Carg00449 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg00449 vs. NCBI nr
Match: KAG7031790.1 (SPX domain-containing protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 492.3 bits (1266), Expect = 2.5e-135
Identity = 251/251 (100.00%), Postives = 251/251 (100.00%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL
Sbjct: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60

Query: 61  LEEELDKFNRFFVSKEEYYVIKWSERVAEIGDSDERLMKVGRDIVDFHGEMVLLENYSAL 120
           LEEELDKFNRFFVSKEEYYVIKWSERVAEIGDSDERLMKVGRDIVDFHGEMVLLENYSAL
Sbjct: 61  LEEELDKFNRFFVSKEEYYVIKWSERVAEIGDSDERLMKVGRDIVDFHGEMVLLENYSAL 120

Query: 121 NYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLS 180
           NYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLS
Sbjct: 121 NYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLS 180

Query: 181 SAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKEIRG 240
           SAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKEIRG
Sbjct: 181 SAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKEIRG 240

Query: 241 GSSTIDVFSWL 252
           GSSTIDVFSWL
Sbjct: 241 GSSTIDVFSWL 251

BLAST of Carg00449 vs. NCBI nr
Match: KAG6608151.1 (SPX domain-containing protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 485.0 bits (1247), Expect = 4.0e-133
Identity = 250/254 (98.43%), Postives = 251/254 (98.82%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKFWKILCILIESTLPEWR+EFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL
Sbjct: 1   MKFWKILCILIESTLPEWRDEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60

Query: 61  LEEELDKFNRFFVSKEEYYVIKWS---ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120
           LEEELDKFNRFFVSKEEYYVIKWS   ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY
Sbjct: 61  LEEELDKFNRFFVSKEEYYVIKWSLLQERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120

Query: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180
           SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK
Sbjct: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180

Query: 181 DLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKE 240
           DLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKE
Sbjct: 181 DLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKE 240

Query: 241 IRGGSSTIDVFSWL 252
           IRGGSSTIDVFSWL
Sbjct: 241 IRGGSSTIDVFSWL 254

BLAST of Carg00449 vs. NCBI nr
Match: XP_022980907.1 (SPX domain-containing protein 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 472.6 bits (1215), Expect = 2.1e-129
Identity = 244/254 (96.06%), Postives = 248/254 (97.64%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKFWKILCILIESTLPEWR+EFISYKKLKKQLKLMYPDKDG PKRLKFDGEASMEVFLNL
Sbjct: 1   MKFWKILCILIESTLPEWRDEFISYKKLKKQLKLMYPDKDGKPKRLKFDGEASMEVFLNL 60

Query: 61  LEEELDKFNRFFVSKEEYYVIKWS---ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120
           LEEELDKFN+FFVSKEEYYVIKWS   ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY
Sbjct: 61  LEEELDKFNQFFVSKEEYYVIKWSLLQERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120

Query: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180
           SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK
Sbjct: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180

Query: 181 DLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKE 240
           DLSSAAETVNEEGR+ GSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSAL+ALKE
Sbjct: 181 DLSSAAETVNEEGRRGGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALKALKE 240

Query: 241 IRGGSSTIDVFSWL 252
           IRGGSSTID FSWL
Sbjct: 241 IRGGSSTIDDFSWL 254

BLAST of Carg00449 vs. NCBI nr
Match: XP_022941364.1 (SPX domain-containing protein 2-like isoform X1 [Cucurbita moschata] >XP_022941365.1 SPX domain-containing protein 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 472.6 bits (1215), Expect = 2.1e-129
Identity = 244/254 (96.06%), Postives = 248/254 (97.64%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKFWKILCILIESTLPEWR+EFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL
Sbjct: 1   MKFWKILCILIESTLPEWRDEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60

Query: 61  LEEELDKFNRFFVSKEEYYVIKWS---ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120
           LEEELDKFN+FFVSKEEYYVIKWS   ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY
Sbjct: 61  LEEELDKFNQFFVSKEEYYVIKWSLLQERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120

Query: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180
           SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK
Sbjct: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180

Query: 181 DLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKE 240
           DLSSA+ETVNEEG   GSEAKTASESKEKVLNIPEDLEEIE+MESMYMKLTLSALEALKE
Sbjct: 181 DLSSASETVNEEGIMGGSEAKTASESKEKVLNIPEDLEEIEHMESMYMKLTLSALEALKE 240

Query: 241 IRGGSSTIDVFSWL 252
           IRGGSSTIDVFSWL
Sbjct: 241 IRGGSSTIDVFSWL 254

BLAST of Carg00449 vs. NCBI nr
Match: XP_023524280.1 (SPX domain-containing protein 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 471.5 bits (1212), Expect = 4.6e-129
Identity = 242/254 (95.28%), Postives = 248/254 (97.64%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKFWKILCILIESTLPEWR+EFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL
Sbjct: 79  MKFWKILCILIESTLPEWRDEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 138

Query: 61  LEEELDKFNRFFVSKEEYYVIKWS---ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120
           LEEELDKFN+FFVSKEEYYVIKWS   ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY
Sbjct: 139 LEEELDKFNQFFVSKEEYYVIKWSLLQERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 198

Query: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180
           SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECE +LDLLFFKK
Sbjct: 199 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECETILDLLFFKK 258

Query: 181 DLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKE 240
           DLSSAAETVNE+GR+ GSEA TASESKEKVLNIPEDLEEIEYMESMYMKLTLSAL+ALKE
Sbjct: 259 DLSSAAETVNEKGRRGGSEANTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALKALKE 318

Query: 241 IRGGSSTIDVFSWL 252
           IRGGSSTIDVFSWL
Sbjct: 319 IRGGSSTIDVFSWL 332

BLAST of Carg00449 vs. ExPASy Swiss-Prot
Match: O48781 (SPX domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=SPX2 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.9e-64
Identity = 140/265 (52.83%), Postives = 186/265 (70.19%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGN--PKRLKFD--------- 60
           MKF K L   IE TLPEWR++F+SYK+LKK+LKLM P    N   KR + D         
Sbjct: 1   MKFGKSLSNQIEETLPEWRDKFLSYKELKKKLKLMEPRSVENRPNKRSRSDSNSVDTDPT 60

Query: 61  -GEASMEV-FLNLLEEELDKFNRFFVSKEEYYVIKWSE---RVAEIGDSDERLMKVGRDI 120
            G    E+ F++LLE+EL+KFN FFV +EE Y+I+  E   +VA+  +S+E ++ + ++I
Sbjct: 61  VGMTKEELDFISLLEDELEKFNSFFVEQEEEYIIRLKELKDQVAKAKNSNEEMINIKKEI 120

Query: 121 VDFHGEMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLK 180
           VDFHGEMVLL NYSALNYTGLAKILKKYDKR+G LIR+PFI+KVL++PF++TD+L   +K
Sbjct: 121 VDFHGEMVLLMNYSALNYTGLAKILKKYDKRTGALIRLPFIQKVLQEPFFTTDLLNTFVK 180

Query: 181 ECEMVLDLLFFKKDLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMY 240
           ECE +LD LF     S+ +  ++EEG    S          ++L +P++L EIEYMES+Y
Sbjct: 181 ECEAMLDRLF----PSNKSRNLDEEGEPTTSGMVKTGTDDSELLRVPKELSEIEYMESLY 240

Query: 241 MKLTLSALEALKEIRGGSSTIDVFS 250
           MK T+SAL+ LKEIR GSST+ VFS
Sbjct: 241 MKSTVSALKVLKEIRSGSSTVSVFS 261

BLAST of Carg00449 vs. ExPASy Swiss-Prot
Match: Q69XJ0 (SPX domain-containing protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SPX1 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 7.3e-61
Identity = 145/272 (53.31%), Postives = 183/272 (67.28%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPK-----RLKFDG---EA 60
           MKF K L   I  TLPEWR++F+SYK LKK+LKL+     G  +     R+  DG   EA
Sbjct: 1   MKFGKSLSSQIVETLPEWRDKFLSYKDLKKRLKLIGGGGGGEERQAKRARVAADGGEEEA 60

Query: 61  SMEV-------FLNLLEEELDKFNRFFVSKEEYYVIKWSE---RVAEIG--DSDERLMKV 120
           +          F+ LLE ELDKFN FFV KEE Y+I+  E   RVA     +S E LM+V
Sbjct: 61  AAAAMTPEEAGFMRLLEAELDKFNSFFVEKEEEYIIRQKELQDRVARAAGRESKEELMRV 120

Query: 121 GRDIVDFHGEMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLG 180
            ++IVDFHGEMVLLENYSALNYTGL KILKKYDKR+G LIR+PFI+KVL+QPF++TD+L 
Sbjct: 121 RKEIVDFHGEMVLLENYSALNYTGLVKILKKYDKRTGALIRLPFIQKVLQQPFFTTDLLY 180

Query: 181 QLLKECEMVLDLLFFKKDLSSAAETVNEEGRQVGSEAKTASESKEKVLN---IPEDLEEI 240
           +L+K+CE +LD L    +LS +    +E+GR   +     S     ++N   IPE L+EI
Sbjct: 181 KLVKQCEAMLDQLLPSNELSVS----SEDGRGDSTNEDKPSNPSSSLVNGGTIPE-LDEI 240

Query: 241 EYMESMYMKLTLSALEALKEIRGGSSTIDVFS 250
           EYMESMYMK T++AL +LKEIR GSST+  FS
Sbjct: 241 EYMESMYMKGTVAALRSLKEIRSGSSTVSAFS 267

BLAST of Carg00449 vs. ExPASy Swiss-Prot
Match: B8B4D0 (SPX domain-containing protein 1 OS=Oryza sativa subsp. indica OX=39946 GN=SPX1 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.8e-60
Identity = 144/272 (52.94%), Postives = 182/272 (66.91%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPK-----RLKFDG---EA 60
           MKF K L   I  TLPEWR++F+SYK LKK+LKL+     G  +     R+  DG   EA
Sbjct: 1   MKFGKSLSSQIVETLPEWRDKFLSYKDLKKRLKLIGGGGGGEERQAKRARVAADGGEEEA 60

Query: 61  SMEV-------FLNLLEEELDKFNRFFVSKEEYYVIKWSE---RVAEIG--DSDERLMKV 120
           +          F+ LLE ELDKFN FFV KEE Y+I+  E   RVA     +S E LM+V
Sbjct: 61  AAAAMTPEEAGFMRLLEAELDKFNSFFVEKEEEYIIRQKELQDRVARAAGRESKEELMRV 120

Query: 121 GRDIVDFHGEMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLG 180
            ++IVDFHGEMVLLENYSALNYTGL KILKKYDKR+G LIR+PFI+KVL+QPF++TD+L 
Sbjct: 121 RKEIVDFHGEMVLLENYSALNYTGLVKILKKYDKRTGALIRLPFIQKVLQQPFFTTDLLY 180

Query: 181 QLLKECEMVLDLLFFKKDLSSAAETVNEEGRQVGSEAKTASESKEKVLN---IPEDLEEI 240
           +L+K+CE +LD L    +L  +    +E+GR   +     S     ++N   IPE L+EI
Sbjct: 181 KLVKQCEAMLDQLLPSNELPVS----SEDGRGDSTNEDKPSNPSSSLVNGGTIPE-LDEI 240

Query: 241 EYMESMYMKLTLSALEALKEIRGGSSTIDVFS 250
           EYMESMYMK T++AL +LKEIR GSST+  FS
Sbjct: 241 EYMESMYMKGTVAALRSLKEIRSGSSTVSAFS 267

BLAST of Carg00449 vs. ExPASy Swiss-Prot
Match: Q8LBH4 (SPX domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=SPX1 PE=1 SV=2)

HSP 1 Score: 230.3 bits (586), Expect = 2.4e-59
Identity = 132/258 (51.16%), Postives = 180/258 (69.77%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNP-KRLKFD----GEASME 60
           MKF K L   IE TLPEW+++F+SYK+LKK+LKL+       P KRL+ D    G +  E
Sbjct: 1   MKFGKSLSNQIEQTLPEWQDKFLSYKELKKRLKLIGSKTADRPVKRLRLDEFSVGISKEE 60

Query: 61  V-FLNLLEEELDKFNRFFVSKEEYYVIKWSE---RVAEIGDSDERLMKVGRDIVDFHGEM 120
           + F+ LLE+EL+KFN FFV KEE Y+I+  E   R+A+  DS E+++K+ ++IVDFHGEM
Sbjct: 61  INFIQLLEDELEKFNNFFVEKEEEYIIRLKEFRDRIAKAKDSMEKMIKIRKEIVDFHGEM 120

Query: 121 VLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLD 180
           VLLENYSALNYTGL KILKKYDKR+G+L+R+PFI+KVL+QPFY+TD+L +L+KE E +LD
Sbjct: 121 VLLENYSALNYTGLVKILKKYDKRTGDLMRLPFIQKVLQQPFYTTDLLFKLVKESEAMLD 180

Query: 181 LLFFKKDLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSA 240
            +F                         A+E++ ++  I  +L E ++MES++MK T++A
Sbjct: 181 QIF------------------------PANETESEI--IQAELSEHKFMESLHMKSTIAA 232

Query: 241 LEALKEIRGGSSTIDVFS 250
           L  LKEIR GSST+ VFS
Sbjct: 241 LRVLKEIRSGSSTVSVFS 232

BLAST of Carg00449 vs. ExPASy Swiss-Prot
Match: A2X254 (SPX domain-containing protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=SPX2 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.5e-50
Identity = 125/266 (46.99%), Postives = 164/266 (61.65%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGN--PKRLKFDGEASMEV-- 60
           MKF K L   I    PEWR+ F+SYK LKK+L L+     G    KR +  G  ++ V  
Sbjct: 1   MKFGKSLSSQIVEMQPEWRDNFLSYKDLKKRLNLISGGAAGERASKRRRVGGATAVTVTA 60

Query: 61  ------------FLNLLEEELDKFNRFFVSKEEYYVIKWSERVAEIGDSDERLMKVGRDI 120
                       F+ LL+ ELDKFN FF+ KEE YVIK  E       S E +M+V ++I
Sbjct: 61  AAAGGMTLEQAGFVGLLDAELDKFNFFFLEKEEEYVIKQKELRERKMASAEEVMRVRKEI 120

Query: 121 VDFHGEMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLK 180
           VD HGEMVLLENYSALNYTGL KILKKYDKR+G +IR+PF++KVL+QPF++TD+L +L+K
Sbjct: 121 VDLHGEMVLLENYSALNYTGLVKILKKYDKRTGSMIRLPFVQKVLQQPFFTTDLLYKLVK 180

Query: 181 ECEMVLDLLFFKKDLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMY 240
           ECE +LD L    + S A+E   ++       +K +S S      +P + E+     S  
Sbjct: 181 ECEEMLDQLMPTNEHSVASEDGKDDSEGEEKGSKPSSSSSANGGAVPGEAED---ERSTD 240

Query: 241 MKLTL-SALEALKEIRGGSSTIDVFS 250
           MK T+ +AL AL+EIR GSST+ VFS
Sbjct: 241 MKSTVTAALRALREIRSGSSTVSVFS 263

BLAST of Carg00449 vs. ExPASy TrEMBL
Match: A0A6J1FTG2 (SPX domain-containing protein 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446680 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.0e-129
Identity = 244/254 (96.06%), Postives = 248/254 (97.64%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKFWKILCILIESTLPEWR+EFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL
Sbjct: 1   MKFWKILCILIESTLPEWRDEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60

Query: 61  LEEELDKFNRFFVSKEEYYVIKWS---ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120
           LEEELDKFN+FFVSKEEYYVIKWS   ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY
Sbjct: 61  LEEELDKFNQFFVSKEEYYVIKWSLLQERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120

Query: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180
           SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK
Sbjct: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180

Query: 181 DLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKE 240
           DLSSA+ETVNEEG   GSEAKTASESKEKVLNIPEDLEEIE+MESMYMKLTLSALEALKE
Sbjct: 181 DLSSASETVNEEGIMGGSEAKTASESKEKVLNIPEDLEEIEHMESMYMKLTLSALEALKE 240

Query: 241 IRGGSSTIDVFSWL 252
           IRGGSSTIDVFSWL
Sbjct: 241 IRGGSSTIDVFSWL 254

BLAST of Carg00449 vs. ExPASy TrEMBL
Match: A0A6J1ISJ6 (SPX domain-containing protein 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480222 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.0e-129
Identity = 244/254 (96.06%), Postives = 248/254 (97.64%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKFWKILCILIESTLPEWR+EFISYKKLKKQLKLMYPDKDG PKRLKFDGEASMEVFLNL
Sbjct: 1   MKFWKILCILIESTLPEWRDEFISYKKLKKQLKLMYPDKDGKPKRLKFDGEASMEVFLNL 60

Query: 61  LEEELDKFNRFFVSKEEYYVIKWS---ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120
           LEEELDKFN+FFVSKEEYYVIKWS   ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY
Sbjct: 61  LEEELDKFNQFFVSKEEYYVIKWSLLQERVAEIGDSDERLMKVGRDIVDFHGEMVLLENY 120

Query: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180
           SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK
Sbjct: 121 SALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKK 180

Query: 181 DLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKE 240
           DLSSAAETVNEEGR+ GSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSAL+ALKE
Sbjct: 181 DLSSAAETVNEEGRRGGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALKALKE 240

Query: 241 IRGGSSTIDVFSWL 252
           IRGGSSTID FSWL
Sbjct: 241 IRGGSSTIDDFSWL 254

BLAST of Carg00449 vs. ExPASy TrEMBL
Match: A0A6J1J0N4 (SPX domain-containing protein 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480222 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.3e-121
Identity = 232/251 (92.43%), Postives = 238/251 (94.82%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKFWKILCILIESTLPEWR+EFISYKKLKKQLKLMYPDKDG PKRLKFDGEASMEVFLNL
Sbjct: 1   MKFWKILCILIESTLPEWRDEFISYKKLKKQLKLMYPDKDGKPKRLKFDGEASMEVFLNL 60

Query: 61  LEEELDKFNRFFVSKEEYYVIKWSERVAEIGDSDERLMKVGRDIVDFHGEMVLLENYSAL 120
           LEEELDKFN+FF+ +         ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENYSAL
Sbjct: 61  LEEELDKFNQFFLLQ---------ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENYSAL 120

Query: 121 NYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLS 180
           NYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLS
Sbjct: 121 NYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLS 180

Query: 181 SAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKEIRG 240
           SAAETVNEEGR+ GSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSAL+ALKEIRG
Sbjct: 181 SAAETVNEEGRRGGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALKALKEIRG 240

Query: 241 GSSTIDVFSWL 252
           GSSTID FSWL
Sbjct: 241 GSSTIDDFSWL 242

BLAST of Carg00449 vs. ExPASy TrEMBL
Match: A0A6J1FKX6 (SPX domain-containing protein 2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446680 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.3e-121
Identity = 232/251 (92.43%), Postives = 238/251 (94.82%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKFWKILCILIESTLPEWR+EFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL
Sbjct: 1   MKFWKILCILIESTLPEWRDEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60

Query: 61  LEEELDKFNRFFVSKEEYYVIKWSERVAEIGDSDERLMKVGRDIVDFHGEMVLLENYSAL 120
           LEEELDKFN+FF+ +         ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENYSAL
Sbjct: 61  LEEELDKFNQFFLLQ---------ERVAEIGDSDERLMKVGRDIVDFHGEMVLLENYSAL 120

Query: 121 NYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLS 180
           NYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLS
Sbjct: 121 NYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLS 180

Query: 181 SAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKEIRG 240
           SA+ETVNEEG   GSEAKTASESKEKVLNIPEDLEEIE+MESMYMKLTLSALEALKEIRG
Sbjct: 181 SASETVNEEGIMGGSEAKTASESKEKVLNIPEDLEEIEHMESMYMKLTLSALEALKEIRG 240

Query: 241 GSSTIDVFSWL 252
           GSSTIDVFSWL
Sbjct: 241 GSSTIDVFSWL 242

BLAST of Carg00449 vs. ExPASy TrEMBL
Match: A0A6J1J0I4 (SPX domain-containing protein 2-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111480222 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.0e-109
Identity = 211/220 (95.91%), Postives = 214/220 (97.27%), Query Frame = 0

Query: 35  MYPDKDGNPKRLKFDGEASMEVFLNLLEEELDKFNRFFVSKEEYYVIKWS---ERVAEIG 94
           MYPDKDG PKRLKFDGEASMEVFLNLLEEELDKFN+FFVSKEEYYVIKWS   ERVAEIG
Sbjct: 1   MYPDKDGKPKRLKFDGEASMEVFLNLLEEELDKFNQFFVSKEEYYVIKWSLLQERVAEIG 60

Query: 95  DSDERLMKVGRDIVDFHGEMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQ 154
           DSDERLMKVGRDIVDFHGEMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQ
Sbjct: 61  DSDERLMKVGRDIVDFHGEMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQ 120

Query: 155 PFYSTDVLGQLLKECEMVLDLLFFKKDLSSAAETVNEEGRQVGSEAKTASESKEKVLNIP 214
           PFYSTDVLGQLLKECEMVLDLLFFKKDLSSAAETVNEEGR+ GSEAKTASESKEKVLNIP
Sbjct: 121 PFYSTDVLGQLLKECEMVLDLLFFKKDLSSAAETVNEEGRRGGSEAKTASESKEKVLNIP 180

Query: 215 EDLEEIEYMESMYMKLTLSALEALKEIRGGSSTIDVFSWL 252
           EDLEEIEYMESMYMKLTLSAL+ALKEIRGGSSTID FSWL
Sbjct: 181 EDLEEIEYMESMYMKLTLSALKALKEIRGGSSTIDDFSWL 220

BLAST of Carg00449 vs. TAIR 10
Match: AT2G26660.1 (SPX domain gene 2 )

HSP 1 Score: 247.3 bits (630), Expect = 1.3e-65
Identity = 140/265 (52.83%), Postives = 186/265 (70.19%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGN--PKRLKFD--------- 60
           MKF K L   IE TLPEWR++F+SYK+LKK+LKLM P    N   KR + D         
Sbjct: 1   MKFGKSLSNQIEETLPEWRDKFLSYKELKKKLKLMEPRSVENRPNKRSRSDSNSVDTDPT 60

Query: 61  -GEASMEV-FLNLLEEELDKFNRFFVSKEEYYVIKWSE---RVAEIGDSDERLMKVGRDI 120
            G    E+ F++LLE+EL+KFN FFV +EE Y+I+  E   +VA+  +S+E ++ + ++I
Sbjct: 61  VGMTKEELDFISLLEDELEKFNSFFVEQEEEYIIRLKELKDQVAKAKNSNEEMINIKKEI 120

Query: 121 VDFHGEMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLK 180
           VDFHGEMVLL NYSALNYTGLAKILKKYDKR+G LIR+PFI+KVL++PF++TD+L   +K
Sbjct: 121 VDFHGEMVLLMNYSALNYTGLAKILKKYDKRTGALIRLPFIQKVLQEPFFTTDLLNTFVK 180

Query: 181 ECEMVLDLLFFKKDLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMY 240
           ECE +LD LF     S+ +  ++EEG    S          ++L +P++L EIEYMES+Y
Sbjct: 181 ECEAMLDRLF----PSNKSRNLDEEGEPTTSGMVKTGTDDSELLRVPKELSEIEYMESLY 240

Query: 241 MKLTLSALEALKEIRGGSSTIDVFS 250
           MK T+SAL+ LKEIR GSST+ VFS
Sbjct: 241 MKSTVSALKVLKEIRSGSSTVSVFS 261

BLAST of Carg00449 vs. TAIR 10
Match: AT5G20150.1 (SPX domain gene 1 )

HSP 1 Score: 230.3 bits (586), Expect = 1.7e-60
Identity = 132/258 (51.16%), Postives = 180/258 (69.77%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNP-KRLKFD----GEASME 60
           MKF K L   IE TLPEW+++F+SYK+LKK+LKL+       P KRL+ D    G +  E
Sbjct: 1   MKFGKSLSNQIEQTLPEWQDKFLSYKELKKRLKLIGSKTADRPVKRLRLDEFSVGISKEE 60

Query: 61  V-FLNLLEEELDKFNRFFVSKEEYYVIKWSE---RVAEIGDSDERLMKVGRDIVDFHGEM 120
           + F+ LLE+EL+KFN FFV KEE Y+I+  E   R+A+  DS E+++K+ ++IVDFHGEM
Sbjct: 61  INFIQLLEDELEKFNNFFVEKEEEYIIRLKEFRDRIAKAKDSMEKMIKIRKEIVDFHGEM 120

Query: 121 VLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLD 180
           VLLENYSALNYTGL KILKKYDKR+G+L+R+PFI+KVL+QPFY+TD+L +L+KE E +LD
Sbjct: 121 VLLENYSALNYTGLVKILKKYDKRTGDLMRLPFIQKVLQQPFYTTDLLFKLVKESEAMLD 180

Query: 181 LLFFKKDLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTLSA 240
            +F                         A+E++ ++  I  +L E ++MES++MK T++A
Sbjct: 181 QIF------------------------PANETESEI--IQAELSEHKFMESLHMKSTIAA 232

Query: 241 LEALKEIRGGSSTIDVFS 250
           L  LKEIR GSST+ VFS
Sbjct: 241 LRVLKEIRSGSSTVSVFS 232

BLAST of Carg00449 vs. TAIR 10
Match: AT5G15330.1 (SPX domain gene 4 )

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-36
Identity = 108/292 (36.99%), Postives = 154/292 (52.74%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYP---------DKDGNPKRLKF--- 60
           MKF K     +E TLPEWR++F+ YK LKK LK  YP         + D N  R  F   
Sbjct: 1   MKFGKEFRTHLEETLPEWRDKFLCYKPLKKLLK-YYPYYSADFGPANSDHNDSRPVFADT 60

Query: 61  --------DG---------EASMEVFLNLLEEELDKFNRFFVSKEEYYVIK---WSERVA 120
                   DG         E     F+ +L +EL+KFN F+V KEE +VI+     ER+ 
Sbjct: 61  TNISSAADDGGVVPGVRPSEDLQGSFVRILNDELEKFNDFYVDKEEDFVIRLQELKERIE 120

Query: 121 EIGDSD----------ERLMKVGRDIVDFHGEMVLLENYSALNYTGLAKILKKYDKRSGE 180
           ++ + +          E +M + RD+V  HGEMVLL+NYS+LN+ GL KILKKYDKR+G 
Sbjct: 121 QVKEKNGEFASESEFSEEMMDIRRDLVTIHGEMVLLKNYSSLNFAGLVKILKKYDKRTGG 180

Query: 181 LIRVPFIKKVLRQPFYSTDVLGQLLKECEMVLDLLFFKKDLSSAAETVNEEGRQVGSEAK 240
           L+R+PF + VL QPF++T+ L +L++ECE  L+LLF      S AE V         +A 
Sbjct: 181 LLRLPFTQLVLHQPFFTTEPLTRLVRECEANLELLF-----PSEAEVVESSS---AVQAH 240

Query: 241 TASESKEKVLNIPEDLEEIEYMESMYMKLTLSALEALKEIRGGSSTIDVFSW 251
           ++S          E    +        K TL+A+ A++ ++  SST +  S+
Sbjct: 241 SSSHQHNSPRISAETSSTLGNENLDIYKSTLAAMRAIRGLQKASSTYNPLSF 283

BLAST of Carg00449 vs. TAIR 10
Match: AT2G45130.1 (SPX domain gene 3 )

HSP 1 Score: 146.4 bits (368), Expect = 3.2e-35
Identity = 99/260 (38.08%), Postives = 142/260 (54.62%), Query Frame = 0

Query: 1   MKFWKILCILIESTLPEWRNEFISYKKLKKQLKLMYPDKDGNPKRLKFDGEASMEVFLNL 60
           MKF K +   I+ +LPEWR++F+ YK+LK  +    P +                +F+ L
Sbjct: 1   MKFGKRIKEQIQESLPEWRDKFLRYKELKNLISSPAPVE---------------SIFVGL 60

Query: 61  LEEELDKFNRFFVSKEEYYVIKWSE-------RVAEIGDSD----ERLMKVGRDIVDFHG 120
           L  E+DKFN FFV +EE ++I   E        V + G +D    E + ++ +DIV+FHG
Sbjct: 61  LNAEIDKFNAFFVEQEEDFIIHHKELQYRIQRLVEKCGHNDEMSRENISEIRKDIVNFHG 120

Query: 121 EMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDVLGQLLKECEMV 180
           EMVLL NYS +NYTGLAKILKKYDKR+   +R PFI+KVL QPF+ TD++ +L++E E  
Sbjct: 121 EMVLLVNYSNINYTGLAKILKKYDKRTRGGLRSPFIQKVLHQPFFKTDLVSRLVREWETT 180

Query: 181 LDLLFFKKDLSSAAETVNEEGRQVGSEAKTASESKEKVLNIPEDLEEIEYMESMYMKLTL 240
           +D +    D    AE    E       A T++ + E +                  + T+
Sbjct: 181 MDAV----DPVKVAEAEGYE----RCAAVTSAAAGEGI-----------------FRNTV 220

Query: 241 SALEALKEIRGGSSTIDVFS 250
           +AL  +KE+R GSST   FS
Sbjct: 241 AALLTMKEMRRGSSTYSAFS 220

BLAST of Carg00449 vs. TAIR 10
Match: AT3G23430.1 (phosphate 1 )

HSP 1 Score: 49.7 bits (117), Expect = 4.0e-06
Identity = 27/84 (32.14%), Postives = 44/84 (52.38%), Query Frame = 0

Query: 99  KVGRDIVDFHGEMVLLENYSALNYTGLAKILKKYDKRSGELIRVPFIKKVLRQPFYSTDV 158
           K+    V+ +  + LL+ YS+LN     KI+KK+DK +G+     ++K V R  F S+D 
Sbjct: 287 KIRSAFVELYRGLGLLKTYSSLNMIAFTKIMKKFDKVAGQNASSTYLKVVKRSQFISSDK 346

Query: 159 LGQLLKECEMVLDLLFFKKDLSSA 183
           + +L+ E E +    F   D   A
Sbjct: 347 VVRLMDEVESIFTKHFANNDRKKA 370