Carg00330 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00330
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptiondnaJ homolog subfamily B member 5-like
LocationCarg_Chr04: 6628611 .. 6630681 (+)
RNA-Seq ExpressionCarg00330
SyntenyCarg00330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGATCTTCACCAAGTTCTTGGCGTCTCCTTTCGAAACTTCTGCAAATCCTACAAATCCTTCTTTAAAAAATGGCATTCTAAGGTCAAGAATTCCAAGAAGAATGCCGATGTTGTTGATTCGAAGCACGACGGCTCGTCCGACGCCATTGATAACAACAACAATGGACCTTCTAAGGTACGTCCATTCTTCGGTTACGTTTCTGGTTTAATCAGGCTTTGAATGTTTTAGTTTTTTATTTTTCTTAATGATTAAGCGTGTTTATACGATTTTGAATTCGAATTCGTTGTGATTTATTTTTCTATTTTGTTCCGATTCTGTATTGTTTGATTGATTGATCTGCGGATGATTTTGATCGAGCGTGTAAAACGACGAAGATGCCCTAATTTAAAGAAATTTTGGCGTTTAATCGATAATAATCATTGTTTTCAGGATCGTGAAGAAATTAATAAGGAAGGACCGCGACAGATGACGAAGAAAGGCGGTTTGAGCTTTAGATATGCAAGCTGGAGTATGAGGGAGAACGATCCGAAGATTTCCAGGCATCGAAGCTATGATAGTTCTATGTTCAGGAATGCGAGTCGGAGAGGTCTAAATCCGCAGCCGTCGTCATCGTCTCAGTTTCGGAGCGTAAGCAGGAGGAGCACGGAATCGATGCCGTCTCGGTTAAACAGCGGGCTGAGCGTCGATTCGGCGTCGTCTTCTCCAATGCTGTCTAAAAGCGGTAGCCGGAGGAGCACGACGCCGATCATGTTCTCGAATTCCTCCGGTGTGCTGAAACCGCCGCCGATCGAGAAGCAGCTTGAATGCACTCTGGAGGAGCTCTGCTTTGGATGCATCAAGAAAATTAAGGTCACAAGAGACGTCATCTTGAACACAGGGTTCGTCCTCTCTGCTTCTTAATTTCTTTAGATTATTGATACATAATATTTCCATTCTGAATATTTTAAAAAATATTGTTCAATATATTATACTTTTAATTAACAAAAAAATTCGAAAATGAAAAAAATCGATGAGGCTAATTTAAGGAATGATCATGGAACTTATAACCAAGAATACCAGGAATACCATCTTTATTAGTATGAGACTTATAACCAGGGCATGGACTTATAACCAGGAATACCATATTTATTAGTATGAGACCTTTTGGGGAAGCCCAAAGCAAAGTCATGAGAATTTATTTTCATATCCATGATTCCTAACATGGTATCAGAGTCATGTCCTTAAATTAGCAATGTCAAGCCTCGAAGGTATAGTCAAAAGTGACTAAAGTGTCGAACAAAGTGTTCGAGGGCTCCATAGGTCTCAGGGGAGGATTGTTGAGGATTGTTGGAATAGTATCTTCATTGGTATGAGGCCTTTTGAGGGAGTCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGTAATATCATACCATGGTGGAGATCCGTAATTCCTAACAAGTTATAAGAGCTTATGTTCAAAGTGGACAATATCATACTATTGTGGAGATCCGTGATTTCTAACAAATTATATATCCGATTACAATTATTCAATGGTTTCTACGAATTTAATTTGTCATGCTGTCATTGTCGTAGCTTCTAAAAGTAAAGATATTTCATTGTTGATTGACAGGCAAGCAATAGAGGAAGAGGAAACGGTAACGATAAGAGTGAAACCAGGGTGGACGAAGGGGACAAAGATAACATTTGAAGGAATGGGGAATGAAAGGCCGGGTAGTTATCCAGCTGACGTATCGTTCGTGATAGTTGAGAAACAACACTTATATTTCAAAAGAGAAGGGGACGATTTGAAGCTAACGGTGGAAATTCCTTTGTTGAAAGCCCTAACTGGTTGCACCATTTCAATACCTTTGTTGGGAGGAGATGTAATGAGCCTCAGGACACATGACGTCATTTGGCATGGTTGTGTGAAGATCATTAAAGGTCAAGGTATGCCAAAACTTAAAGAGGAAGCAAATAGAGGCAACTTAAAAGTTAAGTTTTTAATCGAGTTTCCAACTGAATTAACTGACCAACAAAGATCCGACGTATGTCGAATTTTGGAAGGTTCTAACTATTCTTAG

mRNA sequence

ATGGTGGATCTTCACCAAGTTCTTGGCGTCTCCTTTCGAAACTTCTGCAAATCCTACAAATCCTTCTTTAAAAAATGGCATTCTAAGGTCAAGAATTCCAAGAAGAATGCCGATGTTGTTGATTCGAAGCACGACGGCTCGTCCGACGCCATTGATAACAACAACAATGGACCTTCTAAGGATCGTGAAGAAATTAATAAGGAAGGACCGCGACAGATGACGAAGAAAGGCGGTTTGAGCTTTAGATATGCAAGCTGGAGTATGAGGGAGAACGATCCGAAGATTTCCAGGCATCGAAGCTATGATAGTTCTATGTTCAGGAATGCGAGTCGGAGAGGTCTAAATCCGCAGCCGTCGTCATCGTCTCAGTTTCGGAGCGTAAGCAGGAGGAGCACGGAATCGATGCCGTCTCGGTTAAACAGCGGGCTGAGCGTCGATTCGGCGTCGTCTTCTCCAATGCTGTCTAAAAGCGGTAGCCGGAGGAGCACGACGCCGATCATGTTCTCGAATTCCTCCGGTGTGCTGAAACCGCCGCCGATCGAGAAGCAGCTTGAATGCACTCTGGAGGAGCTCTGCTTTGGATGCATCAAGAAAATTAAGGTCACAAGAGACGTCATCTTGAACACAGGGCAAGCAATAGAGGAAGAGGAAACGGTAACGATAAGAGTGAAACCAGGGTGGACGAAGGGGACAAAGATAACATTTGAAGGAATGGGGAATGAAAGGCCGGGTAGTTATCCAGCTGACGTATCGTTCGTGATAGTTGAGAAACAACACTTATATTTCAAAAGAGAAGGGGACGATTTGAAGCTAACGGTGGAAATTCCTTTGTTGAAAGCCCTAACTGGTTGCACCATTTCAATACCTTTGTTGGGAGGAGATGTAATGAGCCTCAGGACACATGACGTCATTTGGCATGGTTGTGTGAAGATCATTAAAGGTCAAGGTATGCCAAAACTTAAAGAGGAAGCAAATAGAGGCAACTTAAAAGTTAAGTTTTTAATCGAGTTTCCAACTGAATTAACTGACCAACAAAGATCCGACGTATGTCGAATTTTGGAAGGTTCTAACTATTCTTAG

Coding sequence (CDS)

ATGGTGGATCTTCACCAAGTTCTTGGCGTCTCCTTTCGAAACTTCTGCAAATCCTACAAATCCTTCTTTAAAAAATGGCATTCTAAGGTCAAGAATTCCAAGAAGAATGCCGATGTTGTTGATTCGAAGCACGACGGCTCGTCCGACGCCATTGATAACAACAACAATGGACCTTCTAAGGATCGTGAAGAAATTAATAAGGAAGGACCGCGACAGATGACGAAGAAAGGCGGTTTGAGCTTTAGATATGCAAGCTGGAGTATGAGGGAGAACGATCCGAAGATTTCCAGGCATCGAAGCTATGATAGTTCTATGTTCAGGAATGCGAGTCGGAGAGGTCTAAATCCGCAGCCGTCGTCATCGTCTCAGTTTCGGAGCGTAAGCAGGAGGAGCACGGAATCGATGCCGTCTCGGTTAAACAGCGGGCTGAGCGTCGATTCGGCGTCGTCTTCTCCAATGCTGTCTAAAAGCGGTAGCCGGAGGAGCACGACGCCGATCATGTTCTCGAATTCCTCCGGTGTGCTGAAACCGCCGCCGATCGAGAAGCAGCTTGAATGCACTCTGGAGGAGCTCTGCTTTGGATGCATCAAGAAAATTAAGGTCACAAGAGACGTCATCTTGAACACAGGGCAAGCAATAGAGGAAGAGGAAACGGTAACGATAAGAGTGAAACCAGGGTGGACGAAGGGGACAAAGATAACATTTGAAGGAATGGGGAATGAAAGGCCGGGTAGTTATCCAGCTGACGTATCGTTCGTGATAGTTGAGAAACAACACTTATATTTCAAAAGAGAAGGGGACGATTTGAAGCTAACGGTGGAAATTCCTTTGTTGAAAGCCCTAACTGGTTGCACCATTTCAATACCTTTGTTGGGAGGAGATGTAATGAGCCTCAGGACACATGACGTCATTTGGCATGGTTGTGTGAAGATCATTAAAGGTCAAGGTATGCCAAAACTTAAAGAGGAAGCAAATAGAGGCAACTTAAAAGTTAAGTTTTTAATCGAGTTTCCAACTGAATTAACTGACCAACAAAGATCCGACGTATGTCGAATTTTGGAAGGTTCTAACTATTCTTAG

Protein sequence

MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSKDREEINKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSSSSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPIEKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS
Homology
BLAST of Carg00330 vs. NCBI nr
Match: KAG6608044.1 (DnaJ-like subfamily B member 13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031673.1 DnaJ-like subfamily B member 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 708.4 bits (1827), Expect = 3.2e-200
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK 60
           MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK
Sbjct: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK 60

Query: 61  DREEINKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120
           DREEINKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS
Sbjct: 61  DREEINKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120

Query: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180
           SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI
Sbjct: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180

Query: 181 EKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240
           EKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN
Sbjct: 181 EKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240

Query: 241 ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300
           ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT
Sbjct: 241 ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300

Query: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 360
           HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS
Sbjct: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 359

BLAST of Carg00330 vs. NCBI nr
Match: XP_022941087.1 (dnaJ homolog subfamily B member 5-like [Cucurbita moschata])

HSP 1 Score: 696.0 bits (1795), Expect = 1.6e-196
Identity = 354/359 (98.61%), Postives = 356/359 (99.16%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK 60
           MVDL QVLGVSFRNFCKSYKSFFKKWHSKVKNSKKN DVVDSKHDGSSDAIDNNNNGPSK
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTDVVDSKHDGSSDAIDNNNNGPSK 60

Query: 61  DREEINKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120
           DREEINKEGPRQMTKKGGLSFRYAS SMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS
Sbjct: 61  DREEINKEGPRQMTKKGGLSFRYASRSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120

Query: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180
           SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI
Sbjct: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180

Query: 181 EKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240
           EKQLECTLEELCFGC+KKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN
Sbjct: 181 EKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240

Query: 241 ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300
           ERPGSYPAD+SFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT
Sbjct: 241 ERPGSYPADISFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300

Query: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 360
           HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS
Sbjct: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 359

BLAST of Carg00330 vs. NCBI nr
Match: XP_023525805.1 (dnaJ homolog subfamily B member 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 685.3 bits (1767), Expect = 2.9e-193
Identity = 349/359 (97.21%), Postives = 351/359 (97.77%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK 60
           MVDL QVLGVSFRNFCKSYKSFFKKWHSKVKNSKKN DV DSKHDGSSDAIDNNNNGPSK
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTDVADSKHDGSSDAIDNNNNGPSK 60

Query: 61  DREEINKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120
           DREEINKEGPRQMTKKGGLSFR+ S SMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS
Sbjct: 61  DREEINKEGPRQMTKKGGLSFRHGSRSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120

Query: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180
           SSQFRSVSRRS ESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI
Sbjct: 121 SSQFRSVSRRSAESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180

Query: 181 EKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240
           EKQLECTLEELCFGC+KKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN
Sbjct: 181 EKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240

Query: 241 ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300
           ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT
Sbjct: 241 ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300

Query: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 360
           HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFL EFPTELTDQQRSDVCRILEGS YS
Sbjct: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLTEFPTELTDQQRSDVCRILEGSTYS 359

BLAST of Carg00330 vs. NCBI nr
Match: XP_022981017.1 (dnaJ homolog subfamily B member 1-like [Cucurbita maxima])

HSP 1 Score: 676.0 bits (1743), Expect = 1.8e-190
Identity = 344/359 (95.82%), Postives = 350/359 (97.49%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK 60
           MVDL QVLGVSFRNFCKSYKSFFKKWHSKVKNSKKN DVVDSKHDGSSDAIDNNNNGPSK
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTDVVDSKHDGSSDAIDNNNNGPSK 60

Query: 61  DREEINKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120
           DREEI+KEGPRQMTKKGGLSFRY S SMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS
Sbjct: 61  DREEISKEGPRQMTKKGGLSFRYGSRSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120

Query: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180
           SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKP PI
Sbjct: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPAPI 180

Query: 181 EKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240
           EKQLECTLEELCFGC+KKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN
Sbjct: 181 EKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240

Query: 241 ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300
           ER GSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPL+KALTGCTISIPLLGGD+MSL+T
Sbjct: 241 ERLGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLVKALTGCTISIPLLGGDIMSLKT 300

Query: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 360
            DVIWHGCVKIIKGQGMPKLKEE NRGNLKVKFL EFPTELTDQQRSD+CRILEGSNYS
Sbjct: 301 QDVIWHGCVKIIKGQGMPKLKEEINRGNLKVKFLTEFPTELTDQQRSDICRILEGSNYS 359

BLAST of Carg00330 vs. NCBI nr
Match: XP_038899191.1 (dnaJ homolog subfamily B member 4-like [Benincasa hispida])

HSP 1 Score: 523.1 bits (1346), Expect = 1.9e-144
Identity = 277/364 (76.10%), Postives = 311/364 (85.44%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK 60
           MVDL Q LG+SFRNFCKSYKSFFKKWHSK KNSKK+ADVVD+KHD +SDAI   NNGPSK
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKAKNSKKDADVVDTKHDATSDAI--GNNGPSK 60

Query: 61  DREEINK-EGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYD---SSMFRNASRRGLNP 120
           D EE+ K EGP   T K   S RY   SMREND    R RSYD   SS+ RNASRRG NP
Sbjct: 61  DHEEVKKTEGP--TTMKSVHSLRYGGQSMRENDATSFRPRSYDSGVSSLSRNASRRGQNP 120

Query: 121 QPSSSSQFRSVSRRSTESMPSRLNSG-LSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVL 180
             +SSS FRSVSRRSTES+PSR++SG  S+DS SSSP+LSKSGSR STTPIMFSNSSG+L
Sbjct: 121 SSTSSSLFRSVSRRSTESVPSRVSSGRRSIDSVSSSPLLSKSGSRMSTTPIMFSNSSGLL 180

Query: 181 KPPPIEKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITF 240
           KP PIEKQLECTLEELCFGC+KKIKVTRD+IL +G+ +EEEET+TI++KPGWTKGTKITF
Sbjct: 181 KPAPIEKQLECTLEELCFGCMKKIKVTRDLILISGKTMEEEETLTIKIKPGWTKGTKITF 240

Query: 241 EGMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDV 300
           EGMGNERPGSYPAD+SFVIVEK+H YFKREGDDL+LTVEIPLLKAL GC IS+PLLGG+ 
Sbjct: 241 EGMGNERPGSYPADMSFVIVEKRHPYFKREGDDLELTVEIPLLKALIGCNISVPLLGGET 300

Query: 301 MSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEG 360
           MSL+ HDV+W G  K+I+GQGMPKLK++  RGNLKVKFL+EFPTELTDQQR DVCRILE 
Sbjct: 301 MSLKIHDVVWPGYEKVIQGQGMPKLKDD-KRGNLKVKFLVEFPTELTDQQRYDVCRILED 359

BLAST of Carg00330 vs. ExPASy Swiss-Prot
Match: Q80Y75 (DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 7.1e-33
Identity = 70/170 (41.18%), Postives = 111/170 (65.29%), Query Frame = 0

Query: 178 PPIEKQLECTLEELCFGCIKKIKVTRDVI-LNTGQAIEEEETVTIRVKPGWTKGTKITFE 237
           PPIE+ L  +LE+L FGC KKIK++R V+  +   +  +++ +TI V+PGW +GT+ITFE
Sbjct: 138 PPIERDLYLSLEDLFFGCTKKIKISRRVLNEDRYSSTIKDKILTIDVRPGWRQGTRITFE 197

Query: 238 GMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVM 297
             G++ P   PAD+ F++ EK H  F+RE D+L     IPL KALT CT+ +  L   ++
Sbjct: 198 KEGDQGPNIIPADIIFIVKEKLHPRFRREHDNLFFVYPIPLGKALTCCTVEVKTLDDRLL 257

Query: 298 SLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQR 347
           ++  +D++     KI+ G+GMP  +  + +G+L + F I+FPT LT Q++
Sbjct: 258 NIPINDIVHPKYFKIVPGEGMPLPENPSKKGDLFIFFDIQFPTRLTPQKK 307

BLAST of Carg00330 vs. ExPASy Swiss-Prot
Match: P59910 (DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.1e-32
Identity = 70/170 (41.18%), Postives = 109/170 (64.12%), Query Frame = 0

Query: 178 PPIEKQLECTLEELCFGCIKKIKVTRDVILNTG-QAIEEEETVTIRVKPGWTKGTKITFE 237
           P +E+ L  +LE+L FGC KKIK++R V+   G  +  +++ +TI VKPGW +GT+ITFE
Sbjct: 138 PQVERDLYLSLEDLFFGCTKKIKISRRVLNEDGYSSTIKDKILTIDVKPGWRQGTRITFE 197

Query: 238 GMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVM 297
             G++ P   PAD+ F++ EK H  F+RE D+L     IPL KALT CT+ +  L   ++
Sbjct: 198 KEGDQGPNIIPADIIFIVKEKLHPRFRRENDNLFFVNPIPLGKALTCCTVEVRTLDDRLL 257

Query: 298 SLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQR 347
           ++  +D+I     K + G+GMP  ++   +G+L + F I+FPT LT Q++
Sbjct: 258 NIPINDIIHPKYFKKVPGEGMPLPEDPTKKGDLFIFFDIQFPTRLTPQKK 307

BLAST of Carg00330 vs. ExPASy Swiss-Prot
Match: Q24133 (DnaJ protein homolog 1 OS=Drosophila melanogaster OX=7227 GN=DnaJ-1 PE=1 SV=3)

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-31
Identity = 75/177 (42.37%), Postives = 105/177 (59.32%), Query Frame = 0

Query: 178 PPIEKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEG 237
           PPIE  L  +LEE+  GCIKK+K++R    + G   +EE+ + I VKPGW  GTKITF  
Sbjct: 157 PPIEHDLFVSLEEVDKGCIKKMKISRMATGSNG-PYKEEKVLRITVKPGWKAGTKITFPQ 216

Query: 238 MGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMS 297
            G+  P   PAD+ F+I +K H  FKREG DLK T +I L +AL G  +S+P L G  + 
Sbjct: 217 EGDSAPNKTPADIVFIIRDKPHSLFKREGIDLKYTAQISLKQALCGALVSVPTLQGSRIQ 276

Query: 298 LR-THDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRIL 354
           +   H++I     + I G G+P  KE + RG+L V F I+FP  L    ++ +  +L
Sbjct: 277 VNPNHEIIKPTTTRRINGLGLPVPKEPSRRGDLIVSFDIKFPDTLAPSLQNQLSELL 332

BLAST of Carg00330 vs. ExPASy Swiss-Prot
Match: Q5BIP8 (DnaJ homolog subfamily B member 5 OS=Bos taurus OX=9913 GN=DNAJB5 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.3e-31
Identity = 72/190 (37.89%), Postives = 114/190 (60.00%), Query Frame = 0

Query: 158 GSRRSTTPIMFSNSSGVLKPPPIEKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEE 217
           G RR+  P+        ++ PP+  +L  +LEE+  G  K++K+TR  +   G+ +  E+
Sbjct: 152 GPRRAPEPLYPRRK---VQDPPVVHELRVSLEEIYHGSTKRMKITRRRLNPDGRTVRTED 211

Query: 218 TVT-IRVKPGWTKGTKITFEGMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIP 277
            +  I +K GW +GTKITF   G+  P + PAD+ FV+ +K H +F+R+G ++  +  I 
Sbjct: 212 KILHIVIKRGWKEGTKITFPKEGDATPDNIPADIVFVLKDKPHAHFRRDGTNVLYSALIS 271

Query: 278 LLKALTGCTISIPLLGGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIE 337
           L +AL GCT++IP + G V+ L  +DVI  G VK ++G+G+P  K    RG+L V+F + 
Sbjct: 272 LKEALCGCTVNIPTIDGRVIPLPCNDVIKPGTVKRLRGEGLPFPKVPTQRGDLIVEFKVR 331

Query: 338 FPTELTDQQR 347
           FP  LT Q R
Sbjct: 332 FPDRLTPQTR 338

BLAST of Carg00330 vs. ExPASy Swiss-Prot
Match: O75953 (DnaJ homolog subfamily B member 5 OS=Homo sapiens OX=9606 GN=DNAJB5 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.3e-31
Identity = 72/190 (37.89%), Postives = 114/190 (60.00%), Query Frame = 0

Query: 158 GSRRSTTPIMFSNSSGVLKPPPIEKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEE 217
           G RR+  P+        ++ PP+  +L  +LEE+  G  K++K+TR  +   G+ +  E+
Sbjct: 152 GPRRAPEPLYPRRK---VQDPPVVHELRVSLEEIYHGSTKRMKITRRRLNPDGRTVRTED 211

Query: 218 TVT-IRVKPGWTKGTKITFEGMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIP 277
            +  I +K GW +GTKITF   G+  P + PAD+ FV+ +K H +F+R+G ++  +  I 
Sbjct: 212 KILHIVIKRGWKEGTKITFPKEGDATPDNIPADIVFVLKDKPHAHFRRDGTNVLYSALIS 271

Query: 278 LLKALTGCTISIPLLGGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIE 337
           L +AL GCT++IP + G V+ L  +DVI  G VK ++G+G+P  K    RG+L V+F + 
Sbjct: 272 LKEALCGCTVNIPTIDGRVIPLPCNDVIKPGTVKRLRGEGLPFPKVPTQRGDLIVEFKVR 331

Query: 338 FPTELTDQQR 347
           FP  LT Q R
Sbjct: 332 FPDRLTPQTR 338

BLAST of Carg00330 vs. ExPASy TrEMBL
Match: A0A6J1FLF5 (dnaJ homolog subfamily B member 5-like OS=Cucurbita moschata OX=3662 GN=LOC111446483 PE=4 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 8.0e-197
Identity = 354/359 (98.61%), Postives = 356/359 (99.16%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK 60
           MVDL QVLGVSFRNFCKSYKSFFKKWHSKVKNSKKN DVVDSKHDGSSDAIDNNNNGPSK
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTDVVDSKHDGSSDAIDNNNNGPSK 60

Query: 61  DREEINKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120
           DREEINKEGPRQMTKKGGLSFRYAS SMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS
Sbjct: 61  DREEINKEGPRQMTKKGGLSFRYASRSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120

Query: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180
           SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI
Sbjct: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180

Query: 181 EKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240
           EKQLECTLEELCFGC+KKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN
Sbjct: 181 EKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240

Query: 241 ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300
           ERPGSYPAD+SFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT
Sbjct: 241 ERPGSYPADISFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300

Query: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 360
           HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS
Sbjct: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 359

BLAST of Carg00330 vs. ExPASy TrEMBL
Match: A0A6J1ISU0 (dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480292 PE=4 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 8.6e-191
Identity = 344/359 (95.82%), Postives = 350/359 (97.49%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK 60
           MVDL QVLGVSFRNFCKSYKSFFKKWHSKVKNSKKN DVVDSKHDGSSDAIDNNNNGPSK
Sbjct: 1   MVDLPQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNTDVVDSKHDGSSDAIDNNNNGPSK 60

Query: 61  DREEINKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120
           DREEI+KEGPRQMTKKGGLSFRY S SMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS
Sbjct: 61  DREEISKEGPRQMTKKGGLSFRYGSRSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSS 120

Query: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPI 180
           SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKP PI
Sbjct: 121 SSQFRSVSRRSTESMPSRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPAPI 180

Query: 181 EKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240
           EKQLECTLEELCFGC+KKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN
Sbjct: 181 EKQLECTLEELCFGCMKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGN 240

Query: 241 ERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRT 300
           ER GSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPL+KALTGCTISIPLLGGD+MSL+T
Sbjct: 241 ERLGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLVKALTGCTISIPLLGGDIMSLKT 300

Query: 301 HDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 360
            DVIWHGCVKIIKGQGMPKLKEE NRGNLKVKFL EFPTELTDQQRSD+CRILEGSNYS
Sbjct: 301 QDVIWHGCVKIIKGQGMPKLKEEINRGNLKVKFLTEFPTELTDQQRSDICRILEGSNYS 359

BLAST of Carg00330 vs. ExPASy TrEMBL
Match: A0A1S3BAK8 (chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103487597 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.9e-134
Identity = 269/367 (73.30%), Postives = 302/367 (82.29%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNAD-VVDSKH-DGSSDAIDNNNNGP 60
           MVDL Q LG+SFRNFCKSYKSFFKKWHSKVKNSKK+ D VVD+KH D +SDAI  +N+ P
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVDTKHDDATSDAI--HNHSP 60

Query: 61  SKDREEI-NKEGPRQMTKKGGL-SFRYASWSMRENDPKISRHRSYD---SSMFRNASRRG 120
           SKD EEI  KEGPR  T   G+ SFRY   S+REND    R RSYD   S++ RNASRRG
Sbjct: 61  SKDHEEIKKKEGPRPTTTTRGVHSFRYGGRSLRENDTTSFRPRSYDSGYSTLSRNASRRG 120

Query: 121 LNPQPSSSSQFRSVSRRSTESMPSRLNSG-LSVDSASSSPMLSKSGSRRSTTPIMFSNSS 180
            NP  +SSS FRSVSRRS ESMPSR++SG  S+DS SSSP+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 QNPASTSSSLFRSVSRRSNESMPSRVSSGRRSIDSISSSPVLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKPPPIEKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 240
           GVLK  PIEKQLECTLEELCFGC+KKIKVTRD++L  GQA+EEEET+TI+VKPGW KGTK
Sbjct: 181 GVLKAAPIEKQLECTLEELCFGCMKKIKVTRDLLLINGQAMEEEETLTIKVKPGWRKGTK 240

Query: 241 ITFE-GMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLL 300
           ITFE G GNER GSYPAD SFVI EK+H YFKREGDDL+L VEIPLLKALTGCTI +PLL
Sbjct: 241 ITFEGGTGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTIPVPLL 300

Query: 301 GGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCR 359
           GG+ MSL  H+V+  G  K+I+GQGMPKLK+  NRGNL VKF + FPT+LT QQRSDVCR
Sbjct: 301 GGETMSLEIHEVVSPGYEKLIQGQGMPKLKDPNNRGNLIVKFFVHFPTQLTPQQRSDVCR 360

BLAST of Carg00330 vs. ExPASy TrEMBL
Match: A0A0A0KYJ4 (DnaJ_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361870 PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.9e-130
Identity = 263/368 (71.47%), Postives = 302/368 (82.07%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNAD--VVDSKHDG-SSDAIDNNNNG 60
           MVDL Q LG+SFRNFCKSYKSFFKKWHSKVKNSKK+ D  VVD+KHD  +SDAI  +N G
Sbjct: 1   MVDLPQALGISFRNFCKSYKSFFKKWHSKVKNSKKDTDNVVVDTKHDDPTSDAI--HNYG 60

Query: 61  PSKDREEI-NKEGPRQMTKKGGLSFRYASWSMRENDPKISRHRSYD---SSMFRNASRRG 120
           PSKD E+I  KEGPR    KG  SFRY   S+REND    R +SYD   S++ RNASRRG
Sbjct: 61  PSKDHEDIKKKEGPR--PTKGVHSFRYGGRSLRENDTTSFRPQSYDSGYSTLSRNASRRG 120

Query: 121 LNPQPSSSSQFRSVSRRSTESMPSRLNSG-LSVDSASSSPMLSKSGSRRSTTPIMFSNSS 180
            N   +SSS FRS+SRRS ESM SR++SG  S+DS SSSP+LSKSGS+RSTTPIMFSNSS
Sbjct: 121 QNAGSTSSSLFRSMSRRSNESMTSRVSSGRRSIDSISSSPLLSKSGSKRSTTPIMFSNSS 180

Query: 181 GVLKPPPIEKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 240
           GVLK   IEKQLECTLEELCFGCIKKIKVTRD++L  GQA+EEEET+T++VKPGW KGTK
Sbjct: 181 GVLKAAAIEKQLECTLEELCFGCIKKIKVTRDLLLINGQAMEEEETLTMKVKPGWRKGTK 240

Query: 241 ITFE-GMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLL 300
           ITFE GMGNER GSYPAD SFVI EK+H YFKREGDDL+L VEIPLLKALTGCTIS+PLL
Sbjct: 241 ITFEGGMGNERAGSYPADTSFVIAEKRHSYFKREGDDLELMVEIPLLKALTGCTISVPLL 300

Query: 301 GGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCR 360
           GG+ MSL  H+V+  G  K+I+GQGMPKLK+   RG+L +KF ++FPT+LT QQRSDVCR
Sbjct: 301 GGETMSLDIHEVVSPGYEKLIQGQGMPKLKDPDTRGDLILKFFVDFPTQLTPQQRSDVCR 360

BLAST of Carg00330 vs. ExPASy TrEMBL
Match: A0A6J1BSE9 (uncharacterized protein LOC111005118 OS=Momordica charantia OX=3673 GN=LOC111005118 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 3.5e-128
Identity = 262/363 (72.18%), Postives = 296/363 (81.54%), Query Frame = 0

Query: 1   MVDLHQVLGVSFRNFCKSYKSFFKKWHSKVKNSKKNADVVDSKHDGSSDAIDNNNNGPSK 60
           MVDL Q +G+SFRNFCKSYKSFFKKWHSK+K+SKK++D VD K +GSSDAID    GPSK
Sbjct: 1   MVDLPQAIGISFRNFCKSYKSFFKKWHSKIKSSKKDSD-VDQKREGSSDAID----GPSK 60

Query: 61  DREEINKEGPRQMT---KKGGLSFRYASWSMREN-DPKISRHRSYDS--SMFRNASRRGL 120
           DRE++ +EG    T    KG  SFRY   SMREN DP   R RSYDS  S+ RNASR+  
Sbjct: 61  DREDVKREGRATATATATKGVPSFRYGG-SMRENHDPLSFRPRSYDSGFSLSRNASRKSQ 120

Query: 121 NPQPSSSSQFRSVSRRSTE-SMPSRLNSG-LSVDSASSSPMLSKSGSRRSTTPIMFSNSS 180
           N   SSS  FRSVSRRSTE S+PSR++SG  SVD  SS P+LSKSGSRRSTTPIMFSNSS
Sbjct: 121 NTH-SSSPLFRSVSRRSTESSVPSRVSSGRRSVDLVSSPPLLSKSGSRRSTTPIMFSNSS 180

Query: 181 GVLKPPPIEKQLECTLEELCFGCIKKIKVTRDVILN-TGQAIEEEETVTIRVKPGWTKGT 240
           GVLKP PIEKQLECTLEELCFGC KKIK+TRDV+ N TGQ  EEEET+TI VKPGW KGT
Sbjct: 181 GVLKPAPIEKQLECTLEELCFGCTKKIKITRDVLSNTTGQTTEEEETLTINVKPGWKKGT 240

Query: 241 KITFEGMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLL 300
           KITFEGMGNERPGSYPAD+SFVI EK+H +FK EGDDL++TVEIPLLKALTGCTIS+PLL
Sbjct: 241 KITFEGMGNERPGSYPADMSFVIAEKRHAFFKWEGDDLEMTVEIPLLKALTGCTISVPLL 300

Query: 301 GGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCR 355
           GG+ MSL   DV+  G  +II+GQGMP  K++  RG+LKVKFL+EFPT+LTDQQRSDVCR
Sbjct: 301 GGEAMSLEIDDVVSPGYERIIQGQGMPNPKDDDKRGDLKVKFLVEFPTQLTDQQRSDVCR 356

BLAST of Carg00330 vs. TAIR 10
Match: AT1G11040.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 251.9 bits (642), Expect = 7.7e-67
Identity = 134/273 (49.08%), Postives = 189/273 (69.23%), Query Frame = 0

Query: 87  SMRENDPKISRHRSYDSSMFRNASRRGLNPQPSSSSQFRSVSRRSTESMPSRLNSGLSVD 146
           S   N P     +S  SS+ ++AS+R  + + S+SS   S S   ++S  +R       D
Sbjct: 177 SPTSNSPINGVKQSSPSSISKSASKRDKDERGSTSSA-TSTSLPYSKSKSTR-------D 236

Query: 147 SASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPIEKQLECTLEELCFGCIKKIKVTRDVI 206
           +A S   ++KS SRRSTTPI+FS S+   KPP +EK+LECTLEELC G +K IK+ RD+I
Sbjct: 237 TAGS---IAKSISRRSTTPIVFSQSTPPKKPPAVEKKLECTLEELCHGGVKNIKIKRDII 296

Query: 207 LNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGNERPGSYPADVSFVIVEKQHLYFKREG 266
            + G  +++EE + + ++PGW KGTKITFEG+GNE+PG  P D++FV+ EK+H  FKR G
Sbjct: 297 TDEGLIMQQEEMLRVNIQPGWKKGTKITFEGVGNEKPGYLPEDITFVVEEKRHPLFKRRG 356

Query: 267 DDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANR 326
           DDL++ VEIPLLKALTGC +S+PLL G+ MS+   DVI+HG  K IKGQGMP  KEE  R
Sbjct: 357 DDLEIAVEIPLLKALTGCKLSVPLLSGESMSITVGDVIFHGFEKAIKGQGMPNAKEEGKR 416

Query: 327 GNLKVKFLIEFPTELTDQQRSDVCRILEGSNYS 360
           G+L++ FL+ FP +L+++QRS    +L+  +++
Sbjct: 417 GDLRITFLVNFPEKLSEEQRSMAYEVLKDCSWA 438

BLAST of Carg00330 vs. TAIR 10
Match: AT1G44160.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 224.2 bits (570), Expect = 1.7e-58
Identity = 145/341 (42.52%), Postives = 200/341 (58.65%), Query Frame = 0

Query: 25  KWHSKVKNSKKNADVVDSKHD-GSSDAID-NNNNGPSKDREEINKEGPRQMTKKGGLSFR 84
           K H K K+  K    +D +H   + +A +     G  K+    NK     + K     F 
Sbjct: 38  KHHHKAKHDTKETKRLDEEHKRNTPNAFEFRETKGIGKN----NKVEGVSLCKVRSYRFD 97

Query: 85  YASWSMRENDPKISRHRSYDSSMFRNASRRGLNPQPSSSS---QFRSVSRRSTES---MP 144
             + +   +   +SR  S ++     A+    NP   S S   + +S S R TES   MP
Sbjct: 98  NTNTNFVGSKKSLSRSCSQNT-----ATATSTNPTLRSLSFIGRSKSSSNRMTESGGFMP 157

Query: 145 SRLNSGLSVDSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPIEKQLECTLEELCFGCI 204
           + + S  +V  + ++P+L  S S +            V KP P EK+L CTLEELC GC 
Sbjct: 158 TLMRSTTTVPRSFANPILYSSSSAK------------VAKPSPTEKKLRCTLEELCNGCT 217

Query: 205 KKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGNERPGSYPADVSFVIVE 264
           KKIK+ RDVI + G+  EEEE V I+VKPGW  GTK+TFEG GNE   S PAD++FVIVE
Sbjct: 218 KKIKIKRDVITSLGEKCEEEEMVEIKVKPGWKGGTKVTFEGKGNEAMRSVPADLTFVIVE 277

Query: 265 KQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRTHDVIWHGCVKIIKGQG 324
           K+H  FKREGDDL++ VE+ LL+ALTGC +S+ LL GD M LR  DVI  G V +++G+G
Sbjct: 278 KEHEVFKREGDDLEMAVEVSLLEALTGCELSVALLDGDNMRLRIEDVIHPGYVTVVQGKG 337

Query: 325 MPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRILEGSN 358
           MP LKE+  RG+L+V+F  +FP  LTD+QR+++  IL+ S+
Sbjct: 338 MPNLKEKGKRGDLRVRFRTKFPQHLTDEQRAEIHSILQDSS 357

BLAST of Carg00330 vs. TAIR 10
Match: AT3G08910.1 (DNAJ heat shock family protein )

HSP 1 Score: 173.7 bits (439), Expect = 2.7e-43
Identity = 88/211 (41.71%), Postives = 135/211 (63.98%), Query Frame = 0

Query: 146 DSASSSPMLSKSGSRRSTTPIMFSNSSGVLKPPPIEKQLECTLEELCFGCIKKIKVTRDV 205
           DS  + P    S   R    +  SN     K  PIE+QL C+LE+L  G  KK+K++RDV
Sbjct: 117 DSRGAGP----SNGFRFAEDVFSSNVVPPRKAAPIERQLPCSLEDLYKGVSKKMKISRDV 176

Query: 206 ILNTGQAIEEEETVTIRVKPGWTKGTKITFEGMGNERPGSYPADVSFVIVEKQHLYFKRE 265
           + ++G+    EE +TI +KPGW KGTKITF   GNE+ G  P+D+ F++ EK H  FKR+
Sbjct: 177 LDSSGRPTTVEEILTIEIKPGWKKGTKITFPEKGNEQRGIIPSDLVFIVDEKPHAVFKRD 236

Query: 266 GDDLKLTVEIPLLKALTGCTISIPLLGGDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEAN 325
           G+DL +T +IPL++ALTG T  +  L G  +++  ++VI     +++KG+GMP  K+ + 
Sbjct: 237 GNDLVMTQKIPLVEALTGYTAQVSTLDGRSVTVPINNVISPSYEEVVKGEGMPIPKDPSK 296

Query: 326 RGNLKVKFLIEFPTELTDQQRSDVCRILEGS 357
           +GNL++KF ++FP+ LT +Q+S + R+   S
Sbjct: 297 KGNLRIKFTVKFPSRLTTEQKSGIKRMFSSS 323

BLAST of Carg00330 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 167.9 bits (424), Expect = 1.5e-41
Identity = 80/181 (44.20%), Postives = 124/181 (68.51%), Query Frame = 0

Query: 173 GVLKPPPIEKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 232
           G  K  PIE +L C+LE+L  G  KK++++R++   +G+ ++ EE +TI VKPGW KGTK
Sbjct: 156 GARKAAPIENKLPCSLEDLYKGTTKKMRISREIADVSGKTMQVEEILTIDVKPGWKKGTK 215

Query: 233 ITFEGMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLG 292
           ITF   GNE+PG  PAD+ F+I EK H  F REG+DL +T +I L++ALTG T+++  L 
Sbjct: 216 ITFPEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLIVTQKISLVEALTGYTVNLTTLD 275

Query: 293 GDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRI 352
           G  +++   +V+     +++  +GMP  K++  RGNL++KF I+FPT LT +Q++ V ++
Sbjct: 276 GRRLTIPVTNVVHPEYEEVVPKEGMPLQKDQTKRGNLRIKFNIKFPTRLTSEQKTGVKKL 335

Query: 353 L 354
           L
Sbjct: 336 L 336

BLAST of Carg00330 vs. TAIR 10
Match: AT2G20550.1 (HSP40/DnaJ peptide-binding protein )

HSP 1 Score: 166.8 bits (421), Expect = 3.2e-41
Identity = 78/181 (43.09%), Postives = 123/181 (67.96%), Query Frame = 0

Query: 173 GVLKPPPIEKQLECTLEELCFGCIKKIKVTRDVILNTGQAIEEEETVTIRVKPGWTKGTK 232
           G  K  P+EK+L C+LE+L  G  KK+K++R++    G+  + +E +T+ VKPGW  GTK
Sbjct: 103 GARKAAPVEKKLPCSLEDLYKGTTKKMKISREIAGVFGKTTQVQEILTVDVKPGWKTGTK 162

Query: 233 ITFEGMGNERPGSYPADVSFVIVEKQHLYFKREGDDLKLTVEIPLLKALTGCTISIPLLG 292
           ITF   GNE+PG  PAD+ F+I EK H  F REG+DL +T +I +L+A TG T+++  L 
Sbjct: 163 ITFSEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLVVTQKISVLEAFTGYTVNLTTLD 222

Query: 293 GDVMSLRTHDVIWHGCVKIIKGQGMPKLKEEANRGNLKVKFLIEFPTELTDQQRSDVCRI 352
           G  +++  + VI    V+++  +GMP  K++A +GNL++KF I+FPT LT +Q++ + ++
Sbjct: 223 GRRLTIPVNTVIHPEYVEVVPNEGMPLQKDQAKKGNLRIKFNIKFPTTLTSEQKTGLKKL 282

Query: 353 L 354
           L
Sbjct: 283 L 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6608044.13.2e-200100.00DnaJ-like subfamily B member 13, partial [Cucurbita argyrosperma subsp. sororia]... [more]
XP_022941087.11.6e-19698.61dnaJ homolog subfamily B member 5-like [Cucurbita moschata][more]
XP_023525805.12.9e-19397.21dnaJ homolog subfamily B member 5-like [Cucurbita pepo subsp. pepo][more]
XP_022981017.11.8e-19095.82dnaJ homolog subfamily B member 1-like [Cucurbita maxima][more]
XP_038899191.11.9e-14476.10dnaJ homolog subfamily B member 4-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q80Y757.1e-3341.18DnaJ homolog subfamily B member 13 OS=Mus musculus OX=10090 GN=Dnajb13 PE=1 SV=1[more]
P599102.1e-3241.18DnaJ homolog subfamily B member 13 OS=Homo sapiens OX=9606 GN=DNAJB13 PE=1 SV=1[more]
Q241331.3e-3142.37DnaJ protein homolog 1 OS=Drosophila melanogaster OX=7227 GN=DnaJ-1 PE=1 SV=3[more]
Q5BIP82.3e-3137.89DnaJ homolog subfamily B member 5 OS=Bos taurus OX=9913 GN=DNAJB5 PE=2 SV=1[more]
O759532.3e-3137.89DnaJ homolog subfamily B member 5 OS=Homo sapiens OX=9606 GN=DNAJB5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FLF58.0e-19798.61dnaJ homolog subfamily B member 5-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1ISU08.6e-19195.82dnaJ homolog subfamily B member 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114802... [more]
A0A1S3BAK81.9e-13473.30chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103487597 PE=4 SV=1[more]
A0A0A0KYJ44.9e-13071.47DnaJ_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361870 PE=4... [more]
A0A6J1BSE93.5e-12872.18uncharacterized protein LOC111005118 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
Match NameE-valueIdentityDescription
AT1G11040.17.7e-6749.08HSP40/DnaJ peptide-binding protein [more]
AT1G44160.11.7e-5842.52HSP40/DnaJ peptide-binding protein [more]
AT3G08910.12.7e-4341.71DNAJ heat shock family protein [more]
AT2G20560.11.5e-4144.20DNAJ heat shock family protein [more]
AT2G20550.13.2e-4143.09HSP40/DnaJ peptide-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.260.20coord: 176..258
e-value: 2.2E-28
score: 100.2
NoneNo IPR availableGENE3D2.60.260.20coord: 259..357
e-value: 9.0E-22
score: 79.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 107..171
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..79
NoneNo IPR availablePANTHERPTHR24078:SF535DNAJ HOMOLOG SUBFAMILY B MEMBER 13-LIKEcoord: 80..354
NoneNo IPR availablePANTHERPTHR24078DNAJ HOMOLOG SUBFAMILY C MEMBERcoord: 80..354
NoneNo IPR availableCDDcd10747DnaJ_Ccoord: 178..341
e-value: 1.65226E-50
score: 164.135
IPR002939Chaperone DnaJ, C-terminalPFAMPF01556DnaJ_Ccoord: 180..338
e-value: 4.1E-40
score: 137.4
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 179..259
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 261..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00330-RACarg00330-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0006457 protein folding
cellular_component GO:0005829 cytosol
molecular_function GO:0051087 chaperone binding
molecular_function GO:0051082 unfolded protein binding