Carg00585 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00585
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	thiosulfate sulfurtransferase 18-like
Location	Carg_Chr03: 5708297 .. 5710253 (-)
RNA-Seq Expression	Carg00585
Synteny	Carg00585

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTTTCCATTGAGGAAGTGGAGTCACCTCCATCCCAACGCCTATTCCCACTTACTTCTCCAGCAGATGTGGCAAAAAACGACATGTCGTCTCAATGGATTGAATCTGTTTCAATTTCCGGCTTCAATTCAATCCCCTTATTTCATTATAAATAGAAACTTCCATACCGCTCATTCGCAGTCAAATTTCGCCGGCAGTTTCCGCCGCCGTTGTTATTCCGCCCACTTTTCCGCGACGGTTTCTGGTTTTCCAGACTTCAAAAACCGCCCACAGATCTCGAACCACCGGAATCGCACCGCTAATCATGGGCTCCCTCGATAGGTCTCTCGTCTCTTTTATTTAATTTATACATCCTTCCTTCTTTTTCTCTCATTATAATTCTCCAATGACGGCTAATTCCTTTCTCCTTCTTCCATTTGGATTCCTGCTCTGTTTTCTCGTCTCCGGCAGCTTCGAAGCCGGAATCGTCACCGTCGACGTCAAAACCGCCAAGAACCTCCTCCATTCCGGCTACGCTTTTCTCGACGTCAGGTTGAATTTGCTCGTTTTTTGCGTCAATTTTTTTCCGCATTTTAGTTGATTTTGTTAAAATTATCGGTAGGACTGTCGAGGAGTTCAAGGAAGGGCACGTAGCGACGGAGAAGATCGTCAATATTCCGTATCAATTCAATTCTCCAGACGGTTAATTCTTTTTATAATCGCTTCGGTATCCTTCTGTTCTTCCGTTTGAATTTTCCGGTGAAGTGACTGAACGCCGCGGCGATGGACTTTTCAGGTAGGGTGAAGAATGATCGGTTTCTGGAGGAGGTGTCGGCGGTCTTTAAGAAAGACGACCGCCTCGTCGTGGTAATTTCCCTTTTTATCCTTTTATTCACTAAATATTTATTAAATTATTACGTATTTATTCTATAAACGTTCAATTTAGCTAATTTAGCTAATTTAGCTTTAGGAACTATTAAATTATTATATGTAAAATTAAAATAAACTTAAACCTTTTATATTATAAAATAAAAAAGTGTCCAAAATATAATATTATTTTATTTTAAGTGTTTTGAATTTTAAAAATATCTAATAAGTTATTTAATTTTAAATTTCAATTATTAAAAAAAAAAACCCCTTTAACTTTTATGGATAAATTTTTAAAAGATTAGATATAAAATAAATAATTTAATAATTTATTTAATTACAGAATTTAAAATTTAGAATGATAATCGGTTGAATTTAAAATTATTTCAAATAATATATTTCGTTGGTAAAAATAAATTATGGAAACCAATGTTACGTATACAAAAGAGATTTTATAAATAATTGACATAATTAAATTTTATACATTATCGTCGAGTATTTTATATGATTCAAACTACGATTTTTTTCAAATAGGGTTGTCGAAGTGGGGTAAGATCTTTGCTCGCCATTGAAGACCTCCGAAATGATGTAAGTTATATTTTAATTTTTTTAATTATTATAGGTTTCGTTCAATTTTAACATTTAAATGTTTAAATAAATCGACGTAAATTCATTTAAAATTTCAAAAGAAAATGGAAATCAATTGATTTAATAATTAAAGTATACTAATTACTATTAATTATTTAATTCTTAATCATGCACAGAATAAAATTTAAAATTCCAATGGATTAAATTATAATTCTTTATAGGGGAAAAAATAAAATAATATTAAAAAAATCTGCAGGGCTTCAAGCATTTGACAGATCTGGGCGGAGGGCATCTGGCTTGGCTGGAGTATGGATTTCCTGTGGCTAAGCCAAAAGTGAAGACAACTGATCCAAAGAGTGTGGACCTTAAGGCTGACGAGCTTTGATCGTACAATAAATAAATAAATAAAAATAAATAAAACGCATCAATAATTTGTCTTCTGGTTGTAAAGTTTATTATTGAATAATTTTATTTTATCTGATAAGTTTTAAAATAATAATATATTTAAATCAGTTGATTT

mRNA sequence

ATGCTTTTTCCATTGAGGAAGTGGAGTCACCTCCATCCCAACGCCTATTCCCACTTACTTCTCCAGCAGATGTGGCAAAAAACGACATGTCGTCTCAATGGATTGAATCTGTTTCAATTTCCGGCTTCAATTCAATCCCCTTATTTCATTATAAATAGAAACTTCCATACCGCTCATTCGCAGTCAAATTTCGCCGGCAGTTTCCGCCGCCGTTGTTATTCCGCCCACTTTTCCGCGACGACTTCAAAAACCGCCCACAGATCTCGAACCACCGGAATCGCACCGCTAATCATGGGCTCCCTCGATAGCTTCGAAGCCGGAATCGTCACCGTCGACGTCAAAACCGCCAAGAACCTCCTCCATTCCGGCTACGCTTTTCTCGACGTCAGGACTGTCGAGGAGTTCAAGGAAGGGCACGTAGCGACGGAGAAGATCGTCAATATTCCGTATCAATTCAATTCTCCAGACGGTAGGGTGAAGAATGATCGGTTTCTGGAGGAGGTGTCGGCGGTCTTTAAGAAAGACGACCGCCTCGTCGTGGGTTGTCGAAGTGGGGTAAGATCTTTGCTCGCCATTGAAGACCTCCGAAATGATGGCTTCAAGCATTTGACAGATCTGGGCGGAGGGCATCTGGCTTGGCTGGAGTATGGATTTCCTGTGGCTAAGCCAAAAGTGAAGACAACTGATCCAAAGAGTGTGGACCTTAAGGCTGACGAGCTTTGATCGTACAATAAATAAATAAATAAAAATAAATAAAACGCATCAATAATTTGTCTTCTGGTTGTAAAGTTTATTATTGAATAATTTTATTTTATCTGATAAGTTTTAAAATAATAATATATTTAAATCAGTTGATTT

Coding sequence (CDS)

ATGCTTTTTCCATTGAGGAAGTGGAGTCACCTCCATCCCAACGCCTATTCCCACTTACTTCTCCAGCAGATGTGGCAAAAAACGACATGTCGTCTCAATGGATTGAATCTGTTTCAATTTCCGGCTTCAATTCAATCCCCTTATTTCATTATAAATAGAAACTTCCATACCGCTCATTCGCAGTCAAATTTCGCCGGCAGTTTCCGCCGCCGTTGTTATTCCGCCCACTTTTCCGCGACGACTTCAAAAACCGCCCACAGATCTCGAACCACCGGAATCGCACCGCTAATCATGGGCTCCCTCGATAGCTTCGAAGCCGGAATCGTCACCGTCGACGTCAAAACCGCCAAGAACCTCCTCCATTCCGGCTACGCTTTTCTCGACGTCAGGACTGTCGAGGAGTTCAAGGAAGGGCACGTAGCGACGGAGAAGATCGTCAATATTCCGTATCAATTCAATTCTCCAGACGGTAGGGTGAAGAATGATCGGTTTCTGGAGGAGGTGTCGGCGGTCTTTAAGAAAGACGACCGCCTCGTCGTGGGTTGTCGAAGTGGGGTAAGATCTTTGCTCGCCATTGAAGACCTCCGAAATGATGGCTTCAAGCATTTGACAGATCTGGGCGGAGGGCATCTGGCTTGGCTGGAGTATGGATTTCCTGTGGCTAAGCCAAAAGTGAAGACAACTGATCCAAAGAGTGTGGACCTTAAGGCTGACGAGCTTTGA

Protein sequence

MLFPLRKWSHLHPNAYSHLLLQQMWQKTTCRLNGLNLFQFPASIQSPYFIINRNFHTAHSQSNFAGSFRRRCYSAHFSATTSKTAHRSRTTGIAPLIMGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKPKVKTTDPKSVDLKADEL

Homology

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BLAST of Carg00585 vs. NCBI nr
Match: KAG7034086.1 (Thiosulfate sulfurtransferase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 490.7 bits (1262), Expect = 7.0e-135
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MLFPLRKWSHLHPNAYSHLLLQQMWQKTTCRLNGLNLFQFPASIQSPYFIINRNFHTAHS 60
           MLFPLRKWSHLHPNAYSHLLLQQMWQKTTCRLNGLNLFQFPASIQSPYFIINRNFHTAHS
Sbjct: 1   MLFPLRKWSHLHPNAYSHLLLQQMWQKTTCRLNGLNLFQFPASIQSPYFIINRNFHTAHS 60

Query: 61  QSNFAGSFRRRCYSAHFSATTSKTAHRSRTTGIAPLIMGSLDSFEAGIVTVDVKTAKNLL 120
           QSNFAGSFRRRCYSAHFSATTSKTAHRSRTTGIAPLIMGSLDSFEAGIVTVDVKTAKNLL
Sbjct: 61  QSNFAGSFRRRCYSAHFSATTSKTAHRSRTTGIAPLIMGSLDSFEAGIVTVDVKTAKNLL 120

Query: 121 HSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEEVSAVFKKDDRLVV 180
           HSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEEVSAVFKKDDRLVV
Sbjct: 121 HSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEEVSAVFKKDDRLVV 180

Query: 181 GCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKPKVKTTDPKSVDLKADEL 240
           GCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKPKVKTTDPKSVDLKADEL
Sbjct: 181 GCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKPKVKTTDPKSVDLKADEL 240

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BLAST of Carg00585 vs. NCBI nr
Match: XP_023545011.1 (thiosulfate sulfurtransferase 18-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.0 bits (811), Expect = 1.4e-82
Identity = 158/160 (98.75%), Postives = 159/160 (99.38%), Query Frame = 0


Query: 81  TSKTAHRSRTTGIAPLIMGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHV 140
           TSKTAHRSRTTGIAPLIMGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHV
Sbjct: 28  TSKTAHRSRTTGIAPLIMGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHV 87

Query: 141 ATEKIVNIPYQFNSPDGRVKNDRFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGF 200
           ATEKIVNIPYQFNSPDGRVKNDRFLE+VSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGF
Sbjct: 88  ATEKIVNIPYQFNSPDGRVKNDRFLEDVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGF 147

Query: 201 KHLTDLGGGHLAWLEYGFPVAKPKVKTTDPKSVDLKADEL 241
           KHLTDLGGGHLAWLEYGFPVAKPKVK TDPKSVDLKADEL
Sbjct: 148 KHLTDLGGGHLAWLEYGFPVAKPKVK-TDPKSVDLKADEL 186

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BLAST of Carg00585 vs. NCBI nr
Match: XP_022949669.1 (thiosulfate sulfurtransferase 18-like [Cucurbita moschata])

HSP 1 Score: 285.0 bits (728), Expect = 5.9e-73
Identity = 138/143 (96.50%), Postives = 141/143 (98.60%), Query Frame = 0


Query: 98  MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDG 157
           MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVA EKIVNIPYQFNSPDG
Sbjct: 1   MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVANEKIVNIPYQFNSPDG 60

Query: 158 RVKNDRFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYG 217
           RVKND+FLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGF+HLTDLGGGHLAWL YG
Sbjct: 61  RVKNDQFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFEHLTDLGGGHLAWLAYG 120

Query: 218 FPVAKPKVKTTDPKSVDLKADEL 241
           FPVAKPKVKTTDPKSVDL+ADEL
Sbjct: 121 FPVAKPKVKTTDPKSVDLQADEL 143

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BLAST of Carg00585 vs. NCBI nr
Match: XP_022977637.1 (thiosulfate sulfurtransferase 18-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 283.5 bits (724), Expect = 1.7e-72
Identity = 139/143 (97.20%), Postives = 139/143 (97.20%), Query Frame = 0


Query: 98  MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDG 157
           MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDG
Sbjct: 1   MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDG 60

Query: 158 RVKNDRFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYG 217
           RVKNDRFLEEVSAVFKKDDRLVV CRSGVRSLLAIEDL NDGFKHLTDLGGGHLAWLE G
Sbjct: 61  RVKNDRFLEEVSAVFKKDDRLVVSCRSGVRSLLAIEDLLNDGFKHLTDLGGGHLAWLENG 120

Query: 218 FPVAKPKVKTTDPKSVDLKADEL 241
           FPVAKPKVKT DPKSVDLKADEL
Sbjct: 121 FPVAKPKVKTADPKSVDLKADEL 143

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BLAST of Carg00585 vs. NCBI nr
Match: XP_022977636.1 (thiosulfate sulfurtransferase 18-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 273.9 bits (699), Expect = 1.4e-69
Identity = 134/138 (97.10%), Postives = 134/138 (97.10%), Query Frame = 0


Query: 103 SFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKND 162
           SFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKND
Sbjct: 22  SFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKND 81

Query: 163 RFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAK 222
           RFLEEVSAVFKKDDRLVV CRSGVRSLLAIEDL NDGFKHLTDLGGGHLAWLE GFPVAK
Sbjct: 82  RFLEEVSAVFKKDDRLVVSCRSGVRSLLAIEDLLNDGFKHLTDLGGGHLAWLENGFPVAK 141

Query: 223 PKVKTTDPKSVDLKADEL 241
           PKVKT DPKSVDLKADEL
Sbjct: 142 PKVKTADPKSVDLKADEL 159

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BLAST of Carg00585 vs. ExPASy Swiss-Prot
Match: Q9FKW8 (Thiosulfate sulfurtransferase 18 OS=Arabidopsis thaliana OX=3702 GN=STR18 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.9e-30
Identity = 59/113 (52.21%), Postives = 82/113 (72.57%), Query Frame = 0


Query: 108 IVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEE 167
           +V+VDV  AK LL SG+ +LDVRT +EF+ GH    KIVNIPY  N+P GRVKN  FLE+
Sbjct: 14  VVSVDVSQAKTLLQSGHQYLDVRTQDEFRRGHCEAAKIVNIPYMLNTPQGRVKNQEFLEQ 73

Query: 168 VSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPV 221
           VS++    D ++VGC+SG RSL A  +L   G+K + ++GGG+LAW+++ FP+
Sbjct: 74  VSSLLNPADDILVGCQSGARSLKATTELVAAGYKKVRNVGGGYLAWVDHSFPI 126

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BLAST of Carg00585 vs. ExPASy Swiss-Prot
Match: F4IPI4 (Rhodanese-like domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=STR17 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.1e-28
Identity = 64/136 (47.06%), Postives = 91/136 (66.91%), Query Frame = 0


Query: 105 EAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRF 164
           E  ++T+DV  A+ LL SGY FLDVRTVEEFK+GHV +E + N+PY   +P G+  N  F
Sbjct: 29  EPKVITIDVNQAQKLLDSGYTFLDVRTVEEFKKGHVDSENVFNVPYWLYTPQGQEINPNF 88

Query: 165 LEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKPK 224
           L+ VS++  + D L++GC+SGVRSL A + L + GFK + ++ GG++AW+   FPV   K
Sbjct: 89  LKHVSSLCNQTDHLILGCKSGVRSLHATKFLVSSGFKTVRNMDGGYIAWVNKRFPV---K 148

Query: 225 VKTTDPKSVDLKADEL 241
           V     +  +LK DEL
Sbjct: 149 V-----EHKELKYDEL 156

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BLAST of Carg00585 vs. ExPASy Swiss-Prot
Match: Q8RUD6 (Protein HIGH ARSENIC CONTENT 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HAC1 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 7.3e-26
Identity = 56/116 (48.28%), Postives = 79/116 (68.10%), Query Frame = 0


Query: 108 IVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEE 167
           + TVDV TAK  L +G+ +LDVRT EEF + HV  E+ +NIPY F + +GRV N  FL +
Sbjct: 41  VETVDVYTAKGFLSTGHRYLDVRTNEEFAKSHV--EEALNIPYMFKTDEGRVINPDFLSQ 100

Query: 168 VSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKP 224
           V++V KKD+ L+V C +G R   A  DL N+G+ H+ ++GGG+ AW++ GF   KP
Sbjct: 101 VASVCKKDEHLIVACNAGGRGSRACVDLLNEGYDHVANMGGGYSAWVDAGFAGDKP 154

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BLAST of Carg00585 vs. ExPASy Swiss-Prot
Match: Q38853 (Rhodanese-like domain-containing protein 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=STR15 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.9e-22
Identity = 48/111 (43.24%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 110 TVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEEVS 169
           +V V+ A+ L  +GY +LDVRT +EF  GH    + +N+PY +    G VKN  FL +VS
Sbjct: 72  SVPVRVARELAQAGYRYLDVRTPDEFSIGH--PTRAINVPYMYRVGSGMVKNPSFLRQVS 131

Query: 170 AVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPV 221
           + F+K D +++GC SG  S +A  DL   GF  +TD+ GG++AW E   PV
Sbjct: 132 SHFRKHDEIIIGCESGQMSFMASTDLLTAGFTAITDIAGGYVAWTENELPV 180

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BLAST of Carg00585 vs. ExPASy Swiss-Prot
Match: P27626 (Senescence-associated protein DIN1 OS=Raphanus sativus OX=3726 GN=DIN1 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.1e-21
Identity = 48/111 (43.24%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 110 TVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEEVS 169
           +V V+ A+ L  +GY  LDVRT +EF  GH    + +N+PY +    G VKN  FL +VS
Sbjct: 73  SVPVRVARELAQAGYKHLDVRTPDEFSIGH--PSRAINVPYMYRVGSGMVKNPSFLRQVS 132

Query: 170 AVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPV 221
           + F+K D +++GC SG RSL+A  +L   GF  +TD+ GG++ W E   PV
Sbjct: 133 SHFRKHDEIIIGCESGERSLMASTELLTAGFTGVTDIAGGYVPWTENELPV 181

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BLAST of Carg00585 vs. ExPASy TrEMBL
Match: A0A6J1GDI0 (thiosulfate sulfurtransferase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111452991 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.8e-73
Identity = 138/143 (96.50%), Postives = 141/143 (98.60%), Query Frame = 0


Query: 98  MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDG 157
           MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVA EKIVNIPYQFNSPDG
Sbjct: 1   MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVANEKIVNIPYQFNSPDG 60

Query: 158 RVKNDRFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYG 217
           RVKND+FLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGF+HLTDLGGGHLAWL YG
Sbjct: 61  RVKNDQFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFEHLTDLGGGHLAWLAYG 120

Query: 218 FPVAKPKVKTTDPKSVDLKADEL 241
           FPVAKPKVKTTDPKSVDL+ADEL
Sbjct: 121 FPVAKPKVKTTDPKSVDLQADEL 143

[Close]

BLAST of Carg00585 vs. ExPASy TrEMBL
Match: A0A6J1IJ25 (thiosulfate sulfurtransferase 18-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477892 PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 8.3e-73
Identity = 139/143 (97.20%), Postives = 139/143 (97.20%), Query Frame = 0


Query: 98  MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDG 157
           MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDG
Sbjct: 1   MGSLDSFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDG 60

Query: 158 RVKNDRFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYG 217
           RVKNDRFLEEVSAVFKKDDRLVV CRSGVRSLLAIEDL NDGFKHLTDLGGGHLAWLE G
Sbjct: 61  RVKNDRFLEEVSAVFKKDDRLVVSCRSGVRSLLAIEDLLNDGFKHLTDLGGGHLAWLENG 120

Query: 218 FPVAKPKVKTTDPKSVDLKADEL 241
           FPVAKPKVKT DPKSVDLKADEL
Sbjct: 121 FPVAKPKVKTADPKSVDLKADEL 143

[Close]

BLAST of Carg00585 vs. ExPASy TrEMBL
Match: A0A6J1IKH8 (thiosulfate sulfurtransferase 18-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477892 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 6.5e-70
Identity = 134/138 (97.10%), Postives = 134/138 (97.10%), Query Frame = 0


Query: 103 SFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKND 162
           SFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKND
Sbjct: 22  SFEAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKND 81

Query: 163 RFLEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAK 222
           RFLEEVSAVFKKDDRLVV CRSGVRSLLAIEDL NDGFKHLTDLGGGHLAWLE GFPVAK
Sbjct: 82  RFLEEVSAVFKKDDRLVVSCRSGVRSLLAIEDLLNDGFKHLTDLGGGHLAWLENGFPVAK 141

Query: 223 PKVKTTDPKSVDLKADEL 241
           PKVKT DPKSVDLKADEL
Sbjct: 142 PKVKTADPKSVDLKADEL 159

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BLAST of Carg00585 vs. ExPASy TrEMBL
Match: A0A0A0KJE7 (Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507040 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.7e-63
Identity = 147/245 (60.00%), Postives = 165/245 (67.35%), Query Frame = 0


Query: 1   MLFPLRKWSHLHPNAYSHLLLQQMWQKTTCRLNGLNLFQFPASIQ--SPYFIINRNFHTA 60
           ++  LRKWSHL PN        +   KTTCR         P + Q    Y  I RNF+  
Sbjct: 7   IILQLRKWSHLQPNPTPSFFSGRCC-KTTCR---------PTNQQRSESYLTIKRNFNVL 66

Query: 61  HSQSNFAGSFRRRCYSAHFSATTSKTAHRSRTTGIAPLIMGSLD-SFEAGIVTVDVKTAK 120
               N               + T KTAH  RT GI P IMGSLD S ++GIVTVDVKTA 
Sbjct: 67  LRHQNLPAVSAAVLLPP--QSPTLKTAHGFRTAGIRPPIMGSLDSSSDSGIVTVDVKTAD 126

Query: 121 NLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEEVSAVFKKDDR 180
           NLLHSGYAFLDVRTVEEFKEGH+ATE+IVNIPY  NSP+GRVKN +FL EVSAVFKKDDR
Sbjct: 127 NLLHSGYAFLDVRTVEEFKEGHMATERIVNIPYLLNSPNGRVKNAQFLAEVSAVFKKDDR 186

Query: 181 LVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKPKVK-TTDPKSV-DL 240
           LVVGCRSGVRSLLAIE+L+NDG+KHL DLGGGHLAWL+   PVA P  K T DPK + D 
Sbjct: 187 LVVGCRSGVRSLLAIEELQNDGYKHLKDLGGGHLAWLDNALPVANPIEKLTNDPKVIEDR 239

[Close]

BLAST of Carg00585 vs. ExPASy TrEMBL
Match: A0A5D3CKR3 (26S proteasome non-ATPase regulatory subunit 4-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001180 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 3.5e-63
Identity = 144/242 (59.50%), Postives = 162/242 (66.94%), Query Frame = 0


Query: 1   MLFPLRKWSHLHPNAYSHLLLQQMWQKTTCRLNGLNLFQFPASIQSPYFIINRNFHTAHS 60
           ++  LRKWSHL  N  +         KTTCR       +   +I+        NFH    
Sbjct: 7   IILRLRKWSHLQHNP-TPTFFSGRCCKTTCRPTNQERSESNLTIKG-------NFHVLLR 66

Query: 61  QSNFAGSFRRRCYSAHFSATTSKTAHRSRTTGIAPLIMGSLDSFEAGIVTVDVKTAKNLL 120
             N               + T KTAH  RT GI P IMGSLDS ++GIVTVDVKTA NLL
Sbjct: 67  HQNLPAVSAAVVLPP--QSPTLKTAHGFRTAGIRPPIMGSLDSSDSGIVTVDVKTADNLL 126

Query: 121 HSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEEVSAVFKKDDRLVV 180
           HSGYAFLDVRTVEEFKEGHVATE+IVNIPY  NSP+GR+KN +FL EVSAVFKKDDRLVV
Sbjct: 127 HSGYAFLDVRTVEEFKEGHVATERIVNIPYLLNSPNGRMKNAQFLAEVSAVFKKDDRLVV 186

Query: 181 GCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKPKVK-TTDPKSV-DLKAD 240
           GCRSGVRSLLAIE+L+N+G+KHL DLGGGHLAWLE   PVA P  K T DPK + D K D
Sbjct: 187 GCRSGVRSLLAIEELQNNGYKHLKDLGGGHLAWLENALPVANPNEKLTNDPKVIKDCKID 238

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BLAST of Carg00585 vs. TAIR 10
Match: AT5G66170.3 (sulfurtransferase 18 )

HSP 1 Score: 133.7 bits (335), Expect = 2.0e-31
Identity = 59/113 (52.21%), Postives = 82/113 (72.57%), Query Frame = 0


Query: 108 IVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEE 167
           +V+VDV  AK LL SG+ +LDVRT +EF+ GH    KIVNIPY  N+P GRVKN  FLE+
Sbjct: 14  VVSVDVSQAKTLLQSGHQYLDVRTQDEFRRGHCEAAKIVNIPYMLNTPQGRVKNQEFLEQ 73

Query: 168 VSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPV 221
           VS++    D ++VGC+SG RSL A  +L   G+K + ++GGG+LAW+++ FP+
Sbjct: 74  VSSLLNPADDILVGCQSGARSLKATTELVAAGYKKVRNVGGGYLAWVDHSFPI 126

[Close]

BLAST of Carg00585 vs. TAIR 10
Match: AT5G66170.2 (sulfurtransferase 18 )

HSP 1 Score: 129.8 bits (325), Expect = 2.9e-30
Identity = 59/115 (51.30%), Postives = 82/115 (71.30%), Query Frame = 0


Query: 108 IVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEE 167
           +V+VDV  AK LL SG+ +LDVRT +EF+ GH    KIVNIPY  N+P GRVKN  FLE+
Sbjct: 14  VVSVDVSQAKTLLQSGHQYLDVRTQDEFRRGHCEAAKIVNIPYMLNTPQGRVKNQEFLEQ 73

Query: 168 VSAVFKKDDRLVVGCRSGVRSLLAIEDL--RNDGFKHLTDLGGGHLAWLEYGFPV 221
           VS++    D ++VGC+SG RSL A  +L     G+K + ++GGG+LAW+++ FP+
Sbjct: 74  VSSLLNPADDILVGCQSGARSLKATTELVAAVSGYKKVRNVGGGYLAWVDHSFPI 128

[Close]

BLAST of Carg00585 vs. TAIR 10
Match: AT2G17850.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.5e-29
Identity = 64/136 (47.06%), Postives = 91/136 (66.91%), Query Frame = 0


Query: 105 EAGIVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRF 164
           E  ++T+DV  A+ LL SGY FLDVRTVEEFK+GHV +E + N+PY   +P G+  N  F
Sbjct: 29  EPKVITIDVNQAQKLLDSGYTFLDVRTVEEFKKGHVDSENVFNVPYWLYTPQGQEINPNF 88

Query: 165 LEEVSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKPK 224
           L+ VS++  + D L++GC+SGVRSL A + L + GFK + ++ GG++AW+   FPV   K
Sbjct: 89  LKHVSSLCNQTDHLILGCKSGVRSLHATKFLVSSGFKTVRNMDGGYIAWVNKRFPV---K 148

Query: 225 VKTTDPKSVDLKADEL 241
           V     +  +LK DEL
Sbjct: 149 V-----EHKELKYDEL 156

[Close]

BLAST of Carg00585 vs. TAIR 10
Match: AT2G21045.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 5.2e-27
Identity = 56/116 (48.28%), Postives = 79/116 (68.10%), Query Frame = 0


Query: 108 IVTVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEE 167
           + TVDV TAK  L +G+ +LDVRT EEF + HV  E+ +NIPY F + +GRV N  FL +
Sbjct: 41  VETVDVYTAKGFLSTGHRYLDVRTNEEFAKSHV--EEALNIPYMFKTDEGRVINPDFLSQ 100

Query: 168 VSAVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPVAKP 224
           V++V KKD+ L+V C +G R   A  DL N+G+ H+ ++GGG+ AW++ GF   KP
Sbjct: 101 VASVCKKDEHLIVACNAGGRGSRACVDLLNEGYDHVANMGGGYSAWVDAGFAGDKP 154

[Close]

BLAST of Carg00585 vs. TAIR 10
Match: AT4G35770.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 3.5e-23
Identity = 48/111 (43.24%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 110 TVDVKTAKNLLHSGYAFLDVRTVEEFKEGHVATEKIVNIPYQFNSPDGRVKNDRFLEEVS 169
           +V V+ A+ L  +GY +LDVRT +EF  GH    + +N+PY +    G VKN  FL +VS
Sbjct: 72  SVPVRVARELAQAGYRYLDVRTPDEFSIGH--PTRAINVPYMYRVGSGMVKNPSFLRQVS 131

Query: 170 AVFKKDDRLVVGCRSGVRSLLAIEDLRNDGFKHLTDLGGGHLAWLEYGFPV 221
           + F+K D +++GC SG  S +A  DL   GF  +TD+ GG++AW E   PV
Sbjct: 132 SHFRKHDEIIIGCESGQMSFMASTDLLTAGFTAITDIAGGYVAWTENELPV 180

The following BLAST results are available for this feature:

BLAST of Carg00585 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7034086.1	7.0e-135	100.00	Thiosulfate sulfurtransferase 18, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_023545011.1	1.4e-82	98.75	thiosulfate sulfurtransferase 18-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022949669.1	5.9e-73	96.50	thiosulfate sulfurtransferase 18-like [Cucurbita moschata]	[more]
XP_022977637.1	1.7e-72	97.20	thiosulfate sulfurtransferase 18-like isoform X2 [Cucurbita maxima]	[more]
XP_022977636.1	1.4e-69	97.10	thiosulfate sulfurtransferase 18-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg00585 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKW8	2.9e-30	52.21	Thiosulfate sulfurtransferase 18 OS=Arabidopsis thaliana OX=3702 GN=STR18 PE=1 S...	[more]
F4IPI4	2.1e-28	47.06	Rhodanese-like domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=S...	[more]
Q8RUD6	7.3e-26	48.28	Protein HIGH ARSENIC CONTENT 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q38853	4.9e-22	43.24	Rhodanese-like domain-containing protein 15, chloroplastic OS=Arabidopsis thalia...	[more]
P27626	1.1e-21	43.24	Senescence-associated protein DIN1 OS=Raphanus sativus OX=3726 GN=DIN1 PE=2 SV=1	[more]

BLAST of Carg00585 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GDI0	2.8e-73	96.50	thiosulfate sulfurtransferase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111452...	[more]
A0A6J1IJ25	8.3e-73	97.20	thiosulfate sulfurtransferase 18-like isoform X2 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1IKH8	6.5e-70	97.10	thiosulfate sulfurtransferase 18-like isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A0A0KJE7	2.7e-63	60.00	Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507040 P...	[more]
A0A5D3CKR3	3.5e-63	59.50	26S proteasome non-ATPase regulatory subunit 4-like protein OS=Cucumis melo var....	[more]

BLAST of Carg00585 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66170.3	2.0e-31	52.21	sulfurtransferase 18	[more]
AT5G66170.2	2.9e-30	51.30	sulfurtransferase 18	[more]
AT2G17850.1	1.5e-29	47.06	Rhodanese/Cell cycle control phosphatase superfamily protein	[more]
AT2G21045.1	5.2e-27	48.28	Rhodanese/Cell cycle control phosphatase superfamily protein	[more]
AT4G35770.1	3.5e-23	43.24	Rhodanese/Cell cycle control phosphatase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001763	Rhodanese-like domain	SMART	SM00450	rhod_4	coord: 111..219 e-value: 1.4E-11 score: 54.5
IPR001763	Rhodanese-like domain	PFAM	PF00581	Rhodanese	coord: 114..214 e-value: 1.0E-6 score: 29.2
IPR001763	Rhodanese-like domain	PROSITE	PS50206	RHODANESE_3	coord: 120..222 score: 15.142308
IPR036873	Rhodanese-like domain superfamily	GENE3D	3.40.250.10		coord: 102..221 e-value: 5.9E-28 score: 99.3
IPR036873	Rhodanese-like domain superfamily	SUPERFAMILY	52821	Rhodanese/Cell cycle control phosphatase	coord: 107..220
IPR044684	Rhodanese-like domain-containing protein STR17/STR18/HARC1-like	PANTHER	PTHR44542	THIOSULFATE SULFURTRANSFERASE 18	coord: 83..224
None	No IPR available	PANTHER	PTHR44542:SF12	THIOSULFATE SULFURTRANSFERASE 18	coord: 83..224
None	No IPR available	CDD	cd00158	RHOD	coord: 115..213 e-value: 8.03504E-16 score: 68.4802

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00585-RA	Carg00585-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0003824	catalytic activity