCCGCCTCTTTCTGTAAGTTTTTGCCATGA

Coding sequence (CDS)

ATGGTCGAGAAGGTGGTCATGACGGGCATGTCCCTCATGCTCGTCGTCGGCGTAGCTATGGCGGTCATCGCCGTCGTTAACAAGAACAACAGTTCCGATTCTATGGTGGAGAATCTGTCCCCAAAGATGAAGGTTGTCTCATCCCTTTGTTCTTCTACAAATTATCAAAACGAATGTCAAAACACACTCGTCAGCACCGCTCATAATGCCTCAGCAAGTGACCCTAAGGCGTATGTTAAAGCCGCCATTCTTGCTACTGTTGAAGAGATTAAAAAGGGGTTTAACTTGTCTAATAACCTTCTGGTGGAGGCTGCTAACAACGCCACCATCAAAATGAGTCTCGAGGATTGCAAGGACTTATTGCAATACGCCATTGATGAGCTACATGCTTCTTACTCCACTGTCGGCGACCCTGAATTACACACCAATGAGGATCGCATAGCTGATATCAAGAACTGGCTCACGGCTGTCATTTCTTATCAGCAATCTTGTCTTGATGGTCTTGAGGAATTCGATCCCCAACTCCGAAAGAAGATGCAAGACACTCTTGATGTTGCTGGCAGGCTCACCAGCAATGCTCTGGCTCTTGTTGATGCTGTTTCCAGCATTCTTGCAAGATACGGCTTGCAATTTAAGATTAAACCAAGTGGGCGCAGGCTTCTTGGAGCAACTGAAATAGGGAGTGATGGGTTCCCGACATGGCTGACGAGGGCAGACAGGAAGCTGCTGGCAGCTAGAGGAGGTGGCGGCGGCAGAGCGAAGCCGAACGCTGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAGAGCATTGCTGCTGCGTTGGCTGCATACCCAAAAGGCTCAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAATGAATATATCATTGTGCCTAAAGAGATGAGGAACGTTTTCATGTATGGAGATGGCCCGAGAAAAACCCTTGTCACTGGCCGGAAATGCTACCGTGATGGCATCACCACCCAAGACTCCGCCTCTTTCTGTAAGTTTTTGCCATGA

Protein sequence

MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQNTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDLLQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQDTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADRKLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIVPKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASFCKFLP

Homology

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BLAST of Carg01348 vs. NCBI nr
Match: KAG7016670.1 (putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 659.8 bits (1701), Expect = 1.2e-185
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ
Sbjct: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
           NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL
Sbjct: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ
Sbjct: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR
Sbjct: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV
Sbjct: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASFCKFLP 343
           PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASFCKFLP
Sbjct: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASFCKFLP 342

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BLAST of Carg01348 vs. NCBI nr
Match: KAG6579152.1 (putative pectinesterase/pectinesterase inhibitor 45, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 647.5 bits (1669), Expect = 6.4e-182
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ
Sbjct: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
           NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL
Sbjct: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ
Sbjct: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR
Sbjct: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV
Sbjct: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 338
           PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF
Sbjct: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 337

[Close]

BLAST of Carg01348 vs. NCBI nr
Match: XP_022938856.1 (pectinesterase [Cucurbita moschata])

HSP 1 Score: 644.0 bits (1660), Expect = 7.1e-181
Identity = 335/337 (99.41%), Postives = 336/337 (99.70%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ
Sbjct: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
           NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL
Sbjct: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDP LRKKMQ
Sbjct: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPHLRKKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWL+RADR
Sbjct: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLSRADR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV
Sbjct: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 338
           PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF
Sbjct: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 337

[Close]

BLAST of Carg01348 vs. NCBI nr
Match: XP_023549872.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630.2 bits (1624), Expect = 1.1e-176
Identity = 328/337 (97.33%), Postives = 331/337 (98.22%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MVEKVV+TGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ
Sbjct: 1   MVEKVVVTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
           NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNL+VEAANNATIKMSLEDCKDL
Sbjct: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLMVEAANNATIKMSLEDCKDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQYAIDELHASYSTVGDPELHT EDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ
Sbjct: 121 LQYAIDELHASYSTVGDPELHTKEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           DTLD AGRLTSNALALVDAVSSIL  YGLQFKIKPSGRRLLGATEIGSDGFPTWL+R DR
Sbjct: 181 DTLDFAGRLTSNALALVDAVSSILESYGLQFKIKPSGRRLLGATEIGSDGFPTWLSRTDR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV
Sbjct: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 338
           PKEMRNVFMYGDGPRKTLVTGRKC RDGITTQDSASF
Sbjct: 301 PKEMRNVFMYGDGPRKTLVTGRKCNRDGITTQDSASF 337

[Close]

BLAST of Carg01348 vs. NCBI nr
Match: XP_022993420.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 623.2 bits (1606), Expect = 1.3e-174
Identity = 325/337 (96.44%), Postives = 329/337 (97.63%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MVEKVV+TGMSLMLVVGVAMAVIAVVNKN SSDSMVENLSPKMKVVSSLCSSTNYQNECQ
Sbjct: 1   MVEKVVVTGMSLMLVVGVAMAVIAVVNKNKSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
           NTL STAHNASASDPKAYVKAAILATVEEIKKGFNLSNNL+VEAANNATIKMSLEDCKDL
Sbjct: 61  NTLGSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLMVEAANNATIKMSLEDCKDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQYAIDELHASYSTVGDPELHT EDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ
Sbjct: 121 LQYAIDELHASYSTVGDPELHTKEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           DTLD AGRLTSNALALVDAVSSIL RYGLQFKIKPSGRRLLGATEIGSDGFPTWL+R DR
Sbjct: 181 DTLDFAGRLTSNALALVDAVSSILERYGLQFKIKPSGRRLLGATEIGSDGFPTWLSRTDR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV
Sbjct: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 338
            KEMRNVFMYGDGPRKTLVTGRKC RDGITTQD+ASF
Sbjct: 301 TKEMRNVFMYGDGPRKTLVTGRKCNRDGITTQDTASF 337

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BLAST of Carg01348 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.3e-49
Identity = 134/372 (36.02%), Postives = 203/372 (54.57%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           M+ KVV++  S++L+VGVA+ V+A +NKN  +     NLSP+MK V  +C ST+ +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASC- 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNAT--IKMSLEDCK 120
              V T     + DP   +KA +LAT +E+ K  N +    V   ++ +   K  L+ CK
Sbjct: 61  ---VKTLEPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKK 180
            +  YA+++L      +G+ +L     +I  +K WL  V +YQ  CLD +EE D  LRK 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDTLDVAGRLTSNALALVDAVSSILARYGLQFK-----------------------IKP 240
           + + +  +  LT+NA+ +   V S +A+   +                           P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SG--RRLLGATEIGSDGFPTWLTRADRKLLAARG-------GGGGRAKPNAVVAKDGSGQ 300
            G  RRLL   +I   G PTW++ ADRKL+A  G       GGG R + N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIVPKEMRNVFMYGDGPRKTLVTGRK--C 337
           FK++  A+ A P+ ++GR +IY+KAG+Y E +I+PK+  N+FM+GDG RKT+++  +   
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 358

[Close]

BLAST of Carg01348 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.8e-47
Identity = 115/334 (34.43%), Postives = 192/334 (57.49%), Query Frame = 0


Query: 3   EKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQNT 62
           ++ V+  +S +L++ + +AV   V+ N S ++  E ++  +K +  +C+ T+Y+  C++T
Sbjct: 14  KRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDT 73

Query: 63  LVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDLLQ 122
           L   A +   SDP   VK A  AT+++I      S   ++E   +   KM+L+ CK+L+ 
Sbjct: 74  LRKDAKD--TSDPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMD 133

Query: 123 YAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQDT 182
           YAI EL  S+  +G  E H  ++ +  ++ WL+A IS++Q+CLDG +       + ++  
Sbjct: 134 YAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 193

Query: 183 LDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADRKL 242
           L  A +LT N LA+V  +S+ L +  +    + + RRLL      S  FP+W+    R+L
Sbjct: 194 LKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRL 253

Query: 243 LAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIVPK 302
           L A        KP+ VVA+DGSGQ+K+I  AL   PK     +V+++K GIY EY+ V +
Sbjct: 254 LNA---PMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNR 313

Query: 303 EMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSAS 337
            M ++   GDGP KT+++G K Y+DGITT  +A+
Sbjct: 314 SMTHLVFIGDGPDKTVISGSKSYKDGITTYKTAT 332

[Close]

BLAST of Carg01348 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 189.1 bits (479), Expect = 8.2e-47
Identity = 112/333 (33.63%), Postives = 194/333 (58.26%), Query Frame = 0


Query: 5   VVMTGMSLMLV-VGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQNTL 64
           +V+T  S++L+ + VA+ V   +NK++        ++  +K V  +C+ T+Y+  C++TL
Sbjct: 16  IVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL 75

Query: 65  VSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDLLQY 124
           +    N   +DP   VK A   T+++I      S  ++ E   ++  +M+L+ CK+L+ Y
Sbjct: 76  IKNGKN--TTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDY 135

Query: 125 AIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQDTL 184
           A+DEL  S+  +G  E H  ++ + +++ WL+A IS++++CL+G +       + M+  L
Sbjct: 136 ALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKAL 195

Query: 185 DVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADRKLL 244
             A  LT N LA++  +S+ + +  +      + RRLL      ++GFP+W+ +  RKLL
Sbjct: 196 KTAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLL------AEGFPSWVDQRGRKLL 255

Query: 245 AARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIVPKE 304
            A        KP+ VVA+DGSGQ+K+I  AL   PK     +V+++KAG+Y EY+ V K 
Sbjct: 256 QA-AAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKT 315

Query: 305 MRNVFMYGDGPRKTLVTGRKCYRDGITTQDSAS 337
           M ++   GDGP KT+++G K Y+DGITT  +A+
Sbjct: 316 MSHLVFIGDGPDKTIISGNKNYKDGITTYRTAT 335

[Close]

BLAST of Carg01348 vs. ExPASy Swiss-Prot
Match: Q5MFV6 (Probable pectinesterase/pectinesterase inhibitor VGDH2 OS=Arabidopsis thaliana OX=3702 GN=VGDH2 PE=2 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 6.9e-46
Identity = 127/347 (36.60%), Postives = 189/347 (54.47%), Query Frame = 0


Query: 2   VEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVEN-LSPKMKVVSSLCSSTNYQNECQ 61
           V KVV++  SL+LVVGVA+ VI  VNK   ++      ++   K V ++C ST  Q  C 
Sbjct: 3   VGKVVVSVASLLLVVGVAIGVITFVNKGGGANGDSNGPINSHQKAVQTICQSTTDQGSCA 62

Query: 62  NTLVSTAHNASASDPKAYVKAAILATVEEIKK--GFNLSNNLLVEAANNATIKMSLEDCK 121
            TL        + DP   VKA ++AT + I K   F  S    +    NAT K  L+ CK
Sbjct: 63  KTL----DPVKSDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCK 122

Query: 122 DLLQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKK 181
            +L YA+++L      +G+ +L  +  ++  +K WLT V +YQ  CLD +EE   +L+K 
Sbjct: 123 RVLMYALEDLETIVEEMGE-DLQQSGTKLDQLKQWLTGVFNYQTDCLDDIEEV--ELKKI 182

Query: 182 MQDTLDVAGRLTSNALALVDAVSSILARYGLQFK----------IKPSGRRLLGATEIGS 241
           M + +  +  LTSNA+ +  +V + +A+ G++               + RRLL   +  S
Sbjct: 183 MGEGISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDND--S 242

Query: 242 DGFPTWLTRADRKLLAAR---------------GGGGGRAKPNAVVAKDGSGQFKSIAAA 301
            G P W +  DRKL+A                 GGGGG+ K   VVAKDGSGQFK+I+ A
Sbjct: 243 KGLPKWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTISEA 302

Query: 302 LAAYPKGSKGRYVIYVKAGIYNEYIIVPKEMRNVFMYGDGPRKTLVT 321
           + A P  + GR +I++KAGIYNE + +PK+  N+FM+GDG  +T++T
Sbjct: 303 VMACPDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIIT 340

[Close]

BLAST of Carg01348 vs. ExPASy Swiss-Prot
Match: Q5MFV8 (Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 6.9e-46
Identity = 133/379 (35.09%), Postives = 203/379 (53.56%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           M+ KVV++  S++L+VGVA+ V+A +NKN  +     NLSP+MK V  +C +T+ +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA-----NLSPQMKAVRGICEATSDKASC- 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNL--LVEAANNATIKMSLEDCK 120
              V T     + DP   +KA +LAT + I +  N +      + +  +   K  L+ CK
Sbjct: 61  ---VKTLEPVKSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVLDYCK 120

Query: 121 DLLQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKK 180
            +  YA+++L      +G+ +L+    +I  +K WLT V +YQ  CLD +EE D  LRK 
Sbjct: 121 KVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LRKT 180

Query: 181 MQDTLDVAGRLTSNALALVDAVSSILARYGLQ---FKIKPSG------------------ 240
           + + +  +  LTSNA+ +   V S +A+  L+   FK    G                  
Sbjct: 181 IGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGTPP 240

Query: 241 --------------RRLLGATEIGSDGFPTWLTRADRKLL--AARGG--GGGRAKPNAVV 300
                         RRLL   +I   G PTW++ ADRKL+  A RG   GG R +   VV
Sbjct: 241 VADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATFVV 300

Query: 301 AKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIVPKEMRNVFMYGDGPRKTLV 337
           AKDGSGQFK++  A+ A P+ + GR +I++KAGIY E +I+PK+  N+FM+GDG RKT++
Sbjct: 301 AKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVI 360

[Close]

BLAST of Carg01348 vs. ExPASy TrEMBL
Match: A0A6J1FEB3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444938 PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.4e-181
Identity = 335/337 (99.41%), Postives = 336/337 (99.70%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ
Sbjct: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
           NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL
Sbjct: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDP LRKKMQ
Sbjct: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPHLRKKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWL+RADR
Sbjct: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLSRADR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV
Sbjct: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 338
           PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF
Sbjct: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 337

[Close]

BLAST of Carg01348 vs. ExPASy TrEMBL
Match: A0A6J1JYH0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489438 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.3e-175
Identity = 325/337 (96.44%), Postives = 329/337 (97.63%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MVEKVV+TGMSLMLVVGVAMAVIAVVNKN SSDSMVENLSPKMKVVSSLCSSTNYQNECQ
Sbjct: 1   MVEKVVVTGMSLMLVVGVAMAVIAVVNKNKSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
           NTL STAHNASASDPKAYVKAAILATVEEIKKGFNLSNNL+VEAANNATIKMSLEDCKDL
Sbjct: 61  NTLGSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLMVEAANNATIKMSLEDCKDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQYAIDELHASYSTVGDPELHT EDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ
Sbjct: 121 LQYAIDELHASYSTVGDPELHTKEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           DTLD AGRLTSNALALVDAVSSIL RYGLQFKIKPSGRRLLGATEIGSDGFPTWL+R DR
Sbjct: 181 DTLDFAGRLTSNALALVDAVSSILERYGLQFKIKPSGRRLLGATEIGSDGFPTWLSRTDR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV
Sbjct: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 338
            KEMRNVFMYGDGPRKTLVTGRKC RDGITTQD+ASF
Sbjct: 301 TKEMRNVFMYGDGPRKTLVTGRKCNRDGITTQDTASF 337

[Close]

BLAST of Carg01348 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 3.4e-136
Identity = 253/337 (75.07%), Postives = 297/337 (88.13%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MV KVVM+G+SL+LVVGVA+AV+A+VNK++SSD    NLSPKMK V+S+CS+ +YQ ECQ
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDE-TANLSPKMKAVASICSNADYQPECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
            TL S  +N+S  DPK ++KAAI +T+EE+KKG+NL++NL+VEAANNATIKMS++DCKDL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQ AIDELHASYSTVGDP+LHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQL++KMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           D LDVAG+LTSNALA+V AVS IL   GLQ K++PSGRRLLG TE+ SD FPTWLT +DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLAA+  GG R KPN VVAKDGSGQ+K+I AALAAYPK  KGRYVIYVKAG+YNE IIV
Sbjct: 241 KLLAAK-RGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIV 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 338
            K+M+N+FMYGDGPRKT+VTGRK  RDGITTQ++ASF
Sbjct: 301 TKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASF 335

[Close]

BLAST of Carg01348 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 9.8e-136
Identity = 240/337 (71.22%), Postives = 296/337 (87.83%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MV KV++ G+SL+LVVGVA+AV+A+V+ N SSD+  E+LSPKMK VS++C++ NYQ ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
           NTL + AHNAS+ DPK YV+AAIL+T+EE+KKG+NL++  ++EAANN +IKM +EDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQ+AID+L ASYSTVG+P+LHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQL++KMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           D L  AG+LTSNALA+VDAVS ILA +GLQ K +PSGRRLLG TE+G DG+PTWLT ADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLA+R GGG + KPNAVVAKDGSGQFK+I+AALAAYPK  +GRYVIYVKAGIYNEY+ +
Sbjct: 241 KLLASR-GGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHI 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 338
            K+M+N+FMYGDGP+KT+VTG K  R G TTQD+A+F
Sbjct: 301 TKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATF 336

[Close]

BLAST of Carg01348 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.2e-135
Identity = 239/337 (70.92%), Postives = 296/337 (87.83%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           MV KV++ G++L+LVVGVA+AV+A+V+ N SSD+  E+LSPKMK VS++C++ NYQ ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDL 120
           NTL + AHNAS+ DPK YV+AAIL+T+EE+KKG+NL++  ++EAANN +IKM +EDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQ 180
           LQ+AID+L ASYSTVG+P+LHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQL++KMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DTLDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADR 240
           D L  AG+LTSNALA+VDAVS ILA +GLQ K +PSGRRLLG TE+G DG+PTWLT ADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIV 300
           KLLA+R GGG + KPNAVVAKDGSGQFK+I+AALAAYPK  +GRYVIYVKAGIYNEY+ +
Sbjct: 241 KLLASR-GGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHI 300

Query: 301 PKEMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSASF 338
            K+M+N+FMYGDGP+KT+VTG K  R G TTQD+A+F
Sbjct: 301 TKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATF 336

[Close]

BLAST of Carg01348 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-50
Identity = 134/372 (36.02%), Postives = 203/372 (54.57%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           M+ KVV++  S++L+VGVA+ V+A +NKN  +     NLSP+MK V  +C ST+ +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASC- 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNAT--IKMSLEDCK 120
              V T     + DP   +KA +LAT +E+ K  N +    V   ++ +   K  L+ CK
Sbjct: 61  ---VKTLEPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKK 180
            +  YA+++L      +G+ +L     +I  +K WL  V +YQ  CLD +EE D  LRK 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDTLDVAGRLTSNALALVDAVSSILARYGLQFK-----------------------IKP 240
           + + +  +  LT+NA+ +   V S +A+   +                           P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SG--RRLLGATEIGSDGFPTWLTRADRKLLAARG-------GGGGRAKPNAVVAKDGSGQ 300
            G  RRLL   +I   G PTW++ ADRKL+A  G       GGG R + N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIVPKEMRNVFMYGDGPRKTLVTGRK--C 337
           FK++  A+ A P+ ++GR +IY+KAG+Y E +I+PK+  N+FM+GDG RKT+++  +   
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 358

[Close]

BLAST of Carg01348 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 190.7 bits (483), Expect = 2.0e-48
Identity = 115/334 (34.43%), Postives = 192/334 (57.49%), Query Frame = 0


Query: 3   EKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQNT 62
           ++ V+  +S +L++ + +AV   V+ N S ++  E ++  +K +  +C+ T+Y+  C++T
Sbjct: 14  KRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDT 73

Query: 63  LVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDLLQ 122
           L   A +   SDP   VK A  AT+++I      S   ++E   +   KM+L+ CK+L+ 
Sbjct: 74  LRKDAKD--TSDPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMD 133

Query: 123 YAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQDT 182
           YAI EL  S+  +G  E H  ++ +  ++ WL+A IS++Q+CLDG +       + ++  
Sbjct: 134 YAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 193

Query: 183 LDVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADRKL 242
           L  A +LT N LA+V  +S+ L +  +    + + RRLL      S  FP+W+    R+L
Sbjct: 194 LKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRL 253

Query: 243 LAARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIVPK 302
           L A        KP+ VVA+DGSGQ+K+I  AL   PK     +V+++K GIY EY+ V +
Sbjct: 254 LNA---PMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNR 313

Query: 303 EMRNVFMYGDGPRKTLVTGRKCYRDGITTQDSAS 337
            M ++   GDGP KT+++G K Y+DGITT  +A+
Sbjct: 314 SMTHLVFIGDGPDKTVISGSKSYKDGITTYKTAT 332

[Close]

BLAST of Carg01348 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 189.1 bits (479), Expect = 5.8e-48
Identity = 112/333 (33.63%), Postives = 194/333 (58.26%), Query Frame = 0


Query: 5   VVMTGMSLMLV-VGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQNTL 64
           +V+T  S++L+ + VA+ V   +NK++        ++  +K V  +C+ T+Y+  C++TL
Sbjct: 16  IVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL 75

Query: 65  VSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNLLVEAANNATIKMSLEDCKDLLQY 124
           +    N   +DP   VK A   T+++I      S  ++ E   ++  +M+L+ CK+L+ Y
Sbjct: 76  IKNGKN--TTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDY 135

Query: 125 AIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKKMQDTL 184
           A+DEL  S+  +G  E H  ++ + +++ WL+A IS++++CL+G +       + M+  L
Sbjct: 136 ALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKAL 195

Query: 185 DVAGRLTSNALALVDAVSSILARYGLQFKIKPSGRRLLGATEIGSDGFPTWLTRADRKLL 244
             A  LT N LA++  +S+ + +  +      + RRLL      ++GFP+W+ +  RKLL
Sbjct: 196 KTAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLL------AEGFPSWVDQRGRKLL 255

Query: 245 AARGGGGGRAKPNAVVAKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIVPKE 304
            A        KP+ VVA+DGSGQ+K+I  AL   PK     +V+++KAG+Y EY+ V K 
Sbjct: 256 QA-AAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKT 315

Query: 305 MRNVFMYGDGPRKTLVTGRKCYRDGITTQDSAS 337
           M ++   GDGP KT+++G K Y+DGITT  +A+
Sbjct: 316 MSHLVFIGDGPDKTIISGNKNYKDGITTYRTAT 335

[Close]

BLAST of Carg01348 vs. TAIR 10
Match: AT2G47040.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 186.0 bits (471), Expect = 4.9e-47
Identity = 133/379 (35.09%), Postives = 203/379 (53.56%), Query Frame = 0


Query: 1   MVEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVENLSPKMKVVSSLCSSTNYQNECQ 60
           M+ KVV++  S++L+VGVA+ V+A +NKN  +     NLSP+MK V  +C +T+ +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA-----NLSPQMKAVRGICEATSDKASC- 60

Query: 61  NTLVSTAHNASASDPKAYVKAAILATVEEIKKGFNLSNNL--LVEAANNATIKMSLEDCK 120
              V T     + DP   +KA +LAT + I +  N +      + +  +   K  L+ CK
Sbjct: 61  ---VKTLEPVKSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVLDYCK 120

Query: 121 DLLQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKK 180
            +  YA+++L      +G+ +L+    +I  +K WLT V +YQ  CLD +EE D  LRK 
Sbjct: 121 KVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LRKT 180

Query: 181 MQDTLDVAGRLTSNALALVDAVSSILARYGLQ---FKIKPSG------------------ 240
           + + +  +  LTSNA+ +   V S +A+  L+   FK    G                  
Sbjct: 181 IGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGTPP 240

Query: 241 --------------RRLLGATEIGSDGFPTWLTRADRKLL--AARGG--GGGRAKPNAVV 300
                         RRLL   +I   G PTW++ ADRKL+  A RG   GG R +   VV
Sbjct: 241 VADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATFVV 300

Query: 301 AKDGSGQFKSIAAALAAYPKGSKGRYVIYVKAGIYNEYIIVPKEMRNVFMYGDGPRKTLV 337
           AKDGSGQFK++  A+ A P+ + GR +I++KAGIY E +I+PK+  N+FM+GDG RKT++
Sbjct: 301 AKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVI 360

[Close]

BLAST of Carg01348 vs. TAIR 10
Match: AT3G62170.1 (VANGUARD 1 homolog 2 )

HSP 1 Score: 186.0 bits (471), Expect = 4.9e-47
Identity = 127/347 (36.60%), Postives = 189/347 (54.47%), Query Frame = 0


Query: 2   VEKVVMTGMSLMLVVGVAMAVIAVVNKNNSSDSMVEN-LSPKMKVVSSLCSSTNYQNECQ 61
           V KVV++  SL+LVVGVA+ VI  VNK   ++      ++   K V ++C ST  Q  C 
Sbjct: 3   VGKVVVSVASLLLVVGVAIGVITFVNKGGGANGDSNGPINSHQKAVQTICQSTTDQGSCA 62

Query: 62  NTLVSTAHNASASDPKAYVKAAILATVEEIKK--GFNLSNNLLVEAANNATIKMSLEDCK 121
            TL        + DP   VKA ++AT + I K   F  S    +    NAT K  L+ CK
Sbjct: 63  KTL----DPVKSDDPSKLVKAFLMATKDAITKSSNFTASTEGGMGTNMNATSKAVLDYCK 122

Query: 122 DLLQYAIDELHASYSTVGDPELHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLRKK 181
            +L YA+++L      +G+ +L  +  ++  +K WLT V +YQ  CLD +EE   +L+K 
Sbjct: 123 RVLMYALEDLETIVEEMGE-DLQQSGTKLDQLKQWLTGVFNYQTDCLDDIEEV--ELKKI 182

Query: 182 MQDTLDVAGRLTSNALALVDAVSSILARYGLQFK----------IKPSGRRLLGATEIGS 241
           M + +  +  LTSNA+ +  +V + +A+ G++               + RRLL   +  S
Sbjct: 183 MGEGISNSKVLTSNAIDIFHSVVTAMAQMGVKVDDMKNITMGAGAGGAARRLLEDND--S 242

Query: 242 DGFPTWLTRADRKLLAAR---------------GGGGGRAKPNAVVAKDGSGQFKSIAAA 301
            G P W +  DRKL+A                 GGGGG+ K   VVAKDGSGQFK+I+ A
Sbjct: 243 KGLPKWFSGKDRKLMAKAGRGAPAGGDDGIGEGGGGGGKIKATHVVAKDGSGQFKTISEA 302

Query: 302 LAAYPKGSKGRYVIYVKAGIYNEYIIVPKEMRNVFMYGDGPRKTLVT 321
           + A P  + GR +I++KAGIYNE + +PK+  N+FM+GDG  +T++T
Sbjct: 303 VMACPDKNPGRCIIHIKAGIYNEQVRIPKKKNNIFMFGDGATQTIIT 340

The following BLAST results are available for this feature:

BLAST of Carg01348 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016670.1	1.2e-185	100.00	putative pectinesterase/pectinesterase inhibitor 21, partial [Cucurbita argyrosp...	[more]
KAG6579152.1	6.4e-182	100.00	putative pectinesterase/pectinesterase inhibitor 45, partial [Cucurbita argyrosp...	[more]
XP_022938856.1	7.1e-181	99.41	pectinesterase [Cucurbita moschata]	[more]
XP_023549872.1	1.1e-176	97.33	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022993420.1	1.3e-174	96.44	pectinesterase-like [Cucurbita maxima]	[more]

BLAST of Carg01348 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80722	2.3e-49	36.02	Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1	[more]
Q3E8Z8	2.8e-47	34.43	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q8GX86	8.2e-47	33.63	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q5MFV6	6.9e-46	36.60	Probable pectinesterase/pectinesterase inhibitor VGDH2 OS=Arabidopsis thaliana O...	[more]
Q5MFV8	6.9e-46	35.09	Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2	[more]

BLAST of Carg01348 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FEB3	3.4e-181	99.41	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444938 PE=3 SV=1	[more]
A0A6J1JYH0	6.3e-175	96.44	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489438 PE=3 SV=1	[more]
A0A0A0KLN2	3.4e-136	75.07	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1	[more]
A0A5A7U9J8	9.8e-136	71.22	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100...	[more]
A0A1S3CR27	2.2e-135	70.92	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1	[more]

BLAST of Carg01348 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47030.1	1.6e-50	36.02	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G27870.1	2.0e-48	34.43	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05610.1	5.8e-48	33.63	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47040.1	4.9e-47	35.09	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G62170.1	4.9e-47	36.60	VANGUARD 1 homolog 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 41..196 e-value: 3.6E-32 score: 122.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 30..198 e-value: 2.4E-20 score: 70.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 45..196 e-value: 6.2E-25 score: 88.3
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 256..338 e-value: 1.9E-24 score: 85.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 251..339 e-value: 4.0E-23 score: 84.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 42..201 e-value: 3.4E-29 score: 103.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 44..200
None	No IPR available	PANTHER	PTHR31707:SF292	PECTINESTERASE	coord: 1..337
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 1..337
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 48..202 e-value: 1.72913E-44 score: 147.97
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 281..300
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 253..337

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01348-RA	Carg01348-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity