Carg00701 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00701
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Alpha/beta hydrolase-1
Location	Carg_Chr03: 6318242 .. 6320159 (+)
RNA-Seq Expression	Carg00701
Synteny	Carg00701

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCCATTTCTCATACAATTTTCAGGATTTCAATTTGTTCTTGTTCTTCAGTTGGGTTATTTTGATCCTGTTCTAGTATAATGGCTCAGTGTTTTAGGTATGGATCGATCATGGAGGCATGGTACCGTTCCTGTTTCTCTAGAGTGGGTCTTAGATCCGCCACCACCGATCTCGGAAACGGCACCGTCATGCACTGCTGGATCCCCAAAACCCGCAGAGAAACTAAGCCCAATTTGGTTCTGATCCATGGCATGGGAGCAAATGCAGTGTGGCAGTGGAACAAATTCGTCCGCCCACTCATCGCCCACTTCAACATCTATGTCCCTGACTTGGTCTTCTTTGGGGAATCCTACACCACCCTTTCCGATCGCTCCGAGGCATTTCAGGCTCGGTGTGTGATGGGTGTTTTAGATGCTCATGGAGTACGGACTACAAACGCGGTGGGGATAAGCTATGGTGGGTTTGTGGCTTATAGCATGGCTGCACAGTTCCCTGATAGGATGGAGAAATTGGTGCTCTGTTGTACTGGCGTTTGCTTGGAAGATAAAGATATGGAAGATGGGATGTTCCAGGTGAAGTCCGTCGAGGAGGCAGTGAGCGTCTTGCTTCCTCAAACGCCGGAGAAGTTGAAGGAAATGATCCGTATTGCGTTTTTCAAGCCTATTAGAATTGGACCCTCTTGCTTGGTCAACGACCTTATCGATGTAAGTAATTCATCATTCAGTTTATTTCTCTGATTTTTCTGACTAACATTTGCATCTTATGAATGGGTTTCTGCCAAATTCATGTCTTTCTTCGTTATCTCTTTGTGCCAAGCATTGATCTGAAACAAGTTCGGTTTTGTTTCAAGGATTTTGAAAATGACTATTGGTTGTGCTGTAGGAATTGTGTACCGAGTACCGTGAACAAAAGAAGGAGCTGATCCAAGCATTGCACAAGGAAAGGAAGTTCTCAGATCTTCCCAAGATTACTCAGGTTTGTCTGATTATATATTAGAAGATTTCAGAGTTTGGGTTTGGTTTTAAAATTTGTTCCACTCTTCTGACTGGCTGATTAACAATGGATGCAGCCTACACTGATAATGTGGGGAGAAAAGGACTTGGTATTCCCCATGGAATTGGCACACAGACTCAAAAGGTGAATCTGATGAACTTGTGTTAATTCTTGTTTGGGTTTATGTCTTTATTCTGGTGTGTTTCGAGTTAAGAACTGAAAATTTAGTTGGTTCTTTTGGGTAATCTCTTCAATTTTGCTATTGATTTCTACAGCCACATAGGTGATGGTGCGGAACTAGTGGTGATAAAGAAAGCAGGGCATGCTTTAAATATAGAGAAGCCCAAGGAGATGAACAAGCTTATTCAGTGTTTCCTTGTTGATGCTCACCAATCAAGCCAGGCCAAAATTCACCACCAAAATGACCCCAAATCTGAGTGAAATACTGAAATTTAAAATGGATCTCCCCCGGAGCTATCAATTTCTCTGCTTCAGTCCCAACTTCATGGTGGCCTACAGCTGCTGCTGCTGCTTCTACTTTTATGTTATAAAATTAGTGTATATTACCAATTTGTAGTTAATGTAATGAAGTTTTATAATAGTGAAAAGGTGAGGAGCTTTGTGAAGATTCTACTGTATACGAACTGAACTGTTGTTTAGTGTTGCATAAGAGTCGTAAGTCCTGCTTCAGTCCATATCGCATTTTAGGCCTGCTCCGTTCTCTTATTATCCATTGGTTCATTGCACTGGGTTCCTCTCTGCTCTATCAACTGAACTAAGATCGGTGTGTTGTTCACATAAACCACGTTGTAATGAAGAATGGAATCGAATGGAATCAAAAGTTGTCAAGTTGATATTATTATAATACAAGAGGGTACGATTGAAAAAATATTATAATCAGCCAGACTTTCAAGTTTCAACACAAA

