Carg00333 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00333
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRNase H domain-containing protein
LocationCarg_Chr04: 6650385 .. 6651781 (+)
RNA-Seq ExpressionCarg00333
SyntenyCarg00333
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGTACATGGCCTACTACTTCATGTTTTCACACCGCCAGCGTGCTATTGGCGGATCGGGAGATGCACTCCGGCATACTACATACCGACACTTACAAATCCTGGTAGGCATGTATTTAAGAATCCCTCCTTTCATGATGTACATATATAAGCCAATGAGATCACCTGGTGTATGTAAATTATTAGGCTAGCAGAAGGAAACCATGGAAAAGGATTAGAAACACTGAGAAACTAGAAAAAAAAAATGAAGAGAAGCTAGAAGTTAGAGGTGAGATTTTTGGAATTGGAAGTTGAATTATAATTTAGAAGAACAGAGGGATCTTTGAAGGGCTAAAGAGAACCTGGATTATAGAGGTCTGTGATATTGATGATGATGATGGTCATTGATGTGGGCTTGGAGAGCTGTGAAGATGGTGCAGATTTTGAAAATTTTGGCTTTGCTTTGCTTAGAGAGCTAGCATTAGCACCCTCATTTAGATGAATAGGAACAAGAATGTTGTATAGAAATTCATTTATTGGTGCATTTGTTGCTATTTGCATTATCTCCTACAGTCTCTAGATGGAAGAACTAACCCAAATTCTGATCAGAGCTTCATTTTTTCCTTTCGGCGAAAGAGTAGAGATTTGAACATTTAACCAAAATTTTAAATTTTTAATCGACTTCCTCCTTATAATTGAACTCGTTCTTACCAAAAGCTAAATTAAAATAGAACTGAGTCTTAATTTCCTATTTTTGGTATTGGAAATTATGTTTCTTACATGGCTTTTGAGTTTTTTTTGTCCTTCAAGGCCAAACTTCATGTGAAGCAATTATCCTGAAATTTCACATATGCTGCAAACTGGGATTCTTAGGGAAAGCTAAGCCTTCAGGATTTAAATTTGTTGAAAAGTCAATGAAACTGTGAACGATTTCTTCAAGGGAACAATGAAACTAAATTCTCAACGTCCTTCATGAACAAGAGAATTTTGGTCTAAAGTATAATTTTATTGCTAAACAATAAAGTGAAGGCAGAAATGTTCCTGGGTTTTGAAAGAAGGCTGAAGAAATCAGCCAAGGGCAGGGCAGGGCAGGTTACTAAGGTGATACAAACGTGTAAGCACAATCTCAAGAATGGGATTGGGATTCAGCAACAGAGGGTTCGGGTGCAGAGTTTTGGGCTTCTTCTAATAGCTTCATTTGACGCTCAGCATGTGTTCTCTCTCACAGGCACTAATTCCCATTATGATATCAAGCATTGTACCAGTTGTGCCAAAAAACACAAGGGGTGCCAGAGACTTCCTCTTTATTCCCACTGGAATTGCTAGCAACGCTCCTGCTACTGAGAATACCCAAGTTCCCAACGCACTGTGTGAATGTTCAATGACAAGTAAGAATCAACCCAAAATCAACGCTTAA

mRNA sequence

ATGCAGTACATGGCCTACTACTTCATGTTTTCACACCGCCAGCGTGCTATTGGCGGATCGGGAGATGCACTCCGGCATACTACATACCGACACTTACAAATCCTGGCAGAAATGTTCCTGGGTTTTGAAAGAAGGCTGAAGAAATCAGCCAAGGGCAGGGCAGGGCAGGTTACTAAGGTGATACAAACGTGTAAGCACAATCTCAAGAATGGGATTGGGATTCAGCAACAGAGGGTTCGGGTGCAGAGTTTTGGGCTTCTTCTAATAGCTTCATTTGACGCTCAGCATGCACTAATTCCCATTATGATATCAAGCATTGTACCAGTTGTGCCAAAAAACACAAGGGGTGCCAGAGACTTCCTCTTTATTCCCACTGGAATTGCTAGCAACGCTCCTGCTACTGAGAATACCCAAGTTCCCAACGCACTGTGTGAATGTTCAATGACAAGTAAGAATCAACCCAAAATCAACGCTTAA

Coding sequence (CDS)

ATGCAGTACATGGCCTACTACTTCATGTTTTCACACCGCCAGCGTGCTATTGGCGGATCGGGAGATGCACTCCGGCATACTACATACCGACACTTACAAATCCTGGCAGAAATGTTCCTGGGTTTTGAAAGAAGGCTGAAGAAATCAGCCAAGGGCAGGGCAGGGCAGGTTACTAAGGTGATACAAACGTGTAAGCACAATCTCAAGAATGGGATTGGGATTCAGCAACAGAGGGTTCGGGTGCAGAGTTTTGGGCTTCTTCTAATAGCTTCATTTGACGCTCAGCATGCACTAATTCCCATTATGATATCAAGCATTGTACCAGTTGTGCCAAAAAACACAAGGGGTGCCAGAGACTTCCTCTTTATTCCCACTGGAATTGCTAGCAACGCTCCTGCTACTGAGAATACCCAAGTTCCCAACGCACTGTGTGAATGTTCAATGACAAGTAAGAATCAACCCAAAATCAACGCTTAA

Protein sequence

MQYMAYYFMFSHRQRAIGGSGDALRHTTYRHLQILAEMFLGFERRLKKSAKGRAGQVTKVIQTCKHNLKNGIGIQQQRVRVQSFGLLLIASFDAQHALIPIMISSIVPVVPKNTRGARDFLFIPTGIASNAPATENTQVPNALCECSMTSKNQPKINA
Homology
BLAST of Carg00333 vs. NCBI nr
Match: KAG7031676.1 (hypothetical protein SDJN02_05717, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313.2 bits (801), Expect = 1.3e-81
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0

Query: 1   MQYMAYYFMFSHRQRAIGGSGDALRHTTYRHLQILAEMFLGFERRLKKSAKGRAGQVTKV 60
           MQYMAYYFMFSHRQRAIGGSGDALRHTTYRHLQILAEMFLGFERRLKKSAKGRAGQVTKV
Sbjct: 1   MQYMAYYFMFSHRQRAIGGSGDALRHTTYRHLQILAEMFLGFERRLKKSAKGRAGQVTKV 60

Query: 61  IQTCKHNLKNGIGIQQQRVRVQSFGLLLIASFDAQHALIPIMISSIVPVVPKNTRGARDF 120
           IQTCKHNLKNGIGIQQQRVRVQSFGLLLIASFDAQHALIPIMISSIVPVVPKNTRGARDF
Sbjct: 61  IQTCKHNLKNGIGIQQQRVRVQSFGLLLIASFDAQHALIPIMISSIVPVVPKNTRGARDF 120

Query: 121 LFIPTGIASNAPATENTQVPNALCECSMTSKNQPKINA 159
           LFIPTGIASNAPATENTQVPNALCECSMTSKNQPKINA
Sbjct: 121 LFIPTGIASNAPATENTQVPNALCECSMTSKNQPKINA 158

BLAST of Carg00333 vs. NCBI nr
Match: OMO62607.1 (hypothetical protein CCACVL1_22724 [Corchorus capsularis])

HSP 1 Score: 80.9 bits (198), Expect = 1.1e-11
Identity = 41/47 (87.23%), Postives = 41/47 (87.23%), Query Frame = 0

Query: 97  ALIPIMISSIVPVVPKNTRGARDFLFIPTGIASNAPATENTQVPNAL 144
           ALIPIMISSIVPVVPKNTRGA DF  IPTGIAS APATE T VPNAL
Sbjct: 364 ALIPIMISSIVPVVPKNTRGANDFRLIPTGIASKAPATEKTHVPNAL 410

BLAST of Carg00333 vs. NCBI nr
Match: KAG6608047.1 (Protein ROLLING AND ERECT LEAF 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 76.3 bits (186), Expect = 2.7e-10
Identity = 35/39 (89.74%), Postives = 36/39 (92.31%), Query Frame = 0

Query: 2   QYMAYYFMFSHRQRAIGGSGDALRHTTYRHLQILAEMFL 41
           QYMAYYFMFSHRQRAIGGSGDALRHTTYRHLQIL  + L
Sbjct: 911 QYMAYYFMFSHRQRAIGGSGDALRHTTYRHLQILRSVIL 949

BLAST of Carg00333 vs. NCBI nr
Match: KAE8077422.1 (hypothetical protein FH972_015987 [Carpinus fangiana])

HSP 1 Score: 75.5 bits (184), Expect = 4.6e-10
Identity = 37/47 (78.72%), Postives = 41/47 (87.23%), Query Frame = 0

Query: 97  ALIPIMISSIVPVVPKNTRGARDFLFIPTGIASNAPATENTQVPNAL 144
           A+IP++ISS+VPVVPKNTRGARDF FIPTGIAS APATE T VP  L
Sbjct: 411 AVIPMIISSMVPVVPKNTRGARDFRFIPTGIASKAPATEKTHVPMGL 457

BLAST of Carg00333 vs. NCBI nr
Match: CAE5964481.1 (unnamed protein product [Arabidopsis arenosa])

HSP 1 Score: 68.6 bits (166), Expect = 5.7e-08
Identity = 34/46 (73.91%), Postives = 38/46 (82.61%), Query Frame = 0

Query: 98   LIPIMISSIVPVVPKNTRGARDFLFIPTGIASNAPATENTQVPNAL 144
            L  IM+SSIVPVVPK T GARDF F+PTGIA+ AP TENT VP+AL
Sbjct: 1058 LYMIMMSSIVPVVPKKTSGARDFRFMPTGIATKAPKTENTHVPSAL 1103

BLAST of Carg00333 vs. ExPASy TrEMBL
Match: A0A1R3GX32 (Uncharacterized protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_22724 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 5.4e-12
Identity = 41/47 (87.23%), Postives = 41/47 (87.23%), Query Frame = 0

Query: 97  ALIPIMISSIVPVVPKNTRGARDFLFIPTGIASNAPATENTQVPNAL 144
           ALIPIMISSIVPVVPKNTRGA DF  IPTGIAS APATE T VPNAL
Sbjct: 364 ALIPIMISSIVPVVPKNTRGANDFRLIPTGIASKAPATEKTHVPNAL 410

BLAST of Carg00333 vs. ExPASy TrEMBL
Match: A0A5N6RES6 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_015987 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.2e-10
Identity = 37/47 (78.72%), Postives = 41/47 (87.23%), Query Frame = 0

Query: 97  ALIPIMISSIVPVVPKNTRGARDFLFIPTGIASNAPATENTQVPNAL 144
           A+IP++ISS+VPVVPKNTRGARDF FIPTGIAS APATE T VP  L
Sbjct: 411 AVIPMIISSMVPVVPKNTRGARDFRFIPTGIASKAPATEKTHVPMGL 457

BLAST of Carg00333 vs. ExPASy TrEMBL
Match: A0A0A9D4B1 (Uncharacterized protein OS=Arundo donax OX=35708 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 4.2e-09
Identity = 33/48 (68.75%), Postives = 40/48 (83.33%), Query Frame = 0

Query: 96  HALIPIMISSIVPVVPKNTRGARDFLFIPTGIASNAPATENTQVPNAL 144
           H L+P+M+SS+VPVVPK T GA+DF F+PTGIA+ APATE  QVP AL
Sbjct: 52  HWLMPMMMSSMVPVVPKKTSGAKDFFFMPTGIATRAPATEKNQVPRAL 99

BLAST of Carg00333 vs. ExPASy TrEMBL
Match: A0A7C9D2D2 (Uncharacterized protein (Fragment) OS=Opuntia streptacantha OX=393608 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.1e-08
Identity = 34/47 (72.34%), Postives = 38/47 (80.85%), Query Frame = 0

Query: 97  ALIPIMISSIVPVVPKNTRGARDFLFIPTGIASNAPATENTQVPNAL 144
           ALIPIM+SS+VPVVPK T GA DFL +PTGIA+ AP TE T VP AL
Sbjct: 7   ALIPIMMSSMVPVVPKKTSGASDFLLMPTGIATKAPNTEKTHVPKAL 53

BLAST of Carg00333 vs. ExPASy TrEMBL
Match: A0A7J6HLW3 (RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_013134 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.6e-08
Identity = 35/42 (83.33%), Postives = 37/42 (88.10%), Query Frame = 0

Query: 102 MISSIVPVVPKNTRGARDFLFIPTGIASNAPATENTQVPNAL 144
           MI SIV VVPKNT GA+DFLFIPT IA+NAPATENTQVP AL
Sbjct: 1   MIWSIVLVVPKNTIGAKDFLFIPTRIANNAPATENTQVPRAL 42

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031676.11.3e-81100.00hypothetical protein SDJN02_05717, partial [Cucurbita argyrosperma subsp. argyro... [more]
OMO62607.11.1e-1187.23hypothetical protein CCACVL1_22724 [Corchorus capsularis][more]
KAG6608047.12.7e-1089.74Protein ROLLING AND ERECT LEAF 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
KAE8077422.14.6e-1078.72hypothetical protein FH972_015987 [Carpinus fangiana][more]
CAE5964481.15.7e-0873.91unnamed protein product [Arabidopsis arenosa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1R3GX325.4e-1287.23Uncharacterized protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_22724 PE=4 ... [more]
A0A5N6RES62.2e-1078.72Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_015987 PE=4 SV=1[more]
A0A0A9D4B14.2e-0968.75Uncharacterized protein OS=Arundo donax OX=35708 PE=4 SV=1[more]
A0A7C9D2D22.1e-0872.34Uncharacterized protein (Fragment) OS=Opuntia streptacantha OX=393608 PE=4 SV=1[more]
A0A7J6HLW33.6e-0883.33RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_013134 PE=... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00333-RACarg00333-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity