Carg01101 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01101
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionlactosylceramide 4-alpha-galactosyltransferase-like
LocationCarg_Chr06: 1215347 .. 1216567 (+)
RNA-Seq ExpressionCarg01101
SyntenyCarg01101
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGAAAGGTGTACTGTATCACCCATATTGCCAACCATCTTGCTTGCTGCCATCATCATCTTTCTATGTGGAGATGGCATAATCTACAAAGCTTCGATTCGAACAGTATCCATAAATGAGAACAAAAATTCAAACTCAACAATTACGTGTGTAACACAAGAGCCACTGAAGTCCTCGCCAGAAAACAGAGAAGAAGATGATGATAGTGGAACTTTTGATTCCTTGATAGTTCCATTCAATCTTTCAGGAAAAAAGGCAAGAATTGATTGGTTTAGGAAACACCTTCCCGACTTTCGAATTTTGCAGTCAAACAATTTCACGCAGCAGTTCCATAATCGACTTCAGGAGTTTCTCAGCCATGATTGTGAGACACAATTCTTCATGACATGGATATCTCCAACAAGGACTTTCAGAAGAAGAGAACTACTGGCAGCAGAAAGCATTTTCAAGTCTCACCCCCGTGGATGCTTAACTATTCTATCAAGAACGCTGGATTCAGAAAGAGGCTACAAGATTCTTCAACCTCTACTTGATCATGGGTTTAAAATTCTGGCAATCACACCGGACTTGCCTTCTCTATTCAAAAACACCCCTGTTGAGACTTGGTTTGATGAGATGAAGAGAGGAAAAAAAGACCCAGGTCAGATTCCATTAGCTCAGAATCTATCTAACCTGATGAGACTTGCAGTCCTATACAAGTACGGAGGTGTTTACATAGATACGGATTTTATTGTCTTGAAGTCTTTCATGGGCTTGAAGAACTGTATTGGCGCTCAAAGTATTGATCCGGTATCTAAGAACTGGACTAGACTGAATAATGCTGTTCTTGTTTTTGATAAGAAGCATCCACTTCTAGAGAAATTCATGGAAAATTTTGCATCGAATTTTGATGGGAGCAGATGGGGATATAATGGACCTTTTCTGGTCTCCAGAGTAATAGCAAAGATAACCGGAGCCGGAGCTAGACCAGGTTTTAACGTCACCATATTGTCTCCTGCAGCATTCTACCCAGTGGACTGGACCAAGATTGGTGAACTTTTTAAGAAGCCAGGAAACCGAGCTTCTGCAAATTGGGCAAAAGCAATGCTAGCTCAGCTAAATGACGAGGCTTATGGAATACACCTATGGAACAAGCAGAGTAAAAGCTATACCATTCAGAAGGGAAGTGTCATAGAAAGATTAGTTTCTGCTCACTGCATCATTTGCAAATACACTTAG

mRNA sequence

ATGTCTGAAAGGTGTACTGTATCACCCATATTGCCAACCATCTTGCTTGCTGCCATCATCATCTTTCTATGTGGAGATGGCATAATCTACAAAGCTTCGATTCGAACAGTATCCATAAATGAGAACAAAAATTCAAACTCAACAATTACGTGTGTAACACAAGAGCCACTGAAGTCCTCGCCAGAAAACAGAGAAGAAGATGATGATAGTGGAACTTTTGATTCCTTGATAGTTCCATTCAATCTTTCAGGAAAAAAGGCAAGAATTGATTGGTTTAGGAAACACCTTCCCGACTTTCGAATTTTGCAGTCAAACAATTTCACGCAGCAGTTCCATAATCGACTTCAGGAGTTTCTCAGCCATGATTGTGAGACACAATTCTTCATGACATGGATATCTCCAACAAGGACTTTCAGAAGAAGAGAACTACTGGCAGCAGAAAGCATTTTCAAGTCTCACCCCCGTGGATGCTTAACTATTCTATCAAGAACGCTGGATTCAGAAAGAGGCTACAAGATTCTTCAACCTCTACTTGATCATGGGTTTAAAATTCTGGCAATCACACCGGACTTGCCTTCTCTATTCAAAAACACCCCTGTTGAGACTTGGTTTGATGAGATGAAGAGAGGAAAAAAAGACCCAGGTCAGATTCCATTAGCTCAGAATCTATCTAACCTGATGAGACTTGCAGTCCTATACAAGTACGGAGGTGTTTACATAGATACGGATTTTATTGTCTTGAAGTCTTTCATGGGCTTGAAGAACTGTATTGGCGCTCAAAGTATTGATCCGGTATCTAAGAACTGGACTAGACTGAATAATGCTGTTCTTGTTTTTGATAAGAAGCATCCACTTCTAGAGAAATTCATGGAAAATTTTGCATCGAATTTTGATGGGAGCAGATGGGGATATAATGGACCTTTTCTGGTCTCCAGAGTAATAGCAAAGATAACCGGAGCCGGAGCTAGACCAGGTTTTAACGTCACCATATTGTCTCCTGCAGCATTCTACCCAGTGGACTGGACCAAGATTGGTGAACTTTTTAAGAAGCCAGGAAACCGAGCTTCTGCAAATTGGGCAAAAGCAATGCTAGCTCAGCTAAATGACGAGGCTTATGGAATACACCTATGGAACAAGCAGAGTAAAAGCTATACCATTCAGAAGGGAAGTGTCATAGAAAGATTAGTTTCTGCTCACTGCATCATTTGCAAATACACTTAG

Coding sequence (CDS)

ATGTCTGAAAGGTGTACTGTATCACCCATATTGCCAACCATCTTGCTTGCTGCCATCATCATCTTTCTATGTGGAGATGGCATAATCTACAAAGCTTCGATTCGAACAGTATCCATAAATGAGAACAAAAATTCAAACTCAACAATTACGTGTGTAACACAAGAGCCACTGAAGTCCTCGCCAGAAAACAGAGAAGAAGATGATGATAGTGGAACTTTTGATTCCTTGATAGTTCCATTCAATCTTTCAGGAAAAAAGGCAAGAATTGATTGGTTTAGGAAACACCTTCCCGACTTTCGAATTTTGCAGTCAAACAATTTCACGCAGCAGTTCCATAATCGACTTCAGGAGTTTCTCAGCCATGATTGTGAGACACAATTCTTCATGACATGGATATCTCCAACAAGGACTTTCAGAAGAAGAGAACTACTGGCAGCAGAAAGCATTTTCAAGTCTCACCCCCGTGGATGCTTAACTATTCTATCAAGAACGCTGGATTCAGAAAGAGGCTACAAGATTCTTCAACCTCTACTTGATCATGGGTTTAAAATTCTGGCAATCACACCGGACTTGCCTTCTCTATTCAAAAACACCCCTGTTGAGACTTGGTTTGATGAGATGAAGAGAGGAAAAAAAGACCCAGGTCAGATTCCATTAGCTCAGAATCTATCTAACCTGATGAGACTTGCAGTCCTATACAAGTACGGAGGTGTTTACATAGATACGGATTTTATTGTCTTGAAGTCTTTCATGGGCTTGAAGAACTGTATTGGCGCTCAAAGTATTGATCCGGTATCTAAGAACTGGACTAGACTGAATAATGCTGTTCTTGTTTTTGATAAGAAGCATCCACTTCTAGAGAAATTCATGGAAAATTTTGCATCGAATTTTGATGGGAGCAGATGGGGATATAATGGACCTTTTCTGGTCTCCAGAGTAATAGCAAAGATAACCGGAGCCGGAGCTAGACCAGGTTTTAACGTCACCATATTGTCTCCTGCAGCATTCTACCCAGTGGACTGGACCAAGATTGGTGAACTTTTTAAGAAGCCAGGAAACCGAGCTTCTGCAAATTGGGCAAAAGCAATGCTAGCTCAGCTAAATGACGAGGCTTATGGAATACACCTATGGAACAAGCAGAGTAAAAGCTATACCATTCAGAAGGGAAGTGTCATAGAAAGATTAGTTTCTGCTCACTGCATCATTTGCAAATACACTTAG

Protein sequence

MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSSPENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLSHDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDHGFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYIDTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGSRWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWAKAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT
Homology
BLAST of Carg01101 vs. NCBI nr
Match: KAG6596350.1 (Lactosylceramide 4-alpha-galactosyltransferase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027901.1 Lactosylceramide 4-alpha-galactosyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 832.8 bits (2150), Expect = 1.3e-237
Identity = 406/406 (100.00%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60
           MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS
Sbjct: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60

Query: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120
           PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS
Sbjct: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120

Query: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180
           HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH
Sbjct: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180

Query: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240
           GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI
Sbjct: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240

Query: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300
           DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS
Sbjct: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300

Query: 301 RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA 360
           RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA
Sbjct: 301 RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA 360

Query: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 407
           KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT
Sbjct: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 406

BLAST of Carg01101 vs. NCBI nr
Match: XP_022948708.1 (lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita moschata])

HSP 1 Score: 830.5 bits (2144), Expect = 6.3e-237
Identity = 404/406 (99.51%), Postives = 405/406 (99.75%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60
           MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS
Sbjct: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60

Query: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120
           PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS
Sbjct: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120

Query: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180
           HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH
Sbjct: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180

Query: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240
           GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI
Sbjct: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240

Query: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300
           DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS
Sbjct: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300

Query: 301 RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA 360
           RWGYNGPFLVSRVIAK+TGAGARPGFNVTIL PAAFYPVDWTKIGELFKKPGNRASANWA
Sbjct: 301 RWGYNGPFLVSRVIAKVTGAGARPGFNVTILPPAAFYPVDWTKIGELFKKPGNRASANWA 360

Query: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 407
           KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT
Sbjct: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 406

BLAST of Carg01101 vs. NCBI nr
Match: XP_023539712.1 (lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 826.2 bits (2133), Expect = 1.2e-235
Identity = 401/406 (98.77%), Postives = 404/406 (99.51%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60
           MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS
Sbjct: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60

Query: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120
           PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS
Sbjct: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120

Query: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180
           HDCET+FFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH
Sbjct: 121 HDCETRFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180

Query: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240
           GFKILAITPDLPSLF+NTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRL VLYKYGGVYI
Sbjct: 181 GFKILAITPDLPSLFRNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLTVLYKYGGVYI 240

Query: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300
           DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS
Sbjct: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300

Query: 301 RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA 360
           RWGYNGPFLVSRVIAK+TGAGARPGFNVTIL PAAFYPVDWTKIGELFKKPGNRASANWA
Sbjct: 301 RWGYNGPFLVSRVIAKVTGAGARPGFNVTILPPAAFYPVDWTKIGELFKKPGNRASANWA 360

Query: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 407
           KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT
Sbjct: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 406

BLAST of Carg01101 vs. NCBI nr
Match: XP_022971397.1 (lactosylceramide 4-alpha-galactosyltransferase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 816.2 bits (2107), Expect = 1.2e-232
Identity = 394/406 (97.04%), Postives = 402/406 (99.01%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60
           MSERCTVSPILPTILLAAIIIF+CGDG+IYKASIRTVSINENKNSNSTITC+TQEPLKSS
Sbjct: 1   MSERCTVSPILPTILLAAIIIFICGDGMIYKASIRTVSINENKNSNSTITCITQEPLKSS 60

Query: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120
           PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS
Sbjct: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120

Query: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180
           HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLD 
Sbjct: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDR 180

Query: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240
           GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI
Sbjct: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240

Query: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300
           DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS
Sbjct: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300

Query: 301 RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA 360
           RWGYNGPFLVSRVIAK+TGAG RPGFNVTIL PAAFYPVDWTKIGELFKKPGNRASANWA
Sbjct: 301 RWGYNGPFLVSRVIAKVTGAGDRPGFNVTILPPAAFYPVDWTKIGELFKKPGNRASANWA 360

Query: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 407
           KAML+QLND+AYGIHLWNKQSKSYTIQKGSVIE+LVS HCI+CKYT
Sbjct: 361 KAMLSQLNDKAYGIHLWNKQSKSYTIQKGSVIEKLVSDHCIVCKYT 406

BLAST of Carg01101 vs. NCBI nr
Match: XP_022971396.1 (lactosylceramide 4-alpha-galactosyltransferase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 803.5 bits (2074), Expect = 8.3e-229
Identity = 389/400 (97.25%), Postives = 396/400 (99.00%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60
           MSERCTVSPILPTILLAAIIIF+CGDG+IYKASIRTVSINENKNSNSTITC+TQEPLKSS
Sbjct: 1   MSERCTVSPILPTILLAAIIIFICGDGMIYKASIRTVSINENKNSNSTITCITQEPLKSS 60

Query: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120
           PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS
Sbjct: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120

Query: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180
           HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLD 
Sbjct: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDR 180

Query: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240
           GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI
Sbjct: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240

Query: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300
           DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS
Sbjct: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300

Query: 301 RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA 360
           RWGYNGPFLVSRVIAK+TGAG RPGFNVTIL PAAFYPVDWTKIGELFKKPGNRASANWA
Sbjct: 301 RWGYNGPFLVSRVIAKVTGAGDRPGFNVTILPPAAFYPVDWTKIGELFKKPGNRASANWA 360

Query: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHC 401
           KAML+QLND+AYGIHLWNKQSKSYTIQKGSVIE+LVS HC
Sbjct: 361 KAMLSQLNDKAYGIHLWNKQSKSYTIQKGSVIEKLVSDHC 400

BLAST of Carg01101 vs. ExPASy Swiss-Prot
Match: Q67BJ4 (Lactosylceramide 4-alpha-galactosyltransferase OS=Mus musculus OX=10090 GN=A4galt PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.5e-31
Identity = 88/273 (32.23%), Postives = 146/273 (53.48%), Query Frame = 0

Query: 143 LLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDHGFKILAITP-------DLPSLF 202
           + + ES  ++HP   + +L + L  +      QP  + G  +L+  P       DL  LF
Sbjct: 101 MCSVESAARAHPESQVVVLMKGLPRD---TTAQP-RNLGISLLSCFPNVWIRPLDLQELF 160

Query: 203 KNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYIDTDFIVLKSFMGLKN 262
           ++TP+  W+ E  R + +P Q+P+   LS+  R+A+L+K+GG+Y+DTDFIVLK+ + L N
Sbjct: 161 EDTPLAAWYSE-ARHRWEPYQLPV---LSDASRIALLWKFGGIYLDTDFIVLKNLLNLTN 220

Query: 263 CIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGSRWGYNGPFLVSRVIA 322
            +G QS          LN A L F++KH  L   + +F +N++G  WG+ GP L++RV  
Sbjct: 221 TLGIQS-------RYVLNGAFLAFERKHEFLALCLHDFVANYNGWIWGHQGPQLLTRVFK 280

Query: 323 K------ITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWAKAMLAQLND 382
           K      +  + A  G  VT L P AFYP+ W    + F+        + +   L QL +
Sbjct: 281 KWCSIQSLEKSHACRG--VTALPPEAFYPIPWQNWKKYFE--------DISPEELTQLLN 340

Query: 383 EAYGIHLWNKQSKSYTIQKGS--VIERLVSAHC 401
             Y +H+WNK+S+   ++  S  ++ +L + +C
Sbjct: 341 ATYAVHVWNKKSQGTHLEATSKALLAQLHARYC 348

BLAST of Carg01101 vs. ExPASy Swiss-Prot
Match: Q9JI93 (Lactosylceramide 4-alpha-galactosyltransferase OS=Rattus norvegicus OX=10116 GN=A4galt PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.7e-30
Identity = 86/267 (32.21%), Postives = 143/267 (53.56%), Query Frame = 0

Query: 143 LLAAESIFKSHPRGCLTILSRTL--DSERGYKILQPLLDHGFKILAITP-DLPSLFKNTP 202
           + + ES  ++HP   + +L + L  D+    + L   L   F  + I P DL  LF++TP
Sbjct: 102 MCSVESAARAHPESQVVVLMKGLPRDTTAWPRNLGISLLSCFPNVQIRPLDLQELFEDTP 161

Query: 203 VETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYIDTDFIVLKSFMGLKNCIGA 262
           +  W+ E +  + +P  +P+   LS+  R+A+L+K+GG+Y+DTDFIVLK+   L N +G 
Sbjct: 162 LAAWYLEAQH-RWEPYLLPV---LSDASRIALLWKFGGIYLDTDFIVLKNLRNLTNMLGI 221

Query: 263 QSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGSRWGYNGPFLVSRVIAKITG 322
           QS          LN A L F++KH  L   + +F ++++G  WG+ GP L++RV  K   
Sbjct: 222 QS-------RYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQLLTRVFKKWCS 281

Query: 323 ----AGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWAKAMLAQLNDEAYGIH 382
                 +R    VT L P AFYP+ W    + F+        + +   LAQL +  Y +H
Sbjct: 282 IHSLKESRACRGVTALPPEAFYPIPWQNWKKYFE--------DVSPEELAQLLNATYAVH 341

Query: 383 LWNKQSKSYTIQKGS--VIERLVSAHC 401
           +WNK+S+   ++  S  ++ +L + +C
Sbjct: 342 VWNKKSQGTHLEATSRALLAQLHARYC 349

BLAST of Carg01101 vs. ExPASy Swiss-Prot
Match: Q9NPC4 (Lactosylceramide 4-alpha-galactosyltransferase OS=Homo sapiens OX=9606 GN=A4GALT PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.0e-28
Identity = 83/271 (30.63%), Postives = 138/271 (50.92%), Query Frame = 0

Query: 143 LLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDHGFKILAITP-------DLPSLF 202
           + + ES  ++HP   + +L + L    G   L   L  G  +L+  P       DL  LF
Sbjct: 95  MCSVESAARTHPESHVLVLMKGLPG--GNASLPRHL--GISLLSCFPNVQMLPLDLRELF 154

Query: 203 KNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYIDTDFIVLKSFMGLKN 262
           ++TP+  W+  + +G+ +P  +P+   LS+  R+A+++K+GG+Y+DTDFIVLK+   L N
Sbjct: 155 RDTPLADWYAAV-QGRWEPYLLPV---LSDASRIALMWKFGGIYLDTDFIVLKNLRNLTN 214

Query: 263 CIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGSRWGYNGPFLVSRVIA 322
            +G QS          LN A L F+++H  +   M +F  +++G  WG+ GP L++RV  
Sbjct: 215 VLGTQS-------RYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFK 274

Query: 323 KITG----AGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWAKAMLAQLNDEA 382
           K       A +R    VT L P AFYP+ W    + F+              L +L    
Sbjct: 275 KWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEE--------LPRLLSAT 334

Query: 383 YGIHLWNKQSKSYTIQKGS--VIERLVSAHC 401
           Y +H+WNK+S+    +  S  ++ +L + +C
Sbjct: 335 YAVHVWNKKSQGTRFEATSRALLAQLHARYC 342

BLAST of Carg01101 vs. ExPASy Swiss-Prot
Match: Q9N291 (Lactosylceramide 4-alpha-galactosyltransferase OS=Pan troglodytes OX=9598 GN=A4GALT PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.7e-28
Identity = 83/271 (30.63%), Postives = 138/271 (50.92%), Query Frame = 0

Query: 143 LLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDHGFKILAITP-------DLPSLF 202
           + + ES  ++HP   + +L + L    G   L   L  G  +L+  P       DL  LF
Sbjct: 95  MCSVESAARTHPESHVLVLMKGLPG--GNASLPRHL--GISLLSCFPNVQMLPLDLRELF 154

Query: 203 KNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYIDTDFIVLKSFMGLKN 262
           ++TP+  W+  + +G+ +P  +P+   LS+  R+A+++K+GG+Y+DTDFIVLK+   L N
Sbjct: 155 QDTPLADWYAAV-QGRWEPYLLPV---LSDASRIALMWKFGGIYLDTDFIVLKNLRNLTN 214

Query: 263 CIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGSRWGYNGPFLVSRVIA 322
            +G QS          LN A L F+++H  +   M +F  +++G  WG+ GP L++RV  
Sbjct: 215 VLGTQS-------RYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFK 274

Query: 323 KITG----AGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWAKAMLAQLNDEA 382
           K       A +R    VT L P AFYP+ W    + F+              L +L    
Sbjct: 275 KWCSIRSLAESRSCRGVTTLPPEAFYPIPWQDWKKYFEDINPEE--------LPRLLSAT 334

Query: 383 YGIHLWNKQSKSYTIQKGS--VIERLVSAHC 401
           Y +H+WNK+S+    +  S  ++ +L + +C
Sbjct: 335 YAVHVWNKKSQGTRFEATSRALLAQLHARYC 342

BLAST of Carg01101 vs. ExPASy Swiss-Prot
Match: Q9N290 (Lactosylceramide 4-alpha-galactosyltransferase (Fragment) OS=Gorilla gorilla gorilla OX=9595 GN=A4GALT PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 5.9e-28
Identity = 83/271 (30.63%), Postives = 137/271 (50.55%), Query Frame = 0

Query: 143 LLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDHGFKILAITP-------DLPSLF 202
           + + ES  ++HP   + +L + L    G   L   L  G  +L+  P       DL  LF
Sbjct: 69  MCSVESAARTHPESHVLVLMKGLPG--GNASLPRHL--GISLLSCFPNVQMLPLDLRELF 128

Query: 203 KNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYIDTDFIVLKSFMGLKN 262
           ++TP+  W+  + +G+ +P  +P+   LS+  R+A+++K+GG+Y+DTDFIVLK+   L N
Sbjct: 129 RDTPLADWYAAV-QGRWEPYLLPV---LSDASRIALMWKFGGIYLDTDFIVLKNLRNLTN 188

Query: 263 CIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGSRWGYNGPFLVSRVIA 322
            +G QS          LN A L F++ H  +   M +F  +++G  WG+ GP L++RV  
Sbjct: 189 VLGTQS-------RYVLNGAFLAFERXHEFMALCMXDFVDHYNGWIWGHQGPQLLTRVFK 248

Query: 323 KITG----AGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWAKAMLAQLNDEA 382
           K       A +R    VT L P AFYP+ W    + F+              L +L    
Sbjct: 249 KWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEE--------LPRLFSAT 308

Query: 383 YGIHLWNKQSKSYTIQKGS--VIERLVSAHC 401
           Y +H+WNK+S+    +  S  ++ +L + +C
Sbjct: 309 YAVHVWNKKSQGTRFEATSRALLAQLHARYC 316

BLAST of Carg01101 vs. ExPASy TrEMBL
Match: A0A6J1GA34 (lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111452297 PE=4 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.1e-237
Identity = 404/406 (99.51%), Postives = 405/406 (99.75%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60
           MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS
Sbjct: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60

Query: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120
           PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS
Sbjct: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120

Query: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180
           HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH
Sbjct: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180

Query: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240
           GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI
Sbjct: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240

Query: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300
           DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS
Sbjct: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300

Query: 301 RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA 360
           RWGYNGPFLVSRVIAK+TGAGARPGFNVTIL PAAFYPVDWTKIGELFKKPGNRASANWA
Sbjct: 301 RWGYNGPFLVSRVIAKVTGAGARPGFNVTILPPAAFYPVDWTKIGELFKKPGNRASANWA 360

Query: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 407
           KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT
Sbjct: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 406

BLAST of Carg01101 vs. ExPASy TrEMBL
Match: A0A6J1I1U4 (lactosylceramide 4-alpha-galactosyltransferase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470132 PE=4 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 6.0e-233
Identity = 394/406 (97.04%), Postives = 402/406 (99.01%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60
           MSERCTVSPILPTILLAAIIIF+CGDG+IYKASIRTVSINENKNSNSTITC+TQEPLKSS
Sbjct: 1   MSERCTVSPILPTILLAAIIIFICGDGMIYKASIRTVSINENKNSNSTITCITQEPLKSS 60

Query: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120
           PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS
Sbjct: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120

Query: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180
           HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLD 
Sbjct: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDR 180

Query: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240
           GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI
Sbjct: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240

Query: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300
           DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS
Sbjct: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300

Query: 301 RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA 360
           RWGYNGPFLVSRVIAK+TGAG RPGFNVTIL PAAFYPVDWTKIGELFKKPGNRASANWA
Sbjct: 301 RWGYNGPFLVSRVIAKVTGAGDRPGFNVTILPPAAFYPVDWTKIGELFKKPGNRASANWA 360

Query: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 407
           KAML+QLND+AYGIHLWNKQSKSYTIQKGSVIE+LVS HCI+CKYT
Sbjct: 361 KAMLSQLNDKAYGIHLWNKQSKSYTIQKGSVIEKLVSDHCIVCKYT 406

BLAST of Carg01101 vs. ExPASy TrEMBL
Match: A0A6J1I375 (lactosylceramide 4-alpha-galactosyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470132 PE=4 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.0e-229
Identity = 389/400 (97.25%), Postives = 396/400 (99.00%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSS 60
           MSERCTVSPILPTILLAAIIIF+CGDG+IYKASIRTVSINENKNSNSTITC+TQEPLKSS
Sbjct: 1   MSERCTVSPILPTILLAAIIIFICGDGMIYKASIRTVSINENKNSNSTITCITQEPLKSS 60

Query: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120
           PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS
Sbjct: 61  PENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLS 120

Query: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDH 180
           HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLD 
Sbjct: 121 HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDR 180

Query: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240
           GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI
Sbjct: 181 GFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYI 240

Query: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300
           DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS
Sbjct: 241 DTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGS 300

Query: 301 RWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWA 360
           RWGYNGPFLVSRVIAK+TGAG RPGFNVTIL PAAFYPVDWTKIGELFKKPGNRASANWA
Sbjct: 301 RWGYNGPFLVSRVIAKVTGAGDRPGFNVTILPPAAFYPVDWTKIGELFKKPGNRASANWA 360

Query: 361 KAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHC 401
           KAML+QLND+AYGIHLWNKQSKSYTIQKGSVIE+LVS HC
Sbjct: 361 KAMLSQLNDKAYGIHLWNKQSKSYTIQKGSVIEKLVSDHC 400

BLAST of Carg01101 vs. ExPASy TrEMBL
Match: A0A5A7TRE8 (Lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00900 PE=4 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.5e-199
Identity = 342/415 (82.41%), Postives = 368/415 (88.67%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQ------ 60
           MSERC +SPILPTILLAAII  +CGDGIIYK SIRTVS++E +NSNSTITC+TQ      
Sbjct: 1   MSERCAISPILPTILLAAIIFLICGDGIIYKVSIRTVSLDEKRNSNSTITCITQLQIKQT 60

Query: 61  ---EPLKSSPENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQF 120
              EP +SSPENR+E DDS TFDSL+VP NLS K AR+DWFR HLP+FRILQSNN TQQF
Sbjct: 61  LVGEPRQSSPENRDE-DDSETFDSLVVPSNLSAKDARVDWFRNHLPNFRILQSNNLTQQF 120

Query: 121 HNRLQEFLSHDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGY 180
           HNRL EFLSH+CE QFFMTW+SP R+FR REL+AAES+FKSHPRGCLTI+SRTLDSERG 
Sbjct: 121 HNRLLEFLSHECEVQFFMTWVSPARSFRERELMAAESVFKSHPRGCLTIISRTLDSERGC 180

Query: 181 KILQPLLDHGFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAV 240
           KIL+PLLDHGFKI AI PDLP LF+NTPVE WFDEMK GKKDPGQIPLAQNLSNLMRLAV
Sbjct: 181 KILKPLLDHGFKIQAIAPDLPLLFRNTPVEAWFDEMKSGKKDPGQIPLAQNLSNLMRLAV 240

Query: 241 LYKYGGVYIDTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFME 300
           LYKYGGVYIDTDFIVLKSFMGLKN IGAQSIDPV+KNWT LNNAVLVFDKKHPLLEKFME
Sbjct: 241 LYKYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPVTKNWTILNNAVLVFDKKHPLLEKFME 300

Query: 301 NFASNFDGSRWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKP 360
           NFASNFDGSRWGYNGPFLV+RVIAK+TGA A PGFNVT+L PAAFYPVDW KIGELFKKP
Sbjct: 301 NFASNFDGSRWGYNGPFLVTRVIAKVTGARASPGFNVTVLPPAAFYPVDWIKIGELFKKP 360

Query: 361 GNRASANWAKAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 407
            NRA  +WAKA LAQL++E YGIHLWNKQSKSY IQKGSVIERL S HCIIC YT
Sbjct: 361 ENRAVESWAKARLAQLDNETYGIHLWNKQSKSYVIQKGSVIERLFSDHCIICSYT 414

BLAST of Carg01101 vs. ExPASy TrEMBL
Match: A0A1S4DUL6 (lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo OX=3656 GN=LOC103486309 PE=4 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.5e-199
Identity = 342/415 (82.41%), Postives = 368/415 (88.67%), Query Frame = 0

Query: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQ------ 60
           MSERC +SPILPTILLAAII  +CGDGIIYK SIRTVS++E +NSNSTITC+TQ      
Sbjct: 1   MSERCAISPILPTILLAAIIFLICGDGIIYKVSIRTVSLDEKRNSNSTITCITQLQIKQT 60

Query: 61  ---EPLKSSPENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQF 120
              EP +SSPENR+E DDS TFDSL+VP NLS K AR+DWFR HLP+FRILQSNN TQQF
Sbjct: 61  LVGEPRQSSPENRDE-DDSETFDSLVVPSNLSAKDARVDWFRNHLPNFRILQSNNLTQQF 120

Query: 121 HNRLQEFLSHDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGY 180
           HNRL EFLSH+CE QFFMTW+SP R+FR REL+AAES+FKSHPRGCLTI+SRTLDSERG 
Sbjct: 121 HNRLLEFLSHECEVQFFMTWVSPARSFRERELMAAESVFKSHPRGCLTIISRTLDSERGC 180

Query: 181 KILQPLLDHGFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAV 240
           KIL+PLLDHGFKI AI PDLP LF+NTPVE WFDEMK GKKDPGQIPLAQNLSNLMRLAV
Sbjct: 181 KILKPLLDHGFKIQAIAPDLPLLFRNTPVEAWFDEMKSGKKDPGQIPLAQNLSNLMRLAV 240

Query: 241 LYKYGGVYIDTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFME 300
           LYKYGGVYIDTDFIVLKSFMGLKN IGAQSIDPV+KNWT LNNAVLVFDKKHPLLEKFME
Sbjct: 241 LYKYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPVTKNWTILNNAVLVFDKKHPLLEKFME 300

Query: 301 NFASNFDGSRWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKP 360
           NFASNFDGSRWGYNGPFLV+RVIAK+TGA A PGFNVT+L PAAFYPVDW KIGELFKKP
Sbjct: 301 NFASNFDGSRWGYNGPFLVTRVIAKVTGARASPGFNVTVLPPAAFYPVDWIKIGELFKKP 360

Query: 361 GNRASANWAKAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 407
            NRA  +WAKA LAQL++E YGIHLWNKQSKSY IQKGSVIERL S HCIIC YT
Sbjct: 361 ENRAVESWAKARLAQLDNETYGIHLWNKQSKSYVIQKGSVIERLFSDHCIICSYT 414

BLAST of Carg01101 vs. TAIR 10
Match: AT5G01250.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 384.8 bits (987), Expect = 8.6e-107
Identity = 175/304 (57.57%), Postives = 219/304 (72.04%), Query Frame = 0

Query: 101 ILQSNNFTQQFHNRLQEFLSHDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTI 160
           +   +N + +F  R+ EF+   CE  F MTWISP   F  RE+LA ES+FKSHP GCL I
Sbjct: 101 VFSGDNLSDKFQKRVNEFVGDGCEVNFVMTWISPADFFGNREVLAIESVFKSHPYGCLMI 160

Query: 161 LSRTLDSERGYKILQPLLDHGFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLA 220
           LS T+DS +GY  L+P +D G+K+LA+TPDLP L K T  E W DE+K GK+DPG+I LA
Sbjct: 161 LSATMDSPQGYATLKPFIDRGYKVLAVTPDLPFLLKGTAGELWLDEIKSGKRDPGKISLA 220

Query: 221 QNLSNLMRLAVLYKYGGVYIDTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFD 280
           QNLSNLMRLA LYKYGGVY+DTD IVLKSF GL+N IGAQ++DP S NWTRLNNAVL+FD
Sbjct: 221 QNLSNLMRLAYLYKYGGVYLDTDMIVLKSFKGLRNVIGAQTLDPSSTNWTRLNNAVLIFD 280

Query: 281 KKHPLLEKFMENFASNFDGSRWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVD 340
           K HPLL KFME FA  F+G+ WGYNGP+LVSRV   + G+    G+N T++ P+ FY V+
Sbjct: 281 KNHPLLLKFMEEFAKTFNGNIWGYNGPYLVSRVARAVEGSS---GYNFTVMRPSVFYSVN 340

Query: 341 WTKIGELFKKPGNRASANWAKAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHC 400
           W +I +LFK P     + W K  L  +    YG+HLWNK S+ Y I++GS + +LVS HC
Sbjct: 341 WLEIKKLFKVPKTEKDSKWVKTKLLHMQRNGYGLHLWNKFSRKYEIEQGSAMWKLVSEHC 400

Query: 401 IICK 405
           IIC+
Sbjct: 401 IICE 401

BLAST of Carg01101 vs. TAIR 10
Match: AT2G38150.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 379.0 bits (972), Expect = 4.7e-105
Identity = 170/328 (51.83%), Postives = 228/328 (69.51%), Query Frame = 0

Query: 77  IVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLSHDCETQFFMTWISPTR 136
           ++P   + K  RIDWFR+ LP+  IL+S   ++ FH R+ +  + +C  QFFM W+SP  
Sbjct: 73  LLPPRKASKNQRIDWFRRKLPELEILKSTTKSKSFHTRVLDLYNKNCSAQFFMIWLSPAN 132

Query: 137 TFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDHGFKILAITPDLPSLFK 196
           +F  RE+LA +++F ++P  CL ILS +LDS  GY IL+PL D GF ++A+T D+P L K
Sbjct: 133 SFGPREMLAIDTLFTTNPGACLAILSNSLDSPNGYTILKPLFDQGFNLIAVTIDIPFLVK 192

Query: 197 NTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYIDTDFIVLKSFMGLKNC 256
           NTP E W   +K G  DPG IPL  NLS+L RLAVLYKYGGVY+DTD I L    GL+N 
Sbjct: 193 NTPAEAWLKRLKSGNMDPGSIPLFMNLSDLTRLAVLYKYGGVYLDTDIIFLNDMTGLRNA 252

Query: 257 IGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGSRWGYNGPFLVSRVIAK 316
           IGAQS DP +K WTRLNNAV+VFD  HPL+ +F++ +A+ FDG++WGYN P+LVSRVI +
Sbjct: 253 IGAQSSDPATKRWTRLNNAVMVFDIYHPLMREFLQEYATTFDGNKWGYNSPYLVSRVIKR 312

Query: 317 ITGAGARPGF-NVTILSPAAFYPVDWTKIGELFKKPGNRASANWAKAMLAQLNDEAYGIH 376
           +   G +PG+ N+TI SP AFYPV+W KI +LFKKP     A W +  +  +N  +Y IH
Sbjct: 313 L---GNKPGYNNLTIFSPDAFYPVNWIKIQKLFKKPATTREAKWVEKTVQDMNKGSYMIH 372

Query: 377 LWNKQSKSYTIQKGSVIERLVSAHCIIC 404
           LWNK ++   I++GSV+  LVS HC +C
Sbjct: 373 LWNKVTRKIKIEEGSVMHTLVSTHCTVC 397

BLAST of Carg01101 vs. TAIR 10
Match: AT3G09020.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 366.3 bits (939), Expect = 3.2e-101
Identity = 164/303 (54.13%), Postives = 225/303 (74.26%), Query Frame = 0

Query: 101 ILQSNNFTQQFHNRLQEFLSHDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTI 160
           +    + +++F  R  EFL  DCE +F MTWISP   F +RE+L+ ES+FKSH RGCL I
Sbjct: 105 VFGGKDVSEKFQQRATEFLRDDCEVKFMMTWISPAELFGKREILSVESVFKSHARGCLMI 164

Query: 161 LSRTLDSERGYKILQPLLDHGFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLA 220
           LS T+DS +G++IL+P LD G++++A+TPDLP L K+T  E+W +E++ GK+DPG+I LA
Sbjct: 165 LSSTMDSLQGFRILKPFLDRGYRVMAVTPDLPFLLKDTAGESWLEEIQTGKRDPGKISLA 224

Query: 221 QNLSNLMRLAVLYKYGGVYIDTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFD 280
           QNLSNLMRLA L+K+GGVY+DTD IVLKSF  L+N IGAQ+++PVS+NWTRLNNAVL+FD
Sbjct: 225 QNLSNLMRLAYLFKFGGVYLDTDMIVLKSFKTLRNVIGAQTLEPVSRNWTRLNNAVLIFD 284

Query: 281 KKHPLLEKFMENFASNFDGSRWGYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVD 340
           K HP L K +E FA  F+G+ WG+NGP+LVSRV   + G     G+N TIL+P AFYPV+
Sbjct: 285 KNHPFLLKSIEEFALTFNGNVWGHNGPYLVSRVARAVEGT---DGYNFTILTPPAFYPVN 344

Query: 341 WTKIGELFKKPGNRASANWAKAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHC 400
           W +I +LFK P     +   +  + ++   +YG+HLWNK S+ + I++GS +++LVS  C
Sbjct: 345 WVEIEKLFKVPRTEKDSKRVQVKVLEMQKRSYGLHLWNKFSRKFEIEQGSAMDKLVSNQC 404

Query: 401 IIC 404
           IIC
Sbjct: 405 IIC 404

BLAST of Carg01101 vs. TAIR 10
Match: AT2G38152.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 345.5 bits (885), Expect = 5.8e-95
Identity = 182/402 (45.27%), Postives = 247/402 (61.44%), Query Frame = 0

Query: 4   RCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQEPLKSSPEN 63
           R T SP+  T + A I++ +    I+   S++         S    +  T E ++++   
Sbjct: 21  RHTNSPVFSTAIFAVIMLLVVAGTILSNMSLK---------STFFWSSPTSEVIQTNRME 80

Query: 64  REEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQFHNRLQEFLSHDC 123
           R+         SL  P N + +  RI W   HL +F                        
Sbjct: 81  RK---------SLAPPKNTTSRD-RIAWLHSHLTEF------------------------ 140

Query: 124 ETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGYKILQPLLDHGFK 183
           E +FFMTW SP   F +RE+LA ES+FK+HP+GCL I+S +LDS +G  IL+PL D G+K
Sbjct: 141 EVRFFMTWFSPAEYFGKREMLAVESVFKAHPQGCLMIVSGSLDSLQGDSILKPLNDRGYK 200

Query: 184 ILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAVLYKYGGVYIDTD 243
           + A TPD+  L +NTP ++WF EMK  K+DPG+IPL QNLSNL RLA LYKYGGVY+DTD
Sbjct: 201 VFAATPDMSLLLENTPAKSWFQEMKSCKRDPGRIPLHQNLSNLARLAFLYKYGGVYLDTD 260

Query: 244 FIVLKSFMGLKNCIGAQS-IDPVSKNWTRLNNAVLVFDKKHPLLEKFMENFASNFDGSRW 303
           FIV +SF GLKN IGAQ+ ++  SKNWTRLNNAVL+F+K HPL+  F+E FAS FDG++W
Sbjct: 261 FIVTRSFKGLKNSIGAQTVVEGDSKNWTRLNNAVLIFEKDHPLVYSFIEEFASTFDGNKW 320

Query: 304 GYNGPFLVSRVIAKITGAGARPGFNVTILSPAAFYPVDWTKIGELFKKPGNRASANWAKA 363
           G+NGP+LV+RV  +   A    G N T+L P AFYP +W  I  LF+ P     +   K 
Sbjct: 321 GHNGPYLVTRVAQR---ARETIGDNFTVLPPVAFYPFNWLDIPRLFQTPRGSNDSTLLKT 376

Query: 364 MLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICK 405
            L +LN E+YG+HLWNK ++   I KGSVI+ ++S HC++C+
Sbjct: 381 DLVKLNRESYGLHLWNKITRKLKIGKGSVIDIIISDHCVVCR 376

BLAST of Carg01101 vs. TAIR 10
Match: AT1G61050.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 329.3 bits (843), Expect = 4.3e-90
Identity = 151/297 (50.84%), Postives = 207/297 (69.70%), Query Frame = 0

Query: 109 QQFHNRLQEFLS-HDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDS 168
           Q+F  R++  LS   CE+ FFMTWIS   +F  RE    ES+FK HP GCL ++S + D 
Sbjct: 134 QRFQTRVKSLLSKSSCESLFFMTWISSIESFGDRERFTIESLFKFHPNGCLILVSNSFDC 193

Query: 169 ERGYKILQPLLDHGFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLM 228
           +RG  IL+P  D G K+L I PD   +FK+T  E WF+ +K+G   PG IPL QNLSNL+
Sbjct: 194 DRGTLILKPFTDKGLKVLPIKPDFAYIFKDTSAEKWFERLKKGTLSPGVIPLEQNLSNLL 253

Query: 229 RLAVLYKYGGVYIDTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLE 288
           RL +LYKYGG+Y+DTD I+LKS   L N IGAQ++DPV+K W+RLNNAVL+FDK HPLL+
Sbjct: 254 RLVLLYKYGGIYLDTDVIILKSLSNLHNVIGAQTVDPVTKKWSRLNNAVLIFDKNHPLLK 313

Query: 289 KFMENFASNFDGSRWGYNGPFLVSRVIAKI-TGAGARPGFNVTILSPAAFYPVDWTKIGE 348
           +F++ F+  F+G++WG+NGP+LVSRVI +I   + +  GF+V  L P+AFYPVDWT+I  
Sbjct: 314 RFIDEFSRTFNGNKWGHNGPYLVSRVITRIKISSSSDLGFSV--LPPSAFYPVDWTRIKG 373

Query: 349 LFKKPGNRASANWAKAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIIC 404
            ++ P N + A W +  L  L    + +HLWN++SK   I++GS+I +L+S  CI C
Sbjct: 374 FYRAPTNESDA-WLRKRLTHLRKNTFAVHLWNRESKKLRIEEGSIIHQLMSHSCIFC 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6596350.11.3e-237100.00Lactosylceramide 4-alpha-galactosyltransferase, partial [Cucurbita argyrosperma ... [more]
XP_022948708.16.3e-23799.51lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita moschata][more]
XP_023539712.11.2e-23598.77lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita pepo subsp. pepo][more]
XP_022971397.11.2e-23297.04lactosylceramide 4-alpha-galactosyltransferase-like isoform X2 [Cucurbita maxima... [more]
XP_022971396.18.3e-22997.25lactosylceramide 4-alpha-galactosyltransferase-like isoform X1 [Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
Q67BJ41.5e-3132.23Lactosylceramide 4-alpha-galactosyltransferase OS=Mus musculus OX=10090 GN=A4gal... [more]
Q9JI933.7e-3032.21Lactosylceramide 4-alpha-galactosyltransferase OS=Rattus norvegicus OX=10116 GN=... [more]
Q9NPC42.0e-2830.63Lactosylceramide 4-alpha-galactosyltransferase OS=Homo sapiens OX=9606 GN=A4GALT... [more]
Q9N2912.7e-2830.63Lactosylceramide 4-alpha-galactosyltransferase OS=Pan troglodytes OX=9598 GN=A4G... [more]
Q9N2905.9e-2830.63Lactosylceramide 4-alpha-galactosyltransferase (Fragment) OS=Gorilla gorilla gor... [more]
Match NameE-valueIdentityDescription
A0A6J1GA343.1e-23799.51lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita moschata OX=366... [more]
A0A6J1I1U46.0e-23397.04lactosylceramide 4-alpha-galactosyltransferase-like isoform X2 OS=Cucurbita maxi... [more]
A0A6J1I3754.0e-22997.25lactosylceramide 4-alpha-galactosyltransferase-like isoform X1 OS=Cucurbita maxi... [more]
A0A5A7TRE81.5e-19982.41Lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S4DUL61.5e-19982.41lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo OX=3656 GN=LOC103... [more]
Match NameE-valueIdentityDescription
AT5G01250.18.6e-10757.57alpha 1,4-glycosyltransferase family protein [more]
AT2G38150.14.7e-10551.83alpha 1,4-glycosyltransferase family protein [more]
AT3G09020.13.2e-10154.13alpha 1,4-glycosyltransferase family protein [more]
AT2G38152.15.8e-9545.27alpha 1,4-glycosyltransferase family protein [more]
AT1G61050.14.3e-9050.84alpha 1,4-glycosyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007577Glycosyltransferase, DXD sugar-binding motifPFAMPF04488Gly_transf_sugcoord: 141..260
e-value: 5.8E-23
score: 81.4
IPR007652Alpha 1,4-glycosyltransferase domainPFAMPF04572Gb3_synthcoord: 279..403
e-value: 1.0E-28
score: 100.1
NoneNo IPR availableGENE3D3.90.550.20coord: 19..327
e-value: 1.5E-23
score: 85.7
NoneNo IPR availablePANTHERPTHR46781:SF2ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEINcoord: 40..405
IPR044789Putative alpha 1,4-glycosyltransferase, plantPANTHERPTHR46781ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEINcoord: 40..405
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 88..394

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01101-RACarg01101-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016757 glycosyltransferase activity