Carg01091 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01091
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 8.1-like
LocationCarg_Chr06: 1269389 .. 1271960 (+)
RNA-Seq ExpressionCarg01091
SyntenyCarg01091
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGGTTTTGCAGTTTCATTATGTGATTAAATGTTGAGTGTTTGGCTGTGACGAACAAGAAGCGGGCTGCTCTCTCTGTCATTGTTTTTCTCGCGCCTTCATCTTCTTCAACTCATCGCTCTGATCCCTTTGTTTGCTCTTGTTCTTCAAGGTTTTTGATTCTACACTTTCATAAACTTCAACCCTTTTCATTATTTTCGATCATCTCAAGAGGGTTCGCTTTGGATCTTCCAGAAATCACTTGATTCTGTTTGCTTTGTGATTGATTTTATATATTGATTTTGAGTTCTTCAAGCATGTGATACGATTCAGTTGTTCTCTGTTTTATTTGTGTTCTTTGATTGCGACACTCAAACCTCGGAGTGATTAAAAATTTAGTGCGTTGAATTTTAAATTCATTCGTGGAAAAGGGCAAGTTCATCCGTACCCTTTTATTATTTTAACCAAACTCAAACAATCAGAGTTGAAAAGTTGAATGTGGTTTTGGATTGTTCTTGCCCTCGAGATTATTAGGTTCTAATTTTACTGATTGGGGTTTAGTTTTATAATCAACAGGCTCAGTAGGTATTAGCATATCAAGAGGAAGTTTTCAATGGCAGAAGATGATGTGTATACAAAAGATGGTACCATTGATATTCATAAGAATCCTGCTAATAAGAAGAAGACTGGAAAATGGAAGGCTTGTCGCTTTATTCTTGGTATGTTTACTCTGTTCTTAACGTTTGATTTTGATTGTTCTTTGCTGAAATTTGTAACGATGATGTTTGTAAAGGGAATGAATGCTGTGAAAGATTGGCATATTATGGGATGAGTACCAATCTAGTGAACTATCTTCAAATACGTCTTAACATGGACAATGTAGCTGCTTCAAATAGTGTTACTAGTTGGTCAGGAACCTGCTACCTTACACCATTGATTGGAGCTTTCTTGGCTGATGCTTACTTGGGAAGATATTGGACTATTGCTAGTTTCTCAATCTTCTATGTCTTTGTAAGTTTTTCTTGTCGATCGGGCGAAAAGAGAACGAGTTGTTCTTCAAATCACATAAGAACTTGAACGCATTGTAGTGATACCATGTTTATGATATATGGTTTTGCAGGGCATGACATTGTTGACGATGGCTGCTTCAGTCCCTGGATTGAAGCCATCTTGTGACAATACTGTTTGCCATCCAACTGGAGGTCAAACGGCTGTTACCTTCATAGCACTTTACTTGATCGCCCTCGGGACCGGGGGAATCAAGCCATGTGTGTCGTCGTTTGGGGCCGATCAATTCGACGAAAATGATGAAGGCGAAAGGAAGAAGAAAAGCTCCTTCTTCAATTGGTTTTACTTTTCAATCAACGTTGGTGCAATGATTGCCTCCTCAGTTCTAGTATGGATACAAATGAATGTAGGGTGGGGGTGGGGCTTTGGAGTTCCTGCAGTAGCCATGGCCATTGCTGTAGTGTTTTTCTTCTCCGGTAGCTCGTTGTATCGTCTGCAAAAGCCAGCAGGAAGTCCTCTCACAAGAATCTTGCAAGTTATAGTTGCAGCTTGTAGGAAACAAGGAGTACAAGTTCCAGAGGATAAGTCTCTCCTTTACGAGACTGCTGATGATGTCGAGTCGAAAATCGAAGGAAGTCGCAAGCTCGAGCACACGAACGAGTTAAAGTAAGTATATTACTGGGATCAGAATCGAAGGAAGTCGAAAATCATGGAGAGATTCTTATATTATCCAAATTGGTTACATTTTTTCAGGTTCCTAGATAAGGCTTGTGTATTGAGGGAAAGTGATGGAATGAAGGGGCAAGGGAATGCATGGATACTTTGTACAGTGACACAAGTTGAGGAGCTGAAGAGCATTATCAGGTTGTTGCCTGTGTGGGCGTGTGGAATAGTATTTTCAGCGGTGTATAGCCAAATGAGCACCATGTTTGTTCTACAAGGTAATATAATGGACCAGCACATTGGGGCAAGTTTCAAAATTCCATCGGCCTCGCTTTCGATCTTCGATACCCTCAGCGTTCTCTTTTGGGCTCCCGTATACGACAGACTCATCGTCCCAATGGCGAAGAAGTTCACGAACAATGAGCGTGGCTTCACACAGCTACAACGGATGGGAATAGGACTTGTCATATCTGTTTTCTCCATGGTTGCAGCAGGTGTGTTGGAAGTTTTCAGGCTGGAGTATGTGAGGAAGAACAAGCTATACGATGCGGAGTACATTCCGATGTCGATATTCTGGCAAGTTCCACAGTATTTTTTGATCGGATGTGCAGAAGTGTTCACTTTTATAGGACAGTTAGAGTTTTTCTATGACCAAGCACCGGACGCAATGAGAAGCATGATGGCTGCCCTGTCGCTCACAACGGTTGGATTGGGAAACTACTTAAGCACATTGCTCGTTACAATCGTGACGGCCGTGACAACGAGACACGGGAAGCTCGGGTGGATTCCAAATAACTTAAACAAGGGACATCTCGACTATTTCTTCTGGCTGTTGGCCATTCTCAGCGTCCTCAACTTCTTTGCCTATCTCTTTGTGGCTAAGTGCTATCGCTACAAGAAGGTAACAGGTCATTTGCGCTGA

mRNA sequence

TTTGGTTTTGCAGTTTCATTATGTGATTAAATGTTGAGTGTTTGGCTGTGACGAACAAGAAGCGGGCTGCTCTCTCTGTCATTGTTTTTCTCGCGCCTTCATCTTCTTCAACTCATCGCTCTGATCCCTTTGTTTGCTCTTGTTCTTCAAGGCTCAGTAGGTATTAGCATATCAAGAGGAAGTTTTCAATGGCAGAAGATGATGTGTATACAAAAGATGGTACCATTGATATTCATAAGAATCCTGCTAATAAGAAGAAGACTGGAAAATGGAAGGCTTGTCGCTTTATTCTTGGGAATGAATGCTGTGAAAGATTGGCATATTATGGGATGAGTACCAATCTAGTGAACTATCTTCAAATACGTCTTAACATGGACAATGTAGCTGCTTCAAATAGTGTTACTAGTTGGTCAGGAACCTGCTACCTTACACCATTGATTGGAGCTTTCTTGGCTGATGCTTACTTGGGAAGATATTGGACTATTGCTAGTTTCTCAATCTTCTATGTCTTTGGCATGACATTGTTGACGATGGCTGCTTCAGTCCCTGGATTGAAGCCATCTTGTGACAATACTGTTTGCCATCCAACTGGAGGTCAAACGGCTGTTACCTTCATAGCACTTTACTTGATCGCCCTCGGGACCGGGGGAATCAAGCCATGTGTGTCGTCGTTTGGGGCCGATCAATTCGACGAAAATGATGAAGGCGAAAGGAAGAAGAAAAGCTCCTTCTTCAATTGGTTTTACTTTTCAATCAACGTTGGTGCAATGATTGCCTCCTCAGTTCTAGTATGGATACAAATGAATGTAGGGTGGGGGTGGGGCTTTGGAGTTCCTGCAGTAGCCATGGCCATTGCTGTAGTGTTTTTCTTCTCCGGTAGCTCGTTGTATCGTCTGCAAAAGCCAGCAGGAAGTCCTCTCACAAGAATCTTGCAAGTTATAGTTGCAGCTTGTAGGAAACAAGGAGTACAAGTTCCAGAGGATAAGTCTCTCCTTTACGAGACTGCTGATGATGTCGAGTCGAAAATCGAAGGAAGTCGCAAGCTCGAGCACACGAACGAGTTAAAGTTCCTAGATAAGGCTTGTGTATTGAGGGAAAGTGATGGAATGAAGGGGCAAGGGAATGCATGGATACTTTGTACAGTGACACAAGTTGAGGAGCTGAAGAGCATTATCAGGTTGTTGCCTGTGTGGGCGTGTGGAATAGTATTTTCAGCGGTGTATAGCCAAATGAGCACCATGTTTGTTCTACAAGGTAATATAATGGACCAGCACATTGGGGCAAGTTTCAAAATTCCATCGGCCTCGCTTTCGATCTTCGATACCCTCAGCGTTCTCTTTTGGGCTCCCGTATACGACAGACTCATCGTCCCAATGGCGAAGAAGTTCACGAACAATGAGCGTGGCTTCACACAGCTACAACGGATGGGAATAGGACTTGTCATATCTGTTTTCTCCATGGTTGCAGCAGGTGTGTTGGAAGTTTTCAGGCTGGAGTATGTGAGGAAGAACAAGCTATACGATGCGGAGTACATTCCGATGTCGATATTCTGGCAAGTTCCACAGTATTTTTTGATCGGATGTGCAGAAGTGTTCACTTTTATAGGACAGTTAGAGTTTTTCTATGACCAAGCACCGGACGCAATGAGAAGCATGATGGCTGCCCTGTCGCTCACAACGGTTGGATTGGGAAACTACTTAAGCACATTGCTCGTTACAATCGTGACGGCCGTGACAACGAGACACGGGAAGCTCGGGTGGATTCCAAATAACTTAAACAAGGGACATCTCGACTATTTCTTCTGGCTGTTGGCCATTCTCAGCGTCCTCAACTTCTTTGCCTATCTCTTTGTGGCTAAGTGCTATCGCTACAAGAAGGTAACAGGTCATTTGCGCTGA

Coding sequence (CDS)

ATGGCAGAAGATGATGTGTATACAAAAGATGGTACCATTGATATTCATAAGAATCCTGCTAATAAGAAGAAGACTGGAAAATGGAAGGCTTGTCGCTTTATTCTTGGGAATGAATGCTGTGAAAGATTGGCATATTATGGGATGAGTACCAATCTAGTGAACTATCTTCAAATACGTCTTAACATGGACAATGTAGCTGCTTCAAATAGTGTTACTAGTTGGTCAGGAACCTGCTACCTTACACCATTGATTGGAGCTTTCTTGGCTGATGCTTACTTGGGAAGATATTGGACTATTGCTAGTTTCTCAATCTTCTATGTCTTTGGCATGACATTGTTGACGATGGCTGCTTCAGTCCCTGGATTGAAGCCATCTTGTGACAATACTGTTTGCCATCCAACTGGAGGTCAAACGGCTGTTACCTTCATAGCACTTTACTTGATCGCCCTCGGGACCGGGGGAATCAAGCCATGTGTGTCGTCGTTTGGGGCCGATCAATTCGACGAAAATGATGAAGGCGAAAGGAAGAAGAAAAGCTCCTTCTTCAATTGGTTTTACTTTTCAATCAACGTTGGTGCAATGATTGCCTCCTCAGTTCTAGTATGGATACAAATGAATGTAGGGTGGGGGTGGGGCTTTGGAGTTCCTGCAGTAGCCATGGCCATTGCTGTAGTGTTTTTCTTCTCCGGTAGCTCGTTGTATCGTCTGCAAAAGCCAGCAGGAAGTCCTCTCACAAGAATCTTGCAAGTTATAGTTGCAGCTTGTAGGAAACAAGGAGTACAAGTTCCAGAGGATAAGTCTCTCCTTTACGAGACTGCTGATGATGTCGAGTCGAAAATCGAAGGAAGTCGCAAGCTCGAGCACACGAACGAGTTAAAGTTCCTAGATAAGGCTTGTGTATTGAGGGAAAGTGATGGAATGAAGGGGCAAGGGAATGCATGGATACTTTGTACAGTGACACAAGTTGAGGAGCTGAAGAGCATTATCAGGTTGTTGCCTGTGTGGGCGTGTGGAATAGTATTTTCAGCGGTGTATAGCCAAATGAGCACCATGTTTGTTCTACAAGGTAATATAATGGACCAGCACATTGGGGCAAGTTTCAAAATTCCATCGGCCTCGCTTTCGATCTTCGATACCCTCAGCGTTCTCTTTTGGGCTCCCGTATACGACAGACTCATCGTCCCAATGGCGAAGAAGTTCACGAACAATGAGCGTGGCTTCACACAGCTACAACGGATGGGAATAGGACTTGTCATATCTGTTTTCTCCATGGTTGCAGCAGGTGTGTTGGAAGTTTTCAGGCTGGAGTATGTGAGGAAGAACAAGCTATACGATGCGGAGTACATTCCGATGTCGATATTCTGGCAAGTTCCACAGTATTTTTTGATCGGATGTGCAGAAGTGTTCACTTTTATAGGACAGTTAGAGTTTTTCTATGACCAAGCACCGGACGCAATGAGAAGCATGATGGCTGCCCTGTCGCTCACAACGGTTGGATTGGGAAACTACTTAAGCACATTGCTCGTTACAATCGTGACGGCCGTGACAACGAGACACGGGAAGCTCGGGTGGATTCCAAATAACTTAAACAAGGGACATCTCGACTATTTCTTCTGGCTGTTGGCCATTCTCAGCGTCCTCAACTTCTTTGCCTATCTCTTTGTGGCTAAGTGCTATCGCTACAAGAAGGTAACAGGTCATTTGCGCTGA

Protein sequence

MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRLNMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVPGLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSSFFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPAGSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACVLRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMDQHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVISVFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYDQAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWLLAILSVLNFFAYLFVAKCYRYKKVTGHLR
Homology
BLAST of Carg01091 vs. NCBI nr
Match: KAG7027910.1 (Protein NRT1/ PTR FAMILY 8.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 569/569 (100.00%), Postives = 569/569 (100.00%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP
Sbjct: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS
Sbjct: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV
Sbjct: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
           LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD
Sbjct: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS
Sbjct: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD
Sbjct: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL
Sbjct: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 570
           LAILSVLNFFAYLFVAKCYRYKKVTGHLR
Sbjct: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 569

BLAST of Carg01091 vs. NCBI nr
Match: KAG6596360.1 (Protein NRT1/ PTR FAMILY 8.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1140.9 bits (2950), Expect = 0.0e+00
Identity = 568/569 (99.82%), Postives = 568/569 (99.82%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP
Sbjct: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS
Sbjct: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV
Sbjct: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
           LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD
Sbjct: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS
Sbjct: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD
Sbjct: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVT VTTRHGKLGWIPNNLNKGHLDYFFWL
Sbjct: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTTVTTRHGKLGWIPNNLNKGHLDYFFWL 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 570
           LAILSVLNFFAYLFVAKCYRYKKVTGHLR
Sbjct: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 569

BLAST of Carg01091 vs. NCBI nr
Match: XP_023539304.1 (protein NRT1/ PTR FAMILY 8.1-like [Cucurbita pepo subsp. pepo] >XP_023539305.1 protein NRT1/ PTR FAMILY 8.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 566/569 (99.47%), Postives = 566/569 (99.47%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP
Sbjct: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS
Sbjct: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSPLTRILQVIVAACRK GVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV
Sbjct: 241 GSPLTRILQVIVAACRKHGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
           LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD
Sbjct: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS
Sbjct: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD
Sbjct: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVT VTTRHGKLGWIPNNLNKGHLDYFFWL
Sbjct: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTTVTTRHGKLGWIPNNLNKGHLDYFFWL 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 570
           LAILSVLNFFAYLF AKCYRYKKVTGHLR
Sbjct: 541 LAILSVLNFFAYLFAAKCYRYKKVTGHLR 569

BLAST of Carg01091 vs. NCBI nr
Match: XP_022937913.1 (protein NRT1/ PTR FAMILY 8.1-like [Cucurbita moschata] >XP_022937922.1 protein NRT1/ PTR FAMILY 8.1-like [Cucurbita moschata])

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 565/569 (99.30%), Postives = 566/569 (99.47%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP
Sbjct: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCD+TVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDE ERKKKSS
Sbjct: 121 GLKPSCDDTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEDERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNE KFLDKACV
Sbjct: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNEFKFLDKACV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
           LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD
Sbjct: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS
Sbjct: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD
Sbjct: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVT VTTRHGKLGWIPNNLNKGHLDYFFWL
Sbjct: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTTVTTRHGKLGWIPNNLNKGHLDYFFWL 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 570
           LAILSVLNFFAYLFVAKCYRYKKVTGHLR
Sbjct: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 569

BLAST of Carg01091 vs. NCBI nr
Match: XP_022971406.1 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 8.1-like [Cucurbita maxima])

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 559/569 (98.24%), Postives = 561/569 (98.59%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAAS P
Sbjct: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASDP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS
Sbjct: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVES IEGSRKLEH NELKFLDKACV
Sbjct: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVES-IEGSRKLEHMNELKFLDKACV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
           LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMF+LQGNIMD
Sbjct: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFILQGNIMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNE GFTQLQRMGIGLVIS
Sbjct: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNEPGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQ PQYFLIGCAEVFTFIGQLEFFYD
Sbjct: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQAPQYFLIGCAEVFTFIGQLEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMMAALSL  VGLGNYLSTLLVTIVT +TTRHGKLGWIPNNLNKGHLDYFFWL
Sbjct: 481 QAPDAMRSMMAALSLILVGLGNYLSTLLVTIVTTMTTRHGKLGWIPNNLNKGHLDYFFWL 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 570
           LAILSVLNFFAYLFVAKCYRYKKVTGHLR
Sbjct: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 568

BLAST of Carg01091 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 2.9e-254
Identity = 429/568 (75.53%), Postives = 491/568 (86.44%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           M E DVYT+DGT+DIHKNPANK+KTG WKACRFILGNECCERLAYYGM TNLVNYL+ RL
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           N  N  A+N+VT+WSGTCY+TPLIGAF+ADAYLGRYWTIA+F   YV GMTLLT++ASVP
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 121 GLKP-SCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKS 180
           GLKP +C+   CHP   QTAV F+ALY+IALGTGGIKPCVSSFGADQFDENDE E+ KKS
Sbjct: 121 GLKPGNCNADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 180

Query: 181 SFFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKP 240
           SFFNWFYFSINVGA+IA++VLVWIQMNVGWGWGFGVP VAM IAV FFF GS  YRLQ+P
Sbjct: 181 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 240

Query: 241 AGSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKAC 300
            GSPLTRI QVIVAA RK  V+VPEDKSLL+ETADD ES I+GSRKL HT+ LKF DKA 
Sbjct: 241 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADD-ESNIKGSRKLVHTDNLKFFDKAA 300

Query: 301 VLRESDGMK-GQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNI 360
           V  +SD +K G+ N W LC+VTQVEELKSII LLPVWA GIVF+ VYSQMSTMFVLQGN 
Sbjct: 301 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 360

Query: 361 MDQHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLV 420
           MDQH+G +F+IPSASLS+FDT+SVLFW PVYD+ I+P+A+KFT NERGFTQLQRMGIGLV
Sbjct: 361 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 420

Query: 421 ISVFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFF 480
           +S+F+M+ AGVLEV RL+YV+ +  YD + I MSIFWQ+PQY LIGCAEVFTFIGQLEFF
Sbjct: 421 VSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 480

Query: 481 YDQAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFF 540
           YDQAPDAMRS+ +ALSLTTV LGNYLST+LVT+V  +T ++GK GWIP+NLN+GHLDYFF
Sbjct: 481 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFF 540

Query: 541 WLLAILSVLNFFAYLFVAKCYRYKKVTG 567
           +LLA LS LNF  YL+++K Y+YKK  G
Sbjct: 541 YLLATLSFLNFLVYLWISKRYKYKKAVG 567

BLAST of Carg01091 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 4.1e-253
Identity = 425/566 (75.09%), Postives = 486/566 (85.87%), Query Frame = 0

Query: 3   EDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRLNM 62
           + D+YTKDGT+DIHK PANK KTG WKACRFILG ECCERLAYYGMSTNL+NYL+ ++NM
Sbjct: 4   DKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNM 63

Query: 63  DNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVPGL 122
           +NV+AS SV++WSGTCY TPLIGAF+ADAYLGRYWTIASF + Y+ GMTLLT++ASVPGL
Sbjct: 64  ENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL 123

Query: 123 KPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSSFF 182
            P+C    CH T GQTA+TFIALYLIALGTGGIKPCVSSFGADQFD+ DE E++ KSSFF
Sbjct: 124 TPTCSGETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 183

Query: 183 NWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPAGS 242
           NWFYF INVGAMIASSVLVWIQMNVGWGWG GVP VAMAIAVVFFF+GS+ YRLQKP GS
Sbjct: 184 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 243

Query: 243 PLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACVLR 302
           PLTR+LQVIVA+CRK  V++PED+SLLYE   D ES I GSRKLEHT  L F DKA V  
Sbjct: 244 PLTRMLQVIVASCRKSKVKIPEDESLLYEN-QDAESSIIGSRKLEHTKILTFFDKAAVET 303

Query: 303 ESDGM-KGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMDQ 362
           ESD     + ++W LCTVTQVEELK++IRLLP+WA GIVF++VYSQM T+FVLQGN +DQ
Sbjct: 304 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 363

Query: 363 HIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVISV 422
           H+G +FKIPSASLS+FDTLSVLFWAPVYD+LIVP A+K+T +ERGFTQLQR+GIGLVIS+
Sbjct: 364 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 423

Query: 423 FSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYDQ 482
           FSMV+AG+LEV RL YV+ + LY+ E IPM+IFWQVPQYFL+GCAEVFTFIGQLEFFYDQ
Sbjct: 424 FSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQ 483

Query: 483 APDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWLL 542
           APDAMRS+ +ALSLT +  GNYLST LVT+VT VT   G+ GWI  NLN GHLDYFFWLL
Sbjct: 484 APDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLL 543

Query: 543 AILSVLNFFAYLFVAKCYRYKKVTGH 568
           A LS LNF  YL++AK Y YKK TGH
Sbjct: 544 AGLSFLNFLVYLWIAKWYTYKKTTGH 568

BLAST of Carg01091 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 1.9e-197
Identity = 343/569 (60.28%), Postives = 433/569 (76.10%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           + E  +Y +DG++D + NP  K+KTG WKAC FILGNECCERLAYYG++ NL+ YL  +L
Sbjct: 18  LQEVKLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKL 77

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           +  NV+A+ +VT+W GTCYLTPLIGA LADAY GRYWTIA FS  Y  GM+ LT++ASVP
Sbjct: 78  HQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVP 137

Query: 121 GLKPS-CDNTVC-HPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKK 180
            LKP+ C    C   T  Q A+ F  LYLIALGTGGIKPCVSSFGADQFD+ D  ER +K
Sbjct: 138 ALKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRK 197

Query: 181 SSFFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQK 240
           +SFFNWFYFSIN+GA+++SS+LVWIQ N GWG GFG+P V M +A+  FF G+ LYR QK
Sbjct: 198 ASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQK 257

Query: 241 PAGSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKA 300
           P GSP+TRI QV+VA+ RK  V+VPED +LLYET  D  S I GSRK+EHT++ ++LDKA
Sbjct: 258 PGGSPITRISQVVVASFRKSSVKVPEDATLLYET-QDKNSAIAGSRKIEHTDDCQYLDKA 317

Query: 301 CVLRESDGMKGQ-GNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGN 360
            V+ E +   G   N+W LCTVTQVEELK +IR+ P+WA GI+FSAVY+QMSTMFV QG 
Sbjct: 318 AVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGR 377

Query: 361 IMDQHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGL 420
            M+  IG SF++P A+L  FDT SV+ W P+YDR IVP+A+KFT  ++GFT++QRMGIGL
Sbjct: 378 AMNCKIG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGL 437

Query: 421 VISVFSMVAAGVLEVFRLEYVRKNKLYDA-EYIPMSIFWQVPQYFLIGCAEVFTFIGQLE 480
            +SV  M AA ++E+ RL       L ++   +P+S+ WQ+PQYF++G AEVF FIGQLE
Sbjct: 438 FVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLE 497

Query: 481 FFYDQAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDY 540
           FFYDQ+PDAMRS+ +AL+L T  LGNYLS+L++T+VT  TTR+G+ GWI +NLN GHLDY
Sbjct: 498 FFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDY 557

Query: 541 FFWLLAILSVLNFFAYLFVAKCYRYKKVT 566
           FFWLLA LS++N   Y F A  Y+ KK +
Sbjct: 558 FFWLLAGLSLVNMAVYFFSAARYKQKKAS 584

BLAST of Carg01091 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 1.0e-179
Identity = 317/561 (56.51%), Postives = 410/561 (73.08%), Query Frame = 0

Query: 9   KDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRLNMDNVAAS 68
           +DG+IDI+ NP +KKKTG WKAC FILGNECCERLAYYG++ NL+ Y    L+  NV+A+
Sbjct: 37  EDGSIDIYGNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAA 96

Query: 69  NSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVPGLKPSC-- 128
           + V  W GTCY+TPLIGA +AD+Y GRYWTIASFS  Y  GM LLT++AS+P LKP+   
Sbjct: 97  SDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACA 156

Query: 129 --DNTVCHP-TGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSSFFN 188
                +C P T  Q AV F  LYLIALGTGGIKPCVSSFGADQFD+ D  ER +K+SFFN
Sbjct: 157 GVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFN 216

Query: 189 WFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPAGSP 248
           WFYFSIN+G+ I+S++LVW+Q NVGWG GF +P V M +++  FF G+ LYR QKP GSP
Sbjct: 217 WFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSP 276

Query: 249 LTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACVLRE 308
           +TR+ QV+VAA RK  + +PED S LYET +   S I GSRK++HT+  KFLDKA V+ E
Sbjct: 277 ITRVCQVLVAAYRKLKLNLPEDISFLYETREK-NSMIAGSRKIQHTDGYKFLDKAAVISE 336

Query: 309 SDGMKGQ-GNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMDQH 368
            +   G   N W LCTVTQVEE+K++IR+ P+WA GIV+S +YSQ+ST+FV QG  M++ 
Sbjct: 337 YESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNRI 396

Query: 369 IGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVISVF 428
           I  SF+IP AS  +FDTL VL   P+YDR +VP  ++FT   +G T LQRMGIGL +SV 
Sbjct: 397 I-RSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVL 456

Query: 429 SMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYDQA 488
           S+ AA ++E  RL+  +       +++ MSIFWQ+PQY L+G AEVF FIG++EFFYD++
Sbjct: 457 SIAAAAIVETVRLQLAQ-------DFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDES 516

Query: 489 PDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWLLA 548
           PDAMRS+ +AL+L    +G+YLS+L++T+V   T   GK GW+P++LNKGHLDYFFWLL 
Sbjct: 517 PDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLV 576

Query: 549 ILSVLNFFAYLFVAKCYRYKK 564
            L ++N   Y  +   +  KK
Sbjct: 577 SLGLVNIPVYALICVKHTKKK 588

BLAST of Carg01091 vs. ExPASy Swiss-Prot
Match: Q84WG0 (Protein NRT1/ PTR FAMILY 8.4 OS=Arabidopsis thaliana OX=3702 GN=NPF8.4 PE=2 SV=2)

HSP 1 Score: 573.9 bits (1478), Expect = 2.0e-162
Identity = 295/566 (52.12%), Postives = 388/566 (68.55%), Query Frame = 0

Query: 3   EDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRLNM 62
           E  +Y +DG+IDIH NP  K+ TG WKAC FI  NECCERLAYYG++ NL+ Y    L+ 
Sbjct: 21  EVKLYAEDGSIDIHGNPPLKQTTGNWKACPFIFANECCERLAYYGIAKNLITYFTNELHE 80

Query: 63  DNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVPGL 122
            NV+A+  V +W GTCY+TPLIGA +ADAY GRYWTIA FS  Y  GM  LT++ASVPGL
Sbjct: 81  TNVSAARHVMTWQGTCYITPLIGALIADAYWGRYWTIACFSAIYFTGMVALTLSASVPGL 140

Query: 123 KPS-CDNTVCHP-TGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 182
           KP+ C  ++C P T  Q+ V F  LYLIALGTGGIKPCVSSFGADQFD+ D  ER +K+S
Sbjct: 141 KPAECIGSLCPPATMVQSTVLFSGLYLIALGTGGIKPCVSSFGADQFDKTDPSERVRKAS 200

Query: 183 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 242
           FFNWFYF+IN+GA ++S+VLVWIQ N GW  GF +P V M +A + FF G+ LYR QKP 
Sbjct: 201 FFNWFYFTINIGAFVSSTVLVWIQENYGWELGFLIPTVFMGLATMSFFFGTPLYRFQKPR 260

Query: 243 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 302
           GSP+T + QV+VAA RK  ++VPED                                   
Sbjct: 261 GSPITSVCQVLVAAYRKSNLKVPED----------------------------------- 320

Query: 303 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 362
              +D      N W LCTVTQVEE+K ++RL+P+WA GI+FS ++SQ+ T+FV QG  M 
Sbjct: 321 --STDEGDANTNPWKLCTVTQVEEVKILLRLVPIWASGIIFSVLHSQIYTLFVQQGRCMK 380

Query: 363 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 422
           + IG  F+IP A+L +FDT SVL   P+YDR+IVP+ ++FT   +GFT+LQRMGIGL +S
Sbjct: 381 RTIGL-FEIPPATLGMFDTASVLISVPIYDRVIVPLVRRFTGLAKGFTELQRMGIGLFVS 440

Query: 423 VFSMVAAGVLEVFRLEYVRKNKLYDA-EYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 482
           V S+  A ++E  RL+  R   L ++ + +P++IFWQ+PQYFL+G A VF F+G++EFFY
Sbjct: 441 VLSLTFAAIVETVRLQLARDLDLVESGDIVPLNIFWQIPQYFLMGTAGVFFFVGRIEFFY 500

Query: 483 DQAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIP-NNLNKGHLDYFF 542
           +Q+PD+MRS+ +A +L T  LGNYLS+L++T+V  ++   GK  WIP +N+N GHLDYFF
Sbjct: 501 EQSPDSMRSLCSAWALLTTTLGNYLSSLIITLVAYLS---GKDCWIPSDNINNGHLDYFF 545

Query: 543 WLLAILSVLNFFAYLFVAKCYRYKKV 565
           WLL  L  +N   ++F +  Y + KV
Sbjct: 561 WLLVSLGSVNIPVFVFFSVKYTHMKV 545

BLAST of Carg01091 vs. ExPASy TrEMBL
Match: A0A6J1FCK0 (protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita moschata OX=3662 GN=LOC111444159 PE=3 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 565/569 (99.30%), Postives = 566/569 (99.47%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP
Sbjct: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCD+TVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDE ERKKKSS
Sbjct: 121 GLKPSCDDTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEDERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNE KFLDKACV
Sbjct: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNEFKFLDKACV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
           LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD
Sbjct: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS
Sbjct: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD
Sbjct: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVT VTTRHGKLGWIPNNLNKGHLDYFFWL
Sbjct: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTTVTTRHGKLGWIPNNLNKGHLDYFFWL 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 570
           LAILSVLNFFAYLFVAKCYRYKKVTGHLR
Sbjct: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 569

BLAST of Carg01091 vs. ExPASy TrEMBL
Match: A0A6J1I385 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=3661 GN=LOC111470138 PE=3 SV=1)

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 559/569 (98.24%), Postives = 561/569 (98.59%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAAS P
Sbjct: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASDP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS
Sbjct: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVES IEGSRKLEH NELKFLDKACV
Sbjct: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVES-IEGSRKLEHMNELKFLDKACV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
           LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMF+LQGNIMD
Sbjct: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFILQGNIMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNE GFTQLQRMGIGLVIS
Sbjct: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNEPGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQ PQYFLIGCAEVFTFIGQLEFFYD
Sbjct: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQAPQYFLIGCAEVFTFIGQLEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMMAALSL  VGLGNYLSTLLVTIVT +TTRHGKLGWIPNNLNKGHLDYFFWL
Sbjct: 481 QAPDAMRSMMAALSLILVGLGNYLSTLLVTIVTTMTTRHGKLGWIPNNLNKGHLDYFFWL 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 570
           LAILSVLNFFAYLFVAKCYRYKKVTGHLR
Sbjct: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 568

BLAST of Carg01091 vs. ExPASy TrEMBL
Match: A0A6J1F6S4 (protein NRT1/ PTR FAMILY 8.2-like OS=Cucurbita moschata OX=3662 GN=LOC111441392 PE=3 SV=1)

HSP 1 Score: 1035.4 bits (2676), Expect = 8.8e-299
Identity = 513/569 (90.16%), Postives = 536/569 (94.20%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAEDD+YTKDGT+D+ KNPANKKKTG WKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEDDIYTKDGTVDMRKNPANKKKTGNWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NMDNVAASNSVTSWSGTCY+TPLIGAFLADAYLGRYWTIASFSI YVFGMT LTMAASVP
Sbjct: 61  NMDNVAASNSVTSWSGTCYITPLIGAFLADAYLGRYWTIASFSILYVFGMTSLTMAASVP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCD+T CHPTGGQTA TFIALYLIALGTGGIKPCVSSFGADQFDE DE ERKKKSS
Sbjct: 121 GLKPSCDSTGCHPTGGQTAATFIALYLIALGTGGIKPCVSSFGADQFDETDEVERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSPLTRILQVI+AACRK GV+VPEDKSLLYETADDVESKIEGSRKLEHTN+LKFLDKA V
Sbjct: 241 GSPLTRILQVIIAACRKHGVRVPEDKSLLYETADDVESKIEGSRKLEHTNKLKFLDKASV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
             E+D MKGQ +AW LCTVTQVEELKSI+RLLPVWA GIVFSAVY QMSTMFVLQGN MD
Sbjct: 301 ETENDRMKGQRDAWRLCTVTQVEELKSIVRLLPVWASGIVFSAVYGQMSTMFVLQGNTMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIG SFKIPSASLSIFDTLSVLFWAPVYD+LIVP+A+KFT NERGFTQLQRMGIGLVIS
Sbjct: 361 QHIGPSFKIPSASLSIFDTLSVLFWAPVYDKLIVPLARKFTKNERGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSMV AG LEV RL+YVRKN LYDAE IPMSIFWQ+PQYF IGCAEVFTFIGQ+EFFYD
Sbjct: 421 VFSMVTAGGLEVVRLKYVRKNNLYDAENIPMSIFWQIPQYFFIGCAEVFTFIGQMEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMM+ALSLTTVGLGNYLSTL+VTIVT V+TRHGKLGWIP+NLNKGHLDYFFWL
Sbjct: 481 QAPDAMRSMMSALSLTTVGLGNYLSTLIVTIVTKVSTRHGKLGWIPSNLNKGHLDYFFWL 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 570
           LAILSV+NFF YL VAK Y  K+ TGHLR
Sbjct: 541 LAILSVVNFFVYLLVAKSYTSKRATGHLR 569

BLAST of Carg01091 vs. ExPASy TrEMBL
Match: A0A6J1CX33 (protein NRT1/ PTR FAMILY 8.2-like OS=Momordica charantia OX=3673 GN=LOC111015090 PE=3 SV=1)

HSP 1 Score: 1033.1 bits (2670), Expect = 4.4e-298
Identity = 509/569 (89.46%), Postives = 537/569 (94.38%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAE+D+YTKDGT+DIHKNPANK KTG WKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEEDIYTKDGTVDIHKNPANKNKTGNWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NM NVAASNSVT+WSGTCYLTPLIGAFLADAY+GRYWTIASFSI YVFGM LLTMAASVP
Sbjct: 61  NMGNVAASNSVTTWSGTCYLTPLIGAFLADAYMGRYWTIASFSILYVFGMALLTMAASVP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCD + CHPTGGQTA TFIALYLIALGTGGIKPCVSSFGADQFD+NDE ERKKKSS
Sbjct: 121 GLKPSCDGSDCHPTGGQTAATFIALYLIALGTGGIKPCVSSFGADQFDDNDEAERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGA+IASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGALIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSP+TRILQVIVA+ RKQ + +PEDKSLLYET+DDVESKIEGSRKLEHTN+LKFLDKA V
Sbjct: 241 GSPITRILQVIVASIRKQQIHIPEDKSLLYETSDDVESKIEGSRKLEHTNKLKFLDKAAV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
             ++D +KGQ +AW LCTVTQVEELKS+I LLPVWACGIVFSAVYSQMSTMFVLQGN MD
Sbjct: 301 ESQTDWIKGQPSAWRLCTVTQVEELKSVIGLLPVWACGIVFSAVYSQMSTMFVLQGNTMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIG  FKIPSASLSIFDTLSVLFWAPVYDR+IVP+A+KFT NERGFTQLQRMGIGLVIS
Sbjct: 361 QHIGPHFKIPSASLSIFDTLSVLFWAPVYDRVIVPVARKFTGNERGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSM+AAG+LEV RL+YVRKN LY+ E IPMSIFWQVP YFLIGCAEVFTFIGQLEFFYD
Sbjct: 421 VFSMIAAGILEVVRLDYVRKNNLYEVETIPMSIFWQVPPYFLIGCAEVFTFIGQLEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVT VTTRHGKLGWIP+NLNKGHLDYFFW+
Sbjct: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTEVTTRHGKLGWIPDNLNKGHLDYFFWV 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHLR 570
           LAILSVLNFFAYL VAK Y YKKVTGHLR
Sbjct: 541 LAILSVLNFFAYLLVAKFYTYKKVTGHLR 569

BLAST of Carg01091 vs. ExPASy TrEMBL
Match: A0A6J1J7E4 (protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=3661 GN=LOC111481926 PE=3 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.3e-297
Identity = 511/568 (89.96%), Postives = 535/568 (94.19%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           MAEDD+YTKDGT+D+ KNPANKKKTG WKACRFILGNECCERLAYYGMSTNLVNYLQIRL
Sbjct: 1   MAEDDIYTKDGTVDMRKNPANKKKTGNWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           NMDNVAASNSVTSWSGTCY+TPLIGAFLADAYLGRYWTIASFSI YVFGMTLLTMAASVP
Sbjct: 61  NMDNVAASNSVTSWSGTCYITPLIGAFLADAYLGRYWTIASFSILYVFGMTLLTMAASVP 120

Query: 121 GLKPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 180
           GLKPSCD+T CHP GGQTA TF+ALYLIALGTGGIKPCVSSFGADQFDE DE ERKKKSS
Sbjct: 121 GLKPSCDSTGCHPMGGQTAATFMALYLIALGTGGIKPCVSSFGADQFDETDEVERKKKSS 180

Query: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 240
           FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMA+AVVFFFSGSSLYRLQKPA
Sbjct: 181 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAVAVVFFFSGSSLYRLQKPA 240

Query: 241 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 300
           GSPLTRILQVIVAACRK GV+VPEDKSLLYETADDVESKIEGSRKLEHTN+LKFLDKA V
Sbjct: 241 GSPLTRILQVIVAACRKHGVRVPEDKSLLYETADDVESKIEGSRKLEHTNKLKFLDKASV 300

Query: 301 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 360
             E+D MKGQ +AW LCTVTQVEELKSI+RLLPVWA GIVFSAVYSQMSTMFVLQGN MD
Sbjct: 301 ETENDRMKGQIDAWSLCTVTQVEELKSIVRLLPVWASGIVFSAVYSQMSTMFVLQGNTMD 360

Query: 361 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 420
           QHIG SFKIPSASLSIFDTLSVLFWAPVYD+LIVP+A+KFT NERGFTQLQRMGIGLVIS
Sbjct: 361 QHIGPSFKIPSASLSIFDTLSVLFWAPVYDKLIVPLARKFTKNERGFTQLQRMGIGLVIS 420

Query: 421 VFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYD 480
           VFSMV AG LEV RL+YVRKN LY+ E IPMSIFWQ+PQYF IGCAEVFTFIGQ+EFFYD
Sbjct: 421 VFSMVTAGGLEVVRLKYVRKNNLYEVENIPMSIFWQIPQYFFIGCAEVFTFIGQMEFFYD 480

Query: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWL 540
           QAPDAMRSMMAALSLTTVGLGNYLSTL+VTIVT V+TRHGKLGWIP+NLNKGHLDYFFWL
Sbjct: 481 QAPDAMRSMMAALSLTTVGLGNYLSTLIVTIVTKVSTRHGKLGWIPSNLNKGHLDYFFWL 540

Query: 541 LAILSVLNFFAYLFVAKCYRYKKVTGHL 569
           LAILSV+NFF YL VAK Y  K+ TGHL
Sbjct: 541 LAILSVVNFFVYLLVAKSYTSKRATGHL 568

BLAST of Carg01091 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 879.0 bits (2270), Expect = 2.0e-255
Identity = 429/568 (75.53%), Postives = 491/568 (86.44%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           M E DVYT+DGT+DIHKNPANK+KTG WKACRFILGNECCERLAYYGM TNLVNYL+ RL
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           N  N  A+N+VT+WSGTCY+TPLIGAF+ADAYLGRYWTIA+F   YV GMTLLT++ASVP
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 121 GLKP-SCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKS 180
           GLKP +C+   CHP   QTAV F+ALY+IALGTGGIKPCVSSFGADQFDENDE E+ KKS
Sbjct: 121 GLKPGNCNADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 180

Query: 181 SFFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKP 240
           SFFNWFYFSINVGA+IA++VLVWIQMNVGWGWGFGVP VAM IAV FFF GS  YRLQ+P
Sbjct: 181 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 240

Query: 241 AGSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKAC 300
            GSPLTRI QVIVAA RK  V+VPEDKSLL+ETADD ES I+GSRKL HT+ LKF DKA 
Sbjct: 241 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADD-ESNIKGSRKLVHTDNLKFFDKAA 300

Query: 301 VLRESDGMK-GQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNI 360
           V  +SD +K G+ N W LC+VTQVEELKSII LLPVWA GIVF+ VYSQMSTMFVLQGN 
Sbjct: 301 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 360

Query: 361 MDQHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLV 420
           MDQH+G +F+IPSASLS+FDT+SVLFW PVYD+ I+P+A+KFT NERGFTQLQRMGIGLV
Sbjct: 361 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 420

Query: 421 ISVFSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFF 480
           +S+F+M+ AGVLEV RL+YV+ +  YD + I MSIFWQ+PQY LIGCAEVFTFIGQLEFF
Sbjct: 421 VSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 480

Query: 481 YDQAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFF 540
           YDQAPDAMRS+ +ALSLTTV LGNYLST+LVT+V  +T ++GK GWIP+NLN+GHLDYFF
Sbjct: 481 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFF 540

Query: 541 WLLAILSVLNFFAYLFVAKCYRYKKVTG 567
           +LLA LS LNF  YL+++K Y+YKK  G
Sbjct: 541 YLLATLSFLNFLVYLWISKRYKYKKAVG 567

BLAST of Carg01091 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 875.2 bits (2260), Expect = 2.9e-254
Identity = 425/566 (75.09%), Postives = 486/566 (85.87%), Query Frame = 0

Query: 3   EDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRLNM 62
           + D+YTKDGT+DIHK PANK KTG WKACRFILG ECCERLAYYGMSTNL+NYL+ ++NM
Sbjct: 4   DKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNM 63

Query: 63  DNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVPGL 122
           +NV+AS SV++WSGTCY TPLIGAF+ADAYLGRYWTIASF + Y+ GMTLLT++ASVPGL
Sbjct: 64  ENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL 123

Query: 123 KPSCDNTVCHPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSSFF 182
            P+C    CH T GQTA+TFIALYLIALGTGGIKPCVSSFGADQFD+ DE E++ KSSFF
Sbjct: 124 TPTCSGETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 183

Query: 183 NWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPAGS 242
           NWFYF INVGAMIASSVLVWIQMNVGWGWG GVP VAMAIAVVFFF+GS+ YRLQKP GS
Sbjct: 184 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 243

Query: 243 PLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACVLR 302
           PLTR+LQVIVA+CRK  V++PED+SLLYE   D ES I GSRKLEHT  L F DKA V  
Sbjct: 244 PLTRMLQVIVASCRKSKVKIPEDESLLYEN-QDAESSIIGSRKLEHTKILTFFDKAAVET 303

Query: 303 ESDGM-KGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMDQ 362
           ESD     + ++W LCTVTQVEELK++IRLLP+WA GIVF++VYSQM T+FVLQGN +DQ
Sbjct: 304 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 363

Query: 363 HIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVISV 422
           H+G +FKIPSASLS+FDTLSVLFWAPVYD+LIVP A+K+T +ERGFTQLQR+GIGLVIS+
Sbjct: 364 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 423

Query: 423 FSMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYDQ 482
           FSMV+AG+LEV RL YV+ + LY+ E IPM+IFWQVPQYFL+GCAEVFTFIGQLEFFYDQ
Sbjct: 424 FSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQ 483

Query: 483 APDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWLL 542
           APDAMRS+ +ALSLT +  GNYLST LVT+VT VT   G+ GWI  NLN GHLDYFFWLL
Sbjct: 484 APDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLL 543

Query: 543 AILSVLNFFAYLFVAKCYRYKKVTGH 568
           A LS LNF  YL++AK Y YKK TGH
Sbjct: 544 AGLSFLNFLVYLWIAKWYTYKKTTGH 568

BLAST of Carg01091 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 690.3 bits (1780), Expect = 1.3e-198
Identity = 343/569 (60.28%), Postives = 433/569 (76.10%), Query Frame = 0

Query: 1   MAEDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRL 60
           + E  +Y +DG++D + NP  K+KTG WKAC FILGNECCERLAYYG++ NL+ YL  +L
Sbjct: 18  LQEVKLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKL 77

Query: 61  NMDNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVP 120
           +  NV+A+ +VT+W GTCYLTPLIGA LADAY GRYWTIA FS  Y  GM+ LT++ASVP
Sbjct: 78  HQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVP 137

Query: 121 GLKPS-CDNTVC-HPTGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKK 180
            LKP+ C    C   T  Q A+ F  LYLIALGTGGIKPCVSSFGADQFD+ D  ER +K
Sbjct: 138 ALKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRK 197

Query: 181 SSFFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQK 240
           +SFFNWFYFSIN+GA+++SS+LVWIQ N GWG GFG+P V M +A+  FF G+ LYR QK
Sbjct: 198 ASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQK 257

Query: 241 PAGSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKA 300
           P GSP+TRI QV+VA+ RK  V+VPED +LLYET  D  S I GSRK+EHT++ ++LDKA
Sbjct: 258 PGGSPITRISQVVVASFRKSSVKVPEDATLLYET-QDKNSAIAGSRKIEHTDDCQYLDKA 317

Query: 301 CVLRESDGMKGQ-GNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGN 360
            V+ E +   G   N+W LCTVTQVEELK +IR+ P+WA GI+FSAVY+QMSTMFV QG 
Sbjct: 318 AVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGR 377

Query: 361 IMDQHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGL 420
            M+  IG SF++P A+L  FDT SV+ W P+YDR IVP+A+KFT  ++GFT++QRMGIGL
Sbjct: 378 AMNCKIG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGL 437

Query: 421 VISVFSMVAAGVLEVFRLEYVRKNKLYDA-EYIPMSIFWQVPQYFLIGCAEVFTFIGQLE 480
            +SV  M AA ++E+ RL       L ++   +P+S+ WQ+PQYF++G AEVF FIGQLE
Sbjct: 438 FVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLE 497

Query: 481 FFYDQAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDY 540
           FFYDQ+PDAMRS+ +AL+L T  LGNYLS+L++T+VT  TTR+G+ GWI +NLN GHLDY
Sbjct: 498 FFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDY 557

Query: 541 FFWLLAILSVLNFFAYLFVAKCYRYKKVT 566
           FFWLLA LS++N   Y F A  Y+ KK +
Sbjct: 558 FFWLLAGLSLVNMAVYFFSAARYKQKKAS 584

BLAST of Carg01091 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 631.3 bits (1627), Expect = 7.4e-181
Identity = 317/561 (56.51%), Postives = 410/561 (73.08%), Query Frame = 0

Query: 9   KDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRLNMDNVAAS 68
           +DG+IDI+ NP +KKKTG WKAC FILGNECCERLAYYG++ NL+ Y    L+  NV+A+
Sbjct: 37  EDGSIDIYGNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAA 96

Query: 69  NSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVPGLKPSC-- 128
           + V  W GTCY+TPLIGA +AD+Y GRYWTIASFS  Y  GM LLT++AS+P LKP+   
Sbjct: 97  SDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACA 156

Query: 129 --DNTVCHP-TGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSSFFN 188
                +C P T  Q AV F  LYLIALGTGGIKPCVSSFGADQFD+ D  ER +K+SFFN
Sbjct: 157 GVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFN 216

Query: 189 WFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPAGSP 248
           WFYFSIN+G+ I+S++LVW+Q NVGWG GF +P V M +++  FF G+ LYR QKP GSP
Sbjct: 217 WFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSP 276

Query: 249 LTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACVLRE 308
           +TR+ QV+VAA RK  + +PED S LYET +   S I GSRK++HT+  KFLDKA V+ E
Sbjct: 277 ITRVCQVLVAAYRKLKLNLPEDISFLYETREK-NSMIAGSRKIQHTDGYKFLDKAAVISE 336

Query: 309 SDGMKGQ-GNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMDQH 368
            +   G   N W LCTVTQVEE+K++IR+ P+WA GIV+S +YSQ+ST+FV QG  M++ 
Sbjct: 337 YESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNRI 396

Query: 369 IGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVISVF 428
           I  SF+IP AS  +FDTL VL   P+YDR +VP  ++FT   +G T LQRMGIGL +SV 
Sbjct: 397 I-RSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVL 456

Query: 429 SMVAAGVLEVFRLEYVRKNKLYDAEYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFYDQA 488
           S+ AA ++E  RL+  +       +++ MSIFWQ+PQY L+G AEVF FIG++EFFYD++
Sbjct: 457 SIAAAAIVETVRLQLAQ-------DFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDES 516

Query: 489 PDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIPNNLNKGHLDYFFWLLA 548
           PDAMRS+ +AL+L    +G+YLS+L++T+V   T   GK GW+P++LNKGHLDYFFWLL 
Sbjct: 517 PDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLV 576

Query: 549 ILSVLNFFAYLFVAKCYRYKK 564
            L ++N   Y  +   +  KK
Sbjct: 577 SLGLVNIPVYALICVKHTKKK 588

BLAST of Carg01091 vs. TAIR 10
Match: AT2G02020.1 (Major facilitator superfamily protein )

HSP 1 Score: 573.9 bits (1478), Expect = 1.4e-163
Identity = 295/566 (52.12%), Postives = 388/566 (68.55%), Query Frame = 0

Query: 3   EDDVYTKDGTIDIHKNPANKKKTGKWKACRFILGNECCERLAYYGMSTNLVNYLQIRLNM 62
           E  +Y +DG+IDIH NP  K+ TG WKAC FI  NECCERLAYYG++ NL+ Y    L+ 
Sbjct: 21  EVKLYAEDGSIDIHGNPPLKQTTGNWKACPFIFANECCERLAYYGIAKNLITYFTNELHE 80

Query: 63  DNVAASNSVTSWSGTCYLTPLIGAFLADAYLGRYWTIASFSIFYVFGMTLLTMAASVPGL 122
            NV+A+  V +W GTCY+TPLIGA +ADAY GRYWTIA FS  Y  GM  LT++ASVPGL
Sbjct: 81  TNVSAARHVMTWQGTCYITPLIGALIADAYWGRYWTIACFSAIYFTGMVALTLSASVPGL 140

Query: 123 KPS-CDNTVCHP-TGGQTAVTFIALYLIALGTGGIKPCVSSFGADQFDENDEGERKKKSS 182
           KP+ C  ++C P T  Q+ V F  LYLIALGTGGIKPCVSSFGADQFD+ D  ER +K+S
Sbjct: 141 KPAECIGSLCPPATMVQSTVLFSGLYLIALGTGGIKPCVSSFGADQFDKTDPSERVRKAS 200

Query: 183 FFNWFYFSINVGAMIASSVLVWIQMNVGWGWGFGVPAVAMAIAVVFFFSGSSLYRLQKPA 242
           FFNWFYF+IN+GA ++S+VLVWIQ N GW  GF +P V M +A + FF G+ LYR QKP 
Sbjct: 201 FFNWFYFTINIGAFVSSTVLVWIQENYGWELGFLIPTVFMGLATMSFFFGTPLYRFQKPR 260

Query: 243 GSPLTRILQVIVAACRKQGVQVPEDKSLLYETADDVESKIEGSRKLEHTNELKFLDKACV 302
           GSP+T + QV+VAA RK  ++VPED                                   
Sbjct: 261 GSPITSVCQVLVAAYRKSNLKVPED----------------------------------- 320

Query: 303 LRESDGMKGQGNAWILCTVTQVEELKSIIRLLPVWACGIVFSAVYSQMSTMFVLQGNIMD 362
              +D      N W LCTVTQVEE+K ++RL+P+WA GI+FS ++SQ+ T+FV QG  M 
Sbjct: 321 --STDEGDANTNPWKLCTVTQVEEVKILLRLVPIWASGIIFSVLHSQIYTLFVQQGRCMK 380

Query: 363 QHIGASFKIPSASLSIFDTLSVLFWAPVYDRLIVPMAKKFTNNERGFTQLQRMGIGLVIS 422
           + IG  F+IP A+L +FDT SVL   P+YDR+IVP+ ++FT   +GFT+LQRMGIGL +S
Sbjct: 381 RTIGL-FEIPPATLGMFDTASVLISVPIYDRVIVPLVRRFTGLAKGFTELQRMGIGLFVS 440

Query: 423 VFSMVAAGVLEVFRLEYVRKNKLYDA-EYIPMSIFWQVPQYFLIGCAEVFTFIGQLEFFY 482
           V S+  A ++E  RL+  R   L ++ + +P++IFWQ+PQYFL+G A VF F+G++EFFY
Sbjct: 441 VLSLTFAAIVETVRLQLARDLDLVESGDIVPLNIFWQIPQYFLMGTAGVFFFVGRIEFFY 500

Query: 483 DQAPDAMRSMMAALSLTTVGLGNYLSTLLVTIVTAVTTRHGKLGWIP-NNLNKGHLDYFF 542
           +Q+PD+MRS+ +A +L T  LGNYLS+L++T+V  ++   GK  WIP +N+N GHLDYFF
Sbjct: 501 EQSPDSMRSLCSAWALLTTTLGNYLSSLIITLVAYLS---GKDCWIPSDNINNGHLDYFF 545

Query: 543 WLLAILSVLNFFAYLFVAKCYRYKKV 565
           WLL  L  +N   ++F +  Y + KV
Sbjct: 561 WLLVSLGSVNIPVFVFFSVKYTHMKV 545

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027910.10.0e+00100.00Protein NRT1/ PTR FAMILY 8.1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6596360.10.0e+0099.82Protein NRT1/ PTR FAMILY 8.1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023539304.10.0e+0099.47protein NRT1/ PTR FAMILY 8.1-like [Cucurbita pepo subsp. pepo] >XP_023539305.1 p... [more]
XP_022937913.10.0e+0099.30protein NRT1/ PTR FAMILY 8.1-like [Cucurbita moschata] >XP_022937922.1 protein N... [more]
XP_022971406.10.0e+0098.24LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 8.1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9M3902.9e-25475.53Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB84.1e-25375.09Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
P460321.9e-19760.28Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z201.0e-17956.51Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Q84WG02.0e-16252.12Protein NRT1/ PTR FAMILY 8.4 OS=Arabidopsis thaliana OX=3702 GN=NPF8.4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1FCK00.0e+0099.30protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita moschata OX=3662 GN=LOC111444159 ... [more]
A0A6J1I3850.0e+0098.24LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=36... [more]
A0A6J1F6S48.8e-29990.16protein NRT1/ PTR FAMILY 8.2-like OS=Cucurbita moschata OX=3662 GN=LOC111441392 ... [more]
A0A6J1CX334.4e-29889.46protein NRT1/ PTR FAMILY 8.2-like OS=Momordica charantia OX=3673 GN=LOC111015090... [more]
A0A6J1J7E41.3e-29789.96protein NRT1/ PTR FAMILY 8.1-like OS=Cucurbita maxima OX=3661 GN=LOC111481926 PE... [more]
Match NameE-valueIdentityDescription
AT3G54140.12.0e-25575.53peptide transporter 1 [more]
AT5G01180.12.9e-25475.09peptide transporter 5 [more]
AT2G02040.11.3e-19860.28peptide transporter 2 [more]
AT1G62200.17.4e-18156.51Major facilitator superfamily protein [more]
AT2G02020.11.4e-16352.12Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 96..525
e-value: 1.7E-127
score: 425.5
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 12..565
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 2..566
e-value: 1.9E-219
score: 731.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 17..559
NoneNo IPR availablePANTHERPTHR11654:SF532PROTEIN NRT1/ PTR FAMILY 8.1coord: 12..565
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 85..109
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01023PTR2_2coord: 182..194

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01091-RACarg01091-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0006857 oligopeptide transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity