Carg01287 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01287
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	calmodulin-binding protein 25-like
Location	Carg_Chr06: 219838 .. 220485 (+)
RNA-Seq Expression	Carg01287
Synteny	Carg01287

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCCTCTGATAATCTCACAACCATCCTGGATCCATGGGCCTTCCGCCCCTCATTTCAAGACCCATGGATTTCCGATGTCTTCTCCAGAGAAACAGACGCACTCTCCAACCCCATTCACACCTCCCACTCCGACCTCTTCTCTTCCTTTTTCGACTTAATTGCCCCTGATTCTACTCCCACTCCCACGCCCACTGGTTCAGGAATCTCCGCCGACCCCGAAATCCCCGCCCCCAACCGACCCATCTCTTCGATCCCCCCCACCGGACGGAAAATCACCAAGAGGAAGTCCAGAGCCTCCAAGCGGAACCACACCACATTCATCACGGCCGACCCGGCTAACTTCCGCCACATGGTCCAGCAGGTCACCGGAGTCAGATTTGGCAACTCCCACCTTCAGATTCCTCCGGTTTCAAAGCCGGAACCGCAGAGCCCAGCTGGGCGCTTCACTGTCTGCGGCGCTGATGCTGGACTGCCTACCCTCGACACGTCGGCTTATTTGCTGAACCACCACCACCAGAACTCCGGGAGTGGATCCGAGATAACTGGGCCGGGTTTTGGGTTGTCAAATGACGCCCTTATCGGGTCGGATTTTGATACATTTTCGTCTTTTCCCACTCTCGAGTCGTGGAAGGCGGTCATGTGA

mRNA sequence

ATGGCCTCCTCTGATAATCTCACAACCATCCTGGATCCATGGGCCTTCCGCCCCTCATTTCAAGACCCATGGATTTCCGATGTCTTCTCCAGAGAAACAGACGCACTCTCCAACCCCATTCACACCTCCCACTCCGACCTCTTCTCTTCCTTTTTCGACTTAATTGCCCCTGATTCTACTCCCACTCCCACGCCCACTGGTTCAGGAATCTCCGCCGACCCCGAAATCCCCGCCCCCAACCGACCCATCTCTTCGATCCCCCCCACCGGACGGAAAATCACCAAGAGGAAGTCCAGAGCCTCCAAGCGGAACCACACCACATTCATCACGGCCGACCCGGCTAACTTCCGCCACATGGTCCAGCAGGTCACCGGAGTCAGATTTGGCAACTCCCACCTTCAGATTCCTCCGGTTTCAAAGCCGGAACCGCAGAGCCCAGCTGGGCGCTTCACTGTCTGCGGCGCTGATGCTGGACTGCCTACCCTCGACACGTCGGCTTATTTGCTGAACCACCACCACCAGAACTCCGGGAGTGGATCCGAGATAACTGGGCCGGGTTTTGGGTTGTCAAATGACGCCCTTATCGGGTCGGATTTTGATACATTTTCGTCTTTTCCCACTCTCGAGTCGTGGAAGGCGGTCATGTGA

Coding sequence (CDS)

ATGGCCTCCTCTGATAATCTCACAACCATCCTGGATCCATGGGCCTTCCGCCCCTCATTTCAAGACCCATGGATTTCCGATGTCTTCTCCAGAGAAACAGACGCACTCTCCAACCCCATTCACACCTCCCACTCCGACCTCTTCTCTTCCTTTTTCGACTTAATTGCCCCTGATTCTACTCCCACTCCCACGCCCACTGGTTCAGGAATCTCCGCCGACCCCGAAATCCCCGCCCCCAACCGACCCATCTCTTCGATCCCCCCCACCGGACGGAAAATCACCAAGAGGAAGTCCAGAGCCTCCAAGCGGAACCACACCACATTCATCACGGCCGACCCGGCTAACTTCCGCCACATGGTCCAGCAGGTCACCGGAGTCAGATTTGGCAACTCCCACCTTCAGATTCCTCCGGTTTCAAAGCCGGAACCGCAGAGCCCAGCTGGGCGCTTCACTGTCTGCGGCGCTGATGCTGGACTGCCTACCCTCGACACGTCGGCTTATTTGCTGAACCACCACCACCAGAACTCCGGGAGTGGATCCGAGATAACTGGGCCGGGTTTTGGGTTGTCAAATGACGCCCTTATCGGGTCGGATTTTGATACATTTTCGTCTTTTCCCACTCTCGAGTCGTGGAAGGCGGTCATGTGA

Protein sequence

MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDSTPTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMVQQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCGADAGLPTLDTSAYLLNHHHQNSGSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM

Homology

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BLAST of Carg01287 vs. NCBI nr
Match: KAG7027718.1 (Calmodulin-binding protein 25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 439.1 bits (1128), Expect = 2.2e-119
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60
           MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST
Sbjct: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60

Query: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120
           PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV
Sbjct: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120

Query: 121 QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCGADAGLPTLDTSAYLLNHHHQNSGSGS 180
           QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCGADAGLPTLDTSAYLLNHHHQNSGSGS
Sbjct: 121 QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCGADAGLPTLDTSAYLLNHHHQNSGSGS 180

Query: 181 EITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           EITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM
Sbjct: 181 EITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 215

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BLAST of Carg01287 vs. NCBI nr
Match: KAG6596181.1 (Calmodulin-binding protein 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 430.3 bits (1105), Expect = 1.0e-116
Identity = 214/219 (97.72%), Postives = 214/219 (97.72%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60
           MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST
Sbjct: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60

Query: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120
           PTPTPTGSGISADPEIPAPNR ISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV
Sbjct: 61  PTPTPTGSGISADPEIPAPNRSISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120

Query: 121 QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCG----ADAGLPTLDTSAYLLNHHHQNS 180
           QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCG    ADAGLPTLDTSAYLLNHHHQNS
Sbjct: 121 QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCGADADADAGLPTLDTSAYLLNHHHQNS 180

Query: 181 GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM
Sbjct: 181 GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 219

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BLAST of Carg01287 vs. NCBI nr
Match: XP_022956380.1 (calmodulin-binding protein 25-like [Cucurbita moschata])

HSP 1 Score: 410.2 bits (1053), Expect = 1.1e-110
Identity = 207/219 (94.52%), Postives = 208/219 (94.98%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60
           MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPI   HSDLFSSFFDLIAPDST
Sbjct: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPI---HSDLFSSFFDLIAPDST 60

Query: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120
           PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV
Sbjct: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120

Query: 121 QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVC----GADAGLPTLDTSAYLLNHHHQNS 180
           QQVTGVRFGNSHLQIPP+ KPEPQ PAGRFTVC     ADAGLPTLDTSAYLLNHH QNS
Sbjct: 121 QQVTGVRFGNSHLQIPPLLKPEPQRPAGRFTVCDADADADAGLPTLDTSAYLLNHHQQNS 180

Query: 181 GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM
Sbjct: 181 GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216

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BLAST of Carg01287 vs. NCBI nr
Match: XP_022971583.1 (calmodulin-binding protein 25-like [Cucurbita maxima])

HSP 1 Score: 396.7 bits (1018), Expect = 1.2e-106
Identity = 199/217 (91.71%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60
           MASSDNLTTILDPWAFRPSFQDPWISDVFSRE  ALSNPIHTSHSD+ SSFFDLIAPDST
Sbjct: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSREAHALSNPIHTSHSDVLSSFFDLIAPDST 60

Query: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120
           PTPTPT SGISAD     PNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV
Sbjct: 61  PTPTPTASGISAD-----PNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120

Query: 121 QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCG--ADAGLPTLDTSAYLLNHHHQNSGS 180
           QQVTGVRFG+SHLQIPP+ KPEPQ PAGRFTVCG  ADAGLPTLDTSAYLLNHH QNSGS
Sbjct: 121 QQVTGVRFGSSHLQIPPLLKPEPQRPAGRFTVCGADADAGLPTLDTSAYLLNHHQQNSGS 180

Query: 181 GSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           GSEITGPGFGL+NDALIGSDFDTFSSFPTLESWKAVM
Sbjct: 181 GSEITGPGFGLTNDALIGSDFDTFSSFPTLESWKAVM 212

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BLAST of Carg01287 vs. NCBI nr
Match: XP_023539162.1 (calmodulin-binding protein 25-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023539163.1 calmodulin-binding protein 25-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.8 bits (1013), Expect = 4.7e-106
Identity = 199/219 (90.87%), Postives = 202/219 (92.24%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60
           MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST
Sbjct: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60

Query: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120
           PTPTPT SGISADP+IP+PN PISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV
Sbjct: 61  PTPTPTASGISADPQIPSPNPPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120

Query: 121 QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCG----ADAGLPTLDTSAYLLNHHHQNS 180
           QQVTGVRFGNSHLQIPP+ KPEPQ PAGRFTVC     ADAGLPTLDTSAYLLNHH QNS
Sbjct: 121 QQVTGVRFGNSHLQIPPLLKPEPQRPAGRFTVCSADADADAGLPTLDTSAYLLNHHQQNS 180

Query: 181 GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           GSGSEITGPG       LIGSDFDTFSSFPTLESWKAVM
Sbjct: 181 GSGSEITGPG-------LIGSDFDTFSSFPTLESWKAVM 212

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BLAST of Carg01287 vs. ExPASy Swiss-Prot
Match: O80683 (Calmodulin-binding protein 25 OS=Arabidopsis thaliana OX=3702 GN=CAMBP25 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.7e-27
Identity = 98/242 (40.50%), Postives = 132/242 (54.55%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQ-DPW-ISDVFSRETDALSNPIHTS----HS-DLFSSFFD 60
           M +S+ L ++ D W +R  F  D W +SD FS + D L+  +HT+    H+    S+FFD
Sbjct: 1   MVTSEGLASV-DSWLYRQGFNVDSWLLSDTFSHDNDLLARALHTTVTAPHTLTPSSAFFD 60

Query: 61  LIA---PDSTPTPTPTGSGISADPEI--PAPNRPISSIPPTGRKITKRKSRASKRNHTTF 120
             A   P ST T + T SG S DPEI      R  + +   G K  KR++RASK++ TTF
Sbjct: 61  SSAVSHPSSTNTLSSTVSGAS-DPEIIGGGAKRKRNCLLTDG-KAAKRRARASKKSQTTF 120

Query: 121 ITADPANFRHMVQQVTGVRF---GNSHLQIPPVSKPEPQSPAGRFTVCG---ADAGLPTL 180
           ITADP+NFR MVQQVTG ++    +S     P+ KPEP     +   CG       +P L
Sbjct: 121 ITADPSNFRQMVQQVTGAKYIDDSSSFGIFDPIVKPEPLRFVNKLP-CGPSDRSTAVPML 180

Query: 181 DTSAYLLNHHHQN-------SGSGSEITGPGFGLSNDALIGS---DFDTFSSFPTLESWK 215
           DTSA+L NHH +N       SG+ S +  P    S  A  G    +FD + +FPTLESWK
Sbjct: 181 DTSAFLSNHHQENLAVGNAFSGNSSSVGLPSGKPSATADPGGSAVEFDNYPTFPTLESWK 238

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BLAST of Carg01287 vs. ExPASy TrEMBL
Match: A0A6J1GXM6 (calmodulin-binding protein 25-like OS=Cucurbita moschata OX=3662 GN=LOC111458101 PE=4 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 5.2e-111
Identity = 207/219 (94.52%), Postives = 208/219 (94.98%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60
           MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPI   HSDLFSSFFDLIAPDST
Sbjct: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPI---HSDLFSSFFDLIAPDST 60

Query: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120
           PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV
Sbjct: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120

Query: 121 QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVC----GADAGLPTLDTSAYLLNHHHQNS 180
           QQVTGVRFGNSHLQIPP+ KPEPQ PAGRFTVC     ADAGLPTLDTSAYLLNHH QNS
Sbjct: 121 QQVTGVRFGNSHLQIPPLLKPEPQRPAGRFTVCDADADADAGLPTLDTSAYLLNHHQQNS 180

Query: 181 GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM
Sbjct: 181 GSGSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216

[Close]

BLAST of Carg01287 vs. ExPASy TrEMBL
Match: A0A6J1I7A2 (calmodulin-binding protein 25-like OS=Cucurbita maxima OX=3661 GN=LOC111470257 PE=4 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 6.0e-107
Identity = 199/217 (91.71%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDST 60
           MASSDNLTTILDPWAFRPSFQDPWISDVFSRE  ALSNPIHTSHSD+ SSFFDLIAPDST
Sbjct: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSREAHALSNPIHTSHSDVLSSFFDLIAPDST 60

Query: 61  PTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120
           PTPTPT SGISAD     PNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV
Sbjct: 61  PTPTPTASGISAD-----PNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMV 120

Query: 121 QQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVCG--ADAGLPTLDTSAYLLNHHHQNSGS 180
           QQVTGVRFG+SHLQIPP+ KPEPQ PAGRFTVCG  ADAGLPTLDTSAYLLNHH QNSGS
Sbjct: 121 QQVTGVRFGSSHLQIPPLLKPEPQRPAGRFTVCGADADAGLPTLDTSAYLLNHHQQNSGS 180

Query: 181 GSEITGPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           GSEITGPGFGL+NDALIGSDFDTFSSFPTLESWKAVM
Sbjct: 181 GSEITGPGFGLTNDALIGSDFDTFSSFPTLESWKAVM 212

[Close]

BLAST of Carg01287 vs. ExPASy TrEMBL
Match: A0A6J1F6P7 (calmodulin-binding protein 25-like OS=Cucurbita moschata OX=3662 GN=LOC111441371 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.1e-71
Identity = 158/238 (66.39%), Postives = 173/238 (72.69%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTS----HSDLFSSFFDLIA 60
           MASSDN  T+LDPW FR +FQDPW SD FSRET  L+  +HTS     S  FSSF  LI+
Sbjct: 1   MASSDNFPTVLDPWPFRSTFQDPWNSDPFSRETQTLAKALHTSLLPTDSQSFSSFLHLIS 60

Query: 61  PDSTPTPTPTGSGISADPEIPAPNR----PISSIPPTGRKITKRKSRASKRNHTTFITAD 120
           P+S   PTPT S  S DP+  APNR      SSIPPT  KI+KRKSRASK+NHTTFITAD
Sbjct: 61  PESA-DPTPTVSAAS-DPDSAAPNRQTTTTTSSIPPTRGKISKRKSRASKKNHTTFITAD 120

Query: 121 PANFRHMVQQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVC---GADAGLPTLDTSAYLL 180
           PANFR MVQQVTGVRFGN+ LQIPPV KPEPQ P   F VC   GA AGLPTLDTSA+LL
Sbjct: 121 PANFRQMVQQVTGVRFGNTQLQIPPVLKPEPQRPTVSFAVCGGAGAGAGLPTLDTSAFLL 180

Query: 181 NHHH-----QNSGSGSEIT-------GPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           NHHH     Q+SGSGS+IT       GP  GL N+  IGSDFDTFSSFPTLESWKAV+
Sbjct: 181 NHHHHHHQQQSSGSGSDITGLGPLSFGPDLGLPNNDPIGSDFDTFSSFPTLESWKAVI 236

[Close]

BLAST of Carg01287 vs. ExPASy TrEMBL
Match: A0A6J1J8X4 (calmodulin-binding protein 25-like OS=Cucurbita maxima OX=3661 GN=LOC111482350 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 3.4e-70
Identity = 156/239 (65.27%), Postives = 172/239 (71.97%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHT----SHSDLFSSFFDLIA 60
           MASSDN  T+LDPW FR +FQDPW SD FSRET  L+  +HT    S S  FSSF  LI+
Sbjct: 1   MASSDNFPTVLDPWPFRSTFQDPWNSDPFSRETQTLAKALHTSLLPSDSQSFSSFLHLIS 60

Query: 61  PDSTPTPTPTGSGISADPEIPAPNRP--------ISSIPPTGRKITKRKSRASKRNHTTF 120
           P+S   PTPT S  S DP+  AP R          SSIPPT  KI+KRKSRASK+NHTTF
Sbjct: 61  PESA-DPTPTVSAAS-DPDSAAPKRQTTTTTTTMTSSIPPTRGKISKRKSRASKKNHTTF 120

Query: 121 ITADPANFRHMVQQVTGVRFGNSHLQIPPVSKPEPQSPAGRFTVC-GADAGLPTLDTSAY 180
           ITADPANFR MVQQVTGVRFGN+ LQIPPV KPEPQ P   F VC GA AGLPTLDTSA+
Sbjct: 121 ITADPANFRQMVQQVTGVRFGNTQLQIPPVLKPEPQRPTVSFAVCGGASAGLPTLDTSAF 180

Query: 181 LLNHHH----QNSGSGSEIT-------GPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           LLNHHH    Q+SG+GS+IT       GP  GL N+  IGSDFDTFSSFPTLESWKAV+
Sbjct: 181 LLNHHHHHQQQSSGNGSDITGLGPLSFGPDLGLPNNDPIGSDFDTFSSFPTLESWKAVI 237

[Close]

BLAST of Carg01287 vs. ExPASy TrEMBL
Match: A0A6J1DER1 (calmodulin-binding protein 25 OS=Momordica charantia OX=3673 GN=LOC111019825 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 3.3e-65
Identity = 141/233 (60.52%), Postives = 167/233 (71.67%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQDPWISDVFSRETDALSNPIHTS----HSDLFSSFFDLIA 60
           MASSDNL ++++PWAFR SF DPWISD FSR++DAL+  +H S     S + +SF DLI 
Sbjct: 1   MASSDNLASVVNPWAFRSSFLDPWISDPFSRDSDALTTALHRSLAGADSQVITSFLDLIK 60

Query: 61  PDSTPTPTPTGSGISA-----DPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITA 120
            D+ P  TP+ SG+SA     + E   P R   S+PP+G K++KRKSRASKRNHTTFITA
Sbjct: 61  SDAAPPSTPSVSGLSAGGAASEAETAPPRRQRGSVPPSGGKVSKRKSRASKRNHTTFITA 120

Query: 121 DPANFRHMVQQVTGVRFGN--SHLQIPPVSKPEPQSPAGRFTVCGADAGLPTLDTSAYLL 180
           D ANFR MVQQVTGVRFG+  S  Q  P+ KPEPQ P GR    GA   LPTLDTSA+LL
Sbjct: 121 DAANFRQMVQQVTGVRFGSSQSQAQTAPILKPEPQRPGGRLPGAGA---LPTLDTSAFLL 180

Query: 181 NHHHQNSGSGSEIT-------GPGFGLSNDALIGSDFDTFSSFPTLESWKAVM 216
           +HH Q+SGSGSEIT       GPG G++N   IGS FDTFS+FPTLESWKAVM
Sbjct: 181 SHHQQSSGSGSEITGPGPLDFGPGLGIANGGPIGSGFDTFSTFPTLESWKAVM 230

[Close]

BLAST of Carg01287 vs. TAIR 10
Match: AT3G56880.1 (VQ motif-containing protein )

HSP 1 Score: 135.2 bits (339), Expect = 6.3e-32
Identity = 102/251 (40.64%), Postives = 135/251 (53.78%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQ-DPW-ISDVFSRETDALSNPIHTSHSDLF-------SSF 60
           MASS+ L ++ DPW+FR +F  D W +SD FS ++D L+  +H S S          SSF
Sbjct: 1   MASSEGLASV-DPWSFRQNFNIDSWLLSDSFSHDSDILAKALHRSISTSTESSPLSPSSF 60

Query: 61  FD--LIAPDSTPTPTPTGSGISADPEIPAPN-----RPISSIPPTGRKITKRKSRAS-KR 120
           FD    A D +P  T +     +DPEIPA +     +       +G K TKR+SR S K+
Sbjct: 61  FDSSTAAVDFSPPQTLSNVSFGSDPEIPAASALGLGKRKRGPGVSGGKQTKRRSRVSNKK 120

Query: 121 NHTTFITADPANFRHMVQQVTGVRF-GNSHLQIPPVSKPEPQSPAGRF-TVCG---ADAG 180
           + TTFITAD ANFR MVQQVTG +F G+S+    P+ KPEP   A R    CG     + 
Sbjct: 121 SQTTFITADAANFRQMVQQVTGAKFLGSSNSIFAPIVKPEPHRLASRLPPSCGNLDRSSA 180

Query: 181 LPTLDTSAYLLNHH---------------HQNSGSGSEITGPGFGLSNDALIGSDFDTFS 215
           +PTLDTS++L NHH               H  S +G+     G G S       + D++ 
Sbjct: 181 VPTLDTSSFLSNHHQENIITDLGAPTGSFHHQSSAGTTTANVGGGSS-----AVELDSYP 240

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BLAST of Carg01287 vs. TAIR 10
Match: AT2G41010.1 (calmodulin (CAM)-binding protein of 25 kDa )

HSP 1 Score: 123.6 bits (309), Expect = 1.9e-28
Identity = 98/242 (40.50%), Postives = 132/242 (54.55%), Query Frame = 0


Query: 1   MASSDNLTTILDPWAFRPSFQ-DPW-ISDVFSRETDALSNPIHTS----HS-DLFSSFFD 60
           M +S+ L ++ D W +R  F  D W +SD FS + D L+  +HT+    H+    S+FFD
Sbjct: 1   MVTSEGLASV-DSWLYRQGFNVDSWLLSDTFSHDNDLLARALHTTVTAPHTLTPSSAFFD 60

Query: 61  LIA---PDSTPTPTPTGSGISADPEI--PAPNRPISSIPPTGRKITKRKSRASKRNHTTF 120
             A   P ST T + T SG S DPEI      R  + +   G K  KR++RASK++ TTF
Sbjct: 61  SSAVSHPSSTNTLSSTVSGAS-DPEIIGGGAKRKRNCLLTDG-KAAKRRARASKKSQTTF 120

Query: 121 ITADPANFRHMVQQVTGVRF---GNSHLQIPPVSKPEPQSPAGRFTVCG---ADAGLPTL 180
           ITADP+NFR MVQQVTG ++    +S     P+ KPEP     +   CG       +P L
Sbjct: 121 ITADPSNFRQMVQQVTGAKYIDDSSSFGIFDPIVKPEPLRFVNKLP-CGPSDRSTAVPML 180

Query: 181 DTSAYLLNHHHQN-------SGSGSEITGPGFGLSNDALIGS---DFDTFSSFPTLESWK 215
           DTSA+L NHH +N       SG+ S +  P    S  A  G    +FD + +FPTLESWK
Sbjct: 181 DTSAFLSNHHQENLAVGNAFSGNSSSVGLPSGKPSATADPGGSAVEFDNYPTFPTLESWK 238

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BLAST of Carg01287 vs. TAIR 10
Match: AT4G39720.1 (VQ motif-containing protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.7e-05
Identity = 25/47 (53.19%), Postives = 33/47 (70.21%), Query Frame = 0


Query: 84  SSIPPTGR----KITKRKSRASKRNHTTFITADPANFRHMVQQVTGV 127
           SS+PPT      K TK++SRAS+R  TT +T D +NFR MVQ+ TG+
Sbjct: 100 SSLPPTNNIGVIKKTKKRSRASRRAPTTVLTTDTSNFRAMVQEFTGI 146

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BLAST of Carg01287 vs. TAIR 10
Match: AT4G15120.1 (VQ motif-containing protein )

HSP 1 Score: 46.2 bits (108), Expect = 3.8e-05
Identity = 35/77 (45.45%), Postives = 43/77 (55.84%), Query Frame = 0


Query: 59  STPTPTPTGSGISADPEIPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRH 118
           ST T T T +  SAD  +   NR ++       K T+R+SRAS+R  TT    D ANFR 
Sbjct: 23  STVTTTTT-TATSADSPLSPDNRRVA-------KPTRRRSRASRRTPTTLFNTDTANFRA 82

Query: 119 MVQQVTG----VRFGNS 132
           MVQQ TG    V FG+S
Sbjct: 83  MVQQFTGGPSAVAFGSS 91

[Close]

BLAST of Carg01287 vs. TAIR 10
Match: AT1G35830.1 (VQ motif-containing protein )

HSP 1 Score: 45.1 bits (105), Expect = 8.5e-05
Identity = 36/111 (32.43%), Postives = 55/111 (49.55%), Query Frame = 0


Query: 16  FRPSFQDPWISDVFSRETDALSNPIHTSHSDLFSSFFDLIAPDSTPTPTPTGSGISADPE 75
           F P FQ P  S  +  +   LS+   +S S  + +F + +  D     T         P+
Sbjct: 31  FLPPFQPPPSSSTYYGDPTTLSS--SSSSSATYLNFVNNLISDDILNQTHL-----LPPQ 90

Query: 76  IPAPNRPISSIPPTGRKITKRKSRASKRNHTTFITADPANFRHMVQQVTGV 127
            P P  P    PP+  +  ++++RAS+R  TT +T D +NFR MVQ+ TGV
Sbjct: 91  PPPPPPP---PPPSSSRNPRKRTRASRRAPTTVLTTDTSNFRAMVQEFTGV 131

The following BLAST results are available for this feature:

BLAST of Carg01287 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027718.1	2.2e-119	100.00	Calmodulin-binding protein 25, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
KAG6596181.1	1.0e-116	97.72	Calmodulin-binding protein 25, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022956380.1	1.1e-110	94.52	calmodulin-binding protein 25-like [Cucurbita moschata]	[more]
XP_022971583.1	1.2e-106	91.71	calmodulin-binding protein 25-like [Cucurbita maxima]	[more]
XP_023539162.1	4.7e-106	90.87	calmodulin-binding protein 25-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_0...	[more]

BLAST of Carg01287 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80683	2.7e-27	40.50	Calmodulin-binding protein 25 OS=Arabidopsis thaliana OX=3702 GN=CAMBP25 PE=1 SV...	[more]

BLAST of Carg01287 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GXM6	5.2e-111	94.52	calmodulin-binding protein 25-like OS=Cucurbita moschata OX=3662 GN=LOC111458101...	[more]
A0A6J1I7A2	6.0e-107	91.71	calmodulin-binding protein 25-like OS=Cucurbita maxima OX=3661 GN=LOC111470257 P...	[more]
A0A6J1F6P7	3.1e-71	66.39	calmodulin-binding protein 25-like OS=Cucurbita moschata OX=3662 GN=LOC111441371...	[more]
A0A6J1J8X4	3.4e-70	65.27	calmodulin-binding protein 25-like OS=Cucurbita maxima OX=3661 GN=LOC111482350 P...	[more]
A0A6J1DER1	3.3e-65	60.52	calmodulin-binding protein 25 OS=Momordica charantia OX=3673 GN=LOC111019825 PE=...	[more]

BLAST of Carg01287 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56880.1	6.3e-32	40.64	VQ motif-containing protein	[more]
AT2G41010.1	1.9e-28	40.50	calmodulin (CAM)-binding protein of 25 kDa	[more]
AT4G39720.1	1.7e-05	53.19	VQ motif-containing protein	[more]
AT4G15120.1	3.8e-05	45.45	VQ motif-containing protein	[more]
AT1G35830.1	8.5e-05	32.43	VQ motif-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008889	VQ	PFAM	PF05678	VQ	coord: 105..125 e-value: 2.2E-8 score: 33.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..102
None	No IPR available	PANTHER	PTHR33179:SF9	CALMODULIN-BINDING PROTEIN 25	coord: 4..213
IPR039609	VQ motif-containing protein 15/22	PANTHER	PTHR33179	VQ MOTIF-CONTAINING PROTEIN	coord: 4..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01287-RA	Carg01287-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006970	response to osmotic stress
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005516	calmodulin binding