Carg00054 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00054
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Thiamine thiazole synthase, chloroplastic
Location	Carg_Chr04: 5069145 .. 5070793 (+)
RNA-Seq Expression	Carg00054
Synteny	Carg00054

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCCCTTCCCCATCCCATCTCCTCTGTTTTCCTCTGCTTCTACAAAAATGGCTTCCATGGCTTCCACTCTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTCCTCCTTACTTGACTCCTCTTTCCATGGCACCCCTCTGGCCCCTCCTCCTTCTCTCCGCCTCAAACCCACCCCCGCCTCTTTCTCCATCTCTGCCTCCGCCTCTCAGCAGCAGCCTTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGATACTGATGTCGTCATCGTCGGCGCTGGCTCTGCTGGCCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGATATTCGCATCGCTATCATTGAGCAGTCCGTCTCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTATGCTTTTGATTTCCTTATATGCCATTTATTTTCCTCACCCTTACCCATAACGCCTCCTTTTCTTTCCTGTCTTTTAGTTTTGAATGCTCTGTTTTTTGTTCTTTGTAAGAAAATACCCACAATTAGATCATGCTAATTCAGTCATAAATTGGCTTAAGCCCAAGAATTTAAATACTCAATCAGTACAAGATCTAATTCTAACATCTTTAATCTGATGGGTTTGGGCAAATTTAATGTTTGGTTCATGTTCTTTGATAGGTGGTAAGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGACCCTAATGTCATGGAGGCGAAGGTAGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGACTTAAGAGCATTGGCATGATCGATTCCGTCCCGGGAATGAAGGCCCTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTGACCCGAGAGGTGGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGTAAAGAACTAAGTTTAAATTAATGGTTTAACTAAAAAGCTCAGACCTTTTTTAACAATCAGAGGTGGTGCTGGATGCAGGGGCCAACGTTCGGGGCAATGATGATATCGGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGCAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCACCGGAGGTTGCAGATGGTTGAATGGGTGAGTTTTCATGGATGGAAGGCGGCGGTTGTGTTTTGTGATTCATGGGGGCTCTGTTTCCGTGGCAAACAGATTGGGGTTTGGTGAAAAGCCAATTTTAAGTTAAATTAAATAAAATTGTATCAAAATGTTAATTGCCTTTTTCTTGTTAAATTATTATAAAATTAGATTTAAATTAATAATTTTGTAGTTAATAAA

mRNA sequence

CGCCCTTCCCCATCCCATCTCCTCTGTTTTCCTCTGCTTCTACAAAAATGGCTTCCATGGCTTCCACTCTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTCCTCCTTACTTGACTCCTCTTTCCATGGCACCCCTCTGGCCCCTCCTCCTTCTCTCCGCCTCAAACCCACCCCCGCCTCTTTCTCCATCTCTGCCTCCGCCTCTCAGCAGCAGCCTTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGATACTGATGTCGTCATCGTCGGCGCTGGCTCTGCTGGCCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGATATTCGCATCGCTATCATTGAGCAGTCCGTCTCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTAAGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGACCCTAATGTCATGGAGGCGAAGGTAGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGACTTAAGAGCATTGGCATGATCGATTCCGTCCCGGGAATGAAGGCCCTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTGACCCGAGAGGTGGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCAATGATGATATCGGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGCAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCACCGGAGGTTGCAGATGGTTGAATGGGTGAGTTTTCATGGATGGAAGGCGGCGGTTGTGTTTTGTGATTCATGGGGGCTCTGTTTCCGTGGCAAACAGATTGGGGTTTGGTGAAAAGCCAATTTTAAGTTAAATTAAATAAAATTGTATCAAAATGTTAATTGCCTTTTTCTTGTTAAATTATTATAAAATTAGATTTAAATTAATAATTTTGTAGTTAATAAA

Coding sequence (CDS)

ATGGCTTCCATGGCTTCCACTCTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTCCTCCTTACTTGACTCCTCTTTCCATGGCACCCCTCTGGCCCCTCCTCCTTCTCTCCGCCTCAAACCCACCCCCGCCTCTTTCTCCATCTCTGCCTCCGCCTCTCAGCAGCAGCCTTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGATACTGATGTCGTCATCGTCGGCGCTGGCTCTGCTGGCCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGATATTCGCATCGCTATCATTGAGCAGTCCGTCTCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTAAGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGACCCTAATGTCATGGAGGCGAAGGTAGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGACTTAAGAGCATTGGCATGATCGATTCCGTCCCGGGAATGAAGGCCCTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTGACCCGAGAGGTGGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCAATGATGATATCGGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGCAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCACCGGAGGTTGCAGATGGTTGA

Protein sequence

MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASFSISASASQQQPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG

Homology

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BLAST of Carg00054 vs. NCBI nr
Match: KAG7031400.1 (Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681.4 bits (1757), Expect = 4.1e-192
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASFSISASASQQQPYD 60
           MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASFSISASASQQQPYD
Sbjct: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASFSISASASQQQPYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120
           LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI
Sbjct: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 352

[Close]

BLAST of Carg00054 vs. NCBI nr
Match: KAG6600763.1 (Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 674.5 bits (1739), Expect = 5.0e-190
Identity = 351/353 (99.43%), Postives = 351/353 (99.43%), Query Frame = 0


Query: 1   MASMASTLTTKLQTP-SSSSSSLLDSSFHGTPLAPPPSLRLKPTPASFSISASASQQQPY 60
           MASMASTLT KLQTP SSSSSSLLDSSFHGTPLAPPPSLRLKPTPASFSISASASQQQPY
Sbjct: 1   MASMASTLTIKLQTPSSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASFSISASASQQQPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA
Sbjct: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353

[Close]

BLAST of Carg00054 vs. NCBI nr
Match: XP_023524627.1 (thiamine thiazole synthase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 668.3 bits (1723), Expect = 3.6e-188
Identity = 349/353 (98.87%), Postives = 350/353 (99.15%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLA-PPPSLRLKPTPASFSISASASQQQPY 60
           MASMASTLTTKLQTP  SSSSLLDSSFHGTPLA PPPSLRLKPTPASFSISAS+SQQQPY
Sbjct: 1   MASMASTLTTKLQTP--SSSSLLDSSFHGTPLAPPPPSLRLKPTPASFSISASSSQQQPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA
Sbjct: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 351

[Close]

BLAST of Carg00054 vs. NCBI nr
Match: XP_022990741.1 (thiamine thiazole synthase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 666.4 bits (1718), Expect = 1.4e-187
Identity = 347/355 (97.75%), Postives = 348/355 (98.03%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLA---PPPSLRLKPTPASFSISASASQQQ 60
           MASMAST TTKLQTPSSSSSSLLDSSFHGTPLA   PPPSLRLK TPA+FSISASASQ Q
Sbjct: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGL NCIEDEAADLILAAGESPEVADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 355

[Close]

BLAST of Carg00054 vs. NCBI nr
Match: XP_022943248.1 (thiamine thiazole synthase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 654.1 bits (1686), Expect = 7.0e-184
Identity = 344/353 (97.45%), Postives = 346/353 (98.02%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLA-PPPSLRLKPTPASFSISASASQQQPY 60
           MAS+ASTLTTKLQTP   SSSLLDSSFHGTPLA PPPSLRLK TPA+FSI  SASQQQPY
Sbjct: 1   MASVASTLTTKLQTP---SSSLLDSSFHGTPLAPPPPSLRLKSTPAAFSI--SASQQQPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA
Sbjct: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 348

[Close]

BLAST of Carg00054 vs. ExPASy Swiss-Prot
Match: O23787 (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.1e-155
Identity = 295/360 (81.94%), Postives = 320/360 (88.89%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKP----TPASFSISASASQQ 60
           MA+MAST      + SS+++ L DSSFHG P++ P  LRL+P     P + SISASAS  
Sbjct: 1   MAAMASTAFA--PSVSSTTNKLFDSSFHGAPMS-PSLLRLQPIKSSRPNNLSISASAS-- 60

Query: 61  QPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDI 120
            PYDLN+FKF+PIKESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSKNP+I
Sbjct: 61  PPYDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNI 120

Query: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTST 180
           +IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G++YDEQ++YVVIKHAALFTST
Sbjct: 121 QIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTST 180

Query: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240
           IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV
Sbjct: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240

Query: 241 SSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVA 300
           SSCGHDGPFGATGVKRLKSIGMI+ VPGMKALDMN+AEDAIVRLTREVVPGMIVTGMEVA
Sbjct: 241 SSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVA 300

Query: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 352
           EIDGAPRMGPTFGAMMISGQKAAHLALKSLG  N ++         +LILAA +S E AD
Sbjct: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETAD 355

[Close]

BLAST of Carg00054 vs. ExPASy Swiss-Prot
Match: Q38709 (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 4.1e-150
Identity = 283/355 (79.72%), Postives = 311/355 (87.61%), Query Frame = 0


Query: 4   MASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASF--SISASASQQQPYDL 63
           MASTLT+K Q  +   +S   SSF+GTPLA P S+R++PT A    SIS S +   PYDL
Sbjct: 1   MASTLTSKPQRLALFENSA--SSFYGTPLA-PSSIRVQPTKAGAKPSISMSGAPSPPYDL 60

Query: 64  NSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAII 123
            +F F+PIKESIVSREMTRRYM DMITYADTDVV+VGAGS+GL C YELSKNP +++AII
Sbjct: 61  KAFTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAII 120

Query: 124 EQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKL 183
           EQSVSPGGGAWLGGQLFS MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKL 180

Query: 184 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 243
           LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 244 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 303
           DGPFGATGVK L+SIGMID+VPGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGA 300

Query: 304 PRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 352
           PRMGPTFGAMMISGQKAAHLALK+LGL N ++         +LILAA +S E+AD
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKALGLPNALDGSYVGGIHPELILAAADSAEIAD 351

[Close]

BLAST of Carg00054 vs. ExPASy Swiss-Prot
Match: F6H7K5 (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 4.5e-149
Identity = 286/359 (79.67%), Postives = 305/359 (84.96%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASF-SISASASQQQPY 60
           MASMA+TLT+    P  +      SSFHG+P+    S R+ P   S  S + S S   PY
Sbjct: 1   MASMATTLTSLSSNPKPAFFD-NKSSFHGSPI----SYRVLPIKVSHQSPTISMSSVSPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DL SFKF PIKESIV+REMTRRYM DMITYADTDVVIVGAGSAGLSCAYELSKNP IR+A
Sbjct: 61  DLQSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCI-------EDEAADLILAAGESPEVAD 352
           GAPRMGPTFGAMMISGQKAAHLAL++LG  N I       E    +LILAA E+ E+ D
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALRALGQPNAIDGNYTEAETMQPELILAAAETGEIVD 354

[Close]

BLAST of Carg00054 vs. ExPASy Swiss-Prot
Match: F6H9A9 (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 1.4e-147
Identity = 276/358 (77.09%), Postives = 312/358 (87.15%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLR-LKPTPASFSISASASQQQPY 60
           MA++ S++ +K   P +S      SSFHG P+A    L  +K TP + +++A+A    PY
Sbjct: 1   MATLTSSICSK---PKASVFDPHKSSFHGVPIATQARLSPVKSTPVNLAVTAAA---MPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DL SFKF PIKESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNP +++A
Sbjct: 61  DLRSFKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRL+S+GMIDSVPGMKALDMNTAED IVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIE-----DEAADLIL-AAGESPEVAD 352
           G+PRMGPTFGAMMISGQKAAHLALKSLGL N ++     +   +L+L AA ++ E+A+
Sbjct: 301 GSPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGTYIGNLHPELVLAAAADAAEIAE 352

[Close]

BLAST of Carg00054 vs. ExPASy Swiss-Prot
Match: Q38814 (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI1 PE=1 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 2.1e-146
Identity = 276/357 (77.31%), Postives = 308/357 (86.27%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPL-APPPSLRLKPTPASFSISASASQQQPY 60
           MA++ASTL+      S+    L DSSFHG+ + A P S+ LK  P SFS+ A+ +    Y
Sbjct: 1   MAAIASTLSLS----STKPQRLFDSSFHGSAISAAPISIGLK--PRSFSVRATTA---GY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLN+F F+PIKESIVSREMTRRYMTDMITYA+TDVV+VGAGSAGLS AYE+SKNP++++A
Sbjct: 61  DLNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQ+ YVV+KHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMID VPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIE-----DEAADLILAAGESPEVAD 352
           GAPRMGPTFGAMMISGQKA  LALK+LGL N I+     + + +L+LAA +S E  D
Sbjct: 301 GAPRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVD 348

[Close]

BLAST of Carg00054 vs. ExPASy TrEMBL
Match: A0A6J1JNT3 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487533 PE=3 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 6.6e-188
Identity = 347/355 (97.75%), Postives = 348/355 (98.03%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLA---PPPSLRLKPTPASFSISASASQQQ 60
           MASMAST TTKLQTPSSSSSSLLDSSFHGTPLA   PPPSLRLK TPA+FSISASASQ Q
Sbjct: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAAFSISASASQHQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGL NCIEDEAADLILAAGESPEVADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 355

[Close]

BLAST of Carg00054 vs. ExPASy TrEMBL
Match: A0A6J1FWL6 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448035 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.4e-184
Identity = 344/353 (97.45%), Postives = 346/353 (98.02%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLA-PPPSLRLKPTPASFSISASASQQQPY 60
           MAS+ASTLTTKLQTP   SSSLLDSSFHGTPLA PPPSLRLK TPA+FSI  SASQQQPY
Sbjct: 1   MASVASTLTTKLQTP---SSSLLDSSFHGTPLAPPPPSLRLKSTPAAFSI--SASQQQPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA
Sbjct: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 348

[Close]

BLAST of Carg00054 vs. ExPASy TrEMBL
Match: A0A0A0L546 (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.1e-166
Identity = 322/363 (88.71%), Postives = 329/363 (90.63%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPA-SFSISASASQQQPY 60
           MAS+ASTLTTKLQ P    S L DSSFHGTPLA P SLRLK T A S +ISASAS Q PY
Sbjct: 1   MASIASTLTTKLQKP----SLLHDSSFHGTPLASPSSLRLKSTAATSLTISASAS-QPPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLN FKFNPI+ESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNP+IRIA
Sbjct: 61  DLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPE 352
           GAPRMGPTFGAMMISGQKAAHLALKSLG AN I DE             +L+LAAGESPE
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPE 358

[Close]

BLAST of Carg00054 vs. ExPASy TrEMBL
Match: A0A1S3CJ81 (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.2e-166
Identity = 319/363 (87.88%), Postives = 328/363 (90.36%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLK-PTPASFSISASASQQQPY 60
           MAS+ASTLTTKLQ P    S L DSSFHGTPLA PPSLRLK P   S +ISASAS Q PY
Sbjct: 1   MASIASTLTTKLQIP----SLLHDSSFHGTPLASPPSLRLKSPAATSLTISASAS-QPPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLN FKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIA
Sbjct: 61  DLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPE 352
           GAPRMGPTFGAMMISGQKAAHLALKSLG AN I  E             +L+LAAGESPE
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPE 358

[Close]

BLAST of Carg00054 vs. ExPASy TrEMBL
Match: A0A6J1J7L4 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483293 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 5.1e-164
Identity = 308/361 (85.32%), Postives = 327/361 (90.58%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASFSISASAS-QQQPY 60
           MAS+AS+L TKL  P     SLLDSSFHG+PL P PS RLKPT A+ S+S SAS  Q PY
Sbjct: 1   MASIASSLPTKLHRP-----SLLDSSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLN FKFNPI+ESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSK+P +R+A
Sbjct: 61  DLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQEDYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIED---------EAADLILAAGESPEVA 352
           GAPRMGPTFGAMMISGQKAAHLALK+LG AN I++         EAA+++LAAGE+PEVA
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGRKVEAAEMVLAAGETPEVA 356

[Close]

BLAST of Carg00054 vs. TAIR 10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) )

HSP 1 Score: 520.0 bits (1338), Expect = 1.5e-147
Identity = 276/357 (77.31%), Postives = 308/357 (86.27%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPL-APPPSLRLKPTPASFSISASASQQQPY 60
           MA++ASTL+      S+    L DSSFHG+ + A P S+ LK  P SFS+ A+ +    Y
Sbjct: 1   MAAIASTLSLS----STKPQRLFDSSFHGSAISAAPISIGLK--PRSFSVRATTA---GY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLN+F F+PIKESIVSREMTRRYMTDMITYA+TDVV+VGAGSAGLS AYE+SKNP++++A
Sbjct: 61  DLNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQ+ YVV+KHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMID VPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIE-----DEAADLILAAGESPEVAD 352
           GAPRMGPTFGAMMISGQKA  LALK+LGL N I+     + + +L+LAA +S E  D
Sbjct: 301 GAPRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVD 348

The following BLAST results are available for this feature:

BLAST of Carg00054 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7031400.1	4.1e-192	100.00	Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyr...	[more]
KAG6600763.1	5.0e-190	99.43	Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]
XP_023524627.1	3.6e-188	98.87	thiamine thiazole synthase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022990741.1	1.4e-187	97.75	thiamine thiazole synthase, chloroplastic [Cucurbita maxima]	[more]
XP_022943248.1	7.0e-184	97.45	thiamine thiazole synthase, chloroplastic [Cucurbita moschata]	[more]

BLAST of Carg00054 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23787	1.1e-155	81.94	Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=...	[more]
Q38709	4.1e-150	79.72	Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=...	[more]
F6H7K5	4.5e-149	79.67	Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2...	[more]
F6H9A9	1.4e-147	77.09	Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1...	[more]
Q38814	2.1e-146	77.31	Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI...	[more]

BLAST of Carg00054 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JNT3	6.6e-188	97.75	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1FWL6	3.4e-184	97.45	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A0A0L546	1.1e-166	88.71	Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=...	[more]
A0A1S3CJ81	3.2e-166	87.88	Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160...	[more]
A0A6J1J7L4	5.1e-164	85.32	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of Carg00054 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54770.1	1.5e-147	77.31	thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	6.10.250.2840		coord: 60..90 e-value: 2.4E-19 score: 70.6
None	No IPR available	PFAM	PF01946	Thi4	coord: 75..308 e-value: 9.8E-115 score: 381.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..35
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	PANTHER	PTHR43422	THIAMINE THIAZOLE SYNTHASE	coord: 1..352
None	No IPR available	PANTHER	PTHR43422:SF11	THIAMINE THIAZOLE SYNTHASE, CHLOROPLASTIC	coord: 1..352
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 91..326 e-value: 1.6E-122 score: 409.3
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 58..320
IPR002922	Thiazole biosynthetic enzyme Thi4 family	TIGRFAM	TIGR00292	TIGR00292	coord: 71..326 e-value: 7.0E-82 score: 272.4
IPR027495	Thiamine thiazole synthase	HAMAP	MF_03158	THI4	coord: 5..335 score: 68.809822

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00054-RA	Carg00054-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0052837	thiazole biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005829	cytosol
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016763	pentosyltransferase activity