Carg00759 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00759
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cysteine desulfurase
Location	Carg_Chr03: 6585011 .. 6586423 (+)
RNA-Seq Expression	Carg00759
Synteny	Carg00759

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCTTCCGCCGTCTCCCGCTTCCGGCCTCTCTCCAATGTCGGAACCCCATGAGGATGAAACTGGGATTTCTATGAAAGGTGTCAAAATCTCTGGACGACCGCTTTATCTTGATATGCAAGCTACATCCCCTGCTGACCCTCGGGTGCTCGATGCTAGGCTTCCTTATTATCTCTCTCGATGTGGTAATCCACCACTATCGGACGCATCTCTATGGTTGGGAATCGGATCATGCCGTCGAGTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTTACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGGCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCACTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGCAGGTGGGTTTTGATATTACCTATTTACCGGTTGGGTCCGATGGTATTGTTGATTTGGAGAAGCTCCGTTCTGTGATTAGACCCGACACTGGCCTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTCATCCAGCCGTTAGAGGAAATTGGGAAAATTTGCAAGGAATTCAATGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGGAGATTCCAATTGATTTGGAGAAGTGGAATGTGAGCCTGAAGAGTATTGGGGCTCTGAATCTAACAAATGCATTCTTAAGAAGGTTAACGATTTTATTTAAGTTCTTAATCTTTGTCTCTTTACTTTTTGTTCTACCTGATTCATCAAAGGACAATTTTTTAGCTAAATGCCTTTTATTTTCTTCTCCCTTCTTTTTGGTTGAATAGGTATGAATTTATGGTTTCTCTTCCTTTTTTACTGTTATATAGCAGATTACATTTCATTCACAAGTTTTTTTTTGTAAGGCGCACAACTTTTTATTAAAGACGATGAGACATCCTCTCGTCTCACTCCCAGCTGACTAAGATTACAGAAATTTCTTAATCAAGAATACAAGAATGGTTATGAAATCATCCTTTAGACAAAGGAATAGCCTTCAATTGGCTTAGGTTTCATAGATATGATGTTCAGATTAATTTTAGACTCATGGTTCCACTTCTACCAATGCAAGATGAATGGGTGTGATGGTACCCGCCTTAAGTAGATGAATAGTTTAGTCTCATGGAGCAACGTTTATTGGGGTAAACGGCTAGTGAAAATCCTTATAGTTTTTAAGACCATGACACCTAGACAGTTATACAATTCTCATTCTTAACCTGATACGTCTGGACCGTGAAGTTTTTGTATGAACAGCAACTTTATGCAAGTAAAATGGCCAGTGCAGTTTTGTCGCCATCTACGGTGACACTCTGGATCAAGAAGACCCTGGCTATACCACTATTAATAGTGCCTCTTCTAGATTAG

mRNA sequence

ATGTCTCTTCCGCCGTCTCCCGCTTCCGGCCTCTCTCCAATGTCGGAACCCCATGAGGATGAAACTGGGATTTCTATGAAAGGTGTCAAAATCTCTGGACGACCGCTTTATCTTGATATGCAAGCTACATCCCCTGCTGACCCTCGGGTGCTCGATGCTAGGCTTCCTTATTATCTCTCTCGATGTGGTAATCCACCACTATCGGACGCATCTCTATGGTTGGGAATCGGATCATGCCGTCGAGTGCTCTTATTGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGCAGGTGGGTTTTGATATTACCTATTTACCGGTTGGGTCCGATGGTATTGTTGATTTGGAGAAGCTCCGTTCTGTGATTAGACCCGACACTGGCCTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTCATCCAGCCGTTAGAGGAAATTGGGAAAATTTGCAAGGAATTCAATGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGGAGATTCCAATTGATTTGGAGAAGTGGAATCAACTTTATGCAAGTAAAATGGCCAGTGCAGTTTTGTCGCCATCTACGGTGACACTCTGGATCAAGAAGACCCTGGCTATACCACTATTAATAGTGCCTCTTCTAGATTAG

Coding sequence (CDS)

ATGTCTCTTCCGCCGTCTCCCGCTTCCGGCCTCTCTCCAATGTCGGAACCCCATGAGGATGAAACTGGGATTTCTATGAAAGGTGTCAAAATCTCTGGACGACCGCTTTATCTTGATATGCAAGCTACATCCCCTGCTGACCCTCGGGTGCTCGATGCTAGGCTTCCTTATTATCTCTCTCGATGTGGTAATCCACCACTATCGGACGCATCTCTATGGTTGGGAATCGGATCATGCCGTCGAGTGCTCTTATTGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGCAGGTGGGTTTTGATATTACCTATTTACCGGTTGGGTCCGATGGTATTGTTGATTTGGAGAAGCTCCGTTCTGTGATTAGACCCGACACTGGCCTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTCATCCAGCCGTTAGAGGAAATTGGGAAAATTTGCAAGGAATTCAATGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGGAGATTCCAATTGATTTGGAGAAGTGGAATCAACTTTATGCAAGTAAAATGGCCAGTGCAGTTTTGTCGCCATCTACGGTGACACTCTGGATCAAGAAGACCCTGGCTATACCACTATTAATAGTGCCTCTTCTAGATTAG

Protein sequence

MSLPPSPASGLSPMSEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNPPLSDASLWLGIGSCRRVLLLTEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTVTLWIKKTLAIPLLIVPLLD

Homology

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BLAST of Carg00759 vs. NCBI nr
Match: KAG7034259.1 (Cysteine desulfurase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 437.6 bits (1124), Expect = 6.4e-119
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MSLPPSPASGLSPMSEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLS 60
           MSLPPSPASGLSPMSEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLS
Sbjct: 1   MSLPPSPASGLSPMSEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLS 60

Query: 61  RCGNPPLSDASLWLGIGSCRRVLLLTEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLE 120
           RCGNPPLSDASLWLGIGSCRRVLLLTEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLE
Sbjct: 61  RCGNPPLSDASLWLGIGSCRRVLLLTEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLE 120

Query: 121 KLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKW 180
           KLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKW
Sbjct: 121 KLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKW 180

Query: 181 NQLYASKMASAVLSPSTVTLWIKKTLAIPLLIVPLLD 218
           NQLYASKMASAVLSPSTVTLWIKKTLAIPLLIVPLLD
Sbjct: 181 NQLYASKMASAVLSPSTVTLWIKKTLAIPLLIVPLLD 217

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BLAST of Carg00759 vs. NCBI nr
Match: XP_011657914.1 (cysteine desulfurase, mitochondrial [Cucumis sativus])

HSP 1 Score: 261.9 bits (668), Expect = 4.8e-66
Identity = 140/229 (61.14%), Postives = 155/229 (67.69%), Query Frame = 0


Query: 15  SEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP--------- 74
           SEPHEDETGISMKGVKISGRPLYLDMQATSP DPRVLDA LPYY+SR GNP         
Sbjct: 39  SEPHEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGW 98

Query: 75  ------PLSDASLWLGIGSCRRVLLL------------------------------TEHK 134
                   + + +   IG+  + ++                               TEHK
Sbjct: 99  ESDHAVETARSQVAALIGASPKEIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHK 158

Query: 135 CVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLE 194
           CVLDSCRHLQQ+ GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+E
Sbjct: 159 CVLDSCRHLQQE-GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVE 218

Query: 195 EIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           EIGKICKEFNVPFHTDAAQALG+IPID+EKWN    S     +  P  +
Sbjct: 219 EIGKICKEFNVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKIYGPKGI 266

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BLAST of Carg00759 vs. NCBI nr
Match: KAE8647530.1 (hypothetical protein Csa_002991 [Cucumis sativus])

HSP 1 Score: 261.9 bits (668), Expect = 4.8e-66
Identity = 140/229 (61.14%), Postives = 155/229 (67.69%), Query Frame = 0


Query: 15  SEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP--------- 74
           SEPHEDETGISMKGVKISGRPLYLDMQATSP DPRVLDA LPYY+SR GNP         
Sbjct: 39  SEPHEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGW 98

Query: 75  ------PLSDASLWLGIGSCRRVLLL------------------------------TEHK 134
                   + + +   IG+  + ++                               TEHK
Sbjct: 99  ESDHAVETARSQVAALIGASPKEIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHK 158

Query: 135 CVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLE 194
           CVLDSCRHLQQ+ GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+E
Sbjct: 159 CVLDSCRHLQQE-GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVE 218

Query: 195 EIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           EIGKICKEFNVPFHTDAAQALG+IPID+EKWN    S     +  P  +
Sbjct: 219 EIGKICKEFNVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKIYGPKGI 266

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BLAST of Carg00759 vs. NCBI nr
Match: XP_008440458.1 (PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cysteine desulfurase [Cucumis melo var. makuwa] >TYK12780.1 cysteine desulfurase [Cucumis melo var. makuwa])

HSP 1 Score: 261.9 bits (668), Expect = 4.8e-66
Identity = 140/229 (61.14%), Postives = 155/229 (67.69%), Query Frame = 0


Query: 15  SEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP--------- 74
           SEPHEDETGISMKGVKISGRPLYLDMQATSP DPRVLDA LPYY+SR GNP         
Sbjct: 38  SEPHEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGW 97

Query: 75  ------PLSDASLWLGIGSCRRVLLL------------------------------TEHK 134
                   + + +   IG+  + ++                               TEHK
Sbjct: 98  ESDHAVETARSQVAALIGASPKEIVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHK 157

Query: 135 CVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLE 194
           CVLDSCRHLQQ+ GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+E
Sbjct: 158 CVLDSCRHLQQE-GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVE 217

Query: 195 EIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           EIGKICKEFNVPFHTDAAQALG+IPID+EKWN    S     +  P  +
Sbjct: 218 EIGKICKEFNVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKIYGPKGI 265

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BLAST of Carg00759 vs. NCBI nr
Match: XP_022132842.1 (cysteine desulfurase, mitochondrial [Momordica charantia])

HSP 1 Score: 260.8 bits (665), Expect = 1.1e-65
Identity = 142/236 (60.17%), Postives = 158/236 (66.95%), Query Frame = 0


Query: 8   ASGLSPMSEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP-- 67
           A+ ++  SEPHEDETGISMKGVKISGRPLYLDMQATSP DPRVLDA LPYYLSR GNP  
Sbjct: 31  AAVVTSPSEPHEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHS 90

Query: 68  -------------PLSDASLWLGIGSCRRVLLL--------------------------- 127
                          + + +   IG+  + ++                            
Sbjct: 91  RTHLYGWESDHAVETARSQVAALIGASPKEIVFTSGATESNNISVKGVMHFYREKKRHLI 150

Query: 128 ---TEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEI 187
              TEHKCVLDSCRHLQQ+ GFDITYLPVGSDGIVDLEKLRS IRP+TGLVSVMAVNNEI
Sbjct: 151 TTQTEHKCVLDSCRHLQQE-GFDITYLPVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEI 210

Query: 188 GVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           GVIQP+EEIGKICKEF VPFHTDAAQALG+IPID+EKWN    S     V  P  +
Sbjct: 211 GVIQPMEEIGKICKEFGVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKVYGPKGI 265

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BLAST of Carg00759 vs. ExPASy Swiss-Prot
Match: O49543 (Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 5.4e-52
Identity = 116/239 (48.54%), Postives = 142/239 (59.41%), Query Frame = 0


Query: 18  HEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP------------ 77
           +EDE+ I MKGV+ISGRPLYLDMQAT+P DPRV DA     +   GNP            
Sbjct: 38  YEDES-IMMKGVRISGRPLYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAE 97

Query: 78  --------------PLSDASLWLGIGSCRR-------------------VLLLTEHKCVL 137
                           S   +    G+                      +   TEHKCVL
Sbjct: 98  NAVENARNQVAKLIEASPKEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVL 157

Query: 138 DSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIG 197
           DSCRHLQQ+ GF++TYLPV +DG+VDLE LR  IRPDTGLVS+MAVNNEIGV+QP+EEIG
Sbjct: 158 DSCRHLQQE-GFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIG 217

Query: 198 KICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV-TLWIKKTLAIPL 211
            ICKE NVPFHTDAAQA+G+IP+D++KWN    S  A  +  P  V  L++++   I L
Sbjct: 218 MICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRL 274

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BLAST of Carg00759 vs. ExPASy Swiss-Prot
Match: Q54X04 (Probable cysteine desulfurase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=nfs1 PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 7.3e-41
Identity = 90/200 (45.00%), Postives = 119/200 (59.50%), Query Frame = 0


Query: 27  KGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP-PLSDASLWLG---------- 86
           +G+    +P+YLDMQ+T+P DPRVLDA LP Y    GNP   + A  W            
Sbjct: 43  RGIAGEKQPIYLDMQSTTPIDPRVLDAMLPLYTENYGNPHSKTHAYGWTSNDLVEDAREK 102

Query: 87  ----IGSCRRVLL------------------------------LTEHKCVLDSCRHLQQQ 146
               IG+  + ++                              +TEHKC+LDSCRHL+ +
Sbjct: 103 VSKIIGADSKEIIFTSGATESGNIAIKGVARFYKEKKNHIITTVTEHKCILDSCRHLEME 162

Query: 147 VGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVP 182
            GF +TYLPVG +G+VDLE L++ I P T LV++MAVNNEIGV+QP++EIGKIC+E  V 
Sbjct: 163 -GFKVTYLPVGENGLVDLELLKNTITPQTSLVTIMAVNNEIGVVQPIKEIGKICRENGVF 222

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BLAST of Carg00759 vs. ExPASy Swiss-Prot
Match: P0DN31 (Cysteine desulfurase, mitochondrial OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_05732-2 PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.7e-40
Identity = 85/195 (43.59%), Postives = 114/195 (58.46%), Query Frame = 0


Query: 34  RPLYLDMQATSPADPRVLDARLPYYLSRCGNP---------------------------- 93
           RP+YLDMQAT+P DPRVLDA LP+     GNP                            
Sbjct: 102 RPIYLDMQATTPTDPRVLDAMLPFLTGLYGNPHSRTHAYGWETEKATEQARSHVATLIGA 161

Query: 94  -----------------PLSDASLWLGIGSCRRVLLL--TEHKCVLDSCRHLQQQVGFDI 153
                             +   + + G    ++ ++   TEHKCVLDSCRHLQ + GF++
Sbjct: 162 DPKEIIFTSGATESNNMSIKGVARFFGRSGKKKHIITTQTEHKCVLDSCRHLQDE-GFEV 221

Query: 154 TYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTDA 182
           TYLPV S+G++ +E L + IRP+T LVS+M VNNEIGVIQP++EIG +C+   V FHTDA
Sbjct: 222 TYLPVQSNGLIKIEDLEAAIRPETALVSIMTVNNEIGVIQPMKEIGALCRSKKVFFHTDA 281

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BLAST of Carg00759 vs. ExPASy Swiss-Prot
Match: P87187 (Cysteine desulfurase, mitochondrial OS=Candida maltosa OX=5479 GN=SPL1 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.5e-38
Identity = 98/220 (44.55%), Postives = 121/220 (55.00%), Query Frame = 0


Query: 12  SPMSEPHEDETGISMKGVK-----ISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNPP 71
           SP   P  D + IS+K           RP+YLD+QAT+P DPRVLD  L +Y    GNP 
Sbjct: 57  SPSVHP-PDGSSISLKSATRDASLFGTRPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPH 116

Query: 72  LS-DASLW-----------------------------------LGIGSCRR--------- 131
            S  A  W                                   + I    R         
Sbjct: 117 SSTHAYGWETDKEVEKARGYIADVINADPKEIIFTSGATETNNMAIKGVPRFYKKTKKHI 176

Query: 132 VLLLTEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNE 182
           +   TEHKCVLDS RH+Q + GFDITYLPV S+G+++L+ L+  IR DT LVSVMAVNNE
Sbjct: 177 ITTQTEHKCVLDSARHMQDE-GFDITYLPVNSEGLINLDDLKKAIRKDTVLVSVMAVNNE 236

[Close]

BLAST of Carg00759 vs. ExPASy Swiss-Prot
Match: P87185 (Cysteine desulfurase, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=NFS1 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 3.4e-38
Identity = 90/193 (46.63%), Postives = 112/193 (58.03%), Query Frame = 0


Query: 34  RPLYLDMQATSPADPRVLDARLPYYLSRCGNPPLS-DASLW------------------- 93
           RP+YLD+QAT+P DPRVLD  L +Y    GNP  S  A  W                   
Sbjct: 87  RPIYLDVQATTPVDPRVLDKMLEFYTGLYGNPHSSTHAYGWETDKEVEKARTYIADVINA 146

Query: 94  ----------------LGIGSCRR---------VLLLTEHKCVLDSCRHLQQQVGFDITY 153
                           + I    R         +   TEHKCVLDS RH+Q + GF++TY
Sbjct: 147 DPKEIIFTSGATETNNMAIKGVPRFYKKTKKHIITTQTEHKCVLDSARHMQDE-GFEVTY 206

Query: 154 LPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIGKICKEFNVPFHTDAAQ 182
           LPV S+G+++L+ L+  IR DT LVS+MAVNNEIGVIQPL+EIGKIC+E  V FHTDAAQ
Sbjct: 207 LPVSSEGLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRENKVFFHTDAAQ 266

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BLAST of Carg00759 vs. ExPASy TrEMBL
Match: A0A5D3CMX0 (Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003690 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 2.3e-66
Identity = 140/229 (61.14%), Postives = 155/229 (67.69%), Query Frame = 0


Query: 15  SEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP--------- 74
           SEPHEDETGISMKGVKISGRPLYLDMQATSP DPRVLDA LPYY+SR GNP         
Sbjct: 38  SEPHEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGW 97

Query: 75  ------PLSDASLWLGIGSCRRVLLL------------------------------TEHK 134
                   + + +   IG+  + ++                               TEHK
Sbjct: 98  ESDHAVETARSQVAALIGASPKEIVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHK 157

Query: 135 CVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLE 194
           CVLDSCRHLQQ+ GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+E
Sbjct: 158 CVLDSCRHLQQE-GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVE 217

Query: 195 EIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           EIGKICKEFNVPFHTDAAQALG+IPID+EKWN    S     +  P  +
Sbjct: 218 EIGKICKEFNVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKIYGPKGI 265

[Close]

BLAST of Carg00759 vs. ExPASy TrEMBL
Match: A0A0A0KIM0 (Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G497010 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 2.3e-66
Identity = 140/229 (61.14%), Postives = 155/229 (67.69%), Query Frame = 0


Query: 15  SEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP--------- 74
           SEPHEDETGISMKGVKISGRPLYLDMQATSP DPRVLDA LPYY+SR GNP         
Sbjct: 39  SEPHEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGW 98

Query: 75  ------PLSDASLWLGIGSCRRVLLL------------------------------TEHK 134
                   + + +   IG+  + ++                               TEHK
Sbjct: 99  ESDHAVETARSQVAALIGASPKEIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHK 158

Query: 135 CVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLE 194
           CVLDSCRHLQQ+ GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+E
Sbjct: 159 CVLDSCRHLQQE-GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVE 218

Query: 195 EIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           EIGKICKEFNVPFHTDAAQALG+IPID+EKWN    S     +  P  +
Sbjct: 219 EIGKICKEFNVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKIYGPKGI 266

[Close]

BLAST of Carg00759 vs. ExPASy TrEMBL
Match: A0A1S3B0Q9 (Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 2.3e-66
Identity = 140/229 (61.14%), Postives = 155/229 (67.69%), Query Frame = 0


Query: 15  SEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP--------- 74
           SEPHEDETGISMKGVKISGRPLYLDMQATSP DPRVLDA LPYY+SR GNP         
Sbjct: 38  SEPHEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGW 97

Query: 75  ------PLSDASLWLGIGSCRRVLLL------------------------------TEHK 134
                   + + +   IG+  + ++                               TEHK
Sbjct: 98  ESDHAVETARSQVAALIGASPKEIVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHK 157

Query: 135 CVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLE 194
           CVLDSCRHLQQ+ GF+ITYLPVGSDGIVDLEKLRS IRPDTGLVSVMAVNNEIGVIQP+E
Sbjct: 158 CVLDSCRHLQQE-GFEITYLPVGSDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVE 217

Query: 195 EIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           EIGKICKEFNVPFHTDAAQALG+IPID+EKWN    S     +  P  +
Sbjct: 218 EIGKICKEFNVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKIYGPKGI 265

[Close]

BLAST of Carg00759 vs. ExPASy TrEMBL
Match: A0A6J1BUZ1 (Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 5.2e-66
Identity = 142/236 (60.17%), Postives = 158/236 (66.95%), Query Frame = 0


Query: 8   ASGLSPMSEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP-- 67
           A+ ++  SEPHEDETGISMKGVKISGRPLYLDMQATSP DPRVLDA LPYYLSR GNP  
Sbjct: 31  AAVVTSPSEPHEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHS 90

Query: 68  -------------PLSDASLWLGIGSCRRVLLL--------------------------- 127
                          + + +   IG+  + ++                            
Sbjct: 91  RTHLYGWESDHAVETARSQVAALIGASPKEIVFTSGATESNNISVKGVMHFYREKKRHLI 150

Query: 128 ---TEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEI 187
              TEHKCVLDSCRHLQQ+ GFDITYLPVGSDGIVDLEKLRS IRP+TGLVSVMAVNNEI
Sbjct: 151 TTQTEHKCVLDSCRHLQQE-GFDITYLPVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEI 210

Query: 188 GVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           GVIQP+EEIGKICKEF VPFHTDAAQALG+IPID+EKWN    S     V  P  +
Sbjct: 211 GVIQPMEEIGKICKEFGVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKVYGPKGI 265

[Close]

BLAST of Carg00759 vs. ExPASy TrEMBL
Match: A0A6J1HHQ0 (Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111463622 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.2e-65
Identity = 141/238 (59.24%), Postives = 159/238 (66.81%), Query Frame = 0


Query: 6   SPASGLSPMSEPHEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP 65
           S A+ ++  SE HEDE+GISMKGVKISGRPLYLDMQATSP DPRVLDA LPYYLSR GNP
Sbjct: 28  STAAAVATSSELHEDESGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYLSRYGNP 87

Query: 66  ---------------PLSDASLWLGIGSCRRVLLL------------------------- 125
                            + + +   IG+  + ++                          
Sbjct: 88  HSRTHLYGWESDHAVETARSQVAALIGASPKEIVFTSGATESNNISVKGVMHFYKEKKKH 147

Query: 126 -----TEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNN 185
                TEHKCVLDSCRHLQQ+ GFDITYLPVG+DGIVDLEKLRS IRPDTGLVS+MAVNN
Sbjct: 148 VITTQTEHKCVLDSCRHLQQE-GFDITYLPVGTDGIVDLEKLRSAIRPDTGLVSIMAVNN 207

Query: 186 EIGVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           EIGVIQP+EEIGKICKEFNVPFHTDAAQALG+IPID+EKWN    S     V  P  +
Sbjct: 208 EIGVIQPVEEIGKICKEFNVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKVYGPKGI 264

[Close]

BLAST of Carg00759 vs. TAIR 10
Match: AT5G65720.1 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 205.7 bits (522), Expect = 3.8e-53
Identity = 116/239 (48.54%), Postives = 142/239 (59.41%), Query Frame = 0


Query: 18  HEDETGISMKGVKISGRPLYLDMQATSPADPRVLDARLPYYLSRCGNP------------ 77
           +EDE+ I MKGV+ISGRPLYLDMQAT+P DPRV DA     +   GNP            
Sbjct: 38  YEDES-IMMKGVRISGRPLYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAE 97

Query: 78  --------------PLSDASLWLGIGSCRR-------------------VLLLTEHKCVL 137
                           S   +    G+                      +   TEHKCVL
Sbjct: 98  NAVENARNQVAKLIEASPKEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVL 157

Query: 138 DSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVIQPLEEIG 197
           DSCRHLQQ+ GF++TYLPV +DG+VDLE LR  IRPDTGLVS+MAVNNEIGV+QP+EEIG
Sbjct: 158 DSCRHLQQE-GFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIG 217

Query: 198 KICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV-TLWIKKTLAIPL 211
            ICKE NVPFHTDAAQA+G+IP+D++KWN    S  A  +  P  V  L++++   I L
Sbjct: 218 MICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRL 274

[Close]

BLAST of Carg00759 vs. TAIR 10
Match: AT5G65720.2 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 173.7 bits (439), Expect = 1.6e-43
Identity = 83/126 (65.87%), Postives = 101/126 (80.16%), Query Frame = 0


Query: 86  TEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNEIGVI 145
           TEHKCVLDSCRHLQQ+ GF++TYLPV +DG+VDLE LR  IRPDTGLVS+MAVNNEIGV+
Sbjct: 22  TEHKCVLDSCRHLQQE-GFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVV 81

Query: 146 QPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV-TLWIKK 205
           QP+EEIG ICKE NVPFHTDAAQA+G+IP+D++KWN    S  A  +  P  V  L++++
Sbjct: 82  QPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRR 141

Query: 206 TLAIPL 211
              I L
Sbjct: 142 RPRIRL 146

[Close]

BLAST of Carg00759 vs. TAIR 10
Match: AT1G08490.1 (chloroplastic NIFS-like cysteine desulfurase )

HSP 1 Score: 55.8 bits (133), Expect = 4.9e-08
Identity = 29/117 (24.79%), Postives = 58/117 (49.57%), Query Frame = 0


Query: 82  VLLLTEHKCVLDSCRHLQQQVGFDITYLPVGSDGIVDLEKLRSVIRPDTGLVSVMAVNNE 141
           +L + EH   +   + + Q+ G  + ++ +  D + D+ KLR +I P T LV+V  V+N 
Sbjct: 166 ILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNV 225

Query: 142 IGVIQPLEEIGKICKEFNVPFHTDAAQALGEIPIDLEKWNQLYASKMASAVLSPSTV 199
           +    P+EEI     +       DA Q++  + +D++K N  +    +  +  P+ +
Sbjct: 226 LASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGI 282

The following BLAST results are available for this feature:

BLAST of Carg00759 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7034259.1	6.4e-119	100.00	Cysteine desulfurase, mitochondrial, partial [Cucurbita argyrosperma subsp. argy...	[more]
XP_011657914.1	4.8e-66	61.14	cysteine desulfurase, mitochondrial [Cucumis sativus]	[more]
KAE8647530.1	4.8e-66	61.14	hypothetical protein Csa_002991 [Cucumis sativus]	[more]
XP_008440458.1	4.8e-66	61.14	PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cyst...	[more]
XP_022132842.1	1.1e-65	60.17	cysteine desulfurase, mitochondrial [Momordica charantia]	[more]

BLAST of Carg00759 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49543	5.4e-52	48.54	Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=...	[more]
Q54X04	7.3e-41	45.00	Probable cysteine desulfurase, mitochondrial OS=Dictyostelium discoideum OX=4468...	[more]
P0DN31	4.7e-40	43.59	Cysteine desulfurase, mitochondrial OS=Arthroderma benhamiae (strain ATCC MYA-46...	[more]
P87187	1.5e-38	44.55	Cysteine desulfurase, mitochondrial OS=Candida maltosa OX=5479 GN=SPL1 PE=3 SV=1	[more]
P87185	3.4e-38	46.63	Cysteine desulfurase, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MY...	[more]

BLAST of Carg00759 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CMX0	2.3e-66	61.14	Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255...	[more]
A0A0A0KIM0	2.3e-66	61.14	Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G497010 PE=3 SV=1	[more]
A0A1S3B0Q9	2.3e-66	61.14	Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1	[more]
A0A6J1BUZ1	5.2e-66	60.17	Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1	[more]
A0A6J1HHQ0	1.2e-65	59.24	Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111463622 PE=3 SV=1	[more]

BLAST of Carg00759 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65720.1	3.8e-53	48.54	nitrogen fixation S (NIFS)-like 1	[more]
AT5G65720.2	1.6e-43	65.87	nitrogen fixation S (NIFS)-like 1	[more]
AT1G08490.1	4.9e-08	24.79	chloroplastic NIFS-like cysteine desulfurase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000192	Aminotransferase class V domain	PFAM	PF00266	Aminotran_5	coord: 85..182 e-value: 7.3E-24 score: 84.4
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 84..203 e-value: 2.2E-39 score: 137.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR11601	CYSTEINE DESULFURYLASE FAMILY MEMBER	coord: 26..66
None	No IPR available	PANTHER	PTHR11601	CYSTEINE DESULFURYLASE FAMILY MEMBER	coord: 86..195
IPR010240	Cysteine desulfurase IscS	PANTHER	PTHR11601:SF34	CYSTEINE DESULFURASE, MITOCHONDRIAL	coord: 86..195
IPR010240	Cysteine desulfurase IscS	PANTHER	PTHR11601:SF34	CYSTEINE DESULFURASE, MITOCHONDRIAL	coord: 26..66
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 34..182

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00759-RA	Carg00759-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044571	[2Fe-2S] cluster assembly
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0031071	cysteine desulfurase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0003824	catalytic activity