Carg00100 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00100
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1
LocationCarg_Chr04: 5292388 .. 5294918 (+)
RNA-Seq ExpressionCarg00100
SyntenyCarg00100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCAAGGAGCCCTCAACGTGCACGAGAATGGAAGCATTATTTCTTCCCAATCGCCATGGCTGCATCTCATGCTCTCAGCTGAGAAACAAAGACGCAAACCCACACGCAGGAAAATTTTGCGACGGAATGGGGACGTTCATCAAACTTGAGGATTCTCCTATGTTCCTGAAACAGGTAGCCTCCTTTTACGAGTCCCATTGCCGAAATTTAGTTTGTTTGTTCCTGTCGAATCTTGTAGGCTGCTGGATTTGTGGTTAACGATGGATTGATTACAGTTGTGAGCTTGTTTGCTTTTATGCGCGCAGTTGGAATCATTTCTTTATACTTCGAGTCGATTTAATCTTGTTGTTTGTACTTGAACTTGTTTCTTTATTCGTTTGGCATCATTGTCCCTTTCAATGTGTTGGGAGGGTACAACGAGAGAACCTGAGGGTGGTTTAGCCATAGGGAAAGGAGTTTCATTGAAGGTAAACGTCCTTTGTGATTGAAACTATAGCCAAGAAAGGCACACTTGGTGGATCGGAATTCAAGTCTGTGTTCGTTAGAGGGGCGAAGAAAAGGAAAACTTAAGAAATTGAAGACTTTAAAGGGGCTATAACCCACGAGTCAAAATGAACAAGATCAAAAGGTAGCCTATGGATTTTAGCCTTTTGAAAAGAGTCACAAGTGTTAAGATTTATTTCTTTTAGAACCATCAATGTTACAGACTCGCAAAATTTGTCCAACTACAAGTAAAGTATGGCGTCCTGATCTAAATGCCATAAATTGGAGGTGGACTGAGTTAACAAAATAGCGGGTGGCAAGCTTGAATGAAGCGCTCGTGTTGAGGAAGGCCTCGCAATTAGCTTATATAGACCACCATCAAGATGATCCTGAAGGAGAATCTTCTTGGACAATAGAAGGGTTAAGATTCAATAGAGGTCATATTGTCTCAACATAATTTGGAAAGGCCAATGAGTCTATGCAAGATAGGAGGTTCACATAAGACTTGAGAAATTAATAGGAAATGAAGAAGGTGATAGTAAAGATGCATAACTAGCATGCGAGATAGGGATAAAAGTACCATTACCGACAAGCCCCATTATGCGACATAGATGGTGCCAGAGTTTATACACCATTGCATCGTGGAGCATGGCACTGGATCGGGTAAGGTTGGGGGATTGGACTGGATAAAATGGCGTGGGTTGTGTGAATTTGGGCTGCTAGAGCTTGGATTAAATTGAGTAAAAAAGGATTGAGGTTGTTGGGTATCGTATGGTTGGTAACTCAAATTTGCACTATGCCCAAAATTTTCACATATTTGGTACTGTGTTTTACCTCCTTTAGCTGCTGTATTCAAGGTATTTTTGGGCCAACGCCCTTGATTGGAAGCTGATTGAGCAAGAATTAATGAGCTGTGGGTGGATTTTAGTGAGGTTTTGCTGTCGTTGTGGGGGTTGAGGAAGAAGACCCATGTCCATTTCCAGATGAGACAGGTGGGTTTGGTGGTTGTGGCATTTCTGAAGCAAGAAGAAAGATTTCTAGGTCAGCTCTAATACAATGATACTGTATAATGCTTAGAAGGAAAGGGAGAGAATCGAGGAGAGAGAAGTTGTAAAAATTGTATTCACCAAAAGAAAAGAACGGTGCAGAAAATATGATAAAGTTTCCTAAAACATGTTACAGATGAAGGGGGATAATAATATAAAAGAGAATAACACAAAGGCTAATAACAGAATTTATTTTGCTTTTATTATTTTTTAATAAGAAACTGAGCTCTCATTAAGGAAAAAAATGAAATAATACTAGAGCATACAAAAAAGAGAACTCACAAAACAAGGAGAGTAATAACTAAATAATAATCCTTAAACTGATGGTTTAAGAGTCCCCCCGCAAAAAAAAAAAAAAAGTTTATTAAATAGCTTTTAAGTGGAATTATGGAGCCTGAGGAGTAAATAGAGAGAAAAAAGAGAAAAAAGAAAAAAAAGAAAAGAAACATTCGTGGTTCTTCTTTTGCTTGTCACTGGAAATCATGTGTTTAATACTTTACTGTTCTTGTTTTTGGAGTTTATAGACTTGTTTATCATCTTCTATGGTACATTGTGAGTGGAAGCCATCTATTGGAAGTTGATTCAGTTGGCAGCGATTAATGTTAAAAATCTTCCGGAACTTATATTACTGTGTGAGTTAACTTGTTGTACTGTCCCAGCTCTGGATGGGAACATTCGATTTGCATGGTTCGAAATAAGCATAAAGATACTTAGTTTCTCTATCAATTCACGTTCTCAATCTTTTTCCTATTTAACATGCAATATGAATTCTAGAATATTAGCTTTTCCTCAAATCACTTGTAATAAATTGTCTTTGGTTGTCTTTGGAAGCTCTTCTTCTAAAACTTAGATTTTTTTCTTTATTAGGCAATCAGATGGCTTTCCTTACCTTTTATTTTGTCTTCCCATTATTTATAGATATTTTCTCTAGAACAACCAACTGATGAGTTGGAAGAACGATGCAGAAGACTACACGATGGATGTAGGATATTTATGTAA

mRNA sequence

ATGACCAAGGAGCCCTCAACGTGCACGAGAATGGAAGCATTATTTCTTCCCAATCGCCATGGCTGCATCTCATGCTCTCAGCTGAGAAACAAAGACGCAAACCCACACGCAGGAAAATTTTGCGACGGAATGGGGACGTTCATCAAACTTGAGGATTCTCCTATGTTCCTGAAACAGATATTTTCTCTAGAACAACCAACTGATGAGTTGGAAGAACGATGCAGAAGACTACACGATGGATGTAGGATATTTATGTAA

Coding sequence (CDS)

ATGACCAAGGAGCCCTCAACGTGCACGAGAATGGAAGCATTATTTCTTCCCAATCGCCATGGCTGCATCTCATGCTCTCAGCTGAGAAACAAAGACGCAAACCCACACGCAGGAAAATTTTGCGACGGAATGGGGACGTTCATCAAACTTGAGGATTCTCCTATGTTCCTGAAACAGATATTTTCTCTAGAACAACCAACTGATGAGTTGGAAGAACGATGCAGAAGACTACACGATGGATGTAGGATATTTATGTAA

Protein sequence

MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCRIFM
Homology
[Close]
BLAST of Carg00100 vs. NCBI nr
Match: XP_022941999.1 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X9 [Cucurbita moschata])

HSP 1 Score: 186.8 bits (473), Expect = 7.7e-44
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 0 

Query: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60
          MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI
Sbjct: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60

Query: 61 FSLEQPTDELEERCRRLHDGCRIFM 86
          FSLEQPTDELEERCRRLHDGCRIFM
Sbjct: 61 FSLEQPTDELEERCRRLHDGCRIFM 85

[Close]
BLAST of Carg00100 vs. NCBI nr
Match: KAG7031446.1 (ADP-ribosylation factor GTPase-activating protein AGD2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 186.8 bits (473), Expect = 7.7e-44
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 0 

Query: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60
          MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI
Sbjct: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60

Query: 61 FSLEQPTDELEERCRRLHDGCRIFM 86
          FSLEQPTDELEERCRRLHDGCRIFM
Sbjct: 61 FSLEQPTDELEERCRRLHDGCRIFM 85

[Close]
BLAST of Carg00100 vs. NCBI nr
Match: XP_022941987.1 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 186.8 bits (473), Expect = 7.7e-44
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 0 

Query: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60
          MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI
Sbjct: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60

Query: 61 FSLEQPTDELEERCRRLHDGCRIFM 86
          FSLEQPTDELEERCRRLHDGCRIFM
Sbjct: 61 FSLEQPTDELEERCRRLHDGCRIFM 85

[Close]
BLAST of Carg00100 vs. NCBI nr
Match: KAG6600810.1 (ADP-ribosylation factor GTPase-activating protein AGD4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 186.8 bits (473), Expect = 7.7e-44
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 0 

Query: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60
          MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI
Sbjct: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60

Query: 61 FSLEQPTDELEERCRRLHDGCRIFM 86
          FSLEQPTDELEERCRRLHDGCRIFM
Sbjct: 61 FSLEQPTDELEERCRRLHDGCRIFM 85

[Close]
BLAST of Carg00100 vs. NCBI nr
Match: XP_022986926.1 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 90.9 bits (224), Expect = 5.7e-15
Identity = 40/42 (95.24%), Postives = 42/42 (100.00%), Query Frame = 0 

Query: 44 MGTFIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCRIFM 86
          MGTFIKLEDSPMFLKQ+FSLEQPTDELE+RCRRLHDGCRIFM
Sbjct: 1  MGTFIKLEDSPMFLKQVFSLEQPTDELEDRCRRLHDGCRIFM 42

[Close]
BLAST of Carg00100 vs. ExPASy Swiss-Prot
Match: Q9C6C3 (ADP-ribosylation factor GTPase-activating protein AGD2 OS=Arabidopsis thaliana OX=3702 GN=AGD2 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 9.2e-08
Identity = 25/42 (59.52%), Postives = 33/42 (78.57%), Query Frame = 0 

Query: 44 MGTFIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCRIFM 86
          M  FI LEDSPMF KQ+FSLE  +DEL++RC++L+ G + FM
Sbjct: 1  MAGFINLEDSPMFQKQVFSLEGTSDELKDRCQKLYKGVKKFM 42

[Close]
BLAST of Carg00100 vs. ExPASy Swiss-Prot
Match: Q9SMX5 (ADP-ribosylation factor GTPase-activating protein AGD4 OS=Arabidopsis thaliana OX=3702 GN=AGD4 PE=2 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 1.6e-07
Identity = 25/42 (59.52%), Postives = 32/42 (76.19%), Query Frame = 0 

Query: 44 MGTFIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCRIFM 86
          M TFI LEDSPMF KQ+ SLE   DEL++RC++L+ G + FM
Sbjct: 1  MATFINLEDSPMFQKQVCSLEGTADELKDRCQKLYKGVKKFM 42

[Close]
BLAST of Carg00100 vs. ExPASy TrEMBL
Match: A0A6J1FQ24 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X9 OS=Cucurbita moschata OX=3662 GN=LOC111447192 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.7e-44
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 0 

Query: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60
          MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI
Sbjct: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60

Query: 61 FSLEQPTDELEERCRRLHDGCRIFM 86
          FSLEQPTDELEERCRRLHDGCRIFM
Sbjct: 61 FSLEQPTDELEERCRRLHDGCRIFM 85

[Close]
BLAST of Carg00100 vs. ExPASy TrEMBL
Match: A0A6J1FP00 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447192 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.7e-44
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 0 

Query: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60
          MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI
Sbjct: 1  MTKEPSTCTRMEALFLPNRHGCISCSQLRNKDANPHAGKFCDGMGTFIKLEDSPMFLKQI 60

Query: 61 FSLEQPTDELEERCRRLHDGCRIFM 86
          FSLEQPTDELEERCRRLHDGCRIFM
Sbjct: 61 FSLEQPTDELEERCRRLHDGCRIFM 85

[Close]
BLAST of Carg00100 vs. ExPASy TrEMBL
Match: A0A6J1JCM9 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484517 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.8e-15
Identity = 40/42 (95.24%), Postives = 42/42 (100.00%), Query Frame = 0 

Query: 44 MGTFIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCRIFM 86
          MGTFIKLEDSPMFLKQ+FSLEQPTDELE+RCRRLHDGCRIFM
Sbjct: 1  MGTFIKLEDSPMFLKQVFSLEQPTDELEDRCRRLHDGCRIFM 42

[Close]
BLAST of Carg00100 vs. ExPASy TrEMBL
Match: A0A1S3C009 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495062 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.7e-12
Identity = 36/42 (85.71%), Postives = 40/42 (95.24%), Query Frame = 0 

Query: 44 MGTFIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCRIFM 86
          MG FIKLEDSPMFLKQIFSLE+ TDELE+RCRRLH+GCR+FM
Sbjct: 1  MGAFIKLEDSPMFLKQIFSLERTTDELEDRCRRLHNGCRMFM 42

[Close]
BLAST of Carg00100 vs. ExPASy TrEMBL
Match: A0A1S3C0G9 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495062 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.7e-12
Identity = 36/42 (85.71%), Postives = 40/42 (95.24%), Query Frame = 0 

Query: 44 MGTFIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCRIFM 86
          MG FIKLEDSPMFLKQIFSLE+ TDELE+RCRRLH+GCR+FM
Sbjct: 1  MGAFIKLEDSPMFLKQIFSLERTTDELEDRCRRLHNGCRMFM 42

[Close]
BLAST of Carg00100 vs. TAIR 10
Match: AT1G60860.1 (ARF-GAP domain 2 )

HSP 1 Score: 57.4 bits (137), Expect = 6.6e-09
Identity = 25/42 (59.52%), Postives = 33/42 (78.57%), Query Frame = 0 

Query: 44 MGTFIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCRIFM 86
          M  FI LEDSPMF KQ+FSLE  +DEL++RC++L+ G + FM
Sbjct: 1  MAGFINLEDSPMFQKQVFSLEGTSDELKDRCQKLYKGVKKFM 42

[Close]
BLAST of Carg00100 vs. TAIR 10
Match: AT1G10870.1 (ARF-GAP domain 4 )

HSP 1 Score: 56.6 bits (135), Expect = 1.1e-08
Identity = 25/42 (59.52%), Postives = 32/42 (76.19%), Query Frame = 0 

Query: 44 MGTFIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCRIFM 86
          M TFI LEDSPMF KQ+ SLE   DEL++RC++L+ G + FM
Sbjct: 1  MATFINLEDSPMFQKQVCSLEGTADELKDRCQKLYKGVKKFM 42

[Close]
BLAST of Carg00100 vs. TAIR 10
Match: AT5G61980.1 (ARF-GAP domain 1 )

HSP 1 Score: 42.4 bits (98), Expect = 2.2e-04
Identity = 18/36 (50.00%), Postives = 25/36 (69.44%), Query Frame = 0 

Query: 47 FIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCR 83
          F KL+DSPMF +Q+ S+E+  + L  RC R + GCR
Sbjct: 3  FAKLDDSPMFRQQMQSMEESAELLRLRCLRFYKGCR 38

[Close]
BLAST of Carg00100 vs. TAIR 10
Match: AT5G13300.1 (ARF GTPase-activating protein )

HSP 1 Score: 42.4 bits (98), Expect = 2.2e-04
Identity = 18/36 (50.00%), Postives = 25/36 (69.44%), Query Frame = 0 

Query: 47 FIKLEDSPMFLKQIFSLEQPTDELEERCRRLHDGCR 83
          F KL+DSPMF KQ+ S+E+  + L ER  + + GCR
Sbjct: 3  FTKLDDSPMFRKQLQSMEESAEILRERSLKFYKGCR 38

The following BLAST results are available for this feature:
BLAST of Carg00100 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022941999.17.7e-44100.00ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X9 [Cucurbit... [more]
KAG7031446.17.7e-44100.00ADP-ribosylation factor GTPase-activating protein AGD2, partial [Cucurbita argyr... [more]
XP_022941987.17.7e-44100.00ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 [Cucurbit... [more]
KAG6600810.17.7e-44100.00ADP-ribosylation factor GTPase-activating protein AGD4, partial [Cucurbita argyr... [more]
XP_022986926.15.7e-1595.24ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 [Cucurbit... [more]
BLAST of Carg00100 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9C6C39.2e-0859.52ADP-ribosylation factor GTPase-activating protein AGD2 OS=Arabidopsis thaliana O... [more]
Q9SMX51.6e-0759.52ADP-ribosylation factor GTPase-activating protein AGD4 OS=Arabidopsis thaliana O... [more]
BLAST of Carg00100 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FQ243.7e-44100.00ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X9 OS=Cucurb... [more]
A0A6J1FP003.7e-44100.00ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 OS=Cucurb... [more]
A0A6J1JCM92.8e-1595.24ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 OS=Cucurb... [more]
A0A1S3C0091.7e-1285.71ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 OS=Cucumi... [more]
A0A1S3C0G91.7e-1285.71ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X2 OS=Cucumi... [more]
BLAST of Carg00100 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G60860.16.6e-0959.52ARF-GAP domain 2 [more]
AT1G10870.11.1e-0859.52ARF-GAP domain 4 [more]
AT5G61980.12.2e-0450.00ARF-GAP domain 1 [more]
AT5G13300.12.2e-0450.00ARF GTPase-activating protein [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00100-RACarg00100-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016020 membrane
molecular_function GO:0005096 GTPase activator activity
molecular_function GO:0046872 metal ion binding