Carg01271 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01271
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	NADH:cytochrome B5 reductase 1
Location	Carg_Chr06: 295623 .. 299231 (+)
RNA-Seq Expression	Carg01271
Synteny	Carg01271

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTACTTGACCAGGACTCAAATCCAACTCCTTAAACCCACCATTTTTCTTCCTTCTTACCTACCCCTCATTCTTCCAACTCCCCCAATTTCTGATGGAGATCTTACAGGCTCTCGATGCTCAGGTTCTCGTTGGCTTAGCCGCCGCCCTGGTTGCCATTGTTGCTGGTGCCTTTTTCCTTGTCTCCTCCAGAAAACCAGCATCCACAGGTTTTACTCCACCTTCTGATTTGATTCTTTGAAATGGAAGCCTTATGGGTCTTTATTTACTGCTTTCCAATTCACTATCCAGTTTGAATTTGTGATGCTCTGATGTTTTGATTCGTGGGTTGTGATGTTTTTGAACTGTGTTGAAGTATTTATATAAGATTCAATTTATACTGATCCATTAGCTTAGCCTTTTACTTTTTATGATGATTTTACATTGCTACAAGGGAGGAAGTTATCTCCTCCTTGATTAATGTTGATACCTACATGTTTTGATTACCACCTCTAGTTGATATTGTCTGCTTTTGCTCGTTACGTATCGCCGTCAGCCTCACGATTTTTAAAACGTATCTATTAGGGAGAGGTTTCCACACCCTTGTAAGGAATGCTTTGTTCCCTTCTTAAACCGATGCAGGATCTCACAATTCTTGGGGGCCAAACGTCCTTGCTAGTACACTACCCAGTGTCTAGCTCTACCATTTGTAACAGCCCAAACTCACCGCTAGTAGATATCGTCCGCTTTGGCCCATTACATATTGCCATCAGCCTCACGGTTTTCAAACGCGGTTGCTAGGGAGAGGTTTCCACACCCTTATAAGGAATGCTTTGTTCCCCTCTTCAACCAATGCAGGATCTCACAATCCTTGGTGGCCCAACGTCTTCACTGGCACACCGCCTAGTTCTAGCTCTACCATTTGTAACAGCCTAAGCCCACCGCTGATTGATCGAAATTCCAATGGCCATGAAAATTATCTTCATCTTTGTTGAGCTCTTGGTCAATGTGAAATGGTAGATGTTATGGGAAATATGCATTTTTAAAAAGATAATGCTCTTTTCTTGCCCTGTCTCTTATGAGAGGATTAACACCTATAATGTTATCTAATTAGCAGCTAATGTTCTTGAACCATCTTTTCTTGATATCTATCACAATTTCTTGATATCTATCACAATTTCTTGATATCTTAATAAAACTGATTGGCTCATATGTGGCATGTGGTTTGTGACTACAGCCTGCTTGGATCCTGAGAACTTTAAGGAGTTTAAGCTTGTTAAGCGCACGAAGCTGAGCCATAATGTGGCAAAGTTCACTTTTATACTTCCCAAGACCGATTCAATTCTTGGTCTTCCTATTGGACAGCATCTAAGTTGCAGGTTTCATTTTATCCATTATAGCGATACAGCTATGATACTCAGCAGTTAACACGCCTAATATTTGGTTCATTTTCCCTCTCTGTTTAAAAACATATGTGCAGGGGAAAGGATAGCCAAGGTGAAGAGGTTATTAAATCTTACACTCCAGTTACCTTGGACTCTGATGTTGGATATTTTGAGCTAGTCATAAAGGTACATGTATAAATCTGCTACAGGTTAATGATGTCACATTGATTATCGTTATTCCACTTCTGATTCTTATATGATTGTGTGTGTGATTTAGATGTATCCCCAAGGAAGGATGTCGCATCATTTTCGAGAAATGCGTATGGGGGACTTTCTTGCTGTGAAGGGACCAAAGGTATGTCTTCCTTGTACACAAACTTTGACAACAGTTTTGGAATGTGTTATTGTTTGATTTTGTATGAAGCTTTGGTAGCGTAGCAGATATTGTCCTCTTTGGGCTTTCCCTTTTCGAGCTTCCCCTCAAGGTTTTTGAAACGCGTCTGCTATGAAAAGGTTTTCACACCCTTATAAAGAATGCTTCGTTTTCCTCCCCAACCGATGTGGGATCTCACAATCCACTCTCCTTCAGAGCCAGCATCCTCACTGGCACTCGTTCCTTTCTCCAATCAATGTAGGACCCCCAAATCCACCCCCTTCTGGGCTCAGCCTCCTCCATGGCATATCGCCCGGTGTTTGGCTCATATACCATTTGTAACGGTCCAAGCCCACTGCTGGTAGATGTTGTCCTCTTTGGACTTTCTCTTTCAGGCTTCCTCTCAAGGTTTTTAAGACGCGTCAACTAGGGAGAAGTTTTCACACCCTTATACAGAATGCTTCGTTCTTCCAAGTCCCACTAAAATGATCTACCACAAAACTAAGCTCCCTAAAACTTCTATTTGTTTAACCAAGACACCTCTAACTAATAACAACTATGTCCTTATTAATAACATACCAATCCCCCACGTAGGATCTATGTCCTTATAGAATTGAGTTGCTTTGAATGCGGCCTATTTTAGAAACTTATGTACTCCATCAATTATTGCAGGGAAGTTTTAAGTATCAACCTGGTAAAGTTAGAGCATTTGGGATGCTCGCTGGAGGCTCTGGCATTACACCCATGTATCAAGTAATGAAACAAACTTTTCTTCTACAACACATTTTCCCTTATTCTTGGAATCTTGGCTCTATTTTGTTGTTTCATTGTCTCTTGTCATGTTTTAAAACAAGACAAAACTCTCTCATTTTTACTTCTGTTCTAGGTTGCTCGGTCTATATTAGAAAACCTGAACGACAAGACACAGATTCACCTTATTTATGCCAATGTTACACTTGAAGATATTCTTTTGAAGGTAATTATTTTCTTTGCTTTTTTCTTCAACTTCTTACTCTCTTCCCATTAGAAATACATAACAATACAATAATCACTTGTATTTGCAGGAGGAATTGGATCTTCTTGCCAAGAGATACCCTGATCGCTTCCAGATCTACTATGTCTTGAACCAGGTCAGTTAAAAGTTTAAACTTAGTATTAATCTAATACAATGGTACGAGGATACTAAGAATATATTAAATAACGACTTTAGAGTATTTGTGTAATGTTTAATGTTACTACTAATGTTCTTTGTACATTGAAATAGCCTCCTAAAGTTTGGGATGGCGGCGTCGGGTTTGTGTCGAAGGAAATGATTCGAGCGCATTGCCCTGCACCTGCCTCTGACATTCAGGTACTAGGTTTAAATCTTATTTTGGTCTCTAAACTTTCATGTTTATATGTTTTAGTTCCTTCGAAATGTGCTCCTAAACCTGTTAAAAACCGTTATAGTCATTATAGTTACTTTTGATAAGTTATTTATGTAAAGTTTATGTGATATACGATAAGATTCGATAATTTAGGGACCATGATAGGATTTGAACTTCGAAACGTTCTTCATAGCACGACATCTATGATAGCTTCTAACTTTTTTTTCGAACCAATGTGTTTAGATTTTGCGGTGTGGACCGCCGCCGATGAACAAGGCCATGGCTGCCCACCTTGATGCTCTTGGATATACACCTGAAATGCAGTTCCAGTTCTAGTTCATGGTGACTCTTCAATATTTGGTGATTTAAAGATGAGGACTTCAACTTTCATTCTAAAAATTTAGTTGTATGAGTGAGCATCAAAGATGTGAACTATTATGTGTAACTTTTAAAACATGGCTCTGTTTTCGCGTTACAATTGATGTCAATAAAATGGGAATAATTT

mRNA sequence

ATTTACTTGACCAGGACTCAAATCCAACTCCTTAAACCCACCATTTTTCTTCCTTCTTACCTACCCCTCATTCTTCCAACTCCCCCAATTTCTGATGGAGATCTTACAGGCTCTCGATGCTCAGGTTCTCGTTGGCTTAGCCGCCGCCCTGGTTGCCATTGTTGCTGGTGCCTTTTTCCTTGTCTCCTCCAGAAAACCAGCATCCACAGCCTGCTTGGATCCTGAGAACTTTAAGGAGTTTAAGCTTGTTAAGCGCACGAAGCTGAGCCATAATGTGGCAAAGTTCACTTTTATACTTCCCAAGACCGATTCAATTCTTGGTCTTCCTATTGGACAGCATCTAAGTTGCAGGGGAAAGGATAGCCAAGGTGAAGAGGTTATTAAATCTTACACTCCAGTTACCTTGGACTCTGATGTTGGATATTTTGAGCTAGTCATAAAGATGTATCCCCAAGGAAGGATGTCGCATCATTTTCGAGAAATGCGTATGGGGGACTTTCTTGCTGTGAAGGGACCAAAGGGAAGTTTTAAGTATCAACCTGGTAAAGTTAGAGCATTTGGGATGCTCGCTGGAGGCTCTGGCATTACACCCATGTATCAAGTTGCTCGGTCTATATTAGAAAACCTGAACGACAAGACACAGATTCACCTTATTTATGCCAATGTTACACTTGAAGATATTCTTTTGAAGGAGGAATTGGATCTTCTTGCCAAGAGATACCCTGATCGCTTCCAGATCTACTATGTCTTGAACCAGCCTCCTAAAGTTTGGGATGGCGGCGTCGGGTTTGTGTCGAAGGAAATGATTCGAGCGCATTGCCCTGCACCTGCCTCTGACATTCAGATTTTGCGGTGTGGACCGCCGCCGATGAACAAGGCCATGGCTGCCCACCTTGATGCTCTTGGATATACACCTGAAATGCAGTTCCAGTTCTAGTTCATGGTGACTCTTCAATATTTGGTGATTTAAAGATGAGGACTTCAACTTTCATTCTAAAAATTTAGTTGTATGAGTGAGCATCAAAGATGTGAACTATTATGTGTAACTTTTAAAACATGGCTCTGTTTTCGCGTTACAATTGATGTCAATAAAATGGGAATAATTT

Coding sequence (CDS)

ATGGAGATCTTACAGGCTCTCGATGCTCAGGTTCTCGTTGGCTTAGCCGCCGCCCTGGTTGCCATTGTTGCTGGTGCCTTTTTCCTTGTCTCCTCCAGAAAACCAGCATCCACAGCCTGCTTGGATCCTGAGAACTTTAAGGAGTTTAAGCTTGTTAAGCGCACGAAGCTGAGCCATAATGTGGCAAAGTTCACTTTTATACTTCCCAAGACCGATTCAATTCTTGGTCTTCCTATTGGACAGCATCTAAGTTGCAGGGGAAAGGATAGCCAAGGTGAAGAGGTTATTAAATCTTACACTCCAGTTACCTTGGACTCTGATGTTGGATATTTTGAGCTAGTCATAAAGATGTATCCCCAAGGAAGGATGTCGCATCATTTTCGAGAAATGCGTATGGGGGACTTTCTTGCTGTGAAGGGACCAAAGGGAAGTTTTAAGTATCAACCTGGTAAAGTTAGAGCATTTGGGATGCTCGCTGGAGGCTCTGGCATTACACCCATGTATCAAGTTGCTCGGTCTATATTAGAAAACCTGAACGACAAGACACAGATTCACCTTATTTATGCCAATGTTACACTTGAAGATATTCTTTTGAAGGAGGAATTGGATCTTCTTGCCAAGAGATACCCTGATCGCTTCCAGATCTACTATGTCTTGAACCAGCCTCCTAAAGTTTGGGATGGCGGCGTCGGGTTTGTGTCGAAGGAAATGATTCGAGCGCATTGCCCTGCACCTGCCTCTGACATTCAGATTTTGCGGTGTGGACCGCCGCCGATGAACAAGGCCATGGCTGCCCACCTTGATGCTCTTGGATATACACCTGAAATGCAGTTCCAGTTCTAG

Protein sequence

MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHNVAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLNDKTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF

Homology

[Close]

BLAST of Carg01271 vs. NCBI nr
Match: KAG7027734.1 (NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 562.4 bits (1448), Expect = 2.2e-156
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of Carg01271 vs. NCBI nr
Match: KAG6596195.1 (NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560.8 bits (1444), Expect = 6.5e-156
Identity = 279/280 (99.64%), Postives = 279/280 (99.64%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           MEILQA DAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN
Sbjct: 1   MEILQAFDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of Carg01271 vs. NCBI nr
Match: XP_022939629.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita moschata])

HSP 1 Score: 559.7 bits (1441), Expect = 1.4e-155
Identity = 278/280 (99.29%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           MEILQALDAQVLVGLAAA+VAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLVGLAAAVVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPG+VRAFGMLAGGSGITPMYQVARSILENLND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGQVRAFGMLAGGSGITPMYQVARSILENLND 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of Carg01271 vs. NCBI nr
Match: XP_023540168.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.7 bits (1410), Expect = 5.7e-152
Identity = 272/280 (97.14%), Postives = 276/280 (98.57%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           MEILQALDAQVL+GLA ALVAIVAGAFFLVSSRK AS ACLDPENFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLIGLATALVAIVAGAFFLVSSRKSASRACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPG+VRAFGMLAGGSGITPMYQVARSILEN ND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGQVRAFGMLAGGSGITPMYQVARSILENPND 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT+IHLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of Carg01271 vs. NCBI nr
Match: XP_022971225.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita maxima])

HSP 1 Score: 545.8 bits (1405), Expect = 2.2e-151
Identity = 272/280 (97.14%), Postives = 275/280 (98.21%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           MEILQALDAQVL GLAAALVAIVAGAFFLVSSRKPAS ACLDP+NFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILEN ND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT+IHLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPAS IQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of Carg01271 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.2e-123
Identity = 217/280 (77.50%), Postives = 246/280 (87.86%), Query Frame = 0


Query: 2   EILQALDAQVLVGLAAALVAIVAG-AFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 61
           E L+ LD Q+L+G+  A VA+ AG A+FL SS+K     CLDPENFKEFKLVKR +LSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKK--RRVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 121
           VAKF F LP + S+LGLPIGQH+SCRGKD QGE+VIK YTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 181
           GRMSHHFREMR+GD LAVKGPKG FKYQPG+ RAFGMLAGGSGITPM+QVAR+ILEN  D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 241
           KT++HLIYANVT +DILLKEEL+ L   YP++F+I+YVLNQPP+VWDGGVGFVSKEMI+ 
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPASDIQILRCGPPPMNKAMAA+L+ALGY+PEMQFQF
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of Carg01271 vs. ExPASy Swiss-Prot
Match: A1DHW1 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-70
Identity = 135/267 (50.56%), Postives = 181/267 (67.79%), Query Frame = 0


Query: 13  VGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHNVAKFTFILPKTD 72
           V  A AL AI+AG        KP     L+P  F+EF L ++T +SHNV  + F LP+  
Sbjct: 31  VPYAVALTAILAGLKLFTGGSKPRKV--LNPTEFQEFVLKEKTDISHNVCIYRFALPRPA 90

Query: 73  SILGLPIGQHLSCRGK-DSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREMR 132
            ILGLPIGQH+S     + Q +EV++SYTP++ D++ GYF+L++K YPQG +S +   ++
Sbjct: 91  DILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLK 150

Query: 133 MGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENL-----NDKTQIHL 192
           +GD + V+GPKG+  Y P   R  GM+AGG+GITPM Q+ ++++ N      ND T++ L
Sbjct: 151 IGDTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDL 210

Query: 193 IYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPA 252
           I+ANV  +DILLKEELD LA   PD F IYYVLN PP+ W GGVGFV+ EMI+ H PAPA
Sbjct: 211 IFANVNPDDILLKEELDKLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPA 270

Query: 253 SDIQILRCGPPPMNKAMAAHLDALGYT 274
           SD++IL CGPPPM  AM    ++LGYT
Sbjct: 271 SDVKILLCGPPPMISAMKKATESLGYT 294

[Close]

BLAST of Carg01271 vs. ExPASy Swiss-Prot
Match: Q4X0B5 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 266.5 bits (680), Expect = 3.3e-70
Identity = 133/267 (49.81%), Postives = 181/267 (67.79%), Query Frame = 0


Query: 13  VGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHNVAKFTFILPKTD 72
           V  A A+ AI+AG        KP     L+P  F+EF L ++  +SHNV  + F LP+  
Sbjct: 31  VPYAVAVTAILAGLKLFTGGNKPRKV--LNPTEFQEFVLKEKIDVSHNVCIYRFALPRPT 90

Query: 73  SILGLPIGQHLSCRGK-DSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREMR 132
            ILGLPIGQH+S     + Q +EV++SYTP++ D++ GYF+L++K YPQG +S +   ++
Sbjct: 91  DILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLK 150

Query: 133 MGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENL-----NDKTQIHL 192
           +GD + V+GPKG+  Y P   R  GM+AGG+GITPM Q+ ++++ N      ND T++ L
Sbjct: 151 IGDTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDL 210

Query: 193 IYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPA 252
           I+ANV  +DILLKEELD+LA   PD F IYYVLN PP+ W GGVGFV+ EMI+ H PAPA
Sbjct: 211 IFANVNPDDILLKEELDMLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPA 270

Query: 253 SDIQILRCGPPPMNKAMAAHLDALGYT 274
           SD++IL CGPPPM  AM    ++LGYT
Sbjct: 271 SDVKILLCGPPPMISAMKKATESLGYT 294

[Close]

BLAST of Carg01271 vs. ExPASy Swiss-Prot
Match: Q54NC1 (NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 4.8e-69
Identity = 123/240 (51.25%), Postives = 172/240 (71.67%), Query Frame = 0


Query: 41  LDPENFKEFKLVKRTKLSHNVAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYT 100
           LDP+ +++F+L ++  ++HN   F F LP  D ILGLPIGQH+S R     G+EV + YT
Sbjct: 49  LDPQEYRKFQLKEKFIVNHNTRIFRFALPNEDDILGLPIGQHISLRAVVG-GKEVYRPYT 108

Query: 101 PVTLDSDVGYFELVIKMYPQGRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAG 160
           P++ D + GYF+L+IK+Y +G MS +   M +GD + VKGPKG F YQP   ++ GMLAG
Sbjct: 109 PISSDEERGYFDLLIKVYEKGAMSGYVDNMFIGDSIEVKGPKGKFNYQPNMRKSIGMLAG 168

Query: 161 GSGITPMYQVARSILENLNDKTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLN 220
           G+GITPM QV ++IL+N +DKT+I L++ N+T EDILLK+ELD LA+++P +F++YYVLN
Sbjct: 169 GTGITPMLQVIKAILKNPSDKTEISLVFGNITEEDILLKKELDELAEKHP-QFKVYYVLN 228

Query: 221 QPPKVWDGGVGFVSKEMIRAHCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280
            PPK W  GVGFVSKE+I +  P+P+    ++ CGPP MNKAM  HL+ +G+     F F
Sbjct: 229 NPPKGWTQGVGFVSKEIIESRLPSPSDQTMVIMCGPPMMNKAMTGHLETIGFNESNIFTF 286

[Close]

BLAST of Carg01271 vs. ExPASy Swiss-Prot
Match: Q2UFN3 (NADH-cytochrome b5 reductase 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 261.9 bits (668), Expect = 8.1e-69
Identity = 131/268 (48.88%), Postives = 183/268 (68.28%), Query Frame = 0


Query: 12  LVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHNVAKFTFILPKT 71
           L+ +  A+ A++ GA FL  S KP     L+P  F+ F L ++ ++SHNVA + F LP+ 
Sbjct: 13  LLVVGTAIFAVLVGAKFLGGSGKPRKV--LNPTEFQNFVLKEKNEISHNVAIYRFALPRP 72

Query: 72  DSILGLPIGQHLSCRGK-DSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREM 131
             ILGLPIGQH+S     + Q +EV++SYTP++ D++ GYF+L++K YPQG +S +   +
Sbjct: 73  TDILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTL 132

Query: 132 RMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENL-----NDKTQIH 191
           ++GD + V+GPKG+  Y P   R  GM+AGG+GITPM Q+ ++I+ N      ND TQ+ 
Sbjct: 133 KIGDNMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAIIRNRPRNGGNDTTQVD 192

Query: 192 LIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAP 251
           LI+ANV  EDILLKEEL+ L K   D F++YYVLN PP+ W GGVGFV+ +MI+   PAP
Sbjct: 193 LIFANVNPEDILLKEELEQLVKE-DDGFRVYYVLNNPPEGWTGGVGFVTPDMIKERLPAP 252

Query: 252 ASDIQILRCGPPPMNKAMAAHLDALGYT 274
           A DI+I+ CGPPPM  AM    ++LGYT
Sbjct: 253 AQDIKIMLCGPPPMISAMKKATESLGYT 277

[Close]

BLAST of Carg01271 vs. ExPASy TrEMBL
Match: A0A6J1FHS1 (NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111445460 PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 7.0e-156
Identity = 278/280 (99.29%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           MEILQALDAQVLVGLAAA+VAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLVGLAAAVVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPG+VRAFGMLAGGSGITPMYQVARSILENLND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGQVRAFGMLAGGSGITPMYQVARSILENLND 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of Carg01271 vs. ExPASy TrEMBL
Match: A0A6J1I558 (NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111470015 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.0e-151
Identity = 272/280 (97.14%), Postives = 275/280 (98.21%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           MEILQALDAQVL GLAAALVAIVAGAFFLVSSRKPAS ACLDP+NFKEFKLVKRTKLSHN
Sbjct: 1   MEILQALDAQVLTGLAAALVAIVAGAFFLVSSRKPASRACLDPDNFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKF FILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFIFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILEN ND
Sbjct: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENPND 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT+IHLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPPKVWDGGVGFVSKEMIRA
Sbjct: 181 KTKIHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPKVWDGGVGFVSKEMIRA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPAS IQILRCGPPPMNKAMAAHLDALGYTPEMQFQF
Sbjct: 241 HCPAPASYIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 280

[Close]

BLAST of Carg01271 vs. ExPASy TrEMBL
Match: A0A1S3B6Z6 (NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103486847 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 6.8e-143
Identity = 252/280 (90.00%), Postives = 266/280 (95.00%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           ME+LQALD+QVL+GL AALVAIV GA FL SSRKPAS ACLDPE FKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTP TLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGD LAVKGPKG FKYQPG+V AFGMLAGGSGITPMYQV+R+ILEN  D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT++HLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPP+VWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPA+DI+ILRCGPPPMNKAM  HLDALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of Carg01271 vs. ExPASy TrEMBL
Match: A0A5D3DPB8 (NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006230 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 6.8e-143
Identity = 252/280 (90.00%), Postives = 266/280 (95.00%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           ME+LQALD+QVL+GL AALVAIV GA FL SSRKPAS ACLDPE FKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTP TLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGD LAVKGPKG FKYQPG+V AFGMLAGGSGITPMYQV+R+ILEN  D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT++HLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPP+VWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPA+DI+ILRCGPPPMNKAM  HLDALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of Carg01271 vs. ExPASy TrEMBL
Match: E5GBY3 (NADH-cytochrome b5 reductase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 6.8e-143
Identity = 252/280 (90.00%), Postives = 266/280 (95.00%), Query Frame = 0


Query: 1   MEILQALDAQVLVGLAAALVAIVAGAFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 60
           ME+LQALD+QVL+GL AALVAIV GA FL SSRKPAS ACLDPE FKEFKLVKRTKLSHN
Sbjct: 1   MEVLQALDSQVLIGLVAALVAIVVGAVFLFSSRKPASRACLDPEKFKEFKLVKRTKLSHN 60

Query: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 120
           VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTP TLDSDVGYFELVIKMYPQ
Sbjct: 61  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPTTLDSDVGYFELVIKMYPQ 120

Query: 121 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 180
           GRMSHHFREMRMGD LAVKGPKG FKYQPG+V AFGMLAGGSGITPMYQV+R+ILEN  D
Sbjct: 121 GRMSHHFREMRMGDSLAVKGPKGRFKYQPGQVSAFGMLAGGSGITPMYQVSRAILENPED 180

Query: 181 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 240
           KT++HLIYANVTLEDILLKEELDLLAKRYPDRF+IYYVLNQPP+VWDGGVGFVSKEMI+A
Sbjct: 181 KTKVHLIYANVTLEDILLKEELDLLAKRYPDRFKIYYVLNQPPEVWDGGVGFVSKEMIKA 240

Query: 241 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPA+DI+ILRCGPPPMNKAM  HLDALGY+PEMQFQF
Sbjct: 241 HCPAPAADIKILRCGPPPMNKAMGEHLDALGYSPEMQFQF 280

[Close]

BLAST of Carg01271 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 444.1 bits (1141), Expect = 8.2e-125
Identity = 217/280 (77.50%), Postives = 246/280 (87.86%), Query Frame = 0


Query: 2   EILQALDAQVLVGLAAALVAIVAG-AFFLVSSRKPASTACLDPENFKEFKLVKRTKLSHN 61
           E L+ LD Q+L+G+  A VA+ AG A+FL SS+K     CLDPENFKEFKLVKR +LSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSKK--RRVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKSYTPVTLDSDVGYFELVIKMYPQ 121
           VAKF F LP + S+LGLPIGQH+SCRGKD QGE+VIK YTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAFGMLAGGSGITPMYQVARSILENLND 181
           GRMSHHFREMR+GD LAVKGPKG FKYQPG+ RAFGMLAGGSGITPM+QVAR+ILEN  D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 KTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRA 241
           KT++HLIYANVT +DILLKEEL+ L   YP++F+I+YVLNQPP+VWDGGVGFVSKEMI+ 
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDIQILRCGPPPMNKAMAAHLDALGYTPEMQFQF 281
           HCPAPASDIQILRCGPPPMNKAMAA+L+ALGY+PEMQFQF
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of Carg01271 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 202.6 bits (514), Expect = 4.2e-52
Identity = 111/258 (43.02%), Postives = 156/258 (60.47%), Query Frame = 0


Query: 41  LDPENFKEFKLVKRTKLSHNVAKFTFILPKTDSILGLPIGQHLSCR---GKDSQGEE--V 100
           L+P+ + EFKL    ++SHN   F F    + + LGL +   L  R   G +++G+   V
Sbjct: 73  LNPDKWLEFKLQDTARVSHNTQLFRFSFDPS-AELGLHVASCLLTRAPLGYNAEGKTKYV 132

Query: 101 IKSYTPVTLDSDVGYFELVIKMYPQGRMSHHFREMRMGDFLAVKGPKGSFKYQPGKVRAF 160
           I+ YTP++     GYF+L+IK+YP G+MS HF  ++ GD L VKGP   FKY P   +  
Sbjct: 133 IRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGDVLEVKGPVEKFKYSPNMKKHI 192

Query: 161 GMLAGGSGITPMYQVARSILENLNDKTQIHLIYANVTLEDILLKEELDLLAKRYPDRFQI 220
           GM+AGGSGITPM QV  +I++N  D TQI L+YANV+ +DILLK++LD+L   +P+  +I
Sbjct: 193 GMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPDDILLKQKLDVLQANHPN-LKI 252

Query: 221 YYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASDIQILRCGPPPMNKAMAAH--------- 280
           +Y ++ P K W GGVG++SK+M     P P  D  IL CGPP M + ++           
Sbjct: 253 FYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVCGPPGMMEHISGGKAPDWSQGE 312

[Close]

BLAST of Carg01271 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 173.3 bits (438), Expect = 2.7e-43
Identity = 97/261 (37.16%), Postives = 155/261 (59.39%), Query Frame = 0


Query: 39  ACLDPENFKEFKLVKRTKLSHNVAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKS 98
           A ++P      +LV++T +SH+V KF F LP  D +LGLP+G+H+      +  +  +++
Sbjct: 655 ALVNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND-KLCLRA 714

Query: 99  YTPVTLDSDVGYFELVIKMY-----PQ----GRMSHHFREMRMGDFLAVKGPKGSFKY-- 158
           YTP +    VGYFELV+K+Y     P+    G MS +   + +G  L +KGP G  +Y  
Sbjct: 715 YTPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLG 774

Query: 159 --------QPGKVRAFGMLAGGSGITPMYQVARSILENLNDKTQIHLIYANVTLEDILLK 218
                   +P       MLAGG+GITP+YQ+ ++IL++  D+T++++IYAN T EDILL+
Sbjct: 775 KGSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLR 834

Query: 219 EELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASDIQI-LRCGPPP 278
           EELD  A++YPDR +++YV+    + W    GF+S+ ++R H P       + + CGPPP
Sbjct: 835 EELDGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPP 894

[Close]

BLAST of Carg01271 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 165.2 bits (417), Expect = 7.4e-41
Identity = 88/255 (34.51%), Postives = 150/255 (58.82%), Query Frame = 0


Query: 39  ACLDPENFKEFKLVKRTKLSHNVAKFTFILPKTDSILGLPIGQHLSCRGKDSQGEEVIKS 98
           A ++P      +L+++T +SH+V KF F LP  D  LGLP+G+H+     +   +  +++
Sbjct: 655 ALVNPREKIPVRLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCA-NINDKLCLRA 714

Query: 99  YTPVTLDSDVGYFELVIKMY-----PQ----GRMSHHFREMRMGDFLAVKGPKGSFKY-- 158
           YTP +    VG+ +LV+K+Y     P+    G MS H   + +G  + +KGP G  +Y  
Sbjct: 715 YTPTSAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKG 774

Query: 159 --------QPGKVRAFGMLAGGSGITPMYQVARSILENLNDKTQIHLIYANVTLEDILLK 218
                   +P   +   MLAGG+GITP+YQ+ +SIL +  D+T+++++YAN T +DIL++
Sbjct: 775 KGNFLVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVR 834

Query: 219 EELDLLAKRYPDRFQIYYVLNQPPKVWDGGVGFVSKEMIRAHCPAPASDIQI-LRCGPPP 273
           EEL+  A ++ +R +I+YV+    + W    GF+++ ++R H P       + L CGPPP
Sbjct: 835 EELEGWASKHKERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPP 894

The following BLAST results are available for this feature:

BLAST of Carg01271 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027734.1	2.2e-156	100.00	NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6596195.1	6.5e-156	99.64	NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022939629.1	1.4e-155	99.29	NADH--cytochrome b5 reductase 1-like [Cucurbita moschata]	[more]
XP_023540168.1	5.7e-152	97.14	NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022971225.1	2.2e-151	97.14	NADH--cytochrome b5 reductase 1-like [Cucurbita maxima]	[more]

BLAST of Carg01271 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNT1	1.2e-123	77.50	NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=...	[more]
A1DHW1	1.1e-70	50.56	NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3...	[more]
Q4X0B5	3.3e-70	49.81	NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A...	[more]
Q54NC1	4.8e-69	51.25	NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE...	[more]
Q2UFN3	8.1e-69	48.88	NADH-cytochrome b5 reductase 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40...	[more]

BLAST of Carg01271 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FHS1	7.0e-156	99.29	NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111445460 PE=3 ...	[more]
A0A6J1I558	1.0e-151	97.14	NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111470015 PE=3 SV...	[more]
A0A1S3B6Z6	6.8e-143	90.00	NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103486847 PE=3 SV=1	[more]
A0A5D3DPB8	6.8e-143	90.00	NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
E5GBY3	6.8e-143	90.00	NADH-cytochrome b5 reductase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]

BLAST of Carg01271 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17770.1	8.2e-125	77.50	NADH:cytochrome B5 reductase 1	[more]
AT5G20080.1	4.2e-52	43.02	FAD/NAD(P)-binding oxidoreductase	[more]
AT1G37130.1	2.7e-43	37.16	nitrate reductase 2	[more]
AT1G77760.1	7.4e-41	34.51	nitrate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	PRINTS	PR00371	FPNCR	coord: 194..205 score: 43.58 coord: 156..175 score: 37.3 coord: 250..258 score: 37.16
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 194..205 score: 64.06 coord: 156..175 score: 63.33 coord: 250..258 score: 59.49 coord: 133..147 score: 49.17 coord: 97..104 score: 41.93 coord: 75..86 score: 56.08
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 157..263 e-value: 1.3E-29 score: 103.0
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 49..147 e-value: 2.1E-31 score: 108.2
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 38..137 e-value: 3.2E-33 score: 116.3
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..280
None	No IPR available	PANTHER	PTHR19370:SF203	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..280
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 49..280 e-value: 1.9062E-115 score: 329.528
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 138..280 e-value: 4.2E-43 score: 148.6
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 136..276
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 44..148 score: 13.285454
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 41..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01271-RA	Carg01271-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0016491	oxidoreductase activity