Carg01047 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01047
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCdk-activating kinase assembly factor
LocationCarg_Chr06: 1485683 .. 1488582 (-)
RNA-Seq ExpressionCarg01047
SyntenyCarg01047
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGCCAAAATCCAGAGAAAAGAAAAAGAAAAAGTATCTTCTTAAAGCCCACACGACGAAGTTGCCCGCTGCTATGGCGGGCAGGTTCAAATTACGAGGCCATTTTAATTCAATTCATTCATTCATTCATTCATTCATTCACTATCACATGCTCTCTTCCTTCTCATCCACGCCTGCAATTCCCCCACTCATCTCCATCTCCTTGTACGTCCGCCATTGAAGCCTGGTGAGCGAGCTTCGTCTCGAGCTCCTCTCCATGTTCTAGGAGGCCATTTATTTAACTGGAGGTCGCAGAGTGAGGGGGGAATGGGCCAGGGACTGAGCTGCGCGGACCGGCATGGAACTGGAGTCTTCTTCAGAGCTCTGCAGAACGGAGATGTGGAAGTTGTTCGAGCCTTGATGGAGATTGATTCCTCCATCTTACACCAAACAACCAAACATCAAGGACTATCTGCTCTACATGTAGCGGCTGCCAATGGACAGACCGATGTGAGTTTGAGTTGAAGTTCAAGAGATTTCCCTTGGTTTTTATTCATTGAGTATTTTTTGATGTAGATTCTTTCGATGCTCTTGGACTGGAATGTGAATCCGGACGTTTTGAATCGGCATAATCAGGTTTTTGTTTCGTTTGATCGACTGTGTGTGTTCAGCTCCTTGGCAGTTCGATTCATCTCTCGATTGAACTGATCCTTGTTTCGGTTGATTTAGACTCCTCTGATGCTGGCTGCGATGAGCGGGAAGATAGCTTGTGTTCAGAGGCTGATCGAAGCAGGAGCTAATGTGCGTTTTTTCAGAAGTTTGTATCTAATTTTGATGCAATTTTGCTTACCGGTGTTTGTTTGACATCCATAGATATTGATGTTTGATTCTACACATCGAAGAACCTGCTTGCATTATGCAGCTTACTATGGGCATTCTGATTGCCTGCAAGCGATTCTCTCTGCTGCGCATACCACCCCTGTATCCGACTCTTGGTTAGTCAATTTACTCTTCTTCTTCTTCAAGCATTTACCTTCGAGTATTGGCTTAATATGATGGGTTTTGAATTTGAATTGAATGGAAATGGCCAGGGGGTTTGCAAGATTTGTGAACATTAGGGATGGGGATGGCTCAACTCCATTGCATTTATCCGCCAGGCAGAGCCAACCTGAGTGTGTCCGTATCCTTCTTAGCAATGGTGCTCTTGTCTGTGTCTCCACTTGCAGGTATGAGGAATGATCTTTCATTCTTTCATTCTGAGTATATACAGAGAATGATGGTTTGTTGTGAATATGTTATGCAGTTGTCCAGGTAGTTCGCCACTCCATTTAGCTGCTCGTGGTGGTTCCTTGGAATGTGTTCGAGAATTGCTGGCTTGGGGAGCTGACAGATTTCAGTTCGACTCATATGGGTAAGCTGAAAAAAAAAAAAGAAAAAAGAAAACTAGAGTTATAACCATGCTTTGCTTAAAACAGGCACAATTTAGAAGTAAACAATGGAACTCCTAATGAAGTTTTCTCAAGGTATCTGCTTGACCATTTGCAGGAGGATACCATTTACCGTTGCTTTAAAGCATAAGCATCAAGCTTGTGCAGCTTTGTTGAACCCTTCATCACCAGAACCTCTCGTCTGGCCTTCACCATTGAAGTTAATCATTGAGCTACATCCAGATGCAAAAGTTTTGCTAGAAAAGGCCTTGATGGATGCAAACATGGAGAGAGAGAAGGCCATATTTAAGGAAACATTCGTCACACCTCCTTGTCCTTTCCAATCTGATTTGGACATTGATTCTGATTTAGACAATGATTTAGCATCTGAGGTAATTCAAACACACTTCATTTTGGGTTTTTTGTCGAATAATTTCAAGTCAGGTGTCAATACATGAACACAATGAATCTGGAAAGGATTTTTTAACAGCCTAAGCTCACCGCTAGCAGAAATTGTCCTCTTTGGACTTTTCCTTCTGAGCTTTCCCTTAAGGTTGTAAGCATCCGTTAAGGAGAAGTTTACACACTCTTAAAAGGAGTGCTTCATTCTCCTCTTGAATCGATGTGGAATCTCACAATCCATCCTTTCGGGGCCAGTGTCCCCGCTGGCACACCGCTCTATGCTTGGCTCTTATACTATTTGTAATAGCCCAAACCCACCTTTAGCAGATATTGTCGTCTTTCAGCTTTTTCTTTGGGCTTCCCCTCAAGGTTTTAAAACTCATCCGTTAAAAAGAGATTTCCACCCCTTATAAGAAATGCTTCGTTCCCCTCAACATCCGAGGTGGGATCTCACATATTCCCTCTCAATGTTCTAGTTTTACTTCTTGGGATACTGCTCTGTTGCTTATATTTCGATTTCTGTAATTAATTTGGTCAAGACTATAGCTCATAACTATGTCCGCAACATTCTTACATCACTCGTTGATTCTATCATACGAACAGGCTAGCGATACAGAGTTGTGCAGCATATGCTTTGAACAGATGTGCAATATTGAGGCTCAACCATGTGGCCATAGAATGTGTGCCCATTGCACATTATCTTTATGCTGCCATAAGAAGCCTAATCCAACTACAGCTTGTCCAACAGCCCCTGTTTGCCCCTTTTGCAGAGGCAGCATCACCCAGCTTCTCGTTGCTGAGGTGAAGAGTCATGACAAGGTAGACCAAGAGATTTCCCCCTTGAAGCTGAAGGGGTTGACAACGTCAAATTTCAATGAAGAAAACAGCAGCTTGAAGAGCTTATCTATCCTATTTGGGAAGTTGAGTGAACAAAATTCAGGGACATGTGTTAAGTCTTAAAAAAGGCCTTACCAGATGGATAATCCAGCTGATATGGCAACCTCAGATGTCCTTCCATCTGGGTATATAATTTTTACTTCACCTATAAAGCATCTATATTTGGATGTACTGAGGCTGGCCACAAATCTAA

mRNA sequence

ATGACGCCAAAATCCAGAGAAAAGAAAAAGAAAAAGTATCTTCTTAAAGCCCACACGACGAAGTTGCCCGCTGCTATGGCGGGCAGGTCGCAGAGTGAGGGGGGAATGGGCCAGGGACTGAGCTGCGCGGACCGGCATGGAACTGGAGTCTTCTTCAGAGCTCTGCAGAACGGAGATGTGGAAGTTGTTCGAGCCTTGATGGAGATTGATTCCTCCATCTTACACCAAACAACCAAACATCAAGGACTATCTGCTCTACATGTAGCGGCTGCCAATGGACAGACCGATATTCTTTCGATGCTCTTGGACTGGAATGTGAATCCGGACGTTTTGAATCGGCATAATCAGACTCCTCTGATGCTGGCTGCGATGAGCGGGAAGATAGCTTGTGTTCAGAGGCTGATCGAAGCAGGAGCTAATATATTGATGTTTGATTCTACACATCGAAGAACCTGCTTGCATTATGCAGCTTACTATGGGCATTCTGATTGCCTGCAAGCGATTCTCTCTGCTGCGCATACCACCCCTGTATCCGACTCTTGGGGGTTTGCAAGATTTGTGAACATTAGGGATGGGGATGGCTCAACTCCATTGCATTTATCCGCCAGGCAGAGCCAACCTGAGTGTGTCCGTATCCTTCTTAGCAATGGTGCTCTTGTCTGTGTCTCCACTTGCAGTTGTCCAGGTAGTTCGCCACTCCATTTAGCTGCTCGTGGTGGTTCCTTGGAATGTGTTCGAGAATTGCTGGCTTGGGGAGCTGACAGATTTCAGTTCGACTCATATGGGAGGATACCATTTACCGTTGCTTTAAAGCATAAGCATCAAGCTTGTGCAGCTTTGTTGAACCCTTCATCACCAGAACCTCTCGTCTGGCCTTCACCATTGAAGTTAATCATTGAGCTACATCCAGATGCAAAAGTTTTGCTAGAAAAGGCCTTGATGGATGCAAACATGGAGAGAGAGAAGGCCATATTTAAGGAAACATTCGTCACACCTCCTTGTCCTTTCCAATCTGATTTGGACATTGATTCTGATTTAGACAATGATTTAGCATCTGAGGCTAGCGATACAGAGTTGTGCAGCATATGCTTTGAACAGATGTGCAATATTGAGGCTCAACCATGTGGCCATAGAATGTGTGCCCATTGCACATTATCTTTATGCTGCCATAAGAAGCCTAATCCAACTACAGCTTGTCCAACAGCCCCTGTTTGCCCCTTTTGCAGAGGCAGCATCACCCAGCTTCTCGTTGCTGAGGTGAAGAGTCATGACAAGGTAGACCAAGAGATTTCCCCCTTGAAGCTGAAGGGGTTGACAACGTCAAATTTCAATGAAGAAAACAGCAGCTTGAAGAGCTTATCTATCCTATTTGGGAAGTTGAGTGAACAAAATTCAGGGACATGTGTTAAGTCTTAAAAAAGGCCTTACCAGATGGATAATCCAGCTGATATGGCAACCTCAGATGTCCTTCCATCTGGGTATATAATTTTTACTTCACCTATAAAGCATCTATATTTGGATGTACTGAGGCTGGCCACAAATCTAA

Coding sequence (CDS)

ATGACGCCAAAATCCAGAGAAAAGAAAAAGAAAAAGTATCTTCTTAAAGCCCACACGACGAAGTTGCCCGCTGCTATGGCGGGCAGGTCGCAGAGTGAGGGGGGAATGGGCCAGGGACTGAGCTGCGCGGACCGGCATGGAACTGGAGTCTTCTTCAGAGCTCTGCAGAACGGAGATGTGGAAGTTGTTCGAGCCTTGATGGAGATTGATTCCTCCATCTTACACCAAACAACCAAACATCAAGGACTATCTGCTCTACATGTAGCGGCTGCCAATGGACAGACCGATATTCTTTCGATGCTCTTGGACTGGAATGTGAATCCGGACGTTTTGAATCGGCATAATCAGACTCCTCTGATGCTGGCTGCGATGAGCGGGAAGATAGCTTGTGTTCAGAGGCTGATCGAAGCAGGAGCTAATATATTGATGTTTGATTCTACACATCGAAGAACCTGCTTGCATTATGCAGCTTACTATGGGCATTCTGATTGCCTGCAAGCGATTCTCTCTGCTGCGCATACCACCCCTGTATCCGACTCTTGGGGGTTTGCAAGATTTGTGAACATTAGGGATGGGGATGGCTCAACTCCATTGCATTTATCCGCCAGGCAGAGCCAACCTGAGTGTGTCCGTATCCTTCTTAGCAATGGTGCTCTTGTCTGTGTCTCCACTTGCAGTTGTCCAGGTAGTTCGCCACTCCATTTAGCTGCTCGTGGTGGTTCCTTGGAATGTGTTCGAGAATTGCTGGCTTGGGGAGCTGACAGATTTCAGTTCGACTCATATGGGAGGATACCATTTACCGTTGCTTTAAAGCATAAGCATCAAGCTTGTGCAGCTTTGTTGAACCCTTCATCACCAGAACCTCTCGTCTGGCCTTCACCATTGAAGTTAATCATTGAGCTACATCCAGATGCAAAAGTTTTGCTAGAAAAGGCCTTGATGGATGCAAACATGGAGAGAGAGAAGGCCATATTTAAGGAAACATTCGTCACACCTCCTTGTCCTTTCCAATCTGATTTGGACATTGATTCTGATTTAGACAATGATTTAGCATCTGAGGCTAGCGATACAGAGTTGTGCAGCATATGCTTTGAACAGATGTGCAATATTGAGGCTCAACCATGTGGCCATAGAATGTGTGCCCATTGCACATTATCTTTATGCTGCCATAAGAAGCCTAATCCAACTACAGCTTGTCCAACAGCCCCTGTTTGCCCCTTTTGCAGAGGCAGCATCACCCAGCTTCTCGTTGCTGAGGTGAAGAGTCATGACAAGGTAGACCAAGAGATTTCCCCCTTGAAGCTGAAGGGGTTGACAACGTCAAATTTCAATGAAGAAAACAGCAGCTTGAAGAGCTTATCTATCCTATTTGGGAAGTTGAGTGAACAAAATTCAGGGACATGTGTTAAGTCTTAA

Protein sequence

MTPKSREKKKKKYLLKAHTTKLPAAMAGRSQSEGGMGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSNGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCPFQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNPTTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSILFGKLSEQNSGTCVKS
Homology
BLAST of Carg01047 vs. NCBI nr
Match: KAG7027954.1 (putative E3 ubiquitin-protein ligase XBAT31, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 953.7 bits (2464), Expect = 5.8e-274
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MTPKSREKKKKKYLLKAHTTKLPAAMAGRSQSEGGMGQGLSCADRHGTGVFFRALQNGDV 60
           MTPKSREKKKKKYLLKAHTTKLPAAMAGRSQSEGGMGQGLSCADRHGTGVFFRALQNGDV
Sbjct: 1   MTPKSREKKKKKYLLKAHTTKLPAAMAGRSQSEGGMGQGLSCADRHGTGVFFRALQNGDV 60

Query: 61  EVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNRHNQTPLM 120
           EVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNRHNQTPLM
Sbjct: 61  EVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNRHNQTPLM 120

Query: 121 LAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAILSAAHTTPVSDS 180
           LAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAILSAAHTTPVSDS
Sbjct: 121 LAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAILSAAHTTPVSDS 180

Query: 181 WGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSNGALVCVSTCSCPGSSPLHLAARGG 240
           WGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSNGALVCVSTCSCPGSSPLHLAARGG
Sbjct: 181 WGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSNGALVCVSTCSCPGSSPLHLAARGG 240

Query: 241 SLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQACAALLNPSSPEPLVWPSPLKLIIE 300
           SLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQACAALLNPSSPEPLVWPSPLKLIIE
Sbjct: 241 SLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQACAALLNPSSPEPLVWPSPLKLIIE 300

Query: 301 LHPDAKVLLEKALMDANMEREKAIFKETFVTPPCPFQSDLDIDSDLDNDLASEASDTELC 360
           LHPDAKVLLEKALMDANMEREKAIFKETFVTPPCPFQSDLDIDSDLDNDLASEASDTELC
Sbjct: 301 LHPDAKVLLEKALMDANMEREKAIFKETFVTPPCPFQSDLDIDSDLDNDLASEASDTELC 360

Query: 361 SICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNPTTACPTAPVCPFCRGSITQLLVAEV 420
           SICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNPTTACPTAPVCPFCRGSITQLLVAEV
Sbjct: 361 SICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNPTTACPTAPVCPFCRGSITQLLVAEV 420

Query: 421 KSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSILFGKLSEQNSGTCVKS 472
           KSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSILFGKLSEQNSGTCVKS
Sbjct: 421 KSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSILFGKLSEQNSGTCVKS 471

BLAST of Carg01047 vs. NCBI nr
Match: KAG6596410.1 (putative E3 ubiquitin-protein ligase XBAT31, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 922.2 bits (2382), Expect = 1.9e-264
Identity = 470/536 (87.69%), Postives = 470/536 (87.69%), Query Frame = 0

Query: 1   MTPKSREKKKKKYLLKAHTTKLPAAMAG-------------------------------- 60
           MTPKSREKKKKKYLLKAHTTKLPAAMAG                                
Sbjct: 1   MTPKSREKKKKKYLLKAHTTKLPAAMAGRFKLRGHFNSIHSFIHSLSHALFLLIHACNSP 60

Query: 61  ---------------------------------RSQSEGGMGQGLSCADRHGTGVFFRAL 120
                                            R QSEGGMGQGLSCADRHGTGVFFRAL
Sbjct: 61  THLHLLVRPPLKPGERASSRGPLHVLGGHLFNWRLQSEGGMGQGLSCADRHGTGVFFRAL 120

Query: 121 QNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNRHN 180
           QNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNRHN
Sbjct: 121 QNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNRHN 180

Query: 181 QTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAILSAAHTT 240
           QTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAILSAAHTT
Sbjct: 181 QTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAILSAAHTT 240

Query: 241 PVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSNGALVCVSTCSCPGSSPLHL 300
           PVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSNGALVCVSTCSCPGSSPLHL
Sbjct: 241 PVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSNGALVCVSTCSCPGSSPLHL 300

Query: 301 AARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQACAALLNPSSPEPLVWPSPL 360
           AARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQACAALLNPSSPEPLVWPSPL
Sbjct: 301 AARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQACAALLNPSSPEPLVWPSPL 360

Query: 361 KLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCPFQSDLDIDSDLDNDLASEAS 420
           KLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCPFQSDLDIDSDLDNDLASEAS
Sbjct: 361 KLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCPFQSDLDIDSDLDNDLASEAS 420

Query: 421 DTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNPTTACPTAPVCPFCRGSITQL 472
           DTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNPTTACPTAPVCPFCRGSITQL
Sbjct: 421 DTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNPTTACPTAPVCPFCRGSITQL 480

BLAST of Carg01047 vs. NCBI nr
Match: XP_023540882.1 (putative E3 ubiquitin-protein ligase XBAT31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 890.2 bits (2299), Expect = 7.8e-255
Identity = 436/436 (100.00%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT
Sbjct: 1   MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY
Sbjct: 61  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS
Sbjct: 121 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 180

Query: 216 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 275
           NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 276 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 335
           ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP
Sbjct: 241 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 300

Query: 336 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 395
           FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 396 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 455
           TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI
Sbjct: 361 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 420

Query: 456 LFGKLSEQNSGTCVKS 472
           LFGKLSEQNSGTCVKS
Sbjct: 421 LFGKLSEQNSGTCVKS 436

BLAST of Carg01047 vs. NCBI nr
Match: XP_022971571.1 (putative E3 ubiquitin-protein ligase XBAT31 [Cucurbita maxima])

HSP 1 Score: 888.6 bits (2295), Expect = 2.3e-254
Identity = 435/436 (99.77%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT
Sbjct: 1   MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY
Sbjct: 61  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS
Sbjct: 121 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 180

Query: 216 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 275
           NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 276 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 335
           ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP
Sbjct: 241 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 300

Query: 336 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 395
           FQSDLDIDSDLDNDLASEASDTELCSICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 FQSDLDIDSDLDNDLASEASDTELCSICFEQICNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 396 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 455
           TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI
Sbjct: 361 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 420

Query: 456 LFGKLSEQNSGTCVKS 472
           LFGKLSEQNSGTCVKS
Sbjct: 421 LFGKLSEQNSGTCVKS 436

BLAST of Carg01047 vs. NCBI nr
Match: XP_022948919.1 (putative E3 ubiquitin-protein ligase XBAT31 [Cucurbita moschata])

HSP 1 Score: 887.9 bits (2293), Expect = 3.9e-254
Identity = 434/436 (99.54%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT
Sbjct: 1   MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY
Sbjct: 61  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS
Sbjct: 121 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 180

Query: 216 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 275
           NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQ+DSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQYDSYGRIPFTVALKHKHQ 240

Query: 276 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 335
           ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP
Sbjct: 241 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 300

Query: 336 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 395
           FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 396 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 455
           TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEEN+SLKSLSI
Sbjct: 361 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENTSLKSLSI 420

Query: 456 LFGKLSEQNSGTCVKS 472
           LFGKLSEQNSGTCVKS
Sbjct: 421 LFGKLSEQNSGTCVKS 436

BLAST of Carg01047 vs. ExPASy Swiss-Prot
Match: Q94B55 (Putative E3 ubiquitin-protein ligase XBAT31 OS=Arabidopsis thaliana OX=3702 GN=XBAT31 PE=2 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.1e-141
Identity = 262/450 (58.22%), Postives = 328/450 (72.89%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQ +SC  R   G+ F ++Q GD+  +R +M  + S+L+QTT +   S LHVAAANGQ 
Sbjct: 1   MGQSMSCGSRPEHGI-FASVQCGDIITIRRVMATEPSLLNQTTPYDRHSVLHVAAANGQI 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           +ILS+LL+   NPD+LNRH QTPLMLAAM G+I+CV++L E GANILMFDS +RRTCLHY
Sbjct: 61  EILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AAYYGH++C+QAILSAA ++PV+  WG+ARFVNIRD  G+TPLHL+ARQ +PECV +LL 
Sbjct: 121 AAYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLLD 180

Query: 216 NGALVCVSTC--SCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHK 275
           +G+LVC ST     PGS+PLHLAAR GS++CVR+LLAWGADR Q D+ GRIP+ VA+KHK
Sbjct: 181 SGSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKHK 240

Query: 276 HQACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPP 335
           H AC ALLNPSS EPLVWPSPLK I EL+ +AK+LLE+ALM+AN EREK I K T  + P
Sbjct: 241 HGACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSLP 300

Query: 336 CPFQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKP 395
            P  SD D       D  SE SDTELC ICFEQ+C IE + CGH+MCA CTL+LCCH KP
Sbjct: 301 SPSFSDTD-------DNMSEVSDTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNKP 360

Query: 396 NPTTACPTAPVCPFCRGSITQLLVAEVKSHDK-----------VDQE---ISPLKL-KGL 455
           NPTT+  T PVCPFCR +I  L+VA+  +++            VD+E   +S  K  K  
Sbjct: 361 NPTTSTVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKHR 420

Query: 456 TTSNFNEENSSLKSLSIL--FGKLSEQNSG 467
            + N  EE+SS   LS +  FG+++ + SG
Sbjct: 421 RSINLGEESSSFMGLSTIGSFGRITGRGSG 442

BLAST of Carg01047 vs. ExPASy Swiss-Prot
Match: Q65XV2 (E3 ubiquitin-protein ligase XB3 OS=Oryza sativa subsp. japonica OX=39947 GN=XB3 PE=1 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.0e-140
Identity = 253/436 (58.03%), Postives = 310/436 (71.10%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MG G+SCA       FFRA Q GD++ + AL+  D S+  + T +  LS LH+AAANG+ 
Sbjct: 1   MGHGVSCARTGDEHDFFRAAQLGDLDALAALLAADPSLARRATLYDRLSVLHIAAANGRI 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           ++LSM LD    PD +NRH QTPLMLAAM GKI CV +L++A ANILMFDS H RTCLH+
Sbjct: 61  EVLSMFLDRGAPPDAVNRHKQTPLMLAAMHGKIDCVLKLLQADANILMFDSVHARTCLHH 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AAYYGH DCLQAIL+AA TTPV+DSWGFARFVN+RD  G+TPLHL+ARQ +P CV++LL 
Sbjct: 121 AAYYGHVDCLQAILAAAQTTPVADSWGFARFVNVRDDHGATPLHLAARQGRPGCVQVLLE 180

Query: 216 NGALVCVSTCS--CPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHK 275
           NGA+V   T S   PGS+ LHLAAR G+L+C+R+LLAWGADR Q DS GRIP++VALK  
Sbjct: 181 NGAIVSALTGSYGFPGSTSLHLAARSGNLDCIRKLLAWGADRLQRDSAGRIPYSVALKRN 240

Query: 276 HQACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPP 335
           H ACAALLNP+S EP+VWPSPLK I EL P+AK LLE ALM+AN EREK I   T  + P
Sbjct: 241 HGACAALLNPTSAEPMVWPSPLKFISELEPEAKALLEAALMEANREREKKILNGTKYSLP 300

Query: 336 CPFQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKP 395
            P       D   D+D  SE SDTELC ICF+Q C IE Q CGH+MCA CTL+LCCH KP
Sbjct: 301 SPSPG----DDSADDDACSEVSDTELCCICFDQACTIEVQDCGHQMCAPCTLALCCHNKP 360

Query: 396 NPTTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQ--EISPLKLKGLTTSNFNEENSSLK 455
           NPTT  P +P CPFCRGSI++L+VA+ +S    D+   +   + +   + N +E +SS K
Sbjct: 361 NPTTLTPPSPACPFCRGSISRLVVAQTRSACDPDKPSSLQLTRKRSRRSHNLSEGSSSFK 420

Query: 456 SLSILFGKLSEQNSGT 468
            L    G  S+   G+
Sbjct: 421 GLPSAMGSFSKLGRGS 432

BLAST of Carg01047 vs. ExPASy Swiss-Prot
Match: Q94CT7 (Probable E3 ubiquitin-protein ligase XBOS31 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS31 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.2e-114
Identity = 214/392 (54.59%), Postives = 270/392 (68.88%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MG GLSC+        FRA Q GD+  + AL+  D ++  + T +   +ALH+AAANG+ 
Sbjct: 1   MGHGLSCSRDTDEYDLFRAAQLGDIHALSALLAADPALARRATVYDRFTALHIAAANGRL 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
            +LSMLLD + + DVL+R  QTPLM+AAM G   CV RL+  GAN+L FDS   RTCLH+
Sbjct: 61  QVLSMLLDRDGDVDVLSRKKQTPLMVAAMRGNTECVVRLLRGGANVLTFDSPRARTCLHH 120

Query: 156 AAYYGHSDCLQAILSAAHTT--PVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRIL 215
           AAYYGH++CLQAIL AA     PV+ SWGFARFVN+RD  G+TPLHL+AR ++  CVR+L
Sbjct: 121 AAYYGHAECLQAILGAAAQAQGPVAASWGFARFVNVRDERGATPLHLAARHARASCVRLL 180

Query: 216 LSNGALVCVSTC--SCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALK 275
           L  GA+V   T     PGS+ LHLAAR GS+EC+RELLAWGADR Q DS GRI + VA++
Sbjct: 181 LDKGAIVSAPTAVYGFPGSTALHLAARAGSMECIRELLAWGADRLQRDSAGRIAYAVAMR 240

Query: 276 HKHQACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVT 335
             H+ACAALLNP++ EP+VWPSPLK I EL  DAK LLE ALM+AN EREK I       
Sbjct: 241 RGHRACAALLNPAAAEPIVWPSPLKFIGELEADAKALLEAALMEANREREKRIL------ 300

Query: 336 PPCPFQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHK 395
                 SD++I    D +  SE  + E C+ICFEQ C++E + CGH+MCA CTL++CCH 
Sbjct: 301 ----HGSDINIKGG-DEEEESE-DEEEACNICFEQACSMEVKECGHQMCAACTLAICCHS 360

Query: 396 KPNPTTACPTAPVCPFCRGSITQLLVAEVKSH 424
           KPNP T     P CPFCR +I++L+VA   S+
Sbjct: 361 KPNPKTLLLHPPACPFCRTTISRLVVATTNSN 380

BLAST of Carg01047 vs. ExPASy Swiss-Prot
Match: Q4FE45 (E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 6.6e-31
Identity = 108/384 (28.12%), Postives = 165/384 (42.97%), Query Frame = 0

Query: 54  ALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNR 113
           A ++GD    + L++ +  +   +T     S LH AAA G  +I+ +LL+   + +  N 
Sbjct: 17  AARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNEIVGLLLENGADVNSRNY 76

Query: 114 HNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAIL---- 173
             QT LM A   G    VQ L+    N+   D    RT LH+AA  GH+ C++ +L    
Sbjct: 77  CGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFAAVNGHARCIRLVLADFL 136

Query: 174 ------SAAHTTPV-----SDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSNGA 233
                 S   T  V     S+    ++FVN     G T LH++A     +CV++LL   A
Sbjct: 137 PSDKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALHMAALNGLFDCVQLLLDLEA 196

Query: 234 LVCVSTCSC--------PGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVAL 293
            V   T            GS+PLH AA GG+L+C + LLA GA +   +  G +P  +A 
Sbjct: 197 NVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARGARKMTLNCNGWLPIDIAR 256

Query: 294 KHKHQACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFV 353
                    LL+P+S           ++I   P +  L                      
Sbjct: 257 MWSRHWLEPLLSPNS----------DVVIPAFPHSNYL---------------------- 316

Query: 354 TPPCPFQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCH 413
               P  S L+I  +     A+   + ++C++C E+ C + A+ C H++C  C L LC  
Sbjct: 317 --SLPLLSILNIAREFGLQSATIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSS 366

BLAST of Carg01047 vs. ExPASy Swiss-Prot
Match: Q7EZ44 (Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS35 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 6.6e-31
Identity = 113/412 (27.43%), Postives = 175/412 (42.48%), Query Frame = 0

Query: 34  GGMGQGLSCADRHGTGV-FFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAAN 93
           G +G    C+    TG     A ++GD++   AL+E++  +   +T     S LH +AA 
Sbjct: 5   GMVGDSFGCS---ATGERLVSAARDGDIQEAMALLELNPRLARYSTFGIRNSPLHYSAAK 64

Query: 94  GQTDILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTC 153
           G  +I+S+L++  V+ ++ N   QT LM A + G    VQ L+   ANI   D     T 
Sbjct: 65  GHHEIVSLLIESGVDINLRNCRGQTALMQACLYGHWKVVQILVLFKANIHKKDCFSGATA 124

Query: 154 LHYAAYYGHSDCLQAILSAAHTTPVSDSW-------------------GFARFVNIRDGD 213
           +H+AA  GH+ CL+ +L A +   + + W                      R +N +   
Sbjct: 125 IHFAALKGHTRCLR-LLVADYVPSLPEFWSVMHAKCTDETNKEAFDAVALRRLINNKSDG 184

Query: 214 GSTPLHLSARQSQPECVRILLSNGALVCVSTCS--------CPGSSPLHLAARGGSLECV 273
           G TPLHL+A     ECV++LL  GA V   T +          GS+PLH AA GGS  C 
Sbjct: 185 GVTPLHLAALHGHAECVQLLLDLGASVSEVTINDGSTIDLIGSGSTPLHYAACGGSAVCC 244

Query: 274 RELLAWGADRFQFDSYGRIPFTVALKHKHQACAALLNPSSPEPLVWPSPLKLIIELHPDA 333
           + L+A GA+    ++ G  P  VA      +   +L   S  P          + + P +
Sbjct: 245 QLLVAAGANMRAQNTNGLTPLMVARSWHKSSVEGILTKRSEVP----------VRILPSS 304

Query: 334 KVLLEKALMDANMEREKAIFKETFVTPPCPFQSDLDIDSDLDNDLASEASDTELCSICFE 393
              L   LM       +  +++T V+  C                       + C+IC +
Sbjct: 305 --YLSLPLMSIVKIARECGWRKTSVSSVC----------------------HDPCAICLD 364

Query: 394 QMCNIEAQPCGHRMCAHCTLSLCCHKKPNPT-TACPTAPVCPFCRGSITQLL 417
             C + A+ CGH  C  C L LC     + +    P +  CP CR +I   +
Sbjct: 365 TECTVSAEGCGHEFCTKCALYLCATASSSTSIRGVPGSIPCPLCRHTIVSFV 378

BLAST of Carg01047 vs. ExPASy TrEMBL
Match: A0A6J1I3N0 (putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita maxima OX=3661 GN=LOC111470249 PE=4 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 435/436 (99.77%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT
Sbjct: 1   MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY
Sbjct: 61  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS
Sbjct: 121 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 180

Query: 216 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 275
           NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 276 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 335
           ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP
Sbjct: 241 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 300

Query: 336 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 395
           FQSDLDIDSDLDNDLASEASDTELCSICFEQ+CNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 FQSDLDIDSDLDNDLASEASDTELCSICFEQICNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 396 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 455
           TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI
Sbjct: 361 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 420

Query: 456 LFGKLSEQNSGTCVKS 472
           LFGKLSEQNSGTCVKS
Sbjct: 421 LFGKLSEQNSGTCVKS 436

BLAST of Carg01047 vs. ExPASy TrEMBL
Match: A0A6J1GBC7 (putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita moschata OX=3662 GN=LOC111452429 PE=4 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 1.9e-254
Identity = 434/436 (99.54%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT
Sbjct: 1   MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY
Sbjct: 61  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS
Sbjct: 121 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 180

Query: 216 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 275
           NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQ+DSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQYDSYGRIPFTVALKHKHQ 240

Query: 276 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 335
           ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP
Sbjct: 241 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 300

Query: 336 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 395
           FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 396 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 455
           TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEEN+SLKSLSI
Sbjct: 361 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENTSLKSLSI 420

Query: 456 LFGKLSEQNSGTCVKS 472
           LFGKLSEQNSGTCVKS
Sbjct: 421 LFGKLSEQNSGTCVKS 436

BLAST of Carg01047 vs. ExPASy TrEMBL
Match: A0A6J1CX01 (putative E3 ubiquitin-protein ligase XBAT31 OS=Momordica charantia OX=3673 GN=LOC111014993 PE=4 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.3e-218
Identity = 374/432 (86.57%), Postives = 400/432 (92.59%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQGLSCADRHG+G FF ALQNG+VEVVRA++E D SILH+TT H+  SALH+AAA+GQ 
Sbjct: 1   MGQGLSCADRHGSG-FFGALQNGEVEVVRAMVETDQSILHRTTNHRRSSALHMAAASGQI 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           DILS+LLD + NPDVLNRHNQTPLMLAAM GKIACV+RLIEAGANILMFDS HRRTCLHY
Sbjct: 61  DILSLLLDRSANPDVLNRHNQTPLMLAAMGGKIACVKRLIEAGANILMFDSIHRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AA+YGHSDCLQAILSAAH+TPVSDSWGFARFVNIRDGDGSTPLHL++RQSQPECVRILL+
Sbjct: 121 AAHYGHSDCLQAILSAAHSTPVSDSWGFARFVNIRDGDGSTPLHLASRQSQPECVRILLN 180

Query: 216 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 275
           NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPF VA+KHKHQ
Sbjct: 181 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFAVAVKHKHQ 240

Query: 276 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 335
           ACAALLNPSSPEPLVWPSPLKLIIEL PDAKVLLEKALMDANMEREK I KET +TPPCP
Sbjct: 241 ACAALLNPSSPEPLVWPSPLKLIIELDPDAKVLLEKALMDANMEREKTILKETCITPPCP 300

Query: 336 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 395
            QSD+DID+D+DNDLA EASD ELC ICFEQ+CNIEAQPC HRMCAHCT+SLCCHKKPNP
Sbjct: 301 LQSDVDIDADVDNDLALEASDAELCCICFEQVCNIEAQPCSHRMCAHCTVSLCCHKKPNP 360

Query: 396 TTACPTAPVCPFCRGSITQLLVAEVKSHDKVDQEISPLKLKGLTTSNFNEENSSLKSLSI 455
           TTACPTAPVCPFCR SITQLLVAEVKS+D  DQEISPLKLKG  TSNFNEENSS KSLS 
Sbjct: 361 TTACPTAPVCPFCRSSITQLLVAEVKSNDNADQEISPLKLKGSRTSNFNEENSSFKSLSA 420

Query: 456 LFGKLSEQNSGT 468
           L GKL E +SG+
Sbjct: 421 LVGKLGEHDSGS 431

BLAST of Carg01047 vs. ExPASy TrEMBL
Match: A0A5A7TRQ8 (Putative E3 ubiquitin-protein ligase XBAT31 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001730 PE=4 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.2e-218
Identity = 370/436 (84.86%), Postives = 401/436 (91.97%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQGLSC  RH TG FFRAL NGDV+V+RA+++ D SILH T  HQ  + LH AAANGQ 
Sbjct: 1   MGQGLSCTHRHETGFFFRALHNGDVDVIRAMVDTDPSILHHTINHQRSTPLHAAAANGQI 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           DILS+LLD NVNPD+LNRHNQTPLMLAAMSG+IACVQRLIEAGANILMFDS HRRTCLHY
Sbjct: 61  DILSILLDRNVNPDILNRHNQTPLMLAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AA+YGHSDCLQAILSAAHTTPVSDSWGFARFVN+RDGDGSTPLHL++RQSQPECVR+LL+
Sbjct: 121 AAFYGHSDCLQAILSAAHTTPVSDSWGFARFVNVRDGDGSTPLHLASRQSQPECVRMLLN 180

Query: 216 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 275
           NGALV VSTCSC GSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVSVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 276 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 335
            CAALLNPSSPEPL+WPSPLKLII+L PDAKVLLEKALMDANMEREKAI KETF+T  CP
Sbjct: 241 TCAALLNPSSPEPLIWPSPLKLIIKLDPDAKVLLEKALMDANMEREKAILKETFITSQCP 300

Query: 336 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 395
            ++D+DID+ +DNDL SEASDT+LC ICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 LEADVDIDAYVDNDLTSEASDTDLCCICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 396 TTACPTAPVCPFCRGSITQLLVAEVK-SHDKVDQEISPLKLKGLTTSNFNEENSSLKSLS 455
           TTACPTAPVCPFCR SI++LLVAEVK ++D VDQEISPLKLKGL TSNFNEEN+SLKSLS
Sbjct: 361 TTACPTAPVCPFCRSSISKLLVAEVKNNNDSVDQEISPLKLKGLRTSNFNEENNSLKSLS 420

Query: 456 ILFGKLSEQNSGTCVK 471
            LFGK  EQN+GTC+K
Sbjct: 421 ALFGKFGEQNAGTCIK 436

BLAST of Carg01047 vs. ExPASy TrEMBL
Match: A0A1S3B5H1 (putative E3 ubiquitin-protein ligase XBAT31 OS=Cucumis melo OX=3656 GN=LOC103486391 PE=4 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.4e-217
Identity = 369/436 (84.63%), Postives = 400/436 (91.74%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQGLSC  RH TG FFRAL NGDV+V+RA+++ D SILH T  HQ  + LH AAANGQ 
Sbjct: 1   MGQGLSCTHRHETGFFFRALHNGDVDVIRAMVDTDPSILHHTINHQRSTPLHAAAANGQI 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           DILS+ LD NVNPD+LNRHNQTPLMLAAMSG+IACVQRLIEAGANILMFDS HRRTCLHY
Sbjct: 61  DILSIPLDRNVNPDILNRHNQTPLMLAAMSGRIACVQRLIEAGANILMFDSIHRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AA+YGHSDCLQAILSAAHTTPVSDSWGFARFVN+RDGDGSTPLHL++RQSQPECVR+LL+
Sbjct: 121 AAFYGHSDCLQAILSAAHTTPVSDSWGFARFVNVRDGDGSTPLHLASRQSQPECVRMLLN 180

Query: 216 NGALVCVSTCSCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 275
           NGALV VSTCSC GSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ
Sbjct: 181 NGALVSVSTCSCAGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKHQ 240

Query: 276 ACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPCP 335
            CAALLNPSSPEPL+WPSPLKLII+L PDAKVLLEKALMDANMEREKAI KETF+T  CP
Sbjct: 241 TCAALLNPSSPEPLIWPSPLKLIIKLDPDAKVLLEKALMDANMEREKAILKETFITSQCP 300

Query: 336 FQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 395
            ++D+DID+ +DNDL SEASDT+LC ICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP
Sbjct: 301 LEADVDIDAYVDNDLTSEASDTDLCCICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPNP 360

Query: 396 TTACPTAPVCPFCRGSITQLLVAEVK-SHDKVDQEISPLKLKGLTTSNFNEENSSLKSLS 455
           TTACPTAPVCPFCR SI++LLVAEVK ++D VDQEISPLKLKGL TSNFNEEN+SLKSLS
Sbjct: 361 TTACPTAPVCPFCRSSISKLLVAEVKNNNDSVDQEISPLKLKGLRTSNFNEENNSLKSLS 420

Query: 456 ILFGKLSEQNSGTCVK 471
            LFGK  EQN+GTC+K
Sbjct: 421 ALFGKFGEQNAGTCIK 436

BLAST of Carg01047 vs. TAIR 10
Match: AT2G28840.1 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 503.8 bits (1296), Expect = 1.5e-142
Identity = 262/450 (58.22%), Postives = 328/450 (72.89%), Query Frame = 0

Query: 36  MGQGLSCADRHGTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQT 95
           MGQ +SC  R   G+ F ++Q GD+  +R +M  + S+L+QTT +   S LHVAAANGQ 
Sbjct: 1   MGQSMSCGSRPEHGI-FASVQCGDIITIRRVMATEPSLLNQTTPYDRHSVLHVAAANGQI 60

Query: 96  DILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHY 155
           +ILS+LL+   NPD+LNRH QTPLMLAAM G+I+CV++L E GANILMFDS +RRTCLHY
Sbjct: 61  EILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHY 120

Query: 156 AAYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLS 215
           AAYYGH++C+QAILSAA ++PV+  WG+ARFVNIRD  G+TPLHL+ARQ +PECV +LL 
Sbjct: 121 AAYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLLD 180

Query: 216 NGALVCVSTC--SCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHK 275
           +G+LVC ST     PGS+PLHLAAR GS++CVR+LLAWGADR Q D+ GRIP+ VA+KHK
Sbjct: 181 SGSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKHK 240

Query: 276 HQACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPP 335
           H AC ALLNPSS EPLVWPSPLK I EL+ +AK+LLE+ALM+AN EREK I K T  + P
Sbjct: 241 HGACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSLP 300

Query: 336 CPFQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKP 395
            P  SD D       D  SE SDTELC ICFEQ+C IE + CGH+MCA CTL+LCCH KP
Sbjct: 301 SPSFSDTD-------DNMSEVSDTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNKP 360

Query: 396 NPTTACPTAPVCPFCRGSITQLLVAEVKSHDK-----------VDQE---ISPLKL-KGL 455
           NPTT+  T PVCPFCR +I  L+VA+  +++            VD+E   +S  K  K  
Sbjct: 361 NPTTSTVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKHR 420

Query: 456 TTSNFNEENSSLKSLSIL--FGKLSEQNSG 467
            + N  EE+SS   LS +  FG+++ + SG
Sbjct: 421 RSINLGEESSSFMGLSTIGSFGRITGRGSG 442

BLAST of Carg01047 vs. TAIR 10
Match: AT2G28840.2 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 453.0 bits (1164), Expect = 3.0e-127
Identity = 234/389 (60.15%), Postives = 288/389 (74.04%), Query Frame = 0

Query: 97  ILSMLLDWNVNPDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYA 156
           ILS+LL+   NPD+LNRH QTPLMLAAM G+I+CV++L E GANILMFDS +RRTCLHYA
Sbjct: 47  ILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHYA 106

Query: 157 AYYGHSDCLQAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSN 216
           AYYGH++C+QAILSAA ++PV+  WG+ARFVNIRD  G+TPLHL+ARQ +PECV +LL +
Sbjct: 107 AYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLLDS 166

Query: 217 GALVCVSTC--SCPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVALKHKH 276
           G+LVC ST     PGS+PLHLAAR GS++CVR+LLAWGADR Q D+ GRIP+ VA+KHKH
Sbjct: 167 GSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKHKH 226

Query: 277 QACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFVTPPC 336
            AC ALLNPSS EPLVWPSPLK I EL+ +AK+LLE+ALM+AN EREK I K T  + P 
Sbjct: 227 GACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSLPS 286

Query: 337 PFQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCHKKPN 396
           P  SD D       D  SE SDTELC ICFEQ+C IE + CGH+MCA CTL+LCCH KPN
Sbjct: 287 PSFSDTD-------DNMSEVSDTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNKPN 346

Query: 397 PTTACPTAPVCPFCRGSITQLLVAEVKSHDK-----------VDQE---ISPLKL-KGLT 456
           PTT+  T PVCPFCR +I  L+VA+  +++            VD+E   +S  K  K   
Sbjct: 347 PTTSTVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKHRR 406

Query: 457 TSNFNEENSSLKSLSIL--FGKLSEQNSG 467
           + N  EE+SS   LS +  FG+++ + SG
Sbjct: 407 SINLGEESSSFMGLSTIGSFGRITGRGSG 428

BLAST of Carg01047 vs. TAIR 10
Match: AT5G07270.1 (XB3 ortholog 3 in Arabidopsis thaliana )

HSP 1 Score: 136.7 bits (343), Expect = 4.7e-32
Identity = 108/384 (28.12%), Postives = 165/384 (42.97%), Query Frame = 0

Query: 54  ALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNR 113
           A ++GD    + L++ +  +   +T     S LH AAA G  +I+ +LL+   + +  N 
Sbjct: 17  AARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNEIVGLLLENGADVNSRNY 76

Query: 114 HNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAIL---- 173
             QT LM A   G    VQ L+    N+   D    RT LH+AA  GH+ C++ +L    
Sbjct: 77  CGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFAAVNGHARCIRLVLADFL 136

Query: 174 ------SAAHTTPV-----SDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILLSNGA 233
                 S   T  V     S+    ++FVN     G T LH++A     +CV++LL   A
Sbjct: 137 PSDKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALHMAALNGLFDCVQLLLDLEA 196

Query: 234 LVCVSTCSC--------PGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVAL 293
            V   T            GS+PLH AA GG+L+C + LLA GA +   +  G +P  +A 
Sbjct: 197 NVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARGARKMTLNCNGWLPIDIAR 256

Query: 294 KHKHQACAALLNPSSPEPLVWPSPLKLIIELHPDAKVLLEKALMDANMEREKAIFKETFV 353
                    LL+P+S           ++I   P +  L                      
Sbjct: 257 MWSRHWLEPLLSPNS----------DVVIPAFPHSNYL---------------------- 316

Query: 354 TPPCPFQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTLSLCCH 413
               P  S L+I  +     A+   + ++C++C E+ C + A+ C H++C  C L LC  
Sbjct: 317 --SLPLLSILNIAREFGLQSATIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSS 366

BLAST of Carg01047 vs. TAIR 10
Match: AT5G57740.1 (XB3 ortholog 2 in Arabidopsis thaliana )

HSP 1 Score: 124.0 bits (310), Expect = 3.2e-28
Identity = 104/389 (26.74%), Postives = 162/389 (41.65%), Query Frame = 0

Query: 54  ALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNVNPDVLNR 113
           A ++GD++  +AL++ +  +   +T     S LH +AA G  +I+S+L++  V+ ++ N 
Sbjct: 23  AARDGDLQEAKALLDYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLVESGVDINLRNY 82

Query: 114 HNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCLQAILSAAH 173
             QT LM A   G    V  LI  GANI   D  +  T LH AA  GH  C++ +LS  +
Sbjct: 83  RGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGTALHLAALNGHPRCIRILLS-EY 142

Query: 174 TTPVSDSWGFAR----------------FVNIRDGDGSTPLHLSARQSQPECVRILLSNG 233
              V + W   +                 +N     G TPLH++A     E V++LL  G
Sbjct: 143 IPSVPNCWSLLKNKKTSVAGFDSSVLHEVINRAADGGITPLHVAALNGHIETVQLLLDLG 202

Query: 234 ALVCVSTCS--------CPGSSPLHLAARGGSLECVRELLAWGADRFQFDSYGRIPFTVA 293
           A V   T            GS+ LH A+ GG+ +C + L++ GA     +S G  P  VA
Sbjct: 203 ASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCCQLLISKGACLAAVNSNGWTPMMVA 262

Query: 294 LKHKHQACAALLNPSSPEPLVW----PSPLKLIIELHPDAKVLLEKALMDANMEREKAIF 353
                     +LNP++ +P +     PSP   +              LM      ++  +
Sbjct: 263 RSWHRNWLEEILNPTTEQPQLHLPNVPSPFLCL-------------PLMSIVNIAQECGW 322

Query: 354 KETFVTPPCPFQSDLDIDSDLDNDLASEASDTELCSICFEQMCNIEAQPCGHRMCAHCTL 413
           +E     PC                       + C++C E+ C + A  C H  C +C L
Sbjct: 323 RENDCLTPC----------------------RDPCAVCLERKCTVAADGCAHEFCTNCAL 375

BLAST of Carg01047 vs. TAIR 10
Match: AT3G12360.1 (Ankyrin repeat family protein )

HSP 1 Score: 82.8 bits (203), Expect = 8.1e-16
Identity = 62/206 (30.10%), Postives = 95/206 (46.12%), Query Frame = 0

Query: 47  GTGVFFRALQNGDVEVVRALMEIDSSILHQTTKHQGLSALHVAAANGQTDILSMLLDWNV 106
           G    F A   G ++VV+ L++  S          G   LH+AA  G   I+ +LLD + 
Sbjct: 129 GETALFTAADKGHLDVVKELLKYSSRESIAKKNRSGYDPLHIAAIQGHHAIVEVLLDHDA 188

Query: 107 N-PDVLNRHNQTPLMLAAMSGKIACVQRLIEAGANILMFDSTHRRTCLHYAAYYGHSDCL 166
                    N TPL+ AAM G    V +L+    N+L    ++ +  LH AA  GH + +
Sbjct: 189 TLSQTFGPSNATPLVSAAMRGHTEVVNQLLSKAGNLLEISRSNNKNALHLAARQGHVEVI 248

Query: 167 QAILSAAHTTPVSDSWGFARFVNIRDGDGSTPLHLSARQSQPECVRILL-SNGALVCVST 226
           +A+LS             AR +   D  G T LH++ +    E V++LL ++ A+V    
Sbjct: 249 KALLSKDPQ--------LARRI---DKKGQTALHMAVKGQSSEVVKLLLDADPAIVMQPD 308

Query: 227 CSCPGSSPLHLAARGGSLECVRELLA 251
            SC  ++ LH+A R    E V  LL+
Sbjct: 309 KSC--NTALHVATRKKRAEIVELLLS 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027954.15.8e-274100.00putative E3 ubiquitin-protein ligase XBAT31, partial [Cucurbita argyrosperma sub... [more]
KAG6596410.11.9e-26487.69putative E3 ubiquitin-protein ligase XBAT31, partial [Cucurbita argyrosperma sub... [more]
XP_023540882.17.8e-255100.00putative E3 ubiquitin-protein ligase XBAT31 [Cucurbita pepo subsp. pepo][more]
XP_022971571.12.3e-25499.77putative E3 ubiquitin-protein ligase XBAT31 [Cucurbita maxima][more]
XP_022948919.13.9e-25499.54putative E3 ubiquitin-protein ligase XBAT31 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q94B552.1e-14158.22Putative E3 ubiquitin-protein ligase XBAT31 OS=Arabidopsis thaliana OX=3702 GN=X... [more]
Q65XV21.0e-14058.03E3 ubiquitin-protein ligase XB3 OS=Oryza sativa subsp. japonica OX=39947 GN=XB3 ... [more]
Q94CT72.2e-11454.59Probable E3 ubiquitin-protein ligase XBOS31 OS=Oryza sativa subsp. japonica OX=3... [more]
Q4FE456.6e-3128.13E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=... [more]
Q7EZ446.6e-3127.43Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1I3N01.1e-25499.77putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GBC71.9e-25499.54putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1CX011.3e-21886.57putative E3 ubiquitin-protein ligase XBAT31 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A5A7TRQ82.2e-21884.86Putative E3 ubiquitin-protein ligase XBAT31 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B5H11.4e-21784.63putative E3 ubiquitin-protein ligase XBAT31 OS=Cucumis melo OX=3656 GN=LOC103486... [more]
Match NameE-valueIdentityDescription
AT2G28840.11.5e-14258.22XB3 ortholog 1 in Arabidopsis thaliana [more]
AT2G28840.23.0e-12760.15XB3 ortholog 1 in Arabidopsis thaliana [more]
AT5G07270.14.7e-3228.13XB3 ortholog 3 in Arabidopsis thaliana [more]
AT5G57740.13.2e-2826.74XB3 ortholog 2 in Arabidopsis thaliana [more]
AT3G12360.18.1e-1630.10Ankyrin repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 228..257
e-value: 0.14
score: 21.3
coord: 46..75
e-value: 420.0
score: 7.8
coord: 261..290
e-value: 890.0
score: 5.4
coord: 81..110
e-value: 0.026
score: 23.7
coord: 114..143
e-value: 2.3E-4
score: 30.5
coord: 193..222
e-value: 0.001
score: 28.4
coord: 148..177
e-value: 0.025
score: 23.7
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 81..113
score: 11.70115
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 193..218
score: 11.48747
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 114..146
score: 11.62102
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 228..260
score: 11.94154
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 181..283
e-value: 7.2E-23
score: 83.0
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 36..180
e-value: 1.2E-31
score: 112.1
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 43..282
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 338..420
e-value: 1.4E-7
score: 32.8
NoneNo IPR availablePFAMPF13857Ank_5coord: 229..269
e-value: 2.4E-7
score: 30.9
NoneNo IPR availablePANTHERPTHR24128:SF14E3 UBIQUITIN-PROTEIN LIGASE XBAT31-RELATEDcoord: 36..468
NoneNo IPR availablePANTHERPTHR24128HOMEOBOX PROTEIN WARIAIcoord: 36..468
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 114..141
score: 10.471169
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 228..254
score: 11.426298
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 81..113
score: 10.338512
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 193..218
score: 11.452829
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 355..412
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 149..219
e-value: 7.6E-10
score: 39.3
coord: 52..145
e-value: 1.1E-14
score: 54.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 360..409
score: 10.055033

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01047-RACarg01047-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
biological_process GO:0016310 phosphorylation
biological_process GO:0009408 response to heat
molecular_function GO:0140096 catalytic activity, acting on a protein
molecular_function GO:0016301 kinase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0005515 protein binding