mRNA sequence

TTCCATTTCTCATACAATTTTCAGGATTTCAATTTGTTCTTGTTCTTCAGTTGGGTTATTTTGATCCTGTTCTAGTATAATGGCTCAGTGTTTTAGGTATGGATCGATCATGGAGGCATGGTACCGTTCCTGTTTCTCTAGAGTGGGTCTTAGATCCGCCACCACCGATCTCGGAAACGGCACCGTCATGCACTGCTGGATCCCCAAAACCCGCAGAGAAACTAAGCCCAATTTGGTTCTGATCCATGGCATGGGAGCAAATGCAGTGTGGCAGTGGAACAAATTCGTCCGCCCACTCATCGCCCACTTCAACATCTATGTCCCTGACTTGGTCTTCTTTGGGGAATCCTACACCACCCTTTCCGATCGCTCCGAGGCATTTCAGGCTCGGTGTGTGATGGGTGTTTTAGATGCTCATGGAGTACGGACTACAAACGCGGTGGGGATAAGCTATGGTGGGTTTGTGGCTTATAGCATGGCTGCACAGTTCCCTGATAGGATGGAGAAATTGGTGCTCTGTTGTACTGGCGTTTGCTTGGAAGATAAAGATATGGAAGATGGGATGTTCCAGGTGAAGTCCGTCGAGGAGGCAGTGAGCGTCTTGCTTCCTCAAACGCCGGAGAAGTTGAAGGAAATGATCCGTATTGCGTTTTTCAAGCCTATTAGAATTGGACCCTCTTGCTTGGTCAACGACCTTATCGATGAATTGTGTACCGAGTACCGTGAACAAAAGAAGGAGCTGATCCAAGCATTGCACAAGGAAAGGAAGTTCTCAGATCTTCCCAAGATTACTCAGCCTACACTGATAATGTGGGGAGAAAAGGACTTGGTATTCCCCATGGAATTGGCACACAGACTCAAAAGCCACATAGGTGATGGTGCGGAACTAGTGGTGATAAAGAAAGCAGGGCATGCTTTAAATATAGAGAAGCCCAAGGAGATGAACAAGCTTATTCAGTGTTTCCTTGTTGATGCTCACCAATCAAGCCAGGCCAAAATTCACCACCAAAATGACCCCAAATCTGAGTGAAATACTGAAATTTAAAATGGATCTCCCCCGGAGCTATCAATTTCTCTGCTTCAGTCCCAACTTCATGGTGGCCTACAGCTGCTGCTGCTGCTTCTACTTTTATGTTATAAAATTAGTGTATATTACCAATTTGTAGTTAATGTAATGAAGTTTTATAATAGTGAAAAGGTGAGGAGCTTTGTGAAGATTCTACTGTATACGAACTGAACTGTTGTTTAGTGTTGCATAAGAGTCGTAAGTCCTGCTTCAGTCCATATCGCATTTTAGGCCTGCTCCGTTCTCTTATTATCCATTGGTTCATTGCACTGGGTTCCTCTCTGCTCTATCAACTGAACTAAGATCGGTGTGTTGTTCACATAAACCACGTTGTAATGAAGAATGGAATCGAATGGAATCAAAAGTTGTCAAGTTGATATTATTATAATACAAGAGGGTACGATTGAAAAAATATTATAATCAGCCAGACTTTCAAGTTTCAACACAAA

Coding sequence (CDS)

ATGGCTCAGTGTTTTAGGTATGGATCGATCATGGAGGCATGGTACCGTTCCTGTTTCTCTAGAGTGGGTCTTAGATCCGCCACCACCGATCTCGGAAACGGCACCGTCATGCACTGCTGGATCCCCAAAACCCGCAGAGAAACTAAGCCCAATTTGGTTCTGATCCATGGCATGGGAGCAAATGCAGTGTGGCAGTGGAACAAATTCGTCCGCCCACTCATCGCCCACTTCAACATCTATGTCCCTGACTTGGTCTTCTTTGGGGAATCCTACACCACCCTTTCCGATCGCTCCGAGGCATTTCAGGCTCGGTGTGTGATGGGTGTTTTAGATGCTCATGGAGTACGGACTACAAACGCGGTGGGGATAAGCTATGGTGGGTTTGTGGCTTATAGCATGGCTGCACAGTTCCCTGATAGGATGGAGAAATTGGTGCTCTGTTGTACTGGCGTTTGCTTGGAAGATAAAGATATGGAAGATGGGATGTTCCAGGTGAAGTCCGTCGAGGAGGCAGTGAGCGTCTTGCTTCCTCAAACGCCGGAGAAGTTGAAGGAAATGATCCGTATTGCGTTTTTCAAGCCTATTAGAATTGGACCCTCTTGCTTGGTCAACGACCTTATCGATGAATTGTGTACCGAGTACCGTGAACAAAAGAAGGAGCTGATCCAAGCATTGCACAAGGAAAGGAAGTTCTCAGATCTTCCCAAGATTACTCAGCCTACACTGATAATGTGGGGAGAAAAGGACTTGGTATTCCCCATGGAATTGGCACACAGACTCAAAAGCCACATAGGTGATGGTGCGGAACTAGTGGTGATAAAGAAAGCAGGGCATGCTTTAAATATAGAGAAGCCCAAGGAGATGAACAAGCTTATTCAGTGTTTCCTTGTTGATGCTCACCAATCAAGCCAGGCCAAAATTCACCACCAAAATGACCCCAAATCTGAGTGA

Protein sequence

MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQSSQAKIHHQNDPKSE

Homology

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BLAST of Carg00701 vs. NCBI nr
Match: XP_022950912.1 (uncharacterized protein LOC111453865 [Cucurbita moschata] >XP_022978531.1 uncharacterized protein LOC111478485 [Cucurbita maxima] >KAG6604037.1 Monoacylglycerol lipase ABHD6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034202.1 Monoacylglycerol lipase ABHD6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 654.8 bits (1688), Expect = 3.7e-184
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA 60
           MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA
Sbjct: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA 60

Query: 61  NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA 120
           NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA
Sbjct: 61  NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA 120

Query: 121 VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP 180
           VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP
Sbjct: 121 VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP 180

Query: 181 EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP 240
           EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP
Sbjct: 181 EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP 240

Query: 241 TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH 300
           TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH
Sbjct: 241 TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH 300

Query: 301 QSSQAKIHHQNDPKSE 317
           QSSQAKIHHQNDPKSE
Sbjct: 301 QSSQAKIHHQNDPKSE 316

[Close]

BLAST of Carg00701 vs. NCBI nr
Match: XP_023543119.1 (uncharacterized protein LOC111803081 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 652.9 bits (1683), Expect = 1.4e-183
Identity = 315/316 (99.68%), Postives = 315/316 (99.68%), Query Frame = 0


Query: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA 60
           MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA
Sbjct: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA 60

Query: 61  NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA 120
           NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRT NA
Sbjct: 61  NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTANA 120

Query: 121 VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP 180
           VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP
Sbjct: 121 VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP 180

Query: 181 EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP 240
           EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP
Sbjct: 181 EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP 240

Query: 241 TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH 300
           TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH
Sbjct: 241 TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH 300

Query: 301 QSSQAKIHHQNDPKSE 317
           QSSQAKIHHQNDPKSE
Sbjct: 301 QSSQAKIHHQNDPKSE 316

[Close]

BLAST of Carg00701 vs. NCBI nr
Match: XP_008440587.1 (PREDICTED: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Cucumis melo] >KAA0036310.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Cucumis melo var. makuwa] >TYK12704.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Cucumis melo var. makuwa])

HSP 1 Score: 610.5 bits (1573), Expect = 8.0e-171
Identity = 292/314 (92.99%), Postives = 305/314 (97.13%), Query Frame = 0


Query: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62
           QCF YGSIMEAWYRSCFSRVGL+SATTDLGNGTVMHCWIPKT ++TKPNLVLIHGMGANA
Sbjct: 4   QCFSYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPKQTKPNLVLIHGMGANA 63

Query: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 122
           +WQWN+FVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG
Sbjct: 64  MWQWNQFVRPLITHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 123

Query: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182
           ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLED+DMEDGMFQVKSVEEAVSVLLPQ+PEK
Sbjct: 124 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQSPEK 183

Query: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242
           LKEMI+IAFFKPIRIGPSC+VNDLIDELCTEYREQKKELIQALHKERK S+LPKIT PTL
Sbjct: 184 LKEMIKIAFFKPIRIGPSCVVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITNPTL 243

Query: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302
           IMWGEKDLVFPMELAHRLK HIG+GAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA  S
Sbjct: 244 IMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 303

Query: 303 SQAKIHHQNDPKSE 317
           ++AKIHHQND KSE
Sbjct: 304 TKAKIHHQNDLKSE 317

[Close]

BLAST of Carg00701 vs. NCBI nr
Match: XP_004143463.1 (uncharacterized protein LOC101208157 [Cucumis sativus] >KGN48741.1 hypothetical protein Csa_003107 [Cucumis sativus])

HSP 1 Score: 608.6 bits (1568), Expect = 3.0e-170
Identity = 290/314 (92.36%), Postives = 305/314 (97.13%), Query Frame = 0


Query: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62
           QCF YGSIM+AWYRSCFSRVGL SATTDLGNGTVMHCWIPKT +ETKPNLVLIHGMGANA
Sbjct: 4   QCFSYGSIMDAWYRSCFSRVGLTSATTDLGNGTVMHCWIPKTPKETKPNLVLIHGMGANA 63

Query: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 122
           +WQWN+FVRPL++HFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG
Sbjct: 64  MWQWNQFVRPLVSHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 123

Query: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182
           +SYGGFVAYSMAAQFPDR+EKLVLCCTGVCLED+DMEDGMFQVKSVEEAVSVLLPQ+PEK
Sbjct: 124 VSYGGFVAYSMAAQFPDRVEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQSPEK 183

Query: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242
           LKEMI+IAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERK S+LPKIT PTL
Sbjct: 184 LKEMIKIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITNPTL 243

Query: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302
           IMWGEKDLVFPMELAHRLK HIG+GAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA  S
Sbjct: 244 IMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 303

Query: 303 SQAKIHHQNDPKSE 317
           ++AKIHHQND KSE
Sbjct: 304 TKAKIHHQNDLKSE 317

[Close]

BLAST of Carg00701 vs. NCBI nr
Match: XP_038882658.1 (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Benincasa hispida])

HSP 1 Score: 598.6 bits (1542), Expect = 3.2e-167
Identity = 286/314 (91.08%), Postives = 303/314 (96.50%), Query Frame = 0


Query: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62
           QCFRYGSIM++WYRSCFSRVGL+SATTDLGNGTVMHCWIPK  ++TKPNLVLIHGMGANA
Sbjct: 4   QCFRYGSIMDSWYRSCFSRVGLKSATTDLGNGTVMHCWIPKNPKQTKPNLVLIHGMGANA 63

Query: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 122
           +WQWN+F RPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVRTTNAVG
Sbjct: 64  MWQWNQFARPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRTTNAVG 123

Query: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182
           ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLED+DMEDGMFQVKSV+EAVSVLLPQ+PEK
Sbjct: 124 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDQDMEDGMFQVKSVDEAVSVLLPQSPEK 183

Query: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242
           LKEMI+IAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERK S+LPKITQPTL
Sbjct: 184 LKEMIQIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITQPTL 243

Query: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302
           I+WGEKDLVFPMELAHRLK HIGD AELVVIKKAGHALNIEKPKEMNKLIQCFLVD+  S
Sbjct: 244 IIWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDSIPS 303

Query: 303 SQAKIHHQNDPKSE 317
           ++AKIHHQ+D KSE
Sbjct: 304 TKAKIHHQSDLKSE 317

[Close]

BLAST of Carg00701 vs. ExPASy Swiss-Prot
Match: Q52532 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Pseudomonas sp. OX=306 GN=mhpC PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.6e-15
Identity = 70/256 (27.34%), Postives = 117/256 (45.70%), Query Frame = 0


Query: 52  LVLIHGMGANAVWQWNKFVRPLIAH----FNIYVPDLVFFGESYTTLSDRSEAFQARCVM 111
           +V+ HG G  A   W+ F R + A     + + + D   F +SY  ++   +   A+   
Sbjct: 36  VVMFHGSGPGAS-GWSNFHRNVDAFVDAGYRVLLIDSPGFNKSYPIVTKSRDGAYAQAAK 95

Query: 112 GVLDAHGVRTTNAVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKS 171
           GV+D  G++  + +G S GG  A  MA  +P+ + KLV+   G          G      
Sbjct: 96  GVMDKLGIKRAHMIGNSMGGATAMRMAVDYPEMVGKLVMMGGG-------SVGGSTTTPM 155

Query: 172 VEEAVSVL--LPQTP--EKLKEMIRIAFFKPIRIGPSCLVNDLIDELC--TEYREQKKEL 231
             E + +L  L + P  E L++M+ I  + P  +    L+N   + +    E+     E 
Sbjct: 156 PTEGLKLLQGLYRNPSMENLRKMLDIFVYAPSTLTEE-LINGRFENMMRRPEHLTNFVES 215

Query: 232 IQALHKERKFSD-LPKITQPTLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHAL 291
           ++A      ++  LP +T PT+I+WG  D   P++L  R+   + D AEL V  K GH  
Sbjct: 216 LKASGGRANYAHLLPTLTMPTMIIWGRDDRFVPLDLGLRMLWGMPD-AELHVFSKCGHWA 275

Query: 292 NIEKPKEMNKLIQCFL 297
             E   + N+L+  FL
Sbjct: 276 QWEHADKFNQLVLNFL 281

[Close]

BLAST of Carg00701 vs. ExPASy Swiss-Prot
Match: Q13QH4 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Paraburkholderia xenovorans (strain LB400) OX=266265 GN=mhpC PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.5e-14
Identity = 68/258 (26.36%), Postives = 124/258 (48.06%), Query Frame = 0


Query: 52  LVLIHGMGANAV-W-QWNKFVRPLI-AHFNIYVPDLVFFGESYTTLSDRSEA-FQARCVM 111
           +V++HG G  A  W  +N+ V PL+ A + + + D + + +S   + D S +   AR + 
Sbjct: 40  VVMLHGSGPGASGWANFNRNVEPLVAAGYRVILLDCLGWSKSDPVVCDGSRSELNARSLK 99

Query: 112 GVLDAHGVRTTNAVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKS 171
           G+LDA  +   + +G S GG  A + A   P R+ KLVL   G     + +       + 
Sbjct: 100 GLLDALDIERVHIIGNSMGGHSAVAFALANPQRVGKLVLMGGGTGGPSQFVP---MPTEG 159

Query: 172 VEEAVSVLLPQTPEKLKEMIRIAFFKPIRIGPSCLVNDL----IDELCTEYREQKKELIQ 231
           ++    +    T + LK M+ +  F       S L +DL    +D +    R+  +  ++
Sbjct: 160 IKLLQGLYREPTIDNLKRMMNVFVF-----DASALTDDLMQARLDNMLAR-RDHLENFVK 219

Query: 232 AL-HKERKFSD----LPKITQPTLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGH 291
           +L    ++F+D    L +I  PTL++WG  D   PM++  RL + +   AE+ +  + GH
Sbjct: 220 SLAANPKQFNDFGPRLGEIAAPTLVIWGRDDRFVPMDVGLRLVAGM-QNAEMHIFSRCGH 279

Query: 292 ALNIEKPKEMNKLIQCFL 297
               E  ++ N+++  FL
Sbjct: 280 WAQWEHAEKFNRMVVDFL 287

[Close]

BLAST of Carg00701 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 8.4e-14
Identity = 68/261 (26.05%), Postives = 119/261 (45.59%), Query Frame = 0


Query: 50  PNLVLIHGMGANAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGV 109
           P ++LIHG+G N+   WN     L   F +  PDL+  G+S    +D S A  A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 110 LDAHGVRTTNAVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVE 169
           L    +     VG S GG VA   A QFP  +++L+L   G   +D ++   +  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 170 EAVSVL-LPQTPEKLKEMIRIAFFKPIRIGPSCLVNDL------IDEL-----CTEYREQ 229
           EA+++L LP     ++   RI       IG + L +DL      +D+L        +   
Sbjct: 158 EAMALLRLPLVLPAVQIAGRIV---GKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRT 217

Query: 230 KKELIQALHKERKFSDLPKITQ--PTLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKK 289
            + ++    +     D   +T+  P  I+WG KD+V P+  AH   + +  G++L + + 
Sbjct: 218 LRAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDVVLPVRHAHMAHAAM-PGSQLEIFEG 277

Query: 290 AGHALNIEKPKEMNKLIQCFL 297
           +GH    + P     +++ F+
Sbjct: 278 SGHFPFHDDPARFIDIVERFM 293

[Close]

BLAST of Carg00701 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 8.4e-14
Identity = 68/261 (26.05%), Postives = 119/261 (45.59%), Query Frame = 0


Query: 50  PNLVLIHGMGANAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGV 109
           P ++LIHG+G N+   WN     L   F +  PDL+  G+S    +D S A  A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 110 LDAHGVRTTNAVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVE 169
           L    +     VG S GG VA   A QFP  +++L+L   G   +D ++   +  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 170 EAVSVL-LPQTPEKLKEMIRIAFFKPIRIGPSCLVNDL------IDEL-----CTEYREQ 229
           EA+++L LP     ++   RI       IG + L +DL      +D+L        +   
Sbjct: 158 EAMALLRLPLVLPAVQIAGRIV---GKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRT 217

Query: 230 KKELIQALHKERKFSDLPKITQ--PTLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKK 289
            + ++    +     D   +T+  P  I+WG KD+V P+  AH   + +  G++L + + 
Sbjct: 218 LRAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDVVLPVRHAHMAHAAM-PGSQLEIFEG 277

Query: 290 AGHALNIEKPKEMNKLIQCFL 297
           +GH    + P     +++ F+
Sbjct: 278 SGHFPFHDDPARFIDIVERFM 293

[Close]

BLAST of Carg00701 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 8.4e-14
Identity = 68/261 (26.05%), Postives = 119/261 (45.59%), Query Frame = 0


Query: 50  PNLVLIHGMGANAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGV 109
           P ++LIHG+G N+   WN     L   F +  PDL+  G+S    +D S A  A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 110 LDAHGVRTTNAVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVE 169
           L    +     VG S GG VA   A QFP  +++L+L   G   +D ++   +  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 170 EAVSVL-LPQTPEKLKEMIRIAFFKPIRIGPSCLVNDL------IDEL-----CTEYREQ 229
           EA+++L LP     ++   RI       IG + L +DL      +D+L        +   
Sbjct: 158 EAMALLRLPLVLPAVQIAGRIV---GKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRT 217

Query: 230 KKELIQALHKERKFSDLPKITQ--PTLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKK 289
            + ++    +     D   +T+  P  I+WG KD+V P+  AH   + +  G++L + + 
Sbjct: 218 LRAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDVVLPVRHAHMAHAAM-PGSQLEIFEG 277

Query: 290 AGHALNIEKPKEMNKLIQCFL 297
           +GH    + P     +++ F+
Sbjct: 278 SGHFPFHDDPARFIDIVERFM 293

[Close]

BLAST of Carg00701 vs. ExPASy TrEMBL
Match: A0A6J1IMX6 (uncharacterized protein LOC111478485 OS=Cucurbita maxima OX=3661 GN=LOC111478485 PE=4 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.8e-184
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA 60
           MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA
Sbjct: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA 60

Query: 61  NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA 120
           NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA
Sbjct: 61  NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA 120

Query: 121 VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP 180
           VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP
Sbjct: 121 VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP 180

Query: 181 EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP 240
           EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP
Sbjct: 181 EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP 240

Query: 241 TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH 300
           TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH
Sbjct: 241 TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH 300

Query: 301 QSSQAKIHHQNDPKSE 317
           QSSQAKIHHQNDPKSE
Sbjct: 301 QSSQAKIHHQNDPKSE 316

[Close]

BLAST of Carg00701 vs. ExPASy TrEMBL
Match: A0A6J1GG71 (uncharacterized protein LOC111453865 OS=Cucurbita moschata OX=3662 GN=LOC111453865 PE=4 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.8e-184
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0


Query: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA 60
           MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA
Sbjct: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA 60

Query: 61  NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA 120
           NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA
Sbjct: 61  NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA 120

Query: 121 VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP 180
           VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP
Sbjct: 121 VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP 180

Query: 181 EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP 240
           EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP
Sbjct: 181 EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP 240

Query: 241 TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH 300
           TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH
Sbjct: 241 TLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAH 300

Query: 301 QSSQAKIHHQNDPKSE 317
           QSSQAKIHHQNDPKSE
Sbjct: 301 QSSQAKIHHQNDPKSE 316

[Close]

BLAST of Carg00701 vs. ExPASy TrEMBL
Match: A0A5A7SYZ3 (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002920 PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 3.9e-171
Identity = 292/314 (92.99%), Postives = 305/314 (97.13%), Query Frame = 0


Query: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62
           QCF YGSIMEAWYRSCFSRVGL+SATTDLGNGTVMHCWIPKT ++TKPNLVLIHGMGANA
Sbjct: 4   QCFSYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPKQTKPNLVLIHGMGANA 63

Query: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 122
           +WQWN+FVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG
Sbjct: 64  MWQWNQFVRPLITHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 123

Query: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182
           ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLED+DMEDGMFQVKSVEEAVSVLLPQ+PEK
Sbjct: 124 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQSPEK 183

Query: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242
           LKEMI+IAFFKPIRIGPSC+VNDLIDELCTEYREQKKELIQALHKERK S+LPKIT PTL
Sbjct: 184 LKEMIKIAFFKPIRIGPSCVVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITNPTL 243

Query: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302
           IMWGEKDLVFPMELAHRLK HIG+GAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA  S
Sbjct: 244 IMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 303

Query: 303 SQAKIHHQNDPKSE 317
           ++AKIHHQND KSE
Sbjct: 304 TKAKIHHQNDLKSE 317

[Close]

BLAST of Carg00701 vs. ExPASy TrEMBL
Match: A0A1S3B1G1 (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like OS=Cucumis melo OX=3656 GN=LOC103484964 PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 3.9e-171
Identity = 292/314 (92.99%), Postives = 305/314 (97.13%), Query Frame = 0


Query: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62
           QCF YGSIMEAWYRSCFSRVGL+SATTDLGNGTVMHCWIPKT ++TKPNLVLIHGMGANA
Sbjct: 4   QCFSYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPKQTKPNLVLIHGMGANA 63

Query: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 122
           +WQWN+FVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG
Sbjct: 64  MWQWNQFVRPLITHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 123

Query: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182
           ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLED+DMEDGMFQVKSVEEAVSVLLPQ+PEK
Sbjct: 124 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQSPEK 183

Query: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242
           LKEMI+IAFFKPIRIGPSC+VNDLIDELCTEYREQKKELIQALHKERK S+LPKIT PTL
Sbjct: 184 LKEMIKIAFFKPIRIGPSCVVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITNPTL 243

Query: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302
           IMWGEKDLVFPMELAHRLK HIG+GAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA  S
Sbjct: 244 IMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 303

Query: 303 SQAKIHHQNDPKSE 317
           ++AKIHHQND KSE
Sbjct: 304 TKAKIHHQNDLKSE 317

[Close]

BLAST of Carg00701 vs. ExPASy TrEMBL
Match: A0A0A0KLW0 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G499850 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.5e-170
Identity = 290/314 (92.36%), Postives = 305/314 (97.13%), Query Frame = 0


Query: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62
           QCF YGSIM+AWYRSCFSRVGL SATTDLGNGTVMHCWIPKT +ETKPNLVLIHGMGANA
Sbjct: 4   QCFSYGSIMDAWYRSCFSRVGLTSATTDLGNGTVMHCWIPKTPKETKPNLVLIHGMGANA 63

Query: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 122
           +WQWN+FVRPL++HFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG
Sbjct: 64  MWQWNQFVRPLVSHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 123

Query: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182
           +SYGGFVAYSMAAQFPDR+EKLVLCCTGVCLED+DMEDGMFQVKSVEEAVSVLLPQ+PEK
Sbjct: 124 VSYGGFVAYSMAAQFPDRVEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQSPEK 183

Query: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242
           LKEMI+IAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERK S+LPKIT PTL
Sbjct: 184 LKEMIKIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITNPTL 243

Query: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302
           IMWGEKDLVFPMELAHRLK HIG+GAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA  S
Sbjct: 244 IMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 303

Query: 303 SQAKIHHQNDPKSE 317
           ++AKIHHQND KSE
Sbjct: 304 TKAKIHHQNDLKSE 317

[Close]

BLAST of Carg00701 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 386.3 bits (991), Expect = 2.3e-107
Identity = 176/297 (59.26%), Postives = 233/297 (78.45%), Query Frame = 0


Query: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGA 60
           MA CF Y S     Y+  FSR GLRS+T+DLG+GTV HCWIP T   TKP L+L+HG+GA
Sbjct: 1   MASCFSYVSSRNKCYQYSFSRAGLRSSTSDLGDGTVFHCWIPLTHIHTKPTLLLLHGIGA 60

Query: 61  NAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNA 120
           NA+WQW++F+   I  FN+YVPDL+FFG+SYTT  DRSE+FQA CVM  +DA+GVRT   
Sbjct: 61  NAMWQWDRFIDRFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGVRTMTV 120

Query: 121 VGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTP 180
            G+SYGGFVAYS+AAQF +R++++VL C GV LE+KD EDGMF+VKS EEA +VL PQ+P
Sbjct: 121 AGLSYGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQSP 180

Query: 181 EKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQP 240
             L+ +++++F+KP    PSC   D I  +C +Y +++KEL++ALHK R+F++LPKITQP
Sbjct: 181 SMLRRLLQLSFYKPPIWIPSCFAMDYIHVMCKDYLQERKELVEALHKGRRFANLPKITQP 240

Query: 241 TLIMWGEKDLVFPMELAHRLKSHIG-DGAELVVIKKAGHALNIEKPKEMNKLIQCFL 297
           TL++WGE+D VFP+ELAHRLK ++G D A+LV++KK GHA+N EKPKEM K ++ FL
Sbjct: 241 TLMIWGEEDQVFPVELAHRLKRYLGEDRAQLVLLKKTGHAINEEKPKEMYKHMKSFL 297

[Close]

BLAST of Carg00701 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 378.6 bits (971), Expect = 4.8e-105
Identity = 171/304 (56.25%), Postives = 237/304 (77.96%), Query Frame = 0


Query: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNG-----TVMHCWIPKTRRETKPNLVLI 60
           + +CF + +  +  +R  F+  GLRS TTDL +G     T MHCWIPK+   +KPNL+L+
Sbjct: 7   LLRCFSFTASRDWLFRQSFANAGLRSVTTDLSHGNSIASTAMHCWIPKSPNRSKPNLLLL 66

Query: 61  HGMGANAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGV 120
           HG GANA+WQ+ + +R     FN+YVPDL+FFG S T+  +R+E+FQARC+M +++AHGV
Sbjct: 67  HGFGANAMWQYGEHLRAFTGRFNVYVPDLLFFGLSSTSEPNRTESFQARCLMRLMEAHGV 126

Query: 121 RTTNAVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVL 180
           +  N VGISYGGFV YS+AAQFP+ +EKLVLCC GVCLE+KDMEDG+F+V ++EEA  +L
Sbjct: 127 QRMNIVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPNLEEATGIL 186

Query: 181 LPQTPEKLKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLP 240
           +PQTPEKLKE+IR +F KPI+  PS  + D ID +CTE+ E+K++LI+++ K+R+ SDLP
Sbjct: 187 IPQTPEKLKELIRFSFVKPIKGVPSFFLWDFIDVMCTEFVEEKRDLIKSILKDRRLSDLP 246

Query: 241 KITQPTLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCF 300
           +I Q +LI+WGE+D +FP+EL +RLK HIG+ AE+VVIKKAGHA+N+EK KE  K ++ F
Sbjct: 247 RIKQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVVIKKAGHAVNLEKSKEFVKHLKSF 306

[Close]

BLAST of Carg00701 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.0e-86
Identity = 146/303 (48.18%), Postives = 216/303 (71.29%), Query Frame = 0


Query: 1   MAQCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRET--KPNLVLIHGM 60
           M  CF     +E  Y+S F R GLR  T DL +GTV++ W+ KT+ E+  KPNL+LIHG+
Sbjct: 1   MTGCFSLSEALERTYKSGFKRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIHGL 60

Query: 61  GANAVWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTT 120
           GA A+WQW    R L  +FN+Y+PDLVFFG S TT  +RS+ FQA+ +M  L+A  V+  
Sbjct: 61  GATAIWQWYDVARRLSRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKKF 120

Query: 121 NAVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQ 180
           + VG+SYGGFV Y MA+ + D +EK+V+CC  VC+E+KDM+ G+F+V  ++EA  +L+P+
Sbjct: 121 SLVGLSYGGFVGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPE 180

Query: 181 TPEKLKEMIRIAFFKP--IRIGPSCLVNDLIDELCT-EYREQKKELIQALHKERKFSDLP 240
           + +KL+E++   F+KP   R+ P+CL++D I+   T +  E+K+ELI+A+ K+R  S++P
Sbjct: 181 SVKKLRELMGYIFYKPALARLVPTCLLHDFIEHALTRDNMEEKRELIKAIPKDRIISEIP 240

Query: 241 KITQPTLIMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCF 299
           K+ QPTLI+WGE D VFP+E+  RL+ H+GD  +LV+IK+ GH  N EKPK+  KL++ F
Sbjct: 241 KLKQPTLIIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLKSF 300

[Close]

BLAST of Carg00701 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 204.1 bits (518), Expect = 1.6e-52
Identity = 113/293 (38.57%), Postives = 169/293 (57.68%), Query Frame = 0


Query: 11  MEAWYRSCFSRVGLRSATTDLGNGTVMHCWIP----KTRRETKPNLVLIHGMGANAVWQW 70
           +EA  R  FS  GL   T  + + T +  W P     +    KP+L+L+HG G +AVWQW
Sbjct: 12  VEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLLHGFGPSAVWQW 71

Query: 71  NKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVGISYG 130
           +  V+PL   F +YVPDLVFFG S ++  +RSE FQA C+  +++   V   + VG SYG
Sbjct: 72  SHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVERFSVVGTSYG 131

Query: 131 GFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEKLKEM 190
           GFVAY+MA  FP+++EK+VL  +GV L   D E  + + K       V+LP +   L+  
Sbjct: 132 GFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAK-CHRIKEVMLPASATDLRRF 191

Query: 191 IRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALH--KERKFSDLPKITQPTLIM 250
             +   K +   P  ++ND   ++ +E RE+K EL++ L   K+ K +++  I Q  +++
Sbjct: 192 SGMVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDK-TNVSPIQQDVMLI 251

Query: 251 WGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLV 298
           WGE+D VFP+++AH LK  +G  A L VI+K  H    EK KE N  +  FL+
Sbjct: 252 WGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFNGFVMSFLL 302

[Close]

BLAST of Carg00701 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 190.7 bits (483), Expect = 1.8e-48
Identity = 97/294 (32.99%), Postives = 173/294 (58.84%), Query Frame = 0


Query: 10  IMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIP----KTRRETKPNLVLIHGMGANAVWQ 69
           ++E + R C    GL S T  + + T +H W P        + +P ++L+HG G +++WQ
Sbjct: 12  LLEGYLRRCLRAAGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGFGPSSMWQ 71

Query: 70  WNKFVRPLI-AHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVGIS 129
           W + ++    + F +Y PDLVFFG+S ++ ++R+E FQA C+  ++   G+   N  G S
Sbjct: 72  WRRQMQAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGKYNVAGTS 131

Query: 130 YGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEKLK 189
           YGGFVAY MA  +P+++EK+V+  +G+ +   D E  + Q  + E    V+LP T  + +
Sbjct: 132 YGGFVAYHMAKMWPEKVEKVVIASSGINMRKCDGE-SLLQRSNCECIEKVMLPSTATEFR 191

Query: 190 EMIRIA-FFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFS-DLPKITQPTL 249
            ++ +A  ++ +R+ P  L ND+I+ L  + R++K EL++ +   R  + ++  ++Q  L
Sbjct: 192 TLMALASSWRLVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDSLSQEVL 251

Query: 250 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFL 297
           I+WG+KD +FP+++A+ LK  +GD  +L +I    H   IE  +E N ++  FL
Sbjct: 252 IVWGDKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFL 304

The following BLAST results are available for this feature:

BLAST of Carg00701 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950912.1	3.7e-184	100.00	uncharacterized protein LOC111453865 [Cucurbita moschata] >XP_022978531.1 unchar...	[more]
XP_023543119.1	1.4e-183	99.68	uncharacterized protein LOC111803081 [Cucurbita pepo subsp. pepo]	[more]
XP_008440587.1	8.0e-171	92.99	PREDICTED: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Cucumis mel...	[more]
XP_004143463.1	3.0e-170	92.36	uncharacterized protein LOC101208157 [Cucumis sativus] >KGN48741.1 hypothetical ...	[more]
XP_038882658.1	3.2e-167	91.08	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Benincasa hispida]	[more]

BLAST of Carg00701 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q52532	2.6e-15	27.34	2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Pseu...	[more]
Q13QH4	6.5e-14	26.36	2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Para...	[more]
P9WNH2	8.4e-14	26.05	Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ...	[more]
P9WNH3	8.4e-14	26.05	Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 ...	[more]
P0A573	8.4e-14	26.05	Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2...	[more]

BLAST of Carg00701 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IMX6	1.8e-184	100.00	uncharacterized protein LOC111478485 OS=Cucurbita maxima OX=3661 GN=LOC111478485...	[more]
A0A6J1GG71	1.8e-184	100.00	uncharacterized protein LOC111453865 OS=Cucurbita moschata OX=3662 GN=LOC1114538...	[more]
A0A5A7SYZ3	3.9e-171	92.99	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like OS=Cucumis melo var. ma...	[more]
A0A1S3B1G1	3.9e-171	92.99	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like OS=Cucumis melo OX=3656...	[more]
A0A0A0KLW0	1.5e-170	92.36	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G499...	[more]

BLAST of Carg00701 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39955.1	2.3e-107	59.26	alpha/beta-Hydrolases superfamily protein	[more]
AT5G09430.1	4.8e-105	56.25	alpha/beta-Hydrolases superfamily protein	[more]
AT1G78210.1	1.0e-86	48.18	alpha/beta-Hydrolases superfamily protein	[more]
AT5G21950.1	1.6e-52	38.57	alpha/beta-Hydrolases superfamily protein	[more]
AT4G33180.1	1.8e-48	32.99	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 207..227
None	No IPR available	PANTHER	PTHR43139:SF45	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 2..305
None	No IPR available	PANTHER	PTHR43139	SI:DKEY-122A22.2	coord: 2..305
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 76..91 score: 29.09 coord: 134..147 score: 41.38 coord: 120..133 score: 38.92 coord: 241..255 score: 41.84
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 50..152 e-value: 5.7E-16 score: 58.9
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 76..91 score: 26.42 coord: 120..133 score: 23.38 coord: 273..295 score: 31.82 coord: 134..147 score: 26.3 coord: 239..255 score: 30.21
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 19..310 e-value: 1.3E-48 score: 167.9
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 23..296

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00701-RA	Carg00701-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0031072	heat shock protein binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051082	unfolded protein binding
molecular_function	GO:0003824	catalytic activity