Carg01194 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01194
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	bifunctional nuclease 2
Location	Carg_Chr06: 742474 .. 745727 (+)
RNA-Seq Expression	Carg01194
Synteny	Carg01194

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAATCTTCTCTCTTTCTTCTTTCTCAATTGATTCAGGGGATTCAAACATTCTCTTTCTTCTCTCTCTCTACACTTCACGTTTCCCTTTCAATCTTCTTCATCTTCGTCTTTAATCTCCACTTTTCTCTGTAATTCCGCCATTACAATGCTGGGTCTAACCTTCCACTTCTGTGTTCCCTCCATTTTTCCCCGATCCGCCATAGCCATGGATCACCCTTCTACCTCCTCTTCCCTCCACTTCTCTCTCCTTCGATCGCGATCCATTGCTTTTCGCTCTCGCCGCTCCAACTCGATACTCATTTCTTGCCACTCTTCTCCCGACAGATTCGCCCACACTGATGATGACTCCCGTCGGGATTATTTTGAAGCTTCGTTTCTCGTCACAGGTTCTTAATCTTTCACTTGGCTACTTTTTTGTTTTACTTTGATGTTTGTTGCAATTGCCGATGAATCGATGGGGTGTTTTGTGATTTCTGTGGTTGTTTGTGTATTACTGACTGTTAGAGACTATCAATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGATGCGAGTTTCAATCAGAGAGAATCGCGAGTCAATTCCCATTCACTTGGATTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTTCTCAAGATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGTATGTTGGAGAAAAACTGTGACGTGATTCGGTTTTGTGTTTTCTTATGAATTTGTCAAAAAGAAGCTGCAAATTTTGGACTTGGTTTGCAGGGGAATATGCTATTGAGAAACTTATAGATTGTCAGCTTGGGATTGAAAATGGGGTGGGTTTTCTCTGGTTTTGTTCTTCATATATATATATATTCATATATGTGTAGCTACCTTGTTGTTGGTATTAATTCTTAAATGGTCTCATATTACAGGATGCCCCTGATCTCTTCCAGTTTATACAAGACCTCATTGTGAAAGTTGGCTATCAAGTATGAACTGACTTCTAACATACTGAATAAATATATGCTTAGGATTTATTGTTTATCAGTCTTTGATGGGGAGGATTTATGAAACAAATGTTTTTTTTCGAGAGTTCAATAGGGGTTTGAACTCTTGACCTCTTAGTCGAGGATATATGTCTTAACTAGTTGAGCGATTCTTGAACTGAGGTTTGTGACATTAATGTTAAGAACATTAGGATGGAATTTTTTTTTTTTTTTCAGATTTTGATTCTTCAATAGCTTGAAAAGTGAATTGTTGCTTGCTACAATCATACATGGAATAATCAAGAAATATATACATGGCTTGTTAGTTTTTTCTATACAAGAACCAATGAGCTGCAAAAATCTGCTAGTTTTAATGTTGCTTTGCGATGTATGTTTACCTGATAGGACAAGCTTATGCTCGAGCAAGAATATTAGTGTCTTTGCCCAGTTGTGAGATCCCACATCCGTTGGAGAGGGGAACACAACATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGCAGATGCGTTTTAGAATTGTGAGGCTGACAGCGATACGTAACGAGCCAAAACGGACATCGGGCAATGTGCCAGCGAGGACGTTGAGCCCCCAAGGGGGGGTGGATTATGAGATTCCACATATGTTGGAGAGGGGAACGAAGCATTCCTTATAAGAGTGTGGAAACCTCTCCCTAGCAGATGCGTTTTAAAATTGTGAGGCTGACGGTGATACGCAACAGGCGAAAGCGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACACTTGCTTTCGAGGAATTATATAATTTGGGATTTAATTCTTCATCTAGTCATAGAATTATTTTCTCTAAGCTATATTATGGGTGGGCAGTGATTACTGATAGGAACCCTCCTTCCTTCACTTGTTTCTTTAATGTAATTGAGGATGAAAATCTAGAAGCACTGCACAGTTTCTGAATAAATCATTTGTACTTCTCATGTTCATTTCCTTTTTTGATAACCATGAAAAGGGGTTTAGAGCGATAATCTAATGCCATTTTATATTATCACGAGATAGGTCACCACAGCGAGAATTACTGAACGAGTGATGAACACTTACTTCGCCCGGCTCTTCTTGAAAAAGGTTAGCTCTTATAAACTCATTCACAATCAGCCAATACATTTTTAGAAGTTCTCAATACATGCCTTGTCTAACTATTATCATCTGCAGGATGGGGAAAATGAAACGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTAAATGTTGCATACCGATGTAAGGTAGATATTCTACCATTAACTGCTGGGATCCTTGCAGAACTTCTACTAGTCGCTAGAATGACTTAGATTCTTTACTTTGCACCCTCTCCGCCCGATTTCATTCTCTTGACCGAAATAATTTCTTACTTCACTGAAAGTTTGCTGCTCACGGTGTTTTTCGGTATAACAAATGTGCAGATACCTGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCCATTAGAGTTAGTTATGGGTTTGGAAGAGTGCGTGAGAGGAAATCATGTTATGACGTTCTACTCGACAGGTAGAAGTGCATGTATTGTTTGCTATTTGATGAACATCTTTTCTTGATATCCAAAAACAATCATGTTCGTTTCGATCAGTGCTGCTGATGGTCCGGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATGGCTATTTACGAAGAACGGTACAAAGATGCAGGTATCTATCCAAACATTTTGTTCTTTATCATGTTTCTACAAATGAGTGTATTTATTTATAGCTCATGTTTGTTTCCTTCAGCCTTGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCCGAAGACGAGGAGTAACTCTGTGTAATCGACTGCTCTAAAAAGAGGAATCGTCGTGTACACGACGAAACATGTCTTAATTTTTCTGATACTGCACCCTAAACAGGGCGACGTTTCGGTCTCTTGGAACATACAATTCAGCATTGGATGAATTGGCAACGCTGAGCAAAGCGATGCGCATCCGTGCAGAAAAATTTCGATAGTTATGGTGGTCGTCGGGCTGTACAGTTACTGTTGAGAGAGAGTGTAAATATTACATTTGGGTAGCTAATGTGTTGTATCTCTTGATATGAATGAGGTGGAGATGGCTGATTCTTTTTACACGGCGAGCTGTAAGTTTGTAAGGTTGCAGAGAATGCTAATGTGAAAAGGATGCTTTTGTATGTCCTAACAAAGTCTCATTCCTTTGATCCTT

mRNA sequence

CAAATCTTCTCTCTTTCTTCTTTCTCAATTGATTCAGGGGATTCAAACATTCTCTTTCTTCTCTCTCTCTACACTTCACGTTTCCCTTTCAATCTTCTTCATCTTCGTCTTTAATCTCCACTTTTCTCTGTAATTCCGCCATTACAATGCTGGGTCTAACCTTCCACTTCTGTGTTCCCTCCATTTTTCCCCGATCCGCCATAGCCATGGATCACCCTTCTACCTCCTCTTCCCTCCACTTCTCTCTCCTTCGATCGCGATCCATTGCTTTTCGCTCTCGCCGCTCCAACTCGATACTCATTTCTTGCCACTCTTCTCCCGACAGATTCGCCCACACTGATGATGACTCCCGTCGGGATTATTTTGAAGCTTCGTTTCTCGTCACAGAGACTATCAATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGATGCGAGTTTCAATCAGAGAGAATCGCGAGTCAATTCCCATTCACTTGGATTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTTCTCAAGATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGGGAATATGCTATTGAGAAACTTATAGATTGTCAGCTTGGGATTGAAAATGGGGATGCCCCTGATCTCTTCCAGTTTATACAAGACCTCATTGTGAAAGTTGGCTATCAAGTCACCACAGCGAGAATTACTGAACGAGTGATGAACACTTACTTCGCCCGGCTCTTCTTGAAAAAGGATGGGGAAAATGAAACGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTAAATGTTGCATACCGATGTAAGATACCTGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCCATTAGAGTTAGTTATGGGTTTGGAAGAGTGCGTGAGAGGAAATCATGTTATGACGTTCTACTCGACAGTGCTGCTGATGGTCCGGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATGGCTATTTACGAAGAACGGTACAAAGATGCAGCCTTGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCCGAAGACGAGGAGTAACTCTGTGTAATCGACTGCTCTAAAAAGAGGAATCGTCGTGTACACGACGAAACATGTCTTAATTTTTCTGATACTGCACCCTAAACAGGGCGACGTTTCGGTCTCTTGGAACATACAATTCAGCATTGGATGAATTGGCAACGCTGAGCAAAGCGATGCGCATCCGTGCAGAAAAATTTCGATAGTTATGGTGGTCGTCGGGCTGTACAGTTACTGTTGAGAGAGAGTGTAAATATTACATTTGGGTAGCTAATGTGTTGTATCTCTTGATATGAATGAGGTGGAGATGGCTGATTCTTTTTACACGGCGAGCTGTAAGTTTGTAAGGTTGCAGAGAATGCTAATGTGAAAAGGATGCTTTTGTATGTCCTAACAAAGTCTCATTCCTTTGATCCTT

Coding sequence (CDS)

ATGCTGGGTCTAACCTTCCACTTCTGTGTTCCCTCCATTTTTCCCCGATCCGCCATAGCCATGGATCACCCTTCTACCTCCTCTTCCCTCCACTTCTCTCTCCTTCGATCGCGATCCATTGCTTTTCGCTCTCGCCGCTCCAACTCGATACTCATTTCTTGCCACTCTTCTCCCGACAGATTCGCCCACACTGATGATGACTCCCGTCGGGATTATTTTGAAGCTTCGTTTCTCGTCACAGAGACTATCAATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGATGCGAGTTTCAATCAGAGAGAATCGCGAGTCAATTCCCATTCACTTGGATTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTTCTCAAGATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGGGAATATGCTATTGAGAAACTTATAGATTGTCAGCTTGGGATTGAAAATGGGGATGCCCCTGATCTCTTCCAGTTTATACAAGACCTCATTGTGAAAGTTGGCTATCAAGTCACCACAGCGAGAATTACTGAACGAGTGATGAACACTTACTTCGCCCGGCTCTTCTTGAAAAAGGATGGGGAAAATGAAACGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTAAATGTTGCATACCGATGTAAGATACCTGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCCATTAGAGTTAGTTATGGGTTTGGAAGAGTGCGTGAGAGGAAATCATGTTATGACGTTCTACTCGACAGTGCTGCTGATGGTCCGGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATGGCTATTTACGAAGAACGGTACAAAGATGCAGCCTTGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCCGAAGACGAGGAGTAA

Protein sequence

MLGLTFHFCVPSIFPRSAIAMDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKSEDEE

Homology

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BLAST of Carg01194 vs. NCBI nr
Match: XP_022951431.1 (bifunctional nuclease 2 [Cucurbita moschata] >KAG7027810.1 Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 612.5 bits (1578), Expect = 2.1e-171
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDR 60
           MLGLTFHFCVPSIFPRSAIAMDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDR
Sbjct: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDR 60

Query: 61  FAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFR 120
           FAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFR
Sbjct: 61  FAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFR 120

Query: 121 SPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTT 180
           SPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTT
Sbjct: 121 SPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTT 180

Query: 181 ARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 240
           ARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV
Sbjct: 181 ARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 240

Query: 241 SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKL 300
           SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKL
Sbjct: 241 SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKL 300

Query: 301 RKSEDEE 308
           RKSEDEE
Sbjct: 301 RKSEDEE 307

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BLAST of Carg01194 vs. NCBI nr
Match: XP_022971524.1 (bifunctional nuclease 2-like [Cucurbita maxima])

HSP 1 Score: 581.3 bits (1497), Expect = 5.1e-162
Identity = 295/311 (94.86%), Postives = 299/311 (96.14%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPST----SSSLHFSLLRSRSIAFRSRRSNSILISCHS 60
           MLGLTFHFCVPSIFPRSAIAMDHPST    SS LHFSL RS SIA RSRRSNSILISCHS
Sbjct: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60

Query: 61  SPDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFF 120
           SPD FAHTDDDS RDYFEASFLVTETINHYRMWKKRFQEDASFNQRES+VNSHSLGLGFF
Sbjct: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120

Query: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180
           RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY
Sbjct: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180

Query: 181 QVTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240
           QVTTARITERVMNTYFARLFLKKDGE ETLSVDARPSDALNVAYRCKIPVLVSKQIVYED
Sbjct: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240

Query: 241 AIRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDK 300
           AIRVSYGFGRVRERKSCYD+LLDSAADGP+FLSEELDMLKNMKMAIYEERYKDAA+WRDK
Sbjct: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300

Query: 301 LTKLRKSEDEE 308
           LTKLRKSE EE
Sbjct: 301 LTKLRKSEHEE 311

[Close]

BLAST of Carg01194 vs. NCBI nr
Match: KAG6596262.1 (Bifunctional nuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 563.9 bits (1452), Expect = 8.4e-157
Identity = 282/283 (99.65%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 21  MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT 80
           MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT
Sbjct: 1   MDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEASFLVT 60

Query: 81  ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV 140
           ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV
Sbjct: 61  ETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVV 120

Query: 141 GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD 200
           GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD
Sbjct: 121 GEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKD 180

Query: 201 GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS 260
           GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS
Sbjct: 181 GENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDS 240

Query: 261 AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKS 304
           AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRK+
Sbjct: 241 AADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKA 283

[Close]

BLAST of Carg01194 vs. NCBI nr
Match: XP_038905904.1 (bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nuclease 2 [Benincasa hispida] >XP_038905906.1 bifunctional nuclease 2 [Benincasa hispida])

HSP 1 Score: 525.0 bits (1351), Expect = 4.3e-145
Identity = 268/311 (86.17%), Postives = 284/311 (91.32%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPST---SSSLHFSLLRSRSIAFRSRRSNSILISCHSS 60
           MLGL  HFC P+IFP SAIAMDHPS    SSSLHFS+LRSR I  RSRR  SILISCHSS
Sbjct: 1   MLGLAPHFCFPTIFPGSAIAMDHPSASRPSSSLHFSVLRSRPIPVRSRRPKSILISCHSS 60

Query: 61  PDRFAHT-DDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRE-SRVNSHSLGLGF 120
            DR  H+ DDDS +DYFEASFLV+ETI HYRMWKKRFQEDA+FNQRE SRVNSHSLGLGF
Sbjct: 61  RDRSTHSHDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDATFNQRESSRVNSHSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENG+APD+FQFIQDLIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180

Query: 181 YQVTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYE 240
           Y+ TTARITERV+NTYFARLFL+K+GENE LSVDARPSDALN+AYRCKIPVLVSKQIV+E
Sbjct: 181 YEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRD 300
           D IRVSYGFGRV ERKSC+DVLLD AADGPDFLSEELDMLKNMKMAIYEERYKDAA+WRD
Sbjct: 241 DGIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRD 300

Query: 301 KLTKLRKSEDE 307
           KLTKLRKS  E
Sbjct: 301 KLTKLRKSVHE 311

[Close]

BLAST of Carg01194 vs. NCBI nr
Match: XP_022965292.1 (bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965293.1 bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965294.1 bifunctional nuclease 2-like [Cucurbita maxima])

HSP 1 Score: 516.9 bits (1330), Expect = 1.2e-142
Identity = 262/309 (84.79%), Postives = 282/309 (91.26%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPST---SSSLHFSLLRSRSIAFRSRRSNSILISCHSS 60
           MLGL  HF + ++FP SAIAMDHPS    SSSLHFS+LRSR  A RSRR  SIL+SCHSS
Sbjct: 1   MLGLVPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILVSCHSS 60

Query: 61  PDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFR 120
            DR  H+DDDS +DYFEASFLV+ETI HYRMWKKRFQED +FNQ ESRVNS SLGLGFFR
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFFR 120

Query: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQ 180
           RF+SPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENG+APDLFQFIQDL+VKVGY+
Sbjct: 121 RFQSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 VTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDA 240
           VTTARITERV+NTYFARLFL+KDGENETLSVDARPSDALN+AYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKL 300
           IRVS+GFGRVR+RKSC+DVLLD AADGPDFLSEELDMLKNMKMAIYEERYKDAA+WRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

Query: 301 TKLRKSEDE 307
           TKLRKS  E
Sbjct: 301 TKLRKSVHE 309

[Close]

BLAST of Carg01194 vs. ExPASy Swiss-Prot
Match: Q93VH2 (Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 6.9e-29
Identity = 70/183 (38.25%), Postives = 111/183 (60.66%), Query Frame = 0


Query: 124 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 183
           + LK+      LLPI+V E     L+     ++    P ++Q ++D++ K+GY+V   R+
Sbjct: 140 IVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKDMVDKMGYEVRLVRV 199

Query: 184 TERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSY 243
           T RV   YFA L+L K G+ ++ +S D RPSDA+N+A RCK+P+ V+K + Y D +RV  
Sbjct: 200 TTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-I 259

Query: 244 GFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDKLT 302
             G++ ++    D LL +  D P+    F ++E D+++NM  A+ EERY +AA WRDKL 
Sbjct: 260 DSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLG 319

[Close]

BLAST of Carg01194 vs. ExPASy Swiss-Prot
Match: Q6YZM6 (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-28
Identity = 75/201 (37.31%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 106 VNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQ 165
           V+++S G         P + L+I    + LLPI+V E     L+     +     P ++Q
Sbjct: 120 VHNNSQGRNIPESAPQPAIVLRIEDGSETLLPIIVLEMPSVLLMAAIRNVHIA-RPTIYQ 179

Query: 166 FIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGEN-ETLSVDARPSDALNVAYRCKI 225
            ++++I K+GY+V   RI +R+   Y A LFL K G++ E+++ D RPSDA+N+A RCK+
Sbjct: 180 VVKEMIDKMGYEVKLVRINKRIQEAYCAELFLTKVGDHTESITFDLRPSDAINIAVRCKV 239

Query: 226 PVLVSKQIVYEDAIRVSYGFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKM 285
           P+ V + + Y D IR S    R+       D LL +  D PD      ++E  +++NM +
Sbjct: 240 PIQVHRSLAYSDGIR-SVEPARMAIAAGMSDGLLFTELDRPDGQPCVEAQEFGLIRNMLI 299

Query: 286 AIYEERYKDAALWRDKLTKLR 302
           A  EERYKDAA WRDKL  LR
Sbjct: 300 AAVEERYKDAATWRDKLMLLR 318

[Close]

BLAST of Carg01194 vs. ExPASy Swiss-Prot
Match: Q9FWS6 (Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.8e-28
Identity = 71/185 (38.38%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 122 PTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTA 181
           P + LK+      LLPI+V E     L+     ++    P ++Q +++++ K+GY+V   
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKEMVDKMGYEVRLV 193

Query: 182 RITERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 241
           R+T+RV   YFA+LFL K G  +E +S D RPSDA+N+A RCKIP+ V+K + Y D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 242 SYGFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDK 301
               G++       D LL +  D P+      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 IES-GKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

[Close]

BLAST of Carg01194 vs. ExPASy Swiss-Prot
Match: Q5N8J3 (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-26
Identity = 69/182 (37.91%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 124 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 183
           + LK+    D LLPI+V E     L+     I     P ++  ++++  ++GY V   RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 184 TERVMNTYFARLFLKKDG-ENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV-- 243
           TE V + Y++RL+L K G E ET+S+D +PSDA+N+A+RCK+P+ V+++I Y + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 244 -SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTK 302
            +     V   +  Y   LD   D P F ++E D+++NM +A  EERYKDAA +RD+L  
Sbjct: 265 PTPSESYVSSDQFQY-TRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFM 324

[Close]

BLAST of Carg01194 vs. ExPASy Swiss-Prot
Match: Q09LL3 (Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-26
Identity = 69/182 (37.91%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 124 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 183
           + LK+    D LLPI+V E     L+     I     P ++  ++++  ++GY V   RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 184 TERVMNTYFARLFLKKDG-ENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV-- 243
           TE V + Y++RL+L K G E ET+S+D +PSDA+N+A+RCK+P+ V+++I Y + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 244 -SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTK 302
            +     V   +  Y   LD   D P F ++E D+++NM +A  EERYKDAA +RD+L  
Sbjct: 265 PTPSESYVSSDQFQY-TRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFM 324

[Close]

BLAST of Carg01194 vs. ExPASy TrEMBL
Match: A0A6J1GHK4 (bifunctional nuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111454246 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 9.9e-172
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDR 60
           MLGLTFHFCVPSIFPRSAIAMDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDR
Sbjct: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDR 60

Query: 61  FAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFR 120
           FAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFR
Sbjct: 61  FAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFR 120

Query: 121 SPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTT 180
           SPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTT
Sbjct: 121 SPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTT 180

Query: 181 ARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 240
           ARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV
Sbjct: 181 ARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 240

Query: 241 SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKL 300
           SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKL
Sbjct: 241 SYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKL 300

Query: 301 RKSEDEE 308
           RKSEDEE
Sbjct: 301 RKSEDEE 307

[Close]

BLAST of Carg01194 vs. ExPASy TrEMBL
Match: A0A6J1I8T8 (bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470219 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.5e-162
Identity = 295/311 (94.86%), Postives = 299/311 (96.14%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPST----SSSLHFSLLRSRSIAFRSRRSNSILISCHS 60
           MLGLTFHFCVPSIFPRSAIAMDHPST    SS LHFSL RS SIA RSRRSNSILISCHS
Sbjct: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60

Query: 61  SPDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFF 120
           SPD FAHTDDDS RDYFEASFLVTETINHYRMWKKRFQEDASFNQRES+VNSHSLGLGFF
Sbjct: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120

Query: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180
           RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY
Sbjct: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180

Query: 181 QVTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240
           QVTTARITERVMNTYFARLFLKKDGE ETLSVDARPSDALNVAYRCKIPVLVSKQIVYED
Sbjct: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240

Query: 241 AIRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDK 300
           AIRVSYGFGRVRERKSCYD+LLDSAADGP+FLSEELDMLKNMKMAIYEERYKDAA+WRDK
Sbjct: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300

Query: 301 LTKLRKSEDEE 308
           LTKLRKSE EE
Sbjct: 301 LTKLRKSEHEE 311

[Close]

BLAST of Carg01194 vs. ExPASy TrEMBL
Match: A0A6J1HJX9 (bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.7e-143
Identity = 262/309 (84.79%), Postives = 282/309 (91.26%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPST---SSSLHFSLLRSRSIAFRSRRSNSILISCHSS 60
           MLGL  HF + ++FP SAIAMDHPS    SSSLHFS+LRSR  A RSRR  SIL+SCHSS
Sbjct: 1   MLGLVPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILVSCHSS 60

Query: 61  PDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFR 120
            DR  H+DDDS +DYFEASFLV+ETI HYRMWKKRFQED +FNQ ESRVNS SLGLGFFR
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFFR 120

Query: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQ 180
           RF+SPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENG+APDLFQFIQDL+VKVGY+
Sbjct: 121 RFQSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 VTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDA 240
           VTTARITERV+NTYFARLFL+KDGENETLSVDARPSDALN+AYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKL 300
           IRVS+GFGRVR+RKSC+DVLLD AADGPDFLSEELDMLKNMKMAIYEERYKDAA+WRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

Query: 301 TKLRKSEDE 307
           TKLRKS  E
Sbjct: 301 TKLRKSVHE 309

[Close]

BLAST of Carg01194 vs. ExPASy TrEMBL
Match: A0A6J1E735 (bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 3.7e-142
Identity = 261/309 (84.47%), Postives = 281/309 (90.94%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPST---SSSLHFSLLRSRSIAFRSRRSNSILISCHSS 60
           MLGL  HF + ++FP SAIAMDHPS    SSSLHFS+LRSR  A  SRR  SIL+SCHSS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60

Query: 61  PDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFR 120
            DR  H+DDDS +DYFEASFLV+ETI HYRMWKKRFQEDA+FNQ ESRVNS  LGLGFFR
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPLLGLGFFR 120

Query: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQ 180
           RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENG+APDLFQFIQDL+VKVGY+
Sbjct: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 VTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDA 240
           VTTARITERV+NTYFARLFL+KDGENETLSVDARPSDALN+AYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKL 300
           IRVS+GFGRVR+RKSC+DVLLD AADGPDFLSEELDMLKNMKMAIYEERYKDAA+WRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

Query: 301 TKLRKSEDE 307
           T+LRKS  E
Sbjct: 301 TRLRKSVHE 309

[Close]

BLAST of Carg01194 vs. ExPASy TrEMBL
Match: A0A0A0L3L8 (BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 8.2e-142
Identity = 262/311 (84.24%), Postives = 281/311 (90.35%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPST---SSSLHFSLLRSRSIAFRSRRSNSILISCHSS 60
           MLGL  HFC+P+IF  SAIAMDHPS    S SLHFSL RSR  A RSRR  SILISCHSS
Sbjct: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  PDRFAHTDDD--SRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGF 120
            DR  H+DDD  S +DYFEASFLV+ETI HYRMWKKRFQEDA+FNQRESR NS+SLGLGF
Sbjct: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENG+APD+FQFIQDLIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180

Query: 181 YQVTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYE 240
           Y+  TARITERV+NTYFARLFL+K+GE+E LSVDARPSDALN+AYRCKIPVLVSKQIV+E
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRD 300
           DAIRVSYGFGRV ERKSC+DVLLD AADGPDFLSEELDMLKNMK+AIYEERYKDAA+WRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

Query: 301 KLTKLRKSEDE 307
           KLTKLRKS  E
Sbjct: 301 KLTKLRKSVHE 311

[Close]

BLAST of Carg01194 vs. TAIR 10
Match: AT5G66050.1 (Wound-responsive family protein )

HSP 1 Score: 236.1 bits (601), Expect = 3.7e-62
Identity = 130/302 (43.05%), Postives = 186/302 (61.59%), Query Frame = 0


Query: 14  FPRSAIAMDHPSTSSSLHFSLLRSRSI-AFRSRRSNSILISCHSSPDRFAHTDDDSRRDY 73
           F RS  A    S+SS   F      ++ + RS R   I   C            D   ++
Sbjct: 24  FSRSISASLSSSSSSPSSFRCCTPLNLKSLRSHRRPKISHICCDGSSISGSGLGDPDLEF 83

Query: 74  FEASFLVTETINHYRMWKKRFQEDA-----------SFNQRESRVNSHSLGLGFFRRFRS 133
            +AS LV ET  HY+M +  F++D+           S    ESRV    +GLGF R+F+ 
Sbjct: 84  LQASVLVAETSMHYKMRRHGFRQDSMWQTSRPLPPFSIRASESRVGVLPIGLGFLRQFKQ 143

Query: 134 PTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTA 193
           PT+FLKISC+GD+LLP++VG+ A+EKL+D  L     + PD FQF+  ++ K+GY+V   
Sbjct: 144 PTIFLKISCDGDYLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGYEVKMV 203

Query: 194 RITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVS 253
           ++T R++NTY+A L L K G+ E + +D+RPSDA+NVA  C+ P+ V+K IV E+AI++ 
Sbjct: 204 KLTGRIVNTYYASLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEEAIKIG 263

Query: 254 YGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLR 304
           YG GR +  K  ++V+LDSA DGPD LSEEL +++NM +A  EERY DAA+WRD+L  L+
Sbjct: 264 YG-GRPQSAKPVFNVILDSAPDGPDPLSEELKLVRNMDLASKEERYIDAAMWRDRLKNLQ 323

[Close]

BLAST of Carg01194 vs. TAIR 10
Match: AT5G66050.2 (Wound-responsive family protein )

HSP 1 Score: 224.9 bits (572), Expect = 8.6e-59
Identity = 110/230 (47.83%), Postives = 159/230 (69.13%), Query Frame = 0


Query: 85  HYRMWKKRFQEDA-----------SFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGD 144
           HY+M +  F++D+           S    ESRV    +GLGF R+F+ PT+FLKISC+GD
Sbjct: 2   HYKMRRHGFRQDSMWQTSRPLPPFSIRASESRVGVLPIGLGFLRQFKQPTIFLKISCDGD 61

Query: 145 FLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFA 204
           +LLP++VG+ A+EKL+D  L     + PD FQF+  ++ K+GY+V   ++T R++NTY+A
Sbjct: 62  YLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGYEVKMVKLTGRIVNTYYA 121

Query: 205 RLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSC 264
            L L K G+ E + +D+RPSDA+NVA  C+ P+ V+K IV E+AI++ YG GR +  K  
Sbjct: 122 SLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEEAIKIGYG-GRPQSAKPV 181

Query: 265 YDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKS 304
           ++V+LDSA DGPD LSEEL +++NM +A  EERY DAA+WRD+L  L+ S
Sbjct: 182 FNVILDSAPDGPDPLSEELKLVRNMDLASKEERYIDAAMWRDRLKNLQNS 230

[Close]

BLAST of Carg01194 vs. TAIR 10
Match: AT1G19660.1 (Wound-responsive family protein )

HSP 1 Score: 129.4 bits (324), Expect = 4.9e-30
Identity = 70/183 (38.25%), Postives = 111/183 (60.66%), Query Frame = 0


Query: 124 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 183
           + LK+      LLPI+V E     L+     ++    P ++Q ++D++ K+GY+V   R+
Sbjct: 140 IVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKDMVDKMGYEVRLVRV 199

Query: 184 TERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSY 243
           T RV   YFA L+L K G+ ++ +S D RPSDA+N+A RCK+P+ V+K + Y D +RV  
Sbjct: 200 TTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-I 259

Query: 244 GFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDKLT 302
             G++ ++    D LL +  D P+    F ++E D+++NM  A+ EERY +AA WRDKL 
Sbjct: 260 DSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLG 319

[Close]

BLAST of Carg01194 vs. TAIR 10
Match: AT1G19660.2 (Wound-responsive family protein )

HSP 1 Score: 129.4 bits (324), Expect = 4.9e-30
Identity = 70/183 (38.25%), Postives = 111/183 (60.66%), Query Frame = 0


Query: 124 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 183
           + LK+      LLPI+V E     L+     ++    P ++Q ++D++ K+GY+V   R+
Sbjct: 140 IVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKDMVDKMGYEVRLVRV 199

Query: 184 TERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSY 243
           T RV   YFA L+L K G+ ++ +S D RPSDA+N+A RCK+P+ V+K + Y D +RV  
Sbjct: 200 TTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-I 259

Query: 244 GFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDKLT 302
             G++ ++    D LL +  D P+    F ++E D+++NM  A+ EERY +AA WRDKL 
Sbjct: 260 DSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLG 319

[Close]

BLAST of Carg01194 vs. TAIR 10
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 126.3 bits (316), Expect = 4.2e-29
Identity = 71/185 (38.38%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 122 PTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTA 181
           P + LK+      LLPI+V E     L+     ++    P ++Q +++++ K+GY+V   
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKEMVDKMGYEVRLV 193

Query: 182 RITERVMNTYFARLFLKKDGE-NETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 241
           R+T+RV   YFA+LFL K G  +E +S D RPSDA+N+A RCKIP+ V+K + Y D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 242 SYGFGRVRERKSCYDVLLDSAADGPD----FLSEELDMLKNMKMAIYEERYKDAALWRDK 301
               G++       D LL +  D P+      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 IES-GKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

The following BLAST results are available for this feature:

BLAST of Carg01194 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951431.1	2.1e-171	100.00	bifunctional nuclease 2 [Cucurbita moschata] >KAG7027810.1 Bifunctional nuclease...	[more]
XP_022971524.1	5.1e-162	94.86	bifunctional nuclease 2-like [Cucurbita maxima]	[more]
KAG6596262.1	8.4e-157	99.65	Bifunctional nuclease 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038905904.1	4.3e-145	86.17	bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nucleas...	[more]
XP_022965292.1	1.2e-142	84.79	bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965293.1 bifunctional nuc...	[more]

BLAST of Carg01194 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VH2	6.9e-29	38.25	Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1	[more]
Q6YZM6	2.0e-28	37.31	Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV...	[more]
Q9FWS6	5.8e-28	38.38	Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1	[more]
Q5N8J3	1.1e-26	37.91	Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV...	[more]
Q09LL3	1.1e-26	37.91	Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1	[more]

BLAST of Carg01194 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GHK4	9.9e-172	100.00	bifunctional nuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111454246 PE=3 SV=1	[more]
A0A6J1I8T8	2.5e-162	94.86	bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470219 PE=3 SV...	[more]
A0A6J1HJX9	5.7e-143	84.79	bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV...	[more]
A0A6J1E735	3.7e-142	84.47	bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 ...	[more]
A0A0A0L3L8	8.2e-142	84.24	BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV...	[more]

BLAST of Carg01194 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66050.1	3.7e-62	43.05	Wound-responsive family protein	[more]
AT5G66050.2	8.6e-59	47.83	Wound-responsive family protein	[more]
AT1G19660.1	4.9e-30	38.25	Wound-responsive family protein	[more]
AT1G19660.2	4.9e-30	38.25	Wound-responsive family protein	[more]
AT1G75380.1	4.2e-29	38.38	bifunctional nuclease in basal defense response 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003729	Bifunctional nuclease domain	PFAM	PF02577	DUF151	coord: 133..240 e-value: 6.9E-17 score: 61.3
IPR003729	Bifunctional nuclease domain	PROSITE	PS51658	BFN	coord: 103..240 score: 27.090006
IPR036104	Bifunctional nuclease superfamily	GENE3D	3.10.690.10	Bifunctional nuclease domain	coord: 96..243 e-value: 3.5E-28 score: 100.0
IPR036104	Bifunctional nuclease superfamily	SUPERFAMILY	103256	Hypothetical protein TM0160	coord: 111..241
None	No IPR available	PANTHER	PTHR15160	VON HIPPEL-LINDAU PROTEIN	coord: 24..300
None	No IPR available	PANTHER	PTHR15160:SF1	VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR-RELATED	coord: 24..300

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01194-RA	Carg01194-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030891	VCB complex
molecular_function	GO:0004518	nuclease activity