Carg00070 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00070
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionnuclear intron maturase 3, mitochondrial
LocationCarg_Chr04: 5133453 .. 5135759 (-)
RNA-Seq ExpressionCarg00070
SyntenyCarg00070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCATCCACCTAAGAAAGATAGCGACACCATTGAAACCCAAGCTCTCATCTTCCTTCAACAAACTTTACTCTGATAGCCCATTGAAGCATTCGCCTCTTCTCAAGCTTCCGTTTCAACATTCTGTACAAACCCTCACAAGGCCCCAACTCGAGGCTCTAGTTCTATCCCGATTCTCCCAGGGCAAGTTCTTCGACCTTCTTCAAAATGTTGTCGCCTCTCCCTCTGTTCTTTTCACCGCCTCCCAAAATCTCATCACTCCACTCCCAAGCAATCGCCTCAATGCTCCCGAGTCGCTACTCAGTCTCGATATGGTCTCTAGTTGCTTTTCGGTCGAGGACATGGCTCGGGAGCTCTACGAGAATCGTTTCGATGTTGGTGCTTGCTGTGTTCGGTTGGAACCATCGGAAGAGAAAGGTGAGTTTCTGGTCTTACCGAATTTGAAATTGAAGGTCTTACTCGAGGCTATTAGGATGGTGTTGGAAATTGTTTATGACGAACGATTTGTAACGTTCTCTTATGGGGGGCGTGTCGGTATGGGGCGGCACACTGCCATTAGGTACCTGAAGAACTCGGTACAAAATCCTAGTTGGTGGTTTACCGTTGCATTTCGTCGCAGAAAGTTCGATTCTGTACATGTAAATAAGTTGTGCTTATTGGTGCAAGAGAAAATTAAGGATGATATTTTGATTCTTATGCTAAAGAAACTGTTTGAATTGGAGGCAGTTCAAATTGAATTGGGTGGTTGTTATTTAGGAAGGGGGTTCCCTCAGGAAAGTGGTTTGTGTTCAATCTTGTCTAATATATACTTCAATGGCTTTGATAAAGAAATTCAACAAATACGTCTTCAAAAGAATGAAGAAAATCCCAAGTTCAGTCTGGACGGTACTGTTTCTTTCCATAATCCGGTGAAAATATATGCCGTTAGGTATCTGGATGAGATATTAGTTATAACATCGGGGTCGAAGATGCTAATAATGGAGTTGAAAAGCCAGGTGCTAAGGTATTTAGAAGGGAATTTAGAATTGGAAGTGGATCGAATGAATACTGCAATTCATAGTGCTGTCTCCGAGAAAATTAGTTTCTTAGGAATGGAACTACAGGCAGTACCACCTTCAGTTCTGCATCCACCAATGTCCGAAAAGGCAATCAGGGCTCGGAAGAAGTACCTTAGACAGAAGGAAGTTAGAGCAATAGAATTGAGAAATGCCCGTGAGAGAAACAGGAAAAAATTGGGATTGAAGATATTGAGTCACGTGTTCAAGAAATTGAAGCGAACCGATGGCTTGAAATCTGAATTCCAAATCGAGAAGGAAGTTACAGAAATCTTCAGAAATTGGGCCGATGAAGTAGTGCGAGATTTCTTTGAGTCTTTGGAAGATAATACAGAGTGGCACCGTCCGCTGTCAGCAGGTGATTTCCTCTCCTTAAAACACATAAGAAATCAGTTGCCAGTAGATCTTGTGAATGCTTATGACAGGTTTCAAGATCAGGTAAACCAGCACTTGAATCCTCTAAAGGCCAAAGAGGAGAAGGCTAGGGAGGATGAAGAGAAAAGATTGGGTGAAGAAGAACGATATGCTAAAAGAACAGTTGAGGACTTAACAAGGCTATGCATCAAAGTTGAAGCTCCTATAGAGCTTGTTAGGAAGGCAGTGAAGATGATTGGATTTACAAATAAAATGGGCCGTCCTCGGCCTATCAGTTCTCTCATTGCTCTTGAAGATACAGATATTATCAAGTGGTATGCCGGTGTAGGAAGAAGGTGGTTAGACTTCTTTTGCTGTTGTCATAACTATAAGATGGTCAAAACTATTGTAACTTACCATTTGAGGTTTTCTTGTATTTTGACATTGGCGGAAAAGCATGAATCGACCAAACGGGAAGCCATGAAACATTACAGTAAAGATTTGAAAGTCTTTGATTTAAATGGCAAGGAAGAGATGCACTTCCCAACAGAAAGAGAAGTTAAGATGTTGGGAGAAAGAAATCTTGCTGACCCATACCCTGTGGATGGGGCTTTTTCTTTGTTTCTGATTAGATTAGTCACTGATGAAGATTCATATCCTTGTATTGCTCATTTTTGCAATAGAACAGACTCTATTTTATATAGGGTCCGATTACTGCAAAAGACTCTGAATGTCAATCCATCTAATGGAGTGGAATGGGTGAGAGGGATGGGAGTGATTCATGAAAGTTTAAATCAGAGATGCCTCCCTCTATGTGCTGATCACATCAATGATTTATACATGGGGAAAATCAACCTTCAAGACTTGGACTGCACCTTATCGTTGGATATGGACTGA

mRNA sequence

ATGCTCATCCACCTAAGAAAGATAGCGACACCATTGAAACCCAAGCTCTCATCTTCCTTCAACAAACTTTACTCTGATAGCCCATTGAAGCATTCGCCTCTTCTCAAGCTTCCGTTTCAACATTCTGTACAAACCCTCACAAGGCCCCAACTCGAGGCTCTAGTTCTATCCCGATTCTCCCAGGGCAAGTTCTTCGACCTTCTTCAAAATGTTGTCGCCTCTCCCTCTGTTCTTTTCACCGCCTCCCAAAATCTCATCACTCCACTCCCAAGCAATCGCCTCAATGCTCCCGAGTCGCTACTCAGTCTCGATATGGTCTCTAGTTGCTTTTCGGTCGAGGACATGGCTCGGGAGCTCTACGAGAATCGTTTCGATGTTGGTGCTTGCTGTGTTCGGTTGGAACCATCGGAAGAGAAAGGTGAGTTTCTGGTCTTACCGAATTTGAAATTGAAGGTCTTACTCGAGGCTATTAGGATGGTGTTGGAAATTGTTTATGACGAACGATTTGTAACGTTCTCTTATGGGGGGCGTGTCGGTATGGGGCGGCACACTGCCATTAGGTACCTGAAGAACTCGGTACAAAATCCTAGTTGGTGGTTTACCGTTGCATTTCGTCGCAGAAAGTTCGATTCTGTACATGTAAATAAGTTGTGCTTATTGGTGCAAGAGAAAATTAAGGATGATATTTTGATTCTTATGCTAAAGAAACTGTTTGAATTGGAGGCAGTTCAAATTGAATTGGGTGGTTGTTATTTAGGAAGGGGGTTCCCTCAGGAAAGTGGTTTGTGTTCAATCTTGTCTAATATATACTTCAATGGCTTTGATAAAGAAATTCAACAAATACGTCTTCAAAAGAATGAAGAAAATCCCAAGTTCAGTCTGGACGGTACTGTTTCTTTCCATAATCCGGTGAAAATATATGCCGTTAGGTATCTGGATGAGATATTAGTTATAACATCGGGGTCGAAGATGCTAATAATGGAGTTGAAAAGCCAGGTGCTAAGGTATTTAGAAGGGAATTTAGAATTGGAAGTGGATCGAATGAATACTGCAATTCATAGTGCTGTCTCCGAGAAAATTAGTTTCTTAGGAATGGAACTACAGGCAGTACCACCTTCAGTTCTGCATCCACCAATGTCCGAAAAGGCAATCAGGGCTCGGAAGAAGTACCTTAGACAGAAGGAAGTTAGAGCAATAGAATTGAGAAATGCCCGTGAGAGAAACAGGAAAAAATTGGGATTGAAGATATTGAGTCACGTGTTCAAGAAATTGAAGCGAACCGATGGCTTGAAATCTGAATTCCAAATCGAGAAGGAAGTTACAGAAATCTTCAGAAATTGGGCCGATGAAGTAGTGCGAGATTTCTTTGAGTCTTTGGAAGATAATACAGAGTGGCACCGTCCGCTGTCAGCAGGTGATTTCCTCTCCTTAAAACACATAAGAAATCAGTTGCCAGTAGATCTTGTGAATGCTTATGACAGGTTTCAAGATCAGGTAAACCAGCACTTGAATCCTCTAAAGGCCAAAGAGGAGAAGGCTAGGGAGGATGAAGAGAAAAGATTGGGTGAAGAAGAACGATATGCTAAAAGAACAGTTGAGGACTTAACAAGGCTATGCATCAAAGTTGAAGCTCCTATAGAGCTTGTTAGGAAGGCAGTGAAGATGATTGGATTTACAAATAAAATGGGCCGTCCTCGGCCTATCAGTTCTCTCATTGCTCTTGAAGATACAGATATTATCAAGTGGTATGCCGGTGTAGGAAGAAGGTGGTTAGACTTCTTTTGCTGTTGTCATAACTATAAGATGGTCAAAACTATTGTAACTTACCATTTGAGGTTTTCTTGTATTTTGACATTGGCGGAAAAGCATGAATCGACCAAACGGGAAGCCATGAAACATTACAGTAAAGATTTGAAAGTCTTTGATTTAAATGGCAAGGAAGAGATGCACTTCCCAACAGAAAGAGAAGTTAAGATGTTGGGAGAAAGAAATCTTGCTGACCCATACCCTGTGGATGGGGCTTTTTCTTTGTTTCTGATTAGATTAGTCACTGATGAAGATTCATATCCTTGTATTGCTCATTTTTGCAATAGAACAGACTCTATTTTATATAGGGTCCGATTACTGCAAAAGACTCTGAATGTCAATCCATCTAATGGAGTGGAATGGGTGAGAGGGATGGGAGTGATTCATGAAAGTTTAAATCAGAGATGCCTCCCTCTATGTGCTGATCACATCAATGATTTATACATGGGGAAAATCAACCTTCAAGACTTGGACTGCACCTTATCGTTGGATATGGACTGA

