Carg00013 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00013
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionIdentified as a screen for stress-responsive genes.
LocationCarg_Chr04: 4883344 .. 4883850 (-)
RNA-Seq ExpressionCarg00013
SyntenyCarg00013
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGATACCTACTCCAAGATCGAAGCCGCCTGCAAATTGAAATCAAGATCTGTAGATTACTCCGATCTTTCATCGCTCCCTCACTCTCTTAGATTCGCCGCTGCCGACTCCAATCCGAACTCTCGCGATTCCGATCAAAACAGGACGAATCGGTCGCGGGAGCCGCCGCATGGTTGCTTGCCAGAGGAGGAAGAAGACGAATACCGCAGCGCTGCAACCGCTACTGCCTCTACGCTGAGACGGAACTGCTCTGTTTCTGCATCGGCCTCCGGATTCCACTCAGCAGTGAAGAGAGCTCTGTCGATGCGACGATCTTCGTCTGTGGCGGAGCGGTACAGTAGGATTCACGATCAGTTCGGGACGCTTGCATCGCCGATCGACGACGATGAAATCGAAGGCGAAGAATCGAAGGAAGGCCGAAATGCAGGAGGATCTGTAAGGAAGAAGAAGACGAACGCGGCTGGGAAGATCGTTAGAGCTTGTAAGCGACTTTTTGGACTGTAG

mRNA sequence

ATGGCCGATACCTACTCCAAGATCGAAGCCGCCTGCAAATTGAAATCAAGATCTGTAGATTACTCCGATCTTTCATCGCTCCCTCACTCTCTTAGATTCGCCGCTGCCGACTCCAATCCGAACTCTCGCGATTCCGATCAAAACAGGACGAATCGGTCGCGGGAGCCGCCGCATGGTTGCTTGCCAGAGGAGGAAGAAGACGAATACCGCAGCGCTGCAACCGCTACTGCCTCTACGCTGAGACGGAACTGCTCTGTTTCTGCATCGGCCTCCGGATTCCACTCAGCAGTGAAGAGAGCTCTGTCGATGCGACGATCTTCGTCTGTGGCGGAGCGGTACAGTAGGATTCACGATCAGTTCGGGACGCTTGCATCGCCGATCGACGACGATGAAATCGAAGGCGAAGAATCGAAGGAAGGCCGAAATGCAGGAGGATCTGTAAGGAAGAAGAAGACGAACGCGGCTGGGAAGATCGTTAGAGCTTGTAAGCGACTTTTTGGACTGTAG

Coding sequence (CDS)

ATGGCCGATACCTACTCCAAGATCGAAGCCGCCTGCAAATTGAAATCAAGATCTGTAGATTACTCCGATCTTTCATCGCTCCCTCACTCTCTTAGATTCGCCGCTGCCGACTCCAATCCGAACTCTCGCGATTCCGATCAAAACAGGACGAATCGGTCGCGGGAGCCGCCGCATGGTTGCTTGCCAGAGGAGGAAGAAGACGAATACCGCAGCGCTGCAACCGCTACTGCCTCTACGCTGAGACGGAACTGCTCTGTTTCTGCATCGGCCTCCGGATTCCACTCAGCAGTGAAGAGAGCTCTGTCGATGCGACGATCTTCGTCTGTGGCGGAGCGGTACAGTAGGATTCACGATCAGTTCGGGACGCTTGCATCGCCGATCGACGACGATGAAATCGAAGGCGAAGAATCGAAGGAAGGCCGAAATGCAGGAGGATCTGTAAGGAAGAAGAAGACGAACGCGGCTGGGAAGATCGTTAGAGCTTGTAAGCGACTTTTTGGACTGTAG

Protein sequence

MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRFAAADSNPNSRDSDQNRTNRSREPPHGCLPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHDQFGTLASPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRACKRLFGL
Homology
BLAST of Carg00013 vs. NCBI nr
Match: KAG7031359.1 (hypothetical protein SDJN02_05399, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 315.8 bits (808), Expect = 2.2e-82
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 0

Query: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRFAAADSNPNSRDSDQNRTNRSREPPHGC 60
           MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRFAAADSNPNSRDSDQNRTNRSREPPHGC
Sbjct: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRFAAADSNPNSRDSDQNRTNRSREPPHGC 60

Query: 61  LPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHDQF 120
           LPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHDQF
Sbjct: 61  LPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHDQF 120

Query: 121 GTLASPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRACKRLFGL 169
           GTLASPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRACKRLFGL
Sbjct: 121 GTLASPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRACKRLFGL 168

BLAST of Carg00013 vs. NCBI nr
Match: KAG6600720.1 (hypothetical protein SDJN03_05953, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 299.3 bits (765), Expect = 2.1e-77
Identity = 160/161 (99.38%), Postives = 160/161 (99.38%), Query Frame = 0

Query: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRFAAADSNPNSRDSDQNRTNRSREPPHGC 60
           MADTYSKIEAACKLKSRS DYSDLSSLPHSLRFAAADSNPNSRDSDQNRTNRSREPPHGC
Sbjct: 1   MADTYSKIEAACKLKSRSADYSDLSSLPHSLRFAAADSNPNSRDSDQNRTNRSREPPHGC 60

Query: 61  LPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHDQF 120
           LPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHDQF
Sbjct: 61  LPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHDQF 120

Query: 121 GTLASPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRA 162
           GTLASPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRA
Sbjct: 121 GTLASPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRA 161

BLAST of Carg00013 vs. NCBI nr
Match: XP_022136741.1 (uncharacterized protein LOC111008371 [Momordica charantia])

HSP 1 Score: 217.6 bits (553), Expect = 8.0e-53
Identity = 123/170 (72.35%), Postives = 135/170 (79.41%), Query Frame = 0

Query: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRFAAADSNPNS--RDSDQNRTNRSREPPH 60
           MAD YSKI+AACK KSRS+DYSDL+SLPHSL+F AA  NPNS   +SDQNR NR+R    
Sbjct: 1   MADGYSKIKAACKFKSRSIDYSDLASLPHSLKFTAAVPNPNSLAHESDQNRANRAR--AQ 60

Query: 61  GCLPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHD 120
            CLPEEEEDEY        + L RN SVS+SASG HSAVKRALSMRRSSSVAERY RIHD
Sbjct: 61  SCLPEEEEDEYGGGGGVAVAALSRNSSVSSSASGLHSAVKRALSMRRSSSVAERYCRIHD 120

Query: 121 QFGTLASPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRACKRLFGL 169
           QF TLASPIDD+EI   +SKE R + GSVR+KK NAAGKIVRACKRLFGL
Sbjct: 121 QFATLASPIDDEEIGAGDSKESRKSAGSVRRKKKNAAGKIVRACKRLFGL 168

BLAST of Carg00013 vs. NCBI nr
Match: KAG7015455.1 (hypothetical protein SDJN02_23091, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 209.5 bits (532), Expect = 2.2e-50
Identity = 125/176 (71.02%), Postives = 140/176 (79.55%), Query Frame = 0

Query: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRF--AAADSNPNSRDSDQNRTNR--SREP 60
           MAD+YSKI+AACK KSRS+DYSDL+SLPHS RF  AAA SNPNS DS++N+TNR  SR P
Sbjct: 1   MADSYSKIKAACKFKSRSIDYSDLTSLPHSPRFNAAAAVSNPNSHDSNKNKTNRQHSRLP 60

Query: 61  PHGCLPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRI 120
                 EEEEDEY    +A A+ L RN SVS+S SGFHSAVKRALSMRRSSSVAERY RI
Sbjct: 61  EEEEEEEEEEDEYGGEVSARAAALSRNNSVSSSVSGFHSAVKRALSMRRSSSVAERYCRI 120

Query: 121 HDQFGTLASPIDDDEIEGEESKEGRNAGGSV----RKKKTNAAGKIVRACKRLFGL 169
           HDQF TLASPIDDDE+E  +SKE R  GGSV    +KKK N+AGKIVRACKRLFG+
Sbjct: 121 HDQFATLASPIDDDEMEDGDSKEERKTGGSVKKTKKKKKKNSAGKIVRACKRLFGI 176

BLAST of Carg00013 vs. NCBI nr
Match: XP_022929198.1 (uncharacterized protein LOC111435863 [Cucurbita moschata] >KAG6577366.1 hypothetical protein SDJN03_24940, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 206.5 bits (524), Expect = 1.9e-49
Identity = 124/176 (70.45%), Postives = 139/176 (78.98%), Query Frame = 0

Query: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRF--AAADSNPNSRDSDQNRTNR--SREP 60
           MAD+YSKI+AACK KSRS+DYSDL+SLPHS RF  AAA SNPNS DS++N+TNR  SR P
Sbjct: 1   MADSYSKIKAACKFKSRSIDYSDLTSLPHSPRFNAAAAVSNPNSHDSNKNKTNRQHSRLP 60

Query: 61  PHGCLPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRI 120
                 EEEEDEY    +A A+ L RN SVS+S SGFHSAVKRALSMRRSSSVAERY RI
Sbjct: 61  EEEEEEEEEEDEYGGEVSARAAALSRNNSVSSSVSGFHSAVKRALSMRRSSSVAERYCRI 120

Query: 121 HDQFGTLASPIDDDEIEGEESKEGRNAGGSV----RKKKTNAAGKIVRACKRLFGL 169
           HDQF TLASPIDDDE+E  +SKE R  GGSV    +KKK N+A KIVRACKRLFG+
Sbjct: 121 HDQFATLASPIDDDEMEDGDSKEERKTGGSVKKTKKKKKKNSAEKIVRACKRLFGI 176

BLAST of Carg00013 vs. ExPASy TrEMBL
Match: A0A6J1C4C9 (uncharacterized protein LOC111008371 OS=Momordica charantia OX=3673 GN=LOC111008371 PE=4 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.9e-53
Identity = 123/170 (72.35%), Postives = 135/170 (79.41%), Query Frame = 0

Query: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRFAAADSNPNS--RDSDQNRTNRSREPPH 60
           MAD YSKI+AACK KSRS+DYSDL+SLPHSL+F AA  NPNS   +SDQNR NR+R    
Sbjct: 1   MADGYSKIKAACKFKSRSIDYSDLASLPHSLKFTAAVPNPNSLAHESDQNRANRAR--AQ 60

Query: 61  GCLPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHD 120
            CLPEEEEDEY        + L RN SVS+SASG HSAVKRALSMRRSSSVAERY RIHD
Sbjct: 61  SCLPEEEEDEYGGGGGVAVAALSRNSSVSSSASGLHSAVKRALSMRRSSSVAERYCRIHD 120

Query: 121 QFGTLASPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRACKRLFGL 169
           QF TLASPIDD+EI   +SKE R + GSVR+KK NAAGKIVRACKRLFGL
Sbjct: 121 QFATLASPIDDEEIGAGDSKESRKSAGSVRRKKKNAAGKIVRACKRLFGL 168

BLAST of Carg00013 vs. ExPASy TrEMBL
Match: A0A6J1ERE9 (uncharacterized protein LOC111435863 OS=Cucurbita moschata OX=3662 GN=LOC111435863 PE=4 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 9.0e-50
Identity = 124/176 (70.45%), Postives = 139/176 (78.98%), Query Frame = 0

Query: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRF--AAADSNPNSRDSDQNRTNR--SREP 60
           MAD+YSKI+AACK KSRS+DYSDL+SLPHS RF  AAA SNPNS DS++N+TNR  SR P
Sbjct: 1   MADSYSKIKAACKFKSRSIDYSDLTSLPHSPRFNAAAAVSNPNSHDSNKNKTNRQHSRLP 60

Query: 61  PHGCLPEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRI 120
                 EEEEDEY    +A A+ L RN SVS+S SGFHSAVKRALSMRRSSSVAERY RI
Sbjct: 61  EEEEEEEEEEDEYGGEVSARAAALSRNNSVSSSVSGFHSAVKRALSMRRSSSVAERYCRI 120

Query: 121 HDQFGTLASPIDDDEIEGEESKEGRNAGGSV----RKKKTNAAGKIVRACKRLFGL 169
           HDQF TLASPIDDDE+E  +SKE R  GGSV    +KKK N+A KIVRACKRLFG+
Sbjct: 121 HDQFATLASPIDDDEMEDGDSKEERKTGGSVKKTKKKKKKNSAEKIVRACKRLFGI 176

BLAST of Carg00013 vs. ExPASy TrEMBL
Match: A0A0A0L2N9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G110030 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.3e-45
Identity = 117/177 (66.10%), Postives = 131/177 (74.01%), Query Frame = 0

Query: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRFAAADSNPNSRDSDQNRTNRSREPPHGC 60
           MAD YSKI+AA K KSRS+DYSDLSSLPHSL F+AA SNP        RTNR +EPPHG 
Sbjct: 1   MADGYSKIKAASKFKSRSIDYSDLSSLPHSLTFSAAVSNP-------TRTNRPQEPPHGR 60

Query: 61  LPEEEED------EYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYS 120
           LPEE+E+      +    AT  A+TL RN SVS+S SGF SAVKRALSMRRSSSVAERY 
Sbjct: 61  LPEEDEEVNDDECDRPRGATTAAATLCRNSSVSSSVSGFQSAVKRALSMRRSSSVAERYC 120

Query: 121 RIHDQFGTLASPIDDDEIEGEESKEGRNAGGS---VRKKKTNAAGKIVRACKRLFGL 169
           RIHDQF T ASPI+DDE+EG + KE    GGS    +KKK NAA KIVRACKR+FGL
Sbjct: 121 RIHDQFATFASPIEDDEMEGGDWKERGKIGGSEIRKKKKKKNAAEKIVRACKRIFGL 170

BLAST of Carg00013 vs. ExPASy TrEMBL
Match: A0A5A7V402 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00790 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 4.3e-44
Identity = 117/171 (68.42%), Postives = 130/171 (76.02%), Query Frame = 0

Query: 1   MADTYSKIEAACKLKSRSVDYSDLSSLPHSLRF-AAADSNPNSRDSDQNRTNRSREPPHG 60
           MAD YSKI+AACK KSRS+DYSDLSSLPHSL F  AA SNP S  S+ +RTNR +EPPH 
Sbjct: 1   MADGYSKIKAACKFKSRSIDYSDLSSLPHSLTFNNAAVSNPISHHSNHSRTNRPQEPPHR 60

Query: 61  CLPEEEE----DEY-RSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYS 120
            LPEE+E    DE  R      A+TL RN SVS+S SGF SAVKRALSMRRSSSVAERY 
Sbjct: 61  RLPEEDEEVNDDECDRPRGGTAAATLSRNSSVSSSVSGFQSAVKRALSMRRSSSVAERYC 120

Query: 121 RIHDQFGTLASPIDDDEIEGEESKEGRNAGGSV--RKKKTNAAGKIVRACK 164
           RIHDQF T ASPI+DDE+EG +SKE    GGS   +KKKTNAAGKIVRA +
Sbjct: 121 RIHDQFATFASPIEDDELEGGDSKERGKLGGSAIKKKKKTNAAGKIVRALR 171

BLAST of Carg00013 vs. ExPASy TrEMBL
Match: A0A5N6QLE4 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_004482 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.2e-17
Identity = 76/164 (46.34%), Postives = 102/164 (62.20%), Query Frame = 0

Query: 5   YSKIEAACKLKSRSVDYSDLSSLPHSLRFAAADSNPNSRDSDQNRTNRSREPPHGCLPEE 64
           YSK++A    KSRS+D+SD  S   + +    +S+P S+  + ++   S          E
Sbjct: 4   YSKMKAIDTQKSRSMDFSDALSFSQTKK---TNSDPTSKPHEGSQIKESN--------AE 63

Query: 65  EEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSMRRSSSVAERYSRIHDQFGTLA 124
           +ED          + L R+CSVS S SGF SAVKRA S++RSSSV+ERY RI+DQ  TLA
Sbjct: 64  KEDGDGGNGEKFGAVLSRSCSVS-STSGFQSAVKRAFSVKRSSSVSERYCRIYDQSVTLA 123

Query: 125 SPIDDDEIEGEESKEGRNAGGSVRKKKTNAAGKIVRACKRLFGL 169
           SPIDDD+ EG ++   R    SV+KKK +  GKI++ACKRLFGL
Sbjct: 124 SPIDDDDDEGWDTM--RATRRSVKKKK-HKGGKILKACKRLFGL 152

BLAST of Carg00013 vs. TAIR 10
Match: AT4G24275.1 (Identified as a screen for stress-responsive genes. )

HSP 1 Score: 54.3 bits (129), Expect = 1.1e-07
Identity = 51/130 (39.23%), Postives = 72/130 (55.38%), Query Frame = 0

Query: 45  SDQNRTNRSREPPHGCL-PEEEEDEYRSAATATASTLRRNCSVSASASGFHSAVKRALSM 104
           S  + T++  EP H  + P+ ++ +   A       L RN SVSASA    S +K  +SM
Sbjct: 5   SKMDSTDQKLEPDHPVVDPQIQKPDLEPA--EMKPKLSRNRSVSASAQAVPSPIK--MSM 64

Query: 105 RRSSSVAERYSRIHDQFGTLASPI---DDDEIEGEESKEGRNAGGSVRKKKTNAAGK--I 164
           RRSSSV+ERY RI+DQ      P+   + DE E ++ KE  +     +KKK+N   K   
Sbjct: 65  RRSSSVSERYCRIYDQSSATTWPLPFHEGDEDEDDDDKEKVH-----KKKKSNNFKKKAF 124

Query: 165 VRACKRLFGL 169
           V+ACKR FG+
Sbjct: 125 VKACKRFFGI 125

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031359.12.2e-82100.00hypothetical protein SDJN02_05399, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6600720.12.1e-7799.38hypothetical protein SDJN03_05953, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022136741.18.0e-5372.35uncharacterized protein LOC111008371 [Momordica charantia][more]
KAG7015455.12.2e-5071.02hypothetical protein SDJN02_23091, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022929198.11.9e-4970.45uncharacterized protein LOC111435863 [Cucurbita moschata] >KAG6577366.1 hypothet... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1C4C93.9e-5372.35uncharacterized protein LOC111008371 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A6J1ERE99.0e-5070.45uncharacterized protein LOC111435863 OS=Cucurbita moschata OX=3662 GN=LOC1114358... [more]
A0A0A0L2N92.3e-4566.10Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G110030 PE=4 SV=1[more]
A0A5A7V4024.3e-4468.42Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5N6QLE41.2e-1746.34Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_004482 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G24275.11.1e-0739.23Identified as a screen for stress-responsive genes. [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..75
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 124..153
NoneNo IPR availablePANTHERPTHR38386:SF6OS05G0426900 PROTEINcoord: 3..168
NoneNo IPR availablePANTHERPTHR38386OS05G0426900 PROTEINcoord: 3..168

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00013-RACarg00013-RAmRNA