Carg00973 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00973
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionOTU domain-containing protein
LocationCarg_Chr06: 1824482 .. 1829730 (+)
RNA-Seq ExpressionCarg00973
SyntenyCarg00973
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGATTTTTCAATCAAAAGCCGCAAAGGGTCGGCCACAACCAACCCAACAAATCTCAGTCGCTTGATTTTCCACCATCCATTCGAAATCCAATCTCATCCGTTCTTCGCCGCCTCCGTTCTTTGACCATCGCCGATCTCCACCGTCAAATCCGACCTCTGAAATTAACACCCATGATTTCTTGATTACGGGGTTTATCATTCTTTTTTTCTTAAACTAGAATCCCATCACATCAATCGTTGAAGTATTAGCATGAAACTTGGCAGCCATAGCCAGTTCTTTTGAACAAACTCGCTCGAAAGGGCGTTTAAACGGCTCGAAAGGGCGACTATACTGTATAGATAGATGTATATGGATTCATATGGCATGAGTTTCGACTGTTTGTTTTAATTTATTGATTTCTGTTCTTTTGCCCATTTTCATTTTCAATGCGTTCATCGTCATCCTCGGTGCTGATTAACTTTCCGTAATTGCTCCCTGACCTGTGAACCTCTTATTTCCTGTTCTACCCACCTTGTTCATTCGAGCTAAAACGGAGTTTTCGTTCGTGGCTGCGAATTCAAGGTAATTTGTGACTGGAAATTGGACGTATATGCTTCGATTATGTCGAACTTGTTCTTGTTTATTGAATCGCCAGGAAAGGATTACACATATGCATTCATTTCTTGAAAACATCTTGAAATTCTTACTTGTTTTTGTATCTAGATGGTTCCTTCTGGTTATGAAGCGGATGTTATTGGGTGGGGTCGTCGTCTTTTTGATGATGATTCAGTTTTTAATTCTGGGTATTACGGTGAGATGACAACAGTAGATGACCATTACCCTGGGAATTATTACAGAGATCATTACAATTTAGAACAGAATTCCGTGGAGAATGATGAGATTATTGCTAGAACTCTGCAAGAAAACTTGTCACAGTTATCAATTACAGACTCCTCTAGATACCCAGTTGAAAGAGAGGAGCCACTGCAAGGATCCATGTATACAATTGGTTGGCATAATTCATTTCCTAGGAATAACAATTCAGGTATTCATTATGCCTTTTTGTTCCAATGTTCATCTCTGAATTGTATAGCTTATATGTTTGTTCTTGATGACATTCTAAGTTCTTATTGTAGAGAGTATTTCTTCTCCTGAGGAAGATTTTGAAACTCATGATCCTCCCAGTTCATGTTCTAGTCTTGGGGATGAGAACTTCTATTCACATGCTGTTGATGGTGAAGAACTCTGGAGATTCAATCAAATGATTCCTGTTCCTGTAGGTTTAAATACATAAAATTCTGTCTCTTTGCATCATGTTCATTTCTTGTGTAGACTCTAATTTGTTTGGCGCTTCGCCTCTTTACATGTTCTGTACTTGCAGAGTCTTGGAGATGGTTGTTTTTGCCTCAACCTCCTTATGTTTTGTGTCATAGTCTTATTTCTTTTCTTACTTATCTATGCAGCTCTAGTGCTCTTTATCCATGTAATTTCTACCTGTTGATGATTGTTGGGAGAGGAGTCCCGTATCGGCTAATTAAGGGGTTGATCATGGGTTTGTAAGTAAAGAATATTATCTCTATTGGTACGAGGCCTTTTGGGGAAACCAAAATCGAAGCCATGAGAGCTTATACTCAAAGTGGACAATATCATACCATTGTGGAGATTCGTGGTTCCTAACATGGTATCAGAGTCATGCCCTTTATTTAGTCATGTCAATAGAATCCTCAAATGTCAAACAATGAAGTTGTGAGCCTCGAAGGTGTAGTCAAAAGTAACTCAAGTGCAAACAAAGGGTGTACTTTGTTCGAGAGCTCTAGAGAAGGAGTCGAGCCTCGATTAAGGGGGGAGGCTGTTCGAGGGCTCCATAGGCCTCAAGGTAGGCTCTATGGTGTACTTTGTTCGAGTGGAGGATTGTTGGGAGAGGAGTCCCACATCGGCTAATTAAGGGGTTGATCATGGGTTTATAAGTAAGGAATACTATTGCGGTTGGTACGAGGTCTTTTGGGGAAACCAAAAGCAAAACCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTAGAGATTCGTGGTTTATAACATCCACTTCATCTATACTGCTTGTTGCTGTAGTTTTTTGTGGATTATAAGACGTCTGTTTATTTTCGATTTTCTTCTGAGCGTACAGTTTTGGGACGAGACTAACCATTTCTGTGAATTGAGCAGCATGTTCCTAGAATTAACGGAGAGATTCCTTCAGTTGATGAAGCTGCTTCTGATCATGAAAGGCTGCTGAACAGGTATCTTCTCAAAATTTTGTACGTCTGAGAAATTGGTCTTTAAACTGTACAATAAGAGGAACCTACGATGCCATAGCCTTCTTAATATATAGTCATATTCTCTAGCTACCTTAAATCTTCTGAGAAATTGTATAATAACTTAGGAAATTTCTTCTCAATTCAGATTACAAGTGTATGACTTTGTTGAACGAAAGGTTCAAGGTGATGGCAATTGTCAGGTTTGTTATTCCCTGCTTTCTATCTTTTTACTTTTCTTTTGTTGGTTTTTTCTGCTGATTTTTGGTTCATTTTAAATAATGGTAATCGGTTCGGTCACGGTCATATTCATTCGTTTGCTTTCTGTATTCTTTCCCAGTTTCGTGCATTATCTGATCAATTATATGGAACACCTGACAATCACGAACTCGTGAGACAGAAAGTTGTAGATCAGGTTTGTCATCCTGGAAAACCGAAACACGATCATTGATATCACGAATTGGTTTACTTATCTTTCATTTTACCACGACTCTAGATCTTTGAATTGTTTGTTTTCTTGCCAAAAGCTTATGTCACATCCAGAGGTTTACGAGGGATATGTCCCGATGGCGTATGTTGATTACTTGGAGAAGATGTCCAGGTTCTAACTGATTTCTTTTCTTTTTCTACCTTACTCTCCTTCAAAACACCATGTTCACCTTTTACTTCTATGAGGCAGGAACGGTGAATGGGGTGATCATGTCACATTACAGGCAGCCGTGGATTCGGTAAGGTCTACATAGAATTCACTGCCTAAATTTTCTTTCAAATGAGGTTTACAGCATTTTGTTTTAGTAAGTAACGGTCTAAGCTCACTGCTAGCAGATACTGTCCTTTTTGGGCTTTCGCCTTCCGTGCTTCCTTCAAGGTTTTCAAACGCGTCTGCTAGGGAGAAGTTTCCACACCCTTATAAAGAATGTTTTGTTTCCCCCTCCAACCGATGTGGGATCTCACAATCGACCCCTTCGGGGCCAACGTCTACGCTGACACTCGTTCCTCTCTCCAATTGATGTCCGGGACACCCCCCGCCCCCCCCCGGAATCCACCCCCTTCAGGGCCCAGCATCCTCGATGACACCCGCCTAGTGTCCACCCCCATTTGGGGCTAGCCTCCTCGCTGGCACACTGCTCAGTGTCTAGCTCTGATACCATTTGTAACGACCCAAGTCCACTGCCAGCAGATATTGTCTTCTTTAGGATTTTCCTTCCGGGCTTCCCCTCAAAGTTTTAAAACGTGTCTGTTAGGCAGAGGTTTCTACACCTTTACAAAGAATGCTTTGTTCCCCTCTCCAACCGAGGTGGGATCTCACATGGTGTCACTCTATTCTAGATAATTTCCTTTGGTTAACTCCTACGGGGGTTGAAGGCAACGATAATATCTTATCATTCCTCGTTAAGGAAATCAATCTTGCCTTTTGGTTATCTCCCTATCTGTTGCTCCAATACTTTAATTTCAGCTACTGAGCTTACAATCACTGAAGTTAGTTACATGTGAACTGCATCCTTGTCTTGACATCATAATCATCTTCTGATGCAGTATGGCGTGCGAATATTCGTTATAACTTCTTTCAAGGATACCTGCTGCATAGAGATTCTTCCAAATTCTCAGAAGACAAAAGGAGGTAAACAATACATATTTGCAGATGACTGGTGATCTTATTATTAGCCTTATTTTAACTCTTTCCATCCCCCTTTGTTTTTTCTTCTTCTTTCATTTGAATGTTGCGTTTCTAAAATTGTCTTATTGTTTACCCCTTCAATGGTTCACCATCGAACTTTATCTCGTTCGTTGTTTTTATTTGCCGTTCTTCATTATCTTTAAGTTTAATTTGCATCGATACATGGCTCAAGTCCCGTTTCCATTTTTCTTCCCTGTCGATATTATTGCCTCTGATTGTAGCTATACCATGCGTTCGCAGTAATTTTCTTGAGTTTTTGGGCAGAGGTGCATTACAACTCAATTCATCCTCAGGGAGGTAAATACTGTTCCCAACTTTGTATTGTGTTTGCCATTGCTTGAAATAGGGGTATGATAAATAGCTAAGAGCTAAGCCTAGAAAATGGGAGGAAAAGAAAGAGGACTTATTTGCTTTAGCCAAACTAAGTTGTAAGTAACCTTGGCTCTTCGAGGGTATTCTTGCTTAGGAGATGCCTAATTTATTTTAGGTAAAAAGAAAGAAACACTCTCTTCTTCCGTTCGTTTGTTTTTCTAATGTACTAAGTTGCCTATTAGGATCTTTCTGTTGAAGATTGTTGGGAGAAGAGTCCTGGTTATGAGTTTATAAGTAAGGAATATTATCTCCATTGGTACGAGGTTTTTTTAGAAAACTAAAAGGCCATGAGAACTTATGCTCAAAGTAGACAATACCATACCATTGTGGAGGAACGGGATTCCTAACACTTTCAATTGGCTCAATTGACTTTGACAAGAATGATTTAGAGCGTCAAGCCATTCACTTAATAGCATGTTTGAGCTTAGCTTTGGTCCAACTCGATCTCGGTGCTTAAAATTTTATGATGTTTTGCTATACATTCAGGTATGCCATCAACCGGAGATTCCCCACCCTGTGAGTTGAGAAAGAAGAAAAGATGGTGGAAATTTGGAAATAAGCATTGAAAAGCCCTGCTGCCTCAGACTCCTAATGGTTTGTCTGCTGCTATTACTGAACTTCTATTGCTCAAATGCATTCCTTTGTTAGTGGCTAACATTACACATGCCTTGAATTAGTTAATGACAAAATAAATTGACTCTTCATTCTTTCTGTTGTCCCCTCCATAAAAGCTTAAGCAGGGTTGTTTGCCTGTGTTAGTTTGGTTTAATTGGAAGGGATTTTGAGGGAGAAACGGATTGTAATGTGTTATTCTTTTGATAGAAATGCAAGAGCATCGAGGACCTTTACTCTTCGAAAGCGTTGAGTTTATCGTTTATCGTTGTCATTAATTGAAATCGTTTATTGTTTATCGTAATCA

mRNA sequence

ACGATTTTTCAATCAAAAGCCGCAAAGGGTCGGCCACAACCAACCCAACAAATCTCAGTCGCTTGATTTTCCACCATCCATTCGAAATCCAATCTCATCCGTTCTTCGCCGCCTCCGTTCTTTGACCATCGCCGATCTCCACCGTCAAATCCGACCTCTGAAATTAACACCCATGATTTCTTGATTACGGGGTTTATCATTCTTTTTTTCTTAAACTAGAATCCCATCACATCAATCGTTGAAGTATTAGCATGAAACTTGGCAGCCATAGCCAGTTCTTTTGAACAAACTCGCTCGAAAGGGCGTTTAAACGGCTCGAAAGGGCGACTATACTGTATAGATAGATGTATATGGATTCATATGGCATGAGTTTCGACTGTTTGTTTTAATTTATTGATTTCTGTTCTTTTGCCCATTTTCATTTTCAATGCGTTCATCGTCATCCTCGGTGCTGATTAACTTTCCGTAATTGCTCCCTGACCTGTGAACCTCTTATTTCCTGTTCTACCCACCTTGTTCATTCGAGCTAAAACGGAGTTTTCGTTCGTGGCTGCGAATTCAAGGTAATTTGTGACTGGAAATTGGACGTATATGCTTCGATTATGTCGAACTTGTTCTTGTTTATTGAATCGCCAGGAAAGGATTACACATATGCATTCATTTCTTGAAAACATCTTGAAATTCTTACTTGTTTTTGTATCTAGATGGTTCCTTCTGGTTATGAAGCGGATGTTATTGGGTGGGGTCGTCGTCTTTTTGATGATGATTCAGTTTTTAATTCTGGGTATTACGGTGAGATGACAACAGTAGATGACCATTACCCTGGGAATTATTACAGAGATCATTACAATTTAGAACAGAATTCCGTGGAGAATGATGAGATTATTGCTAGAACTCTGCAAGAAAACTTGTCACAGTTATCAATTACAGACTCCTCTAGATACCCAGTTGAAAGAGAGGAGCCACTGCAAGGATCCATGTATACAATTGGTTGGCATAATTCATTTCCTAGGAATAACAATTCAGAGAGTATTTCTTCTCCTGAGGAAGATTTTGAAACTCATGATCCTCCCAGTTCATGTTCTAGTCTTGGGGATGAGAACTTCTATTCACATGCTGTTGATGGTGAAGAACTCTGGAGATTCAATCAAATGATTCCTGTTCCTGTAGACTCTAATTTGTTTGGCGCTTCGCCTCTTTACATGTTCTGTACTTGCAGAGTCTTGGAGATGCATGTTCCTAGAATTAACGGAGAGATTCCTTCAGTTGATGAAGCTGCTTCTGATCATGAAAGGCTGCTGAACAGATTACAAGTGTATGACTTTGTTGAACGAAAGGTTCAAGGTGATGGCAATTGTCAGTTTCGTGCATTATCTGATCAATTATATGGAACACCTGACAATCACGAACTCGTGAGACAGAAAGTTGTAGATCAGCTTATGTCACATCCAGAGGTTTACGAGGGATATGTCCCGATGGCGTATGTTGATTACTTGGAGAAGATGTCCAGGAACGGTGAATGGGGTGATCATGTCACATTACAGGCAGCCAGGTGCATTACAACTCAATTCATCCTCAGGGAGGTATGCCATCAACCGGAGATTCCCCACCCTGTGAGTTGAGAAAGAAGAAAAGATGGTGGAAATTTGGAAATAAGCATTGAAAAGCCCTGCTGCCTCAGACTCCTAATGGTTTGTCTGCTGCTATTACTGAACTTCTATTGCTCAAATGCATTCCTTTGTTAGTGGCTAACATTACACATGCCTTGAATTAGTTAATGACAAAATAAATTGACTCTTCATTCTTTCTGTTGTCCCCTCCATAAAAGCTTAAGCAGGGTTGTTTGCCTGTGTTAGTTTGGTTTAATTGGAAGGGATTTTGAGGGAGAAACGGATTGTAATGTGTTATTCTTTTGATAGAAATGCAAGAGCATCGAGGACCTTTACTCTTCGAAAGCGTTGAGTTTATCGTTTATCGTTGTCATTAATTGAAATCGTTTATTGTTTATCGTAATCA

Coding sequence (CDS)

ATGGTTCCTTCTGGTTATGAAGCGGATGTTATTGGGTGGGGTCGTCGTCTTTTTGATGATGATTCAGTTTTTAATTCTGGGTATTACGGTGAGATGACAACAGTAGATGACCATTACCCTGGGAATTATTACAGAGATCATTACAATTTAGAACAGAATTCCGTGGAGAATGATGAGATTATTGCTAGAACTCTGCAAGAAAACTTGTCACAGTTATCAATTACAGACTCCTCTAGATACCCAGTTGAAAGAGAGGAGCCACTGCAAGGATCCATGTATACAATTGGTTGGCATAATTCATTTCCTAGGAATAACAATTCAGAGAGTATTTCTTCTCCTGAGGAAGATTTTGAAACTCATGATCCTCCCAGTTCATGTTCTAGTCTTGGGGATGAGAACTTCTATTCACATGCTGTTGATGGTGAAGAACTCTGGAGATTCAATCAAATGATTCCTGTTCCTGTAGACTCTAATTTGTTTGGCGCTTCGCCTCTTTACATGTTCTGTACTTGCAGAGTCTTGGAGATGCATGTTCCTAGAATTAACGGAGAGATTCCTTCAGTTGATGAAGCTGCTTCTGATCATGAAAGGCTGCTGAACAGATTACAAGTGTATGACTTTGTTGAACGAAAGGTTCAAGGTGATGGCAATTGTCAGTTTCGTGCATTATCTGATCAATTATATGGAACACCTGACAATCACGAACTCGTGAGACAGAAAGTTGTAGATCAGCTTATGTCACATCCAGAGGTTTACGAGGGATATGTCCCGATGGCGTATGTTGATTACTTGGAGAAGATGTCCAGGAACGGTGAATGGGGTGATCATGTCACATTACAGGCAGCCAGGTGCATTACAACTCAATTCATCCTCAGGGAGGTATGCCATCAACCGGAGATTCCCCACCCTGTGAGTTGA

Protein sequence

MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEIIARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETHDPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAARCITTQFILREVCHQPEIPHPVS
Homology
BLAST of Carg00973 vs. NCBI nr
Match: KAG7028027.1 (OTU domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 643.7 bits (1659), Expect = 8.3e-181
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI
Sbjct: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120
           IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH
Sbjct: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120

Query: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPR 180
           DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPR
Sbjct: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPR 180

Query: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240
           INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK
Sbjct: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240

Query: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAARCITTQFILREVCHQPEI 300
           VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAARCITTQFILREVCHQPEI
Sbjct: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAARCITTQFILREVCHQPEI 300

Query: 301 PHPVS 306
           PHPVS
Sbjct: 301 PHPVS 305

BLAST of Carg00973 vs. NCBI nr
Match: KAG6596479.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 533.5 bits (1373), Expect = 1.2e-147
Identity = 260/282 (92.20%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI
Sbjct: 30  MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 89

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120
           IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH
Sbjct: 90  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 149

Query: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPR 180
           DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP                      HVPR
Sbjct: 150 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP----------------------HVPR 209

Query: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240
           INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK
Sbjct: 210 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 269

Query: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA
Sbjct: 270 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 289

BLAST of Carg00973 vs. NCBI nr
Match: XP_022949140.1 (uncharacterized protein LOC111452572 isoform X2 [Cucurbita moschata])

HSP 1 Score: 531.6 bits (1368), Expect = 4.6e-147
Identity = 259/282 (91.84%), Postives = 259/282 (91.84%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLE NSVENDEI
Sbjct: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEHNSVENDEI 60

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120
           IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH
Sbjct: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120

Query: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPR 180
           DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP                      HVPR
Sbjct: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP----------------------HVPR 180

Query: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240
           INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK
Sbjct: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240

Query: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA
Sbjct: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 260

BLAST of Carg00973 vs. NCBI nr
Match: XP_023540725.1 (uncharacterized protein LOC111801006 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 530.4 bits (1365), Expect = 1.0e-146
Identity = 258/282 (91.49%), Postives = 259/282 (91.84%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLE NSVENDEI
Sbjct: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEHNSVENDEI 60

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120
           IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH
Sbjct: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120

Query: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPR 180
           DPP+SCSSLGDENFYSHAVDGEELWRFNQMIPVP                      HVPR
Sbjct: 121 DPPTSCSSLGDENFYSHAVDGEELWRFNQMIPVP----------------------HVPR 180

Query: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240
           INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK
Sbjct: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240

Query: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA
Sbjct: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 260

BLAST of Carg00973 vs. NCBI nr
Match: XP_023005716.1 (uncharacterized protein LOC111498629 isoform X2 [Cucurbita maxima])

HSP 1 Score: 527.3 bits (1357), Expect = 8.6e-146
Identity = 258/282 (91.49%), Postives = 258/282 (91.49%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI
Sbjct: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120
           IARTLQENLSQLSITDSSRYPVEREEPLQ SMYTIGW NSFPRNNNSESISSPEEDFETH
Sbjct: 61  IARTLQENLSQLSITDSSRYPVEREEPLQESMYTIGWQNSFPRNNNSESISSPEEDFETH 120

Query: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPR 180
           DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP                      HVPR
Sbjct: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP----------------------HVPR 180

Query: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240
           INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK
Sbjct: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240

Query: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA
Sbjct: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 260

BLAST of Carg00973 vs. ExPASy Swiss-Prot
Match: Q9LZF7 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10 OS=Arabidopsis thaliana OX=3702 GN=OTU10 PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.9e-51
Identity = 119/269 (44.24%), Postives = 159/269 (59.11%), Query Frame = 0

Query: 38  HYPGNYYRDHYNLEQNSVENDEIIARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGW 97
           H  G YYR++ +   + V+NDEIIARTLQ++  QL I +S+ Y  + +   Q      G+
Sbjct: 45  HQSGEYYREYEDHRSSDVDNDEIIARTLQDDFLQLEIAESNDYSHQNQ---QQQHQQEGY 104

Query: 98  HNSFPRNNN--------------SESISSP-EEDFETHDPPS--SCSSLGDENFYSHA-- 157
            N++  NNN              SE I +  ++D  + D  +  SCSS  D + Y ++  
Sbjct: 105 TNNYSNNNNGYAWNDQSPAVDYSSEWIGNDNDQDGRSDDSVNVFSCSSPSDTDEYVYSWE 164

Query: 158 -----VDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPRINGEIPSVDEAAS 217
                 DGE   R NQM+P+P                      ++P+INGEIP  +EA S
Sbjct: 165 SDQCDADGEFGRRLNQMVPIP----------------------YIPKINGEIPPEEEAVS 224

Query: 218 DHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQKVVDQLMSHPEVYE 277
           DHERL NRL+++DF E KV GDGNCQFRAL+DQLY T D H+ VR+++V QL S P+ Y+
Sbjct: 225 DHERLRNRLEMFDFTEVKVPGDGNCQFRALADQLYKTADRHKHVRRQIVKQLKSRPDSYQ 284

Query: 278 GYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           GYVPM + DYL KMSR+GEWGDHVTLQAA
Sbjct: 285 GYVPMDFSDYLRKMSRSGEWGDHVTLQAA 288

BLAST of Carg00973 vs. ExPASy Swiss-Prot
Match: Q8LBW2 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 9 OS=Arabidopsis thaliana OX=3702 GN=OTU9 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.3e-45
Identity = 123/330 (37.27%), Postives = 173/330 (52.42%), Query Frame = 0

Query: 5   GYE--ADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPG--NYYRDHYNLEQNS-VENDE 64
           GYE   D + WG    +  ++ N+G    +T  +    G   Y R+ YN      V+ND 
Sbjct: 2   GYEPDPDALRWGLHDLEVCTLTNAGSCSSVTRYESGGGGTQGYVREGYNQPVTGYVDNDA 61

Query: 65  IIARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGW-HNSFPRNNNSESISSPEEDFE 124
           +IA+  Q+ LS+++  ++S   +    P   S+    W H    + N  E+I   +E   
Sbjct: 62  VIAQFYQDELSRVARAEAS--GINSLSPT--SVVAQDWPHPHQGQENQGEAIDITQESDI 121

Query: 125 TH----------------DPPSSCSSLGDENFYSHAVDGEELW-----RFNQMIPVPVDS 184
            H                +   S  S  D++  S  ++ EE W     R NQMIP+    
Sbjct: 122 LHNHNGNMEDKNVARIRFEGGQSSPSRDDDSVCSVEIE-EESWSEVGKRLNQMIPI---- 181

Query: 185 NLFGASPLYMFCTCRVLEMHVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGN 244
                              HVP+INGE+PS DE  SDHERL  RLQ+Y  VE K++GDGN
Sbjct: 182 ------------------AHVPKINGELPSEDEQISDHERLFQRLQLYGLVENKIEGDGN 241

Query: 245 CQFRALSDQLYGTPDNHELVRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHV 303
           CQFR+LSDQLY +P++H  VR++VV+QL  + E+YEGYVPMAY DYL+ M RNGEWGDHV
Sbjct: 242 CQFRSLSDQLYRSPEHHNFVREQVVNQLAYNREIYEGYVPMAYNDYLKAMKRNGEWGDHV 301

BLAST of Carg00973 vs. ExPASy Swiss-Prot
Match: Q9SGA5 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 OS=Arabidopsis thaliana OX=3702 GN=OTU12 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-33
Identity = 72/132 (54.55%), Postives = 88/132 (66.67%), Query Frame = 0

Query: 177 HVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHEL 236
           HVPRIN  IP++++A  DH+RLL RL VY   E KV GDGNCQFRALSDQLY +P+ H+ 
Sbjct: 49  HVPRINCYIPNLNDATLDHQRLLQRLNVYGLCELKVSGDGNCQFRALSDQLYRSPEYHKQ 108

Query: 237 VRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAAR-------CITTQF 296
           VR++VV QL     +YE YVPM Y  Y +KM + GEWGDH+TLQAA        C+ T F
Sbjct: 109 VRREVVKQLKECRSMYESYVPMKYKRYYKKMGKFGEWGDHITLQAAADRFAAKICLLTSF 168

Query: 297 ILREVCHQPEIP 302
             R+ C    IP
Sbjct: 169 --RDTCFIEIIP 178

BLAST of Carg00973 vs. ExPASy Swiss-Prot
Match: Q0V869 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 11 OS=Arabidopsis thaliana OX=3702 GN=OTU11 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.1e-30
Identity = 65/133 (48.87%), Postives = 84/133 (63.16%), Query Frame = 0

Query: 177 HVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHEL 236
           H PR+N EIP +++A  DHE L  RL  Y   E +++GDGNCQFRAL+DQL+   D H+ 
Sbjct: 71  HTPRVNREIPDINDATLDHELLSGRLATYGLAELQMEGDGNCQFRALADQLFRNADYHKH 130

Query: 237 VRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAAR-------CITTQF 296
           VR+ VV QL    ++YE YVPM Y  Y  KM ++GEWGDHVTLQAA        C+ T F
Sbjct: 131 VRKHVVKQLKQQRKLYEEYVPMKYRHYTRKMKKHGEWGDHVTLQAAADRFEAKICLVTSF 190

Query: 297 ILREVCHQPEIPH 303
             R+  +   +PH
Sbjct: 191 --RDQSYIEILPH 201

BLAST of Carg00973 vs. ExPASy Swiss-Prot
Match: Q54P70 (OTU domain-containing protein DDB_G0284757 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0284757 PE=4 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 1.5e-12
Identity = 43/107 (40.19%), Postives = 58/107 (54.21%), Query Frame = 0

Query: 185 IPSVDEAASDHERLLNRLQVYDFVERK-VQGDGNCQFRALSDQLYGTPDNHELVRQKVVD 244
           +P   E     +RL  RL++Y     K + GDGNCQ  ALSDQLYG   + + VR+ +VD
Sbjct: 608 LPQSKEVQIAQQRLNERLELYMLKNSKEIPGDGNCQMHALSDQLYGDLSHSQEVRKTIVD 667

Query: 245 QLMSHP--EVYEGYVPMAYV------DYLEKMSRNGEWGDHVTLQAA 283
            L  +   ++  G     +V      DY   MS+NG WGDH+TL AA
Sbjct: 668 WLRKNKDFQLPNGATICQFVNTNNWDDYCNDMSKNGNWGDHLTLLAA 714

BLAST of Carg00973 vs. ExPASy TrEMBL
Match: A0A6J1GBY2 (uncharacterized protein LOC111452572 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452572 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.2e-147
Identity = 259/282 (91.84%), Postives = 259/282 (91.84%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLE NSVENDEI
Sbjct: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEHNSVENDEI 60

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120
           IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH
Sbjct: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120

Query: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPR 180
           DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP                      HVPR
Sbjct: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP----------------------HVPR 180

Query: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240
           INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK
Sbjct: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240

Query: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA
Sbjct: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 260

BLAST of Carg00973 vs. ExPASy TrEMBL
Match: A0A6J1KVS5 (uncharacterized protein LOC111498629 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498629 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 4.2e-146
Identity = 258/282 (91.49%), Postives = 258/282 (91.49%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI
Sbjct: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120
           IARTLQENLSQLSITDSSRYPVEREEPLQ SMYTIGW NSFPRNNNSESISSPEEDFETH
Sbjct: 61  IARTLQENLSQLSITDSSRYPVEREEPLQESMYTIGWQNSFPRNNNSESISSPEEDFETH 120

Query: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPR 180
           DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP                      HVPR
Sbjct: 121 DPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP----------------------HVPR 180

Query: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240
           INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK
Sbjct: 181 INGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQK 240

Query: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA
Sbjct: 241 VVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 260

BLAST of Carg00973 vs. ExPASy TrEMBL
Match: A0A6J1GBX3 (uncharacterized protein LOC111452572 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452572 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.2e-145
Identity = 259/286 (90.56%), Postives = 259/286 (90.56%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLE NSVENDEI
Sbjct: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEHNSVENDEI 60

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNS----ESISSPEED 120
           IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNS    ESISSPEED
Sbjct: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSVPIVESISSPEED 120

Query: 121 FETHDPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEM 180
           FETHDPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP                      
Sbjct: 121 FETHDPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP---------------------- 180

Query: 181 HVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHEL 240
           HVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHEL
Sbjct: 181 HVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHEL 240

Query: 241 VRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           VRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA
Sbjct: 241 VRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 264

BLAST of Carg00973 vs. ExPASy TrEMBL
Match: A0A6J1KY63 (uncharacterized protein LOC111498629 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498629 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.3e-144
Identity = 258/286 (90.21%), Postives = 258/286 (90.21%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI
Sbjct: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNS----ESISSPEED 120
           IARTLQENLSQLSITDSSRYPVEREEPLQ SMYTIGW NSFPRNNNS    ESISSPEED
Sbjct: 61  IARTLQENLSQLSITDSSRYPVEREEPLQESMYTIGWQNSFPRNNNSVLIVESISSPEED 120

Query: 121 FETHDPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEM 180
           FETHDPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP                      
Sbjct: 121 FETHDPPSSCSSLGDENFYSHAVDGEELWRFNQMIPVP---------------------- 180

Query: 181 HVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHEL 240
           HVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHEL
Sbjct: 181 HVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHEL 240

Query: 241 VRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           VRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA
Sbjct: 241 VRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 264

BLAST of Carg00973 vs. ExPASy TrEMBL
Match: A0A0A0LAZ3 (OTU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G740120 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.4e-120
Identity = 220/283 (77.74%), Postives = 234/283 (82.69%), Query Frame = 0

Query: 1   MVPSGYEADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPGNYYRDHYNLEQNSVENDEI 60
           MVPSGYE+DVI WG RLFD DSVFNSGYYGEMT VDDHYPGNYYRDHYNLE   VENDEI
Sbjct: 1   MVPSGYESDVIQWGLRLFDGDSVFNSGYYGEMTAVDDHYPGNYYRDHYNLEHTCVENDEI 60

Query: 61  IARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGWHNSFPRNNNSESISSPEEDFETH 120
           IARTLQENLS LSITDSSR P ERE+ L+G +YT  W+N FPR+NNS    S EED ET 
Sbjct: 61  IARTLQENLSHLSITDSSRCPFEREDQLRGPIYTTAWNNPFPRSNNSSESISVEEDIETM 120

Query: 121 DPPSSCSSLGDENF-YSHAVDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVP 180
           DP SSCSS GDE+F Y +A+DGEELWRFNQ+IPVP                      HVP
Sbjct: 121 DPSSSCSSPGDEDFSYLYALDGEELWRFNQVIPVP----------------------HVP 180

Query: 181 RINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQ 240
           RINGEIPSVDEAASDHERLL+RLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQ
Sbjct: 181 RINGEIPSVDEAASDHERLLDRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQ 240

Query: 241 KVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           KVV+QLMSHPE+YEGYVPMAY +YLEKMSRNGEWGDHVTLQAA
Sbjct: 241 KVVNQLMSHPEIYEGYVPMAYDEYLEKMSRNGEWGDHVTLQAA 261

BLAST of Carg00973 vs. TAIR 10
Match: AT5G03330.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-52
Identity = 119/269 (44.24%), Postives = 159/269 (59.11%), Query Frame = 0

Query: 38  HYPGNYYRDHYNLEQNSVENDEIIARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGW 97
           H  G YYR++ +   + V+NDEIIARTLQ++  QL I +S+ Y  + +   Q      G+
Sbjct: 45  HQSGEYYREYEDHRSSDVDNDEIIARTLQDDFLQLEIAESNDYSHQNQ---QQQHQQEGY 104

Query: 98  HNSFPRNNN--------------SESISSP-EEDFETHDPPS--SCSSLGDENFYSHA-- 157
            N++  NNN              SE I +  ++D  + D  +  SCSS  D + Y ++  
Sbjct: 105 TNNYSNNNNGYAWNDQSPAVDYSSEWIGNDNDQDGRSDDSVNVFSCSSPSDTDEYVYSWE 164

Query: 158 -----VDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPRINGEIPSVDEAAS 217
                 DGE   R NQM+P+P                      ++P+INGEIP  +EA S
Sbjct: 165 SDQCDADGEFGRRLNQMVPIP----------------------YIPKINGEIPPEEEAVS 224

Query: 218 DHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQKVVDQLMSHPEVYE 277
           DHERL NRL+++DF E KV GDGNCQFRAL+DQLY T D H+ VR+++V QL S P+ Y+
Sbjct: 225 DHERLRNRLEMFDFTEVKVPGDGNCQFRALADQLYKTADRHKHVRRQIVKQLKSRPDSYQ 284

Query: 278 GYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           GYVPM + DYL KMSR+GEWGDHVTLQAA
Sbjct: 285 GYVPMDFSDYLRKMSRSGEWGDHVTLQAA 288

BLAST of Carg00973 vs. TAIR 10
Match: AT5G03330.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-52
Identity = 119/269 (44.24%), Postives = 159/269 (59.11%), Query Frame = 0

Query: 38  HYPGNYYRDHYNLEQNSVENDEIIARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGW 97
           H  G YYR++ +   + V+NDEIIARTLQ++  QL I +S+ Y  + +   Q      G+
Sbjct: 45  HQSGEYYREYEDHRSSDVDNDEIIARTLQDDFLQLEIAESNDYSHQNQ---QQQHQQEGY 104

Query: 98  HNSFPRNNN--------------SESISSP-EEDFETHDPPS--SCSSLGDENFYSHA-- 157
            N++  NNN              SE I +  ++D  + D  +  SCSS  D + Y ++  
Sbjct: 105 TNNYSNNNNGYAWNDQSPAVDYSSEWIGNDNDQDGRSDDSVNVFSCSSPSDTDEYVYSWE 164

Query: 158 -----VDGEELWRFNQMIPVPVDSNLFGASPLYMFCTCRVLEMHVPRINGEIPSVDEAAS 217
                 DGE   R NQM+P+P                      ++P+INGEIP  +EA S
Sbjct: 165 SDQCDADGEFGRRLNQMVPIP----------------------YIPKINGEIPPEEEAVS 224

Query: 218 DHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHELVRQKVVDQLMSHPEVYE 277
           DHERL NRL+++DF E KV GDGNCQFRAL+DQLY T D H+ VR+++V QL S P+ Y+
Sbjct: 225 DHERLRNRLEMFDFTEVKVPGDGNCQFRALADQLYKTADRHKHVRRQIVKQLKSRPDSYQ 284

Query: 278 GYVPMAYVDYLEKMSRNGEWGDHVTLQAA 283
           GYVPM + DYL KMSR+GEWGDHVTLQAA
Sbjct: 285 GYVPMDFSDYLRKMSRSGEWGDHVTLQAA 288

BLAST of Carg00973 vs. TAIR 10
Match: AT5G04250.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.7e-46
Identity = 123/330 (37.27%), Postives = 173/330 (52.42%), Query Frame = 0

Query: 5   GYE--ADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPG--NYYRDHYNLEQNS-VENDE 64
           GYE   D + WG    +  ++ N+G    +T  +    G   Y R+ YN      V+ND 
Sbjct: 2   GYEPDPDALRWGLHDLEVCTLTNAGSCSSVTRYESGGGGTQGYVREGYNQPVTGYVDNDA 61

Query: 65  IIARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGW-HNSFPRNNNSESISSPEEDFE 124
           +IA+  Q+ LS+++  ++S   +    P   S+    W H    + N  E+I   +E   
Sbjct: 62  VIAQFYQDELSRVARAEAS--GINSLSPT--SVVAQDWPHPHQGQENQGEAIDITQESDI 121

Query: 125 TH----------------DPPSSCSSLGDENFYSHAVDGEELW-----RFNQMIPVPVDS 184
            H                +   S  S  D++  S  ++ EE W     R NQMIP+    
Sbjct: 122 LHNHNGNMEDKNVARIRFEGGQSSPSRDDDSVCSVEIE-EESWSEVGKRLNQMIPI---- 181

Query: 185 NLFGASPLYMFCTCRVLEMHVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGN 244
                              HVP+INGE+PS DE  SDHERL  RLQ+Y  VE K++GDGN
Sbjct: 182 ------------------AHVPKINGELPSEDEQISDHERLFQRLQLYGLVENKIEGDGN 241

Query: 245 CQFRALSDQLYGTPDNHELVRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHV 303
           CQFR+LSDQLY +P++H  VR++VV+QL  + E+YEGYVPMAY DYL+ M RNGEWGDHV
Sbjct: 242 CQFRSLSDQLYRSPEHHNFVREQVVNQLAYNREIYEGYVPMAYNDYLKAMKRNGEWGDHV 301

BLAST of Carg00973 vs. TAIR 10
Match: AT5G04250.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.7e-46
Identity = 123/330 (37.27%), Postives = 173/330 (52.42%), Query Frame = 0

Query: 5   GYE--ADVIGWGRRLFDDDSVFNSGYYGEMTTVDDHYPG--NYYRDHYNLEQNS-VENDE 64
           GYE   D + WG    +  ++ N+G    +T  +    G   Y R+ YN      V+ND 
Sbjct: 2   GYEPDPDALRWGLHDLEVCTLTNAGSCSSVTRYESGGGGTQGYVREGYNQPVTGYVDNDA 61

Query: 65  IIARTLQENLSQLSITDSSRYPVEREEPLQGSMYTIGW-HNSFPRNNNSESISSPEEDFE 124
           +IA+  Q+ LS+++  ++S   +    P   S+    W H    + N  E+I   +E   
Sbjct: 62  VIAQFYQDELSRVARAEAS--GINSLSPT--SVVAQDWPHPHQGQENQGEAIDITQESDI 121

Query: 125 TH----------------DPPSSCSSLGDENFYSHAVDGEELW-----RFNQMIPVPVDS 184
            H                +   S  S  D++  S  ++ EE W     R NQMIP+    
Sbjct: 122 LHNHNGNMEDKNVARIRFEGGQSSPSRDDDSVCSVEIE-EESWSEVGKRLNQMIPI---- 181

Query: 185 NLFGASPLYMFCTCRVLEMHVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGN 244
                              HVP+INGE+PS DE  SDHERL  RLQ+Y  VE K++GDGN
Sbjct: 182 ------------------AHVPKINGELPSEDEQISDHERLFQRLQLYGLVENKIEGDGN 241

Query: 245 CQFRALSDQLYGTPDNHELVRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHV 303
           CQFR+LSDQLY +P++H  VR++VV+QL  + E+YEGYVPMAY DYL+ M RNGEWGDHV
Sbjct: 242 CQFRSLSDQLYRSPEHHNFVREQVVNQLAYNREIYEGYVPMAYNDYLKAMKRNGEWGDHV 301

BLAST of Carg00973 vs. TAIR 10
Match: AT3G02070.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.5e-34
Identity = 72/132 (54.55%), Postives = 88/132 (66.67%), Query Frame = 0

Query: 177 HVPRINGEIPSVDEAASDHERLLNRLQVYDFVERKVQGDGNCQFRALSDQLYGTPDNHEL 236
           HVPRIN  IP++++A  DH+RLL RL VY   E KV GDGNCQFRALSDQLY +P+ H+ 
Sbjct: 49  HVPRINCYIPNLNDATLDHQRLLQRLNVYGLCELKVSGDGNCQFRALSDQLYRSPEYHKQ 108

Query: 237 VRQKVVDQLMSHPEVYEGYVPMAYVDYLEKMSRNGEWGDHVTLQAAR-------CITTQF 296
           VR++VV QL     +YE YVPM Y  Y +KM + GEWGDH+TLQAA        C+ T F
Sbjct: 109 VRREVVKQLKECRSMYESYVPMKYKRYYKKMGKFGEWGDHITLQAAADRFAAKICLLTSF 168

Query: 297 ILREVCHQPEIP 302
             R+ C    IP
Sbjct: 169 --RDTCFIEIIP 178

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028027.18.3e-181100.00OTU domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6596479.11.2e-14792.20OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10, partial [Cucurbita a... [more]
XP_022949140.14.6e-14791.84uncharacterized protein LOC111452572 isoform X2 [Cucurbita moschata][more]
XP_023540725.11.0e-14691.49uncharacterized protein LOC111801006 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023005716.18.6e-14691.49uncharacterized protein LOC111498629 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LZF72.9e-5144.24OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10 OS=Arabidopsis thalia... [more]
Q8LBW22.3e-4537.27OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 9 OS=Arabidopsis thalian... [more]
Q9SGA52.1e-3354.55OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 OS=Arabidopsis thalia... [more]
Q0V8692.1e-3048.87OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 11 OS=Arabidopsis thalia... [more]
Q54P701.5e-1240.19OTU domain-containing protein DDB_G0284757 OS=Dictyostelium discoideum OX=44689 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GBY22.2e-14791.84uncharacterized protein LOC111452572 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KVS54.2e-14691.49uncharacterized protein LOC111498629 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1GBX31.2e-14590.56uncharacterized protein LOC111452572 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KY632.3e-14490.21uncharacterized protein LOC111498629 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0LAZ31.4e-12077.74OTU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G740120 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT5G03330.12.0e-5244.24Cysteine proteinases superfamily protein [more]
AT5G03330.22.0e-5244.24Cysteine proteinases superfamily protein [more]
AT5G04250.11.7e-4637.27Cysteine proteinases superfamily protein [more]
AT5G04250.21.7e-4637.27Cysteine proteinases superfamily protein [more]
AT3G02070.11.5e-3454.55Cysteine proteinases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003323OTU domainPFAMPF02338OTUcoord: 213..282
e-value: 7.9E-8
score: 32.8
IPR003323OTU domainPROSITEPS50802OTUcoord: 207..305
score: 10.678013
NoneNo IPR availableGENE3D3.90.70.80coord: 188..293
e-value: 2.3E-28
score: 100.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 104..129
NoneNo IPR availablePANTHERPTHR12419OTU DOMAIN CONTAINING PROTEINcoord: 177..282
coord: 33..154
NoneNo IPR availablePANTHERPTHR12419:SF78OTU DOMAIN-CONTAINING PROTEIN DDB_G0284757-LIKE ISOFORM X1coord: 177..282
coord: 33..154
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 194..282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00973-RACarg00973-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016579 protein deubiquitination
molecular_function GO:0004843 thiol-dependent deubiquitinase