Carg01059 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01059
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr06: 1418779 .. 1421806 (+)
RNA-Seq Expression	Carg01059
Synteny	Carg01059

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGCGTTCGCTGTTGAAGGTTCCAAGCAATTTACTTTAACCTTAATTCTTTTCGTGTGCTGCGAATCTCTCTGTCTCTCCATTTTACTACAGCACTTAAAAAGGCGAAGCGTTTCTGCAATCTTGGCATCCTTTCTTGCGACCATTTTTGGATTCTTGAAGCTTCTTGGCGTCCGTTTATTTATGAGCATCGAATTTGAATCGGTAATTTCAATCAATTTCTTTACCAAACTTTATATAGTTAGGGTTTATTCGGTAAAGGAGTTCGTGCCATTCGTTCGATTGATCGATTGATGGGATCGACATCTTTGTGCTACAGATGCAATGGGTTCGATATCCTAGATTCGGTCTGTTGTCCTGTTTCCGGTTGAGAATTCGTTGAAGAATTTGGGTTACCGCCATCTCTATCTCCGTTTGATCGTCGATTTCCAGCTATTGCGATGTACCACGACGACGCTTCCGACCCAGACCCTTTCTTCGTTCCGAGATTGCGCCGCAGCGGAAGAAACAGCGAGGATCGCTCCCCTTTCAATCGCATCATTGTTCTACGCAATCCTTCTAATGTTGGTGCTATTAGTAATAATGATATTGGAGGTGGAGGAAATTATGAGATTTATTATGATGATGGTACCGGGGCTAGCCTGCGCCCTTTACCTTATAATATATCCGAGTTCTTGATGGGATCGGGGTTCGATCGTCTTCTCAATCAATTGGCGCAGCTGGAGGTGAACGGGGTTAGCCCATTGGAACAACCACCGGCTTCTAAGGCTGCAATCGAATCGTTGCCTGTGGTTAAGATTCTTGATAGTCATGTGCCTATGGAATCGCATTGTGCGGTCTGTAAGGAGCCATTTGAGCTCGACTCTGAAGCTCGAGAAATGCCGTGTAAACATATTTACCATTCGGATTGCATACTTCCTTGGCTTTCTATCCGGAATTCTTGTCCTGTTTGTCGGCACCAGTTGCCTACCAACAGTGGCAGAAGCTCCCCTGCGTCTGCTGAAGAAGTTGTTGGATTAACAATTTGGAGGCTTCCGGGTGGGGGATTTGCTGTTGGAAGGTTTACTTGGGGTAGGGAAGCTACCCAACATAACCTTCCTGTTGTTTACACTGAAATTGATGGTAGTTTTAGTACCACCAGTGGTGTTCCAAGGAGGATTACATGGGCGTCGAGCCGAGGAAGATCAAACGAAGGTGGTGGTTTTCGTCGTCTTCTACGCAGTTTCTCCTCCTTTTTTGGGAGGTTTAGAAGTTCATCCCGTCGTTCTAGTTCGGGACCTGGTTTAATATGAAGGAGCTTCTCTGCTACATTAATTAATAGACACTCAAGGAGGAATAATCACCCTATGGCGTCCAAGTCCGAGGCGAACTATAAGTTTCCTTCTTAGTTATACTTACAGAGAGGTTGAATTCCCACACCATGTACTCTCTTTAGAAATTGCAAGCGACACGGATTGCATAACGGTCCTTTGGACGATGCGTAGATGTCCAATTTTTGTTTCACTAGGTGTATATAAGCTATACATCAAAAAAGAAATACAGGATTCAATAACTTATATGTAGTTGACTGGAGAAGTTTTTGACACTGTGGGAGACATGTATATGTAGAGAAAGATAGGGAAGGGAGAGGGCTGTACAATCTGCCTGATCAACGGTTTTTGGCAGTTCAAATATTTGGTTGTCTGTTGCTGTTTGAGGTTAGAAGGATGTTTAGTAGCAATGTAACCTTGTAACTTCACTGCAAAGCAGGTCTTATCCTTAAAAAATACGAAAATCTACTGTTTTTAGTATGTCTGTATCGTTACCTTTCATGGTTTCAAGTTAAGATTCATCTGTCAGACATTGTTTAGTGGCATTCATGATTCTTAAGGAACTCTTATTGTCTCTTTAAGCTTACTCTCATTACGTCTTTCTCACAGATTTCATCTCAATCAGGAACTCTTATCCCACCTTTCTGTTTCTGGAGGAGGATCCAACCTCTTTAAGGAAGGCAGATTGACAAAGCTGATAGGGTGGGGATAATTTAATACTCAGGTATGGTCTCTTTTGTATTATTACATTGAAGAACTTTTCATTCAGGGCCATGGGTAGAGATTTTTGTTTATAACAAGTTGATTAATGTGCATTATTATCAAACCCATGCTGGTGTACTCGAATAGGCTCTTGGCTCTAGAAAAAGCTCAAGTATTCATTGGCTTTCTCGTTTGAAACCTTTATGCACATTTGTATGCTGGCCACCCACATGCTTGTTATCTTTTTCTTTTGATATTTGTGTCTGTTTGCCCCAGCCTACATACAACTTAACTATTCCCCTGAGACAATCGTCTAAGCCCACTCTAGTTCACCGATATACTTGATATCATAAATGTAACGTTGGGCTTGGGCTTGGACTTTTACAAATAGTATTAGAGGGTGAATTGTGAGATCTCCACATTGGTTGGAGAGGGGAACGAAACATTCCTTATAAGGGTGTAGAAACCTCTCTCTAGTAGATGCGTTTTAAAACTATGAGTATATCTGCTAGCGATAGGATTTGGTTGTTATAAAAGGTATCAGAGTCAGACATTGAACGGTGTATGGACTTCAGTTTGTTATAATAAAAATGATTTCTTAATGTAAGGATGTCAGTTGAATGCATGTGCAACTTCAGGAGTTTTCTAACTTGTTATCAAAACCAAATGTAGCTTTATTCCAAGTGTCGTTTCCTTTATGCTGCCAATCAGAGGATACCAAAGTGCTTTCTTTGTTGTATGAATTGTCAGCACAACAGCCTGTACTCCTTTTCCATGGGTGATTGCTGGGGAGAATGAATGGAAGTTTATTCGTTTTGTTTGGCATAAAAGAAGTAAGTTCACATGTCTTAGAGAACTTTTTCGAAATTTGTGCTGATTCCTGTCTAGTCATGCCATCAGCTGCTGTTGTAAGCCAAGCTATGATTGTTGGAATTTTGTACTGATATCGTAACGTAAAGGACAGAAGGCTCATCCAGCCAGATCAATATGAACCCAGGAGCTCTGTTTTGTTATTGG

mRNA sequence

GCGCGTTCGCTGTTGAAGGTTCCAAGCAATTTACTTTAACCTTAATTCTTTTCGTGTGCTGCGAATCTCTCTGTCTCTCCATTTTACTACAGCACTTAAAAAGGCGAAGCGTTTCTGCAATCTTGGCATCCTTTCTTGCGACCATTTTTGGATTCTTGAAGCTTCTTGGCGTCCGTTTATTTATGAGCATCGAATTTGAATCGATGCAATGGGTTCGATATCCTAGATTCGAATTTGGGTTACCGCCATCTCTATCTCCGTTTGATCGTCGATTTCCAGCTATTGCGATGTACCACGACGACGCTTCCGACCCAGACCCTTTCTTCGTTCCGAGATTGCGCCGCAGCGGAAGAAACAGCGAGGATCGCTCCCCTTTCAATCGCATCATTGTTCTACGCAATCCTTCTAATGTTGGTGCTATTAGTAATAATGATATTGGAGGTGGAGGAAATTATGAGATTTATTATGATGATGGTACCGGGGCTAGCCTGCGCCCTTTACCTTATAATATATCCGAGTTCTTGATGGGATCGGGGTTCGATCGTCTTCTCAATCAATTGGCGCAGCTGGAGGTGAACGGGGTTAGCCCATTGGAACAACCACCGGCTTCTAAGGCTGCAATCGAATCGTTGCCTGTGGTTAAGATTCTTGATAGTCATCTACCCAACATAACCTTCCTGTTGTTTACACTGAAATTGATGGTAGTTTTAGTACCACCAGTGGTGTTCCAAGGAGGATTACATGGGCGTCGAGCCGAGGAAGATCAAACGAAGGTGGTGGTTTTCGTCGTCTTCTACGCAGTTTCTCCTCCTTTTTTGGGAGGTTTAGAAGTTCATCCCGTCGTTCTAGTTCGGGACCTGTTGACTGGAGAAGTTTTTGACACTGTGGGAGACATCACAACAGCCTGTACTCCTTTTCCATGGGTGATTGCTGGGGAGAATGAATGGAAGTTTATTCGTTTTGTTTGGCATAAAAGAACTGCTGTTGTAAGCCAAGCTATGATTGTTGGAATTTTGTACTGATATCGTAACGTAAAGGACAGAAGGCTCATCCAGCCAGATCAATATGAACCCAGGAGCTCTGTTTTGTTATTGG

Coding sequence (CDS)

ATGAGCATCGAATTTGAATCGATGCAATGGGTTCGATATCCTAGATTCGAATTTGGGTTACCGCCATCTCTATCTCCGTTTGATCGTCGATTTCCAGCTATTGCGATGTACCACGACGACGCTTCCGACCCAGACCCTTTCTTCGTTCCGAGATTGCGCCGCAGCGGAAGAAACAGCGAGGATCGCTCCCCTTTCAATCGCATCATTGTTCTACGCAATCCTTCTAATGTTGGTGCTATTAGTAATAATGATATTGGAGGTGGAGGAAATTATGAGATTTATTATGATGATGGTACCGGGGCTAGCCTGCGCCCTTTACCTTATAATATATCCGAGTTCTTGATGGGATCGGGGTTCGATCGTCTTCTCAATCAATTGGCGCAGCTGGAGGTGAACGGGGTTAGCCCATTGGAACAACCACCGGCTTCTAAGGCTGCAATCGAATCGTTGCCTGTGGTTAAGATTCTTGATAGTCATCTACCCAACATAACCTTCCTGTTGTTTACACTGAAATTGATGGTAGTTTTAGTACCACCAGTGGTGTTCCAAGGAGGATTACATGGGCGTCGAGCCGAGGAAGATCAAACGAAGGTGGTGGTTTTCGTCGTCTTCTACGCAGTTTCTCCTCCTTTTTTGGGAGGTTTAGAAGTTCATCCCGTCGTTCTAGTTCGGGACCTGTTGACTGGAGAAGTTTTTGACACTGTGGGAGACATCACAACAGCCTGTACTCCTTTTCCATGGGTGATTGCTGGGGAGAATGAATGGAAGTTTATTCGTTTTGTTTGGCATAAAAGAACTGCTGTTGTAAGCCAAGCTATGATTGTTGGAATTTTGTACTGA

Protein sequence

MSIEFESMQWVRYPRFEFGLPPSLSPFDRRFPAIAMYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKILDSHLPNITFLLFTLKLMVVLVPPVVFQGGLHGRRAEEDQTKVVVFVVFYAVSPPFLGGLEVHPVVLVRDLLTGEVFDTVGDITTACTPFPWVIAGENEWKFIRFVWHKRTAVVSQAMIVGILY

Homology

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BLAST of Carg01059 vs. NCBI nr
Match: KAG7027942.1 (E3 ubiquitin-protein ligase RDUF1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 567.4 bits (1461), Expect = 6.9e-158
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0


Query: 1   MSIEFESMQWVRYPRFEFGLPPSLSPFDRRFPAIAMYHDDASDPDPFFVPRLRRSGRNSE 60
           MSIEFESMQWVRYPRFEFGLPPSLSPFDRRFPAIAMYHDDASDPDPFFVPRLRRSGRNSE
Sbjct: 1   MSIEFESMQWVRYPRFEFGLPPSLSPFDRRFPAIAMYHDDASDPDPFFVPRLRRSGRNSE 60

Query: 61  DRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFD 120
           DRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFD
Sbjct: 61  DRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFD 120

Query: 121 RLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKILDSHLPNITFLLFTLKLMVVLVPPV 180
           RLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKILDSHLPNITFLLFTLKLMVVLVPPV
Sbjct: 121 RLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKILDSHLPNITFLLFTLKLMVVLVPPV 180

Query: 181 VFQGGLHGRRAEEDQTKVVVFVVFYAVSPPFLGGLEVHPVVLVRDLLTGEVFDTVGDITT 240
           VFQGGLHGRRAEEDQTKVVVFVVFYAVSPPFLGGLEVHPVVLVRDLLTGEVFDTVGDITT
Sbjct: 181 VFQGGLHGRRAEEDQTKVVVFVVFYAVSPPFLGGLEVHPVVLVRDLLTGEVFDTVGDITT 240

Query: 241 ACTPFPWVIAGENEWKFIRFVWHKRTAVVSQAMIVGILY 280
           ACTPFPWVIAGENEWKFIRFVWHKRTAVVSQAMIVGILY
Sbjct: 241 ACTPFPWVIAGENEWKFIRFVWHKRTAVVSQAMIVGILY 279

[Close]

BLAST of Carg01059 vs. NCBI nr
Match: KAG6596400.1 (E3 ubiquitin-protein ligase RDUF2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 327.4 bits (838), Expect = 1.2e-85
Identity = 200/366 (54.64%), Postives = 216/366 (59.02%), Query Frame = 0


Query: 1   MSIEFESMQWVRYPRFEFGLPPSLSPFDRRFPAIAMYHDDASDPDPFFVPRLRRSGRNSE 60
           MSIEFESMQWVRYPRF                AIAMYHDDASDPDPFFVPRLRRSGRNSE
Sbjct: 1   MSIEFESMQWVRYPRF----------------AIAMYHDDASDPDPFFVPRLRRSGRNSE 60

Query: 61  DRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFD 120
           DRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFD
Sbjct: 61  DRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFD 120

Query: 121 RLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKILDSHLPNITFLLFTLKLMVV----- 180
           RLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKILDSH+P  +      +   +     
Sbjct: 121 RLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKILDSHVPMESHCAVCKEPFELDSEAR 180

Query: 181 ------------LVPPVVFQGGL----------HGRRAEEDQTKVVVFVVF------YAV 240
                       ++P +  +              GR +     +VV   ++      +AV
Sbjct: 181 EMPCKHIYHSDCILPWLSIRNSCPVCRHQLPTNSGRSSPASAEEVVGLTIWRLPGGGFAV 240

Query: 241 SPPFLGGLEV--HPVVLVRDLLTGEVFDTVGD---------------------------- 280
              F  G E   H + +V   + G    T G                             
Sbjct: 241 G-RFTWGREATQHNLPVVYTEIDGSFSTTSGVPRRITWASSRGRSNEEKDREGRGLYNLP 300

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BLAST of Carg01059 vs. NCBI nr
Match: XP_023540034.1 (E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo] >XP_023540035.1 E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo] >XP_023540036.1 E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo] >XP_023540037.1 E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo] >XP_023540038.1 E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo] >XP_023540039.1 E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo] >XP_023540041.1 E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 253.8 bits (647), Expect = 1.7e-63
Identity = 124/126 (98.41%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 36  MYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY 95
           MYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY
Sbjct: 1   MYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY 60

Query: 96  DDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI 155
           DDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGV+PLEQPPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVNPLEQPPASKAAIESLPVVKI 120

Query: 156 LDSHLP 162
           LDSH+P
Sbjct: 121 LDSHVP 126

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BLAST of Carg01059 vs. NCBI nr
Match: XP_022971599.1 (E3 ubiquitin-protein ligase RDUF1-like [Cucurbita maxima])

HSP 1 Score: 248.8 bits (634), Expect = 5.4e-62
Identity = 122/126 (96.83%), Postives = 125/126 (99.21%), Query Frame = 0


Query: 36  MYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY 95
           MYHDDAS+P PFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY
Sbjct: 1   MYHDDASEPVPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY 60

Query: 96  DDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI 155
           DDGTGASLRPLPYNISEFLMG+GFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRPLPYNISEFLMGTGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI 120

Query: 156 LDSHLP 162
           LDSH+P
Sbjct: 121 LDSHVP 126

[Close]

BLAST of Carg01059 vs. NCBI nr
Match: KAA0044102.1 (E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK25035.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 232.3 bits (591), Expect = 5.2e-57
Identity = 116/140 (82.86%), Postives = 122/140 (87.14%), Query Frame = 0


Query: 22  PSLSPFDRRFPAIAMYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAIS 81
           PSL P   RFPA AMYHDDAS P+P FVPRLRRSGRN EDRSPFNR IVLR+PSN G +S
Sbjct: 16  PSLYPVVHRFPAAAMYHDDASGPNPSFVPRLRRSGRNGEDRSPFNRFIVLRDPSNSGVVS 75

Query: 82  NNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPP 141
           NN IG GGNYEI+ DDGTGASLRPLP NISEFLMGSGFDRLLNQLA+LEVN VSPLE PP
Sbjct: 76  NNGIGAGGNYEIFCDDGTGASLRPLPSNISEFLMGSGFDRLLNQLAELEVNAVSPLENPP 135

Query: 142 ASKAAIESLPVVKILDSHLP 162
           ASKAAIESLPVVKIL SH+P
Sbjct: 136 ASKAAIESLPVVKILASHVP 155

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BLAST of Carg01059 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.4e-20
Identity = 71/161 (44.10%), Postives = 87/161 (54.04%), Query Frame = 0


Query: 41  ASDPDPFFVPRLRRS---GRNSEDRSPFNRIIVLRNPSNVGAIS--NNDIGGGGN----- 100
           AS P+   V    RS    R S  R+ FN +IVL      GA     N+ G G       
Sbjct: 78  ASTPEVRSVEETHRSIIRRRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGATRERRA 137

Query: 101 YEIYYDDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEV--NGVSPLEQPPASKAAIE 160
           YE YYDDG+G+ LRPLP ++SE LMGSGF+RLL QL+Q+E   NG+     PPASK+AIE
Sbjct: 138 YEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIE 197

Query: 161 SLPVVKILDSHLPNITFLLFTLKLMVVLVPPVVFQGGLHGR 190
           SLP V+I D H         T       V   VF+ G+ GR
Sbjct: 198 SLPRVEISDCH---------TKAEANCAVCTEVFEAGIEGR 229

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BLAST of Carg01059 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.0e-19
Identity = 63/146 (43.15%), Postives = 94/146 (64.38%), Query Frame = 0


Query: 21  PPSLSPFDRRFPAIAMYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAI 80
           PPS S      P+ +M+ D++  P P  V R R       +RSP N +IVLR   +  A 
Sbjct: 62  PPSSS---SSTPSASMHADNS--PTPTIVTRTR------SNRSP-NPVIVLR--GSAAAP 121

Query: 81  SNNDIGGG---GNYEIYYDDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVN---GV 140
           S++ +  G     +++YYDDGT + LRPLP +++EFL+GSGFDRLL+Q++Q+E+N    +
Sbjct: 122 SSDVVSEGLDRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNL 181

Query: 141 SPLEQPPASKAAIESLPVVKILDSHL 161
              E PPASK+AIE+LP+++I  +HL
Sbjct: 182 RSCEHPPASKSAIEALPLIEIDPTHL 193

[Close]

BLAST of Carg01059 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 4.5e-19
Identity = 59/121 (48.76%), Postives = 76/121 (62.81%), Query Frame = 0


Query: 49  VPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGN-------YEIYYDDGTGA 108
           V R RRSGR    R  FN +IVL+     G     + G  G+       +E YYDDG+G+
Sbjct: 82  VIRRRRSGR----RPSFNPVIVLQ-----GGAGEREEGEEGDAARDRRAFEFYYDDGSGS 141

Query: 109 SLRPLPYNISEFLMGSGFDRLLNQLAQLEVN--GVSPLEQPPASKAAIESLPVVKILDSH 161
            LRPLP ++SE LMGSGF+RLL QL+Q+E +  G+     PPASK+AIESLP V+I D H
Sbjct: 142 GLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCH 193

[Close]

BLAST of Carg01059 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.4e-04
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 0


Query: 106 LPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKILDSHL 161
           LP NI ++ +G G ++L+ QLA+ + N       PPASK+AIE+LP+V I  S+L
Sbjct: 166 LPANIGDYFIGPGLEQLIQQLAENDPNRYG---TPPASKSAIEALPLVNITKSNL 217

[Close]

BLAST of Carg01059 vs. ExPASy TrEMBL
Match: A0A6J1I914 (E3 ubiquitin-protein ligase RDUF1-like OS=Cucurbita maxima OX=3661 GN=LOC111470273 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.6e-62
Identity = 122/126 (96.83%), Postives = 125/126 (99.21%), Query Frame = 0


Query: 36  MYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY 95
           MYHDDAS+P PFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY
Sbjct: 1   MYHDDASEPVPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY 60

Query: 96  DDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI 155
           DDGTGASLRPLPYNISEFLMG+GFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRPLPYNISEFLMGTGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI 120

Query: 156 LDSHLP 162
           LDSH+P
Sbjct: 121 LDSHVP 126

[Close]

BLAST of Carg01059 vs. ExPASy TrEMBL
Match: A0A5A7TL92 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001550 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 2.5e-57
Identity = 116/140 (82.86%), Postives = 122/140 (87.14%), Query Frame = 0


Query: 22  PSLSPFDRRFPAIAMYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAIS 81
           PSL P   RFPA AMYHDDAS P+P FVPRLRRSGRN EDRSPFNR IVLR+PSN G +S
Sbjct: 16  PSLYPVVHRFPAAAMYHDDASGPNPSFVPRLRRSGRNGEDRSPFNRFIVLRDPSNSGVVS 75

Query: 82  NNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPP 141
           NN IG GGNYEI+ DDGTGASLRPLP NISEFLMGSGFDRLLNQLA+LEVN VSPLE PP
Sbjct: 76  NNGIGAGGNYEIFCDDGTGASLRPLPSNISEFLMGSGFDRLLNQLAELEVNAVSPLENPP 135

Query: 142 ASKAAIESLPVVKILDSHLP 162
           ASKAAIESLPVVKIL SH+P
Sbjct: 136 ASKAAIESLPVVKILASHVP 155

[Close]

BLAST of Carg01059 vs. ExPASy TrEMBL
Match: A0A6J1CXJ7 (probable E3 ubiquitin-protein ligase RHC2A OS=Momordica charantia OX=3673 GN=LOC111015120 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 2.6e-54
Identity = 110/126 (87.30%), Postives = 115/126 (91.27%), Query Frame = 0


Query: 36  MYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY 95
           MYHDDASDPDP FVPRLRRS RN EDR+PFNRIIVLR+PSN GAISNN IG GGNYEIYY
Sbjct: 1   MYHDDASDPDPSFVPRLRRSRRNGEDRAPFNRIIVLRDPSNAGAISNNGIGAGGNYEIYY 60

Query: 96  DDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI 155
           DDGTG++LRPLP NISEFLMGSGFDRLLNQLAQLEVNGV  LE PPASKAAIESLPVVKI
Sbjct: 61  DDGTGSTLRPLPSNISEFLMGSGFDRLLNQLAQLEVNGVGSLEHPPASKAAIESLPVVKI 120

Query: 156 LDSHLP 162
           L SH+P
Sbjct: 121 LASHVP 126

[Close]

BLAST of Carg01059 vs. ExPASy TrEMBL
Match: A0A1S3B6J2 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103486374 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.9e-52
Identity = 107/126 (84.92%), Postives = 113/126 (89.68%), Query Frame = 0


Query: 36  MYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY 95
           MYHDDAS P+P FVPRLRRSGRN EDRSPFNR IVLR+PSN G +SNN IG GGNYEI+ 
Sbjct: 1   MYHDDASGPNPSFVPRLRRSGRNGEDRSPFNRFIVLRDPSNSGVVSNNGIGAGGNYEIFC 60

Query: 96  DDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI 155
           DDGTGASLRPLP NISEFLMGSGFDRLLNQLA+LEVN VSPLE PPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRPLPSNISEFLMGSGFDRLLNQLAELEVNAVSPLENPPASKAAIESLPVVKI 120

Query: 156 LDSHLP 162
           L SH+P
Sbjct: 121 LASHVP 126

[Close]

BLAST of Carg01059 vs. ExPASy TrEMBL
Match: A0A0A0LFJ3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734090 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.9e-52
Identity = 107/125 (85.60%), Postives = 114/125 (91.20%), Query Frame = 0


Query: 36  MYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGNYEIYY 95
           MYHDDAS P+P FVPRLRRSGRN EDRS FNRIIVLR+PSN G +SNN IGGGGNYEI+ 
Sbjct: 1   MYHDDASGPNPSFVPRLRRSGRNGEDRSSFNRIIVLRDPSNSGVVSNNGIGGGGNYEIFC 60

Query: 96  DDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVNGVSPLEQPPASKAAIESLPVVKI 155
           DDGTGASLRPLP N+SEFLMGSGFDRLLNQLAQLEVNGVSPLE PPASKAAIESLPVVKI
Sbjct: 61  DDGTGASLRPLPSNVSEFLMGSGFDRLLNQLAQLEVNGVSPLENPPASKAAIESLPVVKI 120

Query: 156 LDSHL 161
           L +H+
Sbjct: 121 LANHV 125

[Close]

BLAST of Carg01059 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.0e-21
Identity = 71/161 (44.10%), Postives = 87/161 (54.04%), Query Frame = 0


Query: 41  ASDPDPFFVPRLRRS---GRNSEDRSPFNRIIVLRNPSNVGAIS--NNDIGGGGN----- 100
           AS P+   V    RS    R S  R+ FN +IVL      GA     N+ G G       
Sbjct: 78  ASTPEVRSVEETHRSIIRRRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGATRERRA 137

Query: 101 YEIYYDDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEV--NGVSPLEQPPASKAAIE 160
           YE YYDDG+G+ LRPLP ++SE LMGSGF+RLL QL+Q+E   NG+     PPASK+AIE
Sbjct: 138 YEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIE 197

Query: 161 SLPVVKILDSHLPNITFLLFTLKLMVVLVPPVVFQGGLHGR 190
           SLP V+I D H         T       V   VF+ G+ GR
Sbjct: 198 SLPRVEISDCH---------TKAEANCAVCTEVFEAGIEGR 229

[Close]

BLAST of Carg01059 vs. TAIR 10
Match: AT2G39720.1 (RING-H2 finger C2A )

HSP 1 Score: 97.8 bits (242), Expect = 1.4e-20
Identity = 63/146 (43.15%), Postives = 94/146 (64.38%), Query Frame = 0


Query: 21  PPSLSPFDRRFPAIAMYHDDASDPDPFFVPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAI 80
           PPS S      P+ +M+ D++  P P  V R R       +RSP N +IVLR   +  A 
Sbjct: 62  PPSSS---SSTPSASMHADNS--PTPTIVTRTR------SNRSP-NPVIVLR--GSAAAP 121

Query: 81  SNNDIGGG---GNYEIYYDDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVN---GV 140
           S++ +  G     +++YYDDGT + LRPLP +++EFL+GSGFDRLL+Q++Q+E+N    +
Sbjct: 122 SSDVVSEGLDRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNL 181

Query: 141 SPLEQPPASKAAIESLPVVKILDSHL 161
              E PPASK+AIE+LP+++I  +HL
Sbjct: 182 RSCEHPPASKSAIEALPLIEIDPTHL 193

[Close]

BLAST of Carg01059 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 96.7 bits (239), Expect = 3.2e-20
Identity = 59/121 (48.76%), Postives = 76/121 (62.81%), Query Frame = 0


Query: 49  VPRLRRSGRNSEDRSPFNRIIVLRNPSNVGAISNNDIGGGGN-------YEIYYDDGTGA 108
           V R RRSGR    R  FN +IVL+     G     + G  G+       +E YYDDG+G+
Sbjct: 82  VIRRRRSGR----RPSFNPVIVLQ-----GGAGEREEGEEGDAARDRRAFEFYYDDGSGS 141

Query: 109 SLRPLPYNISEFLMGSGFDRLLNQLAQLEVN--GVSPLEQPPASKAAIESLPVVKILDSH 161
            LRPLP ++SE LMGSGF+RLL QL+Q+E +  G+     PPASK+AIESLP V+I D H
Sbjct: 142 GLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCH 193

[Close]

BLAST of Carg01059 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 3.2e-04
Identity = 37/143 (25.87%), Postives = 61/143 (42.66%), Query Frame = 0


Query: 22  PSLSPFDRRFPAIAMYHDDASDPD---------PFFVPRLRRSGRNSEDRSPFNRIIVLR 81
           P   PF    P I  +   A+ P          P   P+ R + +N +    F+    L+
Sbjct: 74  PMADPFSTLLPLI--FGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQS-DAFDPFTFLQ 133

Query: 82  NPSNVGAISNNDIGGGGNYEIYYDDGTGASLRPLPYNISEFLMGSGFDRLLNQLAQLEVN 141
           N       S      G ++E   ++        +P N  ++  G G ++L+ QLA+ + N
Sbjct: 134 NHLQTLRSS------GTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPN 193

Query: 142 GVSPLEQPPASKAAIESLPVVKI 156
                  PPASK+AI++LP VK+
Sbjct: 194 RYG---TPPASKSAIDALPTVKV 204

The following BLAST results are available for this feature:

BLAST of Carg01059 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027942.1	6.9e-158	100.00	E3 ubiquitin-protein ligase RDUF1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6596400.1	1.2e-85	54.64	E3 ubiquitin-protein ligase RDUF2, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023540034.1	1.7e-63	98.41	E3 ubiquitin-protein ligase RDUF1-like [Cucurbita pepo subsp. pepo] >XP_02354003...	[more]
XP_022971599.1	5.4e-62	96.83	E3 ubiquitin-protein ligase RDUF1-like [Cucurbita maxima]	[more]
KAA0044102.1	5.2e-57	82.86	E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK25035.1 E3...	[more]

BLAST of Carg01059 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SNB6	1.4e-20	44.10	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
O22283	2.0e-19	43.15	Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q940T5	4.5e-19	48.76	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
P0CH30	1.4e-04	45.45	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]

BLAST of Carg01059 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I914	2.6e-62	96.83	E3 ubiquitin-protein ligase RDUF1-like OS=Cucurbita maxima OX=3661 GN=LOC1114702...	[more]
A0A5A7TL92	2.5e-57	82.86	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1CXJ7	2.6e-54	87.30	probable E3 ubiquitin-protein ligase RHC2A OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A1S3B6J2	1.9e-52	84.92	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103486374 P...	[more]
A0A0A0LFJ3	1.9e-52	85.60	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734090 P...	[more]

BLAST of Carg01059 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46620.1	1.0e-21	44.10	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G39720.1	1.4e-20	43.15	RING-H2 finger C2A	[more]
AT5G59550.1	3.2e-20	48.76	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G19950.1	3.2e-04	25.87	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR15710:SF61	E3 UBIQUITIN-PROTEIN LIGASE RHC2A-RELATED	coord: 27..160
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 27..160

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01059-RA	Carg01059-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity