Carg00166 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00166
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Chalcone-flavonone isomerase family protein
Location	Carg_Chr04: 5705321 .. 5708153 (+)
RNA-Seq Expression	Carg00166
Synteny	Carg00166

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGCAGCAAAATTAAAGCGCCAGACCAAACAAGTCGCGGCCCGTGTCCTTTCTGCGCTACCTACTCTGCTCTGCCTCGGCATAGACATCGACATCGACATCGACGGAGTCCTATTTATAGCGTCTAATTCCAACTCCATGATCTCACATCCTTCACCGTCAAGCCTATTACCGCTCTGCTGCTGAAGAACTAACTTCCTTTCGTTTCTTCATCTGTTTTCCTTTCCCATCACGATCGCGATCAGTAAGGCCTTCTCTCCTTCTTTCTGTTCTCGTTTTCGTGCCTCTCAACTGTTTGTGTTAATGTTTCAATGAAAAACTGCAGCGATTTCACTTGAAAATAGACTCTATTCTGCTAGATTTGGGCATTTTGTTTGTCTGAATTCGATAGATTGATCGATTTGTTGCGTTTTGTGTATCTGAACTTAGGATTTTCTTTTTCTTTCTTTTGATTTGTGCGTTGGCGTTGGAATTTTGTGAACAGTGGGGAGTGAAGTGGAAGTGGTAGCGGACATTGCTTTCCCTAACCAGATCGTTAAGAACAAGCCGTTGTCTCTGTTGGGTCATGGTGAGTCCATACTTTTTCATTTTTCATCATTTTTAATATCTTTTTTCGCTATATTACAAAATATGATGAGAATAATATTATCCAAACTTTTAAAGAATTTATATTTCACTAATATTTTTGAAACATTTATACCACTTTTAAGAAAATATTAGTATTTTAAAAGAATATAGAAATATAAATTAAGGATTTAGAACTCCTTTTTAACTTATGAGATGGTTATCGATGCTTTAATTGTGTGATCTCTATAATTTTGAAAGTATTATTTTATTTTTAATTATAAATATAAAAAATATATTATTAATATTGATAGATAAGTCAAAAGTGAAATATTTTTGAAAGAGATGTGATAGAGATGTATTATGATTTAGCCATTCAAGCTTTTGTGGGTTTTTTTTTTTTGTTATTAATTATTTTTTAGTTTTTCATTCTTTTAAAAAATTAGAGAGATGAAGAAAGGTTTAGAACAGAGGCATGGAAATAATAGTTTAGATACATTAAGATTTTAAAAAATGACTGTTAAATCACTAATAGCGAAGTTCGTGGATATATTACAGGAATTATCGACCTCGAGATTCACTTTCTACAAATAAAATTCACGGCCATTGGAGTTTATCTGGAGCCTGCGATTGTGGAGCATTTGAAGCAATGGAAGGGAAAAGCCGCTGAGGACTTGCTGAAAGATGATGACTTCTTTGAGGCCATTGTTTCTGGTATCTCCCTAATTTTCCTATTTAACTATATACACCAATTATAATACTAAAAGTAATGCCAAAAAAAGCGAGTATAATTTAGCTAGTTGGTCGAGCAATAGTTTTTGGTATAATAAAACGGACGAGTTGATCTATTCAAGTAGTCGAGAATGTTAGATGAACACGACTCTCCATAATGGTATGATATGTTCTACTTTGAGTCCCAAAAGGTCTCACACTAATGGAGAGTGTGAGTATTCCTTGATTATAACCCATGATCATTCTCTAAATTAGCCAATGTGGACTTTGATCCAACACCTCCCCTTGAACAAAGTGGAGTCTTAGTCATTTTTTATTATACCTTCTAGGGGCCTCGACTTTTGGAGTCATTTGTTCGACGTTTGGGGATTCTATTGACATGGCTAATTTAGGGCATGACTCTAATATCATGTTAGATGAACACGACTCTCCACAATAGTATGATATAGTCCACTTTGAGCATAGGGCATGGCTCTGATACCATGTTAGATGAACACGACTCTCCACAATAGTATGATATAGTCCACTTTGAGCATAAACTCTCATGGCTTTGTTTTAGGCTTCCCCAAAATGCCCCACACCAATGGAGAGAGTATTCTTGGGTTTATAAACCCATGATCATTCTCTAAATTAGCTACGTGAGACTTTCATCCAATAGGGAAATTGTTTGATGAGTGTTTTTGTTTTCAAAACTTCTCATGATTGTCGTGAGCGTTGAAATCTGGATGAGTTGATCTATGTAAAGATTCGAAAAGTTGTATGGTGCGTGTTCTTGTTTTTGAAATATCTCATAAAAGTTAAAAGAATATTTTGAAAGCAGATTCCAAACAAGATGTTGTGTTTTATGTTCTTGATTTGCTTTCTTATGTTCATATACTCCTCTTTATTTTCGACTCGTAACATGAATCGGGAAACCTAAACCTTAAACACTCGAGTGAATTTGAATGGTTACAGCTCCCGTGGAGAAGGCTATAAGAGTTGTGGTGATCAAAGAGATCAAAGGGGCACAATATGGGTTGCAGTTGGAGAATGCAGTTAGGGATAGATTGGCTGCCGATGACAAATACGAGGAAGAAGAGGAAGCTCAGCTGGAAAAAGTTGTTGAATTCTTTCGGTCCAAATACTTGAGCTCCCATTCTGTTATAAGTTTCCATTTCCCATCAGCTCCGACCATAGCTGAGGTAACAAACTTCAACTAAACCCGAACAATTAGATTGGATCACATAGTTTCTGAAAAATTGTATGTTTTATATTCGTTGCAGATTGAGTTTTCGAGCAATGAGAAAGAGGGGTCGAAGATGAAAGTAGAGAATGGGAATGTGGTTGAGATGATTAAGAAATGGTACCTGGGAGGGACCAGAGGTGCGTCTCAAACAACCATATCTTCATTGGCTAAGAATCTTGCCATTGAGTTATCTAAATGAACCCAATTGCCTGCCCCTCTACATAGCTTTGGAGTGCTAGCTCAATGCTTATTAATGGTGCTTTCTATGCTTTGCTAATCCTTCTTCTTGAAGAACTATCACATTTCTAGTACA

mRNA sequence

CAGCAGCAAAATTAAAGCGCCAGACCAAACAAGTCGCGGCCCGTGTCCTTTCTGCGCTACCTACTCTGCTCTGCCTCGGCATAGACATCGACATCGACATCGACGGAGTCCTATTTATAGCGTCTAATTCCAACTCCATGATCTCACATCCTTCACCGTCAAGCCTATTACCGCTCTGCTGCTGAAGAACTAACTTCCTTTCGTTTCTTCATCTGTTTTCCTTTCCCATCACGATCGCGATCATGGGGAGTGAAGTGGAAGTGGTAGCGGACATTGCTTTCCCTAACCAGATCGTTAAGAACAAGCCGTTGTCTCTGTTGGGTCATGGAATTATCGACCTCGAGATTCACTTTCTACAAATAAAATTCACGGCCATTGGAGTTTATCTGGAGCCTGCGATTGTGGAGCATTTGAAGCAATGGAAGGGAAAAGCCGCTGAGGACTTGCTGAAAGATGATGACTTCTTTGAGGCCATTGTTTCTGCTCCCGTGGAGAAGGCTATAAGAGTTGTGGTGATCAAAGAGATCAAAGGGGCACAATATGGGTTGCAGTTGGAGAATGCAGTTAGGGATAGATTGGCTGCCGATGACAAATACGAGGAAGAAGAGGAAGCTCAGCTGGAAAAAGTTGTTGAATTCTTTCGGTCCAAATACTTGAGCTCCCATTCTGTTATAAGTTTCCATTTCCCATCAGCTCCGACCATAGCTGAGATTGAGTTTTCGAGCAATGAGAAAGAGGGGTCGAAGATGAAAGTAGAGAATGGGAATGTGGTTGAGATGATTAAGAAATGGTACCTGGGAGGGACCAGAGGTGCGTCTCAAACAACCATATCTTCATTGGCTAAGAATCTTGCCATTGAGTTATCTAAATGAACCCAATTGCCTGCCCCTCTACATAGCTTTGGAGTGCTAGCTCAATGCTTATTAATGGTGCTTTCTATGCTTTGCTAATCCTTCTTCTTGAAGAACTATCACATTTCTAGTACA

Coding sequence (CDS)

ATGGGGAGTGAAGTGGAAGTGGTAGCGGACATTGCTTTCCCTAACCAGATCGTTAAGAACAAGCCGTTGTCTCTGTTGGGTCATGGAATTATCGACCTCGAGATTCACTTTCTACAAATAAAATTCACGGCCATTGGAGTTTATCTGGAGCCTGCGATTGTGGAGCATTTGAAGCAATGGAAGGGAAAAGCCGCTGAGGACTTGCTGAAAGATGATGACTTCTTTGAGGCCATTGTTTCTGCTCCCGTGGAGAAGGCTATAAGAGTTGTGGTGATCAAAGAGATCAAAGGGGCACAATATGGGTTGCAGTTGGAGAATGCAGTTAGGGATAGATTGGCTGCCGATGACAAATACGAGGAAGAAGAGGAAGCTCAGCTGGAAAAAGTTGTTGAATTCTTTCGGTCCAAATACTTGAGCTCCCATTCTGTTATAAGTTTCCATTTCCCATCAGCTCCGACCATAGCTGAGATTGAGTTTTCGAGCAATGAGAAAGAGGGGTCGAAGATGAAAGTAGAGAATGGGAATGTGGTTGAGATGATTAAGAAATGGTACCTGGGAGGGACCAGAGGTGCGTCTCAAACAACCATATCTTCATTGGCTAAGAATCTTGCCATTGAGTTATCTAAATGA

Protein sequence

MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQWKGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEEEEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMIKKWYLGGTRGASQTTISSLAKNLAIELSK

Homology

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BLAST of Carg00166 vs. NCBI nr
Match: KAG7031511.1 (putative chalcone--flavonone isomerase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 401.4 bits (1030), Expect = 4.9e-108
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW
Sbjct: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE
Sbjct: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI
Sbjct: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRGASQTTISSLAKNLAIELSK
Sbjct: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 209

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BLAST of Carg00166 vs. NCBI nr
Match: KAG6600877.1 (putative chalcone--flavonone isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 399.4 bits (1025), Expect = 1.9e-107
Identity = 208/209 (99.52%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW
Sbjct: 22  MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 81

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAA+DKYEE
Sbjct: 82  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAANDKYEE 141

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI
Sbjct: 142 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 201

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRGASQTTISSLAKNLAIELSK
Sbjct: 202 KKWYLGGTRGASQTTISSLAKNLAIELSK 230

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BLAST of Carg00166 vs. NCBI nr
Match: XP_022943122.1 (probable chalcone--flavonone isomerase 3 [Cucurbita moschata])

HSP 1 Score: 395.2 bits (1014), Expect = 3.5e-106
Identity = 206/209 (98.56%), Postives = 207/209 (99.04%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW
Sbjct: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           KG AAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE
Sbjct: 61  KGNAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEF S+EKEGSKMKVENGNVVEMI
Sbjct: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFLSDEKEGSKMKVENGNVVEMI 180

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRGASQTTISSLAKNLAIELSK
Sbjct: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 209

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BLAST of Carg00166 vs. NCBI nr
Match: XP_023527694.1 (probable chalcone--flavonone isomerase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387.1 bits (993), Expect = 9.5e-104
Identity = 202/209 (96.65%), Postives = 204/209 (97.61%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           MGSEVEVVADIAFP QIV+NKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW
Sbjct: 1   MGSEVEVVADIAFPKQIVQNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE
Sbjct: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEKVVEFFRSKYLS+HSVISFHFPSAPTIAEIEFSS  KEGSKMKVENGNVVEMI
Sbjct: 121 EEEAQLEKVVEFFRSKYLSAHSVISFHFPSAPTIAEIEFSSYGKEGSKMKVENGNVVEMI 180

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRG SQTTISSLA NLAIELSK
Sbjct: 181 KKWYLGGTRGVSQTTISSLANNLAIELSK 209

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BLAST of Carg00166 vs. NCBI nr
Match: XP_022992837.1 (probable chalcone--flavonone isomerase 3 [Cucurbita maxima])

HSP 1 Score: 379.8 bits (974), Expect = 1.5e-101
Identity = 196/209 (93.78%), Postives = 202/209 (96.65%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           MGSEVEVVADIAFP QIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW
Sbjct: 1   MGSEVEVVADIAFPKQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           +GKAAE LLKDDDFFEAIVSAPVEKA+RV VIKEIKGAQY LQLENAVRDRLAADDKYEE
Sbjct: 61  EGKAAEGLLKDDDFFEAIVSAPVEKAVRVAVIKEIKGAQYALQLENAVRDRLAADDKYEE 120

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEK+VEFFRSKYLS+HSVISFHFPSAPTIAEIEFSS+ KEGSKMKVENGNVVEMI
Sbjct: 121 EEEAQLEKIVEFFRSKYLSAHSVISFHFPSAPTIAEIEFSSDGKEGSKMKVENGNVVEMI 180

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRG SQTTISSLA NLAI+LSK
Sbjct: 181 KKWYLGGTRGVSQTTISSLANNLAIQLSK 209

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BLAST of Carg00166 vs. ExPASy Swiss-Prot
Match: A0A2U7XUE3 (Chalcone isomerase-like protein 2 OS=Humulus lupulus OX=3486 GN=CHIL2 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 5.0e-71
Identity = 134/209 (64.11%), Postives = 169/209 (80.86%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           M + + +V +I FP +I   KPLSLLG+GI D+EIHFLQ+KFTAIGVYL+  +V+HL+QW
Sbjct: 1   MENNMVMVHEIPFPPEIKTTKPLSLLGYGITDMEIHFLQVKFTAIGVYLDSDVVKHLQQW 60

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           KGK   +L +DDDFF+A++SAPVEK +R+VVIKEIKG+QYG+QLE++VRDRLAA+D YEE
Sbjct: 61  KGKKGNELAEDDDFFDALISAPVEKYLRIVVIKEIKGSQYGVQLESSVRDRLAAEDMYEE 120

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEA LEK+VEF +SKY    ++I+FHFP+    AEI  +   KE SK+KVEN NVV+MI
Sbjct: 121 EEEAALEKIVEFLQSKYFKKDTLITFHFPATSPTAEIVVTLEGKEESKLKVENKNVVDMI 180

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGT G S +TISSLA NL+ ELSK
Sbjct: 181 KKWYLGGTSGVSPSTISSLADNLSAELSK 209

[Close]

BLAST of Carg00166 vs. ExPASy Swiss-Prot
Match: Q8VZW3 (Probable chalcone--flavanone isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=CHI3 PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 2.8e-66
Identity = 127/209 (60.77%), Postives = 165/209 (78.95%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVE-HLKQ 60
           MG+E+ +V ++ FP QI+ +KPLSLLG GI D+EIHFLQ+KFTAIGVYL+P+ V+ HL  
Sbjct: 1   MGTEMVMVHEVPFPPQIITSKPLSLLGQGITDIEIHFLQVKFTAIGVYLDPSDVKTHLDN 60

Query: 61  WKGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYE 120
           WKGK  ++L  DDDFF+A+ SA +EK IRVVVIKEIKGAQYG+QLEN VRDRLA +DKYE
Sbjct: 61  WKGKTGKELAGDDDFFDALASAEMEKVIRVVVIKEIKGAQYGVQLENTVRDRLAEEDKYE 120

Query: 121 EEEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEM 180
           EEEE +LEKVV FF+SKY  ++SVI++HF +   I EI F +  KE  K+KVEN NVV M
Sbjct: 121 EEEETELEKVVGFFQSKYFKANSVITYHFSAKDGICEIGFETEGKEEEKLKVENANVVGM 180

Query: 181 IKKWYLGGTRGASQTTISSLAKNLAIELS 209
           +++WYL G+RG S +TI S+A +++  L+
Sbjct: 181 MQRWYLSGSRGVSPSTIVSIADSISAVLT 209

[Close]

BLAST of Carg00166 vs. ExPASy Swiss-Prot
Match: Q3Y4F4 (Chalcone--flavanone isomerase OS=Canna generalis OX=341692 GN=CHI PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-13
Identity = 61/175 (34.86%), Postives = 83/175 (47.43%), Query Frame = 0


Query: 7   VVADIAFPNQIV---KNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHL-KQWKG 66
           V+ ++ FP        +  L L G G   LEI    + FTAIGVYLE   V  L  +WKG
Sbjct: 12  VIGEVTFPPAAAPPGSSSSLFLAGAGERGLEIDRQFVVFTAIGVYLEDLAVSTLGPKWKG 71

Query: 67  KAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEEEE 126
           K A+DL  + DFF  I + P EK  R+ ++K + G QY  ++E        A   Y E E
Sbjct: 72  KTADDLAGNSDFFRDIFTGPFEKFTRITMVKPLSGQQYSEKVEENCVAHWKAAGTYTEAE 131

Query: 127 EAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVV 178
            A +EK  E  +++     + I F    +P    I F    +EGS    E GN V
Sbjct: 132 AAAVEKFKEACKNETFPPGTSILFTHQVSPASLTITF---WREGS--MPETGNTV 181

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BLAST of Carg00166 vs. ExPASy Swiss-Prot
Match: Q6BEH3 (Chalcone--flavanone isomerase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=CHI PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.7e-13
Identity = 65/207 (31.40%), Postives = 101/207 (48.79%), Query Frame = 0


Query: 3   SEVEVVADIAFPNQI---VKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLK- 62
           SEV+V + + FP  I      K   L G G+  LEI    +KFTAIG+YL+   +  L  
Sbjct: 8   SEVQVESYL-FPPTITPPASTKSFLLGGAGVRGLEIGGNFVKFTAIGIYLDETAIPWLAG 67

Query: 63  QWKGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKY 122
           +WKGK AE+L    DFF  I++ P EK  +V +I  + G QY  ++      +  A   Y
Sbjct: 68  KWKGKTAEELADSVDFFADIITGPFEKFTQVTLILPLTGQQYSGKVSENCTAQWKAAGVY 127

Query: 123 EEEEEAQLEKVVEFFRSKYLS-SHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVV 182
              +   +EK ++ F  +  S   S++  H P + TI+    + +  E S   +EN  + 
Sbjct: 128 TPADGKAIEKFLQVFEPQSFSPGDSILFMHSPESLTIS-FGKAGDIPETSNAVIENKKLS 187

Query: 183 EMIKKWYLGGTRGASQTTISSLAKNLA 205
           E + +  + G +G S     SLA  +A
Sbjct: 188 EAVIESVI-GEKGVSPAAKKSLATRIA 211

[Close]

BLAST of Carg00166 vs. ExPASy Swiss-Prot
Match: Q565D8 (Chalcone--flavanone isomerase OS=Gentiana triflora OX=55190 GN=CHI PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.2e-13
Identity = 61/188 (32.45%), Postives = 96/188 (51.06%), Query Frame = 0


Query: 21  KPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLK-QWKGKAAEDLLKDDDFFEAIV 80
           K   L G G+  LEI+   +KFTAIGVYLE + V  L  +WKGK AE+L    +FF  I+
Sbjct: 30  KSFLLGGAGVRGLEINGNFVKFTAIGVYLEESGVAVLSGKWKGKTAEELSDSVEFFTDII 89

Query: 81  SAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEEEEEAQLEKVVEFFRSK-YL 140
           + P EK  +V +I  + G QY  ++      +  A   Y + +   +EK ++ F+++ + 
Sbjct: 90  TGPFEKFTQVTLILPVTGQQYSPKVAENCAAQWKAAGIYTDADGIAIEKFLQVFQTESFT 149

Query: 141 SSHSVISFHFPSAPTIAEIEFSSNE--KEGSKMKVENGNVVEMIKKWYLGGTRGASQTTI 200
              S++  H P + TI+   F  N    E S   +EN  + E + +  + G +G S    
Sbjct: 150 PGDSILFTHSPESLTIS---FGKNGAIPEVSNAVIENKKLSEAVIESII-GEKGVSPAAK 209

Query: 201 SSLAKNLA 205
            SLA  +A
Sbjct: 210 KSLATRIA 213

[Close]

BLAST of Carg00166 vs. ExPASy TrEMBL
Match: A0A6J1FS51 (Chalcone-flavonone isomerase family protein OS=Cucurbita moschata OX=3662 GN=LOC111447946 PE=3 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.7e-106
Identity = 206/209 (98.56%), Postives = 207/209 (99.04%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW
Sbjct: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           KG AAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE
Sbjct: 61  KGNAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEF S+EKEGSKMKVENGNVVEMI
Sbjct: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFLSDEKEGSKMKVENGNVVEMI 180

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRGASQTTISSLAKNLAIELSK
Sbjct: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 209

[Close]

BLAST of Carg00166 vs. ExPASy TrEMBL
Match: A0A6J1JR17 (Chalcone-flavonone isomerase family protein OS=Cucurbita maxima OX=3661 GN=LOC111489046 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 7.4e-102
Identity = 196/209 (93.78%), Postives = 202/209 (96.65%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           MGSEVEVVADIAFP QIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW
Sbjct: 1   MGSEVEVVADIAFPKQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           +GKAAE LLKDDDFFEAIVSAPVEKA+RV VIKEIKGAQY LQLENAVRDRLAADDKYEE
Sbjct: 61  EGKAAEGLLKDDDFFEAIVSAPVEKAVRVAVIKEIKGAQYALQLENAVRDRLAADDKYEE 120

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEK+VEFFRSKYLS+HSVISFHFPSAPTIAEIEFSS+ KEGSKMKVENGNVVEMI
Sbjct: 121 EEEAQLEKIVEFFRSKYLSAHSVISFHFPSAPTIAEIEFSSDGKEGSKMKVENGNVVEMI 180

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRG SQTTISSLA NLAI+LSK
Sbjct: 181 KKWYLGGTRGVSQTTISSLANNLAIQLSK 209

[Close]

BLAST of Carg00166 vs. ExPASy TrEMBL
Match: A0A1S3C525 (Chalcone-flavonone isomerase family protein OS=Cucumis melo OX=3656 GN=LOC103496746 PE=3 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.4e-92
Identity = 176/209 (84.21%), Postives = 196/209 (93.78%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           MGSEVEVVADIAFP QIVK KPLSLLGHG  ++EIHFL+IK TAIGVYLEP++VEHL+QW
Sbjct: 34  MGSEVEVVADIAFPKQIVKAKPLSLLGHGTTEIEIHFLEIKLTAIGVYLEPSVVEHLQQW 93

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           KGKAA+DL++DDDFF+AIVSAPVEK IRVVVIKEIKG+QYG+QLE+AVRDRLAADDKYE+
Sbjct: 94  KGKAAKDLVEDDDFFQAIVSAPVEKVIRVVVIKEIKGSQYGVQLESAVRDRLAADDKYED 153

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEK+VEFF+SKYL +HSVI+FHFP A TIA+IEFSS+EKE SKMKVENGNVVEMI
Sbjct: 154 EEEAQLEKIVEFFQSKYLKTHSVINFHFPPASTIAQIEFSSDEKEESKMKVENGNVVEMI 213

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRG SQ TISSLA NLAIELSK
Sbjct: 214 KKWYLGGTRGVSQPTISSLANNLAIELSK 242

[Close]

BLAST of Carg00166 vs. ExPASy TrEMBL
Match: A0A5D3BCV3 (Chalcone-flavonone isomerase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold233G00110 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.3e-92
Identity = 175/209 (83.73%), Postives = 196/209 (93.78%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           +GSEVEVVADIAFP QIVK KPLSLLGHG  ++EIHFL+IK TAIGVYLEP++VEHL+QW
Sbjct: 8   LGSEVEVVADIAFPKQIVKAKPLSLLGHGTTEIEIHFLEIKLTAIGVYLEPSVVEHLQQW 67

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           KGKAA+DL++DDDFF+AIVSAPVEK IRVVVIKEIKG+QYG+QLE+AVRDRLAADDKYE+
Sbjct: 68  KGKAAKDLVEDDDFFQAIVSAPVEKVIRVVVIKEIKGSQYGVQLESAVRDRLAADDKYED 127

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEK+VEFF+SKYL +HSVI+FHFP A TIA+IEFSS+EKE SKMKVENGNVVEMI
Sbjct: 128 EEEAQLEKIVEFFQSKYLKTHSVINFHFPPASTIAQIEFSSDEKEESKMKVENGNVVEMI 187

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRG SQ TISSLA NLAIELSK
Sbjct: 188 KKWYLGGTRGVSQPTISSLANNLAIELSK 216

[Close]

BLAST of Carg00166 vs. ExPASy TrEMBL
Match: A0A0A0KPA3 (Chalcone-flavonone isomerase family protein OS=Cucumis sativus OX=3659 GN=Csa_5G221960 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.0e-91
Identity = 176/209 (84.21%), Postives = 194/209 (92.82%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVEHLKQW 60
           MGSEVEVVADI FP QIVK KPLSLLGHG  ++EIHFL+IK TAIGVYLEP+IVEHL+QW
Sbjct: 34  MGSEVEVVADIDFPKQIVKAKPLSLLGHGSTEIEIHFLEIKLTAIGVYLEPSIVEHLQQW 93

Query: 61  KGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYEE 120
           KGKAA+DL +DDDFF+AI+SAPVEK IRVVVIKEIKG+QYG+QLE+AVRDRLAADDKYE+
Sbjct: 94  KGKAAKDLEEDDDFFQAIISAPVEKVIRVVVIKEIKGSQYGVQLESAVRDRLAADDKYED 153

Query: 121 EEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEMI 180
           EEEAQLEK+VEFF+SKYL +HSVI+FHFP A TIAEIEFSS+EKE SKMKVENGNVVEMI
Sbjct: 154 EEEAQLEKIVEFFQSKYLKTHSVINFHFPPASTIAEIEFSSDEKEESKMKVENGNVVEMI 213

Query: 181 KKWYLGGTRGASQTTISSLAKNLAIELSK 210
           KKWYLGGTRG SQ TISSLA NLAIELSK
Sbjct: 214 KKWYLGGTRGVSQPTISSLANNLAIELSK 242

[Close]

BLAST of Carg00166 vs. TAIR 10
Match: AT5G05270.1 (Chalcone-flavanone isomerase family protein )

HSP 1 Score: 253.1 bits (645), Expect = 2.0e-67
Identity = 127/209 (60.77%), Postives = 165/209 (78.95%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVE-HLKQ 60
           MG+E+ +V ++ FP QI+ +KPLSLLG GI D+EIHFLQ+KFTAIGVYL+P+ V+ HL  
Sbjct: 1   MGTEMVMVHEVPFPPQIITSKPLSLLGQGITDIEIHFLQVKFTAIGVYLDPSDVKTHLDN 60

Query: 61  WKGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYE 120
           WKGK  ++L  DDDFF+A+ SA +EK IRVVVIKEIKGAQYG+QLEN VRDRLA +DKYE
Sbjct: 61  WKGKTGKELAGDDDFFDALASAEMEKVIRVVVIKEIKGAQYGVQLENTVRDRLAEEDKYE 120

Query: 121 EEEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEM 180
           EEEE +LEKVV FF+SKY  ++SVI++HF +   I EI F +  KE  K+KVEN NVV M
Sbjct: 121 EEEETELEKVVGFFQSKYFKANSVITYHFSAKDGICEIGFETEGKEEEKLKVENANVVGM 180

Query: 181 IKKWYLGGTRGASQTTISSLAKNLAIELS 209
           +++WYL G+RG S +TI S+A +++  L+
Sbjct: 181 MQRWYLSGSRGVSPSTIVSIADSISAVLT 209

[Close]

BLAST of Carg00166 vs. TAIR 10
Match: AT5G05270.2 (Chalcone-flavanone isomerase family protein )

HSP 1 Score: 253.1 bits (645), Expect = 2.0e-67
Identity = 127/209 (60.77%), Postives = 165/209 (78.95%), Query Frame = 0


Query: 1   MGSEVEVVADIAFPNQIVKNKPLSLLGHGIIDLEIHFLQIKFTAIGVYLEPAIVE-HLKQ 60
           MG+E+ +V ++ FP QI+ +KPLSLLG GI D+EIHFLQ+KFTAIGVYL+P+ V+ HL  
Sbjct: 1   MGTEMVMVHEVPFPPQIITSKPLSLLGQGITDIEIHFLQVKFTAIGVYLDPSDVKTHLDN 60

Query: 61  WKGKAAEDLLKDDDFFEAIVSAPVEKAIRVVVIKEIKGAQYGLQLENAVRDRLAADDKYE 120
           WKGK  ++L  DDDFF+A+ SA +EK IRVVVIKEIKGAQYG+QLEN VRDRLA +DKYE
Sbjct: 61  WKGKTGKELAGDDDFFDALASAEMEKVIRVVVIKEIKGAQYGVQLENTVRDRLAEEDKYE 120

Query: 121 EEEEAQLEKVVEFFRSKYLSSHSVISFHFPSAPTIAEIEFSSNEKEGSKMKVENGNVVEM 180
           EEEE +LEKVV FF+SKY  ++SVI++HF +   I EI F +  KE  K+KVEN NVV M
Sbjct: 121 EEEETELEKVVGFFQSKYFKANSVITYHFSAKDGICEIGFETEGKEEEKLKVENANVVGM 180

Query: 181 IKKWYLGGTRGASQTTISSLAKNLAIELS 209
           +++WYL G+RG S +TI S+A +++  L+
Sbjct: 181 MQRWYLSGSRGVSPSTIVSIADSISAVLT 209

The following BLAST results are available for this feature:

BLAST of Carg00166 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7031511.1	4.9e-108	100.00	putative chalcone--flavonone isomerase 3 [Cucurbita argyrosperma subsp. argyrosp...	[more]
KAG6600877.1	1.9e-107	99.52	putative chalcone--flavonone isomerase 3, partial [Cucurbita argyrosperma subsp....	[more]
XP_022943122.1	3.5e-106	98.56	probable chalcone--flavonone isomerase 3 [Cucurbita moschata]	[more]
XP_023527694.1	9.5e-104	96.65	probable chalcone--flavonone isomerase 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022992837.1	1.5e-101	93.78	probable chalcone--flavonone isomerase 3 [Cucurbita maxima]	[more]

BLAST of Carg00166 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A2U7XUE3	5.0e-71	64.11	Chalcone isomerase-like protein 2 OS=Humulus lupulus OX=3486 GN=CHIL2 PE=1 SV=1	[more]
Q8VZW3	2.8e-66	60.77	Probable chalcone--flavanone isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=CHI3...	[more]
Q3Y4F4	1.2e-13	34.86	Chalcone--flavanone isomerase OS=Canna generalis OX=341692 GN=CHI PE=2 SV=1	[more]
Q6BEH3	4.7e-13	31.40	Chalcone--flavanone isomerase OS=Eustoma exaltatum subsp. russellianum OX=52518 ...	[more]
Q565D8	6.2e-13	32.45	Chalcone--flavanone isomerase OS=Gentiana triflora OX=55190 GN=CHI PE=2 SV=1	[more]

BLAST of Carg00166 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FS51	1.7e-106	98.56	Chalcone-flavonone isomerase family protein OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JR17	7.4e-102	93.78	Chalcone-flavonone isomerase family protein OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A1S3C525	2.4e-92	84.21	Chalcone-flavonone isomerase family protein OS=Cucumis melo OX=3656 GN=LOC103496...	[more]
A0A5D3BCV3	5.3e-92	83.73	Chalcone-flavonone isomerase family protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0KPA3	2.0e-91	84.21	Chalcone-flavonone isomerase family protein OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]

BLAST of Carg00166 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05270.1	2.0e-67	60.77	Chalcone-flavanone isomerase family protein	[more]
AT5G05270.2	2.0e-67	60.77	Chalcone-flavanone isomerase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 104..124
IPR016089	Chalcone isomerase, orthogonal bundle domain superfamily	GENE3D	1.10.890.20		coord: 52..204 e-value: 1.2E-77 score: 261.7
IPR016088	Chalcone isomerase, 3-layer sandwich	GENE3D	3.50.70.10		coord: 13..184 e-value: 1.2E-77 score: 261.7
IPR016087	Chalcone isomerase	PFAM	PF02431	Chalcone	coord: 18..203 e-value: 3.0E-28 score: 98.6
IPR044191	Chalcone--flavonone isomerase 3-like	PANTHER	PTHR47588	CHALCONE--FLAVONONE ISOMERASE 3-RELATED	coord: 1..209
IPR036298	Chalcone isomerase superfamily	SUPERFAMILY	54626	Chalcone isomerase	coord: 5..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00166-RA	Carg00166-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009813	flavonoid biosynthetic process
molecular_function	GO:0016872	intramolecular lyase activity