Coding sequence (CDS)

ATGCTCATCCACCTAAGAAAGATAGCGACACCATTGAAACCCAAGCTCTCATCTTCCTTCAACAAACTTTACTCTGATAGCCCATTGAAGCATTCGCCTCTTCTCAAGCTTCCGTTTCAACATTCTGTACAAACCCTCACAAGGCCCCAACTCGAGGCTCTAGTTCTATCCCGATTCTCCCAGGGCAAGTTCTTCGACCTTCTTCAAAATGTTGTCGCCTCTCCCTCTGTTCTTTTCACCGCCTCCCAAAATCTCATCACTCCACTCCCAAGCAATCGCCTCAATGCTCCCGAGTCGCTACTCAGTCTCGATATGGTCTCTAGTTGCTTTTCGGTCGAGGACATGGCTCGGGAGCTCTACGAGAATCGTTTCGATGTTGGTGCTTGCTGTGTTCGGTTGGAACCATCGGAAGAGAAAGGTGAGTTTCTGGTCTTACCGAATTTGAAATTGAAGGTCTTACTCGAGGCTATTAGGATGGTGTTGGAAATTGTTTATGACGAACGATTTGTAACGTTCTCTTATGGGGGGCGTGTCGGTATGGGGCGGCACACTGCCATTAGGTACCTGAAGAACTCGGTACAAAATCCTAGTTGGTGGTTTACCGTTGCATTTCGTCGCAGAAAGTTCGATTCTGTACATGTAAATAAGTTGTGCTTATTGGTGCAAGAGAAAATTAAGGATGATATTTTGATTCTTATGCTAAAGAAACTGTTTGAATTGGAGGCAGTTCAAATTGAATTGGGTGGTTGTTATTTAGGAAGGGGGTTCCCTCAGGAAAGTGGTTTGTGTTCAATCTTGTCTAATATATACTTCAATGGCTTTGATAAAGAAATTCAACAAATACGTCTTCAAAAGAATGAAGAAAATCCCAAGTTCAGTCTGGACGGTACTGTTTCTTTCCATAATCCGGTGAAAATATATGCCGTTAGGTATCTGGATGAGATATTAGTTATAACATCGGGGTCGAAGATGCTAATAATGGAGTTGAAAAGCCAGGTGCTAAGGTATTTAGAAGGGAATTTAGAATTGGAAGTGGATCGAATGAATACTGCAATTCATAGTGCTGTCTCCGAGAAAATTAGTTTCTTAGGAATGGAACTACAGGCAGTACCACCTTCAGTTCTGCATCCACCAATGTCCGAAAAGGCAATCAGGGCTCGGAAGAAGTACCTTAGACAGAAGGAAGTTAGAGCAATAGAATTGAGAAATGCCCGTGAGAGAAACAGGAAAAAATTGGGATTGAAGATATTGAGTCACGTGTTCAAGAAATTGAAGCGAACCGATGGCTTGAAATCTGAATTCCAAATCGAGAAGGAAGTTACAGAAATCTTCAGAAATTGGGCCGATGAAGTAGTGCGAGATTTCTTTGAGTCTTTGGAAGATAATACAGAGTGGCACCGTCCGCTGTCAGCAGGTGATTTCCTCTCCTTAAAACACATAAGAAATCAGTTGCCAGTAGATCTTGTGAATGCTTATGACAGGTTTCAAGATCAGGTAAACCAGCACTTGAATCCTCTAAAGGCCAAAGAGGAGAAGGCTAGGGAGGATGAAGAGAAAAGATTGGGTGAAGAAGAACGATATGCTAAAAGAACAGTTGAGGACTTAACAAGGCTATGCATCAAAGTTGAAGCTCCTATAGAGCTTGTTAGGAAGGCAGTGAAGATGATTGGATTTACAAATAAAATGGGCCGTCCTCGGCCTATCAGTTCTCTCATTGCTCTTGAAGATACAGATATTATCAAGTGGTATGCCGGTGTAGGAAGAAGGTGGTTAGACTTCTTTTGCTGTTGTCATAACTATAAGATGGTCAAAACTATTGTAACTTACCATTTGAGGTTTTCTTGTATTTTGACATTGGCGGAAAAGCATGAATCGACCAAACGGGAAGCCATGAAACATTACAGTAAAGATTTGAAAGTCTTTGATTTAAATGGCAAGGAAGAGATGCACTTCCCAACAGAAAGAGAAGTTAAGATGTTGGGAGAAAGAAATCTTGCTGACCCATACCCTGTGGATGGGGCTTTTTCTTTGTTTCTGATTAGATTAGTCACTGATGAAGATTCATATCCTTGTATTGCTCATTTTTGCAATAGAACAGACTCTATTTTATATAGGGTCCGATTACTGCAAAAGACTCTGAATGTCAATCCATCTAATGGAGTGGAATGGGTGAGAGGGATGGGAGTGATTCATGAAAGTTTAAATCAGAGATGCCTCCCTCTATGTGCTGATCACATCAATGATTTATACATGGGGAAAATCAACCTTCAAGACTTGGACTGCACCTTATCGTTGGATATGGACTGA

Protein sequence

MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFSQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELYENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSFHNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHIRNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCIKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKMLGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNGVEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD
Homology
BLAST of Carg00070 vs. NCBI nr
Match: KAG7031416.1 (Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1519.6 bits (3933), Expect = 0.0e+00
Identity = 768/768 (100.00%), Postives = 768/768 (100.00%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60
           MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120
           QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120

Query: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180
           ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300
           EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480
           FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540
           RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660
           KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720
           GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 768

BLAST of Carg00070 vs. NCBI nr
Match: KAG6600779.1 (Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1516.9 bits (3926), Expect = 0.0e+00
Identity = 766/768 (99.74%), Postives = 768/768 (100.00%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60
           MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120
           QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120

Query: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180
           ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300
           EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFS+DGTVSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSMDGTVSF 300

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480
           FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540
           RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660
           KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720
           GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHI+DLYMGKINLQDLDCTLSLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Carg00070 vs. NCBI nr
Match: XP_022942578.1 (nuclear intron maturase 3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 760/768 (98.96%), Postives = 764/768 (99.48%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60
           MLIHLRKIATPLKPKLSSSFNKLYSD PLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSFNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120
           QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAP+SLLSLDMVSSCFSVEDMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSLDMVSSCFSVEDMARELY 120

Query: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180
           ENRFDVGACCVRLE SEEKGEFLVLPNLKLKVLLEAIR+VLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300
           EAVQIELGGCYLGRG PQESGLCSILSNIYFNGFDKEIQQIRL+KNEENPKFSLDGTVSF
Sbjct: 241 EAVQIELGGCYLGRGLPQESGLCSILSNIYFNGFDKEIQQIRLEKNEENPKFSLDGTVSF 300

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAV PSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVLPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480
           FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540
           RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660
           KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720
           GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHI+DLYMGKINLQDLDCTLSLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Carg00070 vs. NCBI nr
Match: XP_022976163.1 (nuclear intron maturase 3, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1495.7 bits (3871), Expect = 0.0e+00
Identity = 756/768 (98.44%), Postives = 763/768 (99.35%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60
           MLIHLRKIATPLKPKLSSS NKLYSD PLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSLNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120
           QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120

Query: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180
           ENRFDVGACCVRLE SEEKGEFLVLPNLKLKVLLEA++MVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAMKMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300
           EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSL+GTVSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLNGTVSF 300

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           HNPVKIYAVRYLDEILVITSGSKM IMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMQIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480
           FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540
           RNQLPVDLVNAYDRFQDQVN+HLNPLKAK+EKAREDEEKRL EEERYAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNKHLNPLKAKKEKAREDEEKRLVEEERYAKRTVEDLTRLCI 540

Query: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KVEAPIELVRKAVKMIGFTNKMGRP+PISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPQPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660
           KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720
           GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHI+DLYMGKINLQDLDCTLSLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Carg00070 vs. NCBI nr
Match: XP_023544815.1 (nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544823.1 nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544830.1 nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544839.1 nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1489.2 bits (3854), Expect = 0.0e+00
Identity = 751/768 (97.79%), Postives = 759/768 (98.83%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60
           MLIHLRKIATP KP LSSSFNKLYSD PLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS
Sbjct: 1   MLIHLRKIATPWKPNLSSSFNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120
           QGKFFDLL+NVVASPSVLFTASQNLITPLPSNRLNAP+SLLS DMVSSCFSVEDMARELY
Sbjct: 61  QGKFFDLLRNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSFDMVSSCFSVEDMARELY 120

Query: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180
           ENRFDVGACCVRLE SEEKGEFLVLPNLKLKVLLEAIR+VLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300
           EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKE+QQIRL+KNEENPKFSLDGTVSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKELQQIRLEKNEENPKFSLDGTVSF 300

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKIL HV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILGHV 420

Query: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480
           FKKLKRTDGLK EFQIEKEVTEIFRNWADEVVRDF ESLEDNTEWHRPLSAGDFLSLKHI
Sbjct: 421 FKKLKRTDGLKYEFQIEKEVTEIFRNWADEVVRDFLESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540
           RNQLPVDLVNAYDRFQDQVNQHLNPLKAK+EKAREDE+KRLGEEERYAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKKEKAREDEKKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660
           K VKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML
Sbjct: 601 KTVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720
           GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHI+DLYMGKINLQDLDCTLSLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Carg00070 vs. ExPASy Swiss-Prot
Match: Q9LZA5 (Nuclear intron maturase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT3 PE=3 SV=2)

HSP 1 Score: 807.7 bits (2085), Expect = 1.1e-232
Identity = 436/769 (56.70%), Postives = 550/769 (71.52%), Query Frame = 0

Query: 12  LKPKLSSSFNKLYS---DSPLKHSPLLKLPFQHSVQTLTRP----QLEALVLSRFSQGKF 71
           L+ ++ S +N+  S    S L++       F +S QT+T P    +LEALVL ++S GKF
Sbjct: 3   LRLRVHSFYNRGISFLVSSSLRNLSTASSLFLNSDQTITEPLVKSELEALVLKQYSHGKF 62

Query: 72  FDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELYENRF 131
           + L++N V+ P VL  A QNL        L+A  S    D VS  FS+E+M RE+ E RF
Sbjct: 63  YSLVKNAVSLPCVLLAACQNL-------SLSANSSGDLADRVSRRFSIEEMGREIREGRF 122

Query: 132 DVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGMGRHT 191
           D+ +CCV    S      LVLPNLKLKVL+EAIRMVLEIVYD+RF TFSYGGRVGMGRHT
Sbjct: 123 DIRSCCVEFISSS-----LVLPNLKLKVLIEAIRMVLEIVYDDRFATFSYGGRVGMGRHT 182

Query: 192 AIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQ 251
           AIRYLKNSV+NP WWF V+F R  F+  +V+ LC  V EKI D +LI M+KKLFE   ++
Sbjct: 183 AIRYLKNSVENPRWWFRVSFAREMFEERNVDILCGFVGEKINDVMLIEMIKKLFEFGILK 242

Query: 252 IELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVS----F 311
           IELGGC  GRGFPQE GLCSIL N+YF+G DKEIQ +RL+   +NP+       S    F
Sbjct: 243 IELGGCNSGRGFPQECGLCSILINVYFDGLDKEIQDLRLKMKVKNPRVGTGDEESTGNVF 302

Query: 312 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 371
             PV IYAVRYLDEILVITSGSKML M+LK +++  LE  LEL VDR+NT+IHSAVSEKI
Sbjct: 303 FKPVNIYAVRYLDEILVITSGSKMLTMDLKKRIVDILEQRLELRVDRLNTSIHSAVSEKI 362

Query: 372 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 431
           +FLGM LQAVPPSVL PP SEKA+RA KKY RQK+VR +ELRNARERNRK LGLKI  HV
Sbjct: 363 NFLGMYLQAVPPSVLRPPKSEKAVRAMKKYQRQKDVRKLELRNARERNRKTLGLKIFRHV 422

Query: 432 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 491
            KK+K+++G K E +IE EV +IF++W +EV++DF  SLE+  +WH  L+ GDFLSL+HI
Sbjct: 423 LKKIKQSNGFKFEGEIENEVRDIFQSWGEEVMQDFMGSLEERWKWHWLLTRGDFLSLRHI 482

Query: 492 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEE--RYAKRTVEDLTRL 551
           R +LP DL++AYD FQ+QV++HL P +AK  K  EDEE+R+ EEE  RYA+RTVEDLT+L
Sbjct: 483 REKLPQDLIDAYDEFQEQVDKHLAPTQAK--KVLEDEERRVEEEEEQRYAERTVEDLTKL 542

Query: 552 CIKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCH 611
           C+KV AP ELVRKA+K++GFTN MGRPRPI  L+ LED+DIIKWYA              
Sbjct: 543 CMKVSAPEELVRKAIKLVGFTNSMGRPRPIIHLVTLEDSDIIKWYA-------------- 602

Query: 612 NYKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVK 671
                                 EKH STK+  ++HY+KDL+V DL+G+EE HFP+EREVK
Sbjct: 603 --------------------RHEKHGSTKK-LIRHYTKDLRVSDLDGREEAHFPSEREVK 662

Query: 672 MLGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPS 731
           M+G++NL+DP PVDG  SL LIRL +DE  + C A FC R+D+I++RV LLQ  L++NP 
Sbjct: 663 MMGDKNLSDPKPVDGTLSLLLIRLASDEPLHHCAASFCERSDTIMHRVHLLQNRLHINPL 722

Query: 732 NGVEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDM 768
           +  +WV GMG IH +LN++CLPLC+ HI+D+Y+GKI LQD+D +  +D+
Sbjct: 723 DEEKWVPGMGTIHSALNRKCLPLCSTHISDVYLGKITLQDVDSSSFIDL 722

BLAST of Carg00070 vs. ExPASy Swiss-Prot
Match: Q9CA78 (Nuclear intron maturase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT4 PE=3 SV=2)

HSP 1 Score: 223.8 bits (569), Expect = 6.7e-57
Identity = 198/753 (26.29%), Postives = 338/753 (44.89%), Query Frame = 0

Query: 50  QLEALVLSRFSQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSC 109
           +L+  V  +   GKF DLL+ V+A P  L  A   +       RLN   S +S+   +  
Sbjct: 90  RLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCI-------RLN---SNVSITERNGS 149

Query: 110 FSVEDMARELYENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERF 169
            + + +A EL    FDV +    +   ++  E LVLP++ LKV+ EAIR+VLE+V+   F
Sbjct: 150 VAFDSIAEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIRIVLEVVFSPHF 209

Query: 170 VTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDI 229
              S+  R G GR +A++Y+ N++    W FT++  ++   SV  N L ++ +EK++D  
Sbjct: 210 SKISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLSVM-EEKVEDSS 269

Query: 230 LILMLKKLFELEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRL------ 289
           L ++L+ +FE   + +E GG   G G PQE  L  +L NIY + FD E  +I +      
Sbjct: 270 LSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEFYRISMRHEALG 329

Query: 290 ---QKNEENP-------------KFSLDGTVSFHNPVKIYAVRYLDEILVITSGSKMLIM 349
              + +E++P             +  L  T      +++Y  R++DEI    SG K +  
Sbjct: 330 LDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVALRVYCCRFMDEIYFSVSGPKKVAS 389

Query: 350 ELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRAR 409
           +++S+ + +L  +L L++           +  +  LG     V  +V   P + KA+   
Sbjct: 390 DIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG---TLVRKNVRESP-TVKAVHKL 449

Query: 410 KKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKRTD--GL--------------K 469
           K+ +R   + A++   A      ++G K L H  KK+K ++  GL              K
Sbjct: 450 KEKVR---LFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKGLADSNSTLSQISCHRK 509

Query: 470 SEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKH-IRNQLPVDLVN 529
           +  + +     + R W ++V+R    +  D +E        +F+  KH +   +P +L +
Sbjct: 510 AGMETDHWYKILLRIWMEDVLR----TSADRSE--------EFVLSKHVVEPTVPQELRD 569

Query: 530 AYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCIKVEAPIELVR 589
           A+ +FQ+    +++   A  E      +               D       V AP   + 
Sbjct: 570 AFYKFQNAAAAYVSSETANLEALLPCPQS-------------HDRPVFFGDVVAPTNAIG 629

Query: 590 KAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYH 649
           + +   G     G  R  S LI L+   II WY+G+ RRW+ ++  C N+  +K ++   
Sbjct: 630 RRLYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDEIKALIDNQ 689

Query: 650 LRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKMLGERNLADPYP 709
           +R SCI TLA K+   + E  K    +L         E     E+      +R+    Y 
Sbjct: 690 IRMSCIRTLAAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSPAFDRDEHLTYG 749

Query: 710 V--DGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNGVEWVRGMG 761
           +   G   L L RLV++     C    C+     +Y +  +++           W  G  
Sbjct: 750 LSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------QKFPGWKTGFS 793

BLAST of Carg00070 vs. ExPASy Swiss-Prot
Match: P03876 (Putative COX1/OXI3 intron 2 protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AI2 PE=4 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 1.7e-12
Identity = 74/277 (26.71%), Postives = 127/277 (45.85%), Query Frame = 0

Query: 132 RLEPSEEKGEF--LVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYL 191
           R+E  +  G F  L + N + K++ E++RM+LEI+Y+  F  +S+G R  +   TAI   
Sbjct: 345 RVEIPKTSGGFRPLSVGNPREKIVQESMRMMLEIIYNNSFSYYSHGFRPNLSCLTAIIQC 404

Query: 192 KNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGG 251
           KN +Q  +W+  V    + FD++  N L  ++ E+IKD   + +L KL  L A  ++   
Sbjct: 405 KNYMQYCNWFIKVDL-NKCFDTIPHNMLINVLNERIKDKGFMDLLYKL--LRAGYVDKNN 464

Query: 252 CYLGR--GFPQESGLCSILSNIYF--------NGFDKEIQQIRLQKNEENPKF-SLDGTV 311
            Y     G PQ S +  IL NI+         N F+ E     +     NP + SL   +
Sbjct: 465 NYHNTTLGIPQGSVVSPILCNIFLDKLDKYLENKFENEFNTGNMSNRGRNPIYNSLSSKI 524

Query: 312 ------------------------SFHNPVKIYAVRYLDEILVITSGSKMLIMELKSQVL 371
                                   S  +  + Y VRY D+I++   GS      + + + 
Sbjct: 525 YRCKLLSEKLKLIRLRDHYQRNMGSDKSFKRAYFVRYADDIIIGVMGSHNDCKNILNDIN 584


HSP 2 Score: 53.5 bits (127), Expect = 1.2e-05
Identity = 53/221 (23.98%), Postives = 104/221 (47.06%), Query Frame = 0

Query: 542 VEAPIELVRKAVKMIGFTNK--MGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHN 601
           V API  +   +   G+ +   +G+PR +  LI  E   I+  Y  VGR  ++++    N
Sbjct: 643 VNAPIRSIVMKLNKHGYCSHGILGKPRGVGRLIHEEMKTILMHYLAVGRGIMNYYRLATN 702

Query: 602 YKMVKTIVTYHLRFSCILTLAEKHE-STKREAMKHYSKDLKVFDLNGKEEMHFPTEREVK 661
           +  ++  +TY L +SC LTLA K + +T ++ +  + K L    ++   ++ F  + + K
Sbjct: 703 FTTLRGRITYILFYSCCLTLARKFKLNTVKKVILKFGKVL----VDPHSKVSFSID-DFK 762

Query: 662 MLGERNLADP-YPVDGAFSLFLIRLVTDEDSYPCIAHFC-NRTDSILYRVRLLQKTLNVN 721
           +  + N+ D  Y  D     +   L      +  I   C ++ D  ++ VR    TLN N
Sbjct: 763 IRHKMNMTDSNYTPDEILDRYKYMLPRSLSLFSGICQICGSKHDLEVHHVR----TLN-N 822

Query: 722 PSNGV--EWVRGMGVIHESLNQRCLPLCADHINDLYMGKIN 756
            +N +  +++ G  +    +N++ + +C      ++ GK N
Sbjct: 823 AANKIKDDYLLGRMI---KMNRKQITICKTCHFKVHQGKYN 850

BLAST of Carg00070 vs. ExPASy Swiss-Prot
Match: B1N1A3 (Putative nicotine oxidoreductase OS=Pseudomonas putida OX=303 GN=nicA PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.7e-12
Identity = 72/264 (27.27%), Postives = 120/264 (45.45%), Query Frame = 0

Query: 151 KVLLEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRRRKFD 210
           KV+ E IR +LE +Y+  F   S+G R G   HTA++ ++ S    +W       +  FD
Sbjct: 109 KVVQEVIRSILEAIYEPTFSKNSHGFRAGKSCHTALKQVRESWSGVTWVIEGDI-KGCFD 168

Query: 211 SVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGGCYLGR-GFPQESGLCSILSNI 270
           ++  +KL   ++ +IKD+  I +++K   L A   E G  +    G PQ S +  IL+N+
Sbjct: 169 NISHSKLIDQLRLRIKDERFINLIRK--ALNAGYFENGAFFSATLGTPQGSIISPILANV 228

Query: 271 YFNGFDKEIQQI-------------------RLQ--------KNEENPKFSLDGTVSF-- 330
           + +  D++++Q+                   +LQ        K E+      D T+S   
Sbjct: 229 FLDQLDRKVEQLIKDHHQGEEGDKITDPAYRKLQRQKTSLRKKAEKQEGAERDATLSLAR 288

Query: 331 ----------------HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELE 369
                           +  +++  VRY D+ ++  +G K+L  EL+S V  +LE N  LE
Sbjct: 289 EANSKLLSMSPYLTRNNGFIRVKYVRYADDWIIGVNGPKLLAEELRSVVGEFLE-NAGLE 348

BLAST of Carg00070 vs. ExPASy Swiss-Prot
Match: P05511 (Uncharacterized 91 kDa protein in cob intron OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPMIT.06 PE=4 SV=4)

HSP 1 Score: 70.1 bits (170), Expect = 1.2e-10
Identity = 65/252 (25.79%), Postives = 113/252 (44.84%), Query Frame = 0

Query: 151 KVLLEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRRRKFD 210
           K++ E +R+VLE +Y+  F T S+G R G   H+A+R +  + +  +WW      +  FD
Sbjct: 318 KLVQEILRIVLEAIYEPLFNTASHGFRPGRSCHSALRSIFTNFKGCTWWIEGDI-KACFD 377

Query: 211 SVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQIELGGCYLGRGFPQESGLCSILSNIY 270
           S+  +KL  L+  KIKD   I +++K         E    Y   G PQ S +  IL+NIY
Sbjct: 378 SIPHDKLIALLSSKIKDQRFIQLIRKALN-AGYLTENRYKYDIVGTPQGSIVSPILANIY 437

Query: 271 FNGFDKEIQQIRLQKNEENPKFSLDGTVSFH----------------------------- 330
            +  D+ I+ ++ + + + P      + S H                             
Sbjct: 438 LHQLDEFIENLKSEFDYKGPIARKRTSESRHLHYLMAKAKRENADSKTIRKIAIEMRNVP 497

Query: 331 NPV------KIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSA 368
           N +      K+  VRY D+ +V  +GS     E+ +++  +   ++ L V    T I ++
Sbjct: 498 NKIHGIQSNKLMYVRYADDWIVAVNGSYTQTKEILAKITCFC-SSIGLTVSPTKTKITNS 557

BLAST of Carg00070 vs. ExPASy TrEMBL
Match: A0A6J1FV64 (nuclear intron maturase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111447574 PE=4 SV=1)

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 760/768 (98.96%), Postives = 764/768 (99.48%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60
           MLIHLRKIATPLKPKLSSSFNKLYSD PLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSFNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120
           QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAP+SLLSLDMVSSCFSVEDMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSLDMVSSCFSVEDMARELY 120

Query: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180
           ENRFDVGACCVRLE SEEKGEFLVLPNLKLKVLLEAIR+VLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300
           EAVQIELGGCYLGRG PQESGLCSILSNIYFNGFDKEIQQIRL+KNEENPKFSLDGTVSF
Sbjct: 241 EAVQIELGGCYLGRGLPQESGLCSILSNIYFNGFDKEIQQIRLEKNEENPKFSLDGTVSF 300

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAV PSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVLPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480
           FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540
           RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660
           KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720
           GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHI+DLYMGKINLQDLDCTLSLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Carg00070 vs. ExPASy TrEMBL
Match: A0A6J1ILC2 (nuclear intron maturase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111476629 PE=4 SV=1)

HSP 1 Score: 1495.7 bits (3871), Expect = 0.0e+00
Identity = 756/768 (98.44%), Postives = 763/768 (99.35%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60
           MLIHLRKIATPLKPKLSSS NKLYSD PLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSLNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120
           QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120

Query: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180
           ENRFDVGACCVRLE SEEKGEFLVLPNLKLKVLLEA++MVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAMKMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300
           EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSL+GTVSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLNGTVSF 300

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           HNPVKIYAVRYLDEILVITSGSKM IMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMQIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480
           FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540
           RNQLPVDLVNAYDRFQDQVN+HLNPLKAK+EKAREDEEKRL EEERYAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNKHLNPLKAKKEKAREDEEKRLVEEERYAKRTVEDLTRLCI 540

Query: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KVEAPIELVRKAVKMIGFTNKMGRP+PISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPQPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660
           KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720
           GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHI+DLYMGKINLQDLDCTLSLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Carg00070 vs. ExPASy TrEMBL
Match: A0A6J1DNH6 (nuclear intron maturase 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111022853 PE=4 SV=1)

HSP 1 Score: 1309.7 bits (3388), Expect = 0.0e+00
Identity = 662/770 (85.97%), Postives = 711/770 (92.34%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHS-PLLKLPFQHSVQTLTRPQLEALVLSRF 60
           M I LRKIATPLK  LS+SFNKLYSD PLKHS PL   PFQHS +TLT P+L+ALVLSRF
Sbjct: 1   MFIPLRKIATPLKSILSTSFNKLYSDLPLKHSPPLHNFPFQHSTETLTWPELKALVLSRF 60

Query: 61  SQGKFFDLLQNVVASPSVLFTASQNLIT-PLPSNRLNAPESLLSLDMVSSCFSVEDMARE 120
           + GKF DLLQNVVASPSVL TASQNLIT P PSN LNAP+ L  LD+VS CFSVE+MARE
Sbjct: 61  THGKFLDLLQNVVASPSVLLTASQNLITPPPPSNGLNAPDPLPILDLVSKCFSVEEMARE 120

Query: 121 LYENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRV 180
           LYE+RFDVGACCVR+   ++KG+ LVLPNLKLKVL+EAIRMVLEIVYDERFVTFSYGGRV
Sbjct: 121 LYEDRFDVGACCVRM---DQKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRV 180

Query: 181 GMGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLF 240
           GMGRHTAIRYLKNSVQNPSWWFTVAFRR+KFDSVHV+KLCLLV+EKIKD +LI MLKKLF
Sbjct: 181 GMGRHTAIRYLKNSVQNPSWWFTVAFRRKKFDSVHVHKLCLLVEEKIKDHLLICMLKKLF 240

Query: 241 ELEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTV 300
           ELEA+QIELG CYLGRGFPQESGLCSIL NI+FNGFDK+IQQIRLQKNEENPKFSLD  V
Sbjct: 241 ELEAIQIELGACYLGRGFPQESGLCSILCNIFFNGFDKDIQQIRLQKNEENPKFSLDEIV 300

Query: 301 SFHNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSE 360
           SFH+PVKIYAVRYLDEILVITSGSKML M+LKSQVL+YLEGNLELEVDRMNTAIHSAVSE
Sbjct: 301 SFHSPVKIYAVRYLDEILVITSGSKMLTMDLKSQVLKYLEGNLELEVDRMNTAIHSAVSE 360

Query: 361 KISFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILS 420
           KISFLGMELQAVPPSVLHPPMSEKAIRARKKY+RQKEVR IELRNARERNRKKLGLKILS
Sbjct: 361 KISFLGMELQAVPPSVLHPPMSEKAIRARKKYIRQKEVRVIELRNARERNRKKLGLKILS 420

Query: 421 HVFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLK 480
           HVFKKLK+TDG K EFQIEKEV EIFRNWADEV + FFESLE++ EWH  LSAGDFLSLK
Sbjct: 421 HVFKKLKQTDGFKFEFQIEKEVREIFRNWADEVAQHFFESLENHAEWHHALSAGDFLSLK 480

Query: 481 HIRNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRL 540
           HIRNQLP DLVNAYDRFQDQV++HLNP+K K  KAREDEEKR+ EE++YA+RTVEDLTRL
Sbjct: 481 HIRNQLPEDLVNAYDRFQDQVDKHLNPVKVKYVKAREDEEKRVEEEQKYARRTVEDLTRL 540

Query: 541 CIKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCH 600
           CIKV+APIELVRKAVKM+GFTNKMGRP+PIS L+ALED DIIKWYAGVGRRWLDFFCCCH
Sbjct: 541 CIKVDAPIELVRKAVKMVGFTNKMGRPQPISLLVALEDIDIIKWYAGVGRRWLDFFCCCH 600

Query: 601 NYKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVK 660
           NYKMVKT+VTYHLRFSCILTLAEKHESTKREAMKH+SKDLKVFDLNG EE+HFPTEREVK
Sbjct: 601 NYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKHFSKDLKVFDLNGDEEIHFPTEREVK 660

Query: 661 MLGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPS 720
           MLG+R LADPYPVDG  SLFLIRL  DE S PCIAHFCNRTDSILYRVRLLQ+TLNVN  
Sbjct: 661 MLGDRMLADPYPVDGTLSLFLIRLAIDEPSCPCIAHFCNRTDSILYRVRLLQRTLNVNSC 720

Query: 721 NGVEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           +G +WVRGMGVIHESLNQRCLPLCADHI+DLYMG+INLQDLDCTLSLDMD
Sbjct: 721 DGEKWVRGMGVIHESLNQRCLPLCADHISDLYMGRINLQDLDCTLSLDMD 767

BLAST of Carg00070 vs. ExPASy TrEMBL
Match: A0A1S4E4Y1 (uncharacterized protein LOC103501588 OS=Cucumis melo OX=3656 GN=LOC103501588 PE=4 SV=1)

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 666/768 (86.72%), Postives = 704/768 (91.67%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60
           M IH RKIAT LKPK S+S NKLYS  PL+   +L LP QHS ++LT  QL+ALVLSRFS
Sbjct: 1   MFIHFRKIATSLKPKFSNSLNKLYSHLPLR-KKVLNLPSQHSPESLTSSQLKALVLSRFS 60

Query: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120
            GKF DL QNVVASPSVL TAS+NLITP  S   NAP+SL   D+VS CFSVE MAREL 
Sbjct: 61  HGKFVDLFQNVVASPSVLLTASRNLITPPFS---NAPDSLPLSDLVSKCFSVEVMARELS 120

Query: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180
           ENRFDVGACCV + P EEKGE LVLPNLKLKVL+EAIRMVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWFTVAFRR+KF+SVHVN LCLL+QEKIKDDILI ML+KLFE+
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEV 240

Query: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300
           EA+QIELGGCYLGRGFPQESGLCSIL NIYFNGFDKEIQ+IRLQKNEENPKF+LD  VSF
Sbjct: 241 EAIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSF 300

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           HNPVKIYAVRYLDEILVITSGSKML MELKSQVL+YLEGNLELEVDRMNTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
            FLGMELQAV PSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSH+
Sbjct: 361 GFLGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHM 420

Query: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480
           FKK KRT G KSEFQIE EV  IFRNWADEVV+DFFES ED+ EWHR LSAGDFLSLKHI
Sbjct: 421 FKKFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDHAEWHRVLSAGDFLSLKHI 480

Query: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540
           RNQLP DLVNAYDRFQ QVN+HLNP+K K+EKAREDEEKRL EEE YAKRTVEDLTRLCI
Sbjct: 481 RNQLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCI 540

Query: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KV+APIELVRKAV+M+GFTNKMGRPRPISSLIALED DIIKWY+GVGRRWLDFFCCCHNY
Sbjct: 541 KVDAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660
           KMVKT+VTYHLRFSCILTLAEKHESTKREAMKHY KDLKVFDLNG EEMHFPTE+ VKML
Sbjct: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKML 660

Query: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720
           GERNLADPYPVDGA SL LIRL TDE SYPCIA+FCNRT+SILYRVRLLQ+TLNVNPS+G
Sbjct: 661 GERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           VEWV+GMGVIHESLNQRCLPLCADHI+DLYMGKINLQDLDCTLSLDMD
Sbjct: 721 VEWVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

BLAST of Carg00070 vs. ExPASy TrEMBL
Match: A0A5A7SWY5 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold400G00320 PE=4 SV=1)

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 667/768 (86.85%), Postives = 703/768 (91.54%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60
           M IH RKIAT LKPK S+S NKLYS  PL+   +L LP QHS ++LT  QL+ALVLSRFS
Sbjct: 1   MFIHFRKIATSLKPKFSNSLNKLYSHLPLR-KKVLNLPSQHSPESLTSSQLKALVLSRFS 60

Query: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120
            GKF DL QNVVASPSVL TAS+NLITP  S   NAP+SL   D+VS CFSVE MAREL 
Sbjct: 61  HGKFVDLFQNVVASPSVLLTASRNLITPPFS---NAPDSLPLSDLVSKCFSVEVMARELS 120

Query: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180
           ENRFDVGACCV + P EEKGE LVLPNLKLKVL+EAIRMVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWFTVAFRR+KF+SVHVN LCLL+QEKIKDDILI ML+KLFE+
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEV 240

Query: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300
           EA+QIELGGCYLGRGFPQESGLCSIL NIYFNGFDKEIQ+IRLQKNEENPKF+LD  VSF
Sbjct: 241 EAIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSF 300

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           HNPVKIYAVRYLDEILVITSGSKML MELKSQVL+YLEGNLELEVDRMNTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
            FLGMELQAV PSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 GFLGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480
           FKK KRT G KSEFQIE EV  IFRNWADEVV+DFFES ED  EWHR LSAGDFLSLKHI
Sbjct: 421 FKKFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDPAEWHRVLSAGDFLSLKHI 480

Query: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540
           RNQLP DLVNAYDRFQ QVN+HLNP+K K+EKAREDEEKRL EEE YAKRTVEDLTRLCI
Sbjct: 481 RNQLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCI 540

Query: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KV+APIELVRKAV+M+GFTNKMGRPRPISSLIALED DIIKWY+GVGRRWLDFFCCCHNY
Sbjct: 541 KVDAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660
           KMVKT+VTYHLRFSCILTLAEKHESTKREAMKHY KDLKVFDLNG EEMHFPTE+ VKML
Sbjct: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKML 660

Query: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720
           GERNLADPYPVDGA SL LIRL TDE SYPCIA+FCNRT+SILYRVRLLQ+TLNVNPS+G
Sbjct: 661 GERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 769
           VEWV+GMGVIHESLNQRCLPLCADHI+DLYMGKINLQDLDCTLSLDMD
Sbjct: 721 VEWVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

BLAST of Carg00070 vs. TAIR 10
Match: AT5G04050.2 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 717.6 bits (1851), Expect = 1.1e-206
Identity = 403/769 (52.41%), Postives = 508/769 (66.06%), Query Frame = 0

Query: 12  LKPKLSSSFNKLYS---DSPLKHSPLLKLPFQHSVQTLTRP----QLEALVLSRFSQGKF 71
           L+ ++ S +N+  S    S L++       F +S QT+T P    +LEALVL ++S GKF
Sbjct: 3   LRLRVHSFYNRGISFLVSSSLRNLSTASSLFLNSDQTITEPLVKSELEALVLKQYSHGKF 62

Query: 72  FDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELYENRF 131
           + L++N V+ P VL  A QNL        L+A  S    D VS  FS+E+M RE+ E RF
Sbjct: 63  YSLVKNAVSLPCVLLAACQNL-------SLSANSSGDLADRVSRRFSIEEMGREIREGRF 122

Query: 132 DVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGMGRHT 191
           D+ +CCV    S      LVLPNLKLKVL+EAIRMVLEIVYD+RF TFSYGGRVGMGRHT
Sbjct: 123 DIRSCCVEFISSS-----LVLPNLKLKVLIEAIRMVLEIVYDDRFATFSYGGRVGMGRHT 182

Query: 192 AIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQ 251
           AIRYLKNSV+NP WWF V+F R  F+  +V+ LC  V EKI D +LI M+KKLFE   ++
Sbjct: 183 AIRYLKNSVENPRWWFRVSFAREMFEERNVDILCGFVGEKINDVMLIEMIKKLFEFGILK 242

Query: 252 IELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVS----F 311
           IELGGC  GRGFPQE GLCSIL N+YF+G DKEIQ +RL+   +NP+       S    F
Sbjct: 243 IELGGCNSGRGFPQECGLCSILINVYFDGLDKEIQDLRLKMKVKNPRVGTGDEESTGNVF 302

Query: 312 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 371
             PV IYAVRYLDEILVITSGSKML M+LK +++  LE  LEL VDR+NT+IHSAVSEKI
Sbjct: 303 FKPVNIYAVRYLDEILVITSGSKMLTMDLKKRIVDILEQRLELRVDRLNTSIHSAVSEKI 362

Query: 372 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 431
           +FLGM LQAVPPSVL PP SEKA+RA KKY RQK+VR +ELRNARERNRK LGLKI  HV
Sbjct: 363 NFLGMYLQAVPPSVLRPPKSEKAVRAMKKYQRQKDVRKLELRNARERNRKTLGLKIFRHV 422

Query: 432 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 491
            KK+K+++G K E +IE EV +IF++W +EV++DF  SLE+  +WH  L+ GDFLSL+HI
Sbjct: 423 LKKIKQSNGFKFEGEIENEVRDIFQSWGEEVMQDFMGSLEERWKWHWLLTRGDFLSLRHI 482

Query: 492 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEE--RYAKRTVEDLTRL 551
           R +LP DL++AYD FQ+QV++HL P +AK  K  EDEE+R+ EEE  RYA+RTVEDLT+L
Sbjct: 483 REKLPQDLIDAYDEFQEQVDKHLAPTQAK--KVLEDEERRVEEEEEQRYAERTVEDLTKL 542

Query: 552 CIKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCH 611
           C+KV AP ELVRKA+                                             
Sbjct: 543 CMKVSAPEELVRKAI--------------------------------------------- 602

Query: 612 NYKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVK 671
                                                   KV DL+G+EE HFP+EREVK
Sbjct: 603 ----------------------------------------KVSDLDGREEAHFPSEREVK 662

Query: 672 MLGERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPS 731
           M+G++NL+DP PVDG  SL LIRL +DE  + C A FC R+D+I++RV LLQ  L++NP 
Sbjct: 663 MMGDKNLSDPKPVDGTLSLLLIRLASDEPLHHCAASFCERSDTIMHRVHLLQNRLHINPL 672

Query: 732 NGVEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDM 768
           +  +WV GMG IH +LN++CLPLC+ HI+D+Y+GKI LQD+D +  +D+
Sbjct: 723 DEEKWVPGMGTIHSALNRKCLPLCSTHISDVYLGKITLQDVDSSSFIDL 672

BLAST of Carg00070 vs. TAIR 10
Match: AT5G04050.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 691.8 bits (1784), Expect = 6.2e-199
Identity = 372/609 (61.08%), Postives = 456/609 (74.88%), Query Frame = 0

Query: 12  LKPKLSSSFNKLYS---DSPLKHSPLLKLPFQHSVQTLTRP----QLEALVLSRFSQGKF 71
           L+ ++ S +N+  S    S L++       F +S QT+T P    +LEALVL ++S GKF
Sbjct: 3   LRLRVHSFYNRGISFLVSSSLRNLSTASSLFLNSDQTITEPLVKSELEALVLKQYSHGKF 62

Query: 72  FDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELYENRF 131
           + L++N V+ P VL  A QNL        L+A  S    D VS  FS+E+M RE+ E RF
Sbjct: 63  YSLVKNAVSLPCVLLAACQNL-------SLSANSSGDLADRVSRRFSIEEMGREIREGRF 122

Query: 132 DVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGMGRHT 191
           D+ +CCV    S      LVLPNLKLKVL+EAIRMVLEIVYD+RF TFSYGGRVGMGRHT
Sbjct: 123 DIRSCCVEFISSS-----LVLPNLKLKVLIEAIRMVLEIVYDDRFATFSYGGRVGMGRHT 182

Query: 192 AIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFELEAVQ 251
           AIRYLKNSV+NP WWF V+F R  F+  +V+ LC  V EKI D +LI M+KKLFE   ++
Sbjct: 183 AIRYLKNSVENPRWWFRVSFAREMFEERNVDILCGFVGEKINDVMLIEMIKKLFEFGILK 242

Query: 252 IELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVS----F 311
           IELGGC  GRGFPQE GLCSIL N+YF+G DKEIQ +RL+   +NP+       S    F
Sbjct: 243 IELGGCNSGRGFPQECGLCSILINVYFDGLDKEIQDLRLKMKVKNPRVGTGDEESTGNVF 302

Query: 312 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 371
             PV IYAVRYLDEILVITSGSKML M+LK +++  LE  LEL VDR+NT+IHSAVSEKI
Sbjct: 303 FKPVNIYAVRYLDEILVITSGSKMLTMDLKKRIVDILEQRLELRVDRLNTSIHSAVSEKI 362

Query: 372 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 431
           +FLGM LQAVPPSVL PP SEKA+RA KKY RQK+VR +ELRNARERNRK LGLKI  HV
Sbjct: 363 NFLGMYLQAVPPSVLRPPKSEKAVRAMKKYQRQKDVRKLELRNARERNRKTLGLKIFRHV 422

Query: 432 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 491
            KK+K+++G K E +IE EV +IF++W +EV++DF  SLE+  +WH  L+ GDFLSL+HI
Sbjct: 423 LKKIKQSNGFKFEGEIENEVRDIFQSWGEEVMQDFMGSLEERWKWHWLLTRGDFLSLRHI 482

Query: 492 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEE--RYAKRTVEDLTRL 551
           R +LP DL++AYD FQ+QV++HL P +AK  K  EDEE+R+ EEE  RYA+RTVEDLT+L
Sbjct: 483 REKLPQDLIDAYDEFQEQVDKHLAPTQAK--KVLEDEERRVEEEEEQRYAERTVEDLTKL 542

Query: 552 CIKVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCH 608
           C+KV AP ELVRKA+K++GFTN MGRPRPI  L+ LED+DIIKWYAGVGR+WLDFFCCCH
Sbjct: 543 CMKVSAPEELVRKAIKLVGFTNSMGRPRPIIHLVTLEDSDIIKWYAGVGRKWLDFFCCCH 597

BLAST of Carg00070 vs. TAIR 10
Match: AT1G74350.1 (Intron maturase, type II family protein )

HSP 1 Score: 223.8 bits (569), Expect = 4.8e-58
Identity = 198/753 (26.29%), Postives = 338/753 (44.89%), Query Frame = 0

Query: 50  QLEALVLSRFSQGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSC 109
           +L+  V  +   GKF DLL+ V+A P  L  A   +       RLN   S +S+   +  
Sbjct: 45  RLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCI-------RLN---SNVSITERNGS 104

Query: 110 FSVEDMARELYENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERF 169
            + + +A EL    FDV +    +   ++  E LVLP++ LKV+ EAIR+VLE+V+   F
Sbjct: 105 VAFDSIAEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIRIVLEVVFSPHF 164

Query: 170 VTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDI 229
              S+  R G GR +A++Y+ N++    W FT++  ++   SV  N L ++ +EK++D  
Sbjct: 165 SKISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLSVM-EEKVEDSS 224

Query: 230 LILMLKKLFELEAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRL------ 289
           L ++L+ +FE   + +E GG   G G PQE  L  +L NIY + FD E  +I +      
Sbjct: 225 LSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEFYRISMRHEALG 284

Query: 290 ---QKNEENP-------------KFSLDGTVSFHNPVKIYAVRYLDEILVITSGSKMLIM 349
              + +E++P             +  L  T      +++Y  R++DEI    SG K +  
Sbjct: 285 LDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVALRVYCCRFMDEIYFSVSGPKKVAS 344

Query: 350 ELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRAR 409
           +++S+ + +L  +L L++           +  +  LG     V  +V   P + KA+   
Sbjct: 345 DIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG---TLVRKNVRESP-TVKAVHKL 404

Query: 410 KKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKRTD--GL--------------K 469
           K+ +R   + A++   A      ++G K L H  KK+K ++  GL              K
Sbjct: 405 KEKVR---LFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKGLADSNSTLSQISCHRK 464

Query: 470 SEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKH-IRNQLPVDLVN 529
           +  + +     + R W ++V+R    +  D +E        +F+  KH +   +P +L +
Sbjct: 465 AGMETDHWYKILLRIWMEDVLR----TSADRSE--------EFVLSKHVVEPTVPQELRD 524

Query: 530 AYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCIKVEAPIELVR 589
           A+ +FQ+    +++   A  E      +               D       V AP   + 
Sbjct: 525 AFYKFQNAAAAYVSSETANLEALLPCPQS-------------HDRPVFFGDVVAPTNAIG 584

Query: 590 KAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTIVTYH 649
           + +   G     G  R  S LI L+   II WY+G+ RRW+ ++  C N+  +K ++   
Sbjct: 585 RRLYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDEIKALIDNQ 644

Query: 650 LRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKMLGERNLADPYP 709
           +R SCI TLA K+   + E  K    +L         E     E+      +R+    Y 
Sbjct: 645 IRMSCIRTLAAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSPAFDRDEHLTYG 704

Query: 710 V--DGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNGVEWVRGMG 761
           +   G   L L RLV++     C    C+     +Y +  +++           W  G  
Sbjct: 705 LSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------QKFPGWKTGFS 748

BLAST of Carg00070 vs. TAIR 10
Match: ATMG00520.1 (Intron maturase, type II family protein )

HSP 1 Score: 81.3 bits (199), Expect = 3.8e-15
Identity = 99/369 (26.83%), Postives = 166/369 (44.99%), Query Frame = 0

Query: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360
           H  ++I   RY D++L+   G+  L++E++ ++  +L+  L L V    +   +A S  +
Sbjct: 311 HYLIRICYARYADDLLLGIVGAVELLIEIQKRIAHFLQSGLNLWVGSAGSTTIAARS-TV 370

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKI-LSH 420
            FLG  ++ VPP    P    + +  R +   +  + A  LR+A     + LG  I +  
Sbjct: 371 EFLGTVIREVPPRTT-PIQFLRELEKRLRVKHRIHITACHLRSAIHSKFRNLGDSIPIKQ 430

Query: 421 VFKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWH-RPLSAGDFLSLK 480
           + K + +T  L+   Q+ + +           VR    S+   T  H R  S G  +S  
Sbjct: 431 LTKGMSKTGSLQDGVQLAETL-------GTAGVRSPQVSVLWGTVKHIRQGSRG--ISFL 490

Query: 481 HIRNQLPVDLVNAYDRFQDQVNQ---HLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDL 540
           H   +      NA    Q  V++   H   L       R    K  GE   +   ++   
Sbjct: 491 HSSGR-----SNASSDVQQVVSRSGTHARKLSLYTPPGR----KAAGEGGGHWAGSIS-- 550

Query: 541 TRLCIKVEAPIELVRKAVKMIGFTNKMGRPRPI--SSLIALEDTDIIKWYAGVGRRWLDF 600
           +   IK+EAPI+ + + ++  G  ++  RP PI  + L  + D DI+ W AG+    L +
Sbjct: 551 SEFPIKIEAPIKKILRRLRDRGIISRR-RPWPIHVACLTNVSDEDIVNWSAGIAISPLSY 610

Query: 601 FCCCHNYKMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDL-NGKEEMHFP 660
           + C  N   V+TIV + +R+S I TLA KH+S+    +  YSKD  + +   GK    FP
Sbjct: 611 YRCRDNLYQVRTIVDHQIRWSAIFTLAHKHKSSAPNIILKYSKDSNIVNQEGGKILAEFP 656

Query: 661 TEREVKMLG 662
              E+  LG
Sbjct: 671 NSIELGKLG 656

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031416.10.0e+00100.00Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp.... [more]
KAG6600779.10.0e+0099.74Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp.... [more]
XP_022942578.10.0e+0098.96nuclear intron maturase 3, mitochondrial [Cucurbita moschata][more]
XP_022976163.10.0e+0098.44nuclear intron maturase 3, mitochondrial [Cucurbita maxima][more]
XP_023544815.10.0e+0097.79nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544... [more]
Match NameE-valueIdentityDescription
Q9LZA51.1e-23256.70Nuclear intron maturase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT... [more]
Q9CA786.7e-5726.29Nuclear intron maturase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT... [more]
P038761.7e-1226.71Putative COX1/OXI3 intron 2 protein OS=Saccharomyces cerevisiae (strain ATCC 204... [more]
B1N1A31.7e-1227.27Putative nicotine oxidoreductase OS=Pseudomonas putida OX=303 GN=nicA PE=4 SV=1[more]
P055111.2e-1025.79Uncharacterized 91 kDa protein in cob intron OS=Schizosaccharomyces pombe (strai... [more]
Match NameE-valueIdentityDescription
A0A6J1FV640.0e+0098.96nuclear intron maturase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1ILC20.0e+0098.44nuclear intron maturase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A6J1DNH60.0e+0085.97nuclear intron maturase 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A1S4E4Y10.0e+0086.72uncharacterized protein LOC103501588 OS=Cucumis melo OX=3656 GN=LOC103501588 PE=... [more]
A0A5A7SWY50.0e+0086.85RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
Match NameE-valueIdentityDescription
AT5G04050.21.1e-20652.41RNA-directed DNA polymerase (reverse transcriptase) [more]
AT5G04050.16.2e-19961.08RNA-directed DNA polymerase (reverse transcriptase) [more]
AT1G74350.14.8e-5826.29Intron maturase, type II family protein [more]
ATMG00520.13.8e-1526.83Intron maturase, type II family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 506..526
NoneNo IPR availablePANTHERPTHR33642COX1/OXI3 INTRON 1 PROTEIN-RELATEDcoord: 33..764
NoneNo IPR availablePANTHERPTHR33642:SF4NUCLEAR INTRON MATURASE 3, MITOCHONDRIALcoord: 33..764
NoneNo IPR availableCDDcd01651RT_G2_introncoord: 143..367
e-value: 1.72368E-40
score: 146.577
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 144..367
e-value: 4.1E-10
score: 39.6
IPR024937Domain XPFAMPF01348Intron_maturas2coord: 541..648
e-value: 5.7E-16
score: 58.8
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 143..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00070-RACarg00070-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006315 homing of group II introns
biological_process GO:0090615 mitochondrial mRNA processing
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
biological_process GO:0006397 mRNA processing
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003964 RNA-directed DNA polymerase activity