CaUC09G169850 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC09G169850
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	beta-amyrin 11-oxidase-like
Location	Ciama_Chr09: 14078273 .. 14081560 (-)
RNA-Seq Expression	CaUC09G169850
Synteny	CaUC09G169850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAAGAAAAGAGATAGGAAATATCTTAGAAAGTGTGATAGAGGAGAAAAGAAAAGCACTAGAAAAGAAAGAAACAACAGAAGTGAAATGCCAAATGGAGAAATTGATTGTGGCCACTGATGAAAATGGGACAAAATTATATGATAATAATGCAATTATAGACTTGCTTCTTGGATTATTACATGCTGGCCATCATACTCCAGCTTATGCTGCTATGTGGGCTCTTGTACACATCTCAGAAAATTCTCATGTCTTCCAAAAAGCCAAGGTGTGATTCAAAAAGCACTTTTGATTTTCTCCATTTTTCTTTCCCACTTCTTCTTTTGTGATATTTTTCAATTTTTGGTCATTCACAAGTAATTGTGGTTTTTTTTGATATTTGAGCATTTTACAGGAAGAGCAAGAGTTTATTATAAAACAAAGGCCATCTACTCAAAAGGGATTGACTTTTAAGGAAATCAAACAAATGAAATATCTTATGAAGGTGTTGTGTGCTTTTCTAGTTAATTATATCTCTTTTTTATGTTTTTTTTTTTTTTTTTAAAGTATAATAGGGGTGGGAGATTTAAACTATGAACCTGTTCGTAATTGCTAATACATGCTAGTTGAGCTACAATTGCTTTGACTATAACTCCTCCTAATATAAAACTAAAAGAGAAAATAAAAAAAAAATGTTGAAAATAGAAAATAGAAAGTTAGGTCTTGGTTAGTAACAATTTAGACCTCATTTGGTAACCATTTTATTTTTGAATTTTTGAAAATTAGATCTATTTCCTCATATTTTCTTACCATAGTTTGCATCTTTCTTAAATACAATAGTTGAATTCTTAACCAATTTCAAAAAACAAAAACAAGTTTTTAAAATTTATTTTTTTTGGTTTACAAAATTTGGCTTGTTTTCTACAACATGGGTAAAAAGTAGATTTTAGTTTGGTTGTTAAGATCACTTTAACAAGAATATAAAACTAAATGAGGGGTGTTTGTAGGCTTAATTTTCAAAAACTAAAAACCAAACATCAAATGAACGGGGCTTTAGATTTTAGTTTTTGTTTTTGAATTTATGCTTGGTTTCTTGTAAATTTCATAGTATGGTTCTTACGTTTCTTGACAAAACACTTGAATTTTTAGTCAAATTATGAACACAAAAACAAAGTTTTTAAAAACCACCTTTTTAAGCTTTTCAAGTTTTGCTTGGTTTTTTAAAAACATTAGTGAAATGTAGATAATTGAACATGAAAGCTTATAAGTGAAAATAGTGTTTATAAATTTGATTTTTGAAAACTAAACACTAATAGTCAAATGGTTACTTAATGAGGTCTAAATTATTCGGTTAAAAAAAAAAAAAAAAAAATGTGAAACCGATTAAAATTGAAGTAAGAGAAATAAATAACATAGTTGGACTTCTAAAATATGAAAGGCCATATATTAACACTTGTGAAAAGTAAATTATAAGATACTTCTTTCCTTCCGAGAGCTAAATGCCAATTATTCTAGCAATATTGACTAAACAATGAAGTGAAAGTTTACCAAATAGGGAAACATATGACATAAGAAAACTTTATAATAATTATTATTATTTCTTTTACCTGATTGACTCGAAAACAGGAAGTAATTAACTTGGATTGCAAATTATTCCAACCTAAATATGTAGAAAATTAACCATCATAAAATACACATAAATATGTAGCAAGAATCTAGCCTCATAAAATAATAATAAGATTTGAAAGTGTGTGGTTCATTTTTTAGATTACATAGACATATTAGGTGACACCCGAGCAAAGAAGAATTACACTAATTGATTGTGACCACATCCTAAAATAAGATAGATCTTGAAGATATATAAGTTTGAAAAAATTGATAGATATTTTTTATACACCTTATTTTTCACTGACTCAATCAATAAAAATGTCAAAATTAGCCTAACGCATCTTAGAACAATTCTATATTGCGAGATCTCTTGAAAATTATCTTAGAAAGAGAATAAGAGACAATTTACCACATCATATGAACTATTCAAATAACTAATTAAAATATAGTCTTCTAAACTTACATGTTAAGATCACTTTAGCAAGAATATAAAGTCAACCATCTAATTCAATCTATATTTGAAATAACATTGAGAAAATAAATATAAAAATGGTTTGATTTATTTGTTATTTCAGTTCATTAATGAGCTATTGAGAAGACATACCATTGCTGTCGCAAGTTTTCGTGAGGCAAAAACAGATGTATACATCAATGGTTAGTTATTCTATATATATTATTATAATTATTACATTATACATGATATTGATTATTTCTTTGTTGATAATAATTTATTTTGTTCTCCAACACTATTATTTTTATTTTTATTTTTCTTCCTTGTTTGTTTTAGACTATACTATACCCAAAGGATGGACAGTACCAATTTGGACTAGAGCTATTCATATGGACCCTCAAATATATTCAAATCCACAAGAATTTGATCCTTCAAGATGGGATGTATGTTTTATGATTATTCATTCAATTCATTAAAAAAAATCCACACATGAACTTAGAAAAAATAAAGATATATACTTATATACAATTAATTTTCATGTTGAAATATATATACACACACATATACATATTGTGGAATCAAAGATATCAATATACTAGGTTTTTGAAAAGTAAATATCAGAAAATATTTGAAAGGAGGTTTGTTGATAAATAAGAAGGTTTTTTTAAAAGGAAGAATCAAAGAGCACCTAGCAAATATAGCAATAGATTCAAAGTATTAGAAAATATAGCACAATTAAAAAGAATTTGTAAATATAACATAATTTAGATTCAACTCCCAAAATCTATCAATGATAGATGAGATATCGCTAAGAGAAGTATACTAGTGATAGATCATATTGCTAATAGGAATCTAGTAGCATCAACGATAGAGTCTATTATCAATAGATTTTACTATATTTGCAATTTTTATATATACTTAATTACTAACTCTAAAATGTTATTGCAATTATTGGACAGAATTATACACCAAAACCAGGAGAATTCATCCCTTTTGGAATTGGAAGTAGGTTTTGTCCTGGAAGTGAACTTACCAAGCTTGAGATTACAATTTTACTTCATCATTTTATTCTCAATTACAAGTAAGTATTCTTTTTTCGGTTAAATTGAAAATAAATAAATTTGTAATGGAATTGTTTTGTTTATTTATTGTTGTAGAATGGAACGAGTCAATCCAAGATGCGACATCACTCATTTACCTGCCCCCAAGCTAGTAGACAATTGCCTTTGCAGGATTATCAAGCTCCCATAG

mRNA sequence

GCAAGAAAAGAGATAGGAAATATCTTAGAAAGTGTGATAGAGGAGAAAAGAAAAGCACTAGAAAAGAAAGAAACAACAGAAGTGAAATGCCAAATGGAGAAATTGATTGTGGCCACTGATGAAAATGGGACAAAATTATATGATAATAATGCAATTATAGACTTGCTTCTTGGATTATTACATGCTGGCCATCATACTCCAGCTTATGCTGCTATGTGGGCTCTTGTACACATCTCAGAAAATTCTCATGTCTTCCAAAAAGCCAAGTTCATTAATGAGCTATTGAGAAGACATACCATTGCTGTCGCAAGTTTTCGTGAGGCAAAAACAGATGTATACATCAATGACTATACTATACCCAAAGGATGGACAGTACCAATTTGGACTAGAGCTATTCATATGGACCCTCAAATATATTCAAATCCACAAGAATTTGATCCTTCAAGATGGGATAATTATACACCAAAACCAGGAGAATTCATCCCTTTTGGAATTGGAAGTAGGTTTTGTCCTGGAAGTGAACTTACCAAGCTTGAGATTACAATTTTACTTCATCATTTTATTCTCAATTACAAAATGGAACGAGTCAATCCAAGATGCGACATCACTCATTTACCTGCCCCCAAGCTAGTAGACAATTGCCTTTGCAGGATTATCAAGCTCCCATAG

Coding sequence (CDS)

GCAAGAAAAGAGATAGGAAATATCTTAGAAAGTGTGATAGAGGAGAAAAGAAAAGCACTAGAAAAGAAAGAAACAACAGAAGTGAAATGCCAAATGGAGAAATTGATTGTGGCCACTGATGAAAATGGGACAAAATTATATGATAATAATGCAATTATAGACTTGCTTCTTGGATTATTACATGCTGGCCATCATACTCCAGCTTATGCTGCTATGTGGGCTCTTGTACACATCTCAGAAAATTCTCATGTCTTCCAAAAAGCCAAGTTCATTAATGAGCTATTGAGAAGACATACCATTGCTGTCGCAAGTTTTCGTGAGGCAAAAACAGATGTATACATCAATGACTATACTATACCCAAAGGATGGACAGTACCAATTTGGACTAGAGCTATTCATATGGACCCTCAAATATATTCAAATCCACAAGAATTTGATCCTTCAAGATGGGATAATTATACACCAAAACCAGGAGAATTCATCCCTTTTGGAATTGGAAGTAGGTTTTGTCCTGGAAGTGAACTTACCAAGCTTGAGATTACAATTTTACTTCATCATTTTATTCTCAATTACAAAATGGAACGAGTCAATCCAAGATGCGACATCACTCATTTACCTGCCCCCAAGCTAGTAGACAATTGCCTTTGCAGGATTATCAAGCTCCCATAG

Protein sequence

ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLLHAGHHTPAYAAMWALVHISENSHVFQKAKFINELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKLVDNCLCRIIKLP

Homology

[Close]

BLAST of CaUC09G169850 vs. NCBI nr
Match: XP_038877829.1 (beta-amyrin 11-oxidase-like [Benincasa hispida])

HSP 1 Score: 412.5 bits (1059), Expect = 2.3e-111
Identity = 206/250 (82.40%), Postives = 210/250 (84.00%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARKEIGNIL+ VIEEKR+ LE KET EVK QM+KLIVATDENG KLYDNN IIDLLLGLL
Sbjct: 236 ARKEIGNILDCVIEEKRRVLENKETREVKSQMDKLIVATDENGKKLYDNNDIIDLLLGLL 295

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQK----------------------------AKFIN 120
           HAGHHTPAYAAMWALV ISEN+HVFQK                             KFIN
Sbjct: 296 HAGHHTPAYAAMWALVQISENAHVFQKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFIN 355

Query: 121 ELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDN 180
           ELLRRHTIAVASFREAKTDVYINDY IPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDN
Sbjct: 356 ELLRRHTIAVASFREAKTDVYINDYIIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDN 415

Query: 181 YTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKLVD 223
           YTPKPG FIPFGIGSRFCPGSELTKL ITILLHHFILNYKMERVNPRCDITHLPAPKLVD
Sbjct: 416 YTPKPGAFIPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDITHLPAPKLVD 475

[Close]

BLAST of CaUC09G169850 vs. NCBI nr
Match: XP_011652661.1 (beta-amyrin 11-oxidase [Cucumis sativus])

HSP 1 Score: 385.2 bits (988), Expect = 3.9e-103
Identity = 193/253 (76.28%), Postives = 204/253 (80.63%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTE--VKCQMEKLIVATDENGTKLYDNNAIIDLLLG 60
           ARKEI NILE VI EKR+ LE+KET +  VKCQM+KLIVATDENG KLYDN AIIDLLLG
Sbjct: 237 ARKEIENILEGVIMEKRRVLERKETNDELVKCQMDKLIVATDENGKKLYDNKAIIDLLLG 296

Query: 61  LLHAGHHTPAYAAMWALVHISENSHVFQKA------------------------------ 120
           LLHAGHHTPAY AMWA+V IS+NSH+FQKA                              
Sbjct: 297 LLHAGHHTPAYTAMWAIVQISQNSHIFQKAKEEQELIMKERPSTQKGLSFTEIKQMKYLM 356

Query: 121 KFINELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPS 180
           KF NELLRRHT+AVASFREA  +V+INDYTIPKGWTV IWTRAIHMDPQIYSNPQEFDPS
Sbjct: 357 KFTNELLRRHTVAVASFREATANVHINDYTIPKGWTVTIWTRAIHMDPQIYSNPQEFDPS 416

Query: 181 RWDNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAP 222
           RWDNYTPKPGEF+PFGIGSRFCPGSELTKL ITILLH FILNYKMERVN RCD THLPAP
Sbjct: 417 RWDNYTPKPGEFLPFGIGSRFCPGSELTKLYITILLHQFILNYKMERVNQRCDTTHLPAP 476

[Close]

BLAST of CaUC09G169850 vs. NCBI nr
Match: XP_023511598.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 378.3 bits (970), Expect = 4.7e-101
Identity = 187/252 (74.21%), Postives = 204/252 (80.95%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK+I N+LE VIEEKR+ALEKKET EV  QM+KLI+A DENG KLYDNNAIIDLLLGLL
Sbjct: 84  ARKKIENVLECVIEEKRRALEKKETREVHSQMDKLIMARDENGMKLYDNNAIIDLLLGLL 143

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKA------------------------------KF 120
           H GHHTPAYAAMWALVHISEN H+F +A                              KF
Sbjct: 144 HGGHHTPAYAAMWALVHISENPHIFYEAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKF 203

Query: 121 INELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRW 180
           INELLRR+TIAVASFR+AKTD+ INDYTIPKGW V IWTRAIHMDP +YSNPQEFDPSRW
Sbjct: 204 INELLRRNTIAVASFRKAKTDININDYTIPKGWNVLIWTRAIHMDPHLYSNPQEFDPSRW 263

Query: 181 DNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKL 223
           +N++PKPG FIPFGIG+RFCPGSELTKLEITILLHHFILNYKMERVNP+C I+HLPAPKL
Sbjct: 264 ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYKMERVNPKCYISHLPAPKL 323

[Close]

BLAST of CaUC09G169850 vs. NCBI nr
Match: XP_023511599.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375.2 bits (962), Expect = 4.0e-100
Identity = 187/252 (74.21%), Postives = 206/252 (81.75%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK+I NIL+ VIEEKR+ALEK+E  EV+ QM+KLI+A DENG KLYDNNAIIDLLLGLL
Sbjct: 236 ARKKIENILQCVIEEKRRALEKQE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLL 295

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKA------------------------------KF 120
           H GHHTPAYAAMWALVHISEN H+F +A                              KF
Sbjct: 296 HGGHHTPAYAAMWALVHISENPHIFYQAKEEQESILKQRPSTQKGLTFQKIKQMKYLMKF 355

Query: 121 INELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRW 180
           INELLRR+TIAVASFREAKTD+ INDYTIPKGWTV IWTRAIHMDPQ+YSNP+EFDPSRW
Sbjct: 356 INELLRRNTIAVASFREAKTDININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDPSRW 415

Query: 181 DNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKL 223
           +N++PKPG FIPFGIG+RFCPGSELTKLE+TILLHHFILNYKMERVNP+C ITHLPAPKL
Sbjct: 416 ENHSPKPGTFIPFGIGTRFCPGSELTKLELTILLHHFILNYKMERVNPKCCITHLPAPKL 475

[Close]

BLAST of CaUC09G169850 vs. NCBI nr
Match: XP_022952148.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 373.6 bits (958), Expect = 1.2e-99
Identity = 187/252 (74.21%), Postives = 205/252 (81.35%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK+I NIL+ VIEEKR+ALEK+E  EV+ QM+KLI+A DENG KLYDNNAIIDLLLGLL
Sbjct: 236 ARKKIENILQCVIEEKRRALEKQE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLL 295

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKA------------------------------KF 120
           H GHHTPAYAAMWALVHISE  HVF KA                              KF
Sbjct: 296 HGGHHTPAYAAMWALVHISETPHVFHKAKEEQESILKQRSSTQKGLTFQEIKQMKYLMKF 355

Query: 121 INELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRW 180
           INELLRR+TIAVASFREAKT++ INDYTIPKGWTV IWTRAIHMDPQ+YSNP+EFDPSRW
Sbjct: 356 INELLRRNTIAVASFREAKTEININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDPSRW 415

Query: 181 DNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKL 223
           +N++PKPG FIPFGIG+RFCPGSELTKLE+TILLHHFILNYKMERVNP+C ITHLPAPKL
Sbjct: 416 ENHSPKPGTFIPFGIGTRFCPGSELTKLELTILLHHFILNYKMERVNPKCCITHLPAPKL 475

[Close]

BLAST of CaUC09G169850 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 5.1e-58
Identity = 114/253 (45.06%), Postives = 160/253 (63.24%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEK--KETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLG 60
           ARK++  I++ V++E+R  +E   +E ++ K  ++ L+   DENG KL D + I DLL+G
Sbjct: 239 ARKKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDED-ISDLLIG 298

Query: 61  LLHAGHHTPAYAAMWALVHISENSHVFQKAK----------------------------- 120
           LL AGH + A + MW++ +++++ H+ +KAK                             
Sbjct: 299 LLFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLS 358

Query: 121 -FINELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPS 180
             I+E LR   IA A+FREA  DV IN Y IPKGW V IW RAIHMD + Y NP+EF+PS
Sbjct: 359 QVIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPS 418

Query: 181 RWDNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAP 222
           RWD+Y  K G F+PFG GSR CPG++L KLEI+I LH+F+ NY++ER+NP C +T LP  
Sbjct: 419 RWDDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVTSLPVS 478

[Close]

BLAST of CaUC09G169850 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 4.5e-54
Identity = 104/251 (41.43%), Postives = 154/251 (61.35%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           AR+ +  +L+ V++E+R A  K  +      M++LI A DE G  L D++ IID+L+  L
Sbjct: 268 ARRRLVAVLQGVLDERRAARAKGVSGGGVDMMDRLIEAQDERGRHL-DDDEIIDVLVMYL 327

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKAK------------------------------F 120
           +AGH +  +  MWA V + EN  +F +AK                               
Sbjct: 328 NAGHESSGHITMWATVFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQV 387

Query: 121 INELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRW 180
           I+E LR   I+  SFR+A  DV++N Y IPKGW V +W R++HMDPQ+Y +P +FDPSRW
Sbjct: 388 IDETLRLVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRW 447

Query: 181 DNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKL 222
           + ++P+ G F+ FG+G+R CPG++L KLEI++ LHHF+L YK+ R NPRC + +LP P+ 
Sbjct: 448 EGHSPRAGTFLAFGLGARLCPGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRP 507

[Close]

BLAST of CaUC09G169850 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 4.2e-52
Identity = 100/252 (39.68%), Postives = 154/252 (61.11%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKK-ETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGL 60
           AR+++ ++L+ V++ +R A  K  + +     M++LI A DE G +L D+  I+D+L+  
Sbjct: 252 ARRKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDE-IVDVLIMY 311

Query: 61  LHAGHHTPAYAAMWALVHISENSHVFQKAK------------------------------ 120
           L+AGH +  +  MWA V + EN  +F +AK                              
Sbjct: 312 LNAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQ 371

Query: 121 FINELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSR 180
            ++E LR   I+  SFR+A  D+++N Y IPKGW V +W R++HMD Q+Y +P+ F+PSR
Sbjct: 372 VVDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSR 431

Query: 181 WDNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPK 222
           W+   PK G F+PFG+G+R CPG++L KLEI++ LHHF+L YK++R NP+C + +LP P+
Sbjct: 432 WEGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPR 491

[Close]

BLAST of CaUC09G169850 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 7.2e-52
Identity = 103/249 (41.37%), Postives = 150/249 (60.24%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK++    +S++  +R   ++  ++  K  ++ LI   DENG ++ D+  IIDLLL  L
Sbjct: 239 ARKKLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYL 298

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKAK-----------------------------FI 120
           +AGH +  +  MWA + + E+  + QKAK                              I
Sbjct: 299 NAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQKLTLKETREMVYLSQVI 358

Query: 121 NELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWD 180
           +E LR  T ++ +FREAK+DV ++ Y IPKGW V  W R +H+DP+IY +P++FDPSRW+
Sbjct: 359 DETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 418

Query: 181 NYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKLV 221
            YTPK G F+PFG+GS  CPG++L KLEI+I LHHF+L Y++ER NP C +  LP  +  
Sbjct: 419 GYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPK 478

[Close]

BLAST of CaUC09G169850 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.1e-51
Identity = 102/253 (40.32%), Postives = 148/253 (58.50%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVK--CQMEKLIVATDENGTKLYDNNAIIDLLLG 60
           AR+ +   L+ V++E+R    K           M++LI   DE G +L D+  IID+L+ 
Sbjct: 243 ARRRLVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDE-IIDVLVM 302

Query: 61  LLHAGHHTPAYAAMWALVHISENSHVFQKAK----------------------------- 120
            L+AGH +  +  MWA V + EN  +  KAK                             
Sbjct: 303 YLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLS 362

Query: 121 -FINELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPS 180
             ++E LR   I+  SFR+A  DV++N Y IPKGW V +W R++HMDPQ+Y +P++FDPS
Sbjct: 363 QVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPS 422

Query: 181 RWDNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAP 222
           RW+   P+ G F+PFG+G+R CPG++L KLEI++ LHHF+L YK+ R NP C + +LP P
Sbjct: 423 RWEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPHP 482

[Close]

BLAST of CaUC09G169850 vs. ExPASy TrEMBL
Match: A0A6J1GJP8 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.6e-100
Identity = 187/252 (74.21%), Postives = 205/252 (81.35%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK+I NIL+ VIEEKR+ALEK+E  EV+ QM+KLI+A DENG KLYDNNAIIDLLLGLL
Sbjct: 236 ARKKIENILQCVIEEKRRALEKQE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLL 295

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKA------------------------------KF 120
           H GHHTPAYAAMWALVHISE  HVF KA                              KF
Sbjct: 296 HGGHHTPAYAAMWALVHISETPHVFHKAKEEQESILKQRSSTQKGLTFQEIKQMKYLMKF 355

Query: 121 INELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRW 180
           INELLRR+TIAVASFREAKT++ INDYTIPKGWTV IWTRAIHMDPQ+YSNP+EFDPSRW
Sbjct: 356 INELLRRNTIAVASFREAKTEININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDPSRW 415

Query: 181 DNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKL 223
           +N++PKPG FIPFGIG+RFCPGSELTKLE+TILLHHFILNYKMERVNP+C ITHLPAPKL
Sbjct: 416 ENHSPKPGTFIPFGIGTRFCPGSELTKLELTILLHHFILNYKMERVNPKCCITHLPAPKL 475

[Close]

BLAST of CaUC09G169850 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.6e-99
Identity = 188/252 (74.60%), Postives = 203/252 (80.56%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK+I NIL+ VIEEKR+ALEK+E  EV+ QM+KLI+A DENG KLYDNNAIIDLLLGLL
Sbjct: 236 ARKKIENILQCVIEEKRRALEKEE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLL 295

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKA------------------------------KF 120
           HAGHHTPAYAAMWALVHISE  HVF KA                              KF
Sbjct: 296 HAGHHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKF 355

Query: 121 INELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRW 180
           INELLRR+TIAVASFR+AK DV INDY IPKGW V IWTRAIHMDPQ+YSNPQEFDPSRW
Sbjct: 356 INELLRRNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRW 415

Query: 181 DNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKL 223
           +NY+PKPG F+PFGIG+RFCPGSELTKLEITILLHHFILNYKMERVNP+C ITHLPAPKL
Sbjct: 416 ENYSPKPGTFLPFGIGTRFCPGSELTKLEITILLHHFILNYKMERVNPKCCITHLPAPKL 475

[Close]

BLAST of CaUC09G169850 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.1e-99
Identity = 182/252 (72.22%), Postives = 202/252 (80.16%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK+I N+LE VIEEKR+ALE+KET EV  QM+KLI+A DENG KLYDNN IIDLLLGLL
Sbjct: 236 ARKKIENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLL 295

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKA------------------------------KF 120
           HAGHHTPAYAAMWALVHISE  H+F KA                              KF
Sbjct: 296 HAGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKF 355

Query: 121 INELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRW 180
           INELLRR+TI VASFR+AKTD+ INDY IPKGW+V IWTRA+HMDPQ+YSNP+EFDPSRW
Sbjct: 356 INELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRW 415

Query: 181 DNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKL 223
           +N++PKPG FIPFGIG+RFCPGSELTKLEITILLHHFILNYKMER NP+C I+HLPAPKL
Sbjct: 416 ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYKMERTNPKCCISHLPAPKL 475

[Close]

BLAST of CaUC09G169850 vs. ExPASy TrEMBL
Match: A0A6J1I8I5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.9e-96
Identity = 185/261 (70.88%), Postives = 203/261 (77.78%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK+I NIL+ VIEEKR+ALEK+E  EV+ QM+KLI+A DENG KLYDNNAIIDLLLGLL
Sbjct: 236 ARKKIENILQCVIEEKRRALEKQE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLL 295

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKA------------------------------KF 120
           H GHHTPAYAAMWALV I E  HVF KA                              KF
Sbjct: 296 HGGHHTPAYAAMWALVQIFETPHVFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKF 355

Query: 121 INELLRRHTIAVASFREAKTDVYIN---------DYTIPKGWTVPIWTRAIHMDPQIYSN 180
           INELLRR+TIAVASFREAKTD+ IN         DYTIPKGWTV IWTRAIHMDPQ+YSN
Sbjct: 356 INELLRRNTIAVASFREAKTDININGELFYYIVLDYTIPKGWTVLIWTRAIHMDPQLYSN 415

Query: 181 PQEFDPSRWDNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCD 223
           P+EFDPSRW+N++PKPG FIPFG+G+RFCPGSELTKLE+TILLHHFILNYKMERVNP+C 
Sbjct: 416 PKEFDPSRWENHSPKPGTFIPFGMGTRFCPGSELTKLELTILLHHFILNYKMERVNPKCC 475

[Close]

BLAST of CaUC09G169850 vs. ExPASy TrEMBL
Match: A0A1S4E5L3 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503515 PE=3 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 8.4e-88
Identity = 168/227 (74.01%), Postives = 182/227 (80.18%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTE---VKCQMEKLIVATDENGTKLYDNNAIIDLLL 60
           ARKEIGNILE VIEEKR+ LE+ + T+   V+CQM+KLIVA DENG +LYDNNAIIDLLL
Sbjct: 214 ARKEIGNILECVIEEKRRVLERNKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLLL 273

Query: 61  GLLHAGHHTPAYAAMWALVHISENSHVFQKA----------------------------- 120
           GLLHAGHHTPAYAA WA+V IS+NSHVFQKA                             
Sbjct: 274 GLLHAGHHTPAYAATWAVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 333

Query: 121 -KFINELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDP 180
            KF+NELLRR+ IAVASFREA T+VYINDYTIPKGW V IWTRAIHMDPQ+YSNPQEFDP
Sbjct: 334 MKFVNELLRRYAIAVASFREATTNVYINDYTIPKGWIVTIWTRAIHMDPQMYSNPQEFDP 393

Query: 181 SRWDNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKME 195
           SRWDNYTPKPGEFIPFG+GSRFCPGSELTKLEITILLHHFILNYKME
Sbjct: 394 SRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFILNYKME 440

[Close]

BLAST of CaUC09G169850 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 205.3 bits (521), Expect = 5.1e-53
Identity = 103/249 (41.37%), Postives = 150/249 (60.24%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK++    +S++  +R   ++  ++  K  ++ LI   DENG ++ D+  IIDLLL  L
Sbjct: 239 ARKKLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYL 298

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKAK-----------------------------FI 120
           +AGH +  +  MWA + + E+  + QKAK                              I
Sbjct: 299 NAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQKLTLKETREMVYLSQVI 358

Query: 121 NELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWD 180
           +E LR  T ++ +FREAK+DV ++ Y IPKGW V  W R +H+DP+IY +P++FDPSRW+
Sbjct: 359 DETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 418

Query: 181 NYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKLV 221
            YTPK G F+PFG+GS  CPG++L KLEI+I LHHF+L Y++ER NP C +  LP  +  
Sbjct: 419 GYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPK 478

[Close]

BLAST of CaUC09G169850 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 205.3 bits (521), Expect = 5.1e-53
Identity = 103/249 (41.37%), Postives = 150/249 (60.24%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK++    +S++  +R   ++  ++  K  ++ LI   DENG ++ D+  IIDLLL  L
Sbjct: 239 ARKKLVAAFQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYL 298

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKAK-----------------------------FI 120
           +AGH +  +  MWA + + E+  + QKAK                              I
Sbjct: 299 NAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQKLTLKETREMVYLSQVI 358

Query: 121 NELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWD 180
           +E LR  T ++ +FREAK+DV ++ Y IPKGW V  W R +H+DP+IY +P++FDPSRW+
Sbjct: 359 DETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 418

Query: 181 NYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPKLV 221
            YTPK G F+PFG+GS  CPG++L KLEI+I LHHF+L Y++ER NP C +  LP  +  
Sbjct: 419 GYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPK 478

[Close]

BLAST of CaUC09G169850 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 198.4 bits (503), Expect = 6.2e-51
Identity = 100/249 (40.16%), Postives = 147/249 (59.04%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK +    +S++ E+R   ++   +  K  ++ L+   DE+G K  D+  IID+LL  L
Sbjct: 240 ARKTLVAAFQSIVTERRNQRKQNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYL 299

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKAK------------------------------F 120
           +AGH +  +  MWA V + E+  V Q+AK                               
Sbjct: 300 NAGHESSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQV 359

Query: 121 INELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRW 180
           ++E LR  T ++ +FREAKTDV +N Y IPKGW V  W R +H+DP+++ +P++FDP+RW
Sbjct: 360 VDETLRVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARW 419

Query: 181 DN-YTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAPK 219
           DN + PK G F+PFG GS  CPG++L KLEI+I LHHF+L Y+++R NP C + +LP  +
Sbjct: 420 DNGFVPKAGAFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTR 479

[Close]

BLAST of CaUC09G169850 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 116.7 bits (291), Expect = 2.4e-26
Identity = 79/235 (33.62%), Postives = 109/235 (46.38%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARKE+  IL +++  KR+      T  +   ME     TDE          I D ++G++
Sbjct: 224 ARKELAQILANIL-SKRRQNPSSHTDLLGSFMEDKAGLTDEQ---------IADNIIGVI 283

Query: 61  HAGHHTPAYAAMWALVHISENSHVFQKA-----------------------------KFI 120
            A   T A    W L ++++N  V +                               + I
Sbjct: 284 FAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVI 343

Query: 121 NELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWD 180
            E LR  TI   +FREA  DV    Y IPKGW V    R IH +  I+S+P +FDPSR++
Sbjct: 344 QETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE 403

Query: 181 NYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLP 207
              PKP  F+PFG G   CPG+EL KLEI++L+HH    Y+   V P   I + P
Sbjct: 404 -VAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGP 447

[Close]

BLAST of CaUC09G169850 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 112.8 bits (281), Expect = 3.4e-25
Identity = 79/238 (33.19%), Postives = 116/238 (48.74%), Query Frame = 0


Query: 1   ARKEIGNILESVIEEKRKALEKKETTEVKCQMEKLIVATDENGTKLYDNNAIIDLLLGLL 60
           ARK++  I+  +I E+R+  ++   T+    +  L+   +E G ++     I D ++G+L
Sbjct: 223 ARKQLKTIVSEIICERRE--KRALQTDF---LGHLLNFKNEKG-RVLTQEQIADNIIGVL 282

Query: 61  HAGHHTPAYAAMWALVHISENSHVF------QKA------------------------KF 120
            A   T A    W L ++ ++  +       QKA                        K 
Sbjct: 283 FAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKV 342

Query: 121 INELLRRHTIAVASFREAKTDVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRW 180
           I E LR  +I   +FREA  DV    Y IPKGW V    R IH +P+ +SNP+ FDPSR+
Sbjct: 343 IVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRF 402

Query: 181 DNYTPKPGEFIPFGIGSRFCPGSELTKLEITILLHHFILNYKMERVNPRCDITHLPAP 209
           +   PKP  F+PFG G   CPG+EL KL+I I LHH + N++ E       I + P P
Sbjct: 403 E-VNPKPNTFMPFGSGVHACPGNELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFP 453

The following BLAST results are available for this feature:

BLAST of CaUC09G169850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877829.1	2.3e-111	82.40	beta-amyrin 11-oxidase-like [Benincasa hispida]	[more]
XP_011652661.1	3.9e-103	76.28	beta-amyrin 11-oxidase [Cucumis sativus]	[more]
XP_023511598.1	4.7e-101	74.21	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023511599.1	4.0e-100	74.21	beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022952148.1	1.2e-99	74.21	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]

BLAST of CaUC09G169850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	5.1e-58	45.06	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q43246	4.5e-54	41.43	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]
Q5VRM7	4.2e-52	39.68	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q9C5Y2	7.2e-52	41.37	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q9AXH9	2.1e-51	40.32	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]

BLAST of CaUC09G169850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GJP8	5.6e-100	74.21	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 S...	[more]
A0A6J1GLR1	1.6e-99	74.60	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S...	[more]
A0A6J1GJM1	2.1e-99	72.22	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S...	[more]
A0A6J1I8I5	4.9e-96	70.88	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=...	[more]
A0A1S4E5L3	8.4e-88	74.01	beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503515 PE=3 SV=1	[more]

BLAST of CaUC09G169850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	5.1e-53	41.37	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	5.1e-53	41.37	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	6.2e-51	40.16	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT5G45340.1	2.4e-26	33.62	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT3G19270.1	3.4e-25	33.19	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 9..29
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 90..209 coord: 2..89
None	No IPR available	PANTHER	PTHR24286:SF289	ENT-KAURENOIC ACID OXIDASE 2	coord: 90..209 coord: 2..89
IPR002397	Cytochrome P450, B-class	PRINTS	PR00359	BP450	coord: 134..149 score: 33.61 coord: 161..170 score: 43.56 coord: 89..100 score: 33.89 coord: 106..133 score: 26.67 coord: 170..181 score: 38.33
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 62..79 score: 25.98 coord: 89..100 score: 31.43 coord: 170..181 score: 31.64 coord: 161..170 score: 56.32
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 89..202 e-value: 3.1E-32 score: 111.9 coord: 1..88 e-value: 3.0E-6 score: 26.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..215 e-value: 7.9E-51 score: 175.0
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..202
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 163..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC09G169850.1	CaUC09G169850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0005783	endoplasmic reticulum
molecular_function	GO:0051777	ent-kaurenoate oxidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen