CaUC10G191590 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC10G191590
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionCytochrome P450
LocationCiama_Chr10: 25917844 .. 25918631 (+)
RNA-Seq ExpressionCaUC10G191590
SyntenyCaUC10G191590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAACTTCCAAAAGGTTCAATGGGTTTACTCATATTAGGTGAATCAAGGCAGTTCTTTGCTCCTAATCCATCTTTCGATTTATCTCCTTTCATCAAAGACAGAATTCTCAAGTACTTGATTTTTTTTCCACCTTTTTTGTATGTTAGGAAAAACCATCTATATTATTTTATTATAGTTTTTCTTAATTTAGTTATTTTTGTTTCAGGTATGGACCAGTTTTCAAAACAAGATTAGTGGGAAAACCATTAATTATCTCAGCAGATCATGAACTGAATTATATTATATTTCAACAAGAGGAACAATTGTTTGAGTGTTGGTATCCAGAGACCTTCAGAAAGATAATTGGAGGAAAATCTGTGGGGTCCTTGCATGGTTTTATGCATAAGTATTTGAAGAACACGATTATTTATGTTTTTGGAATTGAAATTTTGAAAAATATGATTTTTGTAAGTTGAAGTTGCAGCAACTACAAGATTGAAGAAATGGGCCTCTCATAACGTAGTTGAATTGAAGGATGAGATTGCAAATGTAAATTTATATTGAAAAAACTTGTTTTAATTATTTGAATACATATTATTCAATCAAGGTTTTAATTTTTCATTTATTAATATTAATTTTGTTCCATGGATACAGATGATATTTGATCTCACTGCAAAGAGATTAATTAGTTATGATCCAGAAAAGTCTTCAGAAAATTTGAGAGAAAACTTTGTTGCTTTCATTGAGGGTTTGATCTCTTTCCCTCTTCATATTCCTGGAACCTCCTTTCACAAATGCTTACAGCTG

mRNA sequence

TAAAACTTCCAAAAGGTTCAATGGGTTTACTCATATTAGGTGAATCAAGGCAGTTCTTTGCTCCTAATCCATCTTTCGATTTATCTCCTTTCATCAAAGACAGAATTCTCAAGTATGGACCAGTTTTCAAAACAAGATTAGTGGGAAAACCATTAATTATCTCAGCAGATCATGAACTGAATTATATTATATTTCAACAAGAGGAACAATTGTTTGAGTGTTGGTATCCAGAGACCTTCAGAAAGATAATTGGAGGAAAATCTGTGGGGTCCTTGCATGGTTTTATGCATAAGTATTTGAAGAACACGATTATTTATGTTTTTGGAATTGAAATTTTGAAAAATATGATTTTTGTAATTGCAGCAACTACAAGATTGAAGAAATGGGCCTCTCATAACGTAGTTGAATTGAAGGATGAGATTGCAAATATGATATTTGATCTCACTGCAAAGAGATTAATTAGTTATGATCCAGAAAAGTCTTCAGAAAATTTGAGAGAAAACTTTGTTGCTTTCATTGAGGGTTTGATCTCTTTCCCTCTTCATATTCCTGGAACCTCCTTTCACAAATGCTTACAGCTG

Coding sequence (CDS)

ATGGGTTTACTCATATTAGGTGAATCAAGGCAGTTCTTTGCTCCTAATCCATCTTTCGATTTATCTCCTTTCATCAAAGACAGAATTCTCAAGTATGGACCAGTTTTCAAAACAAGATTAGTGGGAAAACCATTAATTATCTCAGCAGATCATGAACTGAATTATATTATATTTCAACAAGAGGAACAATTGTTTGAGTGTTGGTATCCAGAGACCTTCAGAAAGATAATTGGAGGAAAATCTGTGGGGTCCTTGCATGGTTTTATGCATAAGTATTTGAAGAACACGATTATTTATGTTTTTGGAATTGAAATTTTGAAAAATATGATTTTTGTAATTGCAGCAACTACAAGATTGAAGAAATGGGCCTCTCATAACGTAGTTGAATTGAAGGATGAGATTGCAAATATGATATTTGATCTCACTGCAAAGAGATTAATTAGTTATGATCCAGAAAAGTCTTCAGAAAATTTGAGAGAAAACTTTGTTGCTTTCATTGAGGGTTTGATCTCTTTCCCTCTTCATATTCCTGGAACCTCCTTTCACAAATGCTTACAGCTG

Protein sequence

MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQEEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVIAATTRLKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIPGTSFHKCLQL
Homology
BLAST of CaUC10G191590 vs. NCBI nr
Match: XP_004141154.1 (cytochrome P450 87A3 isoform X1 [Cucumis sativus])

HSP 1 Score: 317.4 bits (812), Expect = 8.3e-83
Identity = 155/189 (82.01%), Postives = 168/189 (88.89%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG   +GES QFFAPNPSFDLSPFIK RILKYGP+FKTRLVGKPLIISAD ELN+IIFQ+
Sbjct: 34  MGFPFVGESTQFFAPNPSFDLSPFIKHRILKYGPIFKTRLVGKPLIISADAELNHIIFQK 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMI--FVIAATTR 120
           EE+LFECWYPETFRKI G KSVGSLHGFMHKYLKN I  VFGIE LKNMI    + +TTR
Sbjct: 94  EEELFECWYPETFRKIFGVKSVGSLHGFMHKYLKNMITNVFGIESLKNMISEVEVTSTTR 153

Query: 121 LKKWASHN-VVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIP 180
           LKKWASHN +VELKDEIANMIFDL+AKRLISYDPEK  EN+RENFVAFI+GLISFP+ IP
Sbjct: 154 LKKWASHNDIVELKDEIANMIFDLSAKRLISYDPEKCGENMRENFVAFIQGLISFPIDIP 213

Query: 181 GTSFHKCLQ 187
           GTS+HKCLQ
Sbjct: 214 GTSYHKCLQ 222

BLAST of CaUC10G191590 vs. NCBI nr
Match: XP_031737523.1 (cytochrome P450 87A3 isoform X2 [Cucumis sativus])

HSP 1 Score: 317.4 bits (812), Expect = 8.3e-83
Identity = 155/189 (82.01%), Postives = 168/189 (88.89%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG   +GES QFFAPNPSFDLSPFIK RILKYGP+FKTRLVGKPLIISAD ELN+IIFQ+
Sbjct: 34  MGFPFVGESTQFFAPNPSFDLSPFIKHRILKYGPIFKTRLVGKPLIISADAELNHIIFQK 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMI--FVIAATTR 120
           EE+LFECWYPETFRKI G KSVGSLHGFMHKYLKN I  VFGIE LKNMI    + +TTR
Sbjct: 94  EEELFECWYPETFRKIFGVKSVGSLHGFMHKYLKNMITNVFGIESLKNMISEVEVTSTTR 153

Query: 121 LKKWASHN-VVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIP 180
           LKKWASHN +VELKDEIANMIFDL+AKRLISYDPEK  EN+RENFVAFI+GLISFP+ IP
Sbjct: 154 LKKWASHNDIVELKDEIANMIFDLSAKRLISYDPEKCGENMRENFVAFIQGLISFPIDIP 213

Query: 181 GTSFHKCLQ 187
           GTS+HKCLQ
Sbjct: 214 GTSYHKCLQ 222

BLAST of CaUC10G191590 vs. NCBI nr
Match: XP_022935245.1 (cytochrome P450 87A3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 297.0 bits (759), Expect = 1.2e-76
Identity = 139/188 (73.94%), Postives = 161/188 (85.64%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG  ILGE+  FFAPNPSFD+SPFIKDR+LKYGP+FKT LVGKPLIIS D +LNY+IF+Q
Sbjct: 34  MGFPILGETNHFFAPNPSFDVSPFIKDRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVI--AATTR 120
           EE LFECWYPETF+KI G +  GSLHGFMHKYLKN I+  FGIE LK+M+  +  AATTR
Sbjct: 94  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSMVSEVEAAATTR 153

Query: 121 LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIPG 180
           L++WAS  VVELKDEIAN+I DLTA +LISYDPE S ENLRENFVAFI+GL+SFPL IPG
Sbjct: 154 LRRWASCKVVELKDEIANVILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 213

Query: 181 TSFHKCLQ 187
           T++HKCL+
Sbjct: 214 TAYHKCLR 221

BLAST of CaUC10G191590 vs. NCBI nr
Match: XP_022935244.1 (cytochrome P450 87A3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 297.0 bits (759), Expect = 1.2e-76
Identity = 139/188 (73.94%), Postives = 161/188 (85.64%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG  ILGE+  FFAPNPSFD+SPFIKDR+LKYGP+FKT LVGKPLIIS D +LNY+IF+Q
Sbjct: 34  MGFPILGETNHFFAPNPSFDVSPFIKDRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVI--AATTR 120
           EE LFECWYPETF+KI G +  GSLHGFMHKYLKN I+  FGIE LK+M+  +  AATTR
Sbjct: 94  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSMVSEVEAAATTR 153

Query: 121 LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIPG 180
           L++WAS  VVELKDEIAN+I DLTA +LISYDPE S ENLRENFVAFI+GL+SFPL IPG
Sbjct: 154 LRRWASCKVVELKDEIANVILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 213

Query: 181 TSFHKCLQ 187
           T++HKCL+
Sbjct: 214 TAYHKCLR 221

BLAST of CaUC10G191590 vs. NCBI nr
Match: XP_022983296.1 (cytochrome P450 87A3-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 295.0 bits (754), Expect = 4.4e-76
Identity = 138/188 (73.40%), Postives = 159/188 (84.57%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG  ILGE+  FFAPNPSFD+SPFIK R+ KYGP+FKT LVGKPLIIS D +LNY+IF+Q
Sbjct: 34  MGFPILGETNHFFAPNPSFDVSPFIKHRVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVI--AATTR 120
           EE LFECWYPETF+KI G +  GSLHGFMHKYLKN I+  FGIE LK+M+F +  AA TR
Sbjct: 94  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSMVFEVEAAAITR 153

Query: 121 LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIPG 180
           L++WAS  VVELKDEIANMI DLTA +LISYDPE S ENLRENFVAFI+GL+SFPL IPG
Sbjct: 154 LRRWASCEVVELKDEIANMILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 213

Query: 181 TSFHKCLQ 187
           T++HKCL+
Sbjct: 214 TAYHKCLR 221

BLAST of CaUC10G191590 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 234.6 bits (597), Expect = 9.3e-61
Identity = 109/190 (57.37%), Postives = 148/190 (77.89%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           +GL ++GE+ QFFAPNP+ DLSPF+K+RI +YG +FKT +VG+P+++SAD E+NY +FQQ
Sbjct: 76  LGLPVIGETLQFFAPNPTCDLSPFVKERIKRYGSIFKTSVVGRPVVVSADPEMNYYVFQQ 135

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFV---IAATT 120
           E +LFE WYP+TF +I G  +VGSLHGFM+KYLK  ++ ++G E LK+++      A   
Sbjct: 136 EGKLFESWYPDTFTEIFGRDNVGSLHGFMYKYLKTLVLRLYGQENLKSVLLAETDAACRG 195

Query: 121 RLKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSE-NLRENFVAFIEGLISFPLHI 180
            L  WAS   VELK+ I+ MIFDLTAK+LI YDP K S+ NLR+NF AFI GLISFPL+I
Sbjct: 196 SLASWASQPSVELKEGISTMIFDLTAKKLIGYDPSKPSQVNLRKNFGAFICGLISFPLNI 255

Query: 181 PGTSFHKCLQ 187
           PGT++H+C++
Sbjct: 256 PGTAYHECME 265

BLAST of CaUC10G191590 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 6.9e-32
Identity = 69/190 (36.32%), Postives = 112/190 (58.95%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MGL ++GE+ Q   P+ S D+ PFI+ ++ +YGP+FKT L G+P+++SAD E N  I  Q
Sbjct: 37  MGLPLIGETIQLSRPSDSLDVHPFIQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQ 96

Query: 61  EEQLFECWYPETFRKIIGGKSVG-SLHGFMHKYLKNTIIYVFGIEILKN--MIFVIAATT 120
           E +  E WY +T  K  G  +      G +HKY+++  +  FG E L+   + F+ A++ 
Sbjct: 97  EGRAVEMWYLDTLSKFFGLDTEWLKALGLIHKYIRSITLNHFGAEALRERFLPFIEASSM 156

Query: 121 R-LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHI 180
             L  W++   VE+K+  A M+F  +  ++   D +K S N+   F   + G +S PL+ 
Sbjct: 157 EALHSWSTQPSVEVKNASALMVFRTSVNKMFGEDAKKLSGNIPGKFTKLLGGFLSLPLNF 216

Query: 181 PGTSFHKCLQ 187
           PGT++HKCL+
Sbjct: 217 PGTTYHKCLK 226

BLAST of CaUC10G191590 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 1.8e-27
Identity = 63/189 (33.33%), Postives = 107/189 (56.61%), Query Frame = 0

Query: 2   GLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQE 61
           G   LGE+  +  P  +  L  F++  + KYG ++++ L G+P I+SAD  LN  I Q E
Sbjct: 45  GWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNRFILQNE 104

Query: 62  EQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVIAATTR--- 121
            +LFEC YP +   I+G  S+  L G MH+ +++  +       L+ ++           
Sbjct: 105 GRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVERHTLFV 164

Query: 122 LKKWASHNVVELKDEIANMIFDLTAKRLISYDP-EKSSENLRENFVAFIEGLISFPLHIP 181
           L  W  +++   +DE     F+L AK ++S DP E+ +E L++ +V F++G++S PL++P
Sbjct: 165 LDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLP 224

Query: 182 GTSFHKCLQ 187
           GT++HK LQ
Sbjct: 225 GTAYHKALQ 233

BLAST of CaUC10G191590 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.3e-27
Identity = 67/189 (35.45%), Postives = 108/189 (57.14%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           +GL ++GE+ Q      + +  PFI +R+ +YG VF T L G+P I SAD E N  + Q 
Sbjct: 36  LGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNRFVLQN 95

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFV-IAATTR- 120
           E +LFEC YP +   ++G  S+  + G +HK + +  +      I+K+ + + I    R 
Sbjct: 96  EGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRF 155

Query: 121 -LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIP 180
            L  W+S   V L +E   + F+LT K+L+S+DP + SE+LR+ ++  IEG  S PL + 
Sbjct: 156 NLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLF 215

Query: 181 GTSFHKCLQ 187
            T++ K +Q
Sbjct: 216 STTYRKAIQ 222

BLAST of CaUC10G191590 vs. ExPASy Swiss-Prot
Match: Q6F4F5 (Cytochrome P450 724B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP724B1 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.7e-26
Identity = 69/198 (34.85%), Postives = 109/198 (55.05%), Query Frame = 0

Query: 2   GLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQE 61
           G  +LGE+ +F +P+ S  L  F++D   +YG VFK+ L   P I+S D ELN+ I Q E
Sbjct: 41  GWPLLGETLRFLSPHASNTLGSFLEDHCSRYGRVFKSHLFCTPTIVSCDQELNHFILQNE 100

Query: 62  EQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTII-----------YVFGIEILKNMI 121
           E+LF+C YP     I+G  S+  + G  HK L+N  +           Y+  IE  K  +
Sbjct: 101 ERLFQCSYPRPIHGILGKSSMLVVLGEDHKRLRNLALALVTSTKLKPSYLGDIE--KIAL 160

Query: 122 FVIAA-TTRLKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKS-SENLRENFVAFIEG 181
            ++ +   + K     NV+   +E     F +  K+++   PE+  +  + E+F+AF++G
Sbjct: 161 HIVGSWHGKSKDKGMVNVIAFCEEARKFAFSVIVKQVLGLSPEEPVTAMILEDFLAFMKG 220

Query: 182 LISFPLHIPGTSFHKCLQ 187
           LISFPL+IPGT + K +Q
Sbjct: 221 LISFPLYIPGTPYAKAVQ 236

BLAST of CaUC10G191590 vs. ExPASy TrEMBL
Match: A0A0A0LCN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G845470 PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 4.0e-83
Identity = 155/189 (82.01%), Postives = 168/189 (88.89%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG   +GES QFFAPNPSFDLSPFIK RILKYGP+FKTRLVGKPLIISAD ELN+IIFQ+
Sbjct: 34  MGFPFVGESTQFFAPNPSFDLSPFIKHRILKYGPIFKTRLVGKPLIISADAELNHIIFQK 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMI--FVIAATTR 120
           EE+LFECWYPETFRKI G KSVGSLHGFMHKYLKN I  VFGIE LKNMI    + +TTR
Sbjct: 94  EEELFECWYPETFRKIFGVKSVGSLHGFMHKYLKNMITNVFGIESLKNMISEVEVTSTTR 153

Query: 121 LKKWASHN-VVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIP 180
           LKKWASHN +VELKDEIANMIFDL+AKRLISYDPEK  EN+RENFVAFI+GLISFP+ IP
Sbjct: 154 LKKWASHNDIVELKDEIANMIFDLSAKRLISYDPEKCGENMRENFVAFIQGLISFPIDIP 213

Query: 181 GTSFHKCLQ 187
           GTS+HKCLQ
Sbjct: 214 GTSYHKCLQ 222

BLAST of CaUC10G191590 vs. ExPASy TrEMBL
Match: A0A6J1FA36 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442183 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.6e-77
Identity = 139/188 (73.94%), Postives = 161/188 (85.64%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG  ILGE+  FFAPNPSFD+SPFIKDR+LKYGP+FKT LVGKPLIIS D +LNY+IF+Q
Sbjct: 34  MGFPILGETNHFFAPNPSFDVSPFIKDRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVI--AATTR 120
           EE LFECWYPETF+KI G +  GSLHGFMHKYLKN I+  FGIE LK+M+  +  AATTR
Sbjct: 94  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSMVSEVEAAATTR 153

Query: 121 LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIPG 180
           L++WAS  VVELKDEIAN+I DLTA +LISYDPE S ENLRENFVAFI+GL+SFPL IPG
Sbjct: 154 LRRWASCKVVELKDEIANVILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 213

Query: 181 TSFHKCLQ 187
           T++HKCL+
Sbjct: 214 TAYHKCLR 221

BLAST of CaUC10G191590 vs. ExPASy TrEMBL
Match: A0A6J1F4V6 (cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442183 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.6e-77
Identity = 139/188 (73.94%), Postives = 161/188 (85.64%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG  ILGE+  FFAPNPSFD+SPFIKDR+LKYGP+FKT LVGKPLIIS D +LNY+IF+Q
Sbjct: 34  MGFPILGETNHFFAPNPSFDVSPFIKDRVLKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVI--AATTR 120
           EE LFECWYPETF+KI G +  GSLHGFMHKYLKN I+  FGIE LK+M+  +  AATTR
Sbjct: 94  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSMVSEVEAAATTR 153

Query: 121 LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIPG 180
           L++WAS  VVELKDEIAN+I DLTA +LISYDPE S ENLRENFVAFI+GL+SFPL IPG
Sbjct: 154 LRRWASCKVVELKDEIANVILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 213

Query: 181 TSFHKCLQ 187
           T++HKCL+
Sbjct: 214 TAYHKCLR 221

BLAST of CaUC10G191590 vs. ExPASy TrEMBL
Match: A0A6J1J5G8 (cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481917 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.1e-76
Identity = 138/188 (73.40%), Postives = 159/188 (84.57%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG  ILGE+  FFAPNPSFD+SPFIK R+ KYGP+FKT LVGKPLIIS D +LNY+IF+Q
Sbjct: 34  MGFPILGETNHFFAPNPSFDVSPFIKHRVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVI--AATTR 120
           EE LFECWYPETF+KI G +  GSLHGFMHKYLKN I+  FGIE LK+M+F +  AA TR
Sbjct: 94  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSMVFEVEAAAITR 153

Query: 121 LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIPG 180
           L++WAS  VVELKDEIANMI DLTA +LISYDPE S ENLRENFVAFI+GL+SFPL IPG
Sbjct: 154 LRRWASCEVVELKDEIANMILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 213

Query: 181 TSFHKCLQ 187
           T++HKCL+
Sbjct: 214 TAYHKCLR 221

BLAST of CaUC10G191590 vs. ExPASy TrEMBL
Match: A0A6J1J1Q7 (cytochrome P450 87A3-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481917 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.1e-76
Identity = 138/188 (73.40%), Postives = 159/188 (84.57%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG  ILGE+  FFAPNPSFD+SPFIK R+ KYGP+FKT LVGKPLIIS D +LNY+IF+Q
Sbjct: 34  MGFPILGETNHFFAPNPSFDVSPFIKHRVFKYGPIFKTSLVGKPLIISTDPDLNYLIFEQ 93

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVI--AATTR 120
           EE LFECWYPETF+KI G +  GSLHGFMHKYLKN I+  FGIE LK+M+F +  AA TR
Sbjct: 94  EETLFECWYPETFKKIFGDQFFGSLHGFMHKYLKNMIVNAFGIECLKSMVFEVEAAAITR 153

Query: 121 LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIPG 180
           L++WAS  VVELKDEIANMI DLTA +LISYDPE S ENLRENFVAFI+GL+SFPL IPG
Sbjct: 154 LRRWASCEVVELKDEIANMILDLTANKLISYDPENSPENLRENFVAFIQGLVSFPLDIPG 213

Query: 181 TSFHKCLQ 187
           T++HKCL+
Sbjct: 214 TAYHKCLR 221

BLAST of CaUC10G191590 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 246.9 bits (629), Expect = 1.3e-65
Identity = 114/188 (60.64%), Postives = 148/188 (78.72%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           MG  +LGES QFF PN + D+ PFIK+R+ KYGP+FKT LVG+P+I+S D +L+Y +F Q
Sbjct: 36  MGFPLLGESIQFFKPNKTSDIPPFIKERVKKYGPIFKTNLVGRPVIVSTDADLSYFVFNQ 95

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMI--FVIAATTR 120
           E + F+ WYP+TF  I G K+VGSLHGFM+KYLKN ++ +FG + LK M+    + A  R
Sbjct: 96  EGRCFQSWYPDTFTHIFGKKNVGSLHGFMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKR 155

Query: 121 LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIPG 180
           L+ W++ + VELKD  A+MIFDLTAK+LIS+DP+KSSENLR NFVAFI+GLISFP  IPG
Sbjct: 156 LELWSNQDSVELKDATASMIFDLTAKKLISHDPDKSSENLRANFVAFIQGLISFPFDIPG 215

Query: 181 TSFHKCLQ 187
           T++HKCLQ
Sbjct: 216 TAYHKCLQ 223

BLAST of CaUC10G191590 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 239.6 bits (610), Expect = 2.1e-63
Identity = 113/194 (58.25%), Postives = 148/194 (76.29%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRI------LKYGPVFKTRLVGKPLIISADHELN 60
           MG  +LGES QFF PN + D+ PFIK+R+       +YGP+FKT LVG+P+I+S D +L+
Sbjct: 36  MGFPLLGESIQFFKPNKTSDIPPFIKERVKNDVDMCRYGPIFKTNLVGRPVIVSTDADLS 95

Query: 61  YIIFQQEEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMI--FV 120
           Y +F QE + F+ WYP+TF  I G K+VGSLHGFM+KYLKN ++ +FG + LK M+    
Sbjct: 96  YFVFNQEGRCFQSWYPDTFTHIFGKKNVGSLHGFMYKYLKNMVLTLFGHDGLKKMLPQVE 155

Query: 121 IAATTRLKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISF 180
           + A  RL+ W++ + VELKD  A+MIFDLTAK+LIS+DP+KSSENLR NFVAFI+GLISF
Sbjct: 156 MTANKRLELWSNQDSVELKDATASMIFDLTAKKLISHDPDKSSENLRANFVAFIQGLISF 215

Query: 181 PLHIPGTSFHKCLQ 187
           P  IPGT++HKCLQ
Sbjct: 216 PFDIPGTAYHKCLQ 229

BLAST of CaUC10G191590 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 124.0 bits (310), Expect = 1.3e-28
Identity = 63/189 (33.33%), Postives = 107/189 (56.61%), Query Frame = 0

Query: 2   GLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQE 61
           G   LGE+  +  P  +  L  F++  + KYG ++++ L G+P I+SAD  LN  I Q E
Sbjct: 45  GWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNRFILQNE 104

Query: 62  EQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFVIAATTR--- 121
            +LFEC YP +   I+G  S+  L G MH+ +++  +       L+ ++           
Sbjct: 105 GRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVERHTLFV 164

Query: 122 LKKWASHNVVELKDEIANMIFDLTAKRLISYDP-EKSSENLRENFVAFIEGLISFPLHIP 181
           L  W  +++   +DE     F+L AK ++S DP E+ +E L++ +V F++G++S PL++P
Sbjct: 165 LDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLP 224

Query: 182 GTSFHKCLQ 187
           GT++HK LQ
Sbjct: 225 GTAYHKALQ 233

BLAST of CaUC10G191590 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-28
Identity = 67/189 (35.45%), Postives = 108/189 (57.14%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           +GL ++GE+ Q      + +  PFI +R+ +YG VF T L G+P I SAD E N  + Q 
Sbjct: 36  LGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNRFVLQN 95

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFV-IAATTR- 120
           E +LFEC YP +   ++G  S+  + G +HK + +  +      I+K+ + + I    R 
Sbjct: 96  EGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRF 155

Query: 121 -LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIP 180
            L  W+S   V L +E   + F+LT K+L+S+DP + SE+LR+ ++  IEG  S PL + 
Sbjct: 156 NLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLF 215

Query: 181 GTSFHKCLQ 187
            T++ K +Q
Sbjct: 216 STTYRKAIQ 222

BLAST of CaUC10G191590 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-28
Identity = 67/189 (35.45%), Postives = 108/189 (57.14%), Query Frame = 0

Query: 1   MGLLILGESRQFFAPNPSFDLSPFIKDRILKYGPVFKTRLVGKPLIISADHELNYIIFQQ 60
           +GL ++GE+ Q      + +  PFI +R+ +YG VF T L G+P I SAD E N  + Q 
Sbjct: 36  LGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNRFVLQN 95

Query: 61  EEQLFECWYPETFRKIIGGKSVGSLHGFMHKYLKNTIIYVFGIEILKNMIFV-IAATTR- 120
           E +LFEC YP +   ++G  S+  + G +HK + +  +      I+K+ + + I    R 
Sbjct: 96  EGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRF 155

Query: 121 -LKKWASHNVVELKDEIANMIFDLTAKRLISYDPEKSSENLRENFVAFIEGLISFPLHIP 180
            L  W+S   V L +E   + F+LT K+L+S+DP + SE+LR+ ++  IEG  S PL + 
Sbjct: 156 NLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLF 215

Query: 181 GTSFHKCLQ 187
            T++ K +Q
Sbjct: 216 STTYRKAIQ 222

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004141154.18.3e-8382.01cytochrome P450 87A3 isoform X1 [Cucumis sativus][more]
XP_031737523.18.3e-8382.01cytochrome P450 87A3 isoform X2 [Cucumis sativus][more]
XP_022935245.11.2e-7673.94cytochrome P450 87A3-like isoform X2 [Cucurbita moschata][more]
XP_022935244.11.2e-7673.94cytochrome P450 87A3-like isoform X1 [Cucurbita moschata][more]
XP_022983296.14.4e-7673.40cytochrome P450 87A3-like isoform X3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q7XU389.3e-6157.37Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV... [more]
K7NBR26.9e-3236.32Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=... [more]
O649891.8e-2733.33Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2[more]
Q425692.3e-2735.45Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
Q6F4F55.7e-2634.85Cytochrome P450 724B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP724B1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LCN34.0e-8382.01Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G845470 PE=3 SV=1[more]
A0A6J1FA365.6e-7773.94cytochrome P450 87A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114421... [more]
A0A6J1F4V65.6e-7773.94cytochrome P450 87A3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114421... [more]
A0A6J1J5G82.1e-7673.40cytochrome P450 87A3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481917... [more]
A0A6J1J1Q72.1e-7673.40cytochrome P450 87A3-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481917... [more]
Match NameE-valueIdentityDescription
AT1G12740.11.3e-6560.64cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
AT1G12740.22.1e-6358.25cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
AT3G50660.11.3e-2833.33Cytochrome P450 superfamily protein [more]
AT5G05690.11.6e-2835.45Cytochrome P450 superfamily protein [more]
AT5G05690.21.6e-2835.45Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..187
e-value: 5.6E-28
score: 99.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 2..176
NoneNo IPR availablePANTHERPTHR24286:SF11CYTOCHROME P450, FAMILY 87, SUBFAMILY A, POLYPEPTIDE 2coord: 1..186
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 1..186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC10G191590.1CaUC10G191590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen