CaUC09G170680 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC09G170680
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	Serine proteinase inhibitor
Location	Ciama_Chr09: 17558987 .. 17559315 (-)
RNA-Seq Expression	CaUC09G170680
Synteny	CaUC09G170680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTACCATCGTAAGTTTATCTTCTTGTAATATTTTGGTTGAGTTCATCTTCCTTTTTAAATATACTTTTAATCTTAACTACTTAACAAATTCTTTTTTTTTCTTTCTTCTTTTTCTGCATATGTTGGAGAAAAGTGAAATCGTGGCCTGCACTGATCTTCATGAATGCAGACACGGTTGCAAATATCATCAAGAAAGAACATCCTGACTTTCAGATGATCAAAGTGCTGGCAGGAACTCCAGTAACAAAGGATCTTAAACATGGTCGAGTTCGATTATTTATAAATGTAAAGGAAGATGTGGTTGAAATTCCTCAAGAGGGCTAA

mRNA sequence

ATGTCTTACCATCTGAAATCGTGGCCTGCACTGATCTTCATGAATGCAGACACGGTTGCAAATATCATCAAGAAAGAACATCCTGACTTTCAGATGATCAAAGTGCTGGCAGGAACTCCAGTAACAAAGGATCTTAAACATGGTCGAGTTCGATTATTTATAAATGTAAAGGAAGATGTGGTTGAAATTCCTCAAGAGGGCTAA

Coding sequence (CDS)

ATGTCTTACCATCTGAAATCGTGGCCTGCACTGATCTTCATGAATGCAGACACGGTTGCAAATATCATCAAGAAAGAACATCCTGACTTTCAGATGATCAAAGTGCTGGCAGGAACTCCAGTAACAAAGGATCTTAAACATGGTCGAGTTCGATTATTTATAAATGTAAAGGAAGATGTGGTTGAAATTCCTCAAGAGGGCTAA

Protein sequence

MSYHLKSWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVEIPQEG

Homology

[Close]

BLAST of CaUC09G170680 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 101.7 bits (252), Expect = 2.6e-18
Identity = 48/65 (73.85%), Postives = 57/65 (87.69%), Query Frame = 0


Query: 3  YHLKSWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVE 62
          + LKSWPAL FM+A TVANIIKKE+PDFQ+  +LAG+PVTKDLKHGRVRLF N++E VV 
Sbjct: 7  HQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVI 66

Query: 63 IPQEG 68
          +PQEG
Sbjct: 67 VPQEG 71

[Close]

BLAST of CaUC09G170680 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 99.4 bits (246), Expect = 1.3e-17
Identity = 45/67 (67.16%), Postives = 56/67 (83.58%), Query Frame = 0


Query: 1  MSYHLKSWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDV 60
          M+Y LKSWP L  M A TV NIIK++HP+FQ++ +LAG+PVTKDLKHGRVRLF+N+   V
Sbjct: 1  MAYVLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIV 60

Query: 61 VEIPQEG 68
          VEIP+EG
Sbjct: 61 VEIPREG 67

[Close]

BLAST of CaUC09G170680 vs. NCBI nr
Match: KGN60478.1 (hypothetical protein Csa_001396 [Cucumis sativus])

HSP 1 Score: 82.4 bits (202), Expect = 1.6e-12
Identity = 37/65 (56.92%), Postives = 50/65 (76.92%), Query Frame = 0


Query: 3  YHLKSWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVE 62
          ++LKSWPAL F++A TVAN IK E+P+F+++ V  GTPVTKD+  GRVRL ++    VV 
Sbjct: 5  FNLKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVN 64

Query: 63 IPQEG 68
          +PQEG
Sbjct: 65 VPQEG 69

[Close]

BLAST of CaUC09G170680 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 67.8 bits (164), Expect = 4.1e-08
Identity = 26/61 (42.62%), Postives = 45/61 (73.77%), Query Frame = 0


Query: 7  SWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVEIPQE 66
          SWP L+F+ +    + I+++ PD + + +LAG+PVT+DL+  RVR+F+N+ + VVE+P+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 67 G 68
          G
Sbjct: 75 G 75

[Close]

BLAST of CaUC09G170680 vs. NCBI nr
Match: XP_006490176.1 (inhibitor of trypsin and hageman factor-like [Citrus sinensis])

HSP 1 Score: 61.6 bits (148), Expect = 2.9e-06
Identity = 28/61 (45.90%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 7   SWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVEIPQE 66
           SWP L+  N +  A II+KE+P    I +L GTPVTKD +  RVR+++N K  V+ +P+ 
Sbjct: 68  SWPELVGKNGEVAAAIIEKENPCVHAIVLLEGTPVTKDYRIDRVRVWVNKKGKVIRVPRI 127

Query: 67  G 68
           G
Sbjct: 128 G 128

[Close]

BLAST of CaUC09G170680 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.3e-08
Identity = 30/61 (49.18%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 8   WPALIFMNADTVANIIKKEHPDFQMIK-VLAGTPVTKDLKHGRVRLFINVKEDVVEIPQE 67
           WP L+   A     II+KE+P    ++ VL GTPVT+DL+  RVRLF+NV + VV+ PQ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQTPQV 106

[Close]

BLAST of CaUC09G170680 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.2e-07
Identity = 29/59 (49.15%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 8   WPALIFMNADTVANIIKKEHPDFQMIKVLA-GTPVTKDLKHGRVRLFINVKEDVVEIPQ 66
           WP LI + A     II+KE+P    I +L  G+PVTKD +  RVRLF+N+  DVV+IP+
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIPR 109

[Close]

BLAST of CaUC09G170680 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.6e-07
Identity = 29/63 (46.03%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 6  KSWPALIFMNADTVANIIKKEHPDFQMI-KVLAGTPVTKDLKHGRVRLFINVKEDVVEIP 65
          ++WP LI + A     II+KE+     +  VL G+PVTKD +  RVRLF+NV + VV+IP
Sbjct: 32 ETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQIP 91

Query: 66 QEG 68
          + G
Sbjct: 92 RVG 94

[Close]

BLAST of CaUC09G170680 vs. ExPASy Swiss-Prot
Match: Q03198 (Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 6.2e-07
Identity = 27/61 (44.26%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 8   WPALIFMNADTVANIIKKEHPDFQMIK-VLAGTPVTKDLKHGRVRLFINVKEDVVEIPQE 67
           WP L+   A     II+KE+P    ++ +L G PVT+DL+  RVRLF+NV + +V+ PQ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTILNGGPVTEDLRCNRVRLFVNVLDFIVQTPQI 106

[Close]

BLAST of CaUC09G170680 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 8.0e-07
Identity = 24/61 (39.34%), Postives = 36/61 (59.02%), Query Frame = 0


Query: 7  SWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVEIPQE 66
          SWP L+  N D  A II+ E+ + + I V  G P+T+DL   RVR+F++    V ++P  
Sbjct: 10 SWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENRVVTQVPAI 69

Query: 67 G 68
          G
Sbjct: 70 G 70

[Close]

BLAST of CaUC09G170680 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-18
Identity = 48/65 (73.85%), Postives = 57/65 (87.69%), Query Frame = 0


Query: 3  YHLKSWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVE 62
          + LKSWPAL FM+A TVANIIKKE+PDFQ+  +LAG+PVTKDLKHGRVRLF N++E VV 
Sbjct: 7  HQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVI 66

Query: 63 IPQEG 68
          +PQEG
Sbjct: 67 VPQEG 71

[Close]

BLAST of CaUC09G170680 vs. ExPASy TrEMBL
Match: A0A0A0LFD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 7.8e-13
Identity = 37/65 (56.92%), Postives = 50/65 (76.92%), Query Frame = 0


Query: 3  YHLKSWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVE 62
          ++LKSWPAL F++A TVAN IK E+P+F+++ V  GTPVTKD+  GRVRL ++    VV 
Sbjct: 5  FNLKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVN 64

Query: 63 IPQEG 68
          +PQEG
Sbjct: 65 VPQEG 69

[Close]

BLAST of CaUC09G170680 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.0e-08
Identity = 26/61 (42.62%), Postives = 45/61 (73.77%), Query Frame = 0


Query: 7  SWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVEIPQE 66
          SWP L+F+ +    + I+++ PD + + +LAG+PVT+DL+  RVR+F+N+ + VVE+P+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 67 G 68
          G
Sbjct: 75 G 75

[Close]

BLAST of CaUC09G170680 vs. ExPASy TrEMBL
Match: Q8GT64 (Type I proteinase inhibitor-like protein OS=Citrus paradisi OX=37656 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-06
Identity = 28/61 (45.90%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 7   SWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVEIPQE 66
           SWP L+  N +  A II+KE+P    I +L GTPVTKD +  RVR+++N K  V+ +P+ 
Sbjct: 68  SWPELVGKNGEVAAAIIEKENPCVHAIVLLEGTPVTKDYRIDRVRVWVNKKGKVIRVPRI 127

Query: 67  G 68
           G
Sbjct: 128 G 128

[Close]

BLAST of CaUC09G170680 vs. ExPASy TrEMBL
Match: A0A067E312 (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033055mg PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 3.2e-06
Identity = 27/61 (44.26%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 7   SWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVEIPQE 66
           SWP L+  N +  A II+KE+P    I +L GTPVT+D +  RVR+++N K  V+ +P+ 
Sbjct: 68  SWPELVGKNGEVAAAIIEKENPCVHAIVLLEGTPVTRDYRIDRVRVWVNKKGKVIRVPRI 127

Query: 67  G 68
           G
Sbjct: 128 G 128

[Close]

BLAST of CaUC09G170680 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.8 bits (125), Expect = 1.3e-07
Identity = 24/61 (39.34%), Postives = 36/61 (59.02%), Query Frame = 0


Query: 7  SWPALIFMNADTVANIIKKEHPDFQMIKVLAGTPVTKDLKHGRVRLFINVKEDVVEIPQE 66
          SWP L   N D  A +I++E+P      +L G+PVT D +  RVR+F++    VV+ P+ 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKS 69

Query: 67 G 68
          G
Sbjct: 70 G 70

The following BLAST results are available for this feature:

BLAST of CaUC09G170680 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN60477.1	2.6e-18	73.85	hypothetical protein Csa_001998 [Cucumis sativus]	[more]
KAG6571807.1	1.3e-17	67.16	hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN60478.1	1.6e-12	56.92	hypothetical protein Csa_001396 [Cucumis sativus]	[more]
XP_011654473.1	4.1e-08	42.62	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
XP_006490176.1	2.9e-06	45.90	inhibitor of trypsin and hageman factor-like [Citrus sinensis]	[more]

BLAST of CaUC09G170680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q03199	4.3e-08	49.18	Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1	[more]
P16231	1.2e-07	49.15	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
Q02214	3.6e-07	46.03	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
Q03198	6.2e-07	44.26	Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1	[more]
Q6XNP7	8.0e-07	39.34	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]

BLAST of CaUC09G170680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIF6	1.2e-18	73.85	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1	[more]
A0A0A0LFD4	7.8e-13	56.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1	[more]
A0A0A0KPI0	2.0e-08	42.62	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
Q8GT64	1.4e-06	45.90	Type I proteinase inhibitor-like protein OS=Citrus paradisi OX=37656 PE=2 SV=1	[more]
A0A067E312	3.2e-06	44.26	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g033055mg PE=3 SV=1	[more]

BLAST of CaUC09G170680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	1.3e-07	39.34	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 7..65 e-value: 4.0E-13 score: 49.8
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 6..67
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 6..67 e-value: 2.9E-14 score: 55.3
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 6..67
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 5..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC09G170680.1	CaUC09G170680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity