Carg00467 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00467
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionEamA domain-containing protein
LocationCarg_Chr04: 7459823 .. 7465655 (+)
RNA-Seq ExpressionCarg00467
SyntenyCarg00467
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGTAACCACCGATAACTTACATTCTAATTCTTCTTCTTCTGCTTCTTAATTCTTCAACATCGTATTTTCTTCATCTCTCTGCATTTCTCTTCTCTAACAATCCCTTTACACGCCGAACCACCGGTTCTCCCGGTCCCCATTGTTCTTTTTCATCCCACCATTTCACTTTCCCCCCCTCCAAGATCCGTTAACTCTCTCGTTGTCCTATCGCCAACCGGTCAAATTTGAAGAAAAGCTTGAAGATCTACGAGAAGGACACCGATCCCTTTTCGAATTTCGTGATTCTTCTTGATGGGTTGGAGGTATAAGGCTGGTTTGTTTCTCATAGTGACTGTTGTGATCATATGGGTAACCTCAGCCGAAGTGACGCAGGTATGTGTTCGTTCTCTGTTTCGATTTGTTCTTGTACTTGAGTTCAATGTGGAAGATCTTGAGTTGTTTGCAGCTTAATTGATTGTTGAGCTTAGCTGGGATTTTGTTATGGTGGGCAGACTTTTTTTTGTACATTTGCTTATGCGTGCTGCAATCCACACACTGTTTGATTCTTAGTTTGGAACTTGTTTTCTGCATTTTCTTTTGGAGTTTCATGCTTTGTTTGCTGAATGATCTGTTGTTGGTTTGGGTTTTCCCTGTAGTTTGACTTTGGAATTTTGATTGAATTTGAGCTGGGAGTATTGGGATTTTGGTTGCTATGGTAGAATTATCTTTAGTCCATGAAACTAGTGTCATGTAGTAACAGCCCAAGTCCACCGCTAATAAATATTGTTCTCTTTGGGTTTTCCCTTCCGGGCTTCTCCTCAAGGGTTTAAAAACGCATCTCATAGGGAGAGGTTTCCACACCCTTATAAACAATGTTTCGTTCTCCTCCCTAACTGACGTGAAATCTCACAATCCCACCCCCCTTTGGAGCCCAGTGTCCTCGTTGACACTCGTTCCTTTCTCCAATCGATGTGGGACCCCCCAATCCACCCTCTTTGAGGCTTAACATCTTTGTTGACACACCGTCTTGTGTCCACCCCCCTTCGGAGCTCAGCCTCCTCGCCGGCACATTGCTTGGAGTCTGGCTTTGATGCTATTTGTAACAGCTCAAGCCTACCACTAACAAATATTGTTCTCTTTGGGTTTTCCCTCAAGGTTTTAAAGACGCATTTGCTAGGGAGAGGTTTCCACACCCTTATAAACAATGTTTCCTTCTCCTCCCCATCCAATGTGGGATCTTAGAATCCACCCCCTTTCGGGACCCAACATCCTTGCTGGCACACCATCTCGTATCCACCCCTCTTCGGGGCTCAGCCTCCTCACTGGCACATTGCCTAATGTCTAGATGTAATACAATTTGTAACAGATCAAGCCCACCGCTAACAAATATTGTCCTCTTTGGGCTTTTCCTTCTAAGTTTCCCCTCAAGGTTTTAAAAATGCGTCTGCTAGAGAGAGGTTTCCACACCCCTTATAAACAATGTTTTGTTCTCCTCCCCAACCGATATGGTATCTCACACTTGTAGATAAGAAACTCAAATACGTTTTTTCAAAGATTTGTTGAACTGCTTTAGAGATCCAGAATCAGGACACTATTTTCTGCTGGGCCTTCCATTTCAAAGCTGTTTGAATGGTGACATTTGTTTAAAAGATATGAAGCCTTTCGATGTTTCTAAAATTTGATCAAGTTTTATGGTTCCCTTTCTTTGCAGGACATTTTTACTGCTTATAAGCAGCCATTTGCCATAACATATCTTGGAGCTTCTCTCATGGTAGTGTACCTCCCAATAGCATTCCTTAAGGATTGGTTTTGTAGTTTGGTAAGAACCCACTCTTCTAAAAGTGAAGCGGAGACAAACTTGGATGTGGAACTTCAGGGGAGTTTCACTAGAAAAGACAGTGATGCTGATTTTTCAGCTCATTCTGAAGGAAGTCCCCTGGTTTCTAAAAACAAAGATGATCCATGTATATTGAAACAAGAGAAAGAGCTTACTAACAAGGAAATTGCTGCATATGGATTTTACATCGCCCCCATCTGGTTTGTAACCGAGGCAAGTTAGCACACGTCTTTGACTTATTTGGTTCGATAGATTATGATCCAATTTATTTGTCTTTTAGTCTGTGAGATCCCACGTCGGTTGGGGAGGAAAACTTAGCATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGATACGTTTTAAAACCTTGAGGGGAAGCCTGGAAGGGAAACCTAAAGAGGACTATATCTGCTAGCGGTGGTCTTGGGTTGTTACATAATCGGTTTAAAATTTTCTTAAGCATATTTATGCGATATTCATTATGTATGACATGATTTCAAATGAAATGTTCTCGATCATTTTTTAGAAATATGGGTGTAATAGCTCAAGTCTACCGTTAGCAAATATTGTTCTCTTTGGGCTTCCCCTTATGGTTTTAAAACATGTCTACTAGGGAGAGATTTTCACACCCTTATAAGAAACGTTTCGTTCCCCTCTCCAACCGATGTGGGATCTCGCAATGAGGCGATGTTCTTTGTCACTGATGAAGATCATAAGATTCCTTTGTGTCAACCCCTATAGCATTGTGAGATATCTCCTTGAGTTGACTGCCACCGAGGTGTATCGGACTATAAAATCTTCCATACGGAGGCATTGACGGGAATCATTCACATGAACTGTCTTTATGTTTCTTGCACTTTCATCCTTAATTTGCAAGTCTCTACCATCAGACATCTCTAATGTGTAGATCTCAGCGATCATCATAGGAACCTTGGATGATATGATATTCCTAATACTCACTTGCTTTTGCAGTATCTATCAAATGCTGCACTTGCACGGACCAGTGTCGCCAGTACAACAGTCTTATCCTCTACTTCTGGACTTTTTACTCTGTTCATTGGTGCTGCTCTTGGTCAAGATTCATTAAATATGGTCAAAGTTGTGGCTGTCTTTGTCAGTATGGCCGGTGTTATTATGACAACTCTTGGTAAAACTTGGGCCACTGATGAGTCACAACTAACTGCTTCTGAGTATGTGTCCTCTCTTTCTTTTGAATCACAGCTCTTTGCTTGGCTTGTAGATCTATGCTTGATTCATATAATTTCTGCCTTCATTAGTCAAGCTGGAGTGACAAACTATATGCATATAGCTTTATATTATTACTACTGCATGAACAATCATTGATAGAACACAAGCATTTAAAATTTAATGCTTTTACTAAGGATATTAATCCATCCTTATAACTGTTACTAAAACCATAAATCTTATTAGGCTACAGCCTTTGTGTGAGATCCCACATCGGTTGGAGAGAAGAATGAAACATTCCTTATAAGGGGTGGAAACCTCTCCCTAGTAGACGCATTTTAAAACTTTGAGGGGAAGCTCAAAAAGGAAAGTCCATATAGGACAATATTTGCTAGTGGTGTGATTGGGCTATATTACAAATGGTATCAGAGCCAGGCACCGGGCGGTGTGCCAACGAGGACGTTGGGCTTCCGGGGGGGGGGGGGGGAGTGGATTGTGAGATCCCACATTTGTTGGAGAGTGGAACGATGCATTCCCTATAAGAGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAATCTTGAGGGGAAGCCTAGAAGGGAAAGTCCAATGAGGACAATATTTGCTAGTGGTGGGATTAGACTGTTACAAATGGTATCAGAGCCAGGCATCGGGCGGCGTGCCAACGAGGACACTGGGCCTCCAAGGGGGTGGATTGTGAGATCTCACATCGGTCGGAGAGAGGAACGAAGCATTCCTTGTACGGGTGTGGAAACTTCTTCCTAGCATATGCATTTTAAAACCTGGAGAGAAAGCCCACAATATTTCTTATCGATAACTCTGGATATGTCATTTGTAACAAGTCGTTGGCCTAGCAATATAAGGCGTTTTTGTTATCCCTGATATTTAAAATTCTGGTGCAGCAACGAGCACTCTCTCATTGGAGATCTTTTTGGCCTACTCTCAGCCGTGTCGTACGGGCTATTCACCGGTAATAAAAGTGTATCCTTCTTGATATTCTTAAGATTTATGTATTGCTATTGATTTGATTAGTTCTTTGAATTCTTTCTGTTGTAGTGCTTCTCAAAAAGTTTGCTGGTGAAGAAGGAGAGAGGGTCGATGTTCAAAAACTGTTTGGATACATTGGTCTTTTCACACTCGTTACACTTTGGTGGCTAGGTAACTAAGATTAAGATCACTTTAGCATGCTTGAAATGCTGGTTGGCTCATGAGATTGGTATCATATCTGTTCATTTTTTGTTTCAGTTTGGCCATTGACAGCCATGGGAATAGAACCCAAGTTTTCGATTCCTCACTCTCTTAGAACGGAAGAAGTAGTTCTCGCTAATGGCTTCATCGGAAGCGTACTTTCTGATTACTTCTGGTTTGTCGAATCGAGTTGACCCGACCCGACCCTTCCAACGTGTTCTATTCACTCACTCGTAACAATCCCTTCTCACATTGGACCTTTTCTTTTGTATTCAGGGCACTCTGTGTCGTATGGACTACGCCGTTGGTGGCAACGTTGGGCATGTCGCTCACCATTCCGCTTGCCATGTTGGCTGACATGTTCATCCATGGTCGTCATTACTCAGCGGTTTACATGCTTGGCTCGACTCAGGTAACTAACTACCTTTGTGATTCTGACCATGAAAGTACCAATAGATTAGCATCTATAAATGTTGACTAGCCTCGAGTTATATATAATGACTGTATTTGAGTAAGAATAGATTAGCATCCATGGAAACCGAGCTTTCATTCAAAAAAATGAAAGAATAATACTAGCAGCAGATAATTACAAAGTTCCAACTCCATAACGCCCATAAATAGGCATTAAAACTCGCCATATTCTAAACTTCCTCTAAAAACCTCTTCATCGTTCTCTCGAGTTTTGATCCACATCCCGAATTGTTTAAGTATTTGTAATAGCCCAGGCTCACTGCTAGCAGATATTGTCTGCTTTAGCCCGTTACATATTGCCACCAGTCTTACGGTTTTAAAACGCATCTACTAGAGAGAGGTTTCCACACCCTTGTAATCACTGCTTCATTCCCCTATCCAATTGACGTGGGGTCTCACTATCCCCCTCTTGGAGGTCCAGCGTCCTTGTTGACGCACTGCCCAATGTCTGGCTTTGATACCATTTGTAACGGTCCAAACCCACCGCTAGCAGATATTTTCCACTTTGGCTCGTTACGTATCACCATTAGTCTCACGGTTTTAAAACACGTTTATTAGAGAGAGGTTTTCACACTCTTATAAGGACTGCTTCGTTCCTCGCTCCAACCGACATAAAATCTCACTATCCACCCTTAGTGGGGGTCCAACGGTCTCGTTGGTACATCACCTAGTGTCTGACTCTTATACCATTTGTAACAGTCCAAATCCACTACTAACAAATATTGTCAAACCCACCACTACCATTTGTATCATCGTCAGCCTCACGATTTTAAAACGCGTCTACTAGGGTGAGGTTTCTACACCCTTATAAGGTTTGCTTCGTTCCCCTCACTAACCGATGTGAGATCTCACACTCTTACACAAATCATTCAAACTAAAAGACATGTTACAATCAAATCCTTGAACAGTAGGCATCAGTATCTTTTATACCAATATCTCGAGTCGACATCTCGTGGAGACGGGTTACTGAATACTTTGTGTTGTGGCTTGCAGGTGTTTGCAGGATTTGTAATTGCAAATCTTTCAGAATGGTTCTCAAAGAAGCTGGGCTTGTAGCATTTGGAAATTCTTAATTCTCTCTCCTTCATCAATTGAATTGCAATGTAAATTCTTATTGAAAACTGTAAAATCTTCAATTTTGGGTT

mRNA sequence

GAAGTAACCACCGATAACTTACATTCTAATTCTTCTTCTTCTGCTTCTTAATTCTTCAACATCGTATTTTCTTCATCTCTCTGCATTTCTCTTCTCTAACAATCCCTTTACACGCCGAACCACCGGTTCTCCCGGTCCCCATTGTTCTTTTTCATCCCACCATTTCACTTTCCCCCCCTCCAAGATCCGTTAACTCTCTCGTTGTCCTATCGCCAACCGGTCAAATTTGAAGAAAAGCTTGAAGATCTACGAGAAGGACACCGATCCCTTTTCGAATTTCGTGATTCTTCTTGATGGGTTGGAGGTATAAGGCTGGTTTGTTTCTCATAGTGACTGTTGTGATCATATGGGTAACCTCAGCCGAAGTGACGCAGGACATTTTTACTGCTTATAAGCAGCCATTTGCCATAACATATCTTGGAGCTTCTCTCATGGTAGTGTACCTCCCAATAGCATTCCTTAAGGATTGGTTTTGTAGTTTGGTAAGAACCCACTCTTCTAAAAGTGAAGCGGAGACAAACTTGGATGTGGAACTTCAGGGGAGTTTCACTAGAAAAGACAGTGATGCTGATTTTTCAGCTCATTCTGAAGGAAGTCCCCTGTATCTATCAAATGCTGCACTTGCACGGACCAGTGTCGCCAGTACAACAGTCTTATCCTCTACTTCTGGACTTTTTACTCTGTTCATTGGTGCTGCTCTTGGTCAAGATTCATTAAATATGGTCAAAGTTGTGGCTGTCTTTGTCAGTATGGCCGGTGTTATTATGACAACTCTTGGTAAAACTTGGGCCACTGATGAGTCACAACTAACTGCTTCTGACAACGAGCACTCTCTCATTGGAGATCTTTTTGGCCTACTCTCAGCCGTGTCGTACGGGCTATTCACCGTGCTTCTCAAAAAGTTTGCTGGTGAAGAAGGAGAGAGGGTCGATGTTCAAAAACTGTTTGGATACATTGGTCTTTTCACACTCGTTACACTTTGGTGGCTAGTTTGGCCATTGACAGCCATGGGAATAGAACCCAAGTTTTCGATTCCTCACTCTCTTAGAACGGAAGAAGTAGTTCTCGCTAATGGCTTCATCGGAAGCGTACTTTCTGATTACTTCTGGGCACTCTGTGTCGTATGGACTACGCCGTTGGTGGCAACGTTGGGCATGTCGCTCACCATTCCGCTTGCCATGTTGGCTGACATGTTCATCCATGGTCGTCATTACTCAGCGGTTTACATGCTTGGCTCGACTCAGGTGTTTGCAGGATTTGTAATTGCAAATCTTTCAGAATGGTTCTCAAAGAAGCTGGGCTTGTAGCATTTGGAAATTCTTAATTCTCTCTCCTTCATCAATTGAATTGCAATGTAAATTCTTATTGAAAACTGTAAAATCTTCAATTTTGGGTT

Coding sequence (CDS)

ATGGGTTGGAGGTATAAGGCTGGTTTGTTTCTCATAGTGACTGTTGTGATCATATGGGTAACCTCAGCCGAAGTGACGCAGGACATTTTTACTGCTTATAAGCAGCCATTTGCCATAACATATCTTGGAGCTTCTCTCATGGTAGTGTACCTCCCAATAGCATTCCTTAAGGATTGGTTTTGTAGTTTGGTAAGAACCCACTCTTCTAAAAGTGAAGCGGAGACAAACTTGGATGTGGAACTTCAGGGGAGTTTCACTAGAAAAGACAGTGATGCTGATTTTTCAGCTCATTCTGAAGGAAGTCCCCTGTATCTATCAAATGCTGCACTTGCACGGACCAGTGTCGCCAGTACAACAGTCTTATCCTCTACTTCTGGACTTTTTACTCTGTTCATTGGTGCTGCTCTTGGTCAAGATTCATTAAATATGGTCAAAGTTGTGGCTGTCTTTGTCAGTATGGCCGGTGTTATTATGACAACTCTTGGTAAAACTTGGGCCACTGATGAGTCACAACTAACTGCTTCTGACAACGAGCACTCTCTCATTGGAGATCTTTTTGGCCTACTCTCAGCCGTGTCGTACGGGCTATTCACCGTGCTTCTCAAAAAGTTTGCTGGTGAAGAAGGAGAGAGGGTCGATGTTCAAAAACTGTTTGGATACATTGGTCTTTTCACACTCGTTACACTTTGGTGGCTAGTTTGGCCATTGACAGCCATGGGAATAGAACCCAAGTTTTCGATTCCTCACTCTCTTAGAACGGAAGAAGTAGTTCTCGCTAATGGCTTCATCGGAAGCGTACTTTCTGATTACTTCTGGGCACTCTGTGTCGTATGGACTACGCCGTTGGTGGCAACGTTGGGCATGTCGCTCACCATTCCGCTTGCCATGTTGGCTGACATGTTCATCCATGGTCGTCATTACTCAGCGGTTTACATGCTTGGCTCGACTCAGGTGTTTGCAGGATTTGTAATTGCAAATCTTTCAGAATGGTTCTCAAAGAAGCTGGGCTTGTAG

Protein sequence

MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWFCSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPLYLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFVIANLSEWFSKKLGL
Homology
BLAST of Carg00467 vs. NCBI nr
Match: KAG7031808.1 (hypothetical protein SDJN02_05849 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 652.9 bits (1683), Expect = 1.5e-183
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPLYLSNAALARTSVASTTV 120
           CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPLYLSNAALARTSVASTTV
Sbjct: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPLYLSNAALARTSVASTTV 120

Query: 121 LSSTSGLFTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHS 180
           LSSTSGLFTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHS
Sbjct: 121 LSSTSGLFTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHS 180

Query: 181 LIGDLFGLLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMG 240
           LIGDLFGLLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMG
Sbjct: 181 LIGDLFGLLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMG 240

Query: 241 IEPKFSIPHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADM 300
           IEPKFSIPHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADM
Sbjct: 241 IEPKFSIPHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADM 300

Query: 301 FIHGRHYSAVYMLGSTQVFAGFVIANLSEWFSKKLGL 338
           FIHGRHYSAVYMLGSTQVFAGFVIANLSEWFSKKLGL
Sbjct: 301 FIHGRHYSAVYMLGSTQVFAGFVIANLSEWFSKKLGL 337

BLAST of Carg00467 vs. NCBI nr
Match: KAG6608168.1 (hypothetical protein SDJN03_01510, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 634.4 bits (1635), Expect = 5.5e-178
Identity = 337/374 (90.11%), Postives = 337/374 (90.11%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPL----------------- 120
           CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPL                 
Sbjct: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPLVSKNKDDPCILKQEKEL 120

Query: 121 --------------------YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180
                               YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM
Sbjct: 121 TNKEIAAYGFYIAPIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180

Query: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240
           VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK
Sbjct: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240

Query: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300
           FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI
Sbjct: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300

Query: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 338
           GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV
Sbjct: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 360

BLAST of Carg00467 vs. NCBI nr
Match: XP_022941432.1 (uncharacterized vacuolar membrane protein YML018C-like [Cucurbita moschata])

HSP 1 Score: 632.5 bits (1630), Expect = 2.1e-177
Identity = 336/374 (89.84%), Postives = 336/374 (89.84%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPL----------------- 120
           CSLVRTHSSKSE ETNLDVELQGSFTRKDSDADFSAHSEGSPL                 
Sbjct: 61  CSLVRTHSSKSEEETNLDVELQGSFTRKDSDADFSAHSEGSPLVSKNKDDPCILKQEKEL 120

Query: 121 --------------------YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180
                               YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM
Sbjct: 121 TNKEIAAYGFYIAPIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180

Query: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240
           VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK
Sbjct: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240

Query: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300
           FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI
Sbjct: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300

Query: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 338
           GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV
Sbjct: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 360

BLAST of Carg00467 vs. NCBI nr
Match: XP_023524250.1 (uncharacterized vacuolar membrane protein YML018C-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630.9 bits (1626), Expect = 6.1e-177
Identity = 335/374 (89.57%), Postives = 336/374 (89.84%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPL----------------- 120
           CSLVRTHSSKS AETNLDVELQGSFTRKDSDADFSAHSEGSPL                 
Sbjct: 61  CSLVRTHSSKSGAETNLDVELQGSFTRKDSDADFSAHSEGSPLVSKNKDDPCILKQEKEL 120

Query: 121 --------------------YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180
                               YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM
Sbjct: 121 TNKEIAAYGFYIAPIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180

Query: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240
           VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGD+FGLLSAVSYGLFTVLLKK
Sbjct: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDIFGLLSAVSYGLFTVLLKK 240

Query: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300
           FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI
Sbjct: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300

Query: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 338
           GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV
Sbjct: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 360

BLAST of Carg00467 vs. NCBI nr
Match: XP_022981813.1 (uncharacterized vacuolar membrane protein YML018C-like [Cucurbita maxima])

HSP 1 Score: 628.6 bits (1620), Expect = 3.0e-176
Identity = 334/374 (89.30%), Postives = 335/374 (89.57%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPL----------------- 120
           CSLVR+HSSKS AETNLDVELQGSFTRKDSDADFSAHSEGSPL                 
Sbjct: 61  CSLVRSHSSKSGAETNLDVELQGSFTRKDSDADFSAHSEGSPLVSRNKDAPCILKQEKEL 120

Query: 121 --------------------YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180
                               YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM
Sbjct: 121 TNKEIAAYGFYIAPIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180

Query: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240
           VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK
Sbjct: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240

Query: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300
           FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI
Sbjct: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300

Query: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 338
           GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVF GFV
Sbjct: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFVGFV 360

BLAST of Carg00467 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.4e-37
Identity = 118/360 (32.78%), Postives = 187/360 (51.94%), Query Frame = 0

Query: 4   RYKAGLFLIVTVVIIWVTSAEVTQDIF--TAYKQPFAITYLGASLMVVYL------PIAF 63
           R+  GL ++  V+I+WV S+ +   IF   +Y++PF ITY   +  + YL       +  
Sbjct: 12  RWTLGLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYLFPTAKAVVVN 71

Query: 64  LKDWFCSLVRTHSSKSE----AETNLDVELQGSFTRKDSDADFSAHSEGSPLY------- 123
            KD   + V       E    +++N  V++             +   +   LY       
Sbjct: 72  YKDTGRANVHRELIMEEEGTGSDSNRSVDMTSPLLTNLEAGTHANQKKRLTLYETIKLSA 131

Query: 124 -----------LSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNMVKVVAVFVSM 183
                      ++NA+LA TSVAS T+LS+TS  FTLFIGA    +SL+  KV+  F+S 
Sbjct: 132 EFCILWFTANLVTNASLAFTSVASQTILSTTSSFFTLFIGAICHVESLSKSKVLGSFISF 191

Query: 184 AGVIMTTLG---KTWATDESQLTASDNE--HSLIGDLFGLLSAVSYGLFTVLLKKFAGEE 243
            G+IM T     + +    + ++  DN+    LIG+L  L  AV YG+++ LLK+  G+E
Sbjct: 192 VGIIMVTKSDSHQRYQRHIADVSGDDNDAVQVLIGNLLALAGAVLYGVYSTLLKREVGDE 251

Query: 244 GERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFIGSVLS 303
             RV+++  FG++GLF L+ LW  +  L   G EP FS+P   +   ++  N  I + +S
Sbjct: 252 -TRVNMKIFFGFVGLFNLLFLWPSLIVLDFFGWEP-FSLPKDPKVVVIIFVNCLI-TFVS 311

Query: 304 DYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFVIANLS 329
           D+ WA  ++ T+PL  T+G+S+TIPLAM  D+    +  SA+Y+ G+T +   F I N S
Sbjct: 312 DFCWAKAMLLTSPLTVTVGLSITIPLAMFGDVIFKHKTMSALYLFGATLILGSFFIINKS 368

BLAST of Carg00467 vs. ExPASy Swiss-Prot
Match: O94654 (Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC405.03c PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.5e-34
Identity = 106/341 (31.09%), Postives = 181/341 (53.08%), Query Frame = 0

Query: 4   RYKAGLFLIVTVVIIWVTSAEVTQDIF--TAYKQPFAITYLGASLMVVYLPIAFLKDWFC 63
           ++  G+ L++ VV +W+ S+ +T  +     +  PF ITY+     V Y     L  W+ 
Sbjct: 8   KHALGVVLLLFVVFLWLISSFLTSSLLDDDNFFSPFLITYINTGTFVFY-----LIPWYF 67

Query: 64  SLVRTHSSKSEAETNLDVELQGSF----TRKDSDADFSAHSEGS---------PLYLSNA 123
           S  +T   +  +E ++   +  S     TR +S   F   +  S           Y SN+
Sbjct: 68  SEKKTRKHRLMSELSMYESVHDSSFNLGTRPNSPLGFRQTAYLSLGFCIIWFAANYFSNS 127

Query: 124 ALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWATD 183
           +L  T+VAS T++SS SG FTL +G  +  +   + K++A+  S+ GVI+     T    
Sbjct: 128 SLGFTNVASFTIISSMSGFFTLGLGTIVNVERFTLSKLLALMASVGGVIIVV---TQDAK 187

Query: 184 ESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLVT 243
           ++ L  S      +G+ + LL+A+ YG ++V++K    EE   V  +  FG +GLF L+ 
Sbjct: 188 QADLNDSPPSRPALGNAYALLAALLYGCYSVMVKFHITEE-SCVSTRLFFGLVGLFDLIL 247

Query: 244 LWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGM 303
           LW  +  L   G+E +FS+P +     V++ N  I + +SDY W + ++ T+PL+ T+GM
Sbjct: 248 LWPFLIILHLYGVE-RFSLPSTTAGLIVLIINASI-TFVSDYLWVIAMLMTSPLLVTVGM 307

Query: 304 SLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFVIANLSE 330
           SL+IPLA+  D+ + G + +   +LGS  VFAGF++ N ++
Sbjct: 308 SLSIPLALFFDILLKGHYLNFSLILGSLLVFAGFIVVNYNQ 337

BLAST of Carg00467 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.0e-33
Identity = 105/346 (30.35%), Postives = 179/346 (51.73%), Query Frame = 0

Query: 8   GLFLIVTVVIIWVTSAEVTQDIF--TAYKQPFAITYLGASLMVVYLPIAFLKDWFCSLVR 67
           G+ L+  VV+ WV ++ +T ++    AY +PF +TYL  S   +YL       W     R
Sbjct: 13  GVLLLAVVVVFWVGASCLTNELLETNAYNKPFFLTYLNISSFALYLTPDL---WRIIQSR 72

Query: 68  THSSKSEAETNLDVELQGSFTR--------KDSDADFSAHSEG--------SPLY----- 127
             S +   E  L +  Q SF+           + ++ S+ ++         S L+     
Sbjct: 73  RKSLQERTERTLPIHTQESFSEFLPLLSSTPSTSSNLSSIADTKVKDTMRLSLLFCVLWF 132

Query: 128 ----LSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTT 187
                +NAAL+ T+VAS+T+LSSTS  FTLF+  +LG ++ +  K++ +FVS+ G+I+  
Sbjct: 133 VANLAANAALSYTTVASSTILSSTSSFFTLFLATSLGIETFSTKKLLGLFVSLFGIILIV 192

Query: 188 LGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGY 247
           +    +  +  ++AS     L+G+   LL ++ Y ++T LLK     +G R+D+Q   GY
Sbjct: 193 MQS--SKQQDSVSASS---FLVGNTLALLGSLGYSVYTTLLKYEISSKGLRLDIQMFLGY 252

Query: 248 IGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFIGSVLSDYFWALCVVWTT 307
           +G+FT +  W ++  L    +E  F +P +     +V+ N  I   +SDYFW   ++ T+
Sbjct: 253 VGIFTFLLFWPILIILDITHME-TFELPSNFHISFLVMLNCII-IFVSDYFWCKALILTS 312

Query: 308 PLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFVIAN 327
           PLV T+ ++ TIPLAM AD       ++  Y++G   +F  F + N
Sbjct: 313 PLVVTVALTFTIPLAMFADFVWREAFFTPWYIIGVIFIFVSFFLVN 348

BLAST of Carg00467 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.9e-24
Identity = 114/417 (27.34%), Postives = 182/417 (43.65%), Query Frame = 0

Query: 4   RYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFL--KDW-- 63
           R   G+ +++ V +IWV S+E+T  +FT Y +PF  T+   S+ V+YL + F+  K W  
Sbjct: 68  RMALGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYL-LGFIVWKPWRQ 127

Query: 64  ----------------------FCSL---------------VRTHSSKSE--AETNLDVE 123
                                  C+                V+ H   SE    TN+D E
Sbjct: 128 QCTRGFRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPENTNIDTE 187

Query: 124 LQGSFTR--------------------KDSDADFSAHSEGSPL----------------- 183
                +R                    K S   +    E   L                 
Sbjct: 188 KIPKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKEQESLLKTVGKLTATQVAKISF 247

Query: 184 ------YLSN----AALARTSVASTTVLSSTSGLFTLFIGAAL---GQDSLNMVKVVAVF 243
                 +L+N     AL+ T VA   +LSSTSGLFTL + A       D   + K++AV 
Sbjct: 248 FFCFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNSGDRFTLSKLLAVI 307

Query: 244 VSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKKFAGEEGE 303
           +S+ GV++  L           +      + IG ++ L+ A+ Y ++ V++K+    E +
Sbjct: 308 LSIGGVVLVNLSG---------SEKSPGRNTIGSIWSLVGAMLYAVYIVMIKRKVDRE-D 367

Query: 304 RVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFIGSVLSDY 328
           ++D+   FG++GLF L+ LW   + L   G E  F  P+ +    +V+ NG IG+VLS++
Sbjct: 368 KLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKVVLMCIVI-NGLIGTVLSEF 427

BLAST of Carg00467 vs. ExPASy Swiss-Prot
Match: Q8R314 (Solute carrier family 35 member F5 OS=Mus musculus OX=10090 GN=Slc35f5 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.5e-24
Identity = 116/420 (27.62%), Postives = 179/420 (42.62%), Query Frame = 0

Query: 4   RYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYL------------ 63
           R   G+ +++ V +IWV S+E+T  +FT Y +PF  T+   S+ V+YL            
Sbjct: 68  RMALGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIIWKPWRQQ 127

Query: 64  --------PIAFLKD---WFCSL-----------------VRTHSSKSE--AETNLDVEL 123
                   P AF  D   +F +                  V+ H   SE    TN+  E 
Sbjct: 128 CTRGFRGKPAAFFADAEGYFAACTTDTSMSSSLSEPLYVPVKFHDLPSEKLESTNIGTEK 187

Query: 124 QGSFTRKDSDADFSAHSEGSPLYLSNA--------------------------------- 183
               T K S   FS   E   L  S+A                                 
Sbjct: 188 ----TPKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKDQESILKTVGKLTATQVAK 247

Query: 184 ------------------ALARTSVASTTVLSSTSGLFTLFIGAAL---GQDSLNMVKVV 243
                             AL+ T VA   +LSSTSGLFTL + A       D   + K++
Sbjct: 248 ISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLL 307

Query: 244 AVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKKFAGE 303
           AV +S+ GV++  L        S+ +A       IG ++ L  A+ Y ++ V++K+    
Sbjct: 308 AVILSIGGVVLVNL------SGSEKSAG---RDTIGSIWSLAGAMFYAVYIVMIKRKVDR 367

Query: 304 EGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFIGSVL 328
           E +++D+   FG++GLF L+ LW   + L   G E  F  P+ +     ++ NG IG+VL
Sbjct: 368 E-DKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKV-VLLCIIINGLIGTVL 427

BLAST of Carg00467 vs. ExPASy TrEMBL
Match: A0A6J1FMF1 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita moschata OX=3662 GN=LOC111446727 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.0e-177
Identity = 336/374 (89.84%), Postives = 336/374 (89.84%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPL----------------- 120
           CSLVRTHSSKSE ETNLDVELQGSFTRKDSDADFSAHSEGSPL                 
Sbjct: 61  CSLVRTHSSKSEEETNLDVELQGSFTRKDSDADFSAHSEGSPLVSKNKDDPCILKQEKEL 120

Query: 121 --------------------YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180
                               YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM
Sbjct: 121 TNKEIAAYGFYIAPIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180

Query: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240
           VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK
Sbjct: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240

Query: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300
           FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI
Sbjct: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300

Query: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 338
           GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV
Sbjct: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 360

BLAST of Carg00467 vs. ExPASy TrEMBL
Match: A0A6J1J340 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=3661 GN=LOC111480848 PE=3 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.5e-176
Identity = 334/374 (89.30%), Postives = 335/374 (89.57%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHSEGSPL----------------- 120
           CSLVR+HSSKS AETNLDVELQGSFTRKDSDADFSAHSEGSPL                 
Sbjct: 61  CSLVRSHSSKSGAETNLDVELQGSFTRKDSDADFSAHSEGSPLVSRNKDAPCILKQEKEL 120

Query: 121 --------------------YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180
                               YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM
Sbjct: 121 TNKEIAAYGFYIAPIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLNM 180

Query: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240
           VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK
Sbjct: 181 VKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKK 240

Query: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300
           FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI
Sbjct: 241 FAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFI 300

Query: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGFV 338
           GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVF GFV
Sbjct: 301 GSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFVGFV 360

BLAST of Carg00467 vs. ExPASy TrEMBL
Match: A0A5A7V8J8 (Putative vacuolar membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold205G001530 PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.0e-169
Identity = 324/387 (83.72%), Postives = 333/387 (86.05%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSK----------------SEAETNLDVELQGSFTRKDSDADFSAHSEGSPL- 120
           C+LV+ HSSK                S AETNLDVELQG+FTRKDSDADFS H+E SPL 
Sbjct: 61  CNLVKRHSSKSGKNAESFSETCALKQSGAETNLDVELQGNFTRKDSDADFSTHAEESPLV 120

Query: 121 ---------------------------------YLSNAALARTSVASTTVLSSTSGLFTL 180
                                            YLSNAALARTSVASTTVLSSTSGLFTL
Sbjct: 121 SRNKDDPYILKQEKELTNKEIATYGFYIAPIWFYLSNAALARTSVASTTVLSSTSGLFTL 180

Query: 181 FIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLS 240
           FIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWA+DESQLTASDNEHSLIGDLFGLLS
Sbjct: 181 FIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWASDESQLTASDNEHSLIGDLFGLLS 240

Query: 241 AVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHS 300
           AVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTL+TLWWLVWPLTA+GIEPKFSIPHS
Sbjct: 241 AVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLITLWWLVWPLTALGIEPKFSIPHS 300

Query: 301 LRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAV 338
           LRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMF+HGRHYSAV
Sbjct: 301 LRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFLHGRHYSAV 360

BLAST of Carg00467 vs. ExPASy TrEMBL
Match: A0A1S3CR91 (uncharacterized vacuolar membrane protein YML018C OS=Cucumis melo OX=3656 GN=LOC103503845 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.3e-169
Identity = 324/390 (83.08%), Postives = 333/390 (85.38%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSK----------------SEAETNLDVELQGSFTRKDSDADFSAHSEGSPL- 120
           C+LV+ HSSK                S AETNLDVELQG+FTRKDSDADFS H+E SPL 
Sbjct: 61  CNLVKRHSSKSGKNAESFSETCALKQSGAETNLDVELQGNFTRKDSDADFSTHAEESPLV 120

Query: 121 ------------------------------------YLSNAALARTSVASTTVLSSTSGL 180
                                               YLSNAALARTSVASTTVLSSTSGL
Sbjct: 121 SRNKDDPYILKQEKELTNKEIATYGFYIAPIWFVTEYLSNAALARTSVASTTVLSSTSGL 180

Query: 181 FTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFG 240
           FTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWA+DESQLTASDNEHSLIGDLFG
Sbjct: 181 FTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWASDESQLTASDNEHSLIGDLFG 240

Query: 241 LLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSI 300
           LLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTL+TLWWLVWPLTA+GIEPKFSI
Sbjct: 241 LLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLITLWWLVWPLTALGIEPKFSI 300

Query: 301 PHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHY 338
           PHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMF+HGRHY
Sbjct: 301 PHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFLHGRHY 360

BLAST of Carg00467 vs. ExPASy TrEMBL
Match: A0A6J1F9M5 (uncharacterized vacuolar membrane protein YML018C isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443551 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.5e-168
Identity = 320/375 (85.33%), Postives = 329/375 (87.73%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLI TVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF
Sbjct: 1   MGWRYKAGLFLIATVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60

Query: 61  CSLVRTHSSKSEAETNLDVELQGSFTRKDSDADFSAHS-EGSPL---------------- 120
           C+LV+ HSSKS A+TNLDVELQGSFTRKDSDADFS H+ E SPL                
Sbjct: 61  CNLVKNHSSKSGADTNLDVELQGSFTRKDSDADFSTHAVEESPLVSRNKDDPYILKQEKE 120

Query: 121 ---------------------YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLN 180
                                YLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSL+
Sbjct: 121 LTNKEIAAYGFYIAPIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGAALGQDSLD 180

Query: 181 MVKVVAVFVSMAGVIMTTLGKTWATDESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLK 240
           M+KVVAVFVSMAGV+MTTLGKTWA DESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLK
Sbjct: 181 MIKVVAVFVSMAGVVMTTLGKTWAADESQLTASDNEHSLIGDLFGLLSAVSYGLFTVLLK 240

Query: 241 KFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGF 300
           KFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTA+GIEPKFSIPHSLRTEEVVLANGF
Sbjct: 241 KFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTALGIEPKFSIPHSLRTEEVVLANGF 300

Query: 301 IGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIHGRHYSAVYMLGSTQVFAGF 338
           IGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADM IHGRHYSAVY+LGSTQVFAGF
Sbjct: 301 IGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMLIHGRHYSAVYILGSTQVFAGF 360

BLAST of Carg00467 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 506.9 bits (1304), Expect = 1.2e-143
Identity = 280/394 (71.07%), Postives = 308/394 (78.17%), Query Frame = 0

Query: 1   MGWRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPIAFLKDWF 60
           MGWRYKAGLFLI TVVIIWVTSAEVTQDIFTAYKQPFA+TYLGASLM+VYLP+AFLKDW 
Sbjct: 1   MGWRYKAGLFLIGTVVIIWVTSAEVTQDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWL 60

Query: 61  CSLVRTHSSKSE---AETN--------------LDVELQGSFTRKDSDADFSAHSEGS-- 120
           C  +   SSKS    A T+              +++ LQG+ T+KDS+   S+H E    
Sbjct: 61  CRYLDRRSSKSNKIPALTDDSSVELGSPLRHKIIEMGLQGTITKKDSEEHLSSHEEEDER 120

Query: 121 PL-------------------------------------YLSNAALARTSVASTTVLSST 180
           PL                                     YLSNAALARTSVASTTVLSST
Sbjct: 121 PLIGKVREEVQTLKQRKEITTKQIAMYGLYLAPIWFVTEYLSNAALARTSVASTTVLSST 180

Query: 181 SGLFTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWATDESQLTASDN-EHSLIG 240
           SGLFTLFIG  LGQD+LN+ KVVAVFVSMAGV+MTTLGKTWA+DESQL +S N E SL+G
Sbjct: 181 SGLFTLFIGVFLGQDTLNLSKVVAVFVSMAGVVMTTLGKTWASDESQLNSSLNGERSLMG 240

Query: 241 DLFGLLSAVSYGLFTVLLKKFAGEEGERVDVQKLFGYIGLFTLVTLWWLVWPLTAMGIEP 300
           DLFGLLSAVSYGLFTVLLKKFAGEEGE VDVQKLFGYIGLFTLV LWWLVWPLTA+GIEP
Sbjct: 241 DLFGLLSAVSYGLFTVLLKKFAGEEGEGVDVQKLFGYIGLFTLVALWWLVWPLTALGIEP 300

Query: 301 KFSIPHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLGMSLTIPLAMLADMFIH 338
           KF+IPHS++ +EVVLANGFIGSVLSDYFWAL VVWTTPLVATLGMSLTIPLAM+ADM IH
Sbjct: 301 KFTIPHSVKVDEVVLANGFIGSVLSDYFWALSVVWTTPLVATLGMSLTIPLAMVADMMIH 360

BLAST of Carg00467 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 107.5 bits (267), Expect = 2.2e-23
Identity = 106/407 (26.04%), Postives = 177/407 (43.49%), Query Frame = 0

Query: 3   WRYKAGLFLIVTVVIIWVTSAEVTQDIFTAYKQPFAITYLGASLMVVYLPI----AFLKD 62
           WR+  GL  +  V  IW+ ++ V Q +  A   PF IT++  SL VVYLP+     +L+D
Sbjct: 6   WRWVLGLIYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIGRYLED 65

Query: 63  WFCSLVRTHSSKS------EAE-------------------------------------- 122
            + SL+   S +S      E+E                                      
Sbjct: 66  AYGSLLFWRSKRSHLMELVESEKAVLLGKDVSGVKSDATESSGLVVREQEISEGGNGIES 125

Query: 123 --TNLDVELQGSFTRKDSDADFS---AHSEG--------------SPLYL-----SNAAL 182
              N+++E+  S    D  +  S      +G               P +       N +L
Sbjct: 126 GLENIELEIDASVNVSDGASGVSNKGLDEKGRWTRMRVAKVSLVICPFWFLAQLTFNVSL 185

Query: 183 ARTSVASTTVLSSTSGLFTLFIGAALGQDSLNMVKVVAVFVSMAGVIMTTLGKTWATDES 242
             T+V S T+LSS S LFT  +      +    +K+ +V + M+G I+ ++G     D  
Sbjct: 186 KYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIVSMG-----DSE 245

Query: 243 QLTASDNEHSLIGDLFGLLSAVSYGLFTVLLKKFAGEEGE---RVDVQKLFGYIGLFTLV 302
             + +  ++ L+GD+  L+SA  Y ++  L++K   ++ E   RV + +  G++GLF   
Sbjct: 246 SKSNAVAKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERNGRVSMAQFLGFLGLFNFF 305

Query: 303 TLWWLVWPLTAMGIEPKFSIPHSLRTEEVVLANGFIGSVLSDYFWALCVVWTTPLVATLG 334
              +L   L     + +     +L+   +V+  G + +VLSDY WA  V+ TT  VAT G
Sbjct: 306 I--FLPPALILNFTKRERFNALTLKQFGLVVGKGLLDNVLSDYLWAKAVLLTTTTVATAG 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031808.11.5e-183100.00hypothetical protein SDJN02_05849 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6608168.15.5e-17890.11hypothetical protein SDJN03_01510, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022941432.12.1e-17789.84uncharacterized vacuolar membrane protein YML018C-like [Cucurbita moschata][more]
XP_023524250.16.1e-17789.57uncharacterized vacuolar membrane protein YML018C-like [Cucurbita pepo subsp. pe... [more]
XP_022981813.13.0e-17689.30uncharacterized vacuolar membrane protein YML018C-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q037303.4e-3732.78Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (s... [more]
O946543.5e-3431.09Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ... [more]
Q040831.0e-3330.35Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cere... [more]
A6QL921.9e-2427.34Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1[more]
Q8R3142.5e-2427.62Solute carrier family 35 member F5 OS=Mus musculus OX=10090 GN=Slc35f5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FMF11.0e-17789.84uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita moschata OX=... [more]
A0A6J1J3401.5e-17689.30uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=36... [more]
A0A5A7V8J81.0e-16983.72Putative vacuolar membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3CR912.3e-16983.08uncharacterized vacuolar membrane protein YML018C OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1F9M51.5e-16885.33uncharacterized vacuolar membrane protein YML018C isoform X2 OS=Cucurbita moscha... [more]
Match NameE-valueIdentityDescription
AT4G32140.11.2e-14371.07EamA-like transporter family [more]
AT3G07080.12.2e-2326.04EamA-like transporter family [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR23051:SF12OSJNBA0070O11.5 PROTEINcoord: 1..103
NoneNo IPR availablePANTHERPTHR23051:SF12OSJNBA0070O11.5 PROTEINcoord: 104..336
NoneNo IPR availablePANTHERPTHR23051SOLUTE CARRIER FAMILY 35, MEMBER F5coord: 1..103
NoneNo IPR availablePANTHERPTHR23051SOLUTE CARRIER FAMILY 35, MEMBER F5coord: 104..336
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 103..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00467-RACarg00467-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane