Carg01002 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01002
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Low temperature and salt responsive protein family
Location	Carg_Chr06: 1704898 .. 1705346 (-)
RNA-Seq Expression	Carg01002
Synteny	Carg01002

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAGGTTTTAAAAGGGGGGCGATGAAGAGGTCTGGGCTCGCGGCGGCTATTAGCTAAGGGCTACGTGAAAATGCAAGGCGAACGAATCCCAAAGCGAATGGGCTCAGCCACCTTCGTAGAAGTTCTTATCGCTATCTTCATCCCTCCCCTCGGCGTCTTCCTCCGCTATGGCATTGGGGTAATCTCCTCTCCCTCTCTCCTTTCGATTCGTTCCAGAACTTGATCCGAATTCAAACTTCTGATATCTTGATCGATCCATCTGATCTATGTTCGTTCGAACAACATTTTAATTAATTAATTAATTAATTTTGTTGTATAAAATTTGCAGGTAGAATTTTGGATTGATTTGGTGCTCACATTTTTGGGGTACCTTCCTGGAATTATTTACGCCGTTTATGTTTTGGTGGGGATGTGATGAAAAAAATATTGTTTTTTTATTTTTTTATTT

mRNA sequence

AAGAGGTTTTAAAAGGGGGGCGATGAAGAGGTCTGGGCTCGCGGCGGCTATTAGCTAAGGGCTACGTGAAAATGCAAGGCGAACGAATCCCAAAGCGAATGGGCTCAGCCACCTTCGTAGAAGTTCTTATCGCTATCTTCATCCCTCCCCTCGGCGTCTTCCTCCGCTATGGCATTGGGGTAGAATTTTGGATTGATTTGGTGCTCACATTTTTGGGGTACCTTCCTGGAATTATTTACGCCGTTTATGTTTTGGTGGGGATGTGATGAAAAAAATATTGTTTTTTTATTTTTTTATTT

Coding sequence (CDS)

ATGCAAGGCGAACGAATCCCAAAGCGAATGGGCTCAGCCACCTTCGTAGAAGTTCTTATCGCTATCTTCATCCCTCCCCTCGGCGTCTTCCTCCGCTATGGCATTGGGGTAGAATTTTGGATTGATTTGGTGCTCACATTTTTGGGGTACCTTCCTGGAATTATTTACGCCGTTTATGTTTTGGTGGGGATGTGA

Protein sequence

MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVLVGM

Homology

[Close]

BLAST of Carg01002 vs. NCBI nr
Match: XP_022945600.1 (low temperature-induced protein lt101.2-like [Cucurbita moschata] >XP_023005767.1 low temperature-induced protein lt101.2-like [Cucurbita maxima] >XP_023540674.1 low temperature-induced protein lt101.2-like [Cucurbita pepo subsp. pepo] >KAG7027999.1 Low temperature-induced protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 126.3 bits (316), Expect = 9.3e-26
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV 60
          MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV
Sbjct: 1  MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV 60

Query: 61 LVGM 65
          LVGM
Sbjct: 61 LVGM 64

[Close]

BLAST of Carg01002 vs. NCBI nr
Match: XP_038906189.1 (low temperature-induced protein lt101.2-like [Benincasa hispida])

HSP 1 Score: 118.2 bits (295), Expect = 2.5e-23
Identity = 57/63 (90.48%), Postives = 61/63 (96.83%), Query Frame = 0


Query: 1  MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV 60
          MQG+RIPKRMGSATF+EVL+AIFIPPLGVFLRYGIG+EFWIDLVLT  GYLPGIIYAVYV
Sbjct: 16 MQGDRIPKRMGSATFIEVLLAIFIPPLGVFLRYGIGIEFWIDLVLTLFGYLPGIIYAVYV 75

Query: 61 LVG 64
          LVG
Sbjct: 76 LVG 78

[Close]

BLAST of Carg01002 vs. NCBI nr
Match: XP_022145681.1 (low temperature-induced protein lt101.2-like [Momordica charantia])

HSP 1 Score: 117.9 bits (294), Expect = 3.3e-23
Identity = 56/64 (87.50%), Postives = 63/64 (98.44%), Query Frame = 0


Query: 1  MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV 60
          MQG+RIPKRMGSATF+EVL+A+FIPPLGVFLRYGIG+EFWI L+LTFLGYLPGI+YAVYV
Sbjct: 1  MQGDRIPKRMGSATFLEVLLAVFIPPLGVFLRYGIGIEFWIALLLTFLGYLPGIVYAVYV 60

Query: 61 LVGM 65
          LVGM
Sbjct: 61 LVGM 64

[Close]

BLAST of Carg01002 vs. NCBI nr
Match: XP_004137807.1 (low temperature-induced protein lt101.2 [Cucumis sativus] >KGN58938.1 hypothetical protein Csa_001360 [Cucumis sativus])

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-21
Identity = 53/62 (85.48%), Postives = 60/62 (96.77%), Query Frame = 0


Query: 2  QGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVL 61
          Q +RIPKRMGSATF+EVL+AIFIPP+GVFLRYGIG+EFWIDLVLTF GY+PGIIYAVYVL
Sbjct: 6  QVDRIPKRMGSATFIEVLLAIFIPPVGVFLRYGIGIEFWIDLVLTFFGYIPGIIYAVYVL 65

Query: 62 VG 64
          +G
Sbjct: 66 IG 67

[Close]

BLAST of Carg01002 vs. NCBI nr
Match: XP_008442650.1 (PREDICTED: low temperature-induced protein lt101.2-like [Cucumis melo])

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-21
Identity = 53/62 (85.48%), Postives = 60/62 (96.77%), Query Frame = 0


Query: 2  QGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVL 61
          Q +RIPKRMGSATFVEVL+AIFIPP+GVFLRYGIG+EFWIDLVLTF GY+PGI+YAVYVL
Sbjct: 6  QVDRIPKRMGSATFVEVLLAIFIPPVGVFLRYGIGIEFWIDLVLTFFGYIPGIVYAVYVL 65

Query: 62 VG 64
          +G
Sbjct: 66 IG 67

[Close]

BLAST of Carg01002 vs. ExPASy Swiss-Prot
Match: Q9ARD5 (Low temperature-induced protein lt101.2 OS=Hordeum vulgare OX=4513 GN=LT101.2 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 6.9e-16
Identity = 40/53 (75.47%), Postives = 48/53 (90.57%), Query Frame = 0


Query: 10 MGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVLV 63
          M SATF+EV++AI +PP+GVFLRYG+ VEFWI L+LT LGY+PGIIYAVYVLV
Sbjct: 1  MASATFIEVILAIILPPVGVFLRYGLAVEFWICLLLTLLGYIPGIIYAVYVLV 53

[Close]

BLAST of Carg01002 vs. ExPASy Swiss-Prot
Match: Q9ZNQ7 (Hydrophobic protein RCI2A OS=Arabidopsis thaliana OX=3702 GN=RCI2A PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.5e-15
Identity = 39/52 (75.00%), Postives = 47/52 (90.38%), Query Frame = 0


Query: 10 MGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVL 62
          M +ATFV+++IAI +PPLGVFLR+G GVEFWI LVLT LGY+PGIIYA+YVL
Sbjct: 1  MSTATFVDIIIAILLPPLGVFLRFGCGVEFWICLVLTLLGYIPGIIYAIYVL 52

[Close]

BLAST of Carg01002 vs. ExPASy Swiss-Prot
Match: P68178 (Salt stress-induced hydrophobic peptide ESI3 OS=Thinopyrum elongatum OX=4588 GN=ESI3 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.6e-15
Identity = 40/53 (75.47%), Postives = 48/53 (90.57%), Query Frame = 0


Query: 10 MGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVLV 63
          MGSAT +EV++AI +PP+GVFLRY +GVEFWI L+LT LGY+PGIIYAVYVLV
Sbjct: 1  MGSATVLEVILAIILPPVGVFLRYKLGVEFWICLLLTILGYIPGIIYAVYVLV 53

[Close]

BLAST of Carg01002 vs. ExPASy Swiss-Prot
Match: P68179 (Low temperature-induced protein lt101.1 OS=Hordeum vulgare OX=4513 GN=LT101.1 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.6e-15
Identity = 40/53 (75.47%), Postives = 48/53 (90.57%), Query Frame = 0


Query: 10 MGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVLV 63
          MGSAT +EV++AI +PP+GVFLRY +GVEFWI L+LT LGY+PGIIYAVYVLV
Sbjct: 1  MGSATVLEVILAIILPPVGVFLRYKLGVEFWICLLLTILGYIPGIIYAVYVLV 53

[Close]

BLAST of Carg01002 vs. ExPASy Swiss-Prot
Match: Q9ZNS6 (Hydrophobic protein RCI2B OS=Arabidopsis thaliana OX=3702 GN=RCI2B PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.2e-13
Identity = 33/52 (63.46%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 10 MGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVL 62
          M +ATFVE+++AI +PPLGVFL++G  VEFWI L+LT  GYLPGI+YA+Y++
Sbjct: 1  MSTATFVEIILAIILPPLGVFLKFGCKVEFWICLILTLFGYLPGILYALYII 52

[Close]

BLAST of Carg01002 vs. ExPASy TrEMBL
Match: A0A6J1KU26 (low temperature-induced protein lt101.2-like OS=Cucurbita maxima OX=3661 GN=LOC111498668 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.5e-26
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV 60
          MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV
Sbjct: 1  MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV 60

Query: 61 LVGM 65
          LVGM
Sbjct: 61 LVGM 64

[Close]

BLAST of Carg01002 vs. ExPASy TrEMBL
Match: A0A6J1G1E1 (low temperature-induced protein lt101.2-like OS=Cucurbita moschata OX=3662 GN=LOC111449786 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.5e-26
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV 60
          MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV
Sbjct: 1  MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV 60

Query: 61 LVGM 65
          LVGM
Sbjct: 61 LVGM 64

[Close]

BLAST of Carg01002 vs. ExPASy TrEMBL
Match: A0A6J1CX15 (low temperature-induced protein lt101.2-like OS=Momordica charantia OX=3673 GN=LOC111015073 PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.6e-23
Identity = 56/64 (87.50%), Postives = 63/64 (98.44%), Query Frame = 0


Query: 1  MQGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYV 60
          MQG+RIPKRMGSATF+EVL+A+FIPPLGVFLRYGIG+EFWI L+LTFLGYLPGI+YAVYV
Sbjct: 1  MQGDRIPKRMGSATFLEVLLAVFIPPLGVFLRYGIGIEFWIALLLTFLGYLPGIVYAVYV 60

Query: 61 LVGM 65
          LVGM
Sbjct: 61 LVGM 64

[Close]

BLAST of Carg01002 vs. ExPASy TrEMBL
Match: A0A0A0LAU7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G736860 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.1e-22
Identity = 53/62 (85.48%), Postives = 60/62 (96.77%), Query Frame = 0


Query: 2  QGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVL 61
          Q +RIPKRMGSATF+EVL+AIFIPP+GVFLRYGIG+EFWIDLVLTF GY+PGIIYAVYVL
Sbjct: 6  QVDRIPKRMGSATFIEVLLAIFIPPVGVFLRYGIGIEFWIDLVLTFFGYIPGIIYAVYVL 65

Query: 62 VG 64
          +G
Sbjct: 66 IG 67

[Close]

BLAST of Carg01002 vs. ExPASy TrEMBL
Match: A0A1S3B667 (low temperature-induced protein lt101.2-like OS=Cucumis melo OX=3656 GN=LOC103486456 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.1e-22
Identity = 53/62 (85.48%), Postives = 60/62 (96.77%), Query Frame = 0


Query: 2  QGERIPKRMGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVL 61
          Q +RIPKRMGSATFVEVL+AIFIPP+GVFLRYGIG+EFWIDLVLTF GY+PGI+YAVYVL
Sbjct: 6  QVDRIPKRMGSATFVEVLLAIFIPPVGVFLRYGIGIEFWIDLVLTFFGYIPGIVYAVYVL 65

Query: 62 VG 64
          +G
Sbjct: 66 IG 67

[Close]

BLAST of Carg01002 vs. TAIR 10
Match: AT2G38905.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 86.3 bits (212), Expect = 9.9e-18
Identity = 40/54 (74.07%), Postives = 49/54 (90.74%), Query Frame = 0


Query: 10 MGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVLVG 64
          MGS TF+E+++AI +PP+GVFLRYG GVEFWI L+LT LGY+PGIIYA+YVLVG
Sbjct: 1  MGSETFLEIILAILLPPVGVFLRYGCGVEFWICLLLTILGYIPGIIYAIYVLVG 54

[Close]

BLAST of Carg01002 vs. TAIR 10
Match: AT3G05880.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 82.8 bits (203), Expect = 1.1e-16
Identity = 39/52 (75.00%), Postives = 47/52 (90.38%), Query Frame = 0


Query: 10 MGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVL 62
          M +ATFV+++IAI +PPLGVFLR+G GVEFWI LVLT LGY+PGIIYA+YVL
Sbjct: 1  MSTATFVDIIIAILLPPLGVFLRFGCGVEFWICLVLTLLGYIPGIIYAIYVL 52

[Close]

BLAST of Carg01002 vs. TAIR 10
Match: AT3G05890.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 75.1 bits (183), Expect = 2.3e-14
Identity = 33/52 (63.46%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 10 MGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVL 62
          M +ATFVE+++AI +PPLGVFL++G  VEFWI L+LT  GYLPGI+YA+Y++
Sbjct: 1  MSTATFVEIILAIILPPLGVFLKFGCKVEFWICLILTLFGYLPGILYALYII 52

[Close]

BLAST of Carg01002 vs. TAIR 10
Match: AT1G57550.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 72.4 bits (176), Expect = 1.5e-13
Identity = 35/52 (67.31%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 10 MGSATFVEVLIAIFIPPLGVFLRYGIGVEFWIDLVLTFLGYLPGIIYAVYVL 62
          MGS  F+EVL AIFIPP+GVFLRYG+G+EFW+ L+LT   ++PG+IYA+YVL
Sbjct: 1  MGS--FLEVLCAIFIPPVGVFLRYGLGLEFWVCLLLTLFAFIPGLIYAIYVL 50

[Close]

BLAST of Carg01002 vs. TAIR 10
Match: AT4G30650.1 (Low temperature and salt responsive protein family )

HSP 1 Score: 57.0 bits (136), Expect = 6.5e-09
Identity = 33/49 (67.35%), Postives = 39/49 (79.59%), Query Frame = 0


Query: 15 FVEVLIAIFIPPLGVFLRYG-IGVEFWIDLVLTFLGYLPGIIYAVYVLV 63
          F E+LIAI +PPLGV L+ G   VEF I LVLT LGY+PGIIYA+YV+V
Sbjct: 8  FCEILIAILLPPLGVCLKRGCCTVEFLICLVLTILGYIPGIIYALYVIV 56

The following BLAST results are available for this feature:

BLAST of Carg01002 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022945600.1	9.3e-26	100.00	low temperature-induced protein lt101.2-like [Cucurbita moschata] >XP_023005767....	[more]
XP_038906189.1	2.5e-23	90.48	low temperature-induced protein lt101.2-like [Benincasa hispida]	[more]
XP_022145681.1	3.3e-23	87.50	low temperature-induced protein lt101.2-like [Momordica charantia]	[more]
XP_004137807.1	1.1e-21	85.48	low temperature-induced protein lt101.2 [Cucumis sativus] >KGN58938.1 hypothetic...	[more]
XP_008442650.1	1.1e-21	85.48	PREDICTED: low temperature-induced protein lt101.2-like [Cucumis melo]	[more]

BLAST of Carg01002 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ARD5	6.9e-16	75.47	Low temperature-induced protein lt101.2 OS=Hordeum vulgare OX=4513 GN=LT101.2 PE...	[more]
Q9ZNQ7	1.5e-15	75.00	Hydrophobic protein RCI2A OS=Arabidopsis thaliana OX=3702 GN=RCI2A PE=2 SV=1	[more]
P68178	2.6e-15	75.47	Salt stress-induced hydrophobic peptide ESI3 OS=Thinopyrum elongatum OX=4588 GN=...	[more]
P68179	2.6e-15	75.47	Low temperature-induced protein lt101.1 OS=Hordeum vulgare OX=4513 GN=LT101.1 PE...	[more]
Q9ZNS6	3.2e-13	63.46	Hydrophobic protein RCI2B OS=Arabidopsis thaliana OX=3702 GN=RCI2B PE=2 SV=1	[more]

BLAST of Carg01002 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KU26	4.5e-26	100.00	low temperature-induced protein lt101.2-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1G1E1	4.5e-26	100.00	low temperature-induced protein lt101.2-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1CX15	1.6e-23	87.50	low temperature-induced protein lt101.2-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A0A0LAU7	5.1e-22	85.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G736860 PE=3 SV=1	[more]
A0A1S3B667	5.1e-22	85.48	low temperature-induced protein lt101.2-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]

BLAST of Carg01002 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38905.1	9.9e-18	74.07	Low temperature and salt responsive protein family	[more]
AT3G05880.1	1.1e-16	75.00	Low temperature and salt responsive protein family	[more]
AT3G05890.1	2.3e-14	63.46	Low temperature and salt responsive protein family	[more]
AT1G57550.1	1.5e-13	67.31	Low temperature and salt responsive protein family	[more]
AT4G30650.1	6.5e-09	67.35	Low temperature and salt responsive protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000612	Proteolipid membrane potential modulator	PFAM	PF01679	Pmp3	coord: 15..62 e-value: 5.5E-19 score: 68.1
IPR000612	Proteolipid membrane potential modulator	PANTHER	PTHR21659	HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED	coord: 10..62
IPR000612	Proteolipid membrane potential modulator	PROSITE	PS01309	UPF0057	coord: 19..34
None	No IPR available	PANTHER	PTHR21659:SF65	OS03G0286900 PROTEIN	coord: 10..62

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01002-RA	Carg01002-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane