Carg01070 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01070
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 5.6-like
LocationCarg_Chr06: 1361286 .. 1362576 (+)
RNA-Seq ExpressionCarg01070
SyntenyCarg01070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACAGAGGCGGAAAACAGAGGAGACGATCAAATTTTGGTTCATGATTCTTCTGTTGATCATAAACGACGGCTTCCACTCAGAGCTTCCACCGGCGTCTGGAAAGCTTCCCTCTTCATCATCGGTACCCAGTTTATAATCTCCATTGTTTTTACTTCAAAGCTTTGCCTTTTCATTCATTATCTTTTTGCTTCTCTTGAGACGAAGAGACAGAGAGAGAGAGAGAGAGAGACAGAGAGACAGAGAGACAGAGAGAGAGAGAGAGAGAGACAGAGAGAGAGGAGAATCATGTTTCTTTTCTTTAATGTGGCTGTCATCACATAAAGTCCCGACCACACGAGTTCAATCACATGAAAAGTATAAGCTAAAGCTACTATGCCTCTTGTTCTTAACTTTATGAATAATGTTCCATTATTCTTCGTCTTTTTGGTTGCAGCCATTGAGTTCAGTGAGAGGCTGAGTTACTTTGGAATAGCTACGAGTTTGGTTATATATCTCACCAGGGTCATCCGACAAGACCTCAAAACAGCGGCTAGGAATGTTAATTATTGGACTGGCGTTACCACTTTGATGCCTCTGTTTGGCGGCTTCTTGGCCGATGCTTATTTGGGTCGTTTCTCCACTGTTCTCGTTTCAACCATCGTCTACCTCTTCGTAATAATCCTTTTTCACTTCTGGGTTTCATTCAATTTATCATTTTGGTTAAGATTTTTTTATCATGTGGGTGTGTAAAATGCAGGGGTTGTCTCTGCTGACATTGTCCACTCTTGTACCAAGCTTGAAGGCTTGCGGTAGTGAGCCCTGCGATGAGCCAAGGAAGCTCCATGAAGTCTTGTTCTTCACGGCGATTTACTTAATCTCCGTTGGAACTGGTGGGCACAAACCATCTCTTGAGAGCTTTGGCGCGGACCAGTTCGATGACGATCATGCAGAGGAAAGAAAGCAAAAGATGTCGTTTTTCAATTGGTGGAACTCTGGGCTCTGTGCTGGTGTCATATTTGGAGTGACGTTAATTGTGTATGTTCAAGAACATGTCGGTTGGGGCATGGCTGGTGTGATTCTCACGTCGGTTATGGCAACATCGCTTGCTATCTTCCTTGTTGGAAGGCCGGTTTATCGGTACAGGGCGCCATTAGGAAGCCCTTTAACTCCTCTGTTGCAGGTTTTAGTGGCTGCTTTTACAAGGAGAAACTTGCCTTATCCTTCAAATTCTGCTCATCTCTATGAAGTTCAAGCTACAGACAAGGTTCATGGGAGGCTGTTGACTCACACAAACAGGCTCAAGTAA

mRNA sequence

ATGAAAACAGAGGCGGAAAACAGAGGAGACGATCAAATTTTGGTTCATGATTCTTCTGTTGATCATAAACGACGGCTTCCACTCAGAGCTTCCACCGGCGTCTGGAAAGCTTCCCTCTTCATCATCGCCATTGAGTTCAGTGAGAGGCTGAGTTACTTTGGAATAGCTACGAGTTTGGTTATATATCTCACCAGGGTCATCCGACAAGACCTCAAAACAGCGGCTAGGAATGTTAATTATTGGACTGGCGTTACCACTTTGATGCCTCTGTTTGGCGGCTTCTTGGCCGATGCTTATTTGGGTCGTTTCTCCACTGTTCTCGTTTCAACCATCGTCTACCTCTTCGGGTTGTCTCTGCTGACATTGTCCACTCTTGTACCAAGCTTGAAGGCTTGCGGTAGTGAGCCCTGCGATGAGCCAAGGAAGCTCCATGAAGTCTTGTTCTTCACGGCGATTTACTTAATCTCCGTTGGAACTGGTGGGCACAAACCATCTCTTGAGAGCTTTGGCGCGGACCAGTTCGATGACGATCATGCAGAGGAAAGAAAGCAAAAGATGTCGTTTTTCAATTGGTGGAACTCTGGGCTCTGTGCTGGTGTCATATTTGGAGTGACGTTAATTGTGTATGTTCAAGAACATGTCGGTTGGGGCATGGCTGGTGTGATTCTCACGTCGGTTATGGCAACATCGCTTGCTATCTTCCTTGTTGGAAGGCCGGTTTATCGGTACAGGGCGCCATTAGGAAGCCCTTTAACTCCTCTGTTGCAGGTTTTAGTGGCTGCTTTTACAAGGAGAAACTTGCCTTATCCTTCAAATTCTGCTCATCTCTATGAAGTTCAAGCTACAGACAAGGTTCATGGGAGGCTGTTGACTCACACAAACAGGCTCAAGTAA

Coding sequence (CDS)

ATGAAAACAGAGGCGGAAAACAGAGGAGACGATCAAATTTTGGTTCATGATTCTTCTGTTGATCATAAACGACGGCTTCCACTCAGAGCTTCCACCGGCGTCTGGAAAGCTTCCCTCTTCATCATCGCCATTGAGTTCAGTGAGAGGCTGAGTTACTTTGGAATAGCTACGAGTTTGGTTATATATCTCACCAGGGTCATCCGACAAGACCTCAAAACAGCGGCTAGGAATGTTAATTATTGGACTGGCGTTACCACTTTGATGCCTCTGTTTGGCGGCTTCTTGGCCGATGCTTATTTGGGTCGTTTCTCCACTGTTCTCGTTTCAACCATCGTCTACCTCTTCGGGTTGTCTCTGCTGACATTGTCCACTCTTGTACCAAGCTTGAAGGCTTGCGGTAGTGAGCCCTGCGATGAGCCAAGGAAGCTCCATGAAGTCTTGTTCTTCACGGCGATTTACTTAATCTCCGTTGGAACTGGTGGGCACAAACCATCTCTTGAGAGCTTTGGCGCGGACCAGTTCGATGACGATCATGCAGAGGAAAGAAAGCAAAAGATGTCGTTTTTCAATTGGTGGAACTCTGGGCTCTGTGCTGGTGTCATATTTGGAGTGACGTTAATTGTGTATGTTCAAGAACATGTCGGTTGGGGCATGGCTGGTGTGATTCTCACGTCGGTTATGGCAACATCGCTTGCTATCTTCCTTGTTGGAAGGCCGGTTTATCGGTACAGGGCGCCATTAGGAAGCCCTTTAACTCCTCTGTTGCAGGTTTTAGTGGCTGCTTTTACAAGGAGAAACTTGCCTTATCCTTCAAATTCTGCTCATCTCTATGAAGTTCAAGCTACAGACAAGGTTCATGGGAGGCTGTTGACTCACACAAACAGGCTCAAGTAA

Protein sequence

MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK
Homology
BLAST of Carg01070 vs. NCBI nr
Match: KAG6596385.1 (Protein NRT1/ PTR FAMILY 5.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 592.4 bits (1526), Expect = 2.1e-165
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60
           MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60

Query: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120
           IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL
Sbjct: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120

Query: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180
           TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE
Sbjct: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180

Query: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240

Query: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK
Sbjct: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 297

BLAST of Carg01070 vs. NCBI nr
Match: KAG7027931.1 (Protein NRT1/ PTR FAMILY 5.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.4 bits (1526), Expect = 2.1e-165
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60
           MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60

Query: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120
           IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL
Sbjct: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120

Query: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180
           TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE
Sbjct: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180

Query: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240

Query: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK
Sbjct: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 297

BLAST of Carg01070 vs. NCBI nr
Match: XP_022941773.1 (protein NRT1/ PTR FAMILY 5.6-like [Cucurbita moschata])

HSP 1 Score: 592.4 bits (1526), Expect = 2.1e-165
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60
           MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60

Query: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120
           IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL
Sbjct: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120

Query: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180
           TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE
Sbjct: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180

Query: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240

Query: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK
Sbjct: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 297

BLAST of Carg01070 vs. NCBI nr
Match: XP_023540060.1 (protein NRT1/ PTR FAMILY 5.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 586.6 bits (1511), Expect = 1.2e-163
Identity = 294/297 (98.99%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60
           MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60

Query: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120
           IYLTRVIRQDLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTVLVSTIVYLFGLSLL
Sbjct: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120

Query: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180
           TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDH E
Sbjct: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHVE 180

Query: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240

Query: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPS+SAHLYEVQATDKVHGRLLTHTNRLK
Sbjct: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSDSAHLYEVQATDKVHGRLLTHTNRLK 297

BLAST of Carg01070 vs. NCBI nr
Match: XP_022971232.1 (protein NRT1/ PTR FAMILY 5.6-like [Cucurbita maxima])

HSP 1 Score: 584.7 bits (1506), Expect = 4.4e-163
Identity = 292/297 (98.32%), Postives = 294/297 (98.99%), Query Frame = 0

Query: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60
           MKTEAEN GDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKTEAENSGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60

Query: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120
           IYLTRVIRQDLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTVL ST+VYLFGLSLL
Sbjct: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLASTVVYLFGLSLL 120

Query: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180
           TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE
Sbjct: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180

Query: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240

Query: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRL+
Sbjct: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLR 297

BLAST of Carg01070 vs. ExPASy Swiss-Prot
Match: P0CI03 (Protein NRT1/ PTR FAMILY 5.6 OS=Arabidopsis thaliana OX=3702 GN=NPF5.6 PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.3e-114
Identity = 197/288 (68.40%), Postives = 232/288 (80.56%), Query Frame = 0

Query: 10  DDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRVIRQ 69
           D Q  V DSS+D + R+PLRA TG W+A+LFIIAIEFSERLSYFG+AT+LV+YLT ++ Q
Sbjct: 17  DQQKWVLDSSLDSRGRVPLRARTGAWRAALFIIAIEFSERLSYFGLATNLVVYLTTILNQ 76

Query: 70  DLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTLVPSL 129
           DLK A RNVNYW+GVTTLMPL GGF+ADAYLGR++TVLV+T +YL GL LLT+S  +P L
Sbjct: 77  DLKMAIRNVNYWSGVTTLMPLLGGFIADAYLGRYATVLVATTIYLMGLVLLTMSWFIPGL 136

Query: 130 KACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQKMSFF 189
           K C  E C EPRK HEV FF AIYLIS+GTGGHKPSLESFGADQFDDDH EERK KMSFF
Sbjct: 137 KPCHQEVCVEPRKAHEVAFFIAIYLISIGTGGHKPSLESFGADQFDDDHVEERKMKMSFF 196

Query: 190 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRAPLGS 249
           NWWN  LCAG++  VT + Y+++ VGWG+AG+ILT VMA SL IF +G+P YRYR P GS
Sbjct: 197 NWWNVSLCAGILTAVTAVAYIEDRVGWGVAGIILTVVMAISLIIFFIGKPFYRYRTPSGS 256

Query: 250 PLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           PLTP+LQV VAA  +RNLPYPS+ + L+EV  T+   GRLL HT  LK
Sbjct: 257 PLTPILQVFVAAIAKRNLPYPSDPSLLHEVSKTEFTSGRLLCHTEHLK 304

BLAST of Carg01070 vs. ExPASy Swiss-Prot
Match: Q9M331 (Protein NRT1/ PTR FAMILY 5.7 OS=Arabidopsis thaliana OX=3702 GN=NPF5.7 PE=2 SV=2)

HSP 1 Score: 396.4 bits (1017), Expect = 2.9e-109
Identity = 190/296 (64.19%), Postives = 235/296 (79.39%), Query Frame = 0

Query: 3   TEAENRGDD-QILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVI 62
           TE ++  DD Q  V DSS D +  +PLRA TG W+A+LFII IEFSERLSYFGI+T+LV+
Sbjct: 8   TEPQDSYDDQQKWVLDSSTDSRGEIPLRAQTGAWRAALFIIGIEFSERLSYFGISTNLVV 67

Query: 63  YLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLT 122
           YLT ++ QDLK A +N NYW+GVTTLMPL GGF+ADAYLGR+ TVL++T +YL GL LLT
Sbjct: 68  YLTTILHQDLKMAVKNTNYWSGVTTLMPLLGGFVADAYLGRYGTVLLATTIYLMGLILLT 127

Query: 123 LSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEE 182
           LS  +P LKAC  + C EPRK HE+ FF AIYLIS+GTGGHKPSLESFGADQF+D H EE
Sbjct: 128 LSWFIPGLKACHEDMCVEPRKAHEIAFFIAIYLISIGTGGHKPSLESFGADQFEDGHPEE 187

Query: 183 RKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVY 242
           RK KMS+FNWWN+GLCAG++  VT+IVY+++ +GWG+A +ILT VMATS  IF +G+P Y
Sbjct: 188 RKMKMSYFNWWNAGLCAGILTAVTVIVYIEDRIGWGVASIILTIVMATSFFIFRIGKPFY 247

Query: 243 RYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           RYRAP GSPLTP+LQV VAA  +RNLP PS+S+ L+E+   +   GRLL+ +  LK
Sbjct: 248 RYRAPSGSPLTPMLQVFVAAIAKRNLPCPSDSSLLHELTNEEYTKGRLLSSSKNLK 303

BLAST of Carg01070 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.6e-57
Identity = 120/287 (41.81%), Postives = 170/287 (59.23%), Query Frame = 0

Query: 12  QILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRVIRQDL 71
           ++   D SVD     PL+  TG WKA  FI+  E  ERL+Y+GIA +L+ YLT  + Q  
Sbjct: 22  KLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGN 81

Query: 72  KTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTLVPSLK- 131
            +AA NV  W G   L PL G  LADAY GR+ T+   + +Y  G+S LTLS  VP+LK 
Sbjct: 82  VSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKP 141

Query: 132 -ACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQKMSFF 191
             C  + C         +FF  +YLI++GTGG KP + SFGADQFDD  + ER +K SFF
Sbjct: 142 AECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 201

Query: 192 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRAPLGS 251
           NW+   +  G +   +L+V++QE+ GWG+   I T  M  ++A F  G P+YR++ P GS
Sbjct: 202 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 261

Query: 252 PLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDK--VHGRLLTHTN 295
           P+T + QV+VA+F + ++  P ++  LYE Q  +      R + HT+
Sbjct: 262 PITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTD 308

BLAST of Carg01070 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 3.8e-56
Identity = 118/293 (40.27%), Postives = 173/293 (59.04%), Query Frame = 0

Query: 10  DDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRVIRQ 69
           +  +   D +VD  +    +  TG WKA  FI+  E  ERL+Y+G+ T+LV YL   + Q
Sbjct: 3   EKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQ 62

Query: 70  DLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTLVPSL 129
              TAA NV  W+G   + PL G F+ADAYLGR+ T+     +Y+ G++LLTLS  VP L
Sbjct: 63  GNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGL 122

Query: 130 KA--CGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQKMS 189
           K   C ++ C  P      +FF A+Y+I++GTGG KP + SFGADQFD++   E+ +K S
Sbjct: 123 KPGNCNADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSS 182

Query: 190 FFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRAPL 249
           FFNW+   +  G +   T++V++Q +VGWG    + T  M  ++  F  G   YR + P 
Sbjct: 183 FFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPG 242

Query: 250 GSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVH---GRLLTHTNRLK 298
           GSPLT + QV+VAAF + ++  P + + L+E  A D+ +    R L HT+ LK
Sbjct: 243 GSPLTRIFQVIVAAFRKISVKVPEDKSLLFET-ADDESNIKGSRKLVHTDNLK 293

BLAST of Carg01070 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 3.8e-56
Identity = 108/279 (38.71%), Postives = 172/279 (61.65%), Query Frame = 0

Query: 6   ENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTR 65
           E  GDD     D +VD +     R+ TG WKA  F++  E  ER++Y+GI+++LVIY+T 
Sbjct: 4   EEVGDD--YTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTT 63

Query: 66  VIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTL 125
            + Q    ++ NV  W G + L P+ G ++ADA+ GR+ T ++S+ +YL G++LLTLS  
Sbjct: 64  KLHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVS 123

Query: 126 VPSLK-----ACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 185
           +P LK         E C++   +   +FF A+Y +++GTGG KP++ + GADQFD+   +
Sbjct: 124 LPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPK 183

Query: 186 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 245
           ++  K SFFNWW   +  G  F  T++VYVQ++VGW +   + T  +A S+ IFL+G  +
Sbjct: 184 DKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRL 243

Query: 246 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEV 280
           YR++ P+GSP T + +V+VA+  +   P  S+S   YE+
Sbjct: 244 YRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYEL 280

BLAST of Carg01070 vs. ExPASy TrEMBL
Match: A0A6J1FT20 (protein NRT1/ PTR FAMILY 5.6-like OS=Cucurbita moschata OX=3662 GN=LOC111447038 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.0e-165
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60
           MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60

Query: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120
           IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL
Sbjct: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120

Query: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180
           TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE
Sbjct: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180

Query: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240

Query: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK
Sbjct: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 297

BLAST of Carg01070 vs. ExPASy TrEMBL
Match: A0A6J1I1F1 (protein NRT1/ PTR FAMILY 5.6-like OS=Cucurbita maxima OX=3661 GN=LOC111470020 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.1e-163
Identity = 292/297 (98.32%), Postives = 294/297 (98.99%), Query Frame = 0

Query: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60
           MKTEAEN GDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKTEAENSGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60

Query: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120
           IYLTRVIRQDLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTVL ST+VYLFGLSLL
Sbjct: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLASTVVYLFGLSLL 120

Query: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180
           TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE
Sbjct: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180

Query: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240

Query: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRL+
Sbjct: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLR 297

BLAST of Carg01070 vs. ExPASy TrEMBL
Match: A0A6J1CW84 (protein NRT1/ PTR FAMILY 5.6-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014970 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.7e-139
Identity = 250/292 (85.62%), Postives = 269/292 (92.12%), Query Frame = 0

Query: 7   NRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRV 66
           N   +  LVHDSSVDHK R+PLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLT+V
Sbjct: 22  NNNHNHDLVHDSSVDHKGRIPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTKV 81

Query: 67  IRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTLV 126
           IRQDLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTVL STIVYL GLSLLT+STLV
Sbjct: 82  IRQDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLASTIVYLLGLSLLTVSTLV 141

Query: 127 PSLKACGSE-PCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQK 186
           PSLKACG+E  CD+PRK+HEVLFFTAIY ISVGTGGHKPSLESFGADQFDDDHAEERKQK
Sbjct: 142 PSLKACGAEITCDQPRKIHEVLFFTAIYFISVGTGGHKPSLESFGADQFDDDHAEERKQK 201

Query: 187 MSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRA 246
           MSFFNWWNSGLCAGVI GVTLIVYVQ+HVGWG++G ILTSVMA SLAIFL+GRPVYR+RA
Sbjct: 202 MSFFNWWNSGLCAGVILGVTLIVYVQDHVGWGVSGAILTSVMAISLAIFLLGRPVYRFRA 261

Query: 247 PLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           PLGSPLTPLLQVLVAAF  RNLPYP + +HLYE+Q+T  + GRLL+HT +LK
Sbjct: 262 PLGSPLTPLLQVLVAAFRNRNLPYPPHPSHLYELQSTHNIQGRLLSHTKKLK 313

BLAST of Carg01070 vs. ExPASy TrEMBL
Match: A0A5A7TPU6 (Protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004790 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 2.0e-137
Identity = 245/297 (82.49%), Postives = 267/297 (89.90%), Query Frame = 0

Query: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60
           MK +  +  DDQI V+DSSVDHK  LPLRASTGVWK+SLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKRDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLV 60

Query: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120
           IYLTRVI +DLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTVL+ST++YL GLSLL
Sbjct: 61  IYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLISTVIYLLGLSLL 120

Query: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180
           T+STLVPSLK C  E C EPRK+HE+LFFTAIYLIS+GTGGHKPSLESFGADQFDDDH E
Sbjct: 121 TMSTLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPE 180

Query: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGM GVILTSVMA SLAIFL+GRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPV 240

Query: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           YRYRAP GSPLTPLLQV++AAF +R L YP +S+ L+EVQ+ DK  GRLL+HT  L+
Sbjct: 241 YRYRAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHEVQSGDKFQGRLLSHTKNLR 297

BLAST of Carg01070 vs. ExPASy TrEMBL
Match: A0A1S3B6K9 (protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo OX=3656 GN=LOC103486351 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 2.0e-137
Identity = 245/297 (82.49%), Postives = 267/297 (89.90%), Query Frame = 0

Query: 1   MKTEAENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLV 60
           MK +  +  DDQI V+DSSVDHK  LPLRASTGVWK+SLFIIAIEFSERLSYFGIATSLV
Sbjct: 10  MKRDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLV 69

Query: 61  IYLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLL 120
           IYLTRVI +DLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTVL+ST++YL GLSLL
Sbjct: 70  IYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLISTVIYLLGLSLL 129

Query: 121 TLSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 180
           T+STLVPSLK C  E C EPRK+HE+LFFTAIYLIS+GTGGHKPSLESFGADQFDDDH E
Sbjct: 130 TMSTLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPE 189

Query: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 240
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGM GVILTSVMA SLAIFL+GRPV
Sbjct: 190 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPV 249

Query: 241 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           YRYRAP GSPLTPLLQV++AAF +R L YP +S+ L+EVQ+ DK  GRLL+HT  L+
Sbjct: 250 YRYRAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHEVQSGDKFQGRLLSHTKNLR 306

BLAST of Carg01070 vs. TAIR 10
Match: AT2G37900.1 (Major facilitator superfamily protein )

HSP 1 Score: 413.3 bits (1061), Expect = 1.6e-115
Identity = 197/288 (68.40%), Postives = 232/288 (80.56%), Query Frame = 0

Query: 10  DDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRVIRQ 69
           D Q  V DSS+D + R+PLRA TG W+A+LFIIAIEFSERLSYFG+AT+LV+YLT ++ Q
Sbjct: 17  DQQKWVLDSSLDSRGRVPLRARTGAWRAALFIIAIEFSERLSYFGLATNLVVYLTTILNQ 76

Query: 70  DLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTLVPSL 129
           DLK A RNVNYW+GVTTLMPL GGF+ADAYLGR++TVLV+T +YL GL LLT+S  +P L
Sbjct: 77  DLKMAIRNVNYWSGVTTLMPLLGGFIADAYLGRYATVLVATTIYLMGLVLLTMSWFIPGL 136

Query: 130 KACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQKMSFF 189
           K C  E C EPRK HEV FF AIYLIS+GTGGHKPSLESFGADQFDDDH EERK KMSFF
Sbjct: 137 KPCHQEVCVEPRKAHEVAFFIAIYLISIGTGGHKPSLESFGADQFDDDHVEERKMKMSFF 196

Query: 190 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRAPLGS 249
           NWWN  LCAG++  VT + Y+++ VGWG+AG+ILT VMA SL IF +G+P YRYR P GS
Sbjct: 197 NWWNVSLCAGILTAVTAVAYIEDRVGWGVAGIILTVVMAISLIIFFIGKPFYRYRTPSGS 256

Query: 250 PLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           PLTP+LQV VAA  +RNLPYPS+ + L+EV  T+   GRLL HT  LK
Sbjct: 257 PLTPILQVFVAAIAKRNLPYPSDPSLLHEVSKTEFTSGRLLCHTEHLK 304

BLAST of Carg01070 vs. TAIR 10
Match: AT3G53960.1 (Major facilitator superfamily protein )

HSP 1 Score: 396.4 bits (1017), Expect = 2.1e-110
Identity = 190/296 (64.19%), Postives = 235/296 (79.39%), Query Frame = 0

Query: 3   TEAENRGDD-QILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVI 62
           TE ++  DD Q  V DSS D +  +PLRA TG W+A+LFII IEFSERLSYFGI+T+LV+
Sbjct: 8   TEPQDSYDDQQKWVLDSSTDSRGEIPLRAQTGAWRAALFIIGIEFSERLSYFGISTNLVV 67

Query: 63  YLTRVIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLT 122
           YLT ++ QDLK A +N NYW+GVTTLMPL GGF+ADAYLGR+ TVL++T +YL GL LLT
Sbjct: 68  YLTTILHQDLKMAVKNTNYWSGVTTLMPLLGGFVADAYLGRYGTVLLATTIYLMGLILLT 127

Query: 123 LSTLVPSLKACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEE 182
           LS  +P LKAC  + C EPRK HE+ FF AIYLIS+GTGGHKPSLESFGADQF+D H EE
Sbjct: 128 LSWFIPGLKACHEDMCVEPRKAHEIAFFIAIYLISIGTGGHKPSLESFGADQFEDGHPEE 187

Query: 183 RKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVY 242
           RK KMS+FNWWN+GLCAG++  VT+IVY+++ +GWG+A +ILT VMATS  IF +G+P Y
Sbjct: 188 RKMKMSYFNWWNAGLCAGILTAVTVIVYIEDRIGWGVASIILTIVMATSFFIFRIGKPFY 247

Query: 243 RYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVHGRLLTHTNRLK 298
           RYRAP GSPLTP+LQV VAA  +RNLP PS+S+ L+E+   +   GRLL+ +  LK
Sbjct: 248 RYRAPSGSPLTPMLQVFVAAIAKRNLPCPSDSSLLHELTNEEYTKGRLLSSSKNLK 303

BLAST of Carg01070 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 223.8 bits (569), Expect = 1.8e-58
Identity = 120/287 (41.81%), Postives = 170/287 (59.23%), Query Frame = 0

Query: 12  QILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRVIRQDL 71
           ++   D SVD     PL+  TG WKA  FI+  E  ERL+Y+GIA +L+ YLT  + Q  
Sbjct: 22  KLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGN 81

Query: 72  KTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTLVPSLK- 131
            +AA NV  W G   L PL G  LADAY GR+ T+   + +Y  G+S LTLS  VP+LK 
Sbjct: 82  VSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKP 141

Query: 132 -ACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQKMSFF 191
             C  + C         +FF  +YLI++GTGG KP + SFGADQFDD  + ER +K SFF
Sbjct: 142 AECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 201

Query: 192 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRAPLGS 251
           NW+   +  G +   +L+V++QE+ GWG+   I T  M  ++A F  G P+YR++ P GS
Sbjct: 202 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 261

Query: 252 PLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDK--VHGRLLTHTN 295
           P+T + QV+VA+F + ++  P ++  LYE Q  +      R + HT+
Sbjct: 262 PITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTD 308

BLAST of Carg01070 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 219.9 bits (559), Expect = 2.7e-57
Identity = 118/293 (40.27%), Postives = 173/293 (59.04%), Query Frame = 0

Query: 10  DDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRVIRQ 69
           +  +   D +VD  +    +  TG WKA  FI+  E  ERL+Y+G+ T+LV YL   + Q
Sbjct: 3   EKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQ 62

Query: 70  DLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTLVPSL 129
              TAA NV  W+G   + PL G F+ADAYLGR+ T+     +Y+ G++LLTLS  VP L
Sbjct: 63  GNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGL 122

Query: 130 KA--CGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQKMS 189
           K   C ++ C  P      +FF A+Y+I++GTGG KP + SFGADQFD++   E+ +K S
Sbjct: 123 KPGNCNADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSS 182

Query: 190 FFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRAPL 249
           FFNW+   +  G +   T++V++Q +VGWG    + T  M  ++  F  G   YR + P 
Sbjct: 183 FFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPG 242

Query: 250 GSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEVQATDKVH---GRLLTHTNRLK 298
           GSPLT + QV+VAAF + ++  P + + L+E  A D+ +    R L HT+ LK
Sbjct: 243 GSPLTRIFQVIVAAFRKISVKVPEDKSLLFET-ADDESNIKGSRKLVHTDNLK 293

BLAST of Carg01070 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 219.9 bits (559), Expect = 2.7e-57
Identity = 108/279 (38.71%), Postives = 172/279 (61.65%), Query Frame = 0

Query: 6   ENRGDDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTR 65
           E  GDD     D +VD +     R+ TG WKA  F++  E  ER++Y+GI+++LVIY+T 
Sbjct: 4   EEVGDD--YTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTT 63

Query: 66  VIRQDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTL 125
            + Q    ++ NV  W G + L P+ G ++ADA+ GR+ T ++S+ +YL G++LLTLS  
Sbjct: 64  KLHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVS 123

Query: 126 VPSLK-----ACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAE 185
           +P LK         E C++   +   +FF A+Y +++GTGG KP++ + GADQFD+   +
Sbjct: 124 LPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPK 183

Query: 186 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPV 245
           ++  K SFFNWW   +  G  F  T++VYVQ++VGW +   + T  +A S+ IFL+G  +
Sbjct: 184 DKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRL 243

Query: 246 YRYRAPLGSPLTPLLQVLVAAFTRRNLPYPSNSAHLYEV 280
           YR++ P+GSP T + +V+VA+  +   P  S+S   YE+
Sbjct: 244 YRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYEL 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6596385.12.1e-165100.00Protein NRT1/ PTR FAMILY 5.6, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7027931.12.1e-165100.00Protein NRT1/ PTR FAMILY 5.6, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022941773.12.1e-165100.00protein NRT1/ PTR FAMILY 5.6-like [Cucurbita moschata][more]
XP_023540060.11.2e-16398.99protein NRT1/ PTR FAMILY 5.6-like [Cucurbita pepo subsp. pepo][more]
XP_022971232.14.4e-16398.32protein NRT1/ PTR FAMILY 5.6-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P0CI032.3e-11468.40Protein NRT1/ PTR FAMILY 5.6 OS=Arabidopsis thaliana OX=3702 GN=NPF5.6 PE=2 SV=1[more]
Q9M3312.9e-10964.19Protein NRT1/ PTR FAMILY 5.7 OS=Arabidopsis thaliana OX=3702 GN=NPF5.7 PE=2 SV=2[more]
P460322.6e-5741.81Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q9M3903.8e-5640.27Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9FNL83.8e-5638.71Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FT201.0e-165100.00protein NRT1/ PTR FAMILY 5.6-like OS=Cucurbita moschata OX=3662 GN=LOC111447038 ... [more]
A0A6J1I1F12.1e-16398.32protein NRT1/ PTR FAMILY 5.6-like OS=Cucurbita maxima OX=3661 GN=LOC111470020 PE... [more]
A0A6J1CW843.7e-13985.62protein NRT1/ PTR FAMILY 5.6-like isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A5A7TPU62.0e-13782.49Protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3B6K92.0e-13782.49protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo OX=3656 GN=LOC103486351 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G37900.11.6e-11568.40Major facilitator superfamily protein [more]
AT3G53960.12.1e-11064.19Major facilitator superfamily protein [more]
AT2G02040.11.8e-5841.81peptide transporter 2 [more]
AT3G54140.12.7e-5740.27peptide transporter 1 [more]
AT5G46040.12.7e-5738.71Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 103..275
e-value: 2.3E-37
score: 128.8
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 18..297
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 7..294
e-value: 4.9E-93
score: 314.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 23..266
NoneNo IPR availablePANTHERPTHR11654:SF459PROTEIN NRT1/ PTR FAMILY 5.6coord: 18..297
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 92..116

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01070-RACarg01070-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity