CaUC10G192620 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC10G192620
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
Descriptionacyl-CoA--sterol O-acyltransferase 1-like
LocationCiama_Chr10: 27236767 .. 27237831 (+)
RNA-Seq ExpressionCaUC10G192620
SyntenyCaUC10G192620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCGAGATTCATTCCCTTCTTCAAACTCTGTTCTTAGTTTCTTCATCTCTCTGTTTCTGTTTCATCATTAGAAACTCAATCCCCAATGGCTTCTTCAAATTCCTCTTAATTCTCCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCAAACCATCCATTTTCAAGGCCCCATCGGCTTCTTCATCGGCTGGCTTGGCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTGCTCCCCCGCCACCTCCTCCTCCCTCCTCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAAATCCCCGATCAATCAGCCGCAAATCACTCCAATCCCAAACCCGTTCTCTCCTTCAGCTACGGCGCAAAATTAGGGTTCCTAATTCTCACAATCGTCGCAATCAATTTCAAGAATCTCTTCCATCCAAACGCATCTCTACTTTTCTACTGCCTCTTAATTTACTTCTTCCTCGAATTTCTCATCGGCAGCTCCGCTGCCCTAGCCAAGGCGGTGCTCGGAGTCGAGTTATTGCCCTATTTCAACGAGCCCTATCTCTCTGATTCGTTACAGGATTTTTGGGGACGGCGATGGAATCTAATGACCTCTAGAATCCTCCGCCTCACCGTTTACGATCCGTGCAGGAAACTAACCAGCCGCGTAATCGGGAGGAAACCGGCGACGATGTTGGCGGTGATGGCGACATTTGTAGTCTCCGGTCTTATGCACGAACTGATCTACTTCTATATAATTAGATTGGCGCCGACTTGGGAGGTGACTTGCTTCTTTCTGGTACATGGAGTCTGTCTGGTGACGGAGATGGCAGTGAAGTTAGCAGTCGGCGGGAGGTTCCGGCTGCCGCAAGTTGTATCGACACCGTTGACGGTCGGGTTTGTGATGGGAACGGGTTGTTGGCTTTTTTTCCCACAGTGTTTGAGGGCTAAAATGGACGTCAGAATGTTTGAAGAGTACGCGGCCATTGGCGCATTTGTGAAGACTCTTACCGTCGATTTAATCACACCCTTCAATTTCATTTCGTTGCAATCCAAATAA

mRNA sequence

ATGGCCGCCGAGATTCATTCCCTTCTTCAAACTCTGTTCTTAGTTTCTTCATCTCTCTGTTTCTGTTTCATCATTAGAAACTCAATCCCCAATGGCTTCTTCAAATTCCTCTTAATTCTCCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCAAACCATCCATTTTCAAGGCCCCATCGGCTTCTTCATCGGCTGGCTTGGCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTGCTCCCCCGCCACCTCCTCCTCCCTCCTCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAAATCCCCGATCAATCAGCCGCAAATCACTCCAATCCCAAACCCGTTCTCTCCTTCAGCTACGGCGCAAAATTAGGGTTCCTAATTCTCACAATCGTCGCAATCAATTTCAAGAATCTCTTCCATCCAAACGCATCTCTACTTTTCTACTGCCTCTTAATTTACTTCTTCCTCGAATTTCTCATCGGCAGCTCCGCTGCCCTAGCCAAGGCGGTGCTCGGAGTCGAGTTATTGCCCTATTTCAACGAGCCCTATCTCTCTGATTCGTTACAGGATTTTTGGGGACGGCGATGGAATCTAATGACCTCTAGAATCCTCCGCCTCACCGTTTACGATCCGTGCAGGAAACTAACCAGCCGCGTAATCGGGAGGAAACCGGCGACGATGTTGGCGGTGATGGCGACATTTGTAGTCTCCGGTCTTATGCACGAACTGATCTACTTCTATATAATTAGATTGGCGCCGACTTGGGAGGTGACTTGCTTCTTTCTGGTACATGGAGTCTGTCTGGTGACGGAGATGGCAGTGAAGTTAGCAGTCGGCGGGAGGTTCCGGCTGCCGCAAGTTGTATCGACACCGTTGACGGTCGGGTTTGTGATGGGAACGGGTTGTTGGCTTTTTTTCCCACAGTGTTTGAGGGCTAAAATGGACGTCAGAATGTTTGAAGAGTACGCGGCCATTGGCGCATTTGTGAAGACTCTTACCGTCGATTTAATCACACCCTTCAATTTCATTTCGTTGCAATCCAAATAA

Coding sequence (CDS)

ATGGCCGCCGAGATTCATTCCCTTCTTCAAACTCTGTTCTTAGTTTCTTCATCTCTCTGTTTCTGTTTCATCATTAGAAACTCAATCCCCAATGGCTTCTTCAAATTCCTCTTAATTCTCCCCTTCTTCTTCCTCTTCCTCTACATTCCTCTCCAATTCCAAACCATCCATTTTCAAGGCCCCATCGGCTTCTTCATCGGCTGGCTTGGCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTGCTCCCCCGCCACCTCCTCCTCCCTCCTCCGCTTCCTCGCCGTCGGCTCCCTCCCAATCGAAATCCCCGATCAATCAGCCGCAAATCACTCCAATCCCAAACCCGTTCTCTCCTTCAGCTACGGCGCAAAATTAGGGTTCCTAATTCTCACAATCGTCGCAATCAATTTCAAGAATCTCTTCCATCCAAACGCATCTCTACTTTTCTACTGCCTCTTAATTTACTTCTTCCTCGAATTTCTCATCGGCAGCTCCGCTGCCCTAGCCAAGGCGGTGCTCGGAGTCGAGTTATTGCCCTATTTCAACGAGCCCTATCTCTCTGATTCGTTACAGGATTTTTGGGGACGGCGATGGAATCTAATGACCTCTAGAATCCTCCGCCTCACCGTTTACGATCCGTGCAGGAAACTAACCAGCCGCGTAATCGGGAGGAAACCGGCGACGATGTTGGCGGTGATGGCGACATTTGTAGTCTCCGGTCTTATGCACGAACTGATCTACTTCTATATAATTAGATTGGCGCCGACTTGGGAGGTGACTTGCTTCTTTCTGGTACATGGAGTCTGTCTGGTGACGGAGATGGCAGTGAAGTTAGCAGTCGGCGGGAGGTTCCGGCTGCCGCAAGTTGTATCGACACCGTTGACGGTCGGGTTTGTGATGGGAACGGGTTGTTGGCTTTTTTTCCCACAGTGTTTGAGGGCTAAAATGGACGTCAGAATGTTTGAAGAGTACGCGGCCATTGGCGCATTTGTGAAGACTCTTACCGTCGATTTAATCACACCCTTCAATTTCATTTCGTTGCAATCCAAATAA

Protein sequence

MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQGPIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPVLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGVELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMATFVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLTVGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFNFISLQSK
Homology
BLAST of CaUC10G192620 vs. NCBI nr
Match: XP_038903196.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 633.6 bits (1633), Expect = 9.9e-178
Identity = 326/355 (91.83%), Postives = 334/355 (94.08%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MAAEIHSLLQTLFLVSSSLCFCF IRNSIPNGF KFLLILPFFFLFLYIPLQFQTIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPD-QSAANHSNPKP 120
           PIGFFIGWLGSFKLLLFAFGKGPL S ATSSSL RFLAVGSLPIEIPD QS  NHSNPK 
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLSSAATSSSLPRFLAVGSLPIEIPDHQSPPNHSNPKL 120

Query: 121 VLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGV 180
           VL FSY AKL FLILT+VAINFKN FHPNA+LLFYCLLIYFFLEFLIGS+AA+AKAVLGV
Sbjct: 121 VLPFSYSAKLAFLILTMVAINFKNHFHPNAALLFYCLLIYFFLEFLIGSTAAVAKAVLGV 180

Query: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMAT 240
           ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLT  VIGRKPATMLAVMAT
Sbjct: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTIGVIGRKPATMLAVMAT 240

Query: 241 FVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLT 300
           FVVSGLMHELIYFYI RLAPTWEVTCFFLVHGVCLVTEMAVKLA GGRFR PQVVS PLT
Sbjct: 241 FVVSGLMHELIYFYIGRLAPTWEVTCFFLVHGVCLVTEMAVKLAAGGRFRPPQVVSIPLT 300

Query: 301 VGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFNFISLQSK 355
           +GFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVK++TVDLITPF F+SLQ K
Sbjct: 301 IGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKSITVDLITPFKFLSLQYK 355

BLAST of CaUC10G192620 vs. NCBI nr
Match: XP_004149743.1 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus])

HSP 1 Score: 544.7 bits (1402), Expect = 6.1e-151
Identity = 282/348 (81.03%), Postives = 302/348 (86.78%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MA+EIHSLLQTLFLVSSSL  CF IRNSIPNGFFKFLLILPFFFLFLYIPLQF TIHFQG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPD-QSAANHSNPKP 120
           PIGFFIGWLGSFKLLLFAFGKGPLCS ATSSSL RFLAVGSLPIEIPD +S  NHS   P
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120

Query: 121 VLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGV 180
                  AKL FLILTI+AINFKN  HPNA  LFYCLLIYFFLEFLIG++A LAK VLGV
Sbjct: 121 ------SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180

Query: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMAT 240
           ELLPYFNEPY SDSLQDFWGRRWNLMTSRILRLT+YDPCR LT  VIGR+PA+M+AV+AT
Sbjct: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIAT 240

Query: 241 FVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLT 300
           FVVSGLMHELIYFY+ R+APTWEVTCFF+VHGVC+V EMAV+L  GGRFR P+V    LT
Sbjct: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300

Query: 301 VGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFN 348
           + FVMGTG WLFFPQC+RAKMDVRM EEYAAIGAF KT+T+  I PFN
Sbjct: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFN 342

BLAST of CaUC10G192620 vs. NCBI nr
Match: XP_038905685.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 542.3 bits (1396), Expect = 3.0e-150
Identity = 276/348 (79.31%), Postives = 300/348 (86.21%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MAAEI   LQTLFLVSSSLCFCF IRNSIPNGF KFLLILPFFFLFLYIPLQFQ+IHFQG
Sbjct: 1   MAAEIRIFLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQSIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPD-QSAANHSNPKP 120
            IGFFIGWLGSFKLLLFAFGKGPLCS A SSSL  FLAVGSLPIEIPD + AANHS+  P
Sbjct: 61  SIGFFIGWLGSFKLLLFAFGKGPLCSAAASSSLRHFLAVGSLPIEIPDHKPAANHSHSNP 120

Query: 121 VLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGV 180
           VL  S+ +KLG LILTIVAI FKNLFHPNA L+F+C +IY  LE L+G +AALAKA LGV
Sbjct: 121 VLPLSFSSKLGLLILTIVAIYFKNLFHPNAILIFFCFVIYLLLEILLGGAAALAKATLGV 180

Query: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMAT 240
           ELLPYF+EPYLSDSLQDFWGRRWNLMTSRILRL VYDPCRK+T  ++GRKPA M+A+ AT
Sbjct: 181 ELLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLVVYDPCRKITIGIVGRKPAAMMAMTAT 240

Query: 241 FVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLT 300
           F VSGLMHELIYFY+ RLAPTWEVTCFFL+HGVCL  EMA+K AVGGRFRLP VVS  LT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSAVGGRFRLPGVVSAALT 300

Query: 301 VGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFN 348
           +GFVM T  WLFFPQ L+ K DVRMFEEYAA+GAFVK++T  LITP N
Sbjct: 301 IGFVMTTSFWLFFPQFLKLKSDVRMFEEYAALGAFVKSVTGQLITPLN 348

BLAST of CaUC10G192620 vs. NCBI nr
Match: XP_008461836.1 (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo] >KAA0048418.1 acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa] >TYJ97432.1 acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 537.7 bits (1384), Expect = 7.4e-149
Identity = 276/349 (79.08%), Postives = 301/349 (86.25%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MAAEIHSLLQTLFLVSSSL  CF IRNSIPNGF KFLL+LPFFFLFLYIPLQF TIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPV 120
           PIGFFIGWLGSFKLLLFAFG+GPLCS ATSSSL RFLAVGSLPIEIPD  +  H +  P 
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPHHSLLPP 120

Query: 121 LSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGVE 180
                 AKLGFLILTI+AINFKN FH NA LLFYCLLIYFFLEFLIG++AALAK VLGVE
Sbjct: 121 -----SAKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVE 180

Query: 181 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMATF 240
           LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCR LT  +IGR+ A+M+AVMATF
Sbjct: 181 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATF 240

Query: 241 VVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLTV 300
            VSGLMHELIYFY+ R+APTWEV+CFF+VHGVC+V E AVKL  GGR+R P+ V   LT+
Sbjct: 241 GVSGLMHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTI 300

Query: 301 GFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFNFI 350
           GFVMGTG WLFFPQC+RAKMDVRM EEYAA+GAF+K +TV  I   +F+
Sbjct: 301 GFVMGTGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFIDSISFL 344

BLAST of CaUC10G192620 vs. NCBI nr
Match: KAE8650923.1 (hypothetical protein Csa_001633 [Cucumis sativus])

HSP 1 Score: 523.9 bits (1348), Expect = 1.1e-144
Identity = 272/332 (81.93%), Postives = 290/332 (87.35%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MA+EIHSLLQTLFLVSSSL  CF IRNSIPNGFFKFLLILPFFFLFLYIPLQF TIHFQG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPD-QSAANHSNPKP 120
           PIGFFIGWLGSFKLLLFAFGKGPLCS ATSSSL RFLAVGSLPIEIPD +S  NHS   P
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120

Query: 121 VLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGV 180
                  AKL FLILTI+AINFKN  HPNA  LFYCLLIYFFLEFLIG++A LAK VLGV
Sbjct: 121 ------SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180

Query: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMAT 240
           ELLPYFNEPY SDSLQDFWGRRWNLMTSRILRLT+YDPCR LT  VIGR+PA+M+AV+AT
Sbjct: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIAT 240

Query: 241 FVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLT 300
           FVVSGLMHELIYFY+ R+APTWEVTCFF+VHGVC+V EMAV+L  GGRFR P+V    LT
Sbjct: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300

Query: 301 VGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAI 332
           + FVMGTG WLFFPQC+RAKMDVRM EEYAAI
Sbjct: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAI 326

BLAST of CaUC10G192620 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 4.3e-67
Identity = 147/338 (43.49%), Postives = 199/338 (58.88%), Query Frame = 0

Query: 7   SLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQGPIGFFI 66
           S ++   LV  SLC+ F I   +P G  + +L  P F +F  +P    ++H  G   FFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  GWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPVLSFS-- 126
            WL +FKLLLFA G+GPL S     SL  FLAV  LPI+I         +PKP  + S  
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI-------QLSPKPTKTHSHE 122

Query: 127 --------YGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAV 186
                   Y  K  F++L I A  +         L  Y + IYF LE ++ ++AA  +A+
Sbjct: 123 GSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAM 182

Query: 187 LGVELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAV 246
             +EL P FN+PYL+ SLQDFWGRRWNLM + ILR TVY+P  +L S V+G   + +LA 
Sbjct: 183 SDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAA 242

Query: 247 MATFVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVST 306
             TFVVSG+MHELI+FY+ RL P W++  FFL++G C   E+A+K  + GR+R P+ +S 
Sbjct: 243 FGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQ 302

Query: 307 PLTVGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAF 335
            LT+ FVM T  WLF P+  R  +  +  +EYAAIGAF
Sbjct: 303 VLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

BLAST of CaUC10G192620 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.1e-66
Identity = 148/348 (42.53%), Postives = 207/348 (59.48%), Query Frame = 0

Query: 4   EIHSLLQTLFLVSSSLCFCFIIRNSIPN-GFFKFLLILPFFFLFLYIPLQFQTIHFQGPI 63
           E+ +  +      ++ C+C  +    P+ G  + LL+LP   LF+++PL+  + H  GP 
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPVLS 123
             ++ WL +FKLLLFAF  GPL +P  S SLL F++   LPI+  D  + +H   K  LS
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPLSNP--SLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLS 125

Query: 124 FS------YGAKLGFLILTIVAINF-KNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKA 183
           F       + AKL F    +    F K+L H   S+L YC   Y   E  + +SA +A+A
Sbjct: 126 FEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVL-YCFHFYLGTEITLAASAVIARA 185

Query: 184 VLGVELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLA 243
            LG++L P FNEPYL+ SLQDFWGRRWNLM S IL LT Y P R++ SR +  +     A
Sbjct: 186 TLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGA 245

Query: 244 VMATFVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVS 303
           ++  F VSGLMHE+ +FY+ R  P+WEVT FF++HGVC   EM VK AV G+ RL + VS
Sbjct: 246 MLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVS 305

Query: 304 TPLTVGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLI 344
             LTVGFVM TG WLF PQ +R  +D++  +EY  +  + +   + L+
Sbjct: 306 GALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLL 350

BLAST of CaUC10G192620 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 6.7e-60
Identity = 138/337 (40.95%), Postives = 189/337 (56.08%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           M  E+ SL++       S+ +C+     I +G F+ L +LP   LFL +PL   ++HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPV 120
              FF+ WL +FKL+LF+F +GPL      S+L RF+     PI++       +  PK  
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLF--PVPSNLSRFVCFTCFPIKLQQNPKPQNQMPK-- 120

Query: 121 LSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGVE 180
             + +  KL F  + +    +K    P   L+ Y L IY   E L+     L    L  +
Sbjct: 121 --WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCD 180

Query: 181 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMATF 240
           L P+FNEPYLS SLQDFWGRRWNLM   ILR  VY P R++  R +    A  L V A+F
Sbjct: 181 LEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASF 240

Query: 241 VVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVK-LAVGGRFRLPQVVSTPLT 300
           +VSG++HELI+FY  R +PT EVT FF++HGVC   E A K   +  R+++ Q+VS  LT
Sbjct: 241 LVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLT 300

Query: 301 VGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVK 337
           VGFV+ TG WLFFP   R+ M  R+ +E      FVK
Sbjct: 301 VGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

BLAST of CaUC10G192620 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 4.2e-54
Identity = 133/343 (38.78%), Postives = 185/343 (53.94%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           M  +  +L++       SL +C+ I + +  G  + L ILP   LFL +PL    +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEI---PDQSAANHSNP 120
               F+ WL +FKLLLFAF +GPL        L RF+    LPI+I   P  +A  + +P
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLF--PLPPKLSRFICFACLPIKIRQDPSPNAIPNLHP 120

Query: 121 KPVLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVL 180
           KP+  +    K+  L + +    +++       L  YCL IY  +E ++    A+   +L
Sbjct: 121 KPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLL 180

Query: 181 GVELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVM 240
           G  + P FNEPYL+ SLQDFW RRWNLM S +LR TV+ P ++   R++    A    VM
Sbjct: 181 GCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVM 240

Query: 241 ATFVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLP-QVVST 300
           A+F VSGLMHEL+YFY+IR  PTWEVTCFF++HG    TE+AVK       R P + VS 
Sbjct: 241 ASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQW--LRPPHRAVSG 300

Query: 301 PLTVGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLT 340
            + + FV  TG WLF  Q LR  +  +   E   +    K  T
Sbjct: 301 LVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAKLFT 339

BLAST of CaUC10G192620 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 9.3e-54
Identity = 135/351 (38.46%), Postives = 197/351 (56.13%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           M  E+ +L++       S+ +C+ I + I  G  + L +LP F +FL +PL F ++HF  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPV 120
             GFF  WL +FKL LFAF + PL SP   S+L RF      PI+I    ++N  + KP+
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL-SP-LPSNLTRFFCFACFPIKINKNPSSNRIHNKPM 120

Query: 121 LSFSYGAKLGFLILTIVAINFKNLFHPNAS----LLFYCLLIYFFLEFLIGSSAALAKAV 180
             +    KL  LI + +   ++N +    S    L  + + +Y   E ++    AL   +
Sbjct: 121 SKWVLAFKL--LIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAV 240
           LG E+ P FN+PYL+ SLQ+FW RRWNLM   +LR  V+ P ++  + ++G   A    +
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 MATFVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFR-LP---Q 300
           +ATF+VSGLMHELIYFY+IR +PTWEVTCFFL+HGV    E+A+K     R R LP   +
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPRR 300

Query: 301 VVSTPLTVGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLI 344
            VS      F++ T  WLF+PQ LR  +  R+  E   +   VK   V ++
Sbjct: 301 AVSGLAITVFLLVTAGWLFYPQMLRNDVHKRVISECLLVIDVVKRHVVCIL 342

BLAST of CaUC10G192620 vs. ExPASy TrEMBL
Match: A0A0A0L9R8 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683670 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.9e-151
Identity = 282/348 (81.03%), Postives = 302/348 (86.78%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MA+EIHSLLQTLFLVSSSL  CF IRNSIPNGFFKFLLILPFFFLFLYIPLQF TIHFQG
Sbjct: 1   MASEIHSLLQTLFLVSSSLSLCFSIRNSIPNGFFKFLLILPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPD-QSAANHSNPKP 120
           PIGFFIGWLGSFKLLLFAFGKGPLCS ATSSSL RFLAVGSLPIEIPD +S  NHS   P
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPNHSLIPP 120

Query: 121 VLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGV 180
                  AKL FLILTI+AINFKN  HPNA  LFYCLLIYFFLEFLIG++A LAK VLGV
Sbjct: 121 ------SAKLIFLILTILAINFKNHLHPNAIPLFYCLLIYFFLEFLIGTTATLAKTVLGV 180

Query: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMAT 240
           ELLPYFNEPY SDSLQDFWGRRWNLMTSRILRLT+YDPCR LT  VIGR+PA+M+AV+AT
Sbjct: 181 ELLPYFNEPYFSDSLQDFWGRRWNLMTSRILRLTIYDPCRNLTIGVIGRRPASMVAVIAT 240

Query: 241 FVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLT 300
           FVVSGLMHELIYFY+ R+APTWEVTCFF+VHGVC+V EMAV+L  GGRFR P+V    LT
Sbjct: 241 FVVSGLMHELIYFYMGRMAPTWEVTCFFVVHGVCVVAEMAVRLGAGGRFRAPRVARICLT 300

Query: 301 VGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFN 348
           + FVMGTG WLFFPQC+RAKMDVRM EEYAAIGAF KT+T+  I PFN
Sbjct: 301 IMFVMGTGSWLFFPQCIRAKMDVRMLEEYAAIGAFFKTITLHFIPPFN 342

BLAST of CaUC10G192620 vs. ExPASy TrEMBL
Match: A0A5D3BC71 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold939G00050 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.6e-149
Identity = 276/349 (79.08%), Postives = 301/349 (86.25%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MAAEIHSLLQTLFLVSSSL  CF IRNSIPNGF KFLL+LPFFFLFLYIPLQF TIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPV 120
           PIGFFIGWLGSFKLLLFAFG+GPLCS ATSSSL RFLAVGSLPIEIPD  +  H +  P 
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPHHSLLPP 120

Query: 121 LSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGVE 180
                 AKLGFLILTI+AINFKN FH NA LLFYCLLIYFFLEFLIG++AALAK VLGVE
Sbjct: 121 -----SAKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVE 180

Query: 181 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMATF 240
           LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCR LT  +IGR+ A+M+AVMATF
Sbjct: 181 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATF 240

Query: 241 VVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLTV 300
            VSGLMHELIYFY+ R+APTWEV+CFF+VHGVC+V E AVKL  GGR+R P+ V   LT+
Sbjct: 241 GVSGLMHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTI 300

Query: 301 GFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFNFI 350
           GFVMGTG WLFFPQC+RAKMDVRM EEYAA+GAF+K +TV  I   +F+
Sbjct: 301 GFVMGTGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFIDSISFL 344

BLAST of CaUC10G192620 vs. ExPASy TrEMBL
Match: A0A1S3CG36 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500343 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.6e-149
Identity = 276/349 (79.08%), Postives = 301/349 (86.25%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MAAEIHSLLQTLFLVSSSL  CF IRNSIPNGF KFLL+LPFFFLFLYIPLQF TIHFQG
Sbjct: 1   MAAEIHSLLQTLFLVSSSLSICFSIRNSIPNGFLKFLLVLPFFFLFLYIPLQFHTIHFQG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPV 120
           PIGFFIGWLGSFKLLLFAFG+GPLCS ATSSSL RFLAVGSLPIEIPD  +  H +  P 
Sbjct: 61  PIGFFIGWLGSFKLLLFAFGRGPLCSAATSSSLPRFLAVGSLPIEIPDHKSPPHHSLLPP 120

Query: 121 LSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGVE 180
                 AKLGFLILTI+AINFKN FH NA LLFYCLLIYFFLEFLIG++AALAK VLGVE
Sbjct: 121 -----SAKLGFLILTILAINFKNHFHQNAVLLFYCLLIYFFLEFLIGTTAALAKKVLGVE 180

Query: 181 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMATF 240
           LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCR LT  +IGR+ A+M+AVMATF
Sbjct: 181 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRNLTVGIIGRRRASMVAVMATF 240

Query: 241 VVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLTV 300
            VSGLMHELIYFY+ R+APTWEV+CFF+VHGVC+V E AVKL  GGR+R P+ V   LT+
Sbjct: 241 GVSGLMHELIYFYMGRMAPTWEVSCFFVVHGVCVVAERAVKLGAGGRYRAPRAVRIGLTI 300

Query: 301 GFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFNFI 350
           GFVMGTG WLFFPQC+RAKMDVRM EEYAA+GAF+K +TV  I   +F+
Sbjct: 301 GFVMGTGSWLFFPQCIRAKMDVRMLEEYAAMGAFLKDITVLFIDSISFL 344

BLAST of CaUC10G192620 vs. ExPASy TrEMBL
Match: A0A6J1F144 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441426 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.2e-139
Identity = 258/355 (72.68%), Postives = 295/355 (83.10%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MAAEIH+ +QTLFL++SSLC CF IRNSIPNGF KFL +LPFFFLFLYIPLQFQTIH +G
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPD-QSAANHSNPKP 120
            +GFFIGWLGSFKLLLFAFGKGPLC  ATSSSL RFLAV  LPI+IP+ +S  N S+P P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 VLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGV 180
           +L  S+ AKLGFLILT  AI FKN F+PN  L+F+CLLIY  LE LIG + ALA+AVLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMAT 240
           ELLP+F+EPYLSDSLQDFWGRRWNL++SRILR  V+DPCRKL   V+G+KPA ++AVMAT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLT 300
           F VSG+MHELIYFY+ RL PTWEV+ FFL+HGVCL  EMAVK AVGGRFR P+VVS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 VGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFNFISLQSK 355
           +GFVM T  WLFFPQ LR K DVRM EEYAA+GAFVK++T +LI P  FIS Q K
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISFQVK 354

BLAST of CaUC10G192620 vs. ExPASy TrEMBL
Match: A0A6J1IX47 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481451 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.5e-139
Identity = 258/355 (72.68%), Postives = 295/355 (83.10%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           MAAEIH+ +QTLFL+SSSLC CF IRNSIPNGF KF+ +LPFFFLFLYIPLQFQTIH +G
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPD-QSAANHSNPKP 120
            +GFFIGWLGSFKLLLFAFGKGPLCS ATSSSL RFLAV SLPI+IP+ +   N S+P P
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 VLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGV 180
           +L  S+ AKLGFLILT  AI FKN F+PN  L+F+CLLIY  LE LIG + A+A+AVLGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 ELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMAT 240
           ELLP+F+EPYLSDSLQDFWGRRWNL++SRILR  V+DPCRKL   V+G+KPA ++AVMAT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVSTPLT 300
           F VSG+MHELIYFY+ RLAPTWEV+ FFL+HGVCL  EMAVK AVG RFR P+VVS  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 VGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLITPFNFISLQSK 355
           +GFVM T  WLFFPQ LR K DVRM EEYAA+GAFVK +T +LI P  FIS Q K
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGPL-FISFQVK 354

BLAST of CaUC10G192620 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 256.5 bits (654), Expect = 3.1e-68
Identity = 147/338 (43.49%), Postives = 199/338 (58.88%), Query Frame = 0

Query: 7   SLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQGPIGFFI 66
           S ++   LV  SLC+ F I   +P G  + +L  P F +F  +P    ++H  G   FFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  GWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPVLSFS-- 126
            WL +FKLLLFA G+GPL S     SL  FLAV  LPI+I         +PKP  + S  
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI-------QLSPKPTKTHSHE 122

Query: 127 --------YGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAV 186
                   Y  K  F++L I A  +         L  Y + IYF LE ++ ++AA  +A+
Sbjct: 123 GSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAM 182

Query: 187 LGVELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAV 246
             +EL P FN+PYL+ SLQDFWGRRWNLM + ILR TVY+P  +L S V+G   + +LA 
Sbjct: 183 SDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAA 242

Query: 247 MATFVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLPQVVST 306
             TFVVSG+MHELI+FY+ RL P W++  FFL++G C   E+A+K  + GR+R P+ +S 
Sbjct: 243 FGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQ 302

Query: 307 PLTVGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAF 335
            LT+ FVM T  WLF P+  R  +  +  +EYAAIGAF
Sbjct: 303 VLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

BLAST of CaUC10G192620 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 232.6 bits (592), Expect = 4.7e-61
Identity = 138/337 (40.95%), Postives = 189/337 (56.08%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           M  E+ SL++       S+ +C+     I +G F+ L +LP   LFL +PL   ++HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPV 120
              FF+ WL +FKL+LF+F +GPL      S+L RF+     PI++       +  PK  
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLF--PVPSNLSRFVCFTCFPIKLQQNPKPQNQMPK-- 120

Query: 121 LSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGVE 180
             + +  KL F  + +    +K    P   L+ Y L IY   E L+     L    L  +
Sbjct: 121 --WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCD 180

Query: 181 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMATF 240
           L P+FNEPYLS SLQDFWGRRWNLM   ILR  VY P R++  R +    A  L V A+F
Sbjct: 181 LEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASF 240

Query: 241 VVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVK-LAVGGRFRLPQVVSTPLT 300
           +VSG++HELI+FY  R +PT EVT FF++HGVC   E A K   +  R+++ Q+VS  LT
Sbjct: 241 LVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLT 300

Query: 301 VGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVK 337
           VGFV+ TG WLFFP   R+ M  R+ +E      FVK
Sbjct: 301 VGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

BLAST of CaUC10G192620 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 213.4 bits (542), Expect = 3.0e-55
Identity = 133/343 (38.78%), Postives = 185/343 (53.94%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           M  +  +L++       SL +C+ I + +  G  + L ILP   LFL +PL    +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEI---PDQSAANHSNP 120
               F+ WL +FKLLLFAF +GPL        L RF+    LPI+I   P  +A  + +P
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLF--PLPPKLSRFICFACLPIKIRQDPSPNAIPNLHP 120

Query: 121 KPVLSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVL 180
           KP+  +    K+  L + +    +++       L  YCL IY  +E ++    A+   +L
Sbjct: 121 KPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLL 180

Query: 181 GVELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVM 240
           G  + P FNEPYL+ SLQDFW RRWNLM S +LR TV+ P ++   R++    A    VM
Sbjct: 181 GCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVM 240

Query: 241 ATFVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFRLP-QVVST 300
           A+F VSGLMHEL+YFY+IR  PTWEVTCFF++HG    TE+AVK       R P + VS 
Sbjct: 241 ASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQW--LRPPHRAVSG 300

Query: 301 PLTVGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLT 340
            + + FV  TG WLF  Q LR  +  +   E   +    K  T
Sbjct: 301 LVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAKLFT 339

BLAST of CaUC10G192620 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 212.2 bits (539), Expect = 6.6e-55
Identity = 135/351 (38.46%), Postives = 197/351 (56.13%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           M  E+ +L++       S+ +C+ I + I  G  + L +LP F +FL +PL F ++HF  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPV 120
             GFF  WL +FKL LFAF + PL SP   S+L RF      PI+I    ++N  + KP+
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL-SP-LPSNLTRFFCFACFPIKINKNPSSNRIHNKPM 120

Query: 121 LSFSYGAKLGFLILTIVAINFKNLFHPNAS----LLFYCLLIYFFLEFLIGSSAALAKAV 180
             +    KL  LI + +   ++N +    S    L  + + +Y   E ++    AL   +
Sbjct: 121 SKWVLAFKL--LIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAV 240
           LG E+ P FN+PYL+ SLQ+FW RRWNLM   +LR  V+ P ++  + ++G   A    +
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 MATFVVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGRFR-LP---Q 300
           +ATF+VSGLMHELIYFY+IR +PTWEVTCFFL+HGV    E+A+K     R R LP   +
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPRR 300

Query: 301 VVSTPLTVGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKTLTVDLI 344
            VS      F++ T  WLF+PQ LR  +  R+  E   +   VK   V ++
Sbjct: 301 AVSGLAITVFLLVTAGWLFYPQMLRNDVHKRVISECLLVIDVVKRHVVCIL 342

BLAST of CaUC10G192620 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.5e-54
Identity = 135/338 (39.94%), Postives = 189/338 (55.92%), Query Frame = 0

Query: 1   MAAEIHSLLQTLFLVSSSLCFCFIIRNSIPNGFFKFLLILPFFFLFLYIPLQFQTIHFQG 60
           M  E+ + ++       S+ +C+ +   I  G F+ L +LP   LFL  PL F  +HF G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  PIGFFIGWLGSFKLLLFAFGKGPLCSPATSSSLLRFLAVGSLPIEIPDQSAANHSNPKPV 120
            + FF+ WL +FKL+LF+F +GPL SP    +L RF+ +   PI+ P Q+    +   P+
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL-SP-LPRTLSRFICITCFPIK-PQQNPNIQNYKIPI 120

Query: 121 LSFSYGAKLGFLILTIVAINFKNLFHPNASLLFYCLLIYFFLEFLIGSSAALAKAVLGVE 180
             F+   K+   ++ +    +K    P   L+F  L I+  LE +     AL    LG +
Sbjct: 121 WLFA--IKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCD 180

Query: 181 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTSRVIGRKPATMLAVMATF 240
           L P  NEPYL+ SLQDFWGRRWNLM   ILR  VY P R++  R +    A  L V A F
Sbjct: 181 LEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAF 240

Query: 241 VVSGLMHELIYFYIIRLAPTWEVTCFFLVHGVCLVTEMAVKLAVGGR--FRLPQVVSTPL 300
           +VSG +HE+++FY+ R  PT EVT FFL+HGVC V E+AVK +   R  +R+   VS  L
Sbjct: 241 LVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLL 300

Query: 301 TVGFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVK 337
           TVGFV+ T  W FFP  +R+ +  R+  E      FVK
Sbjct: 301 TVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903196.19.9e-17891.83acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida][more]
XP_004149743.16.1e-15181.03acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus][more]
XP_038905685.13.0e-15079.31acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida][more]
XP_008461836.17.4e-14979.08PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo] >KAA0048418.... [more]
KAE8650923.11.1e-14481.93hypothetical protein Csa_001633 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9SV074.3e-6743.49Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE... [more]
Q9XGY62.1e-6642.53Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ... [more]
Q9FJ756.7e-6040.95Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX... [more]
Q9FJ724.2e-5438.78Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX... [more]
Q9FJ739.3e-5438.46Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A0A0L9R82.9e-15181.03MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683670 PE=... [more]
A0A5D3BC713.6e-14979.08Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3CG363.6e-14979.08acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC10350034... [more]
A0A6J1F1441.2e-13972.68acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IX471.5e-13972.68acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G51970.13.1e-6843.49acyl-CoA sterol acyl transferase 1 [more]
AT5G55350.14.7e-6140.95MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55380.13.0e-5538.78MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55370.16.6e-5538.46MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55360.11.5e-5439.94MBOAT (membrane bound O-acyl transferase) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032805Wax synthase domainPFAMPF13813MBOAT_2coord: 183..268
e-value: 3.8E-17
score: 62.3
IPR017088Wax synthase, MagnoliopsidaPIRSFPIRSF037006Wax_synthasecoord: 1..350
e-value: 6.9E-113
score: 375.3
IPR044851Wax synthasePANTHERPTHR31595LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATEDcoord: 3..339
NoneNo IPR availablePANTHERPTHR31595:SF46ACYL-COA--STEROL O-ACYLTRANSFERASE 1coord: 3..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC10G192620.1CaUC10G192620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008374 O-acyltransferase activity