Carg00516 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00516
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionSEC14 cytosolic factor-like
LocationCarg_Chr03: 5386207 .. 5389828 (-)
RNA-Seq ExpressionCarg00516
SyntenyCarg00516
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGGTCTATATATACGTGTGTGTGTGAGAGAATTATATAGATAGTCCCGAGTTTCTCTAACCTATTACATTAATCTCAAACTCAAATGGTACATGATTCAAAGTCCCTAATTAACTCAAGAATCCAAGACTCACAAACCTTTGGCACTCCATCAAACATCTCTATGTAGTTTTCTCCGACATATTTGTGCGCCAAAGCTATCAAATTTCCTGGCTCTAGGCTCCAACAAGCAAGGGAATGGACATTTTCCCTGCACCTTTCCTATTCAAGAAGGTTCCCACGGTTTTGCTTTCCACAATTAACTGGCCACAGTGTTTGGGAATAAAAAGAACAAATGGAAGAAGAAAAGAACCCGAAATCAAATGGTGAAGGGCACATGCAAGGACCACTGGACGGCCGAGATTGTATGATTGTGATATATATTTTAGTGGAGAATACTGGAAAAGAATTACGGAAGAGAAAACTGGAAAGGGAAACAAACCAGAGCCAGCAACTTCGACACGAAGAACCCATTCAGAATGTAAGCAGCTCCTTTTTCCTCTTCTTTGCTTCATTTTTTAAGGTTCGTAGTGAGATTCCCACAAGACACTGATGACTTTTGCGTGAGCTTAACAGGCATTGAGAAGGACAGGGCCATAAAGAAGGGCGAGTCCGTGTGAGACCCCTTTGGTATTTAAACAGCAGCCTGGTCTCTAAAGGTTGTGACTTGCGAGATCTTAGGTTCACTTTCTTGTCCGGGCTCTTCTTGTATTCTAGCATCGTCATTCTCGTACTTGAGCTGGTTTTCAACCTTGAGTTTCAAGCCCTGCAGAGAGAAAAACAGAGAGAATTAGAGAAGCAGAAGCTTTGGCAGATTGTATTTAGCTTTGGTGCAATTTCTATTCTTCTTGAGAAGGACCAAATGGGTGTCAATCCTCAGGACGCGATCAAGAAGCTCAAAGTTCTGATGGATCAAGGTCAGATGATCAACTCTGTTTTCTTCAATTCCAGTTCTGAAAACTTCATTCCCTAATAACCATTTGTTCTATCTGTTTAATTTCGTCACCTGCAGTTGATCACTCATCGAAGAAATCCTTTCAGGTTCGCCAGCTTTCTTCTTCTTCTTCTATCCTCCATCTTCTGTATGCGACCTGCAAGTTCTAAGAGCTTAAAATGCTGTTAGTTCTGTTGGGTTGAAGCCCAAATGACCTCTTATGAAACTTTTTGTAAATGACATCGAATGATCTATTTGCAGAATGTTCATCAAGGATTCTTAATAGAAACGCTGGACCGTTTCCTAAGGGCACGAGACTACGATGTTGGCAAGGCCCATAAAATGGTTAGACGCATGTTTTTGGTTTAACTTCTGTAAACATCAAACTGTCATATGCATTACTAAGATATGCTGACAAACCATTCCTCTTTAATGTTGCAGCTGGTTGACTGTTTAAAATGGAGGGTGGAAAACCAGATTGACAAGATATTAAGGGTAAGTGTTTCAACGGCTCGTTTACTCGATGGCTCCCAATTCTTTTCTTGACAGCATTTTATTGTCTGCAGAAGCCAATATTTCCTGCTGATGTCTATAGATCAGTGAGAGATTCACAGCTCGTAGGACTTTCGGGCTACTCAAAAGAGGTCTGAGTTTTGTTAACCAATGTCTGTCTTGTTTAACCAGCCATCCCTGTAATCTTAGGATACCTTGTTCTAATGAGATTAGCATATAGCCAGCCGTTCTAACCTACAACTTGTTCTGTTTCACCTCAGGGCCTTCCCATATTTGCCATTGGTGTTGGGCTCAGCACATTAGACAAAGCACATGTAAGTATTCGCAGTTCAGTTCATCTTCCAAACGAATAATTGACCCTTTCGTGAATTTCGATGCCACAAACAGCAGTCTAGATTTTGAAAATATGTACTTCAAAAACCAGAGAGAAATTGAGAAGCTTGTCAAGTTTACTAACCGGTGTTTTGATCTCTTCACAGGTGCATGATTATGTGCAGTCGCACATACAAATCAATGAGTATCGAGATCGTGTTATTCTGGTGAGTTTCTGAATTCTGGGATTCAAAAGAAGCTTCTCTCCTAGTATCATTAACACGTACATTTTGCTGTAGCCTTCTGCATCAAAAAAGTATGGAAGGCCCATCACTACCTGCGTGAAGATTTTAGATATGACTGGTCTGAAGCTTTCGGCACTTGGCCACATAAAGGTATTCTCTTTTCCTACAATTGTCTCCAATAGATGTCAGTGCAAGTTAAATATAGCCATGTAAATCTGATTCCGTTTCTTGTGGTTCTGATTGGTCTAAATTAATGACAAATCCGCACAATAAGATCAGAAAGTTAAATTCTTTAGTTTGAATCCATTTATCACAAATCAAGTTCACACTGGATCGTGGGATTTTGAGGGAAGAAAAAGAAGAAAAAAGAGACGAAAAAGAAAAGATTCAACGCATATCATTTTGTTTATAAAATCTGTTGTCTGGAGGAATTTCTTTCTGACCATCATTTTAAAACAAGACCATAATGGTGAGGAACATATTCTCCATAATAAACTATAGTAAAGTGGAAAGAAAACTTATGATGTGTTTTGATACCTTAAATATGCAATTTATGACTAGCAAATCAAGGATTTCCCAAATCTCTAATGATCATGCAACCGCTAATGTCTCATGTTTCTGCAGTTCCTGACAATTTTATCAACAATTGATGATCTGAACTACCCAGAGAGAACAACTGCTTACTACATTGTAAATGTACCTTACGTCTTTTCATCCTGTTGGAAGGTAAACCATTGAAGAGACTGATCTTGGTTGATTTTGTACCTTGAGTGTTGAAATAATTTTTCCTTCAACACCTGTCTGATGTGTATAGTCATGTATTCTCTTTGATATTGCAGGTTGTCAAGCCTCTCTTACATGAAAGGACAAGGAAAAAGGTTCAAGTGTTACCAGGCTGCGGGAGAGATGAGCTGTTGAAGGTAAGGCCTGCATCATTTAAAGAATGAAAAAATGGCTGACTCTTATCAAACATAAAAGCTCTTTTTTGGCCTGCTTGAATATATTATAAATAAAATGGACCACTACATGTTTTTTTATACAAAAAGTTTTGACATTTCATTTGACAATGGAAGCTAGAATCAAGTTTTCCGAAACATTCCATAAAGAATAACATATCGAGTGTATAAAGCAAGGTACATATGCTATTGTTAGACAATTAAGAGTGGCAAGAGTAGTGTTAAACACTAGTGGGGGAAATACAATAATCAAATAACGGGATTCTCTACCATATGCAAGTGATGATGCTTTAATTGTTCTCTATTTCTTGTAGATAATGGATTATACCTCTCTTCCACATTTCTGTAAAAGAGGGAGCTCATTGTCCTCCCGATCATCGGCTCATCAAGGAGGCAATAATTGCTATTCCTCGGACCACCTTTTTCATCAACAGCTATACAGCTACATCAAGCAACAATCCATGATCAATGAACCTACTGAGCCCATTAGAAAAGGATCTTTTCATGTAACCCTTCAGGCATCAGCATCAAAAAGCAAAGGAGCAGCTAAAACTATTGAATCTGAGTTTCGGAAGTATGAGAATGGGATTTCTGATACACTGATTGAACTGGAAATAATGTAG

mRNA sequence

TTGGTCTATATATACGTGTGTGTGTGAGAGAATTATATAGATAGTCCCGAGTTTCTCTAACCTATTACATTAATCTCAAACTCAAATGGTACATGATTCAAAGTCCCTAATTAACTCAAGAATCCAAGACTCACAAACCTTTGGCACTCCATCAAACATCTCTATGTAGTTTTCTCCGACATATTTGTGCGCCAAAGCTATCAAATTTCCTGGCTCTAGGCTCCAACAAGCAAGGGAATGGACATTTTCCCTGCACCTTTCCTATTCAAGAAGGTTCCCACGGTTTTGCTTTCCACAATTAACTGGCCACAGTGTTTGGGAATAAAAAGAACAAATGGAAGAAGAAAAGAACCCGAAATCAAATGGTGAAGGGCACATGCAAGGACCACTGGACGGCCGAGATTGTATGATTGTGATATATATTTTAGTGGAGAATACTGGAAAAGAATTACGGAAGAGAAAACTGGAAAGGGAAACAAACCAGAGCCAGCAACTTCGACACGAAGAACCCATTCAGAATGTAAGCAGCTCCTTTTTCCTCTTCTTTGCTTCATTTTTTAAGGTTCGTAGTGAGATTCCCACAAGACACTGATGACTTTTGCGTGAGCTTAACAGGCATTGAGAAGGACAGGGCCATAAAGAAGGGCGAGTCCGTGTGAGACCCCTTTGGTATTTAAACAGCAGCCTGGTCTCTAAAGGTTGTGACTTGCGAGATCTTAGGTTCACTTTCTTGTCCGGGCTCTTCTTGTATTCTAGCATCGTCATTCTCGTACTTGAGCTGGTTTTCAACCTTGAGTTTCAAGCCCTGCAGAGAGAAAAACAGAGAGAATTAGAGAAGCAGAAGCTTTGGCAGATTGTATTTAGCTTTGGTGCAATTTCTATTCTTCTTGAGAAGGACCAAATGGGTGTCAATCCTCAGGACGCGATCAAGAAGCTCAAAGTTCTGATGGATCAAGTTGATCACTCATCGAAGAAATCCTTTCAGAATGTTCATCAAGGATTCTTAATAGAAACGCTGGACCGTTTCCTAAGGGCACGAGACTACGATGTTGGCAAGGCCCATAAAATGCTGGTTGACTGTTTAAAATGGAGGGTGGAAAACCAGATTGACAAGATATTAAGGAAGCCAATATTTCCTGCTGATGTCTATAGATCAGTGAGAGATTCACAGCTCGTAGGACTTTCGGGCTACTCAAAAGAGGGCCTTCCCATATTTGCCATTGGTGTTGGGCTCAGCACATTAGACAAAGCACATGTGCATGATTATGTGCAGTCGCACATACAAATCAATGAGTATCGAGATCGTGTTATTCTGCCTTCTGCATCAAAAAAGTATGGAAGGCCCATCACTACCTGCGTGAAGATTTTAGATATGACTGGTCTGAAGCTTTCGGCACTTGGCCACATAAAGTTCCTGACAATTTTATCAACAATTGATGATCTGAACTACCCAGAGAGAACAACTGCTTACTACATTGTAAATGTACCTTACGTCTTTTCATCCTGTTGGAAGGTTGTCAAGCCTCTCTTACATGAAAGGACAAGGAAAAAGGTTCAAGTGTTACCAGGCTGCGGGAGAGATGAGCTGTTGAAGATAATGGATTATACCTCTCTTCCACATTTCTGTAAAAGAGGGAGCTCATTGTCCTCCCGATCATCGGCTCATCAAGGAGGCAATAATTGCTATTCCTCGGACCACCTTTTTCATCAACAGCTATACAGCTACATCAAGCAACAATCCATGATCAATGAACCTACTGAGCCCATTAGAAAAGGATCTTTTCATGTAACCCTTCAGGCATCAGCATCAAAAAGCAAAGGAGCAGCTAAAACTATTGAATCTGAGTTTCGGAAGTATGAGAATGGGATTTCTGATACACTGATTGAACTGGAAATAATGTAG

Coding sequence (CDS)

ATGGGTGTCAATCCTCAGGACGCGATCAAGAAGCTCAAAGTTCTGATGGATCAAGTTGATCACTCATCGAAGAAATCCTTTCAGAATGTTCATCAAGGATTCTTAATAGAAACGCTGGACCGTTTCCTAAGGGCACGAGACTACGATGTTGGCAAGGCCCATAAAATGCTGGTTGACTGTTTAAAATGGAGGGTGGAAAACCAGATTGACAAGATATTAAGGAAGCCAATATTTCCTGCTGATGTCTATAGATCAGTGAGAGATTCACAGCTCGTAGGACTTTCGGGCTACTCAAAAGAGGGCCTTCCCATATTTGCCATTGGTGTTGGGCTCAGCACATTAGACAAAGCACATGTGCATGATTATGTGCAGTCGCACATACAAATCAATGAGTATCGAGATCGTGTTATTCTGCCTTCTGCATCAAAAAAGTATGGAAGGCCCATCACTACCTGCGTGAAGATTTTAGATATGACTGGTCTGAAGCTTTCGGCACTTGGCCACATAAAGTTCCTGACAATTTTATCAACAATTGATGATCTGAACTACCCAGAGAGAACAACTGCTTACTACATTGTAAATGTACCTTACGTCTTTTCATCCTGTTGGAAGGTTGTCAAGCCTCTCTTACATGAAAGGACAAGGAAAAAGGTTCAAGTGTTACCAGGCTGCGGGAGAGATGAGCTGTTGAAGATAATGGATTATACCTCTCTTCCACATTTCTGTAAAAGAGGGAGCTCATTGTCCTCCCGATCATCGGCTCATCAAGGAGGCAATAATTGCTATTCCTCGGACCACCTTTTTCATCAACAGCTATACAGCTACATCAAGCAACAATCCATGATCAATGAACCTACTGAGCCCATTAGAAAAGGATCTTTTCATGTAACCCTTCAGGCATCAGCATCAAAAAGCAAAGGAGCAGCTAAAACTATTGAATCTGAGTTTCGGAAGTATGAGAATGGGATTTCTGATACACTGATTGAACTGGAAATAATGTAG

Protein sequence

MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDCLKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVHDYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPHFCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQASASKSKGAAKTIESEFRKYENGISDTLIELEIM
Homology
BLAST of Carg00516 vs. NCBI nr
Match: KAG6603837.1 (Phosphatidylinositol/phosphatidylcholine transfer protein SFH9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 671.0 bits (1730), Expect = 5.3e-189
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC
Sbjct: 153 MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 212

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH
Sbjct: 213 LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 272

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
           DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD
Sbjct: 273 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 332

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH
Sbjct: 333 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 392

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA
Sbjct: 393 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 452

Query: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
           SASKSKGAAKTIESEFRKYENGISDTLIELEIM
Sbjct: 453 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 485

BLAST of Carg00516 vs. NCBI nr
Match: XP_022950475.1 (SEC14 cytosolic factor-like [Cucurbita moschata] >XP_023543079.1 SEC14 cytosolic factor-like [Cucurbita pepo subsp. pepo] >KAG7034018.1 Phosphatidylinositol/phosphatidylcholine transfer protein SFH9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 671.0 bits (1730), Expect = 5.3e-189
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC
Sbjct: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH
Sbjct: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
           DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD
Sbjct: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH
Sbjct: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA
Sbjct: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300

Query: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
           SASKSKGAAKTIESEFRKYENGISDTLIELEIM
Sbjct: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 333

BLAST of Carg00516 vs. NCBI nr
Match: XP_022977706.1 (SEC14 cytosolic factor-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 663.3 bits (1710), Expect = 1.1e-186
Identity = 327/333 (98.20%), Postives = 331/333 (99.40%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MGVNPQDAIKKLKVLMDQVDHS++KSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC
Sbjct: 1   MGVNPQDAIKKLKVLMDQVDHSTRKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDK HVH
Sbjct: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKEHVH 120

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
           DYVQSHIQINEYRDRVILPSASKKYGRPIT+CVKILDMTGLKLSALGHIKFLTILSTIDD
Sbjct: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITSCVKILDMTGLKLSALGHIKFLTILSTIDD 180

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH
Sbjct: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FCKRGSSLSSRSSAHQGGNNCYSSDH FHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA
Sbjct: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHFFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300

Query: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
           SASKSKGAAKT+ESEFRKYENGISDTLIELEIM
Sbjct: 301 SASKSKGAAKTVESEFRKYENGISDTLIELEIM 333

BLAST of Carg00516 vs. NCBI nr
Match: XP_022977705.1 (SEC14 cytosolic factor-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 327/357 (91.60%), Postives = 331/357 (92.72%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MGVNPQDAIKKLKVLMDQVDHS++KSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC
Sbjct: 1   MGVNPQDAIKKLKVLMDQVDHSTRKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60

Query: 61  LKWRVENQIDKILR------------------------KPIFPADVYRSVRDSQLVGLSG 120
           LKWRVENQIDKILR                        KPIFPADVYRSVRDSQLVGLSG
Sbjct: 61  LKWRVENQIDKILRVSVSTARLLRWLPILFLTAFYCLQKPIFPADVYRSVRDSQLVGLSG 120

Query: 121 YSKEGLPIFAIGVGLSTLDKAHVHDYVQSHIQINEYRDRVILPSASKKYGRPITTCVKIL 180
           YSKEGLPIFAIGVGLSTLDK HVHDYVQSHIQINEYRDRVILPSASKKYGRPIT+CVKIL
Sbjct: 121 YSKEGLPIFAIGVGLSTLDKEHVHDYVQSHIQINEYRDRVILPSASKKYGRPITSCVKIL 180

Query: 181 DMTGLKLSALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRK 240
           DMTGLKLSALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRK
Sbjct: 181 DMTGLKLSALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRK 240

Query: 241 KVQVLPGCGRDELLKIMDYTSLPHFCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYI 300
           KVQVLPGCGRDELLKIMDYTSLPHFCKRGSSLSSRSSAHQGGNNCYSSDH FHQQLYSYI
Sbjct: 241 KVQVLPGCGRDELLKIMDYTSLPHFCKRGSSLSSRSSAHQGGNNCYSSDHFFHQQLYSYI 300

Query: 301 KQQSMINEPTEPIRKGSFHVTLQASASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
           KQQSMINEPTEPIRKGSFHVTLQASASKSKGAAKT+ESEFRKYENGISDTLIELEIM
Sbjct: 301 KQQSMINEPTEPIRKGSFHVTLQASASKSKGAAKTVESEFRKYENGISDTLIELEIM 357

BLAST of Carg00516 vs. NCBI nr
Match: XP_038882914.1 (phosphatidylinositol/phosphatidylcholine transfer protein SFH11-like isoform X2 [Benincasa hispida])

HSP 1 Score: 608.2 bits (1567), Expect = 4.2e-170
Identity = 297/333 (89.19%), Postives = 314/333 (94.29%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MG+NPQ+AIKKLK L+DQVD   KKSFQNVHQGF+ ETLDRFL+ARDYDVGKAHKMLVDC
Sbjct: 1   MGINPQEAIKKLKALIDQVDQPMKKSFQNVHQGFITETLDRFLKARDYDVGKAHKMLVDC 60

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           LKWRVEN+IDK+LRKPIFPADVYR+VRDSQLVGLSGYSKEGLP+FAIGVGLS LDKA+V+
Sbjct: 61  LKWRVENEIDKVLRKPIFPADVYRAVRDSQLVGLSGYSKEGLPVFAIGVGLSALDKANVN 120

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
           DYVQSHIQINEYRDRVILPSASK+YGRPITTCVKILDMTGLKLSALGH K LTILSTIDD
Sbjct: 121 DYVQSHIQINEYRDRVILPSASKRYGRPITTCVKILDMTGLKLSALGHTKLLTILSTIDD 180

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPERTTAYYIVN PYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDY+SLPH
Sbjct: 181 LNYPERTTAYYIVNAPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYSSLPH 240

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FCKR SSLSSRSSA QGGNNCYS DHLFHQQLY+YIKQQS+INEP EPIRKGSF V LQ 
Sbjct: 241 FCKRESSLSSRSSARQGGNNCYSLDHLFHQQLYNYIKQQSLINEPVEPIRKGSFQVNLQV 300

Query: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
            ASKSKGAAKTIE+E RKY NG+SDTLIELEIM
Sbjct: 301 PASKSKGAAKTIETELRKYGNGLSDTLIELEIM 333

BLAST of Carg00516 vs. ExPASy Swiss-Prot
Match: P46250 (SEC14 cytosolic factor OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=SEC14 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.5e-18
Identity = 66/213 (30.99%), Postives = 111/213 (52.11%), Query Frame = 0

Query: 38  TLDRFLRARDYDVGKAHKMLVDCLKWRVENQIDKILR------KPI----FPADVYRSVR 97
           +L RFLRAR +D+ KA  M V C KWR +  ++ IL+      KPI    +P   +++ +
Sbjct: 57  SLLRFLRARKFDIQKAIDMFVACEKWREDFGVNTILKDFHYEEKPIVAKMYPTYYHKTDK 116

Query: 98  DSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVHDYVQSHIQINEYRDRVILPSASKKYGR 157
           D + V    Y +E   +  + +   T  +  + + V  +  + +YR    LP+ S+K G 
Sbjct: 117 DGRPV----YFEELGKVDLVKMLKITTQERMLKNLVWEYEAMCQYR----LPACSRKAGY 176

Query: 158 PITTCVKILDMTGLKL-SALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVV 217
            + T   +LD++G+ + SA   I ++   S I    YPER   +Y++N P+ FS+ +K+ 
Sbjct: 177 LVETSCTVLDLSGISVTSAYNVIGYVREASKIGQDYYPERMGKFYLINAPFGFSTAFKLF 236

Query: 218 KPLLHERTRKKVQVLPGCGRDELLKIMDYTSLP 240
           KP L   T  K+ +L    + ELLK +   +LP
Sbjct: 237 KPFLDPVTVSKIHILGYSYKKELLKQIPPQNLP 261

BLAST of Carg00516 vs. ExPASy Swiss-Prot
Match: Q75DK1 (SEC14 cytosolic factor OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 93.2 bits (230), Expect = 5.9e-18
Identity = 63/213 (29.58%), Postives = 106/213 (49.77%), Query Frame = 0

Query: 38  TLDRFLRARDYDVGKAHKMLVDCLKWRVENQIDKIL------RKPI----FPADVYRSVR 97
           TL RFLRAR +DV  A  M  +C KWR EN +D I        KP+    +P   +++ +
Sbjct: 55  TLLRFLRARKFDVAAARAMFENCEKWRKENGVDTIFEDFHYEEKPLVAKFYPQYYHKTDK 114

Query: 98  DSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVHDYVQSHIQINEYRDRVILPSASKKYGR 157
           D + V    Y +E   +    +   T  +  + + +  +   + YR    LP++S++   
Sbjct: 115 DGRPV----YIEELGAVNLTEMYKITTQERMLKNLIWEYESFSRYR----LPASSRQADC 174

Query: 158 PITTCVKILDMTGLKLSALGHI-KFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVV 217
            + T   ILD+ G+ +SA   +  ++   S I    YPER   +Y++N P+ FS+ +++ 
Sbjct: 175 LVETSCTILDLKGISISAAAQVLSYVREASNIGQNYYPERMGKFYMINAPFGFSAAFRLF 234

Query: 218 KPLLHERTRKKVQVLPGCGRDELLKIMDYTSLP 240
           KP L   T  K+ +L    + ELLK +   +LP
Sbjct: 235 KPFLDPVTVSKIFILGSSYQKELLKQIPAENLP 259

BLAST of Carg00516 vs. ExPASy Swiss-Prot
Match: P24280 (SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC14 PE=1 SV=3)

HSP 1 Score: 90.9 bits (224), Expect = 3.0e-17
Identity = 64/213 (30.05%), Postives = 108/213 (50.70%), Query Frame = 0

Query: 38  TLDRFLRARDYDVGKAHKMLVDCLKWRVENQIDKILR------KPI----FPADVYRSVR 97
           TL RFLRAR +DV  A +M  +C KWR +   D IL+      KP+    +P   +++ +
Sbjct: 57  TLLRFLRARKFDVQLAKEMFENCEKWRKDYGTDTILQDFHYDEKPLIAKFYPQYYHKTDK 116

Query: 98  DSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVHDYVQSHIQINEYRDRVILPSASKKYGR 157
           D + V    Y +E   +    +   T ++  + + V  +  + +YR    LP+ S+  G 
Sbjct: 117 DGRPV----YFEELGAVNLHEMNKVTSEERMLKNLVWEYESVVQYR----LPACSRAAGH 176

Query: 158 PITTCVKILDMTGLKL-SALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVV 217
            + T   I+D+ G+ + SA   + ++   S I    YPER   +YI+N P+ FS+ +++ 
Sbjct: 177 LVETSCTIMDLKGISISSAYSVMSYVREASYISQNYYPERMGKFYIINAPFGFSTAFRLF 236

Query: 218 KPLLHERTRKKVQVLPGCGRDELLKIMDYTSLP 240
           KP L   T  K+ +L    + ELLK +   +LP
Sbjct: 237 KPFLDPVTVSKIFILGSSYQKELLKQIPAENLP 261

BLAST of Carg00516 vs. ExPASy Swiss-Prot
Match: Q7PWB1 (Protein real-time OS=Anopheles gambiae OX=7165 GN=retm PE=3 SV=4)

HSP 1 Score: 89.7 bits (221), Expect = 6.6e-17
Identity = 66/216 (30.56%), Postives = 103/216 (47.69%), Query Frame = 0

Query: 37  ETLDRFLRARDYDVGKAHKMLVDCLKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSG 96
           +TL RFLRARD+ + KA  ML + L+WR E +ID IL +   PA V +        G   
Sbjct: 256 QTLLRFLRARDFSIDKATGMLQESLQWRKEQRIDSILGEYKTPAVVEKYFPG----GWHH 315

Query: 97  YSKEGLPIFAIGVGL----STLDKAHVHDYVQSHIQINEYRDRVILPSASKKYGRPITTC 156
           + K+G P++ + +G       L      + ++  + I E   R ++  A+K +G+P+   
Sbjct: 316 HDKDGRPLYILRLGTMDVKGLLKSVGEDELLKLTLHICEEGLR-LMKEATKLFGKPVWNW 375

Query: 157 VKILDMTGLKLSAL---GHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPL 216
             ++D+ GL +  L   G    L I+ T+ + NYPE      IV  P VF   W +V   
Sbjct: 376 CLLVDLDGLSMRHLWRPGVKALLRIIETV-ETNYPETMGRVLIVRAPRVFPVLWTIVSTF 435

Query: 217 LHERTRKKVQVL--PGC--GRDELLKIMDYTSLPHF 242
           + E TR K      P C    D + + +D   +P F
Sbjct: 436 IDENTRSKFLFFGGPDCMHAEDGIEQYIDTDKIPSF 465

BLAST of Carg00516 vs. ExPASy Swiss-Prot
Match: Q92503 (SEC14-like protein 1 OS=Homo sapiens OX=9606 GN=SEC14L1 PE=1 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 6.6e-17
Identity = 66/228 (28.95%), Postives = 111/228 (48.68%), Query Frame = 0

Query: 28  QNVHQGFL--IETLDRFLRARDYDVGKAHKMLVDCLKWRVENQIDKILRKPIFPADVYRS 87
           Q  H+G +   E + RFLRARD+++ KA +++   L WR ++Q+D IL     P    + 
Sbjct: 267 QETHKGKIPKDEHILRFLRARDFNIDKAREIMCQSLTWRKQHQVDYILETWTPP----QV 326

Query: 88  VRDSQLVGLSGYSKEGLPIFAIGVG-------LSTLDKAHVHDYVQSHIQINEYRDRVIL 147
           ++D    G   + K+G P++ + +G       +  L +  +  YV   + INE   R   
Sbjct: 327 LQDYYAGGWHHHDKDGRPLYVLRLGQMDTKGLVRALGEEALLRYV---LSINEEGLR-RC 386

Query: 148 PSASKKYGRPITTCVKILDMTGLKLSALGH--IKFLTILSTIDDLNYPERTTAYYIVNVP 207
              +K +GRPI++   ++D+ GL +  L    +K L  +  + + NYPE      I+  P
Sbjct: 387 EENTKVFGRPISSWTCLVDLEGLNMRHLWRPGVKALLRIIEVVEANYPETLGRLLILRAP 446

Query: 208 YVFSSCWKVVKPLLHERTRKKVQVLPG---CGRDELLKIMDYTSLPHF 242
            VF   W +V P + + TR+K  +  G    G   LL  +D   +P F
Sbjct: 447 RVFPVLWTLVSPFIDDNTRRKFLIYAGNDYQGPGGLLDYIDKEIIPDF 486

BLAST of Carg00516 vs. ExPASy TrEMBL
Match: A0A6J1GFU6 (SEC14 cytosolic factor-like OS=Cucurbita moschata OX=3662 GN=LOC111453570 PE=4 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 2.6e-189
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC
Sbjct: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH
Sbjct: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
           DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD
Sbjct: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH
Sbjct: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA
Sbjct: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300

Query: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
           SASKSKGAAKTIESEFRKYENGISDTLIELEIM
Sbjct: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 333

BLAST of Carg00516 vs. ExPASy TrEMBL
Match: A0A6J1IKR1 (SEC14 cytosolic factor-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477929 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 5.3e-187
Identity = 327/333 (98.20%), Postives = 331/333 (99.40%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MGVNPQDAIKKLKVLMDQVDHS++KSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC
Sbjct: 1   MGVNPQDAIKKLKVLMDQVDHSTRKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDK HVH
Sbjct: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKEHVH 120

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
           DYVQSHIQINEYRDRVILPSASKKYGRPIT+CVKILDMTGLKLSALGHIKFLTILSTIDD
Sbjct: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITSCVKILDMTGLKLSALGHIKFLTILSTIDD 180

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH
Sbjct: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FCKRGSSLSSRSSAHQGGNNCYSSDH FHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA
Sbjct: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHFFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300

Query: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
           SASKSKGAAKT+ESEFRKYENGISDTLIELEIM
Sbjct: 301 SASKSKGAAKTVESEFRKYENGISDTLIELEIM 333

BLAST of Carg00516 vs. ExPASy TrEMBL
Match: A0A6J1IN41 (SEC14 cytosolic factor-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477929 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.1e-183
Identity = 327/357 (91.60%), Postives = 331/357 (92.72%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MGVNPQDAIKKLKVLMDQVDHS++KSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC
Sbjct: 1   MGVNPQDAIKKLKVLMDQVDHSTRKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60

Query: 61  LKWRVENQIDKILR------------------------KPIFPADVYRSVRDSQLVGLSG 120
           LKWRVENQIDKILR                        KPIFPADVYRSVRDSQLVGLSG
Sbjct: 61  LKWRVENQIDKILRVSVSTARLLRWLPILFLTAFYCLQKPIFPADVYRSVRDSQLVGLSG 120

Query: 121 YSKEGLPIFAIGVGLSTLDKAHVHDYVQSHIQINEYRDRVILPSASKKYGRPITTCVKIL 180
           YSKEGLPIFAIGVGLSTLDK HVHDYVQSHIQINEYRDRVILPSASKKYGRPIT+CVKIL
Sbjct: 121 YSKEGLPIFAIGVGLSTLDKEHVHDYVQSHIQINEYRDRVILPSASKKYGRPITSCVKIL 180

Query: 181 DMTGLKLSALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRK 240
           DMTGLKLSALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRK
Sbjct: 181 DMTGLKLSALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRK 240

Query: 241 KVQVLPGCGRDELLKIMDYTSLPHFCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYI 300
           KVQVLPGCGRDELLKIMDYTSLPHFCKRGSSLSSRSSAHQGGNNCYSSDH FHQQLYSYI
Sbjct: 241 KVQVLPGCGRDELLKIMDYTSLPHFCKRGSSLSSRSSAHQGGNNCYSSDHFFHQQLYSYI 300

Query: 301 KQQSMINEPTEPIRKGSFHVTLQASASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
           KQQSMINEPTEPIRKGSFHVTLQASASKSKGAAKT+ESEFRKYENGISDTLIELEIM
Sbjct: 301 KQQSMINEPTEPIRKGSFHVTLQASASKSKGAAKTVESEFRKYENGISDTLIELEIM 357

BLAST of Carg00516 vs. ExPASy TrEMBL
Match: A0A0A0KMW1 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510980 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.2e-166
Identity = 290/333 (87.09%), Postives = 308/333 (92.49%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MG NPQ+AIKKLK LMDQVD + KKSFQNVHQGF+ ET+DRFL+AR+YDV KAHKMLVDC
Sbjct: 1   MGNNPQEAIKKLKALMDQVDQAMKKSFQNVHQGFITETIDRFLKAREYDVAKAHKMLVDC 60

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           LKWRVEN+ID +LRKPI PADVYR+VRDSQLVGLSGYSKEGLP+FAIGVGLS LDKA V+
Sbjct: 61  LKWRVENEIDNVLRKPILPADVYRAVRDSQLVGLSGYSKEGLPVFAIGVGLSALDKATVN 120

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
           DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGH K LTILSTIDD
Sbjct: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHTKLLTILSTIDD 180

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPERTTAYYIVN PYVFSSCWKV+KPLLHERTRKKVQVLPGCG+DELLKIMDYTSLPH
Sbjct: 181 LNYPERTTAYYIVNAPYVFSSCWKVIKPLLHERTRKKVQVLPGCGKDELLKIMDYTSLPH 240

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FCKR SSLSSRSSA QGGNNCYS DH FHQQLY+YIKQQS+INEP EPIRKGSF V LQ 
Sbjct: 241 FCKRESSLSSRSSARQGGNNCYSLDHFFHQQLYNYIKQQSLINEPVEPIRKGSFQVNLQV 300

Query: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
            ASKSKGAA+TIE+E RKY N +SDTLIELEIM
Sbjct: 301 PASKSKGAARTIETELRKYGNRLSDTLIELEIM 333

BLAST of Carg00516 vs. ExPASy TrEMBL
Match: A0A5A7SKD0 (SEC14 cytosolic factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00330 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.6e-165
Identity = 288/333 (86.49%), Postives = 308/333 (92.49%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MG+NPQ+AIKKL+ LMD+VD   KKSFQNVHQGF+ ETL+RFL+AR+YDV KAHKMLVDC
Sbjct: 1   MGINPQEAIKKLRALMDRVDQPMKKSFQNVHQGFITETLERFLKAREYDVAKAHKMLVDC 60

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           LKWRVEN+ID +LRKPI PADVYR+VRDSQLVGLSGYSKEGLP+FAIGVGLS LDKA V+
Sbjct: 61  LKWRVENEIDNVLRKPILPADVYRAVRDSQLVGLSGYSKEGLPVFAIGVGLSALDKATVN 120

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
           DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGH K LTILSTIDD
Sbjct: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHTKLLTILSTIDD 180

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPERTTAYYIVN PYVFSSCWKV+KPLLHERTRKKVQVLPGCG++ELLKIMDYTSLPH
Sbjct: 181 LNYPERTTAYYIVNAPYVFSSCWKVIKPLLHERTRKKVQVLPGCGKEELLKIMDYTSLPH 240

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FCKR SSLSSRSSA QGGNNCYS DHLFHQQLY+YIKQQS+INEP EPIRKGSF V LQ 
Sbjct: 241 FCKRESSLSSRSSARQGGNNCYSLDHLFHQQLYNYIKQQSLINEPVEPIRKGSFQVNLQV 300

Query: 301 SASKSKGAAKTIESEFRKYENGISDTLIELEIM 334
            ASKSKG AKTIE+E RKY N +SDTLIELEIM
Sbjct: 301 PASKSKGTAKTIETELRKYGNRLSDTLIELEIM 333

BLAST of Carg00516 vs. TAIR 10
Match: AT5G47730.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 438.3 bits (1126), Expect = 5.4e-123
Identity = 212/321 (66.04%), Postives = 254/321 (79.13%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           MG+  ++AI + + LMDQV+   KK+++ VHQG+L E L RFL+ARD++V KAH MLV+C
Sbjct: 1   MGIVSEEAIDEFQELMDQVEEPLKKTYERVHQGYLRENLGRFLKARDWNVCKAHTMLVEC 60

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           L+WRV+N+ID IL KPI P ++YR VRDSQL+G+SGY+KEGLP+FAIGVGLST DKA VH
Sbjct: 61  LRWRVDNEIDSILSKPIVPTELYRDVRDSQLIGMSGYTKEGLPVFAIGVGLSTFDKASVH 120

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
            YVQSHIQINEYRDRV+LPS SKK GRPITTCVK+LDMTGLKLSAL  IK +TI+STIDD
Sbjct: 121 YYVQSHIQINEYRDRVLLPSISKKNGRPITTCVKVLDMTGLKLSALSQIKLVTIISTIDD 180

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPE+T  YY+VN PY+FS+CWKVVKPLL ERTRKKV VL GCGRDELLKIMD+TSLPH
Sbjct: 181 LNYPEKTNTYYVVNAPYIFSACWKVVKPLLQERTRKKVHVLSGCGRDELLKIMDFTSLPH 240

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FC+ G   SS SS H    NC+S +H FHQQLY+Y+K         EP ++GSFHV    
Sbjct: 241 FCRSG---SSGSSHHTQSANCFSINHPFHQQLYNYVKHHYETQGQAEPAKQGSFHVGFPE 300

Query: 301 SASKSKGAAKTIESEFRKYEN 322
             ++    AKTIESE  K+EN
Sbjct: 301 PEAERCVIAKTIESELHKFEN 318

BLAST of Carg00516 vs. TAIR 10
Match: AT1G55840.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 425.6 bits (1093), Expect = 3.6e-119
Identity = 199/321 (61.99%), Postives = 253/321 (78.82%), Query Frame = 0

Query: 1   MGVNPQDAIKKLKVLMDQVDHSSKKSFQNVHQGFLIETLDRFLRARDYDVGKAHKMLVDC 60
           M +  ++A+K+L+ LM+ VD S ++S++N+HQG+  E L RFL+ARD +V KAHKML++C
Sbjct: 1   MSITNEEAVKQLRALMEDVDDSLRESYRNIHQGYPTENLLRFLKARDGNVQKAHKMLLEC 60

Query: 61  LKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVH 120
           L+WR +N+IDKIL KPI P D+YR +RD+QLVG+SGYSKEGLP+ AIGVGLST DKA VH
Sbjct: 61  LEWRTQNEIDKILTKPIVPVDLYRGIRDTQLVGVSGYSKEGLPVIAIGVGLSTYDKASVH 120

Query: 121 DYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTILSTIDD 180
            YVQSHIQ+NEYRDRV+LPSASKK GRPI TC+KILDM+GLKLSAL  IK +T ++TIDD
Sbjct: 121 YYVQSHIQMNEYRDRVVLPSASKKQGRPICTCLKILDMSGLKLSALSQIKLMTAITTIDD 180

Query: 181 LNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDYTSLPH 240
           LNYPE+T  YY+VNVPY+FS+CWK +KPLL ERT+KK+QVL GCG+DELLKIMDY SLPH
Sbjct: 181 LNYPEKTETYYVVNVPYIFSACWKTIKPLLQERTKKKIQVLKGCGKDELLKIMDYESLPH 240

Query: 241 FCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFHVTLQA 300
           FC+R  S S R  ++   +NC+S DH FHQ LY Y+KQQ+++     PIR GS HV    
Sbjct: 241 FCRREGSGSGRHISNGTVDNCFSLDHSFHQDLYDYVKQQALVKGSGAPIRHGSVHVKFPE 300

Query: 301 SASKSKGAAKTIESEFRKYEN 322
             ++      T+E+EF+K  N
Sbjct: 301 PDTEGNKIFDTLENEFQKLGN 321

BLAST of Carg00516 vs. TAIR 10
Match: AT1G55840.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 4.3e-112
Identity = 189/293 (64.51%), Postives = 232/293 (79.18%), Query Frame = 0

Query: 29  NVHQGFLIETLDRFLRARDYDVGKAHKMLVDCLKWRVENQIDKILRKPIFPADVYRSVRD 88
           N+HQG+  E L RFL+ARD +V KAHKML++CL+WR +N+IDKIL KPI P D+YR +RD
Sbjct: 2   NIHQGYPTENLLRFLKARDGNVQKAHKMLLECLEWRTQNEIDKILTKPIVPVDLYRGIRD 61

Query: 89  SQLVGLSGYSKEGLPIFAIGVGLSTLDKAHVHDYVQSHIQINEYRDRVILPSASKKYGRP 148
           +QLVG+SGYSKEGLP+ AIGVGLST DKA VH YVQSHIQ+NEYRDRV+LPSASKK GRP
Sbjct: 62  TQLVGVSGYSKEGLPVIAIGVGLSTYDKASVHYYVQSHIQMNEYRDRVVLPSASKKQGRP 121

Query: 149 ITTCVKILDMTGLKLSALGHIKFLTILSTIDDLNYPERTTAYYIVNVPYVFSSCWKVVKP 208
           I TC+KILDM+GLKLSAL  IK +T ++TIDDLNYPE+T  YY+VNVPY+FS+CWK +KP
Sbjct: 122 ICTCLKILDMSGLKLSALSQIKLMTAITTIDDLNYPEKTETYYVVNVPYIFSACWKTIKP 181

Query: 209 LLHERTRKKVQVLPGCGRDELLKIMDYTSLPHFCKRGSSLSSRSSAHQGGNNCYSSDHLF 268
           LL ERT+KK+QVL GCG+DELLKIMDY SLPHFC+R  S S R  ++   +NC+S DH F
Sbjct: 182 LLQERTKKKIQVLKGCGKDELLKIMDYESLPHFCRREGSGSGRHISNGTVDNCFSLDHSF 241

Query: 269 HQQLYSYIKQQSMINEPTEPIRKGSFHVTLQASASKSKGAAKTIESEFRKYEN 322
           HQ LY Y+KQQ+++     PIR GS HV      ++      T+E+EF+K  N
Sbjct: 242 HQDLYDYVKQQALVKGSGAPIRHGSVHVKFPEPDTEGNKIFDTLENEFQKLGN 294

BLAST of Carg00516 vs. TAIR 10
Match: AT5G47730.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 381.3 bits (978), Expect = 7.8e-106
Identity = 184/266 (69.17%), Postives = 213/266 (80.08%), Query Frame = 0

Query: 56  MLVDCLKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLD 115
           MLV+CL+WRV+N+ID IL KPI P ++YR VRDSQL+G+SGY+KEGLP+FAIGVGLST D
Sbjct: 1   MLVECLRWRVDNEIDSILSKPIVPTELYRDVRDSQLIGMSGYTKEGLPVFAIGVGLSTFD 60

Query: 116 KAHVHDYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTIL 175
           KA VH YVQSHIQINEYRDRV+LPS SKK GRPITTCVK+LDMTGLKLSAL  IK +TI+
Sbjct: 61  KASVHYYVQSHIQINEYRDRVLLPSISKKNGRPITTCVKVLDMTGLKLSALSQIKLVTII 120

Query: 176 STIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDY 235
           STIDDLNYPE+T  YY+VN PY+FS+CWKVVKPLL ERTRKKV VL GCGRDELLKIMD+
Sbjct: 121 STIDDLNYPEKTNTYYVVNAPYIFSACWKVVKPLLQERTRKKVHVLSGCGRDELLKIMDF 180

Query: 236 TSLPHFCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFH 295
           TSLPHFC+ G   SS SS H    NC+S +H FHQQLY+Y+K         EP ++GSFH
Sbjct: 181 TSLPHFCRSG---SSGSSHHTQSANCFSINHPFHQQLYNYVKHHYETQGQAEPAKQGSFH 240

Query: 296 VTLQASASKSKGAAKTIESEFRKYEN 322
           V      ++    AKTIESE  K+EN
Sbjct: 241 VGFPEPEAERCVIAKTIESELHKFEN 263

BLAST of Carg00516 vs. TAIR 10
Match: AT5G47730.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 381.3 bits (978), Expect = 7.8e-106
Identity = 184/266 (69.17%), Postives = 213/266 (80.08%), Query Frame = 0

Query: 56  MLVDCLKWRVENQIDKILRKPIFPADVYRSVRDSQLVGLSGYSKEGLPIFAIGVGLSTLD 115
           MLV+CL+WRV+N+ID IL KPI P ++YR VRDSQL+G+SGY+KEGLP+FAIGVGLST D
Sbjct: 1   MLVECLRWRVDNEIDSILSKPIVPTELYRDVRDSQLIGMSGYTKEGLPVFAIGVGLSTFD 60

Query: 116 KAHVHDYVQSHIQINEYRDRVILPSASKKYGRPITTCVKILDMTGLKLSALGHIKFLTIL 175
           KA VH YVQSHIQINEYRDRV+LPS SKK GRPITTCVK+LDMTGLKLSAL  IK +TI+
Sbjct: 61  KASVHYYVQSHIQINEYRDRVLLPSISKKNGRPITTCVKVLDMTGLKLSALSQIKLVTII 120

Query: 176 STIDDLNYPERTTAYYIVNVPYVFSSCWKVVKPLLHERTRKKVQVLPGCGRDELLKIMDY 235
           STIDDLNYPE+T  YY+VN PY+FS+CWKVVKPLL ERTRKKV VL GCGRDELLKIMD+
Sbjct: 121 STIDDLNYPEKTNTYYVVNAPYIFSACWKVVKPLLQERTRKKVHVLSGCGRDELLKIMDF 180

Query: 236 TSLPHFCKRGSSLSSRSSAHQGGNNCYSSDHLFHQQLYSYIKQQSMINEPTEPIRKGSFH 295
           TSLPHFC+ G   SS SS H    NC+S +H FHQQLY+Y+K         EP ++GSFH
Sbjct: 181 TSLPHFCRSG---SSGSSHHTQSANCFSINHPFHQQLYNYVKHHYETQGQAEPAKQGSFH 240

Query: 296 VTLQASASKSKGAAKTIESEFRKYEN 322
           V      ++    AKTIESE  K+EN
Sbjct: 241 VGFPEPEAERCVIAKTIESELHKFEN 263

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6603837.15.3e-189100.00Phosphatidylinositol/phosphatidylcholine transfer protein SFH9, partial [Cucurbi... [more]
XP_022950475.15.3e-189100.00SEC14 cytosolic factor-like [Cucurbita moschata] >XP_023543079.1 SEC14 cytosolic... [more]
XP_022977706.11.1e-18698.20SEC14 cytosolic factor-like isoform X2 [Cucurbita maxima][more]
XP_022977705.11.3e-18291.60SEC14 cytosolic factor-like isoform X1 [Cucurbita maxima][more]
XP_038882914.14.2e-17089.19phosphatidylinositol/phosphatidylcholine transfer protein SFH11-like isoform X2 ... [more]
Match NameE-valueIdentityDescription
P462503.5e-1830.99SEC14 cytosolic factor OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=23... [more]
Q75DK15.9e-1829.58SEC14 cytosolic factor OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC... [more]
P242803.0e-1730.05SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ... [more]
Q7PWB16.6e-1730.56Protein real-time OS=Anopheles gambiae OX=7165 GN=retm PE=3 SV=4[more]
Q925036.6e-1728.95SEC14-like protein 1 OS=Homo sapiens OX=9606 GN=SEC14L1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1GFU62.6e-189100.00SEC14 cytosolic factor-like OS=Cucurbita moschata OX=3662 GN=LOC111453570 PE=4 S... [more]
A0A6J1IKR15.3e-18798.20SEC14 cytosolic factor-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114779... [more]
A0A6J1IN416.1e-18391.60SEC14 cytosolic factor-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114779... [more]
A0A0A0KMW15.2e-16687.09CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510980 P... [more]
A0A5A7SKD02.6e-16586.49SEC14 cytosolic factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
Match NameE-valueIdentityDescription
AT5G47730.15.4e-12366.04Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G55840.13.6e-11961.99Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G55840.24.3e-11264.51Sec14p-like phosphatidylinositol transfer family protein [more]
AT5G47730.27.8e-10669.17Sec14p-like phosphatidylinositol transfer family protein [more]
AT5G47730.37.8e-10669.17Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011074CRAL/TRIO, N-terminal domainSMARTSM01100CRAL_TRIO_N_2coord: 35..60
e-value: 4.5E-4
score: 29.5
IPR011074CRAL/TRIO, N-terminal domainPFAMPF03765CRAL_TRIO_Ncoord: 37..59
e-value: 1.2E-5
score: 25.4
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 79..247
e-value: 5.2E-31
score: 119.0
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 87..241
e-value: 1.5E-19
score: 70.3
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 79..239
score: 18.629185
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 91..241
e-value: 3.85877E-24
score: 94.3742
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 4..283
e-value: 5.8E-51
score: 175.4
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 81..241
NoneNo IPR availablePANTHERPTHR46226:SF5OS09G0258000 PROTEINcoord: 1..332
NoneNo IPR availablePANTHERPTHR46226FAMILY NOT NAMEDcoord: 1..332
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 37..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00516-RACarg00516-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane