Carg00311 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00311
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionglutathione S-transferase TCHQD-like
LocationCarg_Chr04: 6545582 .. 6548701 (+)
RNA-Seq ExpressionCarg00311
SyntenyCarg00311
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTTTTGTGTTCTTTGTTTCTTCTTCTTCTTCTTCACGTGCTCCCCACGAATCTGAAAAGAGATGTGTTTCTTTATCGATTTTCCTTTTGGTTTTCTTCTTGTTCTTCAGGTTTCCGAAGAATCGCATGGCTTCTGGCTCTCTGGTGTGAGCGTATGTTCTTATTTTGCCCTTAATTTCCTGTTTTAGGGTTCTTATTGAATCGTTTTTGGAATCTGATTGTGAAGATTATTATATCCGAGTCTTGTTTGCTTGTTTGTGTTCTTTACATGATTATGTAGAATCCTTTATATGAGGTACTTGATTTGATTATTTGAGTTTTGATGATTTCTTTGTCAAAGTAAAATAGGATTGTTTGATTACAAGAACTTCATGTTTTAGAAGATGGTTGTGGCTATCAAATTGTTGTTTCTGTTCACATTGTTGCATTGAATCTGAATGAGTGAAGTGTTGATCTGAAGTTCTTAAGAAGTGATGTTGCAGGCGGTATCTCGTTTTGTTTAGACATTGCTTAGCGTAAACTAGTATCGGGATCCGTTTTTTCTTGGTTCTTAGTGTAATAGTGTGAGATCTCACATTGGTTGGAGAGGGATACGAAGCATTGTTGATAAGGGTGTGGAAACCTCTTCCTAATAGATGTGTTGTAGATACCGTAAGGCTGAGGTGATACGTAATGGACCAAAATGGACAATATCTGTTAGTGGTGGGCTTGAGCTTTTACAAATGGTATCAGAGCCAGATACCGGGCGGTGTGCTAGTGAGGACGTTGGGTCCCTGAGGGGGGTGGATTGTGAGATCCCATATTAGTTGGAGATGGGAACGAAGCATGCTTATAAGGGTGTGGGAACCTCTCCCTAGTAGATGTGTTTTAAAAACTGTGAGGATAACGGCGATATGTAATGAGTCAAAACGGACGATGTTTGCTAGCGGTGGGCTTGAGCCGTTACAAATGGTATCAAAGCCAGACACTGGGTGGTGTGCTAGTGAGGATGCAGGGTCCCTAAGGGGGTGGATTGTGAGATCCCACATTGGTTGGAGATGGAAACGAAACGTTCCTTCTAAGGGTGTGGAAACCTCTCATTAGTAGACGTGTTTTAAAAACTGTGAGGCTAACAGTGATACGTAACGGACCAAAACGGACAAGAGTCAGACACCAGGCAGTGTGCTAGCAAGGACGCTGGGCTTCCAAGAGGGGTGGATTGTGAGATCCCACATCGGTTGAAGAGGGAAACGAAGCATTTCTTATAAGGGTGTGGACACCTCTTTCTAATAAACGTGTTTTAAAAACCGTGAGGCTGACGGCGCCAAAACGGAGACAGTATCTACTAGTGGTGGGCTTGGACGGTTACGATGAGTGTGCATATGAATAGGAACATCTTTGGCATATGAGTCTATGAGTTCCTCGTTTGATTTTCATCATCTTTCTATTATGGAAACTCGAGTTTTCTTTGATGTTGATTGCTTTCCTCAACGGTCATTGCAGGCTTTCGAGTCGACGTTTTCCTGTAGATCCCACGCGAACTTTTGAGCCATAAACCAAGAGCCAGTACTCCAAGTACTCTCTTGATGTTAGTAACTAAGCAATGCAGTTATATCATCATCCATACTCTTTGAACAGCCAGAAGGTGAGACTTGCCCTGGAAGAGCAAGGCATCGACTACACGTCGTATCATGTCAATCCTATCACGGCCAAGAACATGGACTCCTCGTTCTTCAAGATGAACCCGAGTGCAAAACTTCCCGTCTTTCAAAATGGTTCCCATGTCATTTTCGACACGATCGAGATAATTCAGTACGTCTTGCTTGCTCCTTACTCTTTTGTCCCTACACTTTTTATGTTCATGAGATTGGCTGGAATAACTCACATCATTCAGTTGAAGAGAGGGCGTGAAAACCTCTCTCTAACAAAAGCGTTTTAAAGTCACTAACGGTGATACGTAACGGGCCAAAGCGGACAATATCTGCTAGCGGTAGGCTTCGACTGTTACAAATGGTATCAGAGCCAGACACTGGGCGGTGTGCGAGCGAGGACGTTGAGCCACCAAGGAGGGTGGATTGTGAGATCCCATATCGGTTGGAGAGGGGAACAAAGCATTTTTTATAAGAGTGTGGAAACCTCTCCCTAACAAACGCATTTTACAACCGTGAGGCTGACGGTGATACGTAATAGGCCAAAGCGGATAATATCTGCTAGCAATGGCTTGGGCTGTTACAAATAGTATCAGAACCAGACACTGGACGGTGTGCCAACGAAGACGTTGGGCCCCCAAGTAGAGTGGATGGCGAGATCCCACGTCGGTTAGAGAGGGGAACAACATTCTTTACAAGGATATGGAAACCTTTCCCTAACAGACCCGTTTTAAAATCGTGAGGCTGATGACATTACGTAACGGGCCAAAGCATACAATATCTGCTAATGGTGGGCTTGGGCCAAAGTAGACAATATCTACTAACGGTCCAACCCGGCTATATATCTTTCTAAACTAATGTTCGTAATGTACTGATGGCTCTATTCAGGTATATAGAGAGAATTGCAGTTGTATCCTCGGGAACCAACGACGTAACGCCGAGTAGCCAAGAAGTTGTTGAATGGATGCATAAGATACAACAATGGAATCCAAAATTTTTCACTCTCGCCCACGTCCCCGAGAAGTACCGACTTACCGTTACCAAATTTATAAGGCGTGTCGTTATCAGTCGGATGGCCGAAACTCCTGAAATGGCAGCTGCATACCATCATAAGCTCAGAGAAGCATATGAGACAGAAGACAAACTGAAAAACAAAACTGTTATCAAACAAAGCACAGATCATTTAGTGAGACTTCTCGACGAAATCGAGTCGAAGCTAGGCGAAACTGCATTCTTGGCAGGGGAAGAGTTCACCATGGCTGATGCCGTGCTCATCCCGGTGCTGGCTCGACTCGAGCTGTTGAAGGTGGCGGACGAGTATATAGGCAGCAGCAGCAGCAGACCAAACATTGCAGAATATTGGATGTTGGTGCAGCAGAGGCCAAGCTATAAGAAGGTGATTGGAAAGTATTTCAAGGGATGGAGAAAGCACAAAACACTTATGAAAACATGGTGCTTTCTTCATATCAGAACCTTGCTAAGGAGATATTGA

mRNA sequence

GCTTTTGTGTTCTTTGTTTCTTCTTCTTCTTCTTCACGTGCTCCCCACGAATCTGAAAAGAGATGTGTTTCTTTATCGATTTTCCTTTTGGTTTTCTTCTTGTTCTTCAGGTTTCCGAAGAATCGCATGGCTTCTGGCTCTCTGGTGTGAGCGCTTTCGAGTCGACGTTTTCCTGTAGATCCCACGCGAACTTTTGAGCCATAAACCAAGAGCCAGTACTCCAAGTACTCTCTTGATGTTAGTAACTAAGCAATGCAGTTATATCATCATCCATACTCTTTGAACAGCCAGAAGGTGAGACTTGCCCTGGAAGAGCAAGGCATCGACTACACGTCGTATCATGTCAATCCTATCACGGCCAAGAACATGGACTCCTCGTTCTTCAAGATGAACCCGAGTGCAAAACTTCCCGTCTTTCAAAATGGTTCCCATGTCATTTTCGACACGATCGAGATAATTCACGTTTTAAAGTCACTAACGGTGATACGTAACGGGCCAAAGCGGACAATATCTGCTAGCGGTAGGCTTCGACTGTTACAAATGGTATCAGAGCCAGACACTGGGCGGTGTGCGAGCGAGGACGTTGAGCCACCAAGGAGGGTGGATTGTGAGATCCCATATCGGTATATAGAGAGAATTGCAGTTGTATCCTCGGGAACCAACGACGTAACGCCGAGTAGCCAAGAAGTTGTTGAATGGATGCATAAGATACAACAATGGAATCCAAAATTTTTCACTCTCGCCCACGTCCCCGAGAAGTACCGACTTACCGTTACCAAATTTATAAGGCGTGTCGTTATCAGTCGGATGGCCGAAACTCCTGAAATGGCAGCTGCATACCATCATAAGCTCAGAGAAGCATATGAGACAGAAGACAAACTGAAAAACAAAACTGTTATCAAACAAAGCACAGATCATTTAGTGAGACTTCTCGACGAAATCGAGTCGAAGCTAGGCGAAACTGCATTCTTGGCAGGGGAAGAGTTCACCATGGCTGATGCCGTGCTCATCCCGGTGCTGGCTCGACTCGAGCTGTTGAAGGTGGCGGACGAGTATATAGGCAGCAGCAGCAGCAGACCAAACATTGCAGAATATTGGATGTTGGTGCAGCAGAGGCCAAGCTATAAGAAGGTGATTGGAAAGTATTTCAAGGGATGGAGAAAGCACAAAACACTTATGAAAACATGGTGCTTTCTTCATATCAGAACCTTGCTAAGGAGATATTGA

Coding sequence (CDS)

ATGCAGTTATATCATCATCCATACTCTTTGAACAGCCAGAAGGTGAGACTTGCCCTGGAAGAGCAAGGCATCGACTACACGTCGTATCATGTCAATCCTATCACGGCCAAGAACATGGACTCCTCGTTCTTCAAGATGAACCCGAGTGCAAAACTTCCCGTCTTTCAAAATGGTTCCCATGTCATTTTCGACACGATCGAGATAATTCACGTTTTAAAGTCACTAACGGTGATACGTAACGGGCCAAAGCGGACAATATCTGCTAGCGGTAGGCTTCGACTGTTACAAATGGTATCAGAGCCAGACACTGGGCGGTGTGCGAGCGAGGACGTTGAGCCACCAAGGAGGGTGGATTGTGAGATCCCATATCGGTATATAGAGAGAATTGCAGTTGTATCCTCGGGAACCAACGACGTAACGCCGAGTAGCCAAGAAGTTGTTGAATGGATGCATAAGATACAACAATGGAATCCAAAATTTTTCACTCTCGCCCACGTCCCCGAGAAGTACCGACTTACCGTTACCAAATTTATAAGGCGTGTCGTTATCAGTCGGATGGCCGAAACTCCTGAAATGGCAGCTGCATACCATCATAAGCTCAGAGAAGCATATGAGACAGAAGACAAACTGAAAAACAAAACTGTTATCAAACAAAGCACAGATCATTTAGTGAGACTTCTCGACGAAATCGAGTCGAAGCTAGGCGAAACTGCATTCTTGGCAGGGGAAGAGTTCACCATGGCTGATGCCGTGCTCATCCCGGTGCTGGCTCGACTCGAGCTGTTGAAGGTGGCGGACGAGTATATAGGCAGCAGCAGCAGCAGACCAAACATTGCAGAATATTGGATGTTGGTGCAGCAGAGGCCAAGCTATAAGAAGGTGATTGGAAAGTATTTCAAGGGATGGAGAAAGCACAAAACACTTATGAAAACATGGTGCTTTCTTCATATCAGAACCTTGCTAAGGAGATATTGA

Protein sequence

MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSHVIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCEIPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRRVVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFLAGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFKGWRKHKTLMKTWCFLHIRTLLRRY
Homology
BLAST of Carg00311 vs. NCBI nr
Match: KAG7031654.1 (Glutathione S-transferase TCHQD [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 648.7 bits (1672), Expect = 2.7e-182
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH
Sbjct: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE
Sbjct: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
           IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR
Sbjct: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL
Sbjct: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTLMKTWCFLHIRTLLRRY
Sbjct: 301 GWRKHKTLMKTWCFLHIRTLLRRY 324

BLAST of Carg00311 vs. NCBI nr
Match: XP_023525288.1 (glutathione S-transferase TCHQD-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508.1 bits (1307), Expect = 5.7e-140
Identity = 266/324 (82.10%), Postives = 267/324 (82.41%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPS KLPVFQNGSH
Sbjct: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSVKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           VIFDTIEII                                                   
Sbjct: 61  VIFDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
              +YIERIAVVSS TNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR
Sbjct: 121 ---QYIERIAVVSSRTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL
Sbjct: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADAV IPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAVFIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 270

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTLMKTWCFLHIRTLLRRY
Sbjct: 301 GWRKHKTLMKTWCFLHIRTLLRRY 270

BLAST of Carg00311 vs. NCBI nr
Match: XP_022941031.1 (glutathione S-transferase TCHQD-like [Cucurbita moschata])

HSP 1 Score: 503.8 bits (1296), Expect = 1.1e-138
Identity = 265/324 (81.79%), Postives = 266/324 (82.10%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSLNSQKVRLALEE GID TSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH
Sbjct: 1   MQLYHHPYSLNSQKVRLALEEIGIDCTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           VIFDTIEII                                                   
Sbjct: 61  VIFDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
              +YIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR
Sbjct: 121 ---QYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIE KLGETAFL
Sbjct: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIELKLGETAFL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADAVLIPVLARLELLKVADEYIG SSSRPNIAEYWMLVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGGSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 270

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTLMKTWCFLHIRTLLRRY
Sbjct: 301 GWRKHKTLMKTWCFLHIRTLLRRY 270

BLAST of Carg00311 vs. NCBI nr
Match: XP_022981040.1 (glutathione S-transferase TCHQD-like [Cucurbita maxima])

HSP 1 Score: 502.3 bits (1292), Expect = 3.2e-138
Identity = 261/324 (80.56%), Postives = 266/324 (82.10%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPI AKNMDSSFFKMNPSAKLPVFQNGSH
Sbjct: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPIMAKNMDSSFFKMNPSAKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           VIFDTIEII                                                   
Sbjct: 61  VIFDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
              +YIERIAVVSSGT DV PS++EVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKF+RR
Sbjct: 121 ---QYIERIAVVSSGTEDVMPSNREVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFVRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           V+ISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL
Sbjct: 181 VIISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADAVLIPVLARLELLKVADEYIG SSSRPNIAEYWMLVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGGSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 270

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTLMKTWCFLHIRTLLRRY
Sbjct: 301 GWRKHKTLMKTWCFLHIRTLLRRY 270

BLAST of Carg00311 vs. NCBI nr
Match: XP_008444209.1 (PREDICTED: glutathione S-transferase TCHQD [Cucumis melo] >XP_008444210.1 PREDICTED: glutathione S-transferase TCHQD [Cucumis melo] >XP_008444211.1 PREDICTED: glutathione S-transferase TCHQD [Cucumis melo] >KAA0052227.1 glutathione S-transferase TCHQD [Cucumis melo var. makuwa] >TYK01791.1 glutathione S-transferase TCHQD [Cucumis melo var. makuwa])

HSP 1 Score: 466.5 bits (1199), Expect = 1.9e-127
Identity = 238/324 (73.46%), Postives = 260/324 (80.25%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSL+SQKVRLALEEQ IDYTSYHVNPITAKNMDSSFFK+NPSAKLPVFQNGSH
Sbjct: 1   MQLYHHPYSLDSQKVRLALEEQSIDYTSYHVNPITAKNMDSSFFKINPSAKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           +IFDTIEII                                                   
Sbjct: 61  IIFDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
              +YIERIA+VSSGT+D+ PSS+EVVEWMHKIQQWNPKFFTL+H+PEKYRLTVT+FIRR
Sbjct: 121 ---QYIERIAIVSSGTDDIMPSSREVVEWMHKIQQWNPKFFTLSHIPEKYRLTVTRFIRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVI+RMAETPEMAAAYH KLREAY+TEDKLKNK+V+KQSTDHLV LLDE+ESKL ET++L
Sbjct: 181 VVIARMAETPEMAAAYHRKLREAYDTEDKLKNKSVVKQSTDHLVALLDEVESKLSETSYL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADA+ IPVLARLELLK+ADEYIG   SRPNIAEYW LVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAIFIPVLARLELLKLADEYIG---SRPNIAEYWTLVQQRPSYKKVIGKYFK 267

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTL+KTWCF+HIRTLLRRY
Sbjct: 301 GWRKHKTLLKTWCFIHIRTLLRRY 267

BLAST of Carg00311 vs. ExPASy Swiss-Prot
Match: O80662 (Glutathione S-transferase TCHQD OS=Arabidopsis thaliana OX=3702 GN=TCHQD PE=2 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 8.2e-97
Identity = 184/324 (56.79%), Postives = 230/324 (70.99%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYS++SQ+VRLALEE+GIDYTSYHVNPIT K+MD SFF+MNP+AKLPVF+NGSH
Sbjct: 1   MQLYHHPYSIDSQRVRLALEEKGIDYTSYHVNPITGKHMDPSFFRMNPNAKLPVFRNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           +I DTIEII                                                   
Sbjct: 61  IILDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
               Y+ERIA VSSG  D T  ++EVVEWM KI++W  K FTLAH+P+  RL V+KF+R 
Sbjct: 121 ---EYLERIAEVSSGIEDAT-FNREVVEWMRKIREWESKLFTLAHIPDNRRLYVSKFLRM 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVI+RMAE+P++A+AYH KLREAY+TEDKLK+   +++S DHL+RLLDE+E+KL  T +L
Sbjct: 181 VVIARMAESPDLASAYHRKLREAYDTEDKLKDPGALRRSKDHLLRLLDEVETKLEGTTYL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AG EF+MAD +LIPVLARL LL + +EYI   SSR N+AEYW LV++RPSYKKVIG+YF 
Sbjct: 241 AGNEFSMADVMLIPVLARLSLLDLEEEYI---SSRKNLAEYWALVRRRPSYKKVIGRYFN 266

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRK+ TL+KTW F+ +R+LLR+Y
Sbjct: 301 GWRKYATLVKTWMFVRVRSLLRKY 266

BLAST of Carg00311 vs. ExPASy Swiss-Prot
Match: Q03520 (Tetrachloro-P-hydroquinone reductive dehalogenase OS=Sphingobium chlorophenolicum OX=46429 GN=pcpC PE=1 SV=3)

HSP 1 Score: 61.2 bits (147), Expect = 2.4e-08
Identity = 65/296 (21.96%), Postives = 117/296 (39.53%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVN-PITAKNMDSSFFKMNPSAKLPVFQNGS 60
           + LY++  S+ S K RLA+EE G+DY    V+     +N +  + ++N  A +P    G 
Sbjct: 4   VSLYNYTMSICSMKTRLAMEEFGVDYDDKQVDIGFALENFEPDYVRLNEKAVVPTLVVGD 63

Query: 61  HVIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDC 120
            V+ ++  I+                                         +E  +    
Sbjct: 64  RVVTNSYNIV-----------------------------------------LEAAKLGKV 123

Query: 121 EIPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAH--VPEKYRLTVTKF 180
            IP   +E               ++  ++W  K  Q N +  T  H  VP    L +   
Sbjct: 124 GIPADPVE---------------NKAALDWFQKGDQVNFQVITYGHKGVPRGDELLIA-- 183

Query: 181 IRRVVISRMAET-PEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGE 240
            RR      AE  PE+ + Y        E  +   +   + Q+   L + LDE+++ L +
Sbjct: 184 -RRERAKEYAEKYPELRSIYQAAHDRIVEHGNCAYDADTVAQAEVDLQKRLDELDAHLAD 236

Query: 241 TAFLAGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYK 293
             F+AG  +++AD +   +LAR+E+L +        S RPN+  Y+  ++ R S++
Sbjct: 244 KPFIAGSNYSIADIMWTVLLARIEMLNMT----AWISERPNLLAYYQRMKARRSFE 236

BLAST of Carg00311 vs. ExPASy Swiss-Prot
Match: P04907 (Glutathione S-transferase 3 OS=Zea mays OX=4577 GN=GST3 PE=1 SV=4)

HSP 1 Score: 50.1 bits (118), Expect = 5.6e-05
Identity = 25/83 (30.12%), Postives = 46/83 (55.42%), Query Frame = 0

Query: 215 VIKQSTDHLVRLLDEIESKLGETAFLAGEEFTMADA--VLIPVLARLELLKVADEYIGSS 274
           V+++  + L ++LD  E+ L    +LAG+EFT+ADA   L+P L             G  
Sbjct: 132 VVEKHAEQLAKVLDVYEAHLARNKYLAGDEFTLADANHALLPALT-----SARPPRPGCV 191

Query: 275 SSRPNIAEYWMLVQQRPSYKKVI 296
           ++RP++  +W  +  RP+++K +
Sbjct: 192 AARPHVKAWWEAIAARPAFQKTV 209

BLAST of Carg00311 vs. ExPASy Swiss-Prot
Match: Q9SRY5 (Glutathione S-transferase F7 OS=Arabidopsis thaliana OX=3702 GN=GSTF7 PE=2 SV=3)

HSP 1 Score: 50.1 bits (118), Expect = 5.6e-05
Identity = 30/87 (34.48%), Postives = 50/87 (57.47%), Query Frame = 0

Query: 212 NKTVIKQSTDHLVRLLDEIESKLGETAFLAGEEFTMADAVLIPVLARL---ELLKVADEY 271
           +KTV+++    L ++LD  E +LGE+ +LA ++FT+ D   IPV+  L      K+ DE 
Sbjct: 130 DKTVVEEEEAKLAKVLDVYEHRLGESKYLASDKFTLVDLHTIPVIQYLLGTPTKKLFDE- 189

Query: 272 IGSSSSRPNIAEYWMLVQQRPSYKKVI 296
                 RP+++ +   +  RPS KKV+
Sbjct: 190 ------RPHVSAWVADITSRPSAKKVL 209

BLAST of Carg00311 vs. ExPASy Swiss-Prot
Match: Q9LZI9 (Glutathione S-transferase F13 OS=Arabidopsis thaliana OX=3702 GN=GSTF13 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 7.3e-05
Identity = 28/105 (26.67%), Postives = 56/105 (53.33%), Query Frame = 0

Query: 190 PEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFLAGEEFTMAD 249
           P ++A  H  +    + E    N  +++++ ++L ++LD  E +LG+T +LAG+ +T+AD
Sbjct: 110 PAISAVIHQLIVVPLQGES--PNAAIVEENLENLGKILDVYEERLGKTKYLAGDTYTLAD 169

Query: 250 AVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKV 295
              +P         +   + G  + RPN+  +W  +  RP++ KV
Sbjct: 170 LHHVPYTYYF----MKTIHAGLINDRPNVKAWWEDLCSRPAFLKV 208

BLAST of Carg00311 vs. ExPASy TrEMBL
Match: A0A6J1FK12 (glutathione S-transferase TCHQD-like OS=Cucurbita moschata OX=3662 GN=LOC111446440 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 5.3e-139
Identity = 265/324 (81.79%), Postives = 266/324 (82.10%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSLNSQKVRLALEE GID TSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH
Sbjct: 1   MQLYHHPYSLNSQKVRLALEEIGIDCTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           VIFDTIEII                                                   
Sbjct: 61  VIFDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
              +YIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR
Sbjct: 121 ---QYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIE KLGETAFL
Sbjct: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIELKLGETAFL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADAVLIPVLARLELLKVADEYIG SSSRPNIAEYWMLVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGGSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 270

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTLMKTWCFLHIRTLLRRY
Sbjct: 301 GWRKHKTLMKTWCFLHIRTLLRRY 270

BLAST of Carg00311 vs. ExPASy TrEMBL
Match: A0A6J1IYB9 (glutathione S-transferase TCHQD-like OS=Cucurbita maxima OX=3661 GN=LOC111480307 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.5e-138
Identity = 261/324 (80.56%), Postives = 266/324 (82.10%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPI AKNMDSSFFKMNPSAKLPVFQNGSH
Sbjct: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPIMAKNMDSSFFKMNPSAKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           VIFDTIEII                                                   
Sbjct: 61  VIFDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
              +YIERIAVVSSGT DV PS++EVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKF+RR
Sbjct: 121 ---QYIERIAVVSSGTEDVMPSNREVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFVRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           V+ISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL
Sbjct: 181 VIISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADAVLIPVLARLELLKVADEYIG SSSRPNIAEYWMLVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGGSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 270

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTLMKTWCFLHIRTLLRRY
Sbjct: 301 GWRKHKTLMKTWCFLHIRTLLRRY 270

BLAST of Carg00311 vs. ExPASy TrEMBL
Match: A0A1S3BAL6 (glutathione S-transferase TCHQD OS=Cucumis melo OX=3656 GN=LOC103487615 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 9.3e-128
Identity = 238/324 (73.46%), Postives = 260/324 (80.25%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSL+SQKVRLALEEQ IDYTSYHVNPITAKNMDSSFFK+NPSAKLPVFQNGSH
Sbjct: 1   MQLYHHPYSLDSQKVRLALEEQSIDYTSYHVNPITAKNMDSSFFKINPSAKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           +IFDTIEII                                                   
Sbjct: 61  IIFDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
              +YIERIA+VSSGT+D+ PSS+EVVEWMHKIQQWNPKFFTL+H+PEKYRLTVT+FIRR
Sbjct: 121 ---QYIERIAIVSSGTDDIMPSSREVVEWMHKIQQWNPKFFTLSHIPEKYRLTVTRFIRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVI+RMAETPEMAAAYH KLREAY+TEDKLKNK+V+KQSTDHLV LLDE+ESKL ET++L
Sbjct: 181 VVIARMAETPEMAAAYHRKLREAYDTEDKLKNKSVVKQSTDHLVALLDEVESKLSETSYL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADA+ IPVLARLELLK+ADEYIG   SRPNIAEYW LVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAIFIPVLARLELLKLADEYIG---SRPNIAEYWTLVQQRPSYKKVIGKYFK 267

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTL+KTWCF+HIRTLLRRY
Sbjct: 301 GWRKHKTLLKTWCFIHIRTLLRRY 267

BLAST of Carg00311 vs. ExPASy TrEMBL
Match: A0A5A7UCM6 (Glutathione S-transferase TCHQD OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G00230 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 9.3e-128
Identity = 238/324 (73.46%), Postives = 260/324 (80.25%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSL+SQKVRLALEEQ IDYTSYHVNPITAKNMDSSFFK+NPSAKLPVFQNGSH
Sbjct: 1   MQLYHHPYSLDSQKVRLALEEQSIDYTSYHVNPITAKNMDSSFFKINPSAKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           +IFDTIEII                                                   
Sbjct: 61  IIFDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
              +YIERIA+VSSGT+D+ PSS+EVVEWMHKIQQWNPKFFTL+H+PEKYRLTVT+FIRR
Sbjct: 121 ---QYIERIAIVSSGTDDIMPSSREVVEWMHKIQQWNPKFFTLSHIPEKYRLTVTRFIRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVI+RMAETPEMAAAYH KLREAY+TEDKLKNK+V+KQSTDHLV LLDE+ESKL ET++L
Sbjct: 181 VVIARMAETPEMAAAYHRKLREAYDTEDKLKNKSVVKQSTDHLVALLDEVESKLSETSYL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADA+ IPVLARLELLK+ADEYIG   SRPNIAEYW LVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAIFIPVLARLELLKLADEYIG---SRPNIAEYWTLVQQRPSYKKVIGKYFK 267

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTL+KTWCF+HIRTLLRRY
Sbjct: 301 GWRKHKTLLKTWCFIHIRTLLRRY 267

BLAST of Carg00311 vs. ExPASy TrEMBL
Match: A0A0A0KYI5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G358760 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 7.9e-127
Identity = 238/324 (73.46%), Postives = 260/324 (80.25%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYSL+SQKVRLALEEQ IDYTSYHVNPITAKNMDSSFFK+NPSAKLPVFQNGSH
Sbjct: 1   MQLYHHPYSLDSQKVRLALEEQSIDYTSYHVNPITAKNMDSSFFKINPSAKLPVFQNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           +IFDTIEII                                                   
Sbjct: 61  IIFDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
              +YIERIAVVSSGT+++ PSS+EVVEWMHKIQ+WNPK+FTLAH+PEKYRLTVT+FIRR
Sbjct: 121 ---QYIERIAVVSSGTDEIMPSSREVVEWMHKIQEWNPKYFTLAHIPEKYRLTVTRFIRR 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVISRMAETPEMAAAYH KLREAYETEDKLKNK+V+KQSTDHLV LLDEIE KL ET+++
Sbjct: 181 VVISRMAETPEMAAAYHRKLREAYETEDKLKNKSVVKQSTDHLVALLDEIELKLSETSYI 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AGEEFTMADAV IPVLARLELLK+ADEYIG   SRPNI+EYW+LVQQRPSYKKVIGKYFK
Sbjct: 241 AGEEFTMADAVFIPVLARLELLKLADEYIG---SRPNISEYWILVQQRPSYKKVIGKYFK 267

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRKHKTL+KTWCF+HIRTLLRRY
Sbjct: 301 GWRKHKTLLKTWCFVHIRTLLRRY 267

BLAST of Carg00311 vs. TAIR 10
Match: AT1G77290.1 (Glutathione S-transferase family protein )

HSP 1 Score: 355.1 bits (910), Expect = 5.8e-98
Identity = 184/324 (56.79%), Postives = 230/324 (70.99%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYS++SQ+VRLALEE+GIDYTSYHVNPIT K+MD SFF+MNP+AKLPVF+NGSH
Sbjct: 1   MQLYHHPYSIDSQRVRLALEEKGIDYTSYHVNPITGKHMDPSFFRMNPNAKLPVFRNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           +I DTIEII                                                   
Sbjct: 61  IILDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
               Y+ERIA VSSG  D T  ++EVVEWM KI++W  K FTLAH+P+  RL V+KF+R 
Sbjct: 121 ---EYLERIAEVSSGIEDAT-FNREVVEWMRKIREWESKLFTLAHIPDNRRLYVSKFLRM 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVI+RMAE+P++A+AYH KLREAY+TEDKLK+   +++S DHL+RLLDE+E+KL  T +L
Sbjct: 181 VVIARMAESPDLASAYHRKLREAYDTEDKLKDPGALRRSKDHLLRLLDEVETKLEGTTYL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AG EF+MAD +LIPVLARL LL + +EYI   SSR N+AEYW LV++RPSYKKVIG+YF 
Sbjct: 241 AGNEFSMADVMLIPVLARLSLLDLEEEYI---SSRKNLAEYWALVRRRPSYKKVIGRYFN 266

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRK+ TL+KTW F+ +R+LLR+Y
Sbjct: 301 GWRKYATLVKTWMFVRVRSLLRKY 266

BLAST of Carg00311 vs. TAIR 10
Match: AT1G77290.2 (Glutathione S-transferase family protein )

HSP 1 Score: 355.1 bits (910), Expect = 5.8e-98
Identity = 184/324 (56.79%), Postives = 230/324 (70.99%), Query Frame = 0

Query: 1   MQLYHHPYSLNSQKVRLALEEQGIDYTSYHVNPITAKNMDSSFFKMNPSAKLPVFQNGSH 60
           MQLYHHPYS++SQ+VRLALEE+GIDYTSYHVNPIT K+MD SFF+MNP+AKLPVF+NGSH
Sbjct: 1   MQLYHHPYSIDSQRVRLALEEKGIDYTSYHVNPITGKHMDPSFFRMNPNAKLPVFRNGSH 60

Query: 61  VIFDTIEIIHVLKSLTVIRNGPKRTISASGRLRLLQMVSEPDTGRCASEDVEPPRRVDCE 120
           +I DTIEII                                                   
Sbjct: 61  IILDTIEII--------------------------------------------------- 120

Query: 121 IPYRYIERIAVVSSGTNDVTPSSQEVVEWMHKIQQWNPKFFTLAHVPEKYRLTVTKFIRR 180
               Y+ERIA VSSG  D T  ++EVVEWM KI++W  K FTLAH+P+  RL V+KF+R 
Sbjct: 121 ---EYLERIAEVSSGIEDAT-FNREVVEWMRKIREWESKLFTLAHIPDNRRLYVSKFLRM 180

Query: 181 VVISRMAETPEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFL 240
           VVI+RMAE+P++A+AYH KLREAY+TEDKLK+   +++S DHL+RLLDE+E+KL  T +L
Sbjct: 181 VVIARMAESPDLASAYHRKLREAYDTEDKLKDPGALRRSKDHLLRLLDEVETKLEGTTYL 240

Query: 241 AGEEFTMADAVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKVIGKYFK 300
           AG EF+MAD +LIPVLARL LL + +EYI   SSR N+AEYW LV++RPSYKKVIG+YF 
Sbjct: 241 AGNEFSMADVMLIPVLARLSLLDLEEEYI---SSRKNLAEYWALVRRRPSYKKVIGRYFN 266

Query: 301 GWRKHKTLMKTWCFLHIRTLLRRY 325
           GWRK+ TL+KTW F+ +R+LLR+Y
Sbjct: 301 GWRKYATLVKTWMFVRVRSLLRKY 266

BLAST of Carg00311 vs. TAIR 10
Match: AT1G02920.1 (glutathione S-transferase 7 )

HSP 1 Score: 50.1 bits (118), Expect = 4.0e-06
Identity = 30/87 (34.48%), Postives = 50/87 (57.47%), Query Frame = 0

Query: 212 NKTVIKQSTDHLVRLLDEIESKLGETAFLAGEEFTMADAVLIPVLARL---ELLKVADEY 271
           +KTV+++    L ++LD  E +LGE+ +LA ++FT+ D   IPV+  L      K+ DE 
Sbjct: 130 DKTVVEEEEAKLAKVLDVYEHRLGESKYLASDKFTLVDLHTIPVIQYLLGTPTKKLFDE- 189

Query: 272 IGSSSSRPNIAEYWMLVQQRPSYKKVI 296
                 RP+++ +   +  RPS KKV+
Sbjct: 190 ------RPHVSAWVADITSRPSAKKVL 209

BLAST of Carg00311 vs. TAIR 10
Match: AT3G62760.1 (Glutathione S-transferase family protein )

HSP 1 Score: 49.7 bits (117), Expect = 5.2e-06
Identity = 28/105 (26.67%), Postives = 56/105 (53.33%), Query Frame = 0

Query: 190 PEMAAAYHHKLREAYETEDKLKNKTVIKQSTDHLVRLLDEIESKLGETAFLAGEEFTMAD 249
           P ++A  H  +    + E    N  +++++ ++L ++LD  E +LG+T +LAG+ +T+AD
Sbjct: 110 PAISAVIHQLIVVPLQGES--PNAAIVEENLENLGKILDVYEERLGKTKYLAGDTYTLAD 169

Query: 250 AVLIPVLARLELLKVADEYIGSSSSRPNIAEYWMLVQQRPSYKKV 295
              +P         +   + G  + RPN+  +W  +  RP++ KV
Sbjct: 170 LHHVPYTYYF----MKTIHAGLINDRPNVKAWWEDLCSRPAFLKV 208

BLAST of Carg00311 vs. TAIR 10
Match: AT1G02930.1 (glutathione S-transferase 6 )

HSP 1 Score: 48.1 bits (113), Expect = 1.5e-05
Identity = 29/87 (33.33%), Postives = 49/87 (56.32%), Query Frame = 0

Query: 212 NKTVIKQSTDHLVRLLDEIESKLGETAFLAGEEFTMADAVLIPVLARL---ELLKVADEY 271
           +KTV+++    L ++LD  E +LGE+ +LA + FT+ D   IPV+  L      K+ DE 
Sbjct: 129 DKTVVEEEEAKLAKVLDVYEHRLGESKYLASDHFTLVDLHTIPVIQYLLGTPTKKLFDE- 188

Query: 272 IGSSSSRPNIAEYWMLVQQRPSYKKVI 296
                 RP+++ +   +  RPS +KV+
Sbjct: 189 ------RPHVSAWVADITSRPSAQKVL 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031654.12.7e-182100.00Glutathione S-transferase TCHQD [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023525288.15.7e-14082.10glutathione S-transferase TCHQD-like [Cucurbita pepo subsp. pepo][more]
XP_022941031.11.1e-13881.79glutathione S-transferase TCHQD-like [Cucurbita moschata][more]
XP_022981040.13.2e-13880.56glutathione S-transferase TCHQD-like [Cucurbita maxima][more]
XP_008444209.11.9e-12773.46PREDICTED: glutathione S-transferase TCHQD [Cucumis melo] >XP_008444210.1 PREDIC... [more]
Match NameE-valueIdentityDescription
O806628.2e-9756.79Glutathione S-transferase TCHQD OS=Arabidopsis thaliana OX=3702 GN=TCHQD PE=2 SV... [more]
Q035202.4e-0821.96Tetrachloro-P-hydroquinone reductive dehalogenase OS=Sphingobium chlorophenolicu... [more]
P049075.6e-0530.12Glutathione S-transferase 3 OS=Zea mays OX=4577 GN=GST3 PE=1 SV=4[more]
Q9SRY55.6e-0534.48Glutathione S-transferase F7 OS=Arabidopsis thaliana OX=3702 GN=GSTF7 PE=2 SV=3[more]
Q9LZI97.3e-0526.67Glutathione S-transferase F13 OS=Arabidopsis thaliana OX=3702 GN=GSTF13 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1FK125.3e-13981.79glutathione S-transferase TCHQD-like OS=Cucurbita moschata OX=3662 GN=LOC1114464... [more]
A0A6J1IYB91.5e-13880.56glutathione S-transferase TCHQD-like OS=Cucurbita maxima OX=3661 GN=LOC111480307... [more]
A0A1S3BAL69.3e-12873.46glutathione S-transferase TCHQD OS=Cucumis melo OX=3656 GN=LOC103487615 PE=4 SV=... [more]
A0A5A7UCM69.3e-12873.46Glutathione S-transferase TCHQD OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0KYI57.9e-12773.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G358760 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G77290.15.8e-9856.79Glutathione S-transferase family protein [more]
AT1G77290.25.8e-9856.79Glutathione S-transferase family protein [more]
AT1G02920.14.0e-0634.48glutathione S-transferase 7 [more]
AT3G62760.15.2e-0626.67Glutathione S-transferase family protein [more]
AT1G02930.11.5e-0533.33glutathione S-transferase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 1..75
e-value: 7.3E-16
score: 60.3
NoneNo IPR availableGENE3D1.20.1050.10coord: 180..295
e-value: 3.6E-17
score: 64.1
NoneNo IPR availablePFAMPF13410GST_C_2coord: 215..281
e-value: 1.4E-9
score: 37.8
NoneNo IPR availableCDDcd00299GST_C_familycoord: 194..282
e-value: 6.25122E-12
score: 59.4347
NoneNo IPR availableCDDcd00570GST_N_familycoord: 1..70
e-value: 1.1995E-14
score: 66.056
IPR004045Glutathione S-transferase, N-terminalPFAMPF13417GST_N_3coord: 3..74
e-value: 6.7E-12
score: 45.6
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 1..80
score: 16.984058
IPR044617Tetrachlorohydroquinone dehalogenasePANTHERPTHR45374GLUTATHIONE S-TRANSFERASE TCHQDcoord: 1..83
coord: 123..324
IPR010987Glutathione S-transferase, C-terminal-likePROSITEPS50405GST_CTERcoord: 172..308
score: 11.745157
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 191..299
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 1..72

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00311-RACarg00311-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006749 glutathione metabolic process
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding