Carg00375 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00375
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	D-amino-acid transaminase, chloroplastic
Location	Carg_Chr04: 6889228 .. 6891855 (+)
RNA-Seq Expression	Carg00375
Synteny	Carg00375

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTGAACAAAACATTTTCTCAAATTATCCTGAACATTAATCTCCGATGAATTCTCTGCAGTTTCTTCTCAAACCCATTCCTCAGTCACCGATCAGCGGTGCAGAACTCACTGCCCATTCTCAAAGGCCGCTCGACAAATCCTGCAATTCTCGGCAGTTGTCTTTTAGAAGATTCAGAATTAGGTGTTCAATTGCTGGTTAGTTTTTTTTTTCAAATTCGTCGCGAGAATGTTTGATAATTGGTTCCACTGGAGCCTTGTTTATCTAAGCAAGTTTGTTTGAAACTGTTTAATAGTTGACTCGCTTTTCATAATCTGGTTGTTTTACGACACTGTTCGGGCTTTTGCGATCCTGATTCACTTTTCATGATCTGGTTGTTTACGATACTGTTCAGGCTTTTGCGATCCTAACTTTTTTTTCAATTTTCTCTATCCGTTCGATCTGAAATCTGAATGATCAAAGCAACTGCTCGTCTTGTCGCAGATACCGTTGATCGTATTTCTGATGCTCCAGTGCTTGGTAGTTCACAGGTCAACTCTTGTTGTGTATTGTAATCTGTTTGTGTGTTGAAATAAACTACAAATTTTAGTGTTCTATGCGTGGAAGGTGATAATTGTATAGCTTTACTGATTGTTATTTCGATCCATGTGTTTATTGAAGGTGCTTGAAAGATTAAGGGCAAGCCGAGAGAATCAAGAAAACCAGCCACAATTCCTTGCAATGTATTCCAGCGTTGTTGGGGGAATTACGACAGATCCAGCTGTTATGGTAATTCCAATTGATGACCACATGGTTCATCGGGGACATGGCGTTTTTGATACTGCTGCCATAGTGGATGGGTATGTGAGAATTTCATACAGTTTCACCAAGATTATGGTGAAATCCCACAATTCCACGTTGGTCAGGAGGAGAACGAAACACCCTTTGTAAGGGTGTGGAAACCTTTTCCTAGTAGACCTGTTTTAAAGCCTTTGAGGGGAAACCCGAAAGGGAAAGCCCAAAGACGACAAATATATGCTAGTGGTGGATTTGGGCCGTTACAATTATGAATCTGATATCGATTTCCCGAGACCTGCATAATTAGCACTGTAAATTGACAGGCTCTTATGAACATTTGAATTCATAACTAAATTGATACATATTTTTCTTCAATGGAAGTAGGAAATGCATACTTTCATTTCATGATTCTGTCAGTGTTGAGTTACTGTATAAAATGGTTTTGACATCTCAGGCATCTCTATGAGTTGGACCAGCACCTCGACCGCATTTTAAGATCAGCATCCATGGCAAAAATCAACCTCCCAGTTTCATATGATCGAGAGAAGATTAAAAATATACTCATAAGAACTGCCAGTGCTTCTAAGTGTAGGAATGGACAGCTTCGATATTGGCTCTCTGCAGGACCTGGAAATTTTCTACTATCTACTTCTGGGTTGCATCAGCCAGCTCTTTATGCAATTGTAATTCAAGGTAAGCCGCCATCTCCTCCAAAAGGCATCAAAGTCATAACTTCATCAATCCCAATGAAACCACCTCAATTTGCAACTATGAAGAGTGTAAATTACCTTCCAAATGTTCTTTCAAATATGGAAGCAGAAGAAAAGGGTGCTTATACAGCCATTTGGTTGGATAGTGAGGGGTTCATTGCTGAAGGACCTAGTATGAACGTTGGTTTCATTACAAAGGATAAGGAGTTTCTGATGCCTCACTTTGACAAAATTTTAAGCGGCTGCACAGCTAAGAGAATCATAACTCTCGCTGAGCGGCTGGTGACAGAGGGTAGGCTTCGGAGTATAAGATTTGAAAATTTAACCATGGAGGAAGGCAAGAAGGCAGAGGAAATGGTGCTTATTGGGAGTGGAGTTCTTGTTTCCCCTGTACTGCAGTGGGATGAGCAGATTATTGGTGATGGTAAGTACCCTCTATAATCCTTAATTCATAAATAAGATGTTTTTGTAGTTCTGAATCATGTTGTGATTGGCTGGATGAATTTCTTCTAACATGTTTAAAATTCCATTCAAAGTACTTCAATTCAAACAAACATGGAGAGTGTTTTTGTAAGCACTTAGAAAGTCGTTCTAAACCGATTCTAAAATTAGTTTTGTAGAATGATGATACCATGACATGAAATAGGAACACACGTAGTTTTCTTCCCACCGCAACCACATCTAAGAAAGGCACCATTGTAATATTTCTGATGGTGGGGATGAGTGTTCTTATTATAGAACAAGAACATGAAAGCATGGAAACTTGTTTTTATCTGACAAATTAAGAATGTTTTGGTTTTGCTCCACAGGAAGAGAAGGTCCGTTGGCTCGAGCGATTGTAGATCTTATTATCGAAGACATGAAATTCGGCCCTCCAACCGTTCGAATACCAATTCCATATGAATAACTCAGGTCAACCTATCATCTCTGTCTCATGATTTAATGAGACACTCAAAAATGCATTTGGAACATCTAATTGAAACTGAGGTCAATCTATGAATGCAGCTAGTACAAGCAATGTATATGTGGACGGGAATAAGGATAACAGTTTCACGAGTGATATCCAAACTGTGAAAATTTTAGGAAGTGTTTTAATAAATACTCTTAAAAAGTATATTTAGAATAATTTTTTTTTAATAAT

mRNA sequence

AATTGAACAAAACATTTTCTCAAATTATCCTGAACATTAATCTCCGATGAATTCTCTGCAGTTTCTTCTCAAACCCATTCCTCAGTCACCGATCAGCGGTGCAGAACTCACTGCCCATTCTCAAAGGCCGCTCGACAAATCCTGCAATTCTCGGCAGTTGTCTTTTAGAAGATTCAGAATTAGGTGTTCAATTGCTGATACCGTTGATCGTATTTCTGATGCTCCAGTGCTTGGTAGTTCACAGGTGCTTGAAAGATTAAGGGCAAGCCGAGAGAATCAAGAAAACCAGCCACAATTCCTTGCAATGTATTCCAGCGTTGTTGGGGGAATTACGACAGATCCAGCTGTTATGGTAATTCCAATTGATGACCACATGGTTCATCGGGGACATGGCGTTTTTGATACTGCTGCCATAGTGGATGGGCATCTCTATGAGTTGGACCAGCACCTCGACCGCATTTTAAGATCAGCATCCATGGCAAAAATCAACCTCCCAGTTTCATATGATCGAGAGAAGATTAAAAATATACTCATAAGAACTGCCAGTGCTTCTAAGTGTAGGAATGGACAGCTTCGATATTGGCTCTCTGCAGGACCTGGAAATTTTCTACTATCTACTTCTGGGTTGCATCAGCCAGCTCTTTATGCAATTGTAATTCAAGGTAAGCCGCCATCTCCTCCAAAAGGCATCAAAGTCATAACTTCATCAATCCCAATGAAACCACCTCAATTTGCAACTATGAAGAGTGTAAATTACCTTCCAAATGTTCTTTCAAATATGGAAGCAGAAGAAAAGGGTGCTTATACAGCCATTTGGTTGGATAGTGAGGGGTTCATTGCTGAAGGACCTAGTATGAACGTTGGTTTCATTACAAAGGATAAGGAGTTTCTGATGCCTCACTTTGACAAAATTTTAAGCGGCTGCACAGCTAAGAGAATCATAACTCTCGCTGAGCGGCTGGTGACAGAGGGTAGGCTTCGGAGTATAAGATTTGAAAATTTAACCATGGAGGAAGGCAAGAAGGCAGAGGAAATGGTGCTTATTGGGAGTGGAGTTCTTGTTTCCCCTGTACTGCAGTGGGATGAGCAGATTATTGGTGATGGAAGAGAAGGTCCGTTGGCTCGAGCGATTGTAGATCTTATTATCGAAGACATGAAATTCGGCCCTCCAACCGTTCGAATACCAATTCCATATGAATAACTCAGGTCAACCTATCATCTCTGTCTCATGATTTAATGAGACACTCAAAAATGCATTTGGAACATCTAATTGAAACTGAGGTCAATCTATGAATGCAGCTAGTACAAGCAATGTATATGTGGACGGGAATAAGGATAACAGTTTCACGAGTGATATCCAAACTGTGAAAATTTTAGGAAGTGTTTTAATAAATACTCTTAAAAAGTATATTTAGAATAATTTTTTTTTAATAAT

Coding sequence (CDS)

ATGAATTCTCTGCAGTTTCTTCTCAAACCCATTCCTCAGTCACCGATCAGCGGTGCAGAACTCACTGCCCATTCTCAAAGGCCGCTCGACAAATCCTGCAATTCTCGGCAGTTGTCTTTTAGAAGATTCAGAATTAGGTGTTCAATTGCTGATACCGTTGATCGTATTTCTGATGCTCCAGTGCTTGGTAGTTCACAGGTGCTTGAAAGATTAAGGGCAAGCCGAGAGAATCAAGAAAACCAGCCACAATTCCTTGCAATGTATTCCAGCGTTGTTGGGGGAATTACGACAGATCCAGCTGTTATGGTAATTCCAATTGATGACCACATGGTTCATCGGGGACATGGCGTTTTTGATACTGCTGCCATAGTGGATGGGCATCTCTATGAGTTGGACCAGCACCTCGACCGCATTTTAAGATCAGCATCCATGGCAAAAATCAACCTCCCAGTTTCATATGATCGAGAGAAGATTAAAAATATACTCATAAGAACTGCCAGTGCTTCTAAGTGTAGGAATGGACAGCTTCGATATTGGCTCTCTGCAGGACCTGGAAATTTTCTACTATCTACTTCTGGGTTGCATCAGCCAGCTCTTTATGCAATTGTAATTCAAGGTAAGCCGCCATCTCCTCCAAAAGGCATCAAAGTCATAACTTCATCAATCCCAATGAAACCACCTCAATTTGCAACTATGAAGAGTGTAAATTACCTTCCAAATGTTCTTTCAAATATGGAAGCAGAAGAAAAGGGTGCTTATACAGCCATTTGGTTGGATAGTGAGGGGTTCATTGCTGAAGGACCTAGTATGAACGTTGGTTTCATTACAAAGGATAAGGAGTTTCTGATGCCTCACTTTGACAAAATTTTAAGCGGCTGCACAGCTAAGAGAATCATAACTCTCGCTGAGCGGCTGGTGACAGAGGGTAGGCTTCGGAGTATAAGATTTGAAAATTTAACCATGGAGGAAGGCAAGAAGGCAGAGGAAATGGTGCTTATTGGGAGTGGAGTTCTTGTTTCCCCTGTACTGCAGTGGGATGAGCAGATTATTGGTGATGGAAGAGAAGGTCCGTTGGCTCGAGCGATTGTAGATCTTATTATCGAAGACATGAAATTCGGCCCTCCAACCGTTCGAATACCAATTCCATATGAATAA

Protein sequence

MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIITLAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLARAIVDLIIEDMKFGPPTVRIPIPYE

Homology

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BLAST of Carg00375 vs. NCBI nr
Match: KAG7031718.1 (D-amino-acid transaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 762.3 bits (1967), Expect = 2.0e-216
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60
           MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP
Sbjct: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 AIVDLIIEDMKFGPPTVRIPIPYE 385
           AIVDLIIEDMKFGPPTVRIPIPYE
Sbjct: 361 AIVDLIIEDMKFGPPTVRIPIPYE 384

[Close]

BLAST of Carg00375 vs. NCBI nr
Match: XP_022940281.1 (D-amino-acid transaminase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 756.9 bits (1953), Expect = 8.4e-215
Identity = 381/384 (99.22%), Postives = 382/384 (99.48%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60
           MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP
Sbjct: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLV EGRLR IRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVKEGRLRGIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 AIVDLIIEDMKFGPPTVRIPIPYE 385
           AIVDLIIEDMKFGPPTVRIPIPY+
Sbjct: 361 AIVDLIIEDMKFGPPTVRIPIPYK 384

[Close]

BLAST of Carg00375 vs. NCBI nr
Match: XP_023521525.1 (D-amino-acid transaminase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 737.6 bits (1903), Expect = 5.3e-209
Identity = 373/384 (97.14%), Postives = 376/384 (97.92%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60
           MNSLQ LLKPIPQSPIS AELTAHSQRPL+ SCNSRQLSFRRFRIRCSIADTVDR  DAP
Sbjct: 1   MNSLQSLLKPIPQSPISDAELTAHSQRPLNNSCNSRQLSFRRFRIRCSIADTVDRTFDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQ ALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQSALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 AIVDLIIEDMKFGPPTVRIPIPYE 385
           A+VDLIIEDMK GPPTVRI +PYE
Sbjct: 361 ALVDLIIEDMKSGPPTVRIRVPYE 384

[Close]

BLAST of Carg00375 vs. NCBI nr
Match: XP_022981012.1 (D-amino-acid transaminase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 736.5 bits (1900), Expect = 1.2e-208
Identity = 373/384 (97.14%), Postives = 376/384 (97.92%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60
           MN+LQ LLKPIPQSPISGAELTAHSQRPL KSCNSRQ SFRRFRIRCSIADTVDR SDAP
Sbjct: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLV EGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 AIVDLIIEDMKFGPPTVRIPIPYE 385
           A+VDLIIEDMK GP TVRIP+ YE
Sbjct: 361 ALVDLIIEDMKSGPLTVRIPVLYE 384

[Close]

BLAST of Carg00375 vs. NCBI nr
Match: KAG6608083.1 (D-amino-acid transaminase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 684.1 bits (1764), Expect = 6.9e-193
Identity = 354/384 (92.19%), Postives = 355/384 (92.45%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60
           MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP
Sbjct: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK          
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK---------- 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
                            + YAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 ----------------SSFYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLVTEGRLR IRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVTEGRLRGIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 358

Query: 361 AIVDLIIEDMKFGPPTVRIPIPYE 385
           AIVDLIIEDMKFGPPTVRIPIPYE
Sbjct: 361 AIVDLIIEDMKFGPPTVRIPIPYE 358

[Close]

BLAST of Carg00375 vs. ExPASy Swiss-Prot
Match: Q8L493 (D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT PE=1 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 5.1e-122
Identity = 220/349 (63.04%), Postives = 281/349 (80.52%), Query Frame = 0


Query: 36  RQLSFRRFRIRCSIADTVDRISDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGI 95
           R+L+ RR  + CS  D+  +  + PVL S +V ERL+ +R  Q    QFLAMYSSVV GI
Sbjct: 33  RRLTRRRTILMCS--DSSSQSWNVPVLSSYEVGERLKLARGGQ----QFLAMYSSVVDGI 92

Query: 96  TTDPAVMVIPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDR 155
           TTDPA MV+P+DDHMVHRGHGVFDTA I++G+LYELDQHLDRILRSASMAKI LP  +DR
Sbjct: 93  TTDPAAMVLPLDDHMVHRGHGVFDTALIINGYLYELDQHLDRILRSASMAKIPLP--FDR 152

Query: 156 EKIKNILIRTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGI 215
           E IK ILI+T S S CR+G LRYWLSAGPG+FLLS S   +P LYAIVI+      P G+
Sbjct: 153 ETIKRILIQTVSVSGCRDGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTNFAINPIGV 212

Query: 216 KVITSSIPMKPPQFATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFI 275
           KV+TSSIP+KPP+FAT+KSVNYLPNVLS MEAE KGAY  IW+  +GFIAEGP+MNV F+
Sbjct: 213 KVVTSSIPIKPPEFATVKSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEGPNMNVAFV 272

Query: 276 TK-DKEFLMPHFDKILSGCTAKRIITLAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIG 335
               KE +MP FD +LSGCTAKR +TLAE+LV++G L++++  ++T+E+GKKA+EM+LIG
Sbjct: 273 VNGGKELVMPRFDNVLSGCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKKADEMMLIG 332

Query: 336 SGVLVSPVLQWDEQIIGDGREGPLARAIVDLIIEDMKFGPPTVRIPIPY 384
           SG+ + PV+QWDE+ IG+G+EGP+A+A++DL++EDM+ GPP+VR+ +PY
Sbjct: 333 SGIPIRPVIQWDEEFIGEGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]

BLAST of Carg00375 vs. ExPASy Swiss-Prot
Match: Q58414 (Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=ilvE PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 4.6e-22
Identity = 70/261 (26.82%), Postives = 130/261 (49.81%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILI 163
           + + DH +  G GVF+     DG ++ L +H+DR+  SA    I++P++  +E++ ++++
Sbjct: 17  VSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLT--KEEMIDVVL 76

Query: 164 RTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIP 223
            T   +  R+  +R  ++ G G+  L      +P ++ I I   P     GI+ IT S+ 
Sbjct: 77  ETLRVNNLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGEDGIRAITVSVR 136

Query: 224 MKPPQF--ATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEF 283
             P       +KS+NYL +VL+ ++A   G   A  LD +GF+ EG   N+  +      
Sbjct: 137 RLPVDVLNPAVKSLNYLNSVLAKIQANYAGVDEAFLLDDKGFVVEGTGDNIFIVKNGVLK 196

Query: 284 LMPHFDKILSGCTAKRIITLAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSP 343
             P +  IL G T   +I LA+    E     +  E LT+ +   A+E+ + G+   + P
Sbjct: 197 TPPVYQSILKGITRDVVIKLAKEEGIE-----VVEEPLTLHDLYTADELFITGTAAEIVP 256

Query: 344 VLQWDEQIIGDGREGPLARAI 363
           V + D ++I + + G + + +
Sbjct: 257 VFEIDGRVINNKQVGEITKKL 270

[Close]

BLAST of Carg00375 vs. ExPASy Swiss-Prot
Match: O29329 (Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=ilvE PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.3e-21
Identity = 76/274 (27.74%), Postives = 136/274 (49.64%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILI 163
           + I DH    G GVF+     +G ++ L +H+DR+  SA    + +P++  +E+   I++
Sbjct: 18  VSIFDHGFLYGDGVFEGIRAYNGRVFRLKEHIDRLYDSAKAIDLEIPIT--KEEFMEIIL 77

Query: 164 RTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSP------PKGIKV 223
            T   +  R+  +R  ++ G G+  L       P   +I++  KP          KG+  
Sbjct: 78  ETLRKNNLRDAYIRPIVTRGIGDLGLDPRKCQNP---SIIVITKPWGKLYGDLYEKGLTA 137

Query: 224 IT-----SSIPMKPPQFATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNV 283
           IT     +S    PP    +KS+NYL N+L+ +EA  KG   AI+LD  G+++EG   N+
Sbjct: 138 ITVAVRRNSFDALPP---NIKSLNYLNNILAKIEANAKGGDEAIFLDRNGYVSEGSGDNI 197

Query: 284 GFITKDKEFLMPHFDKILSGCTAKRIITLAERLVTEGRLRSIRFE--NLTMEEGKKAEEM 343
            F+ K+     P     L G T + +I +  RL        I F+  N+ + +   A+E+
Sbjct: 198 -FVVKNGAITTPPTINNLRGITREAVIEIINRL-------GIPFKETNIGLYDLYTADEV 257

Query: 344 VLIGSGVLVSPVLQWDEQIIGDGREGPLARAIVD 365
            + G+   ++P++  D + IGDG+ G + R +++
Sbjct: 258 FVTGTAAEIAPIVVIDGRKIGDGKPGEITRKLME 275

[Close]

BLAST of Carg00375 vs. ExPASy Swiss-Prot
Match: Q8W0Z7 (Branched-chain-amino-acid aminotransferase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At3g05190 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 9.0e-18
Identity = 84/294 (28.57%), Postives = 142/294 (48.30%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE++K  +
Sbjct: 278 VSVFDSVVQGGDSVWEGLRIYKGKIFKLEEHLDRLFDSAKALAFDNVPA---REEVKEAI 337

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPA------LYAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 338 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAYNRYGCTLIVLAEWKPPVYDNEG 397

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+    A  AI LD +G+++E  + N
Sbjct: 398 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNTNAADAIMLDKDGYVSETNATN 457

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLA--ERLVTEGRLRSIRFENLTMEEGKKAEE 343
           + F+ K    L PH D  L G T   ++ L   E  + E R        +++ E   A E
Sbjct: 458 I-FMVKKGCVLTPHADYCLPGITRATVMELVVKENFILEER-------RISLSEFHTANE 517

Query: 344 MVLIGSGVLVSPVLQWDEQIIGDGREGPLARAIVDL---IIEDMKFGPPTVRIP 381
           +   G+   +SPV++ D ++IGDG+ GP+ R + +    + ED     PT + P
Sbjct: 518 VWTTGTMGELSPVVKIDGRVIGDGKVGPVTRTLQNAYKKLTEDSGVPIPTYQEP 555

[Close]

BLAST of Carg00375 vs. ExPASy Swiss-Prot
Match: Q9ASR4 (Branched-chain-amino-acid aminotransferase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g27410 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.5e-17
Identity = 79/273 (28.94%), Postives = 135/273 (49.45%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE+IK  +
Sbjct: 275 VSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT---REEIKEAI 334

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPAL------YAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 335 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAFNRYGCTLIVLAEWKPPVYDNDG 394

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+       AI LD +GF++E  + N
Sbjct: 395 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETNATN 454

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLA--ERLVTEGRLRSIRFENLTMEEGKKAEE 343
           +  + KD+  L PH D  L G T   ++ L   E  + E R        +++ E   A+E
Sbjct: 455 IFMVKKDR-VLTPHADYCLPGITRATVMELVVKENFILEER-------RISLSEFHTADE 514

Query: 344 MVLIGSGVLVSPVLQWDEQIIGDGREGPLARAI 363
           +   G+   +SPV++ D ++IG+G+ GP+ R +
Sbjct: 515 VWTTGTMGELSPVVKIDGRVIGEGKVGPVTRRL 531

[Close]

BLAST of Carg00375 vs. ExPASy TrEMBL
Match: A0A6J1FI15 (D-amino-acid transaminase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445949 PE=4 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 4.1e-215
Identity = 381/384 (99.22%), Postives = 382/384 (99.48%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60
           MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP
Sbjct: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLV EGRLR IRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVKEGRLRGIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 AIVDLIIEDMKFGPPTVRIPIPYE 385
           AIVDLIIEDMKFGPPTVRIPIPY+
Sbjct: 361 AIVDLIIEDMKFGPPTVRIPIPYK 384

[Close]

BLAST of Carg00375 vs. ExPASy TrEMBL
Match: A0A6J1IST6 (D-amino-acid transaminase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111480287 PE=4 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 5.7e-209
Identity = 373/384 (97.14%), Postives = 376/384 (97.92%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKSCNSRQLSFRRFRIRCSIADTVDRISDAP 60
           MN+LQ LLKPIPQSPISGAELTAHSQRPL KSCNSRQ SFRRFRIRCSIADTVDR SDAP
Sbjct: 1   MNALQSLLKPIPQSPISGAELTAHSQRPLGKSCNSRQWSFRRFRIRCSIADTVDRSSDAP 60

Query: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120
           VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT
Sbjct: 61  VLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHMVHRGHGVFDT 120

Query: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180
           AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL
Sbjct: 121 AAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASKCRNGQLRYWL 180

Query: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240
           SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN
Sbjct: 181 SAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFATMKSVNYLPN 240

Query: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300
           VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT
Sbjct: 241 VLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKILSGCTAKRIIT 300

Query: 301 LAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360
           LAERLV EGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR
Sbjct: 301 LAERLVKEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQIIGDGREGPLAR 360

Query: 361 AIVDLIIEDMKFGPPTVRIPIPYE 385
           A+VDLIIEDMK GP TVRIP+ YE
Sbjct: 361 ALVDLIIEDMKSGPLTVRIPVLYE 384

[Close]

BLAST of Carg00375 vs. ExPASy TrEMBL
Match: A0A6J1CNI4 (D-amino-acid transaminase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111012818 PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.7e-171
Identity = 313/393 (79.64%), Postives = 339/393 (86.26%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPL----------DKSCNSRQLSFRRFRIRCSIA 60
           M SLQ LLKP+    IS  E     QRPL           +S NSRQLS R FRIR SIA
Sbjct: 1   MASLQSLLKPVSHPLISPTEFFYDFQRPLRFARLLSLSVHESRNSRQLSSRGFRIRSSIA 60

Query: 61  DTVDRISDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHM 120
           DT+   SDAPVL SS+VLERLRASREN E+Q QFLAMYSSV GGITTDPA MVIPIDDHM
Sbjct: 61  DTIGHSSDAPVLSSSEVLERLRASRENLESQQQFLAMYSSVFGGITTDPAAMVIPIDDHM 120

Query: 121 VHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK 180
           VHRGHGVFDTAAIVDG+LYELDQHLDRILRSASMAKINLPV YDRE IK ILIRT SASK
Sbjct: 121 VHRGHGVFDTAAIVDGYLYELDQHLDRILRSASMAKINLPVPYDRETIKRILIRTVSASK 180

Query: 181 CRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFA 240
           CRNG LRYWLSAGPG+F LS+SG ++P+LYA+VIQGKPPS PKGIKVITSS+PMKPPQFA
Sbjct: 181 CRNGSLRYWLSAGPGDFQLSSSGCYEPSLYAVVIQGKPPSHPKGIKVITSSVPMKPPQFA 240

Query: 241 TMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKIL 300
            MKSVNYLPNVLS MEAEEKGAY +IWLD++GFIAEGP+MNV FIT DKEFLMPHFDKIL
Sbjct: 241 IMKSVNYLPNVLSKMEAEEKGAYASIWLDNDGFIAEGPNMNVAFITSDKEFLMPHFDKIL 300

Query: 301 SGCTAKRIITLAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQII 360
           SGCTAKRI+TLAE+LV EGRLRSIR ENLTMEEGKKA+EM+LIGSGVLV PVLQWDEQII
Sbjct: 301 SGCTAKRILTLAEQLVKEGRLRSIRCENLTMEEGKKADEMMLIGSGVLVCPVLQWDEQII 360

Query: 361 GDGREGPLARAIVDLIIEDMKFGPPTVRIPIPY 384
           GDGREGPLA+A++DL+IEDMK GP TVRIP+PY
Sbjct: 361 GDGREGPLAQALLDLLIEDMKSGPSTVRIPVPY 393

[Close]

BLAST of Carg00375 vs. ExPASy TrEMBL
Match: A0A5D3E5X5 (D-amino-acid transaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004930 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.9e-168
Identity = 304/393 (77.35%), Postives = 337/393 (85.75%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKS----------CNSRQLSFRRFRIRCSIA 60
           M SLQ +L+PIPQ+  S A L   SQ  +  +           N+R+L FR FRI  SIA
Sbjct: 1   MASLQSILRPIPQATNSPAVLPHRSQSSIRFTRFRSHHVQILRNTRRLYFRGFRIMASIA 60

Query: 61  DTVDRISDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHM 120
           DTVD  SDAPVL SS+V+ERLRA RENQENQ ++LAMYSSV GGITTDPA MVIPIDDHM
Sbjct: 61  DTVDHTSDAPVLTSSEVIERLRARRENQENQQEYLAMYSSVFGGITTDPAAMVIPIDDHM 120

Query: 121 VHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK 180
           VHRGHGVFDTAAIVDG+LYELDQHLDRIL+SASMAKINLP+ YDRE I+ ILIRT SASK
Sbjct: 121 VHRGHGVFDTAAIVDGYLYELDQHLDRILKSASMAKINLPIPYDREMIRRILIRTVSASK 180

Query: 181 CRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFA 240
           CRNG LRYWLSAGPG+F LS+SG H  ALYA+VIQGKPPS PKGIKVITSS+PMKPPQFA
Sbjct: 181 CRNGSLRYWLSAGPGDFQLSSSGCHLSALYAVVIQGKPPSRPKGIKVITSSVPMKPPQFA 240

Query: 241 TMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKIL 300
            MKSVNYLPNVLS MEAEEKGAY +IWLDS+GFIAEGP+MNV FIT DKEF+MPHFDKIL
Sbjct: 241 IMKSVNYLPNVLSKMEAEEKGAYASIWLDSDGFIAEGPNMNVAFITSDKEFIMPHFDKIL 300

Query: 301 SGCTAKRIITLAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQII 360
           SGCTAKRII LAERLV EG+LRSI  EN+TMEEGKKA+EM+LIGSGVLVSPVLQWDEQII
Sbjct: 301 SGCTAKRIINLAERLVKEGKLRSISCENITMEEGKKADEMMLIGSGVLVSPVLQWDEQII 360

Query: 361 GDGREGPLARAIVDLIIEDMKFGPPTVRIPIPY 384
           GDG+EGPL +A++DL+IEDMK GPPTVRIP+PY
Sbjct: 361 GDGKEGPLVQALLDLLIEDMKSGPPTVRIPVPY 393

[Close]

BLAST of Carg00375 vs. ExPASy TrEMBL
Match: A0A1S3CQM1 (D-amino-acid transaminase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503592 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.9e-168
Identity = 304/393 (77.35%), Postives = 337/393 (85.75%), Query Frame = 0


Query: 1   MNSLQFLLKPIPQSPISGAELTAHSQRPLDKS----------CNSRQLSFRRFRIRCSIA 60
           M SLQ +L+PIPQ+  S A L   SQ  +  +           N+R+L FR FRI  SIA
Sbjct: 1   MASLQSILRPIPQATNSPAVLPHRSQSSIRFTRFRSHHVQILRNTRRLYFRGFRIMASIA 60

Query: 61  DTVDRISDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGITTDPAVMVIPIDDHM 120
           DTVD  SDAPVL SS+V+ERLRA RENQENQ ++LAMYSSV GGITTDPA MVIPIDDHM
Sbjct: 61  DTVDHTSDAPVLTSSEVIERLRARRENQENQQEYLAMYSSVFGGITTDPAAMVIPIDDHM 120

Query: 121 VHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDREKIKNILIRTASASK 180
           VHRGHGVFDTAAIVDG+LYELDQHLDRIL+SASMAKINLP+ YDRE I+ ILIRT SASK
Sbjct: 121 VHRGHGVFDTAAIVDGYLYELDQHLDRILKSASMAKINLPIPYDREMIRRILIRTVSASK 180

Query: 181 CRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGIKVITSSIPMKPPQFA 240
           CRNG LRYWLSAGPG+F LS+SG H  ALYA+VIQGKPPS PKGIKVITSS+PMKPPQFA
Sbjct: 181 CRNGSLRYWLSAGPGDFQLSSSGCHLSALYAVVIQGKPPSRPKGIKVITSSVPMKPPQFA 240

Query: 241 TMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFITKDKEFLMPHFDKIL 300
            MKSVNYLPNVLS MEAEEKGAY +IWLDS+GFIAEGP+MNV FIT DKEF+MPHFDKIL
Sbjct: 241 IMKSVNYLPNVLSKMEAEEKGAYASIWLDSDGFIAEGPNMNVAFITSDKEFIMPHFDKIL 300

Query: 301 SGCTAKRIITLAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIGSGVLVSPVLQWDEQII 360
           SGCTAKRII LAERLV EG+LRSI  EN+TMEEGKKA+EM+LIGSGVLVSPVLQWDEQII
Sbjct: 301 SGCTAKRIINLAERLVKEGKLRSISCENITMEEGKKADEMMLIGSGVLVSPVLQWDEQII 360

Query: 361 GDGREGPLARAIVDLIIEDMKFGPPTVRIPIPY 384
           GDG+EGPL +A++DL+IEDMK GPPTVRIP+PY
Sbjct: 361 GDGKEGPLVQALLDLLIEDMKSGPPTVRIPVPY 393

[Close]

BLAST of Carg00375 vs. TAIR 10
Match: AT5G57850.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 439.1 bits (1128), Expect = 3.6e-123
Identity = 220/349 (63.04%), Postives = 281/349 (80.52%), Query Frame = 0


Query: 36  RQLSFRRFRIRCSIADTVDRISDAPVLGSSQVLERLRASRENQENQPQFLAMYSSVVGGI 95
           R+L+ RR  + CS  D+  +  + PVL S +V ERL+ +R  Q    QFLAMYSSVV GI
Sbjct: 33  RRLTRRRTILMCS--DSSSQSWNVPVLSSYEVGERLKLARGGQ----QFLAMYSSVVDGI 92

Query: 96  TTDPAVMVIPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSASMAKINLPVSYDR 155
           TTDPA MV+P+DDHMVHRGHGVFDTA I++G+LYELDQHLDRILRSASMAKI LP  +DR
Sbjct: 93  TTDPAAMVLPLDDHMVHRGHGVFDTALIINGYLYELDQHLDRILRSASMAKIPLP--FDR 152

Query: 156 EKIKNILIRTASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPALYAIVIQGKPPSPPKGI 215
           E IK ILI+T S S CR+G LRYWLSAGPG+FLLS S   +P LYAIVI+      P G+
Sbjct: 153 ETIKRILIQTVSVSGCRDGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTNFAINPIGV 212

Query: 216 KVITSSIPMKPPQFATMKSVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMNVGFI 275
           KV+TSSIP+KPP+FAT+KSVNYLPNVLS MEAE KGAY  IW+  +GFIAEGP+MNV F+
Sbjct: 213 KVVTSSIPIKPPEFATVKSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEGPNMNVAFV 272

Query: 276 TK-DKEFLMPHFDKILSGCTAKRIITLAERLVTEGRLRSIRFENLTMEEGKKAEEMVLIG 335
               KE +MP FD +LSGCTAKR +TLAE+LV++G L++++  ++T+E+GKKA+EM+LIG
Sbjct: 273 VNGGKELVMPRFDNVLSGCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKKADEMMLIG 332

Query: 336 SGVLVSPVLQWDEQIIGDGREGPLARAIVDLIIEDMKFGPPTVRIPIPY 384
           SG+ + PV+QWDE+ IG+G+EGP+A+A++DL++EDM+ GPP+VR+ +PY
Sbjct: 333 SGIPIRPVIQWDEEFIGEGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]

BLAST of Carg00375 vs. TAIR 10
Match: AT3G05190.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 6.4e-19
Identity = 84/294 (28.57%), Postives = 142/294 (48.30%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE++K  +
Sbjct: 278 VSVFDSVVQGGDSVWEGLRIYKGKIFKLEEHLDRLFDSAKALAFDNVPA---REEVKEAI 337

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPA------LYAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 338 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAYNRYGCTLIVLAEWKPPVYDNEG 397

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+    A  AI LD +G+++E  + N
Sbjct: 398 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNTNAADAIMLDKDGYVSETNATN 457

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLA--ERLVTEGRLRSIRFENLTMEEGKKAEE 343
           + F+ K    L PH D  L G T   ++ L   E  + E R        +++ E   A E
Sbjct: 458 I-FMVKKGCVLTPHADYCLPGITRATVMELVVKENFILEER-------RISLSEFHTANE 517

Query: 344 MVLIGSGVLVSPVLQWDEQIIGDGREGPLARAIVDL---IIEDMKFGPPTVRIP 381
           +   G+   +SPV++ D ++IGDG+ GP+ R + +    + ED     PT + P
Sbjct: 518 VWTTGTMGELSPVVKIDGRVIGDGKVGPVTRTLQNAYKKLTEDSGVPIPTYQEP 555

[Close]

BLAST of Carg00375 vs. TAIR 10
Match: AT5G27410.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-18
Identity = 79/273 (28.94%), Postives = 135/273 (49.45%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE+IK  +
Sbjct: 275 VSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT---REEIKEAI 334

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPAL------YAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 335 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAFNRYGCTLIVLAEWKPPVYDNDG 394

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+       AI LD +GF++E  + N
Sbjct: 395 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETNATN 454

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLA--ERLVTEGRLRSIRFENLTMEEGKKAEE 343
           +  + KD+  L PH D  L G T   ++ L   E  + E R        +++ E   A+E
Sbjct: 455 IFMVKKDR-VLTPHADYCLPGITRATVMELVVKENFILEER-------RISLSEFHTADE 514

Query: 344 MVLIGSGVLVSPVLQWDEQIIGDGREGPLARAI 363
           +   G+   +SPV++ D ++IG+G+ GP+ R +
Sbjct: 515 VWTTGTMGELSPVVKIDGRVIGEGKVGPVTRRL 531

[Close]

BLAST of Carg00375 vs. TAIR 10
Match: AT5G27410.2 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-18
Identity = 79/273 (28.94%), Postives = 135/273 (49.45%), Query Frame = 0


Query: 104 IPIDDHMVHRGHGVFDTAAIVDGHLYELDQHLDRILRSA-SMAKINLPVSYDREKIKNIL 163
           + + D +V  G  V++   I  G +++L++HLDR+  SA ++A  N+P    RE+IK  +
Sbjct: 652 VSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT---REEIKEAI 711

Query: 164 IRT-ASASKCRNGQLRYWLSAGPGNFLLSTSGLHQPAL------YAIVIQGKPP--SPPK 223
            RT  +     N  +R  L+ G       TSG+  PA         ++ + KPP      
Sbjct: 712 FRTLITNGMFDNTHIRLSLTRGK----KVTSGM-SPAFNRYGCTLIVLAEWKPPVYDNDG 771

Query: 224 GIKVITSSIPMKPPQFATMK--SVNYLPNVLSNMEAEEKGAYTAIWLDSEGFIAEGPSMN 283
           GI ++T++     P     K    N L N+L+ +E+       AI LD +GF++E  + N
Sbjct: 772 GIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETNATN 831

Query: 284 VGFITKDKEFLMPHFDKILSGCTAKRIITLA--ERLVTEGRLRSIRFENLTMEEGKKAEE 343
           +  + KD+  L PH D  L G T   ++ L   E  + E R        +++ E   A+E
Sbjct: 832 IFMVKKDR-VLTPHADYCLPGITRATVMELVVKENFILEER-------RISLSEFHTADE 891

Query: 344 MVLIGSGVLVSPVLQWDEQIIGDGREGPLARAI 363
           +   G+   +SPV++ D ++IG+G+ GP+ R +
Sbjct: 892 VWTTGTMGELSPVVKIDGRVIGEGKVGPVTRRL 908

The following BLAST results are available for this feature:

BLAST of Carg00375 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7031718.1	2.0e-216	100.00	D-amino-acid transaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022940281.1	8.4e-215	99.22	D-amino-acid transaminase, chloroplastic [Cucurbita moschata]	[more]
XP_023521525.1	5.3e-209	97.14	D-amino-acid transaminase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022981012.1	1.2e-208	97.14	D-amino-acid transaminase, chloroplastic [Cucurbita maxima]	[more]
KAG6608083.1	6.9e-193	92.19	D-amino-acid transaminase, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of Carg00375 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L493	5.1e-122	63.04	D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT...	[more]
Q58414	4.6e-22	26.82	Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannas...	[more]
O29329	1.3e-21	27.74	Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (s...	[more]
Q8W0Z7	9.0e-18	28.57	Branched-chain-amino-acid aminotransferase-like protein 1 OS=Arabidopsis thalian...	[more]
Q9ASR4	1.5e-17	28.94	Branched-chain-amino-acid aminotransferase-like protein 2 OS=Arabidopsis thalian...	[more]

BLAST of Carg00375 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FI15	4.1e-215	99.22	D-amino-acid transaminase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1IST6	5.7e-209	97.14	D-amino-acid transaminase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A6J1CNI4	4.7e-171	79.64	D-amino-acid transaminase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A5D3E5X5	4.9e-168	77.35	D-amino-acid transaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CQM1	4.9e-168	77.35	D-amino-acid transaminase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503592...	[more]

BLAST of Carg00375 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G57850.1	3.6e-123	63.04	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT3G05190.1	6.4e-19	28.57	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT5G27410.1	1.1e-18	28.94	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT5G27410.2	1.1e-18	28.94	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043131	Branched-chain-amino-acid aminotransferase-like, N-terminal	GENE3D	3.30.470.10		coord: 56..204 e-value: 1.4E-23 score: 85.3
IPR043132	Branched-chain-amino-acid aminotransferase-like, C-terminal	GENE3D	3.20.10.10		coord: 210..372 e-value: 1.2E-37 score: 130.9
IPR001544	Aminotransferase class IV	PFAM	PF01063	Aminotran_4	coord: 116..344 e-value: 2.8E-42 score: 145.1
None	No IPR available	PANTHER	PTHR42743:SF8	BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE-LIKE	coord: 55..382
None	No IPR available	PANTHER	PTHR42743	AMINO-ACID AMINOTRANSFERASE	coord: 55..382
IPR036038	Aminotransferase-like, PLP-dependent enzymes	SUPERFAMILY	56752	D-aminoacid aminotransferase-like PLP-dependent enzymes	coord: 93..369

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00375-RA	Carg00375-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019752	carboxylic acid metabolic process
molecular_function	GO:0008483	transaminase activity
molecular_function	GO:0003824	catalytic activity