Carg00929 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00929
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	delta(14)-sterol reductase-like
Location	Carg_Chr06: 2078836 .. 2084709 (+)
RNA-Seq Expression	Carg00929
Synteny	Carg00929

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATTTACAAGTTAGCAGAGCAGTTGAACCAGCGCCCGTGGATTTAGTAGCTGCAATGAACAGACTCGGGGCTCGAGCTAAAGGCAGCCATAGTTGAAATTAGACAATCCCGAGAAGAAATTAGTTCCAATTTTGCAGCAAATTATCATTGTTACCAGAGGAAACAAGTCGATCGAGCGACAAAATCCGAACAAGTTGTCAATGATCGATTTCTTGAGGAATATGGATCTGGAGTTTCTTCTTCAACTTGCAATCCCTTCTTGGGAAGCTGTAAGTGCTAATTCTTTTGTATAAGTTTGATTTATACTTCAATGTGTTTGAGATTCTTCCTGCTGTATGTTGTTGGATAATCAGGTTGCTATACTTTCCATTTTCTTCTTTTACTTGGCGATTGCTGGCTCTGTTCTACCTGGAAGGATTATCCCAGGAGTGATCTTGTCGGACCGCACTAGGCTTCATTATCGCTGCAATGGTATCGTTATCTCATATGTAAATGCAGTTTGTTAAGTTTGATATCACTGAGATCATCATTTCTTGGCAAATTAGTACTAAAATTTGGATTCCTCTTTGGGTTCTTTTGTGCTGCTATAGGTCTACTCTCTCTTCTGGTGCTTGTTGCACTTCTTGGGATTGGTGTTAAGATGAATTACATTTCGCCCACTGTGAGTGTTCATTGATTTTCGCATGTGTTGGATCATTCTACTTATTTGGCTCTACCGAAATTTTTTTATTGAAATTTTGTATTAGTATTTATTCCTTGATGTTGGTAACTGTCCTTATTAGATGCTTATAATACAACTCATATGTAGGCAATTTCAGATAGAGGGGTGGAGCTGTTATCCGCTACCTTCTGGTTTAGTGTCCTGGTATGTGAAATTTCAAATTGAATATCTGAACTATTTGCTATGCAGCTGATATTATCAACGTTACTTGCTGACATTTTTCTGCATCGAACTTTTGCACATCAACATCCCTGTTGACTTTTCACGCCTACCTTTTTCCTTTTGAGATTTGAGTGGTGGTGATAGAGACTAATTCTTGTTGATAACAAAGAGATACAAGCCAAGGGTGTAACGATGCATCTTAGAAAAACTCTCAGGAGGTTACCCAACATAGAATTGCTCCAAGCAAAACACGCTTAACTATAAGTTCTTATGATTGAGCTACCATAAAATAAAGTGCACCTTGTTGGTATAGTTAGTACTTTTAAATTCTTTTAAGTCTTTCTTAGACATCCTATCTTGAGGATTACTCTCATTCGAATGTGATCTCAGTTTATTCATGTATTGTTCCTTTACTCGGGTGTCACAAAGGGAGATTCCGAGAACCCCTATCTCTTCTAAGCCAAAAGCATAACTAACCAAAACCACATATACCTCTAAGCCAAAGCTTTTATTTGTATGCTTCCTCATATTTACTCAAGCTTACATCATAATCGTCACATGGACCCCACTACAACAATCCTCACACACACTTTTATATCATTTTCTGCCCCCTCATCCTCTTTGTATTTTTGACATTAAGCATTGGGGGTCGATCAAGTTGTTCTATCATGTTGACAAGCTTCCGTATGGATAACTATTTCTGCACCTAAACACAAAACTTCTTGTCCCTCAGCGATAAATAGTTGGTTAGTGTAGAATACGCTTGTTTGTTTCTTAAGATATCCCATTGTAATTCTGAAAGGGCTTTATGTTATAAGAGGAATTCAATTGGGGATTCTGTTGATTTGTAACTTCCTAGGAACCACTTGGGAAGATTTCCTACAATGACCATCAATAAGCTTGTGTCTAGGTCCCCAAATCCAAATCATCATGCAAGAATTGATGATTATTCGATTTCAATAATTAAAACACAATACCAGTACCATGATTCTTGCACTATGCGGTTATTCTTATAACATGTCCTTTTAACTATTTGTTAAGTTTGGTCTCTGAATCTTCTGGTCATCTATTATCAATTACTTCTCTATTTCTAGCTCTCTTTTTTTAACCTTCTCTTTAATGTCTCACTTTCAGGCAACATTTTTACTTTGTGTTGCTGGCTACAGTTCAAGTAGTCAAAGTTCTTCTCTAAAGCCCTGTATCACAGGGAACTTTATTCACGATTGGTACATATTCAATTTTGGTTCCTTTCTCTGTATATGTATAATGTCACTGGTTCTCCTTTTGGCTTTTAGTTTTTGAAAGTTTGAAAAAAAGGCATAATTTTTTTGTTTGCTAAATTCTTGAAGTCTGATTTGACCACTTATCTAAGTATCAGTTTTTTTTGCTGCTTAACAGGTGGTTTGGTATGCAGTTGAATCCTCACTTTTTGGGCATCGATCTCAAGTATGTTACTTATTTCGAACTTTATCACGTACCATTCAGGAATAATGTTATTAGTTTAAACGCCACTTCTTATGCAACGACGCTCTCAGCTGTTTACATTTATTGATTTTCAATATTCTTATTACGTTCACTAACAGATGGTCCTTTGCACCTGATTTCATATTGACAAGAATGCTTTAAGTCCTCCTGTAGATTTTTCTTTGTGAGAGCTGGAATGATGGGGTGGCTACTTATTAACCTATCAGTTCTTGCCAGAAGTGTTTTGGACGCGACCTTAAGCCAATCGATGATTCTCTACCAGACATTTTGTGCGGTAAACTGATAGTTTGTATTCCTTCCTTTTTTTTCCTTTTATTTTGGTGGCATCTAATGGTTGGATGTTTGGTCTAATATGTTGATTCAGTTATATGTGCTGGACTACTTCGTTCACGAGGAGTACATGACCTCCACGTAAGTGGATTTTCAGATCATTCTTTCCTGTTGTTTGAAACATATTTTGAAATGCTTCTGAGCTCGGTCTCTTGATTGAATAAGAGCCAAGGATCTTCAACATCAACAAGTCTGCCAATGAAAAGTAATGAATATAAACCCTGACCAATTCCTTGGGCACTAGGGAAATAAAATTGTGAATGGCATCCGTTAAAATGTTATAGCAGAAGCTTATATGTTGCTACTCTAGGATACCGGTGCTCGTTGAATCTCAGTTATGTTATTCTTATTATGACTATTGTCTGTCTGAGGTAGTTGGAGTCTCTGTATTTTATCCATATTGCCGGCTTCTAAAACATTTTTTAGCCTAACTGGTTACTTGGAGACAGTCATGAATCTGGTTCTGCCCTTCATCAACTCTCTAATCAGCAACCGAATTACCTTGTACGAAAAACGTGTCATTTCAAAAGACAAGTTTCTGGTGAATAATAGCTCTGTTTGTATAACATAAAATTGTCTCCATTAGATTTTGTGCCTGCCATTATGAATTCTTAATTGCCATTTTCATATGAACTGTGGTCATTATACATTTTTATGCTTGGAGCACTCGCTTCATTTCACTTGCGATCTTGTGCAATCAGACTGAATATTAAGGCATGATTTATCTTGTGTAGATGGGACATAATTGCTGAAAGATTAGGCTTCATGCTGGTGTTTGGAGATCTTGTTTGGATTCCTTTTACATTTAGCATTCAGGTAAATAATGTTTACCAGTTTCTCTACTGCCACTTAAGCATTCGCTTTCCCTTGATCTTCATTAAACTAGTGAGAAGCTGGAGAGGGGAACAAGACATTTCTTATAAGAGTGTGGAAACCTCTTCCTAACAGACGCGTTTAAAACCTTGGGGGAAACTAAGGAAAAACCCAAAGAGGACAATATCTGCTAGCAGTGGGCTTGAGCTGTTACAAATGGTATCAAAACTAGACACCGGATGATGTGCCAGTTAGGACGTTGGGTCTCCAAGGGGGGTGGATTGTGACATCCCACATCGGTTGGAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTCCCAAATAGACGCGTTTTAAAACTTTGAGGGGAAGCCCAAAAGAGAAAGCCGAAAGAGGACAATATCTGCTAACGGTGGGCTTGGACTGTTACACAAGCGCAAAACCTCCTTTAGGAATATAGAAAGATCACTCTGTACATGAAGTAAACAGAAAAATTATAACCCTTGGTAAAATATAAGAAAACATCCCCACTAGAAAATCATTCACAAATTGTTCTTCTTCCACACTACTTTTTTAAGAAACCTCCTCGAAATACAAAAACCCCATGAAAGCATAACAAAAAAGAATATTAAAGAGCTTAGAAGTTTGAATCCTCAACTCCACATGTTGATCTAACAAAAGAGAATACACGAGCTTCCCACAAAGACCTCCTTCTATCTTTCAATCTAGGAGGCTATTCCACAGACTTTCATCTGCTTATTAAAAGCCAACTCCTTATGGACAAATGAAAGGAATTCAAGATCATCGTCTAGTTCTGCTGCATTATACTTGGCACTTATTAGTCATCTTGTTGACTAAAACTTTTGCATGGAACCAGGATCATTGTCGCACTTGCTCTGATATTGTTTGTATTTTGTGTCCAAACGGATTCTTAATTCTTCAATTTCAAACTGGAACGCACCCATATGAGTTCAGATTGTCTTGCGCATTTCGCCATCACAAATTCTGGACCATTATCTTCATCCTTCAATTTATCTTTTAGAGCTTAATATAGGCTCTATGTTAAACTATCTGTATATTGTAAGTAGTAATGGATCGTAAATTTGTTTTCATATTCAGATTATCTGTGTTCGCTTTTGTTATAGGGTTGGTGGCTTTTAACAAACAAGGCTGAGCTAACAACAGCTGCTACCATAGCTAACTGCTCGGTCTTTTTAATCGGGTAGCTATCACGACGTGACATGACTTATCATTGACATTTTCTTTTTCCTTGTCTTGAAGGTTCACTAAGATGTGAGATTTTTGTGTACTAATTTGGGCAGATATCTTGTGTTTAGAGGATCTAACAAGCAAAAACACATGTTTAAAAAGAACAACAAAGCGCTTATATGGGGTAGGCCTCCCAAAGTTGTAGGAGGAAAGTTGCTTGCTTCTGGTTATTGGTAAGATTTTCATCTATTTTTGTTTATTTGTTGTCATAGGCATAACGTCTCATTAAGTGTTTTGAGGGGAACAGAATTTTTCTGATAGCTTTTTAGGGCTATACTGCAATGTTCTCGTTTCTTGTTTTTCTTTCCGAAGTGTGTTTGATAATGGTAACGTTTCTTGTTCTCTCAATAGGTTAAAGTTTTTCTCGAAAATAATACTTTAGAATAAATTCTTCAAATTTTATACACAATTTTAGAGACTCTTAAAAATGAAAAACAAGAAACGTAAAACGATCAATCCATGAACACGTGTTTCTTAGAAACTAGAAACAATAAACAAGAAACGAGGATATTGCCAAGACCCCTGAATTTTTCGATCTTGAAACTTGAGGCCTGTTGAGATCTTACATTTTGCAAATCATGGGTATTGTTTGGACACCATTTCTCAGGACATGGGAGGCCACCCTATCACTAAAAGGTGGATCAATTTCAATCCACTTTTATTTCCTCATACTCATCTGAAATAACTATGCAATCCCGGTGTCTCGTCACTTTGACCCTCTGTTCACGTCTCTCGTTCTTCACGGCCTAAAAATCTTATACTTGCTGTTGAAACAGGGGAATTGCTAGGCATTGTAATTACCTTGGAGACTTGTTGCTTGCACTTTCTTTCAGCTTACCATGTGGGATAAGGTAATTTATTGGTTCCTCTTTCTTTGTAGCCAAAAGAAAAGAAAGAAGAAGAAATCTGATAATCCCGTCATTTTTGCAGCTCCCCAATTCCATACTTTTATCCCACATATCTCCTCCTTCTGCTAATATGGAGAGAAAGACGGGATGAAGCTCGATGCGCTGAGAAATATAAAGACATTTGGGCGGAGTATACCAAACTCGTCCCTTGGAGAATACTGCCTTATGTTTATTAG

mRNA sequence

AGATTTACAAGTTAGCAGAGCAGTTGAACCAGCGCCCGTGGATTTAGTAGCTGCAATGAACAGACTCGGGGCTCGAGCTAAAGGCAGCCATAGTTGAAATTAGACAATCCCGAGAAGAAATTAGTTCCAATTTTGCAGCAAATTATCATTGTTACCAGAGGAAACAAGTCGATCGAGCGACAAAATCCGAACAAGTTGTCAATGATCGATTTCTTGAGGAATATGGATCTGGAGTTTCTTCTTCAACTTGCAATCCCTTCTTGGGAAGCTGTTGCTATACTTTCCATTTTCTTCTTTTACTTGGCGATTGCTGGCTCTGTTCTACCTGGAAGGATTATCCCAGGAGTGATCTTGTCGGACCGCACTAGGCTTCATTATCGCTGCAATGGTCTACTCTCTCTTCTGGTGCTTGTTGCACTTCTTGGGATTGGTGTTAAGATGAATTACATTTCGCCCACTGCAATTTCAGATAGAGGGGTGGAGCTGTTATCCGCTACCTTCTGGTTTAGTGTCCTGGCAACATTTTTACTTTGTGTTGCTGGCTACAGTTCAAGTAGTCAAAGTTCTTCTCTAAAGCCCTGTATCACAGGGAACTTTATTCACGATTGGTGGTTTGGTATGCAGTTGAATCCTCACTTTTTGGGCATCGATCTCAAATTTTTCTTTGTGAGAGCTGGAATGATGGGGTGGCTACTTATTAACCTATCAGTTCTTGCCAGAAGTGTTTTGGACGCGACCTTAAGCCAATCGATGATTCTCTACCAGACATTTTGTGCGTTATATGTGCTGGACTACTTCGTTCACGAGGAGTACATGACCTCCACATGGGACATAATTGCTGAAAGATTAGGCTTCATGCTGGTGTTTGGAGATCTTGTTTGGATTCCTTTTACATTTAGCATTCAGGGTTGGTGGCTTTTAACAAACAAGGCTGAGCTAACAACAGCTGCTACCATAGCTAACTGCTCGGTCTTTTTAATCGGATATCTTGTGTTTAGAGGATCTAACAAGCAAAAACACATGTTTAAAAAGAACAACAAAGCGCTTATATGGGGTAGGCCTCCCAAAGTTGTAGGAGGAAAGTTGCTTGCTTCTGGTTATTGGGGAATTGCTAGGCATTGTAATTACCTTGGAGACTTGTTGCTTGCACTTTCTTTCAGCTTACCATGTGGGATAAGCTCCCCAATTCCATACTTTTATCCCACATATCTCCTCCTTCTGCTAATATGGAGAGAAAGACGGGATGAAGCTCGATGCGCTGAGAAATATAAAGACATTTGGGCGGAGTATACCAAACTCGTCCCTTGGAGAATACTGCCTTATGTTTATTAG

Coding sequence (CDS)

ATGATCGATTTCTTGAGGAATATGGATCTGGAGTTTCTTCTTCAACTTGCAATCCCTTCTTGGGAAGCTGTTGCTATACTTTCCATTTTCTTCTTTTACTTGGCGATTGCTGGCTCTGTTCTACCTGGAAGGATTATCCCAGGAGTGATCTTGTCGGACCGCACTAGGCTTCATTATCGCTGCAATGGTCTACTCTCTCTTCTGGTGCTTGTTGCACTTCTTGGGATTGGTGTTAAGATGAATTACATTTCGCCCACTGCAATTTCAGATAGAGGGGTGGAGCTGTTATCCGCTACCTTCTGGTTTAGTGTCCTGGCAACATTTTTACTTTGTGTTGCTGGCTACAGTTCAAGTAGTCAAAGTTCTTCTCTAAAGCCCTGTATCACAGGGAACTTTATTCACGATTGGTGGTTTGGTATGCAGTTGAATCCTCACTTTTTGGGCATCGATCTCAAATTTTTCTTTGTGAGAGCTGGAATGATGGGGTGGCTACTTATTAACCTATCAGTTCTTGCCAGAAGTGTTTTGGACGCGACCTTAAGCCAATCGATGATTCTCTACCAGACATTTTGTGCGTTATATGTGCTGGACTACTTCGTTCACGAGGAGTACATGACCTCCACATGGGACATAATTGCTGAAAGATTAGGCTTCATGCTGGTGTTTGGAGATCTTGTTTGGATTCCTTTTACATTTAGCATTCAGGGTTGGTGGCTTTTAACAAACAAGGCTGAGCTAACAACAGCTGCTACCATAGCTAACTGCTCGGTCTTTTTAATCGGATATCTTGTGTTTAGAGGATCTAACAAGCAAAAACACATGTTTAAAAAGAACAACAAAGCGCTTATATGGGGTAGGCCTCCCAAAGTTGTAGGAGGAAAGTTGCTTGCTTCTGGTTATTGGGGAATTGCTAGGCATTGTAATTACCTTGGAGACTTGTTGCTTGCACTTTCTTTCAGCTTACCATGTGGGATAAGCTCCCCAATTCCATACTTTTATCCCACATATCTCCTCCTTCTGCTAATATGGAGAGAAAGACGGGATGAAGCTCGATGCGCTGAGAAATATAAAGACATTTGGGCGGAGTATACCAAACTCGTCCCTTGGAGAATACTGCCTTATGTTTATTAG

Protein sequence

MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY

Homology

[Close]

BLAST of Carg00929 vs. NCBI nr
Match: XP_022947797.1 (delta(14)-sterol reductase-like [Cucurbita moschata] >KAG6596538.1 Delta(14)-sterol reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028071.1 Delta(14)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 761.5 bits (1965), Expect = 3.3e-216
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0


Query: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60
           MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR
Sbjct: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60

Query: 61  CNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120
           CNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ
Sbjct: 61  CNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120

Query: 121 SSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180
           SSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL
Sbjct: 121 SSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180

Query: 181 SQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLL 240
           SQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLL
Sbjct: 181 SQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLL 240

Query: 241 TNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGY 300
           TNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGY
Sbjct: 241 TNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGY 300

Query: 301 WGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIW 360
           WGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIW
Sbjct: 301 WGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIW 360

Query: 361 AEYTKLVPWRILPYVY 377
           AEYTKLVPWRILPYVY
Sbjct: 361 AEYTKLVPWRILPYVY 376

[Close]

BLAST of Carg00929 vs. NCBI nr
Match: XP_023540573.1 (delta(14)-sterol reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 758.8 bits (1958), Expect = 2.2e-215
Identity = 374/376 (99.47%), Postives = 376/376 (100.00%), Query Frame = 0


Query: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60
           MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR
Sbjct: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60

Query: 61  CNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120
           CNGLLSLLVL+ALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ
Sbjct: 61  CNGLLSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120

Query: 121 SSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180
           SSSLKPCITGNFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL
Sbjct: 121 SSSLKPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180

Query: 181 SQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLL 240
           SQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLL
Sbjct: 181 SQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLL 240

Query: 241 TNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGY 300
           TNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGY
Sbjct: 241 TNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGY 300

Query: 301 WGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIW 360
           WGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIW
Sbjct: 301 WGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIW 360

Query: 361 AEYTKLVPWRILPYVY 377
           AEYTKLVPWRILPYVY
Sbjct: 361 AEYTKLVPWRILPYVY 376

[Close]

BLAST of Carg00929 vs. NCBI nr
Match: XP_023005820.1 (delta(14)-sterol reductase-like [Cucurbita maxima])

HSP 1 Score: 737.6 bits (1903), Expect = 5.2e-209
Identity = 364/369 (98.64%), Postives = 366/369 (99.19%), Query Frame = 0


Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDLEFLLQLAIPSWEAVAIL IFF YLAIAGSVLPGRIIPGVIL DRTRLHYRCNGLLSL
Sbjct: 1   MDLEFLLQLAIPSWEAVAILFIFFVYLAIAGSVLPGRIIPGVILPDRTRLHYRCNGLLSL 60

Query: 68  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAG+SSSSQSSSLKPC
Sbjct: 61  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGHSSSSQSSSLKPC 120

Query: 128 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 247
           QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNK+ELT
Sbjct: 181 QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKSELT 240

Query: 248 TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC 307
           TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC 300

Query: 308 NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV 367
           NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 368 PWRILPYVY 377
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Carg00929 vs. NCBI nr
Match: KAA0043924.1 (delta(14)-sterol reductase [Cucumis melo var. makuwa] >TYK25215.1 delta(14)-sterol reductase [Cucumis melo var. makuwa])

HSP 1 Score: 716.1 bits (1847), Expect = 1.6e-202
Identity = 350/369 (94.85%), Postives = 361/369 (97.83%), Query Frame = 0


Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL FL Q +IPSWEAVAIL IFFFYLAIAGSVLPGR+IPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 68  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVLVALLG+GVKMNYISPTAISDRG+ELLSATFWFSVLATFLLCVAGYSSSSQS+SLKP 
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 128 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVL+ATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 247
           QTFCA+YVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT
Sbjct: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240

Query: 248 TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC 307
           TAATIANC +FLIGYLVFRGSNKQKHMFKKNNKALIWG+PPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLIFLIGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 308 NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV 367
           NYLGDLLLALSFSLPCGISSPIPYFYP YLL+LLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 368 PWRILPYVY 377
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Carg00929 vs. NCBI nr
Match: XP_038904165.1 (delta(14)-sterol reductase isoform X1 [Benincasa hispida])

HSP 1 Score: 714.9 bits (1844), Expect = 3.6e-202
Identity = 345/369 (93.50%), Postives = 362/369 (98.10%), Query Frame = 0


Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL FLLQ +IPSWEAVAIL IFFFYLAIAGSVLPGR+IPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 68  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVL+ALLG+GVKMNYISPTAISDRG+ELLSATFWFSVLATFLLCVAGYSSSSQSSSLKP 
Sbjct: 61  LVLIALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPY 120

Query: 128 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           +TGNF+HDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLS+LARS+L+ATLSQSMILY
Sbjct: 121 VTGNFVHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSILARSILNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 247
           QTFCA+YVLDYF+HEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT
Sbjct: 181 QTFCAIYVLDYFIHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240

Query: 248 TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC 307
           TAATIANC VF+IGYLVFRGSNKQKHMFKKNNKALIWG+PPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLVFVIGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 308 NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV 367
           NYLGDLLLALSFSLPCGISSPIPYFYP YLL+LLIWRERRD+ARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDDARCAEKYKDIWAEYTKLV 360

Query: 368 PWRILPYVY 377
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Carg00929 vs. ExPASy Swiss-Prot
Match: Q9LDR4 (Delta(14)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=FK PE=1 SV=2)

HSP 1 Score: 579.3 bits (1492), Expect = 3.1e-164
Identity = 276/372 (74.19%), Postives = 319/372 (85.75%), Query Frame = 0


Query: 5   LRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGL 64
           L +MDL  LL    PS ++V +L  +F YLA+AG +LPG++I GV+LSD ++L YRCNGL
Sbjct: 2   LLDMDLGVLL----PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGL 61

Query: 65  LSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSL 124
           L+L++LVA+LGI  K+  +SP  ++DRG+ELLSATF F VL T  L V G SSS++ SSL
Sbjct: 62  LALILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSL 121

Query: 125 KPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSM 184
           KP ++GN +HDWWFG+QLNP F+ IDLKFFFVRAGMMGWLLINLS+LA+SV D +LSQSM
Sbjct: 122 KPHVSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSM 181

Query: 185 ILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKA 244
           ILYQ FCALY+LDYFVHEEYMTSTWDIIAERLGFMLVFGDL+WIPFTFSIQGWWLL NK 
Sbjct: 182 ILYQIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQGWWLLHNKV 241

Query: 245 ELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIA 304
           ELT  A + NC VFLIGY+VFRG+NKQKH+FKKN K  IWG+PP VVGGKLL SGYWGIA
Sbjct: 242 ELTVPAIVVNCLVFLIGYMVFRGANKQKHIFKKNPKTPIWGKPPVVVGGKLLVSGYWGIA 301

Query: 305 RHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYT 364
           RHCNYLGDL+LALSFSLPCGISSP+PYFYP YLL+LLIWRERRDE RCAEKYK+IWAEY 
Sbjct: 302 RHCNYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEKYKEIWAEYL 361

Query: 365 KLVPWRILPYVY 377
           +LVPWRILPYVY
Sbjct: 362 RLVPWRILPYVY 369

[Close]

BLAST of Carg00929 vs. ExPASy Swiss-Prot
Match: G4SW86 (Delta(14)-sterol reductase OS=Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z) OX=1091494 GN=erg PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 5.2e-71
Identity = 148/371 (39.89%), Postives = 221/371 (59.57%), Query Frame = 0


Query: 12  FLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLVLV 71
           F   +A P+W A  + + +F   A      PG  + G+ L D +RL YR NG+ S L  +
Sbjct: 62  FWSHVAPPTWHAAGLYAAWFLGQAALQVWAPGPTVQGMKLPDGSRLDYRMNGIFSFLFTL 121

Query: 72  ALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGN 131
           A++   V M ++  T + D+   LL+    F+ +    L   G +        +P  TG 
Sbjct: 122 AVVFGLVTMGWLDATVLYDQLGPLLTVVNIFTFVFAGFLYFWGLNGKQWE---RP--TGR 181

Query: 132 FIHDWWFGMQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSV-LDATLSQSMILYQT 191
             +D++ G  LNP    +DLK F   R GM+ WLL+NLS+ A+   L  T++  M+L   
Sbjct: 182 PFYDYFMGTALNPRIGSLDLKLFCEARPGMIFWLLMNLSMAAKQYELHGTVTVPMLLVVG 241

Query: 192 FCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTA 251
           F + Y++DYF+HEE + +TWDI  E+ G+ML +GDLVW+PFT+++Q  +L+ +  +L   
Sbjct: 242 FQSFYLIDYFIHEEAVLTTWDIKHEKFGWMLCWGDLVWLPFTYTLQAQYLVHHTHDLPVW 301

Query: 252 ATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVV----GGKLLASGYWGIAR 311
             IA  ++ L GY +FRG+N QKH F+++   ++WG+P K +    G  LL SG+WGIAR
Sbjct: 302 GIIAIVALNLAGYAIFRGANIQKHHFRRDPNRIVWGKPAKYIKTKQGSLLLTSGWWGIAR 361

Query: 312 HCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTK 371
           H NY GDL++ALS+ LP    SPIPYF+  Y  +LL+ RE+RD+A C  KY + W +Y K
Sbjct: 362 HMNYFGDLMIALSWCLPAAFGSPIPYFHIVYFTILLLHREKRDDAMCLAKYGEDWLQYRK 421

Query: 372 LVPWRILPYVY 377
            VPWRI+P +Y
Sbjct: 422 KVPWRIVPKIY 427

[Close]

BLAST of Carg00929 vs. ExPASy Swiss-Prot
Match: Q71KT5 (Delta(14)-sterol reductase TM7SF2 OS=Mus musculus OX=10090 GN=Tm7sf2 PE=1 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 8.1e-64
Identity = 148/366 (40.44%), Postives = 222/366 (60.66%), Query Frame = 0


Query: 21  WEAVAILSIFFFY-LAIAGSVLPGR-IIPGVILSDRTRLHYRCNGLLSLLVLVALLGIGV 80
           W   A+L +F +  L +A  +LP R +  G+ L D++RL Y  NG  +L++   L+G+GV
Sbjct: 58  WSPWALLLLFIWLGLQVALYLLPARKVAEGLELKDKSRLRYPINGFQALVLTALLMGLGV 117

Query: 81  KMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSS--SQSSSLKP-CITGNFIHD 140
            +  +   A+    + L  AT     L +F+  +  Y+ +  + +S+L P   +GN ++D
Sbjct: 118 SVG-LPLGALPGMLLPLAFAT----TLTSFIFSLLLYAKALVAPASALAPGGNSGNSMYD 177

Query: 141 WWFGMQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSV-LDATLSQSMILYQTFCAL 200
           ++ G +LNP     D K+F  +R G++GW+ INL++L +   L  + S +M L   F  L
Sbjct: 178 FFLGRELNPRLGSFDFKYFCELRPGLIGWVFINLALLMQEAELRGSPSLAMWLVNGFQLL 237

Query: 201 YVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIA 260
           YV D   +EE + +T DII +  GFMLVFGDL W+PFT+S+Q  +LL +   L     + 
Sbjct: 238 YVGDALWYEESVLTTMDIIHDGFGFMLVFGDLAWVPFTYSLQAQFLLYHPQPLGLPMALL 297

Query: 261 NCSVFLIGYLVFRGSNKQKHMFKKN-NKALIWGRP--PKVVGGKLLASGYWGIARHCNYL 320
            C + +IGY +FRG+N QK+ F+KN +   + G    P   G +LL SG+WG+ RH NYL
Sbjct: 298 ICLLKVIGYYIFRGANSQKNTFRKNPSDPSVAGLETIPTATGRQLLVSGWWGMVRHPNYL 357

Query: 321 GDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWR 377
           GDL++AL++SLPCG+S  +PYFY  Y   LL+ RE RDE +C +KY   W EY K VP+R
Sbjct: 358 GDLIMALAWSLPCGLSHLLPYFYVLYFTALLVHREARDEQQCLQKYGRAWQEYCKRVPYR 417

[Close]

BLAST of Carg00929 vs. ExPASy Swiss-Prot
Match: O08984 (Delta(14)-sterol reductase LBR OS=Rattus norvegicus OX=10116 GN=Lbr PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.1e-60
Identity = 143/365 (39.18%), Postives = 209/365 (57.26%), Query Frame = 0


Query: 21  WEA-VAILSIFFFYLAIAGSVLP-GRIIPGVILSDRTRLHYRCNGLLSLLVLVALLGIGV 80
           WEA V  + + +F+L    S+LP G+++ G  L D  RL YR NGL + ++  A +G  V
Sbjct: 259 WEARVCGVYLLWFFLQALFSLLPVGKVVEGTPLVDGRRLKYRLNGLYAFILTSAAVGTAV 318

Query: 81  KMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIHDWWF 140
             + I    +    ++   A   FSV+ +  L     S       L P  +GN ++D++ 
Sbjct: 319 FWD-IELYYLYTHFLQFALAAIVFSVVLSVYLYAR--SLKVPRDELSPASSGNAVYDFFI 378

Query: 141 GMQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSVL---DATLSQSMILYQTFCALY 200
           G +LNP     DLKFF  +R G++GW++INL +L   +     +  S +M L  +F  LY
Sbjct: 379 GRELNPRIGAFDLKFFCELRPGLIGWVVINLVMLLAEMKVQERSAPSLAMTLVNSFQLLY 438

Query: 201 VLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIAN 260
           V+D    EE + +T DII +  GFML FGDLVW+PFT+S+Q ++L+ +  +L+   T   
Sbjct: 439 VVDALWFEEALLTTMDIIHDGFGFMLAFGDLVWVPFTYSLQAFYLVNHPQDLSWPLTSVI 498

Query: 261 CSVFLIGYLVFRGSNKQKHMFKKN---NKALIWGRPPKVVGGKLLASGYWGIARHCNYLG 320
            ++ L GY++FR +N QK+ F+KN    K       P      LL SG+WG  RH NYLG
Sbjct: 499 IALKLCGYVIFRCANSQKNAFRKNPTDPKLAHLKTIPTSTWKSLLVSGWWGFVRHPNYLG 558

Query: 321 DLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRI 377
           DL++AL++SLPCG +  +PYFY  Y   LLI RE RDE +C  KY   W +Y + VP+RI
Sbjct: 559 DLIMALAWSLPCGFNHILPYFYVIYFTALLIHREARDEHQCRRKYGLAWEKYCQRVPYRI 618

[Close]

BLAST of Carg00929 vs. ExPASy Swiss-Prot
Match: Q3U9G9 (Delta(14)-sterol reductase LBR OS=Mus musculus OX=10090 GN=Lbr PE=1 SV=2)

HSP 1 Score: 233.8 bits (595), Expect = 3.2e-60
Identity = 144/375 (38.40%), Postives = 213/375 (56.80%), Query Frame = 0


Query: 21  WE--AVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLVLVALLGI-- 80
           WE     +  ++FF  A+   +  G++  G  L D  RL YR NGL + ++  A LG   
Sbjct: 265 WEPRVCGVYLLWFFVQALFHLLPVGKVAEGTPLVDGRRLQYRLNGLYAFILTSAALGAAV 324

Query: 81  --GVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIH 140
             GV++ Y     +    ++L  A   FSVL +  L V   S  +    L P  +GN ++
Sbjct: 325 FWGVELCY-----LYTHFLQLALAATGFSVLLSAYLYVR--SLRAPREELSPASSGNAVY 384

Query: 141 DWWFGMQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSVL---DATLSQSMILYQTF 200
           D++ G +LNP     DLKFF  +R G++GW++INL +L   +     A  S +MIL  +F
Sbjct: 385 DFFIGRELNPRLGAFDLKFFCELRPGLIGWVVINLVMLLMEMKIQERAAPSLAMILVNSF 444

Query: 201 CALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAA 260
             LYV+D   +EE + ++ DI+ +  GFML FGDLVW+PFT+S+Q ++L+++  +L+   
Sbjct: 445 QLLYVVDALWNEEALLTSMDIMHDGFGFMLAFGDLVWVPFTYSLQAFYLVSHPHDLSWPL 504

Query: 261 TIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKV---------VGGKLLASGYW 320
                ++ L GY++FR +N QK+ F+KN         PK+          G  LL SG+W
Sbjct: 505 ASVIIALKLCGYVIFRCANSQKNAFRKN------PTDPKLAHLKTIHTSTGKSLLVSGWW 564

Query: 321 GIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWA 377
           G  RH NYLGDL++AL++SLPCG +  +PYFY  Y   LLI RE RDE +C  KY   W 
Sbjct: 565 GFVRHPNYLGDLIMALAWSLPCGFNHLLPYFYIIYFTALLIHREARDEHQCRRKYGLAWE 624

[Close]

BLAST of Carg00929 vs. ExPASy TrEMBL
Match: A0A6J1G7F4 (delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111451551 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.6e-216
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0


Query: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60
           MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR
Sbjct: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60

Query: 61  CNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120
           CNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ
Sbjct: 61  CNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120

Query: 121 SSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180
           SSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL
Sbjct: 121 SSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180

Query: 181 SQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLL 240
           SQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLL
Sbjct: 181 SQSMILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLL 240

Query: 241 TNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGY 300
           TNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGY
Sbjct: 241 TNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGY 300

Query: 301 WGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIW 360
           WGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIW
Sbjct: 301 WGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIW 360

Query: 361 AEYTKLVPWRILPYVY 377
           AEYTKLVPWRILPYVY
Sbjct: 361 AEYTKLVPWRILPYVY 376

[Close]

BLAST of Carg00929 vs. ExPASy TrEMBL
Match: A0A6J1KYF4 (delta(14)-sterol reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111498711 PE=3 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 2.5e-209
Identity = 364/369 (98.64%), Postives = 366/369 (99.19%), Query Frame = 0


Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDLEFLLQLAIPSWEAVAIL IFF YLAIAGSVLPGRIIPGVIL DRTRLHYRCNGLLSL
Sbjct: 1   MDLEFLLQLAIPSWEAVAILFIFFVYLAIAGSVLPGRIIPGVILPDRTRLHYRCNGLLSL 60

Query: 68  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAG+SSSSQSSSLKPC
Sbjct: 61  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGHSSSSQSSSLKPC 120

Query: 128 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 247
           QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNK+ELT
Sbjct: 181 QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKSELT 240

Query: 248 TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC 307
           TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC 300

Query: 308 NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV 367
           NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 368 PWRILPYVY 377
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Carg00929 vs. ExPASy TrEMBL
Match: A0A5A7TKR7 (Delta(14)-sterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003450 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 7.8e-203
Identity = 350/369 (94.85%), Postives = 361/369 (97.83%), Query Frame = 0


Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL FL Q +IPSWEAVAIL IFFFYLAIAGSVLPGR+IPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 68  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVLVALLG+GVKMNYISPTAISDRG+ELLSATFWFSVLATFLLCVAGYSSSSQS+SLKP 
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 128 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVL+ATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 247
           QTFCA+YVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT
Sbjct: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240

Query: 248 TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC 307
           TAATIANC +FLIGYLVFRGSNKQKHMFKKNNKALIWG+PPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLIFLIGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 308 NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV 367
           NYLGDLLLALSFSLPCGISSPIPYFYP YLL+LLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 368 PWRILPYVY 377
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Carg00929 vs. ExPASy TrEMBL
Match: A0A6J1F6L0 (delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111441342 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 3.0e-202
Identity = 349/369 (94.58%), Postives = 362/369 (98.10%), Query Frame = 0


Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL+ LLQ+ IPSWEAVAIL IFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL
Sbjct: 1   MDLDSLLQVLIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 60

Query: 68  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           L+LVALLGIGVKMNYISPTAISDRG+ELLSATFWFSVLATFLLC+AGYSSSSQSSSLKP 
Sbjct: 61  LLLVALLGIGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCIAGYSSSSQSSSLKPR 120

Query: 128 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
            TGNFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVL+ATLSQSMILY
Sbjct: 121 ATGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 247
           QTFCA+YV+DYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTN+AELT
Sbjct: 181 QTFCAIYVIDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNRAELT 240

Query: 248 TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC 307
           TAATIANC VFLIGYLVFRGSNKQKHMFKKN+KALIWG+PPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNSKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 308 NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV 367
           NYLGDLLLALSFSLPCGISSPIPYFYP YLL+LLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 368 PWRILPYVY 377
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Carg00929 vs. ExPASy TrEMBL
Match: A0A1S3B6J9 (LOW QUALITY PROTEIN: delta(14)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC103486569 PE=3 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 5.1e-202
Identity = 348/369 (94.31%), Postives = 360/369 (97.56%), Query Frame = 0


Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL FL Q +IPSWEAVAIL IFFFYLAIAGSVLPGR+IPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 68  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVLVALLG+GVKMNYISPTAISDRG+ELLSATFWFSVLATFLLCVAGYSSSSQS+SLKP 
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 128 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVL+ATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 247
           QTFCA+YVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT
Sbjct: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240

Query: 248 TAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHC 307
           TAATIANC +F +GYLVFRGSNKQKHMFKKNNKALIWG+PPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLIFXLGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 308 NYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLV 367
           NYLGDLLLALSFSLPCGISSPIPYFYP YLL+LLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 368 PWRILPYVY 377
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Carg00929 vs. TAIR 10
Match: AT3G52940.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 579.3 bits (1492), Expect = 2.2e-165
Identity = 276/372 (74.19%), Postives = 319/372 (85.75%), Query Frame = 0


Query: 5   LRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGL 64
           L +MDL  LL    PS ++V +L  +F YLA+AG +LPG++I GV+LSD ++L YRCNGL
Sbjct: 2   LLDMDLGVLL----PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGL 61

Query: 65  LSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSL 124
           L+L++LVA+LGI  K+  +SP  ++DRG+ELLSATF F VL T  L V G SSS++ SSL
Sbjct: 62  LALILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSL 121

Query: 125 KPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSM 184
           KP ++GN +HDWWFG+QLNP F+ IDLKFFFVRAGMMGWLLINLS+LA+SV D +LSQSM
Sbjct: 122 KPHVSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSM 181

Query: 185 ILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKA 244
           ILYQ FCALY+LDYFVHEEYMTSTWDIIAERLGFMLVFGDL+WIPFTFSIQGWWLL NK 
Sbjct: 182 ILYQIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQGWWLLHNKV 241

Query: 245 ELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIA 304
           ELT  A + NC VFLIGY+VFRG+NKQKH+FKKN K  IWG+PP VVGGKLL SGYWGIA
Sbjct: 242 ELTVPAIVVNCLVFLIGYMVFRGANKQKHIFKKNPKTPIWGKPPVVVGGKLLVSGYWGIA 301

Query: 305 RHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYT 364
           RHCNYLGDL+LALSFSLPCGISSP+PYFYP YLL+LLIWRERRDE RCAEKYK+IWAEY 
Sbjct: 302 RHCNYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEKYKEIWAEYL 361

Query: 365 KLVPWRILPYVY 377
           +LVPWRILPYVY
Sbjct: 362 RLVPWRILPYVY 369

[Close]

BLAST of Carg00929 vs. TAIR 10
Match: AT3G52940.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 327.0 bits (837), Expect = 2.0e-89
Identity = 165/252 (65.48%), Postives = 202/252 (80.16%), Query Frame = 0


Query: 5   LRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGL 64
           L +MDL  LL    PS ++V +L  +F YLA+AG +LPG++I GV+LSD ++L YRCNGL
Sbjct: 2   LLDMDLGVLL----PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGL 61

Query: 65  LSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSL 124
           L+L++LVA+LGI  K+  +SP  ++DRG+ELLSATF F VL T  L V G SSS++ SSL
Sbjct: 62  LALILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSL 121

Query: 125 KPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSM 184
           KP ++GN +HDWWFG+QLNP F+ IDLKFFFVRAGMMGWLLINLS+LA+SV D +LSQSM
Sbjct: 122 KPHVSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSM 181

Query: 185 ILYQTFCALYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKA 244
           ILYQ FCALY+LDYFVHEEYMTSTWDIIAERLGFMLVFGDL+WIPFTFSIQ W  L   A
Sbjct: 182 ILYQIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQAW--LVAFA 241

Query: 245 ELTTAATIANCS 257
           + +   +  +CS
Sbjct: 242 QQSRTNSSCDCS 247

[Close]

BLAST of Carg00929 vs. TAIR 10
Match: AT1G50430.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 165.2 bits (417), Expect = 9.9e-41
Identity = 115/374 (30.75%), Postives = 190/374 (50.80%), Query Frame = 0


Query: 19  PSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLVLVALLGIGV 78
           P+  A  I+  +  + AI   +LPG+ + G I     R  Y+ NGL +  V +A   +G+
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATY-LGL 121

Query: 79  KMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIHDWWF 138
                +P  + D   E+ SA  + S +   LL + G+ + S S S      GN I D+++
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGS---CGNLIIDFYW 181

Query: 139 GMQLNPHF-LGIDLKFFF-VRAGMMGWLLINLSVLARSV-LDATLSQSMILYQTFCALYV 198
           GM+L P      D+K F   R GMM W ++ ++   +   ++  +S SM++      +YV
Sbjct: 182 GMELYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYV 241

Query: 199 LDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANC 258
             +F  E    +T DI  +R GF + +G LVW+P  ++  G +L+ +  EL T   I   
Sbjct: 242 TKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYIL 301

Query: 259 SVFLIGYLVFRGSNKQKHMFKK-NNKALIWGR-PPKVVG-----------GKLLASGYWG 318
              ++   +    ++Q+  F++ N K L+WGR P K+V              LL SG+WG
Sbjct: 302 VAGILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWG 361

Query: 319 IARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAE 377
           +ARH +Y+ ++L A  +++P    + + YFY  +L LLL  R +RD+ RC  KY   W  
Sbjct: 362 LARHFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKL 421

[Close]

BLAST of Carg00929 vs. TAIR 10
Match: AT1G50430.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-39
Identity = 116/375 (30.93%), Postives = 189/375 (50.40%), Query Frame = 0


Query: 19  PSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLV-LVALLGIG 78
           P+  A  I+  +  + AI   +LPG+ + G I     R  Y+ NGL +  V L   LG+ 
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 79  VKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIHDWW 138
                 +P  + D   E+ SA  + S +   LL + G+ + S S S      GN I D++
Sbjct: 122 -WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGS---CGNLIIDFY 181

Query: 139 FGMQLNPHF-LGIDLKFFF-VRAGMMGWLLINLSVLARSV-LDATLSQSMILYQTFCALY 198
           +GM+L P      D+K F   R GMM W ++ ++   +   ++  +S SM++      +Y
Sbjct: 182 WGMELYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVY 241

Query: 199 VLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIAN 258
           V  +F  E    +T DI  +R GF + +G LVW+P  ++  G +L+ +  EL T   I  
Sbjct: 242 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYI 301

Query: 259 CSVFLIGYLVFRGSNKQKHMFKK-NNKALIWGR-PPKVVG-----------GKLLASGYW 318
               ++   +    ++Q+  F++ N K L+WGR P K+V              LL SG+W
Sbjct: 302 LVAGILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWW 361

Query: 319 GIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWA 377
           G+ARH +Y+ ++L A  +++P    + + YFY  +L LLL  R +RD+ RC  KY   W 
Sbjct: 362 GLARHFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWK 421

The following BLAST results are available for this feature:

BLAST of Carg00929 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022947797.1	3.3e-216	100.00	delta(14)-sterol reductase-like [Cucurbita moschata] >KAG6596538.1 Delta(14)-ste...	[more]
XP_023540573.1	2.2e-215	99.47	delta(14)-sterol reductase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023005820.1	5.2e-209	98.64	delta(14)-sterol reductase-like [Cucurbita maxima]	[more]
KAA0043924.1	1.6e-202	94.85	delta(14)-sterol reductase [Cucumis melo var. makuwa] >TYK25215.1 delta(14)-ster...	[more]
XP_038904165.1	3.6e-202	93.50	delta(14)-sterol reductase isoform X1 [Benincasa hispida]	[more]

BLAST of Carg00929 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDR4	3.1e-164	74.19	Delta(14)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=FK PE=1 SV=2	[more]
G4SW86	5.2e-71	39.89	Delta(14)-sterol reductase OS=Methylomicrobium alcaliphilum (strain DSM 19304 / ...	[more]
Q71KT5	8.1e-64	40.44	Delta(14)-sterol reductase TM7SF2 OS=Mus musculus OX=10090 GN=Tm7sf2 PE=1 SV=2	[more]
O08984	1.1e-60	39.18	Delta(14)-sterol reductase LBR OS=Rattus norvegicus OX=10116 GN=Lbr PE=1 SV=1	[more]
Q3U9G9	3.2e-60	38.40	Delta(14)-sterol reductase LBR OS=Mus musculus OX=10090 GN=Lbr PE=1 SV=2	[more]

BLAST of Carg00929 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G7F4	1.6e-216	100.00	delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111451551 PE...	[more]
A0A6J1KYF4	2.5e-209	98.64	delta(14)-sterol reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111498711 PE=3...	[more]
A0A5A7TKR7	7.8e-203	94.85	Delta(14)-sterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A6J1F6L0	3.0e-202	94.58	delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111441342 PE...	[more]
A0A1S3B6J9	5.1e-202	94.31	LOW QUALITY PROTEIN: delta(14)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Carg00929 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52940.1	2.2e-165	74.19	Ergosterol biosynthesis ERG4/ERG24 family	[more]
AT3G52940.2	2.0e-89	65.48	Ergosterol biosynthesis ERG4/ERG24 family	[more]
AT1G50430.2	9.9e-41	30.75	Ergosterol biosynthesis ERG4/ERG24 family	[more]
AT1G50430.1	1.4e-39	30.93	Ergosterol biosynthesis ERG4/ERG24 family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 177..375 e-value: 6.1E-21 score: 77.0
None	No IPR available	PANTHER	PTHR21257	DELTA(14)-STEROL REDUCTASE	coord: 7..376
None	No IPR available	PANTHER	PTHR21257:SF51	BNACNNG50210D PROTEIN	coord: 7..376
IPR001171	Ergosterol biosynthesis ERG4/ERG24	PFAM	PF01222	ERG4_ERG24	coord: 13..376 e-value: 5.7E-89 score: 298.8
IPR018083	Sterol reductase, conserved site	PROSITE	PS01018	STEROL_REDUCT_2	coord: 340..363

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00929-RA	Carg00929-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016126	sterol biosynthetic process
cellular_component	GO:0030176	integral component of endoplasmic reticulum membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0050613	delta14-sterol reductase activity
molecular_function	GